LOCUS       BGC0001802             11484 bp    DNA     linear   BCT 16-OCT-2017
DEFINITION  Streptomyces malaysiense thioholgamide biosynthetic gene cluster,
            complete sequence.
ACCESSION   BGC0001802
VERSION     BGC0001802
KEYWORDS    .
SOURCE      Streptomyces malaysiense
  ORGANISM  Streptomyces malaysiense
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 16:19:40
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..11484
                     /db_xref="taxon:1428626"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces malaysiense"
                     /strain="MUSC 136 57"
     misc_feature    1..11484
                     /note="thioholgamide biosynthetic gene cluster"
     protocluster    1..11484
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[6102:8162]"
                     /cutoff="20000"
                     /detection_rule="((YcaO or TIGR03604) and TfuA)"
                     /neighbourhood="10000"
                     /product="thioamitides"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      6103..8162
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((YcaO or TIGR03604) and TfuA)"
                     /neighbourhood="10000"
                     /product="thioamitides"
                     /protocluster_number="1"
     protocluster    1..11484
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[1123:4238]"
                     /cutoff="20000"
                     /detection_rule="(APH and HopA1)"
                     /neighbourhood="20000"
                     /product="lanthipeptide-class-v"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      1124..4238
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(APH and HopA1)"
                     /neighbourhood="20000"
                     /product="lanthipeptide-class-v"
                     /protocluster_number="2"
     cand_cluster    1..11484
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(APH and HopA1)"
                     /detection_rules="((YcaO or TIGR03604) and TfuA)"
                     /kind="neighbouring"
                     /product="lanthipeptide-class-v"
                     /product="thioamitides"
                     /protoclusters="2"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..11484
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001802"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11484
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-v"
                     /product="thioamitides"
                     /region_number="1"
                     /rules="(APH and HopA1)"
                     /rules="((YcaO or TIGR03604) and TfuA)"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             855..1106
                     /codon_start=1
                     /note="ThoA"
                     /product="hypothetical protein"
                     /protein_id="ATJ00792.1"
                     /transl_table=11
                     /translation="MVSAVDETAFADLVSKIQDAEASMTDEQRAVIDPASGEKALQELA
                     GVSPEELQAFLEEKAGISPDEEAQGSVMAAAATVAFHC"
     CDS             1124..2293
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-v: APH"
                     /gene_kind="biosynthetic"
                     /note="ThoC"
                     /product="hypothetical protein"
