LOCUS       BGC0001847             11366 bp    DNA     linear   BCT 14-JUL-1999
DEFINITION  Erwinia carotovora CarR (carR), CarA (carA), CarB (carB), CarC
            (carC), CarD (carD), CarE (carE), CarF (carF), CarG (carG), CarH
            (carH), KduI (kduI), KdgT (kdgT), and RexZ (rexZ) genes, complete
            cds.
ACCESSION   BGC0001847
VERSION     BGC0001847
KEYWORDS    .
SOURCE      Pectobacterium carotovorum (Erwinia carotovora)
  ORGANISM  Pectobacterium carotovorum
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Pectobacteriaceae; Pectobacterium.
COMMENT     On Jun 11, 1999 this sequence version replaced U17224.1.
            ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-08 04:57:09
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 11366
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..11366
                     /clone="pUW14203"
                     /db_xref="taxon:554"
                     /mol_type="genomic DNA"
                     /organism="Pectobacterium carotovorum"
                     /strain="ATCC 39048; GS101"
                     /sub_species="carotovora"
     subregion       1..11366
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="Other"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11366
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            691..1425
                     /gene="carR"
     CDS             691..1425
                     /codon_start=1
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /function="transcriptional activator of carbapenem genes
                     carA-H"
                     /gene="carR"
                     /gene_functions="regulatory (smcogs)
                     SMCOG1197:autoinducer-binding transcriptional regulator,
                     (Score: 234.1; E-value: 2.4e-71)"
                     /gene_kind="regulatory"
                     /note="similar to LuxR; requires N-acyl homoserine lactone
                     for activity"
                     /product="CarR"
                     /protein_id="AAC45995.1"
                     /transl_table=11
                     /translation="MDHEIHSFIKRKLKGVGDVWFSYFMMSKNSTSQPYIISNYPEAWM
                     KEYIKKEMFLSDPIIVASLARITPFSWDDNDIVTLRAKNQDVFISSVQHDISSGYTFVL
                     HDHDNNVATLSIANHLEDANFEKCMKNHENDLQMLLVNVHEKVMAYQRAINDQDNPPDN
                     SRNALLSPRETEVLFLVSSGRTYKEVSRILGISEVTVKFHINNSVRKLDVINSRHAITK
                     ALELNLFHSPCEPVVMKHMDAR"
     gene            1606..3117
                     /gene="carA"
     CDS             1606..3117
                     /codon_start=1
                     /function="involved in carbapenem biosynthesis"
                     /gene="carA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     blactam: BLS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177:asparagine synthase (glutamine-hydrolyzing)
                     (Score: 346.1; E-value: 6.2e-105)"
                     /gene_kind="biosynthetic"
                     /note="probable beta-lactam synthetase"
                     /product="CarA"
                     /protein_id="AAD38229.1"
                     /sec_met_domain="BLS (E-value: 1.3e-183, bitscore: 600.2,
                     seeds: 11, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 3.2e-28, bitscore:
                     89.0, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNSFCVVYKGSDTDINNIQRDFDGKGEALSNGYLFIEQNGHYQK
