BGC0001822 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001822 (Cystobacter sp)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS	1	44,663	vioprolide A/vioprolide B/vioprolide C/vioprolide D	NRPS:Type I	100%

BGC0001822 - Region 1 - NRPS

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Location: 1 - 44,663 nt. (total: 44,663 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 44,664
Label		BGC0001822

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001822	1.00	NRP	vioprolide A, vioprolide B, vioprolide C, vioprolide D	Cystobacter sp.
BGC0001133	0.43	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002071	0.43	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0000426	0.38	NRP	sevadicin	Paenibacillus larvae
BGC0002135	0.36	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002017	0.35	NRP	potensibactin	Nocardiopsis potens DSM 45234
BGC0001844	0.35	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0002437	0.34	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0001135	0.34	NRP	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002518	0.34	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001822	1.00	NRP	vioprolide A, vioprolide B, vioprolide C, vioprolide D	Cystobacter sp.
BGC0001133	0.74	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002071	0.74	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0000426	0.70	NRP	sevadicin	Paenibacillus larvae
BGC0002135	0.69	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002017	0.68	NRP	potensibactin	Nocardiopsis potens DSM 45234
BGC0001844	0.68	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0002437	0.67	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0001135	0.67	NRP	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002518	0.67	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 44663:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ala - D-Leu) + (Pro - Cys) + (Thr - Hpr - Thr) + (NMe-Val)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

vioA

Search NORINE for peptide: strict or relaxed

CAL_domain (47..490): pk

minowa: NH2

Prediction, score:

NH2: 200.6
fatty_acid: 20.4
AHBA: 18.4
shikimic_acid: 8.5
Acetyl-CoA: 0.4

AMP-binding (2796..3195): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHLSLIEK
Nearest Stachelhaus code(s):: Ala DVWHLSLIEK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3860..4254): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGHVVK
Nearest Stachelhaus code(s):: Leu DAWFLGHVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

vioB

Search NORINE for peptide: strict or relaxed

AMP-binding (469..885): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVQCLSEVTK
Nearest Stachelhaus code(s):: Pro DVQCLSEVTK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1564..1963): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code(s):: Cys DLYNLSLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

vioC

Search NORINE for peptide: strict or relaxed

AMP-binding (547..954): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1618..2024): Hpr

nrpys: Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DIQYYAQVVK
Nearest Stachelhaus code(s):: Hpr DIQYYAQVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2690..3098): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

vioD

Search NORINE for peptide: strict or relaxed

AMP-binding (506..907): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWLGGTFK
Nearest Stachelhaus code(s):: Val DAFWLGGTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)