LOCUS       BGC0001808             10410 bp    DNA     linear   BCT 14-JAN-2017
DEFINITION  Streptomyces diastatochromogenes toyocamycin biosynthesis gene
            cluster, complete sequence.
ACCESSION   BGC0001808
VERSION     BGC0001808
KEYWORDS    .
SOURCE      Streptomyces diastatochromogenes
  ORGANISM  Streptomyces diastatochromogenes
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 16:20:05
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 10410
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            1..2877
                     /gene="toyA"
     CDS             1..2877
                     /codon_start=1
                     /gene="toyA"
                     /gene_functions="regulatory (smcogs) SMCOG1149:LuxR family
                     transcriptional regulator (Score: 372.8; E-value:
                     6.9e-113)"
                     /gene_kind="regulatory"
                     /note="similar to LuxR transcriptional regulator"
                     /product="ToyA"
                     /protein_id="APT68170.1"
                     /transl_table=11
                     /translation="MYRTDDGPKTSMTGRQNELRRLGAALDDGAHGGTVRALVTGEPGI
                     GRTTLLQACAAAARARGWITVEVRCPPHEHLPPFGLARHLAGRLAALLPPEHPRRTALD
                     AVAHPPADRTGPADPTPVAADVVLGHVRAALTVLTAGAPVLLGLDDAHRADAASLDFLH
                     RALLAPPLARVVLVASARDGENRSGGGPGTLPELLAGARQLPLTGLAPSQSAALLRRVA
                     RRAPAPDVAAECHRACAGNPALLTEVARVLRERPADRAGAPLAAALRPAAVAELIGTRL
                     AGLDPNAGKVALAIAVAETHSAAEPLLVSHLSGLTLAETLAAVDLLVCMHLVADDDTLY
                     LRHRVVREALLGRMTLMARNAAHLAAATHLHGKQAPADRIADHLAASTVTLDEPWAAPV
                     LLRAGQKALAAREPQRARRYLAHAVRAASGADRRTAVLELVDAEIGIDLQAGLREAVRT
                     LEETTEDALRLPLLARISTARYLGTDPGATARTTPAPDYARPDWHPVHALLDGLAAYPL
                     PDTARRTGERLAPEEPGPGCPETGGADCCGADRAAADAVVRALRSHLTCRPADAVHGAR
                     EALRAEGGLCLHPVLRLMALMILAWNGRDADVTSHLRLHHDCGHCHAHPLHRALLLPVT
                     AHLALLRGRLRTAHDRLGASLDALAHYGTRPQHPLLIWLVGMLAEVRLAMGRGPEARAL
                     LHEHGCLDGPAPDRLHREVWLTRARLRADGGDLAGVAADLAELTDAPGGPTGGVHTYVH
                     TVDLLTQVGRHVQARETARTLLRVAEAMGTPRERGIALRVLGALGGAPEGEDLLREAMR
                     LLAAAEADFDLAHAAADLALLLFDDGRYEEATAELSTALELADRCGARPLALRVRQRLA
                     AAEGRSADRSPLGGVLKLTKREKQILIDAVRGSTNERIATTLHITRRTVELHLSSAYRK
                     LDITGRKDFPHLFRTAGLWPLLVDSAAT"
     source          1..10410
                     /country="China: Xixi National Wetland Park, Hangzhou"
                     /db_xref="taxon:42236"
                     /isolation_source="plant rhizosphere"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces diastatochromogenes"
                     /strain="1628"
     misc_feature    1..10410
                     /note="toyocamycin biosynthesis gene cluster"
     subregion       1..10410
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001808"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..10410
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="nucleoside"
                     /region_number="1"
                     /rules="(LipM or LipV or LipU or ToyB or TunD or pur6 or