                     CEMERGTAYLIGSLYNRTFLIGLAGVWEGEAYLANDAELLALLFTRLGANALALAEGDF
                     CFFIDEPNGELTVITESRGFSPVHVVQGKKAWMTNSLKLVTAAEGEGALWFEEEALVCQ
                     SLMRADTYTPVKNAQRLKPGAVHVLTHDSEGYSFVESRTLTTPASNQLLALPREPLLAL
                     IDRYLNAPLEDLAPRFDTVGIPLSGGLDSSLVTALASRHFKKLNTYSIGTELSNEFEFS
                     QQVADALGTHHQMKILSETEVINGIIESIYYNEIFDGLSAEIQSGLFNVYRQAQGQVSC
                     MLTGYGSDLLFGGILKPGAQYDNPNQLLAEQVYRTRWTGEFATHGASCYGIDIRHPFWS
                     HSLISLCHALHPDYKIFDNEVKNILREYADSLQLLPKDIVWRKKIGIHEGSSVNQAFAN
                     VLGSTVDNYQTKSRFTYRVYQAFLRGRLSITDVTPSQLKDLIKKD"
     gene            3120..3872
                     /gene="carB"
     CDS             3120..3872
                     /NRPS_PKS="Domain: ECH (6-244). E-value: 2.8e-46. Score:
                     149.6. Matches aSDomain: nrpspksdomains_carB_ECH.1"
                     /codon_start=1
                     /function="involved in carbapenem biosynthesis"
                     /gene="carB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023:enoyl-CoA hydratase (Score: 205.1; E-value:
                     1.7e-62)"
                     /gene_kind="biosynthetic-additional"
                     /product="CarB"
                     /protein_id="AAD38230.1"
                     /transl_table=11
                     /translation="MVFEENSDEVRVITLDHPNKHNPFSRTLETSVKDALARANADDSV
                     RAVVVYGGAERSFSAGGDFNEVKQLSRSEDIEEWIDRVIDLYQAVLNVNKPTIAAVDGY
                     AIGMGFQFALMFDQRLMASTANFVMPELKHGIGCSVGAAILGFTHGFSTMQEIIYQCQS
                     LDAPRCVDYRLVNQVVESSALLDAAITQAHVMASYPASAFINTKRAVNKPFIHLLEQTR
                     DASKAVHKAAFQARDAQGHFKNVLGKKY"
     aSDomain        3138..3851
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_carB_ECH.1"
                     /evalue="2.80E-46"
                     /label="carB_ECH.1"
                     /locus_tag="carB"
                     /protein_end="244"
                     /protein_start="6"
                     /score="149.6"
                     /tool="antismash"
                     /translation="SDEVRVITLDHPNKHNPFSRTLETSVKDALARANADDSVRAVVVY
                     GGAERSFSAGGDFNEVKQLSRSEDIEEWIDRVIDLYQAVLNVNKPTIAAVDGYAIGMGF
                     QFALMFDQRLMASTANFVMPELKHGIGCSVGAAILGFTHGFSTMQEIIYQCQSLDAPRC
                     VDYRLVNQVVESSALLDAAITQAHVMASYPASAFINTKRAVNKPFIHLLEQTRDASKAV
                     HKAAFQARDAQGHFKN"
     gene            3887..4708
                     /gene="carC"
     CDS             3887..4708
                     /codon_start=1
                     /function="involved in carbapenem biosynthesis"
                     /gene="carC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1121:dioxygenase, TauD/TfdA (Score: 40.1; E-value:
                     4e-12)"
                     /gene_kind="biosynthetic-additional"
                     /product="CarC"
                     /protein_id="AAD38231.1"
                     /transl_table=11
                     /translation="MSEIVKFNPVMASGFGAYIDHRDFLEAKTETIKNLLMRQGFVVVK
                     NLDIDSDTFRDIYSAYGTIVEYADEKIGVGFGYRDTLKLEGEKGKIVTGRGQLPFHADG
                     GLLLSQVDQVFLYAAEIKNVKFRGATTVCDHALACQEMPAHLLRVLEEETFEVRVLERG
                     YYVDVSPDGWFKVPVFTDLGWVRKMLIYFPFDEGQPASWEPRIVGFTDHETQAFFQELG
                     AFLKQPRYYYKHFWEDGDLLIMDNRRVIHEREEFNDDDIVRRLYRGQTADI"
