LOCUS       BGC0001788             11509 bp    DNA     linear   BCT 10-OCT-2016
DEFINITION  Streptococcus suis strain 65 lantibiotic gene cluster, complete
            sequence.
ACCESSION   BGC0001788
VERSION     BGC0001788
KEYWORDS    .
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 16:17:55
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 11509
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..11509
                     /db_xref="taxon:1307"
                     /mol_type="genomic DNA"
                     /organism="Streptococcus suis"
                     /strain="65"
     misc_feature    1..11509
                     /note="lantibiotic gene cluster"
     protocluster    1..11509
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[3948:6729]"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3949..6729
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     cand_cluster    1..11509
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..11509
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001788"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11509
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(461..1816)
                     /gene="sssK"
     CDS             complement(461..1816)
                     /codon_start=1
                     /gene="sssK"
                     /gene_functions="regulatory (smcogs) SMCOG1003:sensor
                     histidine kinase (Score: 94.4; E-value: 1.4e-28)"
                     /gene_kind="regulatory"
                     /product="sensor histidine kinase"
                     /protein_id="AOT85896.1"
                     /transl_table=11
                     /translation="MNIFKKNILKFILSFIFIIMVDIVLLTVAANYIRSQQSASNIIET
                     VSSEIVLSDGNYTVSQKAKELIDNNNLWIMIIDKNSGKEKFNIDKPKDIANQFDFADVI
                     RFSRFYLKDYPVFTQIKENDQDIYIIAFPKDSIIRYSNNYFDLSRIQIFPIIILSIIFA
                     NILFCLILYIYSITFLDRNIKPIISAIVKLPNGVNTQVKSVKELDKLTFAINSANQKLR
                     ENEEFKEDWISGIAHDIKTPLSVIVSNISLAIEKTDNDTLLKYLTPTLVESHYIQNLLN
                     DLNIFARLTNGNLILKQEIIYIIPFFKDIIIQIINQEIWHDFNFEFTADENLYNKKMYV
                     EKSLVSRIIHNLIYNSVLHNPSGCDIQIILKSLTNDRFSITILDNGVGTSVDRLNNINK
                     IEEFNFDISGVRRSGIGLKITKQIVDLHGGDMFISSQQGEYFQTTITLDSIG"
     gene            complement(1832..2530)
                     /gene="sssR"
     CDS             complement(1832..2530)
                     /codon_start=1
                     /gene="sssR"
                     /gene_functions="regulatory (smcogs) SMCOG1008:response
                     regulator (Score: 180.9; E-value: 4.3e-55)"
                     /gene_kind="regulatory"
                     /product="response regulator"
                     /protein_id="AOT85897.1"
                     /transl_table=11
                     /translation="MGFEIQLLNKHILIVDDDIALSESIKEVLMSRGFKNISFAYSISE
                     GIDIFSVSQIDLIILDVMLPDGEGYLLSKYVRKTSDVPILFLTAKNNPDDEINGLDSGG
                     DDYVTKPFLPKSLTYRIMALLRRAYRDESELIELTDCTIDLNNASVKKSNQNFSLTPTE
                     IQILRKLFTNKNYIVSTETICDTIWGLDSFGYEKSLMVHIRNIREKIEIIPSKPKHLIT
                     VKGLGYKLAI"
     gene            2885..3040
                     /gene="sssA"
     CDS             2885..3040
                     /codon_start=1
                     /gene="sssA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) L_biotic_A"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) leader_d"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /product="suicin 65 precursor"
