LOCUS       BGC0001789             13648 bp    DNA     linear   BCT 10-OCT-2016
DEFINITION  Streptococcus suis strain 90-1330 lantibiotic gene cluster, complete
            sequence.
ACCESSION   BGC0001789
VERSION     BGC0001789
KEYWORDS    .
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 16:18:00
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 13648
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..13648
                     /db_xref="taxon:1307"
                     /mol_type="genomic DNA"
                     /organism="Streptococcus suis"
                     /strain="90-1330"
     misc_feature    1..13648
                     /note="lantibiotic gene cluster"
     protocluster    1..13648
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[6814:12849]"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      6815..12849
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     cand_cluster    1..13648
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..13648
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001789"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..13648
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /region_number="1"
                     /rules="((LANC_like and (Lant_dehydr_N or Lant_dehydr_C))
                     and not (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            337..1710
                     /gene="sslP"
     CDS             337..1710
                     /codon_start=1
                     /gene="sslP"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 229.9; E-value: 1.2e-69)"
                     /gene_kind="biosynthetic-additional"
                     /note="involved in proteolytic cleavage of the leader
                     peptide"
                     /product="protease"
                     /protein_id="AOT85873.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 2.7e-39, bitscore:
                     124.7, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKIGLLSNVFISLFIVILASGSVSSGVSANENKEISYNVLVSEN
                     KSIEDVEDVLQSQGIKVTDKIPEINFLTISTEKSEDTIKKENSNYIDDIVIDNTMTVKP
                     NITYLYGDKVYTNTNLDFWGNQWDMQKSISTGSKFQHKSSGKATIGVIDSGITYDNPDI
                     YSHIISVQNFTSDLETGIVDNQNVVDKIGHGTSVVGQISSSGEYLGIAPDMKIRMYRVF
                     DEGNAQDQWILKALIQAAKDDVDVINLSLGEYLLRDSTDEDDDTALINIYQRAINYAHN
                     QGSVVVASVGDEGMDLQNQDELKNYLGSLRGKDYSQVDGTVQDIPAELDNVVTVGSVDD
                     NDLVSSFSNQGNGDIDIYATGGGSQALSKVGYDKWVADKLYEKEWILVPTLEGKYTYAY
                     GTSISTPKVSAALGLIIEKYNLKDQPDEAIRILYDNCFLSNNGNNGQIRLLNITNFVQ"
     gene            1751..2449
                     /gene="sslR"
     CDS             1751..2449
                     /codon_start=1
                     /gene="sslR"
                     /gene_functions="regulatory (smcogs) SMCOG1008:response
                     regulator (Score: 181.4; E-value: 3e-55)"
                     /gene_kind="regulatory"
                     /product="response regulator"
                     /protein_id="AOT85874.1"
                     /transl_table=11
                     /translation="MYKILVVDDDFEIVKLMRTILEMKNYNVTTYQEVRLPIKISDFDG
                     FDLILLDVMMPNVDGLQICKQIRKSVSSPIIFVSAKDSEDDIVSGLNLGGDDYITKPFS
                     VNQLVAKVAAHLKREERYRNAESNESVIRELLPLTFYLQEKLVRVNGEAISLTKREYDI
                     LELLSSKTNKVYTREEIYECVYDDEADALFRSISEYIYQIRVKFGSYGINPIKTIRGMG
                     YKWNDEKIFN"
     gene            2427..3791
                     /gene="sslK"
     CDS             2427..3791
                     /codon_start=1
                     /gene="sslK"
                     /gene_functions="regulatory (smcogs) SMCOG1003:sensor
                     histidine kinase (Score: 117.4; E-value: 1.6e-35)"
                     /gene_kind="regulatory"
                     /product="sensor histidine kinase"