     gene            4763..5893
                     /gene="carD"
     CDS             4763..5893
                     /codon_start=1
                     /function="involved in carbapenem biosynthesis"
                     /gene="carD"
                     /product="CarD"
                     /protein_id="AAD38232.1"
                     /transl_table=11
                     /translation="MPNDLYAIYNRYTSRTLFFKYCATATLTHRLTRRLSLFTLKKCLA
                     RPQGRLFSLVNSIYFGGETLEEVQSTATFLARSGIACVLDYAVEGENDETQFDKAMENT
                     LRLIEMSQQTDSLPFVVIKPSSLGSVAVYARQSERLALDEASASAWSRIVTRFSRLFDY
                     ARSHGVHVMVDAEQTAIQPAVDRLVLDMMREFNRDSAVITLTLQFYLKDQLRFLDECYQ
                     RACQDNFLFGVKVVRGAYLEEEKRVNGGVRCFATKQETDRSYNAAVDYIALRLDRIAPF
                     FATHNEESLALIMSSESLRAGRTWVGQLYGLGDHITYSLLQTGFRVCKYLPYGPLDKSL
                     PYLLRRIEENAVASATFKKENKLLQKELLRRLVGGM"
     gene            5893..6171
                     /gene="carE"
     CDS             5893..6171
                     /codon_start=1
                     /function="involved in carbapenem biosynthesis"
                     /gene="carE"
                     /note="probable ferredoxin; 2Fe-2S subgroup"
                     /product="CarE"
                     /protein_id="AAD38233.1"
                     /transl_table=11
                     /translation="MSKFHCHIRDTAIGFPVSEDESVLSSAYEAGVELPYRCASGYCGV
                     CKVRLTSGNVNMDHSGGISRKDIADGYILPCCSVPLSNLEIEPVSSC"
     gene            6165..7031
                     /gene="carF"
     CDS             6165..7031
                     /codon_start=1
                     /function="involved in resistance to carbapenem"
                     /gene="carF"
                     /gene_functions="other (smcogs) SMCOG1226:hypothetical
                     protein (Score: 220.9; E-value: 4.3e-67)"
                     /gene_kind="other"
                     /product="CarF"
                     /protein_id="AAD38234.1"
                     /transl_table=11
                     /translation="MLKKTLIVGLCCTFPLFSAQAVNTVPDEVVVKGGNFYVGSVFGSE
                     DYAAHANTSIASFAITKTEITYRQYHALQEWADTHGYELSGGCNGATFEDCLPPEQDNS
                     LHPVTNVSWWDAVIFANVLSERQQLQPYYLTIDGKTLKRVPEDDNDKLIRENPQALGYR
                     LPTLAEWQVAARGGKKGLAQGTYGQRYAGSEQPDSVAHFPSDSQSFGTVPVTSKRPNAL
                     GLYDMSGNVSEWLNESYAVEGGKTMYYFCGGSYLERTHSLASCDLHTPGFFMPDIGFRL
                     VRTLDGQ"
     gene            7021..7554
                     /gene="carG"
     CDS             7021..7554
                     /codon_start=1
                     /function="involved in resistance to carbapenem"
                     /gene="carG"
                     /product="CarG"
                     /protein_id="AAD38235.1"
                     /transl_table=11
                     /translation="MVNKFVGWLALCAISNTAAALSPVTLKDGINRLDLNQGGGNDYVV
                     VAQFDNNTSHPNLGMTFFVRRPDGGHSIMPVANSNTFTWFDYRLSAAADFLVQDNRLFL
                     SGKHYFLVTARKQGENVFDPTKVVLTIYDFKASRDDPGVPLYEWSERKRVVTQDTYQSV
                     DEAYKEVNEAMLAK"
     gene            7551..8105
                     /gene="carH"
     CDS             7551..8105
                     /codon_start=1
                     /gene="carH"
                     /product="CarH"
                     /protein_id="AAD38236.1"
                     /transl_table=11
                     /translation="MKISVLIASGLLASFGAWAQLSEQDYQQRVQEFFDAEPPLCLGEK