                     /protein_id="AOT85888.1"
                     /sec_met_domain="L_biotic_A (E-value: 1.9e-31, bitscore:
                     97.3, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="leader_d (E-value: 6.2e-08, bitscore:
                     22.1, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKDNYELMNTIQEVSLEELDQIIGAGKNGVFKTISHECHMNSWQF
                     LFTCCS"
     CDS_motif       2885..2959
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MKDNYELMNTIQEVSLEELDQIIGA"
                     /locus_tag="sssA_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       2960..3040
                     /prepeptide="core"
                     /locus_tag="sssA_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="5.20"
                     /molecular_weight="2915.3"
                     /monoisotopic_mass="2913.3"
                     /core_sequence="GKNGVFKTISHECHMNSWQFLFTCCS"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MKDNYELMNTIQEVSLEELDQIIGA"
                     /leader_location="[2884:2959](+)"
                     /alternative_weights="2933.4"
                     /alternative_weights="2951.4"
     gene            3069..3224
                     /gene="sssA'"
     CDS             3069..3224
                     /codon_start=1
                     /gene="sssA_"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) L_biotic_A"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) leader_d"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /product="suicin 65 precursor"
                     /protein_id="AOT85889.1"
                     /sec_met_domain="L_biotic_A (E-value: 1.6e-30, bitscore:
                     94.3, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="leader_d (E-value: 6.2e-08, bitscore:
                     22.1, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKDIELMSTIQEVSLEELDQIIGAGKNGVFKTISHECHMNSWQF
                     LFTCCS"
     CDS_motif       3069..3143
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MKKDIELMSTIQEVSLEELDQIIGA"
                     /locus_tag="sssA__lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       3144..3224
                     /prepeptide="core"
                     /locus_tag="sssA__lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="5.20"
                     /molecular_weight="2915.3"
                     /monoisotopic_mass="2913.3"
                     /core_sequence="GKNGVFKTISHECHMNSWQFLFTCCS"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MKKDIELMSTIQEVSLEELDQIIGA"
                     /leader_location="[3068:3143](+)"
                     /alternative_weights="2933.4"
                     /alternative_weights="2951.4"
     gene            3763..3930
                     /gene="sssA1"
     CDS             3763..3930
                     /codon_start=1
                     /gene="sssA1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) L_biotic_A"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /product="hypothetical protein"
                     /protein_id="AOT85890.1"
                     /sec_met_domain="L_biotic_A (E-value: 2.6e-24, bitscore:
                     74.4, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNAKTNKEINYEAVATLQEVKIDDLDILLGGAGHGVNTISAECRW
                     NSLQAIFTCC"
     CDS_motif       3763..3855
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MNAKTNKEINYEAVATLQEVKIDDLDILLGG"