                     pur10 or nikJ or nikO or truD)"
                     /subregion_numbers="1"
                     /tool="antismash"
     protocluster    48..10410
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[10047:10410]"
                     /cutoff="20000"
                     /detection_rule="(LipM or LipV or LipU or ToyB or TunD or
                     pur6 or pur10 or nikJ or nikO or truD)"
                     /neighbourhood="10000"
                     /product="nucleoside"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10048..10410
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(LipM or LipV or LipU or ToyB or TunD or
                     pur6 or pur10 or nikJ or nikO or truD)"
                     /neighbourhood="10000"
                     /product="nucleoside"
                     /protocluster_number="1"
     cand_cluster    48..10410
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(LipM or LipV or LipU or ToyB or TunD or
                     pur6 or pur10 or nikJ or nikO or truD)"
                     /kind="single"
                     /product="nucleoside"
                     /protoclusters="1"
                     /tool="antismash"
     gene            3033..3749
                     /gene="toyM"
     CDS             3033..3749
                     /codon_start=1
                     /gene="toyM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Asn_synthase"
                     /gene_kind="biosynthetic-additional"
                     /note="similar to ExsB family"
                     /product="ToyM"
                     /protein_id="APT68169.1"
                     /sec_met_domain="Asn_synthase (E-value: 8.1e-14, bitscore:
                     41.3, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDQDDSRTDPFTAVVVLSGGLDSTTLMAHYAALQYRLIAVTVDYG
                     QRHHREIDAARAVAAHFGAEHHVVDLRGYGALLRGSALTDDRVSVPDGHYAEQSMRATV
                     VPNRNAVLANVAVSIGVAHRASVVALGMHAGDHFIYPDCRPEFVAALRELVATANEGFA
                     VPQVEAPFLSWAKTDIATHGVRLGAPLELSWSCYKGGDRHCGTCGTCYERREAFRNAGL
                     ADPTGYLDDVTAYPAP"
     gene            complement(3853..4551)
                     /gene="toyI"
     CDS             complement(3853..4551)
                     /codon_start=1
                     /gene="toyI"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115:HAD-superfamily hydrolase, subfamily IA, variant
                     (Score: 68.1; E-value: 1.4e-20)"
                     /gene_kind="biosynthetic-additional"
                     /note="similar to Haloacid dehalogenase superfamily"
                     /product="ToyI"
                     /protein_id="APT68171.1"
                     /transl_table=11
                     /translation="MVEEMGRTGRTTVVLDRDGTLLDFYEMFHRFVLDLHRQEGVIPPP
                     REEILGFGYWSSITSGALHIGSVRVRDRVDEVAHRYMAHGTLYPGVVPMLSALSGAGVR
                     LALVSSWVGTEATVALLERYAVRPCFGPILTRDDLDAGDGPVSDADCKTALARRALDGL
                     GHAPDHRLYVVGDTPSDIALGRRLGAVVVGVRTGNGGGLPAEGTPEGPDVLLPSAADLG
                     RLILQAEPVG"
     gene            complement(4557..5078)
                     /gene="toyH"
     CDS             complement(4557..5078)