                     /protein_id="ATJ00793.1"
                     /sec_met_domain="APH (E-value: 4.3e-15, bitscore: 45.8,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMTSASHSPAGVASPKTGREGGSMSSPWCGALPNESGPEKARTRS
                     WLSSLGIDRGGIVRIQTFAARNSVAMLHFEDGTALFLKQVDPATARAVGTATGNEEKML
                     RLIPESDVPHHVRTAVPRFIATDPETETLVMEGKTGFVSMRDVGRKFPEIPVPLLVSLA
                     SVTAGLHTSTVERFTTDPGYRPQLIDFPFASFVTLTPSELASGPGMDYSDYAATMQAVD
                     DSVARMREEWTPRRLVHFDLRDDNILVKDAEGARPQVALVDWELSGFGDPMLDVGTIIG
                     QFLIQWLHTVRGDAGRLADASTWATVRRNVGLFLTAYEHSAPLSLEQRDLAFRYAGLSV
                     LMYAAGRLEQIGSLGQIGHLCLFVGRTLLNDPRGVAELLAPGILRKAGA"
     CDS             2290..3294
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-v: HopA1"
                     /gene_kind="biosynthetic"
                     /note="ThoD"
                     /product="hypothetical protein"
                     /protein_id="ATJ00794.1"
                     /sec_met_domain="HopA1 (E-value: 1.7e-56, bitscore: 180.2,
                     seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNRYQAAFAAIAEDVEVLNESAFHHREWGELRPAPGVESVPEDLP
                     VLPHLSRFLYLSYYAGDTRAARWLIDGAPVVLGTRRREAPAVARALSEANQGSGYWDSD
                     WRTVTAGPERCRVRKNSLTLTVTAEEILPPSAGPGQPVSVRFPPERPYMYPGWYLAIGD
                     AGLPRHGERPVVRLYYAPRDPAAAAGLMRAITDLLCGAGVPYQLKAANHPEGYERRDAM
                     VLYLYRDDWRSQERALTDLHREHSGTLRDTGPAFALELGRGWWLADEPEQRHGRLMSFG
                     QHRCLLVAEGLVAAWRDGRTTAADRLGAIEERYRAERLDPAKPYLNAQGSTAR"
     CDS             3291..4238
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-v: APH"
                     /gene_kind="biosynthetic"
                     /note="ThoE"
                     /product="hypothetical protein"
                     /protein_id="ATJ00795.1"
                     /sec_met_domain="APH (E-value: 2.9e-08, bitscore: 23.4,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHTTVQAGQPVRSLPGPFVTRVCLRADGGYRWGESIPEATPAYL
                     DADALNALADAITPTPAARYACLPQLADARTAIHPVVATPALLSDAVFSGSAPNEAADP
                     NDVQVFRELGIFLAHLHAIPVAQVRALPVRPRPAWLDAAPDAAAGIRAARARLDQNAAP
                     RIRCLADTDSVPGYACPQAVVHGRLSIGSCVPGTVPRVLGWREAGIADPMTDLAFLLRD
                     LVQAAAAMGSRKLQERRAEQTIRGYETTRGVQLSKDEHARLATHLAAGILQHVALRAWS
                     ASDQQGAEALLRQGELALPGVLHAVGTPGEVIYR"
     gene            4235..4840
                     /gene="thoF"
     CDS             4235..4840
                     /codon_start=1
                     /gene="thoF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Flavoprotein"
                     /gene_kind="biosynthetic-additional"
                     /product="putative flavoprotein decarboxylase"
                     /protein_id="ATJ00796.1"
                     /sec_met_domain="Flavoprotein (E-value: 7.5e-30, bitscore:
                     93.1, seeds: 143, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSERASGPQEALAEVRLLWGVCGSFSAVAVPHVNAWLRGTVGIRE
                     IRTIMTAQAHALMGPRMIEAVTGHVPVTDWEDHTGGGAAHVALGSWADVLVVLPATANF
                     LAKAAHGIADDVLTATLLAAECPVIIAPVMNAAMWSKPAVRRNVDQLREDGYQIVEPKE
                     GISLTEGRREAGSLGDFQLALSAALVQAAARLTHPRGQ"
     gene            4848..6065