                     /locus_tag="sssA1_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       3856..3930
                     /prepeptide="core"
                     /locus_tag="sssA1_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-3.80"
                     /molecular_weight="2509.8"
                     /monoisotopic_mass="2508.2"
                     /core_sequence="AGHGVNTISAECRWNSLQAIFTCC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MNAKTNKEINYEAVATLQEVKIDDLDILLGG"
                     /leader_location="[3762:3855](+)"
                     /alternative_weights="2527.8"
     gene            3949..6729
                     /gene="sssM"
     CDS             3949..6729
                     /codon_start=1
                     /gene="sssM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     641.7; E-value: 3.5e-194)"
                     /gene_kind="biosynthetic"
                     /note="involved in lantibiotic modification"
                     /product="synthetase"
                     /protein_id="AOT85891.1"
                     /sec_met_domain="DUF4135 (E-value: 5.3e-77, bitscore:
                     248.8, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 9.7e-56, bitscore:
                     178.3, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVGSEILYNQFNTFQKVVLERYFPELLLEDGDIIDEIGKKILDYY
                     RPTLIYLINEKRIEGSLVGSTPEIRYDFFNNVLCRKGIILDEIEQRFPEINHRVVLSIQ
                     KYLSLVEFVKNTFISDFSELVAKKYINSTCVTPNISDIKLNVTGDIHNGDGVCIVSYRG
                     QKVVLKKKSAKPNILLARLDSRVSAYLDKEIHFIPSFLNKGNYFWEKFVISKPINSKNE
                     AEEFYRRIGYLLGYSYILNISDLHFENLISSNLTPILVDVETIFSISPFDTLVGNKATF
                     HIIEKSRDSVLSTGLLPIADSDDVFGGDTSGVLGGTFFGEVKVVVNHNRDDIRIEKKKY
                     QIKNQEHLPYFLNSGGEKHYLNAVEYIEFIKEGFRELGNFFVKEKQYLKNLYLNHSDIQ
                     TRILFRNTKDYSLIRQLLISPVYCDKSKVLFEKMSNKLNEYDCDMLIQSEKNQLLNMDI
                     PYFSTSINSCDITDGERKIWKLKISALNTALKKLERLSDELIEEQIDLIEFSLKTTQAL
                     YSTELQEEYRKYDCTSVDDGILNEGINALVDIILDDEKYSLEDDSTNWLTLKVNDHDAF
                     ELVPMDNSVYEGIAGMAIALSEAYDLVDPSRQERIMDCLKRILSTLLNLYTKIENPTYF
                     VGKLGVFSAISRISLITGQRLPDDIFKIRDQYCLNLNTSQADFLSSFPNEIVAFRNTDT
                     NIENLAQSLVKLKELGVNNNDYICWNRLEANNVSLAHGNLGVEVALLFLAYKSNRSEAL
                     DLFNKARTFENQQKLGEGWIDKRNGETSANWCHGSTGVLVARLAQLQLNDKYNILSDSE
                     VEELRRDVEHAVKQIIEIGFDMTNFSLCHGTSGNLLALSYYLNYMENCQSKKLKGIVDR
                     EYKKLHSFGLEKGWMCSFNTKYNVYGLMTGIPGILFSTAKYLKEDKSLDILIPSV"
     gene            6752..8824
                     /gene="sssT"
     CDS             6752..8824
                     /codon_start=1
                     /gene="sssT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 131.7; E-value:
                     5.7e-40)"
                     /gene_kind="transport"
                     /product="ABC transporter"
                     /protein_id="AOT85892.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 5.5e-40, bitscore:
                     125.7, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIILQNNEEDCLLACYTMMLNDLGHRIPLHEVCNKDSLPADGLS
                     LSYLLTLNDRFGVTINAYRTSFDDLLKIYEETKRRMIIHWNNDHFVVLEKIKTDRVLIV
                     DPAIGRIRYHKSEFMEHYSGTMIMVNKGNEFKPERYNPIFWSYFKKTLQVKPISLFILS
                     LLFIQASVLLFSINLRQMLTDEFKFSANLFLLSFVLVFQLLGYFVKNKALNKYNRDFDK
                     YYSKELFIRLLKKPLLYFRNHLSGGVSEKINLKSILRDNVTLKIIPSFISLISALVIFI