                     QWPVHSPKGDSPWNSGRLHTLVEAGLAYATPEGTGKVYRLSPVGEKNWRQHGDLCYGRM
                     QVSRIEKIDRVNQELTVVYFTYHLTSLESWAHNRSLRFAFSELDNLVGGMETTRYSATI
                     RETLGGAAKLQDYPVPVELDY"
     gene            complement(8197..9033)
                     /gene="kduI"
     CDS             complement(8197..9033)
                     /codon_start=1
                     /gene="kduI"
                     /product="KduI"
                     /protein_id="AAD38237.1"
                     /transl_table=11
                     /translation="MDVRQSVHSEHAKTLDTTELRKKFLIEQIFTPNQYTMTYSHIDRI
                     VVGGIMPVDGEITFDDGIGKQFGVNYFLERRELGLINIGGPAKIVIDGTSYEVGNEEAL
                     YVGKGAKALAFSSLDSAKPAKLYYNSAPAHAVFPTRIITQDDAIKAPLGDVKTCNKRTI
                     CKYLVPEVVETCQLSMGLTRLAEGSNWNSMPTHTHERRMEVYFYFDMAEDTIIFHMMGE
                     PHETRHLVMHNEQAVISPSWSIHTGVGTKNYAFIWGMIGENLTFDDMDHIAMLDLR"
     gene            complement(9046..10002)
                     /gene="kdgT"
     CDS             complement(9046..10002)
                     /codon_start=1
                     /gene="kdgT"
                     /product="KdgT"
                     /protein_id="AAD38238.1"
                     /transl_table=11
                     /translation="MKIKQAIDKIPGGLMLVPLFLGALCNTFTPGAGKYLGSFSNGLIT
                     GTIPILAVWFFCMGASIEFKATGTMLRKSGVLVVTKIATAWVVALIAGTFLPGDGIQNG
                     MLAGISVLALVAAMDMTNGGLYAALMNQYGSKEEAGAFVLMSLESGPLMTMVILGASGI
                     ATFEPQLFVGAVLPFLIGFALGNLDPDLRKLFGNSVQTLIPFFAFALGNTINLSVILQT
                     GFAGIFLGLLVIVVTGIPLILADKFIGGGNGTAGVAASSSAGAAVATPLLIANMAPEFA
                     PVAQQATALVATSVIVTSVLVPIITALWAKRFSPKHA"
     gene            complement(10355..11143)
                     /gene="rexZ"
     CDS             complement(10355..11143)
                     /codon_start=1
                     /gene="rexZ"
                     /gene_functions="regulatory (smcogs) SMCOG1195:IclR family
                     transcriptional regulator (Score: 248.2; E-value: 1.1e-75)"
                     /gene_kind="regulatory"
                     /product="RexZ"
                     /protein_id="AAD38239.1"
                     /transl_table=11
                     /translation="MNIFQQLEHSKAPAAVRLVMIIDYIAKHDEASFTEICTDLSIPKS
                     SVHHLLEVLVVTQLLRQRADGRYVLGLRLFELGGLVIKNIDLRKDTINFMHELVKETEL
                     TCHLGIMDGDNGFFLSKVESPKAIVKTSWEGKKIVFNRMSLGKVLVAWLPQERIESLIA
                     DCTFEYLTPRTITNKEDFLQHLKIVKEQGWAIDDGEDIEEICCMAAPIFNQNGEVIAAI
                     GVNGMQSQYANGKKEKHLASLIKTSKAITAHINSRNIDSR"
ORIGIN
        1 gatcggcgca ctgcgtgcct ttggcgacgc cgcgttacgg cattttcacg ctgatttgga
       61 gcgggctgat gtcgctggtg agcgtcggcg tgctgctggg tgtcggtctg ggctaccttg
      121 ccgcccgcgc gattgcggtg gtgatgagtg agaaaagcgg ttttgtcctg ccggtgacgt
      181 tggaatggga agacatccac ttcgtcctgc tcctgttgct ggtcgccgct gttgtcctga
      241 cgattccggc gatgctgtcc taccgtcaat ctcctgctac ggcgctgcgt ggcgagtaat
      301 agggttgcga ctcagtcgca aaactaagct gtagtggcat cggacgtgta gttctctgcg
      361 tccgatgctg ttttttcctt acctattcat cccttccgat tctcatactc actcaacggc
      421 cttcgtcctt cggtattatg gtttggggcc agtaacgagc agtaatactg ttacttattt
      481 cgctaaataa tatttataat aatagtaact ctatcaatta ttattgaatt attagtatat
      541 aaaatgttga ctgattgtat attaattgat aactttcgac cttgttaaat cctagtgatt