                     /protein_id="AOT85875.1"
                     /transl_table=11
                     /translation="MMKRYSINKRILRTVLEIVIGTVANVIMLLVLSYVLIFTGQLNTY
                     GTDYHISISDSKSANGIKNQMDYSVFDYVLFDSDNGELVSGKYQKQDLQHYKEVFDTGS
                     SVSEGSILFTEYENSDFILVIRRPTVPEFVNQNFRHISYNLVSYLFFIVLELLLISISV
                     IRLLREFTKNFRLIEKISLNMGIPGMDIGRKETEILECNNILLTLYQKDEELTKLLEME
                     KKDKEDLSFQVAALAHDIKTPLTVLKGNIELLEMTNLDEKQLDFMSSINHSVGIFEKYF
                     NSMVNYSRLLIEDKNYQEDIELAKFLDELSLECRDIMSSKKINFQIVNESHTRVIKGNR
                     LNLDRALINILSNATRFCSEEGKVVLTISESSNFISFEIWNNGLPFTEESLKDGGKLFY
                     TENKGRNDKHYGIGLSFAQKIAMRHQGQLILSNPQNGGAQVTLLIKKILRAGQKA"
     gene            3948..4664
                     /gene="sslF"
     CDS             3948..4664
                     /codon_start=1
                     /gene="sslF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 181.6; E-value:
                     3.2e-55)"
                     /gene_kind="transport"
                     /product="immunity protein"
                     /protein_id="AOT85876.1"
                     /transl_table=11
                     /translation="MEAEKMEVIIQNLRKSYKDKEVLKDVSFKIEEGTICGLLGVNGAG
                     KSTIMKILFGLERADSGEVIFNGKHKDIVEENRFDIGALIESPAIYLNLSAFDNLKTRA
                     LLHDIPDERICEVLQLVGLSDTGKKKAGHFSLGMKQRLGLGMAIITNPDLLILDEPTNG
                     LDPDGIKELLNLIRDLKKSGMTVLLSSHQLSEVSKIADQIVILNQGKIFYDDINKNSND
                     LEKLFFRIVHGGDGND"
     gene            4642..5394
                     /gene="sslE"
     CDS             4642..5394
                     /codon_start=1
                     /gene="sslE"
                     /product="immunity protein"
                     /protein_id="AOT85877.1"
                     /transl_table=11
                     /translation="MEVMEMTNIFKSEWLKQKSSSDKKLLILIPLLAIFIAFILAGPLN
                     LESFSIYWWEGGFLFTLLGLLFLADYSAEENAGAFQNISLGRYTFTIYLAKILLKFKDV
                     LISTFIFTIIFYGISFLFSGVISVDIGKDLICLLLIGVSSAWVLPLLYFLAKWINPYLL
                     LATNSLICLLVAPLIAQTSVWFIFPYTYHYKIAYALLHLKPSGDLEAALSGVEISTILI
                     SVSLSILVFVSFLSLLKWRIINEQNSKK"
     gene            5372..6058
                     /gene="sslG"
     CDS             5372..6058
                     /codon_start=1
                     /gene="sslG"
                     /product="immunity protein"
                     /protein_id="AOT85878.1"
                     /transl_table=11
                     /translation="MNKILKSELLKLKGSLTLNLILILSIIQLFTIPLYLQFTNNSVVI
                     ENIIFLPMLGYCILASIFSIFLHEQEEKANFFQNIKSEKNSGIIWGIKLISTDLLMVLL
                     GVPVWIVVGVEFNRLSYFAYVGVITWLLLVLLNHFHMLLSLIMGKGGNLVISFIECLFI
                     IFATNKVFLNIFWLPIVLPVNMILEIGKNEIFMILVYLIGFIILSYFCNLAVMNNVEIQ
                     KICKKR"
     gene            6407..6574
                     /gene="sslA"
     CDS             6407..6574
                     /codon_start=1
                     /gene="sslA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_a"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /product="suicin 90-1330 precursor"
                     /protein_id="AOT85868.1"
                     /sec_met_domain="TIGR03731 (E-value: 3.1e-24, bitscore:
                     74.4, seeds: 23, tool: rule-based-clusters)"
                     /sec_met_domain="mature_a (E-value: 2.4e-08, bitscore:
                     22.8, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNEDFNLDLVTISKENNSGASPRVTSKSLCTPGCKTGILMTCAI
                     KTATCGCHFG"
     CDS_motif       6407..6478
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MNNEDFNLDLVTISKENNSGASPR"
                     /locus_tag="sslA_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       6479..6574
                     /prepeptide="core"