                     YLMTISVKLTVILVVMISVYSIFSILLYYKQSEYNQTYLQYLIDFNSELQTDLDDIDYI
                     KIMRKEDKIFNSWVTVNNKVTTKYSQILRIENLSQLIGTIFNYISLSSIIVISVYYNNY
                     IEVSLSDLLVYQTSISLLISAIEQVKGAIFEILRLEIYAEKQSDLLKDSAPITVPSSES
                     NDYLIQTDNLNFSYGNKPIYKNINLTVKKGEKIAIVGKSGSGKSTLLLLLAGMLRYNGS
                     IRYGIVGFEDYISVVLQNMTLRKGSILENLEWTSNDLSPLYQILKDTAADEVISQLPNK
                     IYSKLLKQGKNLSGGQIQKLLIAKSLIKDNGIVFWDEAFSNLDEHSKNRIYENVLQSSR
                     YSDKTMLIVSHHLDIVNFVDNVIFIDEESGEVIKDSHKNLMETNVNYGKFICSSF"
     gene            8934..9842
                     /gene="sssF"
     CDS             8934..9842
                     /codon_start=1
                     /gene="sssF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 172.4; E-value:
                     2.1e-52)"
                     /gene_kind="transport"
                     /product="immunity protein"
                     /protein_id="AOT85893.1"
                     /transl_table=11
                     /translation="MKNVIELRRLSKVYKDITTVDLEKITVREGEIYGFLGPNGAGKTT
                     TMKMILSLISPTSGEIIVTGSNIQNGKGYLKQIGSMIEEPSYYPNLTGYENLMVFQKMV
                     GFAKENIWPTLELVGLSEEKNRKKLVRDYSLGMKQRLALAFALVKKPKILLLDEPTNGL
                     DPAGIHEIRELIVKLAKEEGLTVFISSHILTEIEYIADRVGIINHGRLVYEGEIEAIKA
                     NRWVEIGGDFSQNNIVQSLVEFGSVEVLGATASYVKISDIDNDKLADVVSYLTKSGYRI
                     FRVARESETLEDIFLELTKEV"
     gene            9844..10608
                     /gene="sssE"
     CDS             9844..10608
                     /codon_start=1
                     /gene="sssE"
                     /product="immunity protein"
                     /protein_id="AOT85894.1"
                     /transl_table=11
                     /translation="MRGIYIEWLKSKRTKSFSIVTILIIVATLWNIVTFNSAFSSHPEL
                     KGVEILFSNQNVNLLMLPIAVCVFAARIVWNEREGQTFKLQFSNGHNLLTIFANKFLFM
                     VIIFSFMSILEVIAICIFGQQVGIRIPFSVILFQLFAQFLSVYSLICIYLFLAIVVEKQ
                     GLLLALGLLGGFLGIVLTPRSYGFSSLINPITGFGSLAPYKYQSLGGGEFAYIFDSQLF
                     WKLIIYSVYCLLLYAIANLVLRKRGNKDGKLF"
     gene            10592..11326
                     /gene="sssG"
     CDS             10592..11326
                     /codon_start=1
                     /gene="sssG"
                     /product="immunity protein"
                     /protein_id="AOT85895.1"
                     /transl_table=11
                     /translation="MGSYFKAEWKKARKIQLLLIGMAFLTFSSFIGLGIYFSNRDVLVE
                     GTQSLVLWGQLTFYNSTLLYPPMLAIIAGLLIIPEFERKNLEMLKANQVSMWKLYFGKL
                     LCSFFIILPVQLVLLIIFIIAIHADGIILDLSLQTYIKWLLLSLLASVPIIILQSYITV
                     KTRNFSKSIGIATVGSVLNFVLIFINEDLTKFFPYSQPMVALRSRALTDMSFGNLSIFL
                     VVNSIFSILFYKLTVDVLEKNN"
ORIGIN
        1 ggngttagac gactggtcgc agaatagcag agctagatgt caaactaaat caaaccaatg
       61 aaaaaatcgc caccttgaat aagatggcgg aagtgcttat caacctaaag agtgatgatc
      121 cgaatagtcg aaaactagca aggtatgact tttcaaaact aaatttgaca gaatcaatta
      181 cactagaaca agtgactgaa gaaattaagc tattacaaga aaaatttagc ttatatctgg
      241 atgagtatga agggctggta agtagaatag aaaggtttgt aaaaattctt aatacagata
      301 tagacttgaa attccaagag aatgtctcac tagaataaac tattacctat aggtttaatt
      361 aataaaaatc aaggagagtt tttgctataa aaaataagga agaaatctat cgaaagaatt
      421 gataattggg cgattgataa aggtatcctc aaaaatatct tcaaccaata ctatctaaag
      481 taatagtagt ttggaagtat tctccctgtt gactagatat aaacatatca ccaccatgca