                     /gene="thoG"
     CDS             4848..6065
                     /codon_start=1
                     /gene="thoG"
                     /product="putative methyltransferase"
                     /protein_id="ATJ00797.1"
                     /transl_table=11
                     /translation="MPKLTPETVLHRTPSFIAEVDTSNEVKIHFESRILKVGHDALGLL
                     DIFHQPRTVGQALTLLGPRLKGPRAAQEALTTVVSLHQAGLLRTDATAGFSNQPFPKGG
                     YDAAFVHIAMLDDPVRKAAFQQAVRETVQEGDVVLDLGTGSGILAITAAQAGARKVYAV
                     EPAGMVHLARRIAERNGVADRIEFIPGWSPQLELPEQADVLLTDIVGNEALDMQIWETV
                     QDARRRLMTDAPRLVPGRLTSFATLARIPDGVLDKHRVTQAHLARWKEMFGIDFGPMAE
                     SDSRRSIGFYERPETVQTWEFLSKPTELYQVDLYQDMASPLETRCTLTADRAGEANAVV
                     IHFEARLGPNTTLSTAPWLGCARSHWYTAVWALPEPVEITTGQELAYIYRYFGDGTSHL
                     EPVGSR"
     misc_feature    5586..5588
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     CDS             6103..7455
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thioamitides: YcaO"
                     /gene_kind="biosynthetic"
                     /note="ThoH"
                     /product="hypothetical protein"
                     /protein_id="ATJ00798.1"
                     /sec_met_domain="YcaO (E-value: 5.5e-73, bitscore: 236.0,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLRSSVPKITGNGPHREITAETTYARMAPHLKRIGVTRVADITG
                     LDRVGIPVYNAIAPRSFDIISVYNGKGATKIDAKTSAVMEAAERFAAALPLRPAAIASY
                     PELEKSGRIVLDPRSCNFELHHAYRIDTPISWVEGYDLMHERNILVPQFLAGYYMSYHE
                     VPCYPVSTTNGIASGNSLEEAICHALCELVERDDWTMADLISNRLGKLAADDEGGPVGD
                     AIRAWAQERHPSLDASTLPPRAQFFMEKFLDAGISIEVKNITSATGIPSFQAVVSEYVA
                     DSFSRSHHGIGTHPDAEVAVVRAISEVAQSRVVDIQAMREDITAAGQPVEKALYHVRRS
                     NRANPDSWAIKPSARPIRFQDVPSHPSTDVMDDVRLLLQRLRARGLDQAIAVDLSAPGV
                     PATVVRVIVPGIESWTVDRNKLGPRATQAWNNALAAINSARQQLRTAGSAQ"
     gene            7470..8162
                     /gene="thoI"
     CDS             7470..8162
                     /codon_start=1
                     /gene="thoI"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thioamitides: TfuA"
                     /gene_kind="biosynthetic"
                     /product="TfuA-like core domain-containing protein"
                     /protein_id="ATJ00799.1"
                     /sec_met_domain="TfuA (E-value: 6.2e-45, bitscore: 141.4,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTVVFVGPSLPPEEAAAHLPGAEFLPPIERGDIDALMAQEQPPR
                     RIGIIDGKFLQSFSISPKEILRAMDRGVRVYGSSSMGALRAAELDVYGMTGVGTVYSMY
                     ASGALEDDDEVAITFDSESLRPVCEPMVNIRVALDAALRRRVVSKDNAELFLESAKALY
                     FPQRTAAAALHQLKGTMPEPDRQRLASYLRSPEAPDAKREDALALLDRMRQDAKQYKTV
                     PVPEVAAR"
     gene            8159..9031
                     /gene="thoJ"
     CDS             8159..9031
                     /codon_start=1
                     /gene="thoJ"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /product="putative phytanoyl-CoA dioxygenase family
                     protein"
                     /protein_id="ATJ00800.1"
                     /sec_met_domain="PhyH (E-value: 2.9e-27, bitscore: 85.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTAPDLAERFRTDGFVFPVNALTHAEAEQALAECQTYLRAVSA