                     /locus_tag="sslA_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="27.40"
                     /molecular_weight="2989.6"
                     /monoisotopic_mass="2987.5"
                     /core_sequence="VTSKSLCTPGCKTGILMTCAIKTATCGCHFG"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MNNEDFNLDLVTISKENNSGASPR"
                     /leader_location="[6406:6478](+)"
                     /alternative_weights="3007.6"
                     /alternative_weights="3025.7"
                     /alternative_weights="3043.7"
     gene            6815..9790
                     /gene="sslB"
     CDS             6815..9790
                     /codon_start=1
                     /gene="sslB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155:Lantibiotic dehydratase domain protein (Score:
                     786.9; E-value: 4.1e-238)"
                     /gene_kind="biosynthetic"
                     /note="involved in lantibiotic synthesis"
                     /product="dehydratase"
                     /protein_id="AOT85869.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 2.1e-92, bitscore:
                     299.7, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 9e-09,
                     bitscore: 25.6, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 1.5e-22, bitscore:
                     70.0, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEHKYTTNNFLVRNAIIGIHELLECDYNSFLETIRENKIFQEQLF
                     VASRSLYESLQKYYSGDSMKRKKINQLSESVYKYYKRSKERSTPFGLFSETSIGSFSST
                     EKLNLNGKTLKKVLLDSEWLIRLVFKIEKEYSRELAYKINPANYQFGDRVVQLFSINDT
                     KIEEVNIKFTKVYQLIDELCCDKYVYFNCIIEKLVESYGEEYRDIATSYIMSLIDSHFL
                     ISNINSELIMNFKFEEFISKVKEIDKQNLYYFKLIAISNLIVEYSELEIGDGIEKLQEI
                     YKLMSSLVETPNFLQIDLYNDGQIQLGNENKTQIVEFAEFLVSNSDHVKRTYLDDYKEK
                     FLDKYGVNREVQLMELFDSNLGIGSPYGYQHPKNDFWESSPSTVYFSEKEELEYLNNFE
                     KALETGGNIQLYNEEDFFNQDKDKINLGFELFFYVNKVDGKSVLSLTNTGCSKNLGASS
                     GRFSILSDKLEHYHQTITQIVENNNHVSGYNSCEITFLPENLRHANVMRTTNVREKVLS
                     LFTNMDKRSQILSDIYIGIDSKNSFYARNFKTKELLKFYSTNMYNQMMFSNELRFLCEI
                     AQEDHFGIFPWEMVYQKFSHIPRIVFKDIIVAPERWRLSGRMLSKDIHQIILENNLPTK
                     LYVDNSDNRILINRNNPLDNQLFEDIVRKSSNKNEELCLSECIFDSHLVEKESTTHISD
                     IVVPVFAKDEIKEPRYIKEILPEVIPTNVRQKIPFDEWLYFKLYMSADRQEEFLSDIMP
                     QILKLVNLDDGMSFYIRYTDPKFHIRLRIRTQNLYKSFEKIQEIFQLCIQNKLISNIDI
                     STYDREIERYGGLERIPLVEEIFCLDTEIVINSLSLIRQKRLDLTLDDLAIIFNYFYLK
                     SFFKDNNKEIIQFLEFACPEHLDSQNRDKNRNTALIDLYLIKGYLINLLPELSKLCQRL
                     KKLSDSCIQLSDDYTYIIYDSIIHVHNNRLFGIKRENETKIYAIIRGLIISEEFRNGHN
                     HG"
     gene            9771..11579
                     /gene="sslT"
     CDS             9771..11579
                     /codon_start=1
                     /gene="sslT"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 166.8; E-value:
                     1.1e-50)"
                     /gene_kind="transport"
                     /product="ABC transporter"
                     /protein_id="AOT85870.1"
                     /transl_table=11
                     /translation="MGTIMDKFTLKNFFQTILTIPKTIRLIFRIERKYASYLIILNILQ
                     SIIPLASLFIYQELINAVLKKGSNIFSVLIVYILMQMMLSILSQLNSYISEKFNMNLSY
                     NLNLKLLKKTSSLNLIDFEQADTYNLIENIVQDSTYKPFQLFNAIIGVITGFLSLLTSI
                     IYVSTWNVAVSTFLLIIPVISLVIFMRVGQLEFLIQWERANSEREVWYINYLLTHDFSF
                     KEIKLNGISQYFIDKYGTLKRNFMNQDLNISKKKVLFRGSLDILLNFINGIAIFMMIQS
                     IRSGKLLVGNFVSLIQAITRVNTYSQSMIQNTYIIYNTSLFMEQLFKFFDMEVSNISIQ
                     NSRISRIEKRIDKIKVNNLSFIYPGSVKKTLEDINVEFNKGELVAIVGKNGSGKSTLVK
                     LLSGLYQPSEGQIYYNNESSDVLDLSYYQNNVSVLFQDFVKFELSVRENIGLSDVNSIS