      541 aatcaacaat ttgcttagta atttttaagc ctataccact cctacgaact ccagaaatat
      601 caaaattaaa ttcttcaatt ttattgatat tgttaagtct atccacagat gttcccactc
      661 cattatctag tatagtaatt gaaaatctgt cattggtcag agatttaaga ataatctgaa
      721 tatcacatcc agaggggtta tgtaaaacag aattgtaaat caaattatgg attatcctag
      781 aaacaagaga tttttcgaca tacatttttt tgttatataa attttcatcg gcagtaaatt
      841 caaaattgaa atcgtgccaa atttcttgat taataatttg aatgattata tctttaaaaa
      901 atggaataat ataaataatt tcttgtttca atatcagatt tccgtttgtt aatctagcaa
      961 atatatttag gtcattgaga aggttctgga tataatgact ttcaactaaa gtaggagtta
     1021 aatatttgag tagagtgtcg ttatcagttt tctcaatggc taaagatatg ttggatacaa
     1081 taactgaaag aggagttttt atgtcgtgtg caattccaga aatccaatct tctttaaact
     1141 cttcattttc acgaagtttc tgattggcag aattaatggc gaatgttaat ttatctagtt
     1201 cttttactga tttaacttgt gtatttacac cgttcggtaa ttttacaatt gcagaaataa
     1261 ttggcttgat atttctatct agaaaagtaa tactataaat gtataaaatt aagcaaaata
     1321 atatgttagc aaaaataatt gataggataa tgatgggaaa aatttgaatc ctgctcaaat
     1381 caaaataatt attactatag cgaataatac tatctttcgg gaatgcaata atatatatat
     1441 cctgatcatt ttcttttatt tgagtaaata caggatagtc ttttaaataa aatctggaga
     1501 aacgaataac atctgcaaaa tcaaattgat tagctatatc tttaggttta tcaatattaa
     1561 atttttcttt tccactattt ttgtcaataa tcataatcca taagttatta ttatcgatta
     1621 attctttggc tttttgactg acagtataat taccatcaga taaaacaatt tctgatgata
     1681 ctgtttctat aatgttggag gcagactgtt ggctacgtat atagtttgct gctacagtta
     1741 gaagtactat atcaaccatt atgatgaaaa taaacgataa aataaatttt aatatattct
     1801 ttttgaaaat attcataaga tctcttattg tttaaatagc aagtttatat ccaagccctt
     1861 taacggtaat tagatgcttt ggcttggaag gtataatttc gattttttct cgaatattgc
     1921 gtatatggac cattaaggat ttttcatatc caaagctatc taatccccaa atagtatcac
     1981 atattgtctc tgtggaaacg atgtaatttt tgttggtaaa tagctttcta agaatttgaa
     2041 tttcagtagg ggtcagtgaa aaattttgat tacttttttt tacgcttgca ttattcaagt
     2101 caattgtgca atctgttaat tcaatgagtt cagattcatc tcggtaagct cttctcaaca
     2161 atgccattat acggtaagtt aaagatttag gtaaaaatgg ctttgtaaca tagtcatctc
     2221 ctcctgagtc aagcccattt atttcatcat caggattatt tttagcagtt aagaatagaa
     2281 ttgggacatc agatgtcttt cttacatatt tggaaagtaa atagccttca ccatctggta
     2341 acatgacatc tagaataatt aaatcaattt gagatactga aaaaatatca ataccttcgc
     2401 taatactata agcaaaacta atatttttga agccacgact catgagcact tctttgattg
     2461 attcgcttaa tgcgatgtca tcatccacaa ttagtatatg tttattcaac aattgtattt
     2521 caaaacccat acttttacct ccaatgttta tataaattat atcatacaat aaagttatcg
     2581 ggtttattta gccttactca gaatgaaaat ataataagca tacaaatatt ttatgacttg
     2641 acaatcatat aataaagatt gaaataatat ttaatgttgt aattcgaaaa ataaaacaag
     2701 ttatagataa aaatatatta tttcaagggc ctcactaact aaattaaaat atttggagat
     2761 taaaaatctt aaagttaaat ttatgttttc tttatacaga ctttaagata ctcctgtaag
     2821 atgaacttgt aacaaagaag atatcgatat tgtcggtaat aaaaaataga aagaggaaaa
     2881 tgttatgaaa gataactatg aattaatgaa tacgattcaa gaagtaagtc ttgaagagtt
     2941 ggatcagatt attggtgctg gaaaaaatgg agtttttaaa actatctcac atgaatgtca
     3001 catgaattca tggcagtttt tattcacatg ttgctcgtaa aaaaattttt caaagagagg
     3061 cgactaaaat gaaaaaagat attgaattaa tgagtacgat tcaagaagta agtcttgaag
     3121 agttggatca aattattggt gctggaaaaa atggggtttt taaaactatt tcgcatgaat
     3181 gtcatatgaa ttcatggcaa ttcctattta cgtgttgttc ataaatatat acatttagat
     3241 atctttgaag ttacgtaatg gaggatttcc tctgacaatt tacagacaga ctcaaaatta