                     /codon_start=1
                     /gene="toyH"
                     /note="similar to phosphoribosyl-pyrophosphate transferase"
                     /product="ToyH"
                     /protein_id="APT68172.1"
                     /transl_table=11
                     /translation="MSGTPMWRRDRLLTISWEAMGRMLAGLADDVRASGFAPTAVAGIA
                     RGGLPPAIALANLLAIEDFRILGIPRNTSNSRYSERGAAVLKYVVPEREFGGARVLLVD
                     DIMGDGGTMALAKRTVEELGAAEVRTVAVVRNLGAAQRADFQAVEVDDWTVFPWEAPPG
                     DNELAVPLRV"
     gene            complement(5075..6130)
                     /gene="toyG"
     CDS             complement(5075..6130)
                     /codon_start=1
                     /gene="toyG"
                     /note="similar to Adenylosuccinate synthetase"
                     /product="ToyG"
                     /protein_id="APT68173.1"
                     /transl_table=11
                     /translation="MPGTVIVGGQWGDEAKGKISSYLAIRDEPAVAVRAGLGPGAGHTV
                     VHAGRTMKLRQLPSAVIRPATRLLLGAGVLIRPEVLLREIAEFSAESRVGVDFRATVID
                     PEHVAAEAADAGLTDRVRSTGSGHGPALAARAMRAARRAEDVPELAPYLTDTVRVVNDA
                     VDAGDRVHVEGTNGFGLSVLYGTYPYTVGKDSTASTAAADAGLGPTAIDEVVLVFRAYP
                     TRVGAGPFRTEMTEAEAERLGIVEYGTVTGRRRRAGTFDLDQAREAVRVNRPTRIALTF
                     LDHVDASCRAVRTGELPEAAAAFVARVEEGLGRPVDLLGTGPGTADVVDRWNGRPAGTA
                     RNVTSGQGGDR"
     gene            complement(6115..7500)
                     /gene="toyF"
     CDS             complement(6115..7500)
                     /codon_start=1
                     /gene="toyF"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1063:argininosuccinate lyase/adenylosuccinate lyase
                     (Score: 133.7; E-value: 1.6e-40)"
                     /gene_kind="biosynthetic-additional"
                     /note="similar to Adenylosuccinate lyase"
                     /product="ToyF"
                     /protein_id="APT68174.1"
                     /transl_table=11
                     /translation="MTNPWEDGRYGSPEMRLLVSDRNRYQLFVRVEGALAAEQGQLGVI
                     PSEAAGAIAARARDHRCDLARIAEIETVSRHELYAVVQQFAETCAPHGRYVHHGVTSSD
                     VIDTALALQLDAAIGLLGERLGVLMSALCRQIAEEGGRVMIGRTHGQHAQPVTLGFKLA
                     VFLDQLTRCLARLAELRERAVRGKVAGAVGSLGGLGPQGMAVQRAVLARLGLPEPQICA
                     QAVARDRIAEFVSWAALTAACLENLATEIRNLQRTEIGELAESFAEGDQIGSSAMPHKR
                     NPVRSERICSLARLLRSLVGPALENVVTWHERDLANSANERFTLPQACVLLDEMLLTTT
                     AVVDDLRVFPERMRENLDRSRHSHLSEAVLLAMVDRGTDRLDAYRALQRASALAARDDR
                     DLVAVLADDASVTAVLPAPDLAALAEAAADTGFCDTLAQETVRRAEEALAQSTEGARTC
                     PAR"
     gene            complement(7497..8642)
                     /gene="toyE"
     CDS             complement(7497..8642)
                     /codon_start=1
                     /gene="toyE"
                     /note="similar to GMP reductase"
                     /product="ToyE"
                     /protein_id="APT68175.1"
                     /transl_table=11
                     /translation="MAVRDIRTGLSLDDVLLVPRRTSVVSRSATDTSTELAGITLRVPV