                     VGGALARYAAFPKIHLVASWADRIVHHPAILDAVASLLGPDLLVWSTNLFIRPAYSGSS
                     LAWHQDAVYLGLDGYQQHAARVWVALTDTTIANGTMRYARGSHLHGALPHRFGGSGLED
                     IMRGEEIAVDIDEAAAVDVLLDAGQCSVHHLAMAHASGPNQTDTGRFNFAIDYITPRVS
                     PTAGEDSALLVRGTDTGAFLPERRPESDFDQAALNDFYSAVTRRQKRINQTVQNRNAGK
                     GPDTPRDPS"
     gene            9028..9861
                     /gene="thoK"
     CDS             9028..9861
                     /codon_start=1
                     /gene="thoK"
                     /product="putative protease"
                     /protein_id="ATJ00801.1"
                     /transl_table=11
                     /translation="MTTARPAGKESDSTVSEAADADWLSGIAPPWWTAGSVPHRMDLSG
                     WMPPVIDQGQVPLCTAAVTTAIAGYYALRAKQMEFTPSVLFNYRLSRRLAGSADRKGSR
                     LEHSFRAWAESGLCEEAAWPYDELGPTRVDRDPPEHCHVTARHSRPIARPLRAPDGAGL
                     LNLARRSIALGIPVSVEIRLCPSISMSLLNGGVIPVQLPTEQSVGPHVVLLTGYDDEAD
                     TAPYDRGTGSGAFRVRNSWGTGWGVQGYGLLPYAFLEQKLTGENWIVVEEDWQKQ"
     CDS             10090..10845
                     /codon_start=1
                     /note="ThoL"
                     /product="hypothetical protein"
                     /protein_id="ATJ00802.1"
                     /transl_table=11
                     /translation="MFSLGEAVAVGLLLELLLAGIVITEFTLATRAYKTSRSRGADGQE
                     ALNKALSTVLPAPVAKVMGMEFGLFRALWRWIRRKPDAPEGHQILTYGSEIKPIMWAMV
                     FLVPLEILAVEFLVPWSVVRIILLALSIYGTLWVLGFIAALSIRPHSVGDGKMVLRFAH
                     FVQIVIPLDLVESVRTTRHSGYRRAVQIDDGVLAMPIGDSTTLSVKLREPYPVAPKTGM
                     PLQEVREVRFAADNTREAVRILTANIVAD"
     misc_feature    10126..10128
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 gaggtgggca aagccgtgct cgaccggggc gcgggccgtg tgaggaccga agcgtctttc
       61 agacagtccc gctcgtgggc ggagaatgtg gcatcgtcag cctcttcacc gaccttgagc
      121 cccttccatc ttccggtgcc ggatgcttgg ctctgttgca gtgtcccggc cggaacagcc
      181 cagcgagtcg tgttggcatg aagcagtgtg cccgtttctg cattgatcaa gccatcatcg
      241 gtgagtgtcc gcaatagcat tgccgccgca tatcacgcct ctcaccaggg acaaagacgc
      301 cgtcgcggtg gtccacatct cgccatggca ccgatcagat gggtttcgac gcccgaaatc
      361 aagatggcca aatcctcaac cagaagtccc ctcgatcagc tgcgtgccgt ttccgccaaa
      421 tgggatactc gcaagccagc aatgaccgag gatcgcacct ccggaacgtc ttgatctttt
      481 cggccgacca ggcagcccct ggccacagcc gccgacatat gcctaccctc gccacgacca
      541 cgagattggc cacgggggct tctgatgact cactcgtcat gcacgcgcgc cagctcctag
      601 attcccgcac ccttgtaacc ctcacgacgt cgaatctccg cgcgcccact tagctagaca
      661 gctccacaat cgcctgccgg aaaatcgccg acaggctcat tcgcatgccc gaatttgaat
      721 tcccaacaca cacaagcgga atactggaaa cccattgaca gaggagggtg aacgtttgtt
      781 tagatcacat ccgtccattc gattcccccg ccgactggca ggggccgaat caggaggatc
      841 acatgtctga agcaatggtt tccgcggtgg acgagaccgc gttcgccgac ctcgtcagca
      901 agatccagga cgccgaggcg tcgatgaccg acgagcagcg cgcggtcatc gaccccgcct
      961 ccggcgagaa ggccctgcag gagctcgcgg gcgtcagccc ggaggagctg caggcgttcc
     1021 tggaggagaa ggccggcatc tcgcccgacg aggaggctca gggcagcgtg atggccgctg
     1081 ccgcgacggt ggccttccac tgttgatctg agtcgttcgt ctcatgatga caagcgcttc