     3301 ttttggtctg tctttttttg ataacgctgg gcatggctac atttaagatg aaagtactct
     3361 ggggacacct gctagcgagt aacccaatag gactcaaaat gctgactatt atgaggtggc
     3421 aaggataggg aggaatagtg aaatggtggt tctctgaaca gaaatgttat gtaaggtata
     3481 tatggaggat gagaagacca aaaattctaa atacctagaa acctacctag caatgtgaga
     3541 gttagggagc tcatatcttg tgtaaacgag gaaagatgca ggacttatcc ctagaggtct
     3601 catgtgaagt tgatcgttgc catagaagtg acgaatattt tgtaatgtat aaatttattt
     3661 aaaatttatt atttcttaaa cttatcttta agtaagagta ctaaaattat catatagaga
     3721 agtgcactga ctaaactaat attaaaaaga aggtaaaaaa ttatgaatgc gaaaactaat
     3781 aaagaaataa attatgaagc agttgctaca ctacaagaag taaaaatcga tgatctggat
     3841 attcttttag gaggagcagg tcatggtgtt aatactattt ctgcggaatg tcgttggaat
     3901 agtttacagg caatcttcac atgttgttag tttttggagg ggaacagtat ggtgggtagc
     3961 gaaattttgt acaaccaatt taatactttt caaaaagtag ttcttgaaag atactttcca
     4021 gaactattac tggaggatgg tgatatcatt gatgagattg ggaaaaagat tttagattat
     4081 tatcgtccga cgctaattta tttaataaat gagaagcgga ttgaaggaag tctagtaggg
     4141 tctacaccag agattcgtta tgactttttt aataatgtct tatgtaggaa aggtataata
     4201 ttggatgaga ttgagcaacg tttcccagaa ataaatcatc gcgttgtact cagtattcaa
     4261 aaatatttaa gtttggttga atttgtaaaa aatacattta tttctgactt ttcagaatta
     4321 gtagcaaaaa agtacattaa tagtacgtgc gtgactccta acataagtga tatcaaactt
     4381 aatgttactg gagatattca caatggtgac ggagtatgta ttgtatctta ccgaggacaa
     4441 aaggttgtat taaaaaagaa atcagctaaa ccaaacatac ttttggctcg attagattct
     4501 agagtcagtg cttatctaga taaagaaatt cattttattc ctagtttttt gaataaagga
     4561 aattattttt gggaaaaatt cgtaattagt aagcctatta attcaaaaaa tgaagctgag
     4621 gaattttatc gtagaatcgg ttatttatta ggatattcct acattttaaa tatttcagac
     4681 ttacattttg aaaatttaat ctcttcaaat cttacaccta ttttggttga tgtcgaaaca
     4741 attttttcta tctcaccatt tgatacgttg gttggtaata aagcaacatt tcatattata
     4801 gaaaaaagta gagactccgt actttccact ggtcttcttc caattgctga ctcagatgat
     4861 gtttttggcg gggatactag tggagttctt gggggaacat tctttgggga agtaaaagtt
     4921 gttgttaatc ataatcggga tgacatccgt atcgaaaaga aaaaatatca gataaaaaat
     4981 caagaacatt taccctattt tcttaattca ggaggagaga agcattatct taatgcggta
     5041 gaatatattg aattcattaa agagggattc cgagaattgg ggaatttttt tgtcaaggaa
     5101 aaacaatacc ttaaaaatct ttatttaaat catagcgata ttcagactag aattttattt
     5161 cgaaacacta aggattatag tttaatacga caattactaa tatctcccgt atactgcgat
     5221 aagtcgaaag tgctatttga aaaaatgtca aataaactaa atgaatatga ctgtgatatg
     5281 ttaatccagt cagaaaaaaa tcagctatta aacatggata ttccatattt tagcacaagc
     5341 attaatagct gtgatattac agatggagag agaaaaattt ggaaattaaa aatctcagcg
     5401 ttaaatacgg ctcttaaaaa gttagagagg cttagtgatg aattaattga ggaacagatt
     5461 gatttgatag aattttcact aaaaactaca caagctttgt atagtacaga gcttcaagaa
     5521 gaatatagaa aatatgactg tactagtgtt gatgacggta tattaaatga aggtattaat
     5581 gcgttagtag atatcatctt ggatgatgag aaatattcat tggaagatga tagtacaaat
     5641 tggttaactt taaaagttaa tgatcacgac gcttttgaat tagttccaat ggataattca
     5701 gtatacgaag gaatcgctgg catggctatt gctttatctg aagcttatga tttagttgat
     5761 ccttctagac aagagagaat tatggattgt ttgaagcgaa tactatcaac tttattaaat
     5821 ttatatacta aaatagaaaa tccaacatac ttcgttggaa aattaggagt tttttctgct
     5881 atttctagaa ttagcttgat tacaggtcag agacttccag atgatatatt taaaattaga
     5941 gatcaatatt gtttaaattt aaatacatcg caagctgatt ttttatcaag ttttccgaat
     6001 gaaattgttg cattcaggaa tacagacaca aatatagaga atctagctca atcactagtt
     6061 aaacttaaag aattaggtgt caataacaat gattatatat gttggaatcg attagaagca
     6121 aataatgtca gccttgccca tgggaattta ggagtagaag tagccttgtt gtttttagca
     6181 tataaatcta atcgttcaga ggccttagat ctatttaata aggctagaac ttttgaaaat
     6241 caacaaaaac taggtgaagg ctggattgat aaaaggaatg gtgaaacaag tgcgaattgg
     6301 tgccacggtt ctacaggggt attagttgca aggttggcac agctgcaatt aaatgataaa
     6361 tataatattt tatctgattc tgaggtagaa gaattgagaa gagatgtaga gcatgctgta
     6421 aaacaaatta ttgaaattgg ttttgatatg actaattttt cgctgtgcca tggtaccagt
     6481 ggaaatttat tagctctcag ttattattta aattatatgg agaattgcca gtccaaaaaa
     6541 ctcaaaggta ttgtagatcg agaatacaaa aagttacatt catttggttt agaaaaaggc
     6601 tggatgtgca gctttaatac aaaatataat gtttatggct taatgactgg gattccagga
     6661 attttgtttt ctacagctaa atatctaaaa gaagataaat cgcttgatat tcttattcct
     6721 agcgtatgag ttacagaaag gaagaaagac gatgaagatt attttgcaaa acaatgaaga
     6781 agattgtctt ctagcttgtt atactatgat gttgaatgac cttggacacc gaatcccttt
     6841 acatgaagtc tgtaataaag attcattacc agcagatggt ctaagtttat cttatctact
     6901 aactttaaat gatagatttg gagtgactat aaatgcttat cgaacttcat ttgatgattt
     6961 attgaaaatt tacgaagaaa ccaaaagaag aatgattatt cactggaata acgatcattt
     7021 tgtagttcta gaaaaaatta aaactgaccg agtcctaatt gtagatcctg caattggccg
     7081 aattagatac cataaaagtg agtttatgga acattattct ggaacgatga ttatggtaaa
     7141 taaaggtaac gagtttaagc cagaaagata taaccccatt ttttggagtt attttaagaa
     7201 gacacttcag gtcaaaccca tttccttatt tattttatct ttgttattta ttcaagcaag
     7261 tgtactattg ttttcaataa atttaagaca aatgctaaca gatgagttca aattcagtgc
     7321 aaatttattt ttactaagtt ttgttcttgt ttttcaactg cttggatatt ttgtaaaaaa
     7381 taaggcgctg aataagtata atagggattt tgacaaatat tatagtaaag agttatttat
     7441 aaggctttta aagaagcctt tgttatattt tcgcaatcat ttaagtggtg gggtttctga
     7501 aaagattaat ctaaaatcaa tattaagaga taatgttaca ttaaaaatta taccgtcgtt
     7561 tataagttta atttcagcct tggtaatttt tatctattta atgacaatct ctgtaaaatt
     7621 aacagtgatt ttagttgtga tgatctctgt ctatagtata ttttcgatac tgttatatta
     7681 taaacaaagc gaatacaatc aaacgtattt gcaatatctt atcgatttta attcggaatt
     7741 acaaaccgat ttggacgata ttgactatat aaagataatg agaaaagaag ataaaatttt
     7801 caattcttgg gtgacagtca ataataaagt cacaacaaaa tactctcaaa ttttgagaat
     7861 tgaaaatcta tctcaattga ttggaactat atttaactac atcagtttat cttctattat
     7921 agttatatca gtttattaca ataactatat agaagtatca ctttcggact tacttgtata
     7981 tcaaactagc atttcattat tgatttcggc aattgaacag gtgaaaggag caattttcga
     8041 aatacttcga ttggagattt acgctgaaaa gcaaagtgat ttgttgaaag actcagcacc
     8101 aattactgta ccgagttcag aatcaaacga ttatttaata cagactgata acttaaactt
     8161 ttcttatggt aataaaccaa tctataaaaa tattaatctt acagtcaaaa aaggagaaaa
     8221 aattgctatt gtaggaaaat cggggagtgg taaatctaca ttactgttac ttctagcagg
     8281 catgcttcgt tataatggct ctattagata tggaatcgta ggatttgagg attatataag
     8341 tgtggtttta cagaatatga cgcttagaaa aggaagtatt ttagaaaatc ttgaatggac
     8401 ttctaatgat ttatctcctc tttatcaaat attgaaagat acggcagcgg atgaagtaat
     8461 ctctcagcta cctaacaaaa tatattctaa attgttaaaa caggggaaaa atttatcagg
     8521 tggacaaatt cagaaattac ttatagcaaa atcattgata aaagataacg gcattgtttt
     8581 ttgggatgag gcgtttagta atttggatga acacagtaaa aatagaattt atgaaaatgt
     8641 attacagtct tctaggtatt cggataaaac aatgctgatt gtaagtcacc atttagatat
     8701 tgtgaatttt gttgacaatg ttatattcat tgatgaggag tctggtgaag tgattaaaga