                     DSKIKKHIDNLKLNFLTAENNFNLNQRLGTWFNDSRQISGGQWQKVALARTFFKNASIY
                     ILDEPSSALDPVSEKEIFDEFVTRSKDKIALFVSHNLMAASRADRIIVMQDGRIIDEGK
                     HDDLISKSKYYRELYYSEKYEEESDG"
     gene            11572..12849
                     /gene="sslC"
     CDS             11572..12849
                     /codon_start=1
                     /gene="sslC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140:Lanthionine synthetase C family protein (Score:
                     307.4; E-value: 2.3e-93)"
                     /gene_kind="biosynthetic"
                     /note="involved in lantibiotic synthesis"
                     /product="cyclase"
                     /protein_id="AOT85871.1"
                     /sec_met_domain="LANC_like (E-value: 3.4e-59, bitscore:
                     189.9, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDENKVIIDLSEKVFAKFDEQLKRYAEQPNYDLLTLSSGLPGLIL
                     LSSELTSLTSERKYSARTGKYVNFMVKQMRNYGVLSDSLFSGVSGIGISILHLVEEHPE
                     YHNLLISFNEYIKYYTLSKIENIDIKKISPTDYDIIEGVSGVLVYLLSQEQDENDYIIN
                     RIINFLSEFSLKNSTLTGFYVESKNQMSKTESKLYPLGCLNFGLAHGLAGVGAMLSYSK
                     LKGYSNEKSIAAIKKIIMLYEKYELKNYMWKEGLSDIELKKTEKSNLQYEFIRDAWCYG
                     SPGISLLYLYSSLALEDKKLKSKACNILKASIRRSNGLEQSILCHGFSGAIEICLFFKK
                     IYKTTDFDDCIKSLKEKLISDFREDMTYGFNTTAEFENIKTKDNLGYLDGIIGILLTMI
                     ELNNLKVTTNWQRALLLFDDVIKEVK"
     gene            12851..13567
                     /gene="sslI"
     CDS             12851..13567
                     /codon_start=1
                     /gene="sslI"
                     /product="immunity protein"
                     /protein_id="AOT85872.1"
                     /transl_table=11
                     /translation="MRKILFSLVVLIGICTLVACRLVDNKTIEFHDQSFNQFTYKGNDY
                     TIMNQIVDENDLGTVKESFFKTLVIDRNSQKVVTKSNSDAVSLAYSGLYNFSDGLAISI
                     NDSYYKVSLSSDVQSNNEMLSLEEFNNNSAGGELAIDPSDCRIVKFNNKKFRISSDIVS
                     DDKLKEFLGVIADSKTFILNTGQEISKSELNKIDYSGSNSNEKREVWDYGEVYLLAEEG
                     TIAVEINNEFRIARIE"
ORIGIN
        1 gatatagaga accgccgctt ggccaaatat gactatgcca agatgaatct aactgcggct
       61 ataaaactgg aacaagttga gaaagaaatc gaaatctatc aaattaagtt agataagtca
      121 atagatgatt atgagtattt aataaggagg ttggaaaagt ttgtcaaaat tttaaatgaa
      181 atagactcaa gcaaggatga actaaaattt gaacacagtg aaaaaaatgt gaaagaatag
      241 catttttcgg atttattgat accaaatatt gagtactctg aagaaattct gaaataaaat
      301 tgataaaaag gaataaaaaa ctaaggagat gatattatga aaaagatagg cttattgtcg
      361 aatgttttta tatcattgtt tattgttata ttagctagcg gttctgtgtc atcaggagta
      421 agtgctaatg aaaataagga aattagttat aatgttttag ttagtgaaaa taaatctatt
      481 gaggatgtag aagatgtttt acaaagtcaa ggaattaaag ttactgataa aatacctgaa
      541 ataaattttt taactatttc taccgaaaaa agtgaagata caataaaaaa agaaaactct
      601 aattatattg atgacatagt tatagataat actatgactg taaagcctaa tattacttat
      661 ttgtatgggg acaaggttta tacaaatact aatttagatt tttggggaaa tcagtgggat
      721 atgcaaaaga gtattagtac tggaagtaaa tttcaacata agtcttctgg taaagcaaca
      781 attggtgtaa tagattctgg tattacatat gataatccag atatttattc acacattatt
      841 tcagtacaaa atttcacatc agatttggaa acaggaatag ttgataacca aaatgttgta
      901 gataaaattg gccatgggac ctctgttgtg ggacaaattt catcaagtgg agaatattta
      961 gggatagcac ctgacatgaa aatacgaatg tatcgtgttt ttgatgaagg gaatgctcaa
     1021 gaccaatgga ttctcaaagc gctcattcaa gcagcaaaag atgatgttga tgttattaac
     1081 cttagtttag gagaatattt gttaagagat tccactgatg aagatgatga tacagctttg
     1141 atcaatatct atcaacgggc aattaattat gctcataatc aaggttccgt tgtcgttgct
     1201 tcagtaggtg atgaagggat ggatttacag aatcaagatg aattaaaaaa ctatcttggt
     1261 agtttaagag gaaaagatta tagtcaagtt gatggaactg tacaagatat tccggctgaa
     1321 ttagacaatg ttgttacagt tggttcagta gatgataatg atcttgtatc tagtttctca