     1141 gcacagccca gccggtgtcg cgtcgccgaa gaccggacgg gagggcggca gcatgtcttc
     1201 gccttggtgc ggggcgctgc cgaacgagtc cgggcccgaa aaggcccgga ctcgttcctg
     1261 gctctcttcg ctcggtatcg accggggcgg catcgttcgg atacagacgt tcgcagctcg
     1321 caacagcgtg gcaatgctgc acttcgagga cgggacggca ctcttcctga aacaggttga
     1381 tccagcaacg gccagagccg tcggcacggc aaccggcaac gaagagaaaa tgctcagact
     1441 gatacccgaa tccgacgttc cacatcacgt caggacggct gttcctcgtt tcattgcaac
     1501 cgacccggaa accgaaacac tcgtcatgga gggaaagacc ggcttcgttt cgatgcggga
     1561 tgtcggccgg aaattccccg agatccctgt accactactg gtgtcgctgg cctccgtgac
     1621 cgccgggctt cacacctcca cggtcgagcg cttcaccacg gacccggggt accgtccgca
     1681 actcatcgat tttcccttcg cttccttcgt caccctgacc ccgagcgagc tcgcgagcgg
     1741 accgggaatg gattactccg actacgcggc caccatgcag gcggtcgacg acagtgtggc
     1801 ccggatgcgc gaggagtgga ccccgaggcg gctggtgcac ttcgacctcc gggacgacaa
     1861 catcctggtc aaggacgccg agggtgcgcg cccccaggtc gcgctcgtcg actgggaact
     1921 gtcgggcttc ggcgacccga tgctcgacgt gggcaccatc atcggacagt tcctgatcca
     1981 gtggctccac acggtgcggg gcgacgccgg ccgcctggcc gacgccagca cgtgggccac
     2041 cgtgcgacgc aacgtcggcc ttttcctcac cgcatacgaa cacagcgccc cgctctcgct
     2101 ggagcagcgg gacctcgcgt tccgctacgc cggactgtcc gtcctgatgt acgcggccgg
     2161 gcggttggag cagatcggct cactagggca gatcgggcat ctgtgcctct tcgtgggccg
     2221 cacgttgctg aacgaccccc gaggcgtggc cgagctactc gctcccggca tcctccggaa
     2281 ggcaggcgca tgaaccgata ccaggcggcg ttcgccgcga tcgccgagga cgtggaggtg
     2341 ctgaacgagt cagccttcca ccatcgcgag tggggagagc ttcgccccgc ccctggcgtc
     2401 gagtccgtgc ccgaggatct ccccgtcctg ccccacctgt cccggttcct gtacctgagc
     2461 tactacgcgg gcgacacccg cgcggcacgc tggctcatcg acggcgcacc cgtggtgctg
     2521 ggcacgcgca ggcgggaggc cccagccgtg gcccgtgccc tgtccgaagc aaatcagggc
     2581 agtgggtatt gggactctga ctggcgcacg gtcacggcgg gaccggagag gtgccgcgtg
     2641 cgcaagaaca gcctgaccct gaccgtcacc gcggaggaga tactgccgcc gtcggccgga
     2701 ccggggcaac cggtgagcgt caggtttccg cccgagcgcc cgtacatgta ccccggctgg
     2761 tacctcgcca tcggtgacgc gggactgccc cgccacggcg aacgtcccgt cgtacgcctg
     2821 tactacgcgc ctcgggaccc ggcggcggcc gccggcctga tgcgtgcgat caccgacctg
     2881 ttgtgcggcg cgggtgtccc gtaccagttg aaggcggcca accacccgga agggtacgag
     2941 cggcgggacg caatggtcct ctatctgtac cgggacgact ggcgttcgca agagcgcgcg
     3001 ttgacggacc tgcaccggga gcacagcggc acgctccggg acaccggccc cgcattcgcg
     3061 ctggagctgg gccggggctg gtggctggcg gacgagcccg agcagcggca cggccgactg
     3121 atgagcttcg gtcagcatcg ttgcctgctg gtcgctgagg ggctcgtggc ggcctggcgg
     3181 gacggccgga cgaccgcggc ggaccggctc ggcgccatcg aggagcgcta ccgggccgag
     3241 cgactcgatc ccgcgaagcc gtacctcaac gcgcaaggga gcaccgctcg atgactcaca
     3301 ccactgtgca ggccgggcag ccggtgcgct cgcttcccgg cccgttcgtc acccgcgtct
     3361 gtcttcgggc cgacggtggg taccgctggg gagagagcat cccggaggcc acacccgcct
     3421 atctcgacgc cgacgcgctg aacgctctcg ccgacgcgat caccccgacg ccagccgccc
     3481 ggtacgcctg ccttccgcaa ctggccgacg cccgtaccgc catccacccc gttgtggcca
     3541 caccggcgct gctcagcgac gccgtcttca gcggctcggc gcccaatgag gcagccgacc
     3601 caaacgacgt gcaggtattc cgcgagctcg gcatcttcct tgcgcacctg cacgccatcc
     3661 ccgtcgcaca ggtgcgcgcc ctgccggtca ggccccgccc ggcctggttg gacgcggcac