                     LSANTPWCTGRQMAVAMAVAGGLGIVHRMQTAEDQAAEVAAVKATPVPEGTERATVDAQ
                     GRLRAGAAVGVTDDYLERAARLVEAGADVLVTDVAHGHADYVLASVEKLRARFPDTPLI
                     AGNVATAAGTRDLIEAGAHAVKVGIGPGGICTTRLVAGSGVPQLTAVLDCAEEAAGSGV
                     PVIADGGIKAPGDVVKALAAGGHAVMLGSALAGADESAARLVERDGVPVKVSTGFVTFG
                     MQLTLKRARGEEVSREELDAYTPEGVEATFAHTGPLAATLRPFVGGLRSGMSYSGAHTL
                     AELRAQAEFIRVTPAGLSESRPHALERTQQVALDYAREATG"
     gene            complement(8677..9273)
                     /gene="toyD"
     CDS             complement(8677..9273)
                     /codon_start=1
                     /gene="toyD"
                     /note="similar to GTP cyclohydrolase I"
                     /product="ToyD"
                     /protein_id="APT68176.1"
                     /transl_table=11
                     /translation="MSGAPELDVLDDSGPQVPDPLEDVARKLLIEIGEDPERDGLKETP
                     ARFARWWREFSRYDAGAVDTLFPLQTEGQLVMVSDINVWSLCEHHLLPFSCSLTIAYRP
                     KGDVLGLSKFARIAHRHAHRLQVQERLVRDIADEVTKVTGSVDVAVIATGEHLCMSMRG
                     IRTPAVMTSTAYSGVFEEYGPARQELVATVFPLRK"
     gene            complement(9279..9908)
                     /gene="toyC"
     CDS             complement(9279..9908)
                     /codon_start=1
                     /gene="toyC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_kind="biosynthetic-additional"
                     /note="similar to Radical SAM"
                     /product="ToyC"
                     /protein_id="APT68177.1"
                     /sec_met_domain="Fer4_12 (E-value: 1.8e-11, bitscore: 34.0,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 2.6e-13, bitscore: 40.1,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLGGCNLSCRWCDTPYTWDWTGVGDSGVAYDPKRELRPMPWTDV
                     LRALLAYEVPLIVVSGGEPLSQQRRLLPLLGALTEAGRKVEIETNGTVVPQDDLVALGV
                     RFNVSPKLSHSGDPQARRIVPDALRAFAAVPGTAFKFVCRTTSDLDEVAQLVDRYGTRP
                     VWIMPEGQVPAEVGQHLSALGDDVVARGWNLTTRLHVAVWGDRRGV"
     gene            complement(10048..10410)
                     /gene="toyB"
     CDS             complement(10048..10410)
                     /codon_start=1
                     /gene="toyB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     nucleoside: ToyB"
                     /gene_kind="biosynthetic"
                     /note="similar to 6-pyruvoyltetrahydropterin synthase"
                     /product="ToyB"
                     /protein_id="APT68178.1"
                     /sec_met_domain="ToyB (E-value: 1.5e-64, bitscore: 205.2,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAFRITKEFHFSASHRLAELPEGHPCGRMHGHNYVVELELAAPAD
                     GLDKAGFVRDYGDLKQFSRWLDAEVDHRHLNDVLDRNPSAEHLAQWLYERWLPHYPQLS
                     AVRVSETAKTWAEYRP"
ORIGIN
        1 atgtatcgca cagacgacgg accgaagacg tccatgacgg ggcgtcagaa cgagttgcgc
       61 cggctcggcg cggccctgga cgacggcgcg cacggcggga ccgtccgcgc cctggtcacc
      121 ggcgaaccgg gcatcggccg gaccaccctg ctccaggcgt gcgcggccgc ggcccgcgcc
      181 cggggctgga tcaccgtcga ggtgcggtgc ccgccgcacg aacacctgcc gccgttcggc
      241 ctcgcccggc acctcgcggg ccgactggcc gcgttgctgc cgccggagca cccccgccgc
