BGC0001842: gacamide A biosynthetic gene cluster from Pseudomonas fluorescens Pf0-1
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Location: 2,495,895 - 2,538,708 nt. (total: 42,814 nt).
This entry is originally from NCBI GenBank CP000094.2.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001842
Short description gacamide A biosynthetic gene cluster from Pseudomonas fluorescens Pf0-1
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
Loci
CP000094.2
2495895 - 2538708
via Knock-out studies, Gene expression correlated with compound production
Compounds
  • gacamide A
Species Pseudomonas fluorescens Pf0-1 [taxonomy]
References
Chemical products information
gacamide A Evidence: NMR, Mass spectrometry
Copy SMILES
C66H115N13O19
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • Pfl01_2210
  • ABA73953.1
 2495895 - 2496689 (-) putative LuxR-family regulatory protein
  • Regulation
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  • Pfl01_2211
  • ABA73954.1
 2497181 - 2503591 (+) putative non-ribosomal peptide synthetase
  • Scaffold biosynthesis
  • Knock-out
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  • Pfl01_2212
  • ABA73955.1
 2503777 - 2520045 (+) putative non-ribosomal peptide synthetase
  • Scaffold biosynthesis
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  • Pfl01_2213
  • ABA73956.1
 2520042 - 2534690 (+) putative non-ribosomal peptide synthetase
  • Scaffold biosynthesis
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  • Pfl01_2214
  • ABA73957.1
 2534757 - 2535905 (+) putative drug-efflux protein
  • Transport
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  • Pfl01_2215
  • ABA73958.1
 2535908 - 2537863 (+) macrolide-specific ABC-type efflux carrier
  • Transport
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  • Pfl01_2216
  • ABA73959.1
 2537929 - 2538708 (-) putative LuxR-family regulatory protein
  • Regulation
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Biosynthesis information

Biosynthetic modules

Name
1
Type
nrps-type1
Genes
Pfl01_2211
Substrates
leucine (evidence: Structure-based inference)
Integrated Monomers
Domains
condensation (Starter), adenylation, epimerase
Name
2
Type
nrps-type1
Genes
Pfl01_2211
Substrates
aspartic acid (evidence: Structure-based inference)
Integrated Monomers
Domains
condensation (Dual), adenylation, epimerase
Name
3
Type
nrps-type1
Genes
Pfl01_2212
Substrates
glutamine (evidence: Structure-based inference)
Integrated Monomers
Domains
condensation (Dual), adenylation, epimerase
Name
4
Type
nrps-type1
Genes
Pfl01_2212
Substrates
isoleucine (evidence: Structure-based inference)
Integrated Monomers
Domains
condensation (Dual), adenylation, epimerase
Name
5
Type
nrps-type1
Genes
Pfl01_2212
Substrates
leucine (evidence: Structure-based inference)
Integrated Monomers
Domains
condensation (Dual), adenylation, epimerase
Name
6
Type
nrps-type1
Genes
Pfl01_2212
Substrates
glutamine (evidence: Structure-based inference)
Integrated Monomers
Domains
condensation (Dual), adenylation, epimerase
Name
7
Type
nrps-type1
Genes
Pfl01_2212
Substrates
serine (evidence: Structure-based inference)
Integrated Monomers
Domains
condensation (Dual), adenylation, epimerase
Name
10
Type
nrps-type1
Genes
Pfl01_2213
Substrates
serine (evidence: Structure-based inference)
Integrated Monomers
Domains
condensation (LCL), adenylation, epimerase
Name
11
Type
nrps-type1
Genes
Pfl01_2213
Substrates
isoleucine (evidence: Structure-based inference)
Integrated Monomers
Domains
condensation (Dual), adenylation
Name
8
Type
nrps-type1
Genes
Pfl01_2213
Substrates
leucine (evidence: Structure-based inference)
Integrated Monomers
Domains
condensation (Dual), adenylation
Name
9
Type
nrps-type1
Genes
Pfl01_2213
Substrates
leucine (evidence: Structure-based inference)
Integrated Monomers
Domains
condensation (LCL), adenylation
Annotation changelog

Entry version: 3

Date
Changes
Submitters
Reviewers
2022-10-07
Update chemical activity to schema version 2.11
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Entry version: 2

Date
Changes
Submitters
Reviewers
2022-09-15
Corrected NRP module activity
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
2022-09-15
Removed gene names of 'No gene ID'
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
2022-09-15
Changed amino acid substrates to lower case
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Entry version: 1

Date
Changes
Submitters
Reviewers
2019-10-16
Submitted
  • (ID: KRKW7VUNYGF36O3Z7FAQ6YXG)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
Detailed domain annotation
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