BGC0001845: alpiniamide biosynthetic gene cluster from Streptomyces sp. CBMAI 2042
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Location: 103,490 - 169,546 nt. (total: 66,057 nt).
This entry is originally from NCBI GenBank RCOL01000001.1.

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
KnownClusterBlast
NRPS/PKS domains
General information about the BGC

MIBiG accession | BGC0001845
Short description | alpiniamide biosynthetic gene cluster from Streptomyces sp. CBMAI 2042
Status | Quality: questionable The quality level of this entry. Status: active The status of this entry. Completeness: complete Whether the entry covers everything needed for the pathway producing the compound(s)

Remarks "The gene cluster codifies alpiniamide (Nat Prod Commun. 2013 Oct;8(10):1393-6. (PMID 24354182), biosynthesized by a trans-AT-PKS-NRPS hybrid gene cluster. Other results were based on gene cluster prediction, gene knock out in the wild type strain and heterologous expression of the whole gene cluster."
Biosynthetic class(es)

- other (aminocoumarin)
- PKS (Unknown)
- NRPS (Type I)

Loci
RCOL01000001.1
122924 - 152602
via Knock-out studies, Heterologous expression
Compounds

- alpiniamide

Species Streptomyces sp. CBMAI 2042 [taxonomy]
References

- [1] Alkaloids from an endophytic streptomyces sp. YIM66017. Zhou H et al., Nat Prod Commun (2013) PMID:24354182
- [2] Biosynthesis and Ether-Bridge Formation in Nargenicin Macrolides. Pidot SJ et al., Angew Chem Int Ed Engl (2019) PMID:30677204
- [3] The Nargenicin Family of Oxa-Bridged Macrolide Antibiotics. Pidot SJ et al., Chemistry (2020) PMID:31667915

Chemical products information
alpiniamide Evidence: NMR, Mass spectrometry
Copy SMILES
C17H29N1O6
Chemical database entries
PubCHEM
List of genes involved in compound(s) production

Identifiers | Position | Product | Functions | Evidence | Extra
--- | --- | --- | --- | --- | ---
STAN_0115 RLV64595.1 | 122547 - 122963 (-) | glyoxalase/bleomycin resistance protein/dioxygenase |  |  | copy AA seq copy Nt seq
STAN_0116 RLV64596.1 | 122974 - 123393 (-) | transcriptional regulator, AraC family, isolated domain protein |  |  | copy AA seq copy Nt seq
STAN_0117 RLV64597.1 | 123490 - 124734 (-) | Malonyl CoA-acyl carrier protein transacylase |  |  | copy AA seq copy Nt seq
STAN_0118 RLV64598.1 | 124779 - 125843 (-) | 3-oxoacyl-ACP synthase III |  |  | copy AA seq copy Nt seq
STAN_0119 RLV64599.1 | 125840 - 134248 (-) | Erythronolide synthase, modules 1 and 2 |  |  | copy AA seq copy Nt seq
STAN_0120 RLV64600.1 | 134241 - 138713 (-) | 6-deoxyerythronolide-B synthase |  |  | copy AA seq copy Nt seq
STAN_0121 RLV64601.1 | 138738 - 149546 (-) | polyketide synthase of type I |  |  | copy AA seq copy Nt seq
STAN_0122 RLV64602.1 | 149813 - 150991 (-) | Acyl-CoA dehydrogenase |  |  | copy AA seq copy Nt seq
STAN_0123 RLV64603.1 | 151094 - 152239 (-) | ribosomal RNA small subunit methyltransferase |  |  | copy AA seq copy Nt seq
STAN_0124 RLV64604.1 | 152375 - 152554 (-) | hypothetical protein |  |  | copy AA seq copy Nt seq

Biosynthesis information

#### Biosynthetic modules

Name
2
Type
pks-trans-at
Genes
STAN_0121
Substrates
Integrated Monomers
Domains
ketosynthase, methyltransferase, carrier (ACP)
Name
3
Type
pks-trans-at
Genes
STAN_0121
Substrates
Integrated Monomers
Domains
ketosynthase, dehydratase, ketoreductase
Name
4
Type
pks-trans-at
Genes
STAN_0120
Substrates
Integrated Monomers
Domains
ketosynthase, thioesterase, methyltransferase, carrier (ACP), carrier (ACP)
Name
5
Type
pks-modular
Genes
STAN_0119
Substrates
Integrated Monomers
Domains
acyltransferase, ketosynthase, acyltransferase, carrier (ACP)
Name
6
Type
pks-trans-at
Genes
STAN_0119
Substrates
Integrated Monomers
Domains
ketosynthase, dehydratase, ketoreductase, carrier (ACP)
Name
1
Type
nrps-type1
Genes
STAN_0121
Substrates
glycine (evidence: Sequence-based prediction)
Integrated Monomers
Domains
condensation (LCL), adenylation
Annotation changelog

#### Entry version: 1

Date
Changes
Submitters
Reviewers
2022-09-15
Submitted

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2022-09-15
Removed ketoreductase stereochemistry annotation from modules without ketoreductases

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2022-09-15
Removed empty PKS module modifiers

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2022-09-15
Fetch compound properties from PubChem

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2022-09-15
Update comment

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

2022-09-15
Changed amino acid substrates to lower case

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2022-09-15
Released from embargo

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