      601 atatttggtg taactaaaaa taattatatt tactctctgg taaagagttg atcttttaat
      661 ctttgagcaa agtcggtaag agagggtaat atggatcatg aaatccattc ctttatcaaa
      721 aggaagttga aaggagtcgg tgatgtatgg ttttcttatt ttatgatgag taaaaactct
      781 accagccaac cttatattat ttcgaattat ccagaagcat ggatgaagga gtatataaaa
      841 aaagagatgt ttctgagtga tcctatcatt gttgcctcat tagctcggat cacgccgttt
      901 tcttgggatg ataatgatat tgtgacgcta agagccaaga atcaggatgt ctttatttct
      961 tccgtgcagc acgatataag ttcaggttat acctttgttt tgcacgacca tgataataat
     1021 gtggcgacac tgagtatagc gaatcacttg gaagatgcga atttcgaaaa atgtatgaag
     1081 aatcatgaaa atgatttgca gatgttactt gtgaatgtac atgaaaaagt gatggcctat
     1141 cagcgagcta tcaacgatca agataacccc cccgataatt caagaaacgc cttactctct
     1201 ccgcgtgaaa ccgaagttct tttcctggtt agtagtggac gaacttacaa agaggtttcc
     1261 cgtatattag gtattagtga ggtcaccgtt aagttccaca ttaacaactc agtccgtaaa
     1321 ttggatgtta tcaattcccg ccatgctata actaaagcac ttgagttaaa tcttttccat
     1381 tccccctgtg aacctgtagt gatgaagcat atggacgccc gttagcgtgt attaatgctt
     1441 gataataggg aggtacctat ccaaaagaat agtaactccc tatctaaaag aaatacatta
     1501 gctgaacttt tacacggtta atatttaccc tcgccttttc tatgatatgt gacgaattca
     1561 aacgttgttt tctctcatgt tgttattaag gtaagggtta ttactgtgag caatagtttt
     1621 tgcgttgttt ataaaggttc tgataccgat ataaataata tccaacgcga cttcgacgga
     1681 aagggcgaag cattatctaa tggctatctt tttatcgaac agaatggcca ttatcagaag
     1741 tgtgagatgg aaagaggaac ggcctacctg ataggctcgc tgtacaatcg gacgtttctg
     1801 atcggattgg ccggtgtgtg ggaaggcgag gcttatctgg caaatgatgc cgagctgtta
     1861 gcgttgctgt tcacgcgttt gggagcgaat gcactggcgc tggctgaagg tgacttctgc
     1921 tttttcattg atgaaccaaa cggcgaattg acggtgatta ccgagtcgcg tggtttctcg
     1981 ccggttcatg tcgtacaggg caaaaaagcc tggatgacca atagccttaa actggttact
     2041 gcggcagaag gtgaaggcgc gctgtggttt gaagaagagg cgttggtgtg ccagtcgctg
     2101 atgcgagcgg atacctatac gccggtgaaa aatgcgcagc gtcttaagcc gggagcggtg
     2161 catgttctta cgcacgatag cgaaggttat tctttcgttg aaagccgcac gctgaccaca
     2221 ccagccagca accaattgtt agcgctcccg cgtgaaccgc tgctggcatt gattgatcgc
     2281 taccttaatg ctccgcttga ggatttagcg ccgcgctttg ataccgtagg aattcccttg
     2341 tcaggcggtc tggattccag cctggtaacg gcgctcgcca gtcgtcattt caaaaaattg
     2401 aatacgtatt cgattggtac ggaactcagc aatgagtttg agttttctca acaggttgct
     2461 gatgcactcg gtacacatca tcagatgaaa attctgtccg aaactgaagt gatcaacggc
     2521 atcatcgaat ccatctatta caacgaaata tttgacggct tatccgctga aatccaatcc
     2581 gggttgttca atgtctatcg tcaggctcag gggcaggtgt cttgcatgct caccggatat
     2641 ggttccgacc tgctctttgg cggcatactg aaaccaggag cgcagtatga caatccgaat
     2701 cagctgcttg ccgagcaagt gtaccggacg cgttggacag gggagtttgc tacccacggt
     2761 gcttcctgtt acggcattga tattcgccac cccttctgga gccattccct aatctctcta
     2821 tgtcatgcgc tacatcctga ttacaaaatt ttcgacaacg aagtcaaaaa catcctgcgt
     2881 gaatacgccg attcgctgca attgctgccg aaagacattg tctggcgcaa gaaaatcggc
     2941 attcacgaag gttcctccgt caatcaggcc tttgcgaatg ttctcgggtc aacggttgat
     3001 aactaccaga ccaaaagtcg ctttacctac cgtgtttatc aagccttcct tcgtggccgt
     3061 ctctccatta cagatgtgac gccctctcag cttaaagatc tgattaaaaa ggattaatta
     3121 tggtttttga agagaattcc gatgaggtcc gagtgatcac gctcgatcat ccgaacaagc
     3181 ataacccctt tagtcgaacg ctggaaacca gcgtgaagga cgcgctggcg cgagccaacg
     3241 ctgacgacag cgtacgagct gtcgtggtgt atggcggggc cgagcgttcc ttttccgctg