     3721 cggacgcagc agcgggcatc cgcgccgcgc gcgcccggct ggaccaaaac gcagctccac
     3781 gcatccggtg tctcgccgac acggattcag taccggggta tgcctgtcca caggccgtcg
     3841 tgcacggtcg gctgtcaatc ggctcctgcg tccccggcac cgttccgcgc gtgctcggct
     3901 ggcgggaggc cggcatcgcc gaccccatga ccgacctcgc gttcctgctg cgggacctgg
     3961 tgcaggccgc cgcggccatg ggctcgcgga aactccagga acggcgggcc gaacagacga
     4021 tcaggggcta cgagacgacc cggggggttc agctgagcaa ggacgagcat gcacggctgg
     4081 caacacacct cgccgctggc atcctccagc acgtggccct acgcgcctgg tcagcgtccg
     4141 atcagcaggg cgccgaggca ctgctgcggc agggtgaact ggcccttcca ggcgtcctgc
     4201 atgccgtggg cactcccgga gaggtgatct accgatgagc gagcgcgcca gcggcccaca
     4261 ggaagcgctc gccgaggtgc ggttgctgtg gggggtgtgc ggctccttct ccgccgtcgc
     4321 ggtcccccat gtgaacgcat ggctgcgcgg caccgtggga atccgggaga tccgcaccat
     4381 catgaccgcg caggcgcacg ccctcatggg gccgcgcatg atcgaggccg tcaccggcca
     4441 cgtcccggtg accgactggg aggaccacac cggcggcggc gccgcgcacg ttgccctcgg
     4501 ttcctgggcg gatgtgctgg tggtgctgcc ggccaccgcg aacttcctgg cgaaggccgc
     4561 gcacggcatc gccgatgatg tcctgacggc gaccctcctc gccgccgagt gccccgtaat
     4621 catcgcgccc gtcatgaacg cagccatgtg gtccaagccc gccgtacgcc gcaacgtcga
     4681 tcaactacgc gaggacggct accagatcgt cgagccgaag gaaggcatct ccctcaccga
     4741 ggggcgccgg gaagccggtt cgctcggcga cttccagctg gccctttccg ccgctctggt
     4801 gcaggcggcc gcccgactca cacacccacg aggacagtga tcagcagatg cccaaactca
     4861 cacccgaaac cgtgctccac aggacgccgt ccttcatcgc agaggtcgac accagcaacg
     4921 aggtgaagat ccacttcgag agccggatcc tcaaggtcgg ccatgacgca ctcggcctcc
     4981 tcgacatctt tcaccagccc cgcacggttg gccaggcgct gaccctgctc ggcccccggc
     5041 tgaagggacc ccgggccgct caggaagcgc tgaccaccgt ggtctcgctc caccaagcgg
     5101 gcctgctgcg caccgatgcg accgcaggat tcagcaacca gccgttcccc aaaggcggct
     5161 acgacgccgc gttcgtccac atcgccatgc tggacgaccc agtacgcaag gccgctttcc
     5221 aacaggccgt ccgcgagacg gtccaggagg gggatgtcgt cctcgacctt gggaccggct
     5281 ccggcatcct ggcaatcacc gccgcgcagg cgggagcccg caaggtctac gcggtcgagc
     5341 cagccggcat ggtccatctg gcccggcgga tcgccgagcg caacggggta gccgaccgca
     5401 tcgagttcat cccaggctgg tcgccgcagc tcgaactccc cgagcaggcc gatgtcctgc
     5461 tgacggacat cgtcggcaac gaagcactgg acatgcagat ctgggagacg gtccaggacg
     5521 cccggcgccg gctgatgacg gacgcccctc gcctggtccc cggccgtctc acctcgttcg
     5581 caacattagc ccggattccc gacggcgttc tcgacaagca ccgggtcacc caggcccatc
     5641 tcgctcgctg gaaggagatg ttcggcatcg acttcggccc catggccgag tcagacagcc
     5701 gacgctccat cggcttctac gagcgcccgg agaccgtgca gacctgggag ttcctgtcca
     5761 agccgaccga gctgtaccag gtcgacctgt accaggacat ggccagcccg cttgagaccc
     5821 ggtgcacgct caccgccgat cgcgcgggcg aggccaacgc cgtcgtcatc cacttcgagg
     5881 ctcgcctcgg gccgaacacc accctgtcga cggctccttg gctgggctgc gcccgtagcc
     5941 actggtacac cgccgtctgg gcgcttcccg aacccgtgga aatcaccacc ggtcaggaac
     6001 tggcctacat ctaccgctac ttcggcgatg gcacatccca cctcgaaccc gtcggcagcc
     6061 gctgacctac ccggtgtaca gcgcaaccaa ggagcccttg ctttgaaact gcgttccagc
     6121 gttcccaaga tcaccggaaa cggcccgcac cgcgagatca ccgccgagac cacctacgca
     6181 cgcatggccc cgcacctgaa gcggatcggc gtgaccaggg tcgccgacat caccggactc
     6241 gaccgggtcg gtatccccgt ctacaacgcc atcgccccac gttcgttcga catcatctct
     6301 gtctacaacg gcaagggtgc gacgaagatc gacgccaaga cgtcggccgt catggaggcc
     6361 gccgaacggt tcgccgccgc cctgcccctc cggccggccg ccatcgcctc ctacccggaa
     6421 ctcgaaaagt ccggacgcat cgtacttgac ccccggtcgt gcaacttcga gctgcaccac
     6481 gcctaccgca tcgacacccc catctcctgg gtggagggct atgacctgat gcacgagcgc
     6541 aatatcctag tgccgcagtt cctggccggc tactacatga gctaccacga ggtcccctgc
     6601 taccccgtct ccacgaccaa cggcatcgcc tccggtaact ccctggaaga ggccatctgc
     6661 cacgccctgt gcgaactcgt cgagcgcgac gactggacca tggccgacct catctccaac
     6721 cgcctcggca agctggccgc cgacgacgaa ggcggtcccg tcggggacgc cattcgtgcc
     6781 tgggcgcagg agcgccaccc cagcctcgat gcgtcgacgc ttccgccacg ggcacagttc
     6841 ttcatggaga agttcctgga cgccgggatc tccatcgagg tgaagaacat cacctcggcg
     6901 acgggcatcc cgagcttcca agcagtggtg tcggaatacg tcgctgactc cttctcccgc
     6961 tcccaccatg gcatcggaac tcacccggac gccgaggtcg ccgtcgtacg tgctatcagc
     7021 gaggtcgccc agtcccgcgt ggtggacatc caggccatgc gcgaggacat caccgccgcc
     7081 gggcagcccg tcgagaaggc gctttaccac gtgcggcgca gcaaccgcgc gaatcccgac
     7141 tcctgggcca tcaagccgtc cgcacgcccc attcgcttcc aggacgtccc gtcccatccc
     7201 agcacggacg tcatggacga cgtacggctg ctgctccagc gcctgcgggc ccgcggcctc
     7261 gaccaggcca ttgccgtcga cctgtcagcg cccggtgtgc ccgccacagt cgtacgggtc
     7321 atcgtgcccg gcatcgagtc ctggactgtc gaccgcaaca agctcggtcc tcgggccact
     7381 caggcgtgga acaacgccct agccgccatc aactcggccc gacagcagct gcggaccgcc
     7441 ggttccgccc agtgagggaa gaagacatca tgcgtacggt cgtgttcgtc ggccccagcc
     7501 tgccgcccga ggaggctgcg gcccacctcc ccggcgccga gttcctgcca cccatcgagc
     7561 gcggcgatat cgacgcactc atggcccagg agcagccacc gcgccgcatc ggcatcatcg
     7621 acggaaagtt cctgcagagc ttctccatct cccccaagga gatcctgcgg gccatggacc
     7681 gcggggtccg cgtctacggc tcgtccagca tgggcgccct ccgggccgcc gaactcgacg
     7741 tgtacggcat gaccggagtc ggaaccgtgt acagcatgta cgcctccggc gccctggagg
     7801 atgacgacga ggtcgccatc accttcgaca gcgagtcgct gcgtccggtg tgcgaaccca
     7861 tggtcaacat ccgcgtcgcg ctcgacgcgg cgctgcgccg gagggtggtg agcaaggaca
     7921 acgcggaact gttcctggag agcgccaagg ccctgtactt cccgcagcgc accgccgccg
     7981 ccgccctgca ccagctgaag ggcacaatgc ccgaacccga ccgacagcgc cttgccagct
     8041 acctgcggtc ccccgaggct ccggatgcca agcgggaaga cgcgctcgcc ctgctggacc
     8101 ggatgcggca ggacgccaag cagtacaaga ccgtgcccgt cccggaggta gctgcccgat
     8161 gaccacgacc gctcccgacc tcgcggaacg gttccgcact gacgggttcg tcttccccgt
     8221 gaacgcgctc acgcatgccg aggccgaaca ggcgctggcc gagtgccaga cctacttgcg
     8281 tgcggtgagc gcagtgggcg gcgccctggc acggtacgcg gccttcccca agatccacct
     8341 ggtcgcctcc tgggccgatc ggatcgtgca ccaccccgcg atcctcgacg ccgtcgcgtc
     8401 actgctggga cccgatctgt tggtgtggtc caccaaccta ttcatcaggc ccgcttacag
     8461 cggcagctca ctcgcctggc accaggacgc cgtctacctc ggactggacg gctaccagca
     8521 gcacgcggca cgcgtgtggg tcgccctcac cgacaccacg atcgccaacg gcaccatgcg
     8581 ctacgcgcgg ggctcgcacc tgcacggcgc gttgccgcac cggttcggcg ggtccgggct
     8641 ggaggacatc atgcgcggtg aggagatcgc cgtcgacatc gacgaggcgg cggctgtcga
     8701 cgttctcctt gacgccggcc agtgctccgt gcaccacctg gccatggccc acgcctcggg
     8761 ccccaaccag accgatactg gacgtttcaa cttcgcaatc gactacatca caccgcgagt
     8821 ctctcccacc gcgggcgagg acagcgccct gctcgtccgc ggaacggaca ccggtgcttt
     8881 cctgcccgag cgcaggcccg agtctgactt cgaccaggcc gccctgaacg atttctactc
     8941 cgctgtcact cgtcgccaga agcggatcaa ccagacggtc cagaaccgga acgccgggaa
     9001 aggccctgac acaccccggg acccgtcgtg acgacggcac gccctgccgg caaggaatcc
     9061 gactccacgg tctccgaggc cgccgacgcc gactggctgt ccgggatcgc gcctccctgg
     9121 tggacggccg gaagcgtccc tcaccggatg gacctgtccg gctggatgcc gcccgtgatc
     9181 gaccaggggc aggtcccact gtgcaccgcc gcggtcacca cggcgatcgc cggctattac
     9241 gccctccggg ccaagcagat ggagttcacc ccctcagtcc ttttcaatta ccggctctcg
     9301 cgccgactcg ccggttccgc ggatcgtaag ggctcccgtc tggaacacag cttccgtgcc
     9361 tgggcggagt cggggctgtg cgaggaggca gcctggccgt acgacgaact cggtccgacc
     9421 cgggtcgacc gggatccgcc cgagcactgc cacgtcaccg cgcggcactc ccgcccgatc
     9481 gcgcgtccgc tgcgcgcgcc ggacggggcc ggcctgctca acctagcgcg ccggtccatc
     9541 gcgctgggca tccctgtgag cgtggagatc cggctctgcc cgtcgatctc catgtccctg
     9601 ctgaacggcg gtgtcatccc agtacagctg cccacggagc agtcggtggg acctcatgtg
     9661 gtgctgttga ccggctacga cgacgaggcg gacaccgctc cctacgaccg gggtaccggc
     9721 tcgggagcct tccgcgtccg gaacagctgg ggaaccggct ggggggtcca gggctacggc
     9781 ctccttccct acgccttcct cgaacagaag ctcactggcg agaactggat cgtcgtggaa
     9841 gaggactggc agaaacagtg agtgcggctc gtccggaaag ctctcttttt cggcccgcgt
     9901 ctacgaaaga aagcagccag tacgggtctt cgcgtcgctt agccacgaag agccagcggg
     9961 aataataacc agtgaaccta acacccccca caggaaagtt ggttgacgtt gcggaggttc
    10021 ctggcgacca ccgcccgttg ggcggcacct accgttgtcc tcgctcaggt ctgcctcgta
    10081 tggaccggcg tgttcagcct cggcgaggcg gtggcggtcg gactgttact cgaactcctc
    10141 ctggccggga tcgtgatcac cgaatttacc cttgccacgc gggcctacaa gacctcccgg
    10201 agccgggggg ccgacgggca ggaggccctc aacaaggcgc tatccaccgt gctccctgcc
    10261 ccggtggcca aggtgatggg gatggaattc ggccttttcc gcgctctttg gcgctggatc
    10321 cgccgcaagc cggacgcacc ggaaggccac cagatcctga cctacggctc ggaaatcaag
    10381 ccaatcatgt gggcaatggt gtttctcgtg cctctggaga tcctcgccgt cgagttcctc
    10441 gtcccctggt ccgtggtgcg catcatcctg ctggcgctct ccatctatgg aaccctctgg
    10501 gtgctcggct tcatcgcggc actgtccatc cgcccgcaca gcgtcggcga cggcaaaatg
    10561 gtgctgcggt ttgctcactt cgtacagatc gtgatcccgc ttgacctcgt cgaatcggtc
    10621 cgcacgaccc ggcacagcgg ctatcgcagg gccgtccaga tcgacgacgg cgtcctggcg
    10681 atgccgatcg gcgactccac cacgctcagc gtcaagctgc gcgagccgta ccctgtggca
    10741 cccaagactg gcatgccact tcaggaagta cgagaggtcc gcttcgccgc ggacaacact
    10801 cgtgaagccg tccgcatcct tacggcgaac atcgtcgccg actgatccgg ttgtcgaccc
    10861 tcgacggtca gggtcgccga gggcacatcc agggcacatg agcccgggaa acggcgttga
    10921 cctgtgagaa ctaccgagac gagttcccgc aggtcaacgc acacttcacc gagaaacccc
    10981 aggtcagcgc cccgccgccc ccaatcgctg cccgagtggc aggacttcag ccatcggttg
    11041 catgaggcgg ggcatcaggc ttattgcttg gtttggccct gagctgggtt tcttgcggtt
    11101 tgggcggggc cttcgggtcc cgctctcgtc atgtgccggg aggggtcgtc aggtcgcagt
    11161 gagggcacat gcgtcagggc tggagcactg atgtgccctg agcttcgccg gtgtccttgg
    11221 gggctccgcc tcggtgaagg cgtaatcgca gcaagccggg tcaggtgtca cccaaccctg
    11281 cagcagtccc aatcaggcaa cgctgccgag gccgtcagct tgctcgaagc gctgctaccc
    11341 gaccagacac gagcgctcgg gcccaagtcc cggtgccggg catccccccg ccgaaagtgc
    11401 tccgacaacg cgtcgtcgcc gacacgctca cgaccgccat ccaccccagc agcctgcccg
    11461 gcggctggtc cgtaacccgg tggt
//