     1381 aatcaaggga atggcgacat tgatatctat gcaacaggag gtggaagtca agcgctttct
     1441 aaagtaggat atgataaatg ggtagctgat aaattatatg aaaaagaatg gattcttgtt
     1501 ccaacgcttg aaggaaagta tacatatgca tatgggactt caatttcgac tcctaaagtc
     1561 tctgcagctt taggattgat tatagaaaag tataatttga aggaccaacc agatgaagct
     1621 attcgaattt tatatgataa ttgttttctt agtaataatg gaaacaatgg acaaattagg
     1681 ctactaaata ttacaaattt tgtgcaataa tagtgtaaaa tagtagtatg agataaaaga
     1741 ggtacctagt atgtataaga ttttggtagt tgatgatgat tttgaaattg taaaattgat
     1801 gcgaacaatt ttagaaatga aaaactataa tgtgacgact tatcaagaag ttaggcttcc
     1861 tataaaaata agtgattttg atggatttga tttaatattg cttgatgtta tgatgccaaa
     1921 tgttgatggt ctgcaaatct gtaaacagat tcgtaaatca gtatcctctc cgattatttt
     1981 tgtcagtgca aaagattctg aagatgatat tgtatctggc ttaaatctcg gtggtgacga
     2041 ttatataaca aagccattta gtgttaatca actagttgcc aaggttgcag cgcatttaaa
     2101 acgagaagaa cgttatagaa atgctgaatc taatgaatct gttattcgag aattacttcc
     2161 actaacattt taccttcaag aaaaactcgt gcgtgttaat ggtgaagcta tttctcttac
     2221 taaaagggaa tatgatattt tagagctatt atctagtaag acaaataagg tttatacaag
     2281 agaagaaatc tatgaatgtg tctatgatga tgaagcagat gcattatttc gttcaatttc
     2341 ggaatacatt tatcaaatta gagtcaagtt tggttcctat ggaattaatc ccattaaaac
     2401 aataaggggg atgggatata agtggaatga tgaaaagata ttcaattaat aagcggattt
     2461 taaggacagt tcttgaaata gtgattggta cagttgcaaa tgttattatg ttgcttgtat
     2521 tgagctacgt tcttattttt acaggtcagt tgaatacata tggaacagac tatcatattt
     2581 caattagtga tagtaaatct gctaacggta ttaagaatca aatggattac tctgtctttg
     2641 attatgtatt gtttgatagc gataatggcg aattggtctc tggaaaatat caaaaacagg
     2701 atttacaaca ttataaagaa gtttttgata ctggaagttc ggttagcgag ggttctatat
     2761 tgtttacaga atatgaaaac tcagatttta ttttggttat tagaagacca acagtgcccg
     2821 aatttgtaaa tcaaaacttt cgacatattt cctataattt agtctcttat ttgtttttta
     2881 tagtgctaga attactactc atctctatct ccgttatcag gctcttaaga gaatttacaa
     2941 aaaattttcg tttaattgaa aaaatatctc tgaatatggg gataccagga atggatattg
     3001 gacgtaaaga aactgaaatc ttagaatgta ataacatttt attgacgctt tatcaaaaag
     3061 acgaagagtt gacaaagtta ttagaaatgg agaaaaaaga taaagaagat ttgtcatttc
     3121 aagttgctgc attagcacat gatattaaaa caccattaac tgttttaaag gggaatatcg
     3181 agttgcttga gatgactaat ctagatgaaa aacaattaga ttttatgtcc tcaataaatc
     3241 atagtgttgg tatatttgaa aagtatttta attcaatggt taattactca aggttgctaa
     3301 ttgaggataa gaattatcag gaagatattg aattagcaaa atttcttgat gaattatcat
     3361 tagaatgtcg agatattatg tcttcaaaaa aaattaattt tcaaatagtt aatgaatccc
     3421 ataccagagt gattaaagga aatcgtctca acttagatcg agctttgata aatatcttat
     3481 ctaatgcgac tagattttgt tcagaagagg gcaaggtggt actgactata tctgaaagtt
     3541 ctaactttat cagttttgag atttggaata atggtttacc atttacagaa gagtctctta
     3601 aagatggagg aaaacttttt tacacagaga ataaaggaag aaatgataag cattacggaa
     3661 taggactttc atttgctcaa aagatagcta tgagacatca aggtcaatta atactctcaa
     3721 atccacaaaa tggtggtgca caggtcactt tattaattaa aaaaatttta cgggctggtc
     3781 aaaaagccta atataaacaa aaacgcatga aatctttcat ttacaaacgt tgattttatg
     3841 cgtttttttg tttcgaaaat ttgaaaaaat caatgagata tcgacttttt gctcaatctt
     3901 tttttctgaa caaatactga aatttctttt gtattataat aaaagaaatg gaggcagaaa
     3961 aaatggaagt tatcattcaa aatctaagaa aaagctataa agacaaagag gttttaaaag
     4021 acgtttcatt taaaattgaa gaaggtacta tttgtggtct tctgggagta aatggggctg
     4081 ggaaatccac cattatgaaa attctttttg ggttagaaag agctgatagt ggagaagtca
     4141 ttttcaatgg aaagcataag gacattgtag aagaaaatag atttgatatt ggagcgttga
     4201 ttgaatctcc tgctatctat ttgaatcttt ctgcatttga caatttaaaa acacgagcat
     4261 tgctacatga tattccagat gaaagaattt gtgaggtact tcagttggtt ggtttatctg
     4321 atactggaaa aaagaaggca gggcactttt cattaggaat gaagcaacga cttggtttag
     4381 gaatggcaat tattaccaat cctgatttat taattttaga tgagccaact aatggtctag