     3301 gcggagattt caacgaagtt aagcagctat cgcgcagcga ggacatcgaa gagtggattg
     3361 accgcgtgat tgatttgtat caggccgtcc tgaatgtgaa taaaccgacg atcgcagcgg
     3421 tggatggcta tgcgattggt atgggtttcc agttcgccct gatgtttgac cagagactga
     3481 tggcgtcgac agcaaatttt gtgatgccgg aacttaagca tggtatcggg tgctcggttg
     3541 gggcggccat tctgggattc acccacggat ttagcacaat gcaggaaatc atctaccagt
     3601 gccagtcgct cgatgctcca cgctgtgtgg actaccggct ggttaatcag gtggtggaga
     3661 gcagtgcgct gctggatgcc gctatcacac aggcgcacgt gatggctagc tacccggctt
     3721 ctgcattcat caatacgaaa cgagcggtta acaaaccgtt catccatcta ctggaacaaa
     3781 cccgtgacgc ttccaaagct gtccataagg cagcgttcca ggctcgtgac gctcagggac
     3841 atttcaaaaa tgtgcttggc aaaaaatact gaggataagg aagaaaatga gcgaaatagt
     3901 gaagtttaat ccggtcatgg catccggttt tggcgcgtat atcgatcatc gggactttct
     3961 cgaagccaag acagaaacga ttaaaaactt actgatgcgt cagggatttg tcgtggtcaa
     4021 aaatctcgat attgatagtg acacattccg cgatatctac tcggcttacg gtacgatcgt
     4081 ggagtatgcg gatgaaaaga tcggcgtagg tttcggctat cgcgatacgt tgaagctgga
     4141 aggggaaaaa ggaaaaatcg ttaccggacg tggtcaactt cctttccacg ctgatggcgg
     4201 cctgctgctg tcacaggtag atcaggtttt cctctacgcg gccgagatta aaaacgtcaa
     4261 attccgtggt gcaacaacgg tatgcgacca tgctctggct tgtcaggaaa tgccggctca
     4321 ccttctgcgc gtactggaag aggaaacgtt cgaagttcgt gtgctagagc ggggctatta
     4381 cgtggatgtt tcaccagacg gttggttcaa ggtgccggtc ttcaccgatc tgggatgggt
     4441 cagaaagatg ctgatttatt tcccatttga cgaaggacaa ccggctagct gggagccgcg
     4501 gattgtcggt ttcaccgatc atgaaaccca ggcattcttc caggaactcg gggcgttttt
     4561 aaaacagcca cgctactact acaaacactt ctgggaagat ggtgacctgc tgattatgga
     4621 caaccgtcgt gtcattcatg agcgtgaaga gtttaacgat gacgacatcg tacgtcgtct
     4681 gtatcgcgga caaaccgccg atatctaact ctcatgattc cggcgcgaaa acgtgccgga
     4741 ttgatatgga tctggaacca tcatgcctaa cgatctttac gctatctaca atcgttacac
     4801 gtcaaggacc ctgtttttta aatactgtgc gacagcgacg ctaacgcaca gactgacacg
     4861 tcggctgtcg ctttttacgt tgaaaaagtg ccttgcccgt ccacagggac gccttttctc
     4921 tctggttaat agcatttatt ttggcggaga aacgctggaa gaggttcaga gtacagcaac
     4981 ttttctggca cgctctggca ttgcttgcgt actggactac gccgtggaag gggaaaatga
     5041 cgagacgcag ttcgataagg caatggaaaa tacgctgcgc cttatcgaga tgtcgcagca
     5101 aacggatagc ctgccttttg tcgtgattaa accgtcgtct ctgggtagtg ttgctgtgta
     5161 tgctcggcaa agtgaaaggc tcgcgctgga tgaagcatcc gccagcgcat ggtcacgcat
     5221 cgttacgcgt ttctcacgtc tgttcgatta tgcccgctct catggcgtgc atgtgatggt
     5281 tgatgcagag cagaccgcga ttcagcccgc agtggatcgt ttggttctag acatgatgcg
     5341 tgaatttaac cgcgatagcg cggtgatcac gctgacgctg caattttatc taaaggatca
     5401 attgcgtttt ctcgacgaat gctatcagcg agcctgtcag gataatttcc tgttcggcgt
     5461 gaaagtagtg cgcggtgctt atctggaaga agagaaacga gtgaacggtg gcgtacgctg
     5521 ttttgcaacc aaacaggaaa ccgatcgtag ttataacgcg gcggtggatt acattgcact
     5581 gcgtctggat cgaatcgcac cgtttttcgc cacgcacaac gaggaaagcc tcgctttgat
     5641 tatgtcgagt gagtcgctac gcgcggggcg cacctgggta ggccagcttt acgggttggg
     5701 cgatcatatt acttattcgc tgctgcaaac cggtttccgc gtgtgcaaat acctgcctta
     5761 cggtccgctc gataagtcgt tgccctattt actccgcagg attgaagaga acgcggttgc
     5821 ctcggcaacc ttcaaaaagg aaaataaact cttgcagaag gagttgctgc gccgtctcgt
     5881 aggagggatg taatgtcaaa atttcattgt catattcgcg atacggctat cggtttcccc
     5941 gtgtcggagg atgaaagcgt actcagttca gcctatgaag ctggtgtcga actgccttat