      301 accgcgctcg acgccgtcgc gcacccgccg gccgaccgta ccggaccggc cgacccgacg
      361 ccggtggcgg cggacgtggt gctgggccac gtcagggcgg ccctcaccgt gctgaccgcc
      421 ggcgcacccg tgctcctcgg cctcgacgac gcccaccggg ccgacgccgc ctcgctggac
      481 ttcctgcacc gggccctgct cgccccgcca ctcgcccgcg tcgtcctggt cgcctccgcc
      541 cgggacggcg agaaccgcag cggcggcggc cccggcacac tgcccgaact cctcgccggc
      601 gcacggcaac tgccgctcac cgggctggcc ccgtcccagt ccgcggcgtt gctccgcagg
      661 gtcgcccgcc gggcaccggc ccccgacgtc gccgcggagt gccaccgcgc ctgtgccggc
      721 aaccccgccc tcctcaccga agtcgcccgg gtgctgcgcg agcggccggc ggatcgcgcc
      781 ggtgcgccgc tggccgccgc actgcggccc gcggccgtcg cggaactgat cggcacccgg
      841 ctggccggac tcgatccgaa cgccggcaag gtcgccctgg ccatcgccgt cgcggagacc
      901 cactccgcag ccgagcccct gctcgtctcc cacctcagcg ggctgaccct ggccgaaacc
      961 ctggccgcgg tcgacctgtt ggtgtgcatg cacctggtcg ccgacgacga cacgctgtac
     1021 ctgcgccacc gcgtcgtgcg cgaggcgctg ctgggccgga tgacgctgat ggcccgcaac
     1081 gccgcccacc tcgcggccgc cacccacctg cacggcaagc aggcgcccgc cgaccggatc
     1141 gccgaccacc tcgcggcgtc gacggtgacc ctggacgagc cgtgggccgc ccccgtgctg
     1201 ctgcgggccg ggcagaaggc cctcgcggcc cgggaaccgc aacgggcccg gcgctatctg
     1261 gcgcacgccg tacgcgcggc gtcgggcgcg gaccgccgga ccgcggtgct ggagctcgtc
     1321 gacgccgaga tcggcatcga cctgcaagcg gggctgcggg aggcggtccg gaccctggag
     1381 gagaccaccg aggacgccct ccggctcccg ctgctggcca ggatcagcac cgcgcgctac
     1441 ctcggaaccg accccggcgc gaccgcccgg acgacgcccg ccccggacta cgcccggccc
     1501 gactggcatc ccgtgcacgc cctgctcgac ggcctggccg cctacccgct gccggacacc
     1561 gcccgacgca ccggggagcg cctcgcgccc gaggagcccg ggccggggtg ccccgagacc
     1621 ggcggggccg actgctgcgg cgcggaccgg gccgccgcag acgccgtcgt gcgcgccctg
     1681 cgcagccacc tgacgtgtcg gccggccgac gcggtgcacg gcgcgcggga ggcgttgcgg
     1741 gcggagggcg gactgtgcct gcacccggtg ctccggctga tggcgctgat gatcctcgcc
     1801 tggaacggcc gcgacgccga cgtgacgtcc cacctgcggc tccaccacga ctgcgggcac
     1861 tgccacgcgc acccgctgca ccgggccctg ctgctgccgg tcaccgccca cctggccctg
     1921 ctgcgcggcc ggctgcgcac cgcgcacgac cggctcggcg catccctcga cgccctggcg
     1981 cactacggga cccggcccca acacccgctg ctgatctggc tcgtggggat gctggccgag
     2041 gtccgcctgg ccatgggacg cggccctgag gcccgcgcgc tgctgcacga acacggctgc
     2101 ctggacggcc cggcaccgga ccgactgcac cgcgaggtgt ggctgacccg ggcgaggctg
     2161 cgggccgacg gcggtgacct ggcgggcgtg gcggcggacc tggccgagct caccgacgcc
     2221 ccggggggac ccaccggcgg cgtgcacacc tacgtccaca ccgtcgatct gctgacgcag
     2281 gtgggccggc acgtgcaggc ccgggagacc gcccggacgc tgctgcgggt cgccgaggcc
     2341 atgggcacac cgcgggaacg cggcatcgcc ctgcgggtgc tcggcgcact gggcggcgcc
     2401 cccgagggcg aggacctgct gcgcgaggcg atgcgcctgc tggccgcggc ggaggccgac
     2461 ttcgacctcg cgcacgccgc cgccgacctg gccctgctgc tgttcgacga cgggcgctac
     2521 gaggaggcga cggcggagct gagcacggcg ctggagctgg cggaccgctg cggcgcccga
     2581 ccgctcgcgc tgcgggtgcg gcagcggctc gccgccgcgg aggggcgctc cgccgaccgg
     2641 tcgccgctgg gcggcgtgct gaagctgacc aagcgggaga agcagatcct catcgacgcc
     2701 gtgcgcggct cgaccaacga acggatcgcg acgaccctgc acatcacccg ccgcacggtc
     2761 gaactccacc tgtccagtgc gtaccgcaag ctcgacatca ccggccgcaa ggacttcccg
