BGC0001842: gacamide A biosynthetic gene cluster from Pseudomonas fluorescens Pf0-1
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Location: 2,495,895 - 2,538,708 nt. (total: 42,814 nt).
This entry is originally from NCBI GenBank CP000094.2.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
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Gene details
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General
Compounds
Genes
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001842
Short description gacamide A biosynthetic gene cluster from Pseudomonas fluorescens Pf0-1
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP (Other lipopeptide)
Loci NCBI GenBank: CP000094.2
Compounds
  • gacamide A
Species Pseudomonas fluorescens Pf0-1 [taxonomy]
References
Chemical products information
gacamide A
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C66H115N13O19
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • Pfl01_2210
  • ABA73953.1
 2495895 - 2496689 (-) putative LuxR-family regulatory protein
  • Regulation
Sequence-based prediction
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  • Pfl01_2211
  • ABA73954.1
 2497181 - 2503591 (+) putative non-ribosomal peptide synthetase
  • Scaffold biosynthesis
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Sequence-based prediction
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  • Pfl01_2212
  • ABA73955.1
 2503777 - 2520045 (+) putative non-ribosomal peptide synthetase
  • Scaffold biosynthesis
Sequence-based prediction
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  • Pfl01_2213
  • ABA73956.1
 2520042 - 2534690 (+) putative non-ribosomal peptide synthetase
  • Scaffold biosynthesis
Sequence-based prediction
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  • Pfl01_2214
  • ABA73957.1
 2534757 - 2535905 (+) putative drug-efflux protein
  • Transport
Sequence-based prediction
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  • Pfl01_2215
  • ABA73958.1
 2535908 - 2537863 (+) macrolide-specific ABC-type efflux carrier
  • Transport
Sequence-based prediction
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  • Pfl01_2216
  • ABA73959.1
 2537929 - 2538708 (-) putative LuxR-family regulatory protein
  • Regulation
Sequence-based prediction
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NRP-specific information
Subclass Other lipopeptide
Cyclic? yes
Release type
  • Macrolactonization
Lipid moiety 3-OH decanoic acid
NRP-synthases
Gene Modules
Pfl01_2211
Module 1
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Starter
Module 2
Specificity: aspartic acid
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Dual
Pfl01_2212
Module 3
Specificity: glutamine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Dual
Module 4
Specificity: isoleucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Dual
Module 5
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Dual
Module 6
Specificity: glutamine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Dual
Module 7
Specificity: serine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Dual
Pfl01_2213
Module 10
Specificity: serine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 11
Specificity: isoleucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Dual
Module 8
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Dual
Module 9
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Annotation changelog
MIBiG version Submitter Notes
2.0
  • Hidden contributor (ID: KRKW7VUNYGF36O3Z7FAQ6YXG, no GDPR consent given).
  • Submitted
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Corrected NRP module activity
  • Removed gene names of 'No gene ID'
  • Changed amino acid substrates to lower case
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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