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Overview
1.1

### Identified secondary metabolite regions using strictness 'relaxed'

BGC0001856 (Streptomyces caniferus)

Region | Type | From | To | Similarity Confidence | Most similar known cluster
--- | --- | --- | --- | --- | ---
Region 1 | T1PKS,NRPS-like,PKS-like | 1 | 186,923 | High | caniferolide A/caniferolide B/caniferolide C/caniferolide D | PKS:Type I

BGC0001856 - Region 1 - NRPS-like,PKS-like,T1PKS
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
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Location: 1 - 186,923 nt. (total: 186,923 nt) Show pHMM detection rules used
Region on contig edge.
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
PKS-like: (t2pks2 or ksIII)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
MIBiG
Tailoring
MIBiG comparison
KnownClusterBlast
NRPS/PKS modules
NRPS/PKS domains
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
External annotation
This annotation was made externally by 'MIBiG' and not by antiSMASH

Name |  | MIBiG
Version |  | 4.0alpha

Type |  | Subregion
Location |  | 1 - 186,924
Label |  | BGC0001856

Tailoring functions
Tailoring enzymes detected in this region
Oxidoreductases: oxidoreductase oxygenase
scaCP1: cytochrome P450

smcogs
  SMCOG1007: cytochrome P450

scaCP2: cytochrome P450

smcogs
  SMCOG1007: cytochrome P450

scaR3: oxidoreductase

smcogs
  SMCOG1079: oxidoreductase

Transferases: PPTase acetyltransferase acylase aminotransferase glycosyltransferase sulfotransferase transferase
scaD: GCN5-related N-acetyltransferase

smcogs
  SMCOG1111: GCN5-related N-acetyltransferase

scaDH3: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs
  SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

scaGS: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs
  SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

scaGT1: glycosyltransferase, MGT family

smcogs
  SMCOG1062: glycosyltransferase, MGT family

scaGT2: glycosyltransferase, MGT family

smcogs
  SMCOG1062: glycosyltransferase, MGT family

scaK: acetyl-CoA carboxylase, carboxyl transferase,

smcogs
  SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

scaP1: malonyl CoA-acyl carrier protein transacylase

smcogs
  SMCOG1021: malonyl CoA-acyl carrier protein transacylase

scaPT: 4'-phosphopantetheinyl transferase

smcogs
  SMCOG1060: 4'-phosphopantetheinyl transferase

scaST: Sulfotransferase domain

extras
  Sulfotransfer_3: Sulfotransferase domain