     2821 cacctgttcc ggaccgcggg gctgtggccg ctgctggtcg attccgccgc cacctgaccg
     2881 gcgcgcggga cccacccctc ggtaccgggg cgcccgcgct acggcaccgg ccgtgatgtt
     2941 ccgcggcccg gccggccgcc acgatggccc cgggcgccgg gccgccccgc gtcccggtcg
     3001 ttcggtttcc cttcccactc cggaggtttg tgatggatca agacgacagc cgcaccgacc
     3061 cgttcaccgc cgtggtggtg ctgtccggag gtctggactc cacgacgctg atggcgcact
     3121 acgccgccct ccagtaccgg ctgatcgccg tcaccgtcga ctacgggcag cgccaccacc
     3181 gcgagatcga cgcggcccgg gcggtggcgg cccacttcgg cgccgagcac cacgtggtcg
     3241 acctgcgcgg ttacggggcg ctgctgcgcg gctccgcact gaccgacgac cgggtcagcg
     3301 tccccgacgg gcactacgcg gaacagtcca tgcgggccac cgtcgtgccc aaccgcaacg
     3361 cggtgctggc gaacgtcgcg gtgtcgatcg gggtggcgca ccgcgcgtcg gtggtggcgc
     3421 tgggcatgca cgccggcgac cacttcatct acccggactg ccggccggag ttcgtcgccg
     3481 cgctgcgcga actggtcgcg acggccaacg agggcttcgc ggtcccccag gtcgaggcgc
     3541 cgttcctctc ctgggccaag accgacatcg ccacgcacgg cgtccggctg ggcgccccgc
     3601 tggaactcag ctggtcctgc tacaagggcg gggaccggca ctgcgggacg tgcgggacct
     3661 gctacgagcg gcgggaggcg ttccgcaacg ccgggttggc cgacccgacc ggctacctcg
     3721 acgacgtgac ggcgtacccg gcgccctgac gccccgggcg cgcccggccc cgcccacggg
     3781 ccgagcaccg cgacgggccg gactccgtgc ggagtccggc ccgtcggcac gccctcggcg
     3841 ggcggaaggg gctcacccca ccggttccgc ctggaggatc aagcggccca ggtccgcggc
     3901 cgacgggagc aggacgtccg gcccctccgg cgtgccctcg gccggcagcc cgccgccgtt
     3961 gccggtgcgc accccgacca cgacggcccc caaccggcgg cccaacgcga tgtccgacgg
     4021 ggtgtcgccg acgacgtaga gccggtggtc gggcgcgtgg ccgagcccgt ccaacgcccg
     4081 ccgcgccaac gcggtcttgc agtcggcgtc ggagaccggg ccgtcccccg cgtccaggtc
     4141 gtcccgggtc aggatcggcc cgaagcacgg gcggaccgcg tagcgctcca gcagggccac
     4201 ggtggcctcg gtgccgaccc aggagctgac cagcgcgagc cgcaccccgg ccccggacag
     4261 cgccgacagc atcggcacca ccccggggta gagcgtgccg tgcgccatgt accggtgggc
     4321 cacctcgtcg acccggtccc gcacccgcac cgacccgatg tgcagcgccc cgctggtgat
     4381 cgaggaccag tagccgaagc cgaggatctc ctcgcggggc ggcggaatca ccccctcctg
     4441 ccggtggagg tccaggacga agcggtggaa catctcgtag aagtccaaca gggtgccgtc
     4501 ccggtccagc acgaccgtgg tccgccctgt ccgacccatt tcctcaacca tgtcgctcac
     4561 acccgcaacg gtacggcgag ttcgttgtcc ccgggcggtg cttcccacgg gaagaccgtc
     4621 cagtcgtcca cctcgaccgc ctggaagtcg gcccgttggg cggcgcccag gttgcgcacc
     4681 accgcgaccg tgcggacctc cgcggcgccg agctcctcca cggtgcgctt ggccagggcc
     4741 atggtgccgc cgtcgcccat gatgtcgtcg accagcagca cccgcgcgcc gccgaactcc
     4801 cgctccggaa cgacgtactt gagcaccgcc gccccccgct cgctgtaccg ggagttggag
     4861 gtgttgcggg ggatgcccag gatgcggaag tcctcgatcg ccagcaggtt cgccagggcg
     4921 atggccggcg gcagcccgcc gcgggcgatg cccgccaccg cggtcggggc gaagccgctg
     4981 gcccggacgt cgtccgccag tccggccagc atccggccca tcgcctccca actgatcgtc
     5041 aacagccggt cccgccgcca catcggtgtg ccgctcatcg gtcgcctccc tggccgctcg
     5101 tcacgttgcg ggccgtgccg gcgggccggc cgttccaccg gtccaccacg tcggcggtgc
     5161 ccggccccgt cccgagcagg tcgaccggcc ggcccaggcc ctcctcgacc cgggccacga
     5221 acgccgccgc cgcctcgggc aactccccgg tgcgcaccgc gcggcaggac gcgtccacgt
     5281 ggtccaggaa ggtcagcgcg atccgggtgg gccggttgac gcgcaccgcc tcgcgtgcct
     5341 ggtccaggtc gaacgtgccg gcccggcgcc gccgcccggt caccgtgccg tactcgacga