     6001 cgctgtgctt ctggttactg tggtgtatgc aaggtgcgcc tgacgtccgg caacgtcaac
     6061 atggatcatt ctggtgggat ctcacgtaaa gatatcgctg atggttacat tctaccttgc
     6121 tgttctgtgc cgctctctaa cctcgaaatt gaacctgtat cgtcatgctg aagaaaacgc
     6181 taattgttgg actgtgctgt actttccccc tcttctccgc tcaagcggtg aatactgttc
     6241 ctgacgaggt ggttgtcaaa ggtggcaatt tctatgttgg atcggtcttc ggctcggaag
     6301 actatgctgc tcatgccaac acgtctatcg cgtcttttgc catcacgaaa acggaaatta
     6361 cctatcggca gtatcatgcg ttgcaagaat gggcggacac acacggttat gagcttagtg
     6421 gcggctgcaa tggcgcgact ttcgaagatt gcttaccgcc tgaacaggat aacagtcttc
     6481 atcctgtcac gaatgtatct tggtgggatg cagtgatttt tgccaatgtg ctcagtgaac
     6541 ggcagcagtt gcagccctat tacctcacga ttgacggtaa gacgctgaaa cgcgtgccgg
     6601 aggacgataa cgataaactc atacgcgaga acccgcaggc tttggggtat cgcttgccga
     6661 cgctggcgga atggcaagtg gcagccagag gcggaaagaa ggggctggcg caggggacgt
     6721 acggacagcg ctatgccggt agcgaacagc cggacagtgt ggcgcatttt ccttctgact
     6781 ctcaatcatt cggcacggtg cctgtgacct cgaagcgccc caatgctctg gggctttacg
     6841 atatgagtgg caatgtatcc gagtggctga atgagtctta tgcggtggaa ggcggcaaaa
     6901 ccatgtacta cttttgcggc ggaagttatc tggaacgcac gcacagtctt gctagctgcg
     6961 atctgcatac gcccggtttt ttcatgccgg atattggttt tcgactagta aggacacttg
     7021 atggtcaata agttcgtagg gtggctggcg ctctgtgcca ttagcaacac ggctgctgca
     7081 ctctctcctg tgacattgaa agacggcatc aatcggctgg atctcaatca gggcggcggg
     7141 aacgattatg tcgttgttgc ccaatttgac aacaatacct cgcatcctaa tctcgggatg
     7201 accttttttg tccgccgtcc agacggtggg catagcatta tgccggtagc caacagcaat
     7261 acgtttacct ggttcgacta tcgcctgtcc gctgcggcgg attttctggt gcaggacaat
     7321 cggctattcc tgtctggaaa gcattacttt ctggtgacgg cacggaagca gggtgaaaac
     7381 gtctttgatc ccacaaaagt cgttttaaca atttacgact ttaaagcctc acgggacgat
     7441 cccggtgtac cactctatga atggtcggag cgcaagcgtg tggtaacaca agatacctac
     7501 caatcggtcg atgaggccta caaggaagtg aatgaggcga tgctggcaaa atgaaaatat
     7561 cggtattaat cgcatccggg ctattggcat ctttcggggc gtgggcgcaa ctcagtgagc
     7621 aggattacca gcaacgcgta caggagtttt ttgatgcaga accgccgctg tgtctgggcg
     7681 agaagcagtg gccggttcat agtcccaaag gtgattctcc ctggaacagt ggacggttgc
     7741 atacgcttgt ggaagcgggg ttggcatacg cgacgcctga aggaacagga aaggtatatc
     7801 ggttatcgcc tgtaggtgag aagaattggc gtcaacatgg cgacctttgc tatggccgta
     7861 tgcaagtgag ccgtattgag aaaatcgatc gtgtgaacca ggaactgacg gtagtgtatt
     7921 tcacttatca tctgacatcg ttggaaagtt gggctcataa ccgttcgtta cgctttgctt
     7981 ttagcgagtt ggataatctt gtcggtggaa tggaaaccac gcgttattcc gctacgattc
     8041 gtgagacgtt gggcggggcg gccaaattgc aggattaccc cgtgccggta gagctggatt
     8101 actaatctga aatcttattg acgtgacata aaaaaaacag ggtgaacgtg gttcaccctg
     8161 ctgcatttta ctgtcttggc gtgacgccag aggggattag cgtaaatcca gcatcgcgat
     8221 atggtccata tcgtcaaacg ttaggttttc gccgatcatg ccccagataa aggcatagtt
     8281 tttggtgccg acaccggtgt gaatcgacca actcggggag atcacggcct gctcgttatg
     8341 catcaccaga tggcgcgttt catgcggttc acccatcata tggaaaataa tggtgtcttc
     8401 cgccatatca aaatagaagt acacttccat tctgcgctca tgggtatgcg tcggcattga
     8461 attccagttg ctgccttctg ccagacgcgt taaccccatg ctcagctggc aagtttccac
     8521 cacttccggc accagatatt tgcaaatcgt ccgcttgttg caggttttga cgtcgcccag
     8581 tggtgctttg atcgcgtcat cctgtgtaat gatgcgtgtc gggaaaacgg catgtgctgg
     8641 tgcgctgttg taatacagtt tcgcaggctt ggcgctgtcc aggctgctga acgctaaggc