     4441 atccagatgg gataaaagaa ttgctaaatt taattaggga tctaaaaaaa tctggaatga
     4501 cagtcctctt gtccagccat caactaagtg aagtcagcaa aattgcggat caaatcgtta
     4561 tactgaatca gggcaagatt ttttacgatg atattaataa gaattcaaat gatttagaaa
     4621 aactattctt tagaattgtc catggaggtg atggaaatga ctaatatttt taaatctgag
     4681 tggctgaagc aaaaatctag ttctgataaa aaattactaa tccttattcc tttgctagca
     4741 atttttatag ctttcatact agcaggacct ctcaatttag aaagtttttc aatttattgg
     4801 tgggagggag gattcctttt cactctcctt gggttattgt ttttagctga ttatagcgcg
     4861 gaggagaatg caggtgcgtt tcaaaatatt agcttgggta gatatacgtt tactatttat
     4921 ttagcgaaga tactgttgaa gttcaaggat gttttgattt caacctttat ttttacgata
     4981 atattttatg gcatctcatt tcttttttca ggtgtgatat cagtagacat tggtaaagat
     5041 ttgatttgtt tattacttat tggtgtttct tctgcttggg tattaccact attatatttt
     5101 ctagcaaagt ggataaatcc ctacttgttg ttggctacaa attctcttat ttgcttatta
     5161 gttgctcctc taatagctca gacatcggtt tggttcatat ttccttatac ttatcactat
     5221 aaaattgctt atgctctttt acatttaaaa ccctcaggtg atttagaggc agcattaagt
     5281 ggagttgaaa tatcgacaat tttaatatca gtatctcttt caatcttagt ttttgtgagt
     5341 ttccttagtt tgttgaagtg gaggataatt aatgaacaaa attctaaaaa gtgagttgct
     5401 aaagttgaaa ggctctctta ctttaaatct tattttaatt ttatcaatta ttcaattatt
     5461 cacaattcct ctttatttac agtttacaaa taattcagtt gtaattgaaa atatcatttt
     5521 tttacctatg ttaggatatt gtatattagc atcaattttc tctatttttc ttcatgaaca
     5581 agaggaaaaa gcaaatttct ttcaaaatat aaaaagcgaa aaaaatagtg gaattatttg
     5641 gggaataaag cttatttcga cagacttact tatggtttta ttgggagttc cagtttggat
     5701 agtggtagga gttgaattta atagactctc ttactttgcg tatgttggtg tgattacttg
     5761 gttattatta gtcttactta accatttcca tatgttactt agtcttatca tgggaaaggg
     5821 agggaatcta gtaatttctt ttatcgaatg tctgtttatt atatttgcaa ccaataaagt
     5881 atttttaaat atcttctggt tgcctatcgt tttacctgtt aatatgatcc tagaaattgg
     5941 gaaaaatgaa atttttatga ttctagtata cttaattggt tttatcatat tatcgtattt
     6001 ttgtaattta gctgttatga ataatgttga aattcaaaaa atatgtaaaa agaggtaaat
     6061 tggttagagg tcactaaaag agtagaatac aacaaaattc aatagataaa aagcgaatga
     6121 cagcatattt tgaaatctaa agattgcagg tcatccgctt tttatgtcta ttttattatc
     6181 ttttcaagac agtattgttc atgatatttt aaaaatatca gactttttca gcagtttcat
     6241 tagtcaacta tctttttatt taattgtcca gagtatcagt ataaatattt catgcattct
     6301 aaataaaaaa atttgttcat tctctataat tatgaacaaa ttctgaacta ggtatcttac
     6361 aatatatttg ttcgaacaaa gaaaaaataa ttggaggaat tttattatga acaatgaaga
     6421 ttttaatttg gatcttgtca caatctcaaa ggaaaacaac tcaggagctt cacctcgggt
     6481 aactagtaaa tcattatgta ctcctggatg taagacgggt attttgatga cctgtgcaat
     6541 caaaactgca acttgtggtt gccattttgg ataaaattta tttgtttagt aactatcttt
     6601 ttgcaaatct tgactaagaa agaaagggac tctatatctt ttcgagtaag gtccctctaa
     6661 atattagaat atttgtttag gatattactc tttagacgaa tatgtctatt gctaaaataa
     6721 aggtagtgga tagtagtcta ctacctttat ttttataaat ttccaaaata atatttgaag
     6781 atttataaaa ataatttaga agttggagct gctaatggaa cataaatata ctacaaataa
     6841 ttttttggtt cgtaatgcaa tcattggaat acatgaattg ttggaatgtg attataattc
     6901 atttttagaa actataagag agaataagat attccaggag caactatttg tagccagcag
     6961 gagtttatat gaatcgcttc aaaaatatta tagtggtgat tcaatgaaaa ggaaaaaaat
     7021 caaccaatta tcagaatctg tatataaata ttataagcgt agcaaggagc gctcaacacc
     7081 atttggttta tttagtgaaa cttcaatagg ttcattttct tctacagaga aattaaactt
     7141 gaatggaaaa acattaaaaa aagtactctt ggattcagag tggttaatac gtctagtttt
     7201 taagattgag aaggaatatt ctcgagaatt agcctataaa ataaatccag ccaactatca
     7261 atttggagat agagttgttc aattgttttc tataaatgat actaaaattg aagaagtcaa
     7321 cataaagttc actaaagttt atcaacttat tgatgagctt tgttgtgata aatatgtata
     7381 ttttaactgt attatagaaa aacttgttga atcctatggt gaagagtatc gagatattgc
     7441 aacatcatat atcatgagtt tgattgatag tcattttcta atttcaaata ttaattcaga
     7501 attaataatg aattttaagt ttgaagaatt catttctaaa gtcaaagaaa tcgataagca
     7561 aaatctttat tattttaaac taatagcaat tagcaactta attgttgaat attctgaatt
     7621 ggaaattggg gatgggattg aaaagcttca agaaatctac aagttaatgt catcgttagt
     7681 tgaaacccct aattttcttc aaattgatct ttataatgat ggtcaaatcc aattaggtaa
     7741 tgaaaataaa actcagatag tagaatttgc tgagtttcta gtaagtaatt cagatcatgt
     7801 aaagcgtact tatttagatg attataaaga aaaattttta gataagtacg gagtaaatag
     7861 agaagttcag ttgatggagt tgtttgattc taatttagga attggttctc catatggata
     7921 tcagcacccc aaaaatgatt tctgggaatc ttcgccttct actgtatatt ttagtgaaaa
     7981 agaagaatta gaatatctca ataattttga aaaagcctta gaaacaggtg gaaatattca
     8041 actttataat gaagaagatt tttttaatca agacaaagat aaaatcaatc taggatttga
     8101 gttgttcttt tatgttaata aagtagatgg taaaagcgta ctaagcttaa ctaatacagg
     8161 atgttctaag aatttaggag ctagctcagg caggttttct attctatcag ataaattgga
     8221 acattatcat caaactatca ctcaaattgt tgaaaacaat aatcacgtat cagggtataa
     8281 ctcttgtgaa ataacatttt taccagagaa tcttcgtcat gctaatgtta tgcgcacaac
     8341 aaatgtcaga gaaaaagtat tatcactttt tacgaatatg gataagagaa gtcaaatttt
     8401 atctgatata tatattggta ttgattctaa gaatagtttt tatgctagaa atttcaaaac
     8461 taaggaactc ttaaaatttt atagtacaaa tatgtataac caaatgatgt ttagcaatga
     8521 attacggttt ttatgtgaga tagctcaaga agatcatttc ggaatattcc cttgggagat
     8581 ggtttatcaa aaattttctc atattcctag aatagtattt aaggacatta ttgttgctcc
     8641 tgaacgttgg aggcttagtg gaaggatgtt gtcgaaggat attcatcaga ttattcttga
     8701 aaacaatctt ccgacaaaat tgtatgttga taacagtgat aatcgaatat taattaaccg
     8761 taataatcca ctagataatc aactttttga agatattgta agaaaaagta gtaataaaaa
     8821 cgaagaatta tgtctatcag agtgtatttt tgattcacac cttgtagaga aggaatctac
     8881 aactcatatt tctgatatag ttgttccagt atttgctaag gatgaaatta aggaacctag
     8941 gtacataaaa gaaatattac cagaggttat tccgacaaat gttagacaga aaataccatt
     9001 tgatgaatgg ctctacttta aattgtatat gtcggcagat aggcaagagg aatttctttc
     9061 ggatataatg cctcaaattt tgaagttagt aaatttagat gatgggatga gcttctatat
     9121 cagatatact gatccaaaat ttcatatccg actaaggata agaacgcaga atttatataa
     9181 aagttttgaa aaaattcaag aaatttttca actctgcata caaaacaagt taatatcaaa
     9241 tatagatatt tcaacgtatg acagagaaat cgaaaggtat gggggactag aaagaattcc
     9301 tttggtagaa gaaatttttt gtctagatac tgaaatagtc atcaattcat taagtttaat
     9361 tcggcagaaa agattagatt taaccctaga tgatttagca ataatattta actactttta
     9421 tctcaaatca ttttttaaag ataacaataa agaaataata caatttttag aatttgcttg
     9481 tccagagcat ttagacagcc aaaatagaga taaaaatagg aatactgcat taattgattt
     9541 gtacttaata aagggatatt taataaatct tcttccagaa ctatctaaac tatgtcaaag
     9601 acttaagaaa ttatcagaca gttgtattca attatcagat gattatacat atatcattta
     9661 tgatagtatc attcatgtac acaataatcg cttatttggt ataaagagag aaaatgagac
     9721 taagatttat gctataattc gcggattgat aatcagtgaa gaatttagaa atgggcacaa
     9781 tcatggataa atttacacta aaaaattttt ttcaaacaat tttgacaatt cctaaaacaa
     9841 taagattaat ttttcgaata gaaagaaaat atgctagcta tctaataatt ttaaacatat
     9901 tacaatcaat aattccttta gcaagcttat ttatctatca agaattaatc aatgctgttt
     9961 taaaaaaagg aagtaacatt ttttcagtat taattgtcta tatattaatg caaatgatgc
    10021 tatcaatact aagtcagtta aactcatata taagtgagaa atttaatatg aacttgtcct
    10081 ataatctcaa tttgaagtta ttgaaaaaaa catcatctct aaatttaata gattttgaac
    10141 aagcagatac atacaatctc attgaaaata ttgtacaaga tagtacatat aagccgttcc
    10201 aacttttcaa tgctatcatt ggtgtgatta cagggtttct atctttatta acaagtatta
    10261 tttatgtgtc cacttggaat gtggcagtat cgactttttt actgataatt cctgttattt
    10321 ccttagtaat ttttatgcgt gttggccagc tagaattttt gattcaatgg gagcgagcaa
    10381 attcggagag ggaagtttgg tacatcaatt atcttcttac tcatgatttt tcatttaaag
    10441 agattaaact aaatggtatt agtcaatatt tcattgataa atatggaaca ttgaaaagaa
    10501 attttatgaa tcaggatttg aatatttcga aaaagaaagt tcttttcaga ggaagtttgg
    10561 atattttatt gaattttatt aatggtatag ctatttttat gatgatacag tcaattcgaa