     5401 tgcccagccg ctcggcctcc gcctcggtca tctcggtgcg gaacggcccc gcgcccaccc
     5461 gggtggggta ggcccggaag accagcacca cctcgtcgat cgcggtgggg cccagtccgg
     5521 cgtccgccgc cgcggtgctc gccgtgctgt ccttgccgac ggtgtaggga taggtgccgt
     5581 acagcaccga caggccgaag ccgttggtgc cttcgacgtg cacccggtcc ccggcgtcga
     5641 ccgcgtcgtt caccacccgc acggtgtccg tgaggtaagg ggcgagttcg gggacgtcct
     5701 cggcgcggcg cgccgcacgc atggcgcggg cggccagggc cgggccgtgg ccgctgccgg
     5761 tgctgcgcac ccggtcggtc agccccgcgt cggccgcctc cgcggcgacg tgctcggggt
     5821 cgatgacggt ggcgcggaag tccacgccga cccgggactc ggcggagaac tccgcgatct
     5881 cccgcaacag gacctccggg cggatcaaca cgccggcgcc cagcaggagg cgggtggccg
     5941 ggcggatcac cgcactgggc aactggcgca gtttcatggt gcgtccggcg tgcaccacgg
     6001 tgtggccggc gccgggcccc agtccggcgc gcaccgccac cgcgggctcg tcccggatcg
     6061 ccagatagct ggagatcttc cccttggcct cgtcgcccca ctggccaccg acgatcaccg
     6121 tgccgggcat gtccgagctc cttccgtcga ctgtgcgagc gcttcctcgg cccggcgcac
     6181 ggtctcctgc gccagggtgt cgcagaagcc ggtgtccgcg gccgcctcgg ccagggccgc
     6241 gaggtccggt gcgggcagca ccgcggtcac cgacgcgtcg tccgccagca ccgccaccag
     6301 gtcgcggtcg tcccgggcgg cgagcgcact ggcccgttgc agggcccggt aggcgtcgag
     6361 ccggtccgtc ccgcggtcga ccatggccag cagcaccgcc tcgctgaggt gactgtggcg
     6421 cgaccggtcg aggttctccc gcatccgctc ggggaagacc ctcaggtcgt ccaccacggc
     6481 ggtggtggtg aggagcatct cgtcgagcag cacgcacgcc tgcggcaggg tgaagcgttc
     6541 gttggcggag ttggccaggt cccgttcgtg ccaggtcacc acgttctcca gcgcggggcc
     6601 gaccagcgac cgcagcagcc gggccaggct gcagatgcgt tcgctgcgca ccgggttgcg
     6661 cttgtgcggc atggcggagc tgccgatctg gtccccctcg gcgaaggact cggcgagttc
     6721 gccgatctcc gtccgctgga ggttgcggat ctcggtcgcg aggttctcca ggcaggccgc
     6781 ggtcagcgcg gcccaggaga cgaactccgc gatccggtcc cgcgccaccg cctgggcgca
     6841 gatctgcggc tccggcaggc ccagccgggc cagcaccgcc cgctgcaccg ccatgccctg
     6901 cggtcccagt ccgcccagcg aaccgaccgc accggcgacc ttgccccgca cggcgcgctc
     6961 gcgcagctcc gccagccgcg ccaggcaccg ggtcaactgg tcgaggaaca ccgccagttt
     7021 gaaccccagg gtgacgggct gggcgtgctg gccgtgggtc cgtccgatca tcacccgccc
     7081 gccctcctcg gcgatctgcc ggcacagcgc ggacatcagg acgccgagcc gttcgcccag
     7141 gaggccgatc gccgcgtcca gttggagcgc cagcgcggtg tcgatgacgt cgctgctggt
     7201 caccccgtgg tgcacgtagc ggccgtgggg cgcgcaggtc tcggcgaact gctggactac
     7261 ggcgtagagc tcgtgccggc tcaccgtctc gatctcggcg atccgcgcca ggtcgcaccg
     7321 gtggtcgcgg gcccgggcgg cgatcgcccc ggcggcctcg gacgggatca cccccaactg
     7381 gccctgctcc gcggccaacg ccccctccac ccgtacgaag agctggtagc ggttgcggtc
     7441 gctgaccagc aggcgcatct ccgggctgcc gtagcggccg tcctcccaag ggttcgtcat
     7501 ccggtggcct cccgggcgta gtccagggcc acctgctggg tccgctccag ggcgtgcggg
     7561 cggctctccg acagtccggc cggcgtgacc cggatgaact cggcctgcgc ccgcagctcg
     7621 gccagggtgt gcgccccgga gtagctcatg cccgaccgca gtccgccgac gaacggccgc
     7681 agggtggcgg ccagcggccc ggtgtgggcg aaggtggcct cgacgccctc gggggtgtac
     7741 gcgtccagct cctcgcggct gacctcttcg ccgcgggccc gcttcagggt cagctgcatg
     7801 ccgaacgtca cgaagccggt gctgaccttc acggggacgc cgtcgcgttc caccaggcgg
     7861 gccgcgctct cgtcggcacc ggccagggcg ctgcccagca tcaccgcgtg gccgccggcg
     7921 gccagcgcct tgacgacgtc gcccggcgcc ttgatcccgc cgtccgcgat caccgggacg
     7981 ccggaaccgg ccgcctcctc ggcgcagtcg aggaccgcgg tcagctgcgg caccccgctg
     8041 cccgcgacga gccgggtggt gcagatgccg ccggggccga tgcccacctt cacggcgtgg
     8101 gcgccggcct cgatcaggtc cctcgtgccg gcggcggtgg cgacgttccc ggcgatcagc
     8161 ggcgtgtcag ggaaacgggc ccgcagcttc tcgacggagg ccaggacgta gtccgcgtgg
     8221 ccgtgggcga cgtcggtgac caggacgtcc gcgcccgcct cgacgagccg ggcggcccgc
     8281 tccagatagt cgtcggtcac ccccacggcc gccccggcgc gcagccggcc ctgcgcgtcg
     8341 acggtggccc gctccgtgcc ctcgggcacc ggcgtcgcct tgaccgcggc cacctccgcg
     8401 gcctggtcct cggcggtctg catgcggtgg acgatcccga gcccaccggc caccgccatg
     8461 gcgacggcca tctgccggcc ggtgcaccag ggggtgttgg cggagaggac cggcacccgc
     8521 agggtgatcc cggccagctc ggtgctggtg tcggtcgccg agcggctcac caccgaggtg
     8581 cggcggggca ccagcaggac gtcgtccagg ctgagcccgg tgcgtatgtc gcgaactgcc
     8641 acgtgcgtac tcctgtcgat cgtggacggg gtcgggtcac ttgcgcagcg ggaacaccgt
     8701 cgccacgagt tcctgacggg ccggcccgta ctcctcgaag accccggagt aggccgtgga
     8761 cgtcatgacc gccggggtac ggatgccgcg catgctcatg cacaggtgct cgccggtggc
     8821 gatgaccgcg acgtcgaccg aaccggtcac cttggtgacc tcgtccgcga tgtcgcggac
     8881 caggcgctcc tgcacctgga gccggtgcgc gtggcgatgg gcgatccggg cgaacttcga
     8941 cagtccgagc acgtcgccct tgggccggta ggcgatggtc agggagcagg agaagggcag
     9001 caggtggtgc tcgcacagcg accacacgtt gatgtccgag accatgacca gttgtccctc
     9061 cgtctggagg gggaacagcg tgtcgaccgc ccccgcgtcg tagcgggaga actcccgcca
     9121 ccagcgggcg aagcgggccg gcgtctcctt gagcccgtcg cgctccgggt cctcgccgat
     9181 ctcgatcagc agcttgcgcg cgacgtcctc cagcgggtcc gggacctgcg gcccggagtc
     9241 gtcgaggacg tccagttcgg gggctccgct cactgtgctc acacgcctct cctgtcgccc
     9301 cagacggcaa catgcaatcg agtggtcagg ttccagccgc gtgccacgac gtcgtccccc
     9361 agcgcggaca ggtgttggcc gacctcggcg ggtacctgcc cctcgggcat gatccacacc
     9421 gggcgggtcc cgtaccggtc gacgagctgc gccacctcgt cgaggtcgct cgtggtgcgg
     9481 cagacgaact tgaaggcggt ccccgggacg gccgcgaacg cccgcaacgc gtccggcacg
     9541 atccgccgcg cctgcggatc gcccgagtgc gacagcttgg gcgagacgtt gaaccgcacg
     9601 ccgagcgcca ccaggtcgtc ctgcggcacc acggtgccgt tcgtctcgat ctccaccttg
     9661 cggccggcct ccgtcagcgc gcccagcagc ggcagcaacc ggcgctgctg gctgaggggt
     9721 tcgcccccgg agaccacgat cagcgggacc tcgtaggcga gcagcgcacg cagcacgtcg
     9781 gtccacggca tcggccgcag ttcccgcttg gggtcgtagg cgaccccgga gtcgcccact
     9841 ccggtccagt cccaggtgta cggggtgtcg caccagcggc aggacaggtt gcagccgccc
     9901 aaccgcacga aggcgcaccg ctgcccggcc gaggggccct ctccctgcac ggccctcaag
     9961 tggggccgaa gatctcgttg acgacgagct tcggttccac cgcggcacca cctcctcggt
    10021 gttgcgacgg ggtggcgggc atctgcgtca cggccggtac tccgcccacg tcttggcggt
    10081 ctcggagacc cgcaccgcgc tcaactgcgg gtagtgcggc agccaccgct cgtacagcca
    10141 ctgcgcgagg tgctcggccg aggggttgcg gtccagcacg tcgttgaggt gacggtggtc
    10201 cacctcggcg tccaaccacc ggctgaactg cttgaggtcg ccgtagtcgc ggacgaagcc
    10261 cgccttgtcc aggccgtcgg ccggggccgc gagctccagt tcgacgacgt agttgtgccc
    10321 gtgcatccgg ccgcacgggt gcccttcggg cagctcggcg agccggtgac tggccgagaa
    10381 gtggaactct ttggtgatcc gaaaagccat
//