     8761 tagtcataag aatctcatgg aaacaaatgt aaattatgga aaatttattt gttctagttt
     8821 ttaagtaaac cccagttctc ttaatgttat ctttaacttg tgacagtaca atagtagtga
     8881 aataaaaaag agaggaagag ccctaagagt aggcttatat taggtatgta gagatgaaaa
     8941 atgtaattga attgcgtaga ctatcaaaag tgtataaaga tattaccacg gtagacttag
     9001 aaaagattac cgtccgagag ggtgaaattt acggttttct tggaccaaat ggggcaggta
     9061 aaacaacaac tatgaaaatg atattgtctc tgattagtcc tacttcggga gaaatcatag
     9121 taactggctc taatatacaa aatggcaaag gctatctaaa acaaattggg tcaatgattg
     9181 aggaaccttc ttattatcct aatcttacag gttatgaaaa tttaatggtc tttcagaaga
     9241 tggtaggatt cgcaaaagaa aatatttggc caacattaga gttagtggga ttgtcagaag
     9301 aaaaaaatcg gaaaaaactt gtgagggatt attcacttgg tatgaaacaa agactagcac
     9361 ttgcttttgc gttggttaag aaacccaaaa ttcttttact tgatgagccg accaatggtc
     9421 ttgatccagc cggcattcat gaaattcgag aattaattgt taaattagct aaagaagagg
     9481 gattgacagt atttatttct agtcatattc ttacagagat tgagtatatt gcagacagag
     9541 ttggaattat caatcacgga cgtttggttt atgaaggaga aatcgaagct attaaagcta
     9601 ataggtgggt ggagattggt ggagattttt ctcagaataa tatagttcag agtttggttg
     9661 agttcggttc agtagaagta ttaggtgcaa ctgcaagtta tgtcaaaata agcgatattg
     9721 ataatgataa attagcagat gtagtaagtt atttaaccaa gagtggttat cgcattttta
     9781 gagtggccag agagagtgaa acactagaag atatttttct agagttaacc aaggaggtgt
     9841 agcatgagag gtatatacat tgaatggtta aaaagtaaac gaacaaaatc tttttcaata
     9901 gtaactattt tgattattgt tgcgacacta tggaatatcg ttacgtttaa ttctgcattc
     9961 tcttcccatc cagaattaaa aggggtagaa attttattta gcaatcaaaa tgtcaacctc
    10021 ctcatgcttc ctatagcagt ttgtgtattt gctgctcgca ttgtatggaa tgagcgtgag
    10081 ggtcagactt ttaagttgca attttcaaat ggtcataatc ttctaactat ttttgccaat
    10141 aaattcttat ttatggttat tatctttagc ttcatgtcaa ttctagaagt cattgctatt
    10201 tgtatttttg gtcaacaagt aggcatcaga atcccgttct cagttatttt atttcaactt
    10261 tttgcccaat ttctttcagt atattctttg atttgtatct atttattctt agctatagta
    10321 gtagaaaaac agggtctatt attggcatta ggtttgttag gtggtttttt aggcattgtt
    10381 ttgactccaa gaagttatgg cttcagtagt ttgattaatc ctataacagg atttggtagc
    10441 ttagcaccct ataaatatca gtctcttggt ggcggggagt ttgcatacat ttttgatagt
    10501 caattatttt ggaagttgat aatttattct gtttattgtt tattgttgta tgctatagcc
    10561 aatctcgttt tgagaaagag agggaataaa gatgggaagt tattttaagg cagaatggaa
    10621 aaaagctaga aaaatccaac ttttgttgat tggaatggct tttctgactt tttctagttt
    10681 tattgggtta ggaatatatt tttctaatag ggatgtttta gttgaaggaa cacaatcact
    10741 ggttttatgg ggacaattaa cattttataa tagcaccttg ctctatcctc cgatgcttgc
    10801 tattattgct ggtttactga ttatacctga atttgaacga aaaaaccttg aaatgttgaa
    10861 agcaaaccaa gtctcaatgt ggaaattgta ttttggtaaa ttattgtgta gttttttcat
    10921 tattcttcca gttcaactag tattattaat tatttttatt atagcaattc atgctgatgg
    10981 aattattctg gatttgagtt tacagacgta tattaaatgg ctattattgt cacttttagc
    11041 atctgttcca ataattattc ttcaatcgta tataacagtg aaaacaagaa atttcagtaa
    11101 aagtattgga attgcgacag ttggaagtgt attgaatttt gtacttatct ttatcaatga
    11161 agatttaaca aaatttttcc cttattctca gccaatggtt gcactccgaa gtagagcatt
    11221 aacagatatg tcttttggta atttatctat ttttctggta gtaaatagta tattttctat
    11281 ccttttttat aaattaacag tagatgtttt ggaaaaaaat aattagtttt aaataaatcg
    11341 cttctatata acatacaagt cactaggctt ttataggagt taaaaaagag gagaggtact
    11401 cctctttttt aataatctgt ttggtccaag ctggaccatt ttccgtcttt ttataatttg
    11461 tacagtatac tttttcttag gagaaagctt aatgtatcgg cgaataaga
//