    10621 gcggtaaact tttagtaggt aattttgtaa gccttattca agcaataact cgtgtaaata
    10681 cctactcaca atctatgatc caaaatactt atatcatcta taacacaagt ttattcatgg
    10741 aacaattatt taaatttttt gatatggaag taagtaatat ttcaattcag aatagtagaa
    10801 tctcaagaat tgaaaagcgc attgacaaaa taaaagtcaa taatctctct tttatctatc
    10861 caggaagtgt caaaaaaact ttagaggata ttaatgttga gtttaataag ggtgaacttg
    10921 tagcaattgt ggggaagaat ggttctggta agagtacctt agtaaaatta ctatcgggat
    10981 tataccaacc ttcagagggt caaatatatt ataataacga atctagtgat gtcctagact
    11041 tgagttatta tcaaaataat gtttctgttc tttttcagga ctttgtgaag tttgaattat
    11101 ctgtgagaga aaatattggc ctaagtgatg taaacagtat atctgactcc aaaataaaga
    11161 aacatattga taacttaaaa ttaaatttct taaccgcaga aaacaatttc aaccttaatc
    11221 aacgtttagg gacctggttt aacgatagta gacaaatttc aggaggacaa tggcaaaaag
    11281 tagcattggc taggactttc ttcaagaatg caagcatata cattttggat gaaccaagtt
    11341 cagcgcttga tcctgtttca gagaaagaaa tatttgatga atttgtaact cgttctaaag
    11401 ataagattgc tttgtttgtg tcacataatc taatggctgc gagtagagca gatcgaatta
    11461 tagttatgca agatggacga attattgatg aaggaaaaca tgacgacctt ataagtaaat
    11521 ctaaatatta tagggaactt tactattcag aaaagtacga ggaggagtct gatggatgaa
    11581 aataaagtta ttattgattt atctgagaaa gtatttgcta aatttgatga gcagttaaaa
    11641 agatatgctg aacaaccgaa ttatgattta ttgaccttgt cttctggttt acctggattg
    11701 atattattat cttcagaatt gacaagttta actagtgaaa gaaaatactc cgctagaacg
    11761 ggtaaatatg ttaactttat ggttaaacaa atgagaaatt atggtgtttt atcggattct
    11821 ttattttcag gagtttctgg aattggaata agtatacttc atttagttga agaacaccca
    11881 gaatatcata acttattgat tagttttaat gagtacatta agtattatac gttaagtaag
    11941 attgaaaata tagatattaa gaaaatctct cctacagatt atgacatcat agaaggagta
    12001 tctggagtat tagtttatct tttatctcag gagcaagatg aaaatgatta tataataaac
    12061 agaattatca atttcttatc tgagttttca ctaaaaaatt ctactttaac aggcttctat
    12121 gtggaatcaa agaatcaaat gtccaagaca gagtctaagc tttatccgtt aggatgtcta
    12181 aattttggtt tagcacacgg ccttgcgggt gttggcgcaa tgttgtctta ttcaaaatta
    12241 aaaggctatt ctaatgaaaa atctattgca gcaatcaaaa aaattataat gttatacgaa
    12301 aaatacgagt taaaaaatta tatgtggaaa gagggattaa gcgatattga actaaagaaa
    12361 acagaaaaaa gcaatttaca gtatgaattt attcgagatg cgtggtgcta cggtagccca
    12421 ggaattagtt tactatatct gtattcaagt ttggctctag aagataagaa actaaaatct
    12481 aaagcttgta acatcttgaa agcatccatc aggagaagta atggtttgga acaatctatt
    12541 ttatgtcacg gattttctgg agcaattgag atttgcctat tttttaaaaa aatttataaa
    12601 acaactgatt ttgatgactg tataaaatct cttaaggaaa agttaattag tgattttaga
    12661 gaagacatga catatggttt taatactacc gcggaatttg aaaatataaa aacaaaagat
    12721 aatcttggtt atttagatgg aataattgga attctgttaa caatgatcga actaaataac
    12781 ttaaaggtta caacaaattg gcaaagggca ctcttattat ttgatgatgt tataaaggag
    12841 gtgaagtagc atgaggaaaa ttttatttag cttagttgtt cttattggaa tttgtacttt
    12901 agttgcatgt agattggtag acaataaaac aatagagttt catgatcaat catttaatca
    12961 atttacatat aaaggaaatg attatacaat catgaatcaa attgttgatg aaaatgatct
    13021 aggtactgtg aaggaatctt tttttaaaac attagtaatt gatagaaata gccagaaagt
    13081 tgttactaaa agtaatagtg atgcagtatc attagcatat agtgggctct ataattttag
    13141 tgatggttta gctatttcta taaatgattc ttattataag gtttctttga gttcggatgt
    13201 gcaatccaat aatgaaatgc tgagcttgga agagttcaat aataattcag caggtgggga
    13261 acttgcaatt gatccatctg attgtaggat agttaaattt aataataaaa aatttcgtat
    13321 ttcatctgat attgtatctg atgataaact aaaagaattt ctaggagtaa tagcagattc
    13381 aaaaacattt attttgaata cgggacaaga aatttctaaa tcggaactaa ataaaataga
    13441 ttattctggt tctaattcta atgaaaaaag agaagtttgg gattacggag aagtttattt
    13501 gttagcagaa gaaggaacca tagctgttga gattaataat gaatttagaa tagcgcgcat
    13561 agaataaatt atagattatc taggtattac attgcttata tagcaaaata tcttgttgtt
    13621 acctactaaa cagaaactta ttaaaata
//