     8701 tttcgccccc ttgccgacat agagcgcttc ctcattgcca acttcatagc ttgtgccgtc
     8761 gatgacgatt tttgccgggc cgccaatatt gatcagtccc agctcgcgac gctcaagaaa
     8821 atagttcacg ccaaattgtt tgccgatgcc gtcatcaaac gttatctcgc catccaccgg
     8881 catgatcccg cctaccacga tacggtcgat gtggctgtag gtcatggtgt attggtttgg
     8941 cgtaaatatc tgctcaataa gaaatttctt tctcagctca gtggtatcaa gcgttttcgc
     9001 atgttcactg tgtacacttt gtcgtacgtc cattttgcta ccttcttatg cgtgtttagg
     9061 tgaaaagcgt tttgcccata atgccgtgat gatgggcacc aatactgatg tcacgatcac
     9121 actggtggcg actaacgctg ttgcctgttg agcgaccgga gcgaattccg gagccatatt
     9181 cgcgatcaac agaggagtgg cgacggcggc acccgcgctg ctggatgcgg ccacgcctgc
     9241 ggttccatta ccaccgccga taaatttatc cgccagaatc aatgggatac ctgtaacaac
     9301 aatcacgagc aggccgagga agataccggc gaagcccgtc tggaggatca ctgataaatt
     9361 aatggtgtta cctaacgcaa aggcaaagaa aggaatcagc gtttgtactg aattaccgaa
     9421 cagctttctc aagtccggat ccaggttgcc caatgcaaaa ccaatcagga aaggcagaac
     9481 ggcaccgaca aataactgag gttcaaaggt agcgataccg ctggcaccca gaatgaccat
     9541 ggtcatcaac gggccggatt ccagagacat cagtacgaag gcacccgctt cttctttcga
     9601 accgtattgg ttcatcagtg cagcgtacaa accgccgtta gtcatgtcca ttgccgcgac
     9661 cagcgccagc acggagatcc cagccagcat gccattttgg atgccgtcac ccggcaggaa
     9721 cgtgcccgcg atcagcgcca caacccaggc ggtggcgatt ttggtgacca ccagaacgcc
     9781 tgattttctc agcatcgttc ccgttgcttt aaattcaatg gacgcgccca tgcagaaaaa
     9841 ccataccgcc agaataggga ttgtgccagt aatcaggccg ttactgaaag aacctaagta
     9901 tttccccgcg cctggcgtaa aggtattaca gagtgcgccc aaaaagagcg gcaccagcat
     9961 taatccccca gggattttat caatagcttg tttgattttc ataaaagacc tcattaaaag
    10021 ataaaagcat gttcagcccg ctttcagtga aagcagatgg agtattggta taaagtcata
    10081 aatagggtgg aataaaaccc agggggttat tacccgattt cattaattag tgaagatggt
    10141 tgtccccatt ataaaattga taattaatat ttttcgtcat cattaataat gacttatgtc
    10201 atgttttttt agttatagat ggccttcccg taatgcgtat tagcatttac aggtaattat
    10261 gtcatacgtg tccgtcacgc tttttgcgtg tcattatttc tgggttgttt taataagcca
    10321 gaatttttaa ttatgtaaag cgacggtaaa tgttttatct actatcaata ttccggctgt
    10381 taatatgagc ggtaattgct ttgctggttt taatcaggct tgccagatgc ttttcttttt
    10441 ttccattcgc atattgcgac tgcataccgt taacgccaat ggcggcgatg acttcaccat
    10501 tttggttaaa gattggcgcg gccatacagc agatctcttc tatatcttcg ccgtcgtcta
    10561 tggcccagcc ctgttccttg acgattttca ggtgctgcag gaaatcttct ttattggtaa
    10621 tcgtccgggg cgtcaaatac tcaaaggtgc aatcggcaat cagtgattca attctttcct
    10681 gtggtagcca ggcgacgagt actttcccca gagacatgcg gttaaataca attttcttac
    10741 cttcccagga tgtttttacg atggccttgg gggattcgac tttactcagg aagaaaccgt
    10801 tatcgccatc cataatacca agatggcagg tcagttctgt ctctttgact aattcatgca
    10861 taaagttaat cgtgtcttta cgcagatcaa tattctttat taccaaacca ccaagctcaa
    10921 acagtcttaa tcccaaaaca taacgaccgt cagcacgttg acgtaacaat tgcgtcacca
    10981 ccagcacttc cagtagatga tgtacgctgc ttttgggtat agacaaatca gtgcaaatct
    11041 cagtgaagct ggcttcgtca tgtttggcga tgtagtcaat aatcatcact aaacggacgg
    11101 ctgctggcgc tttactgtgt tcgagctgtt ggaatatatt cattttgttc tatatataaa
    11161 actatggttt cctatataga acaataataa cgcaacggta atttatttac ctagatgcag
    11221 atcacaaaat aatcgcgaag tggttgaata tttcaatata aatgaaacgt agttttatta
    11281 gggaaagaaa atggcggtat tttggcagga aagatgcctg accccgtaaa taaggtcagg
    11341 catgatttaa atgacagaac ggtggt
//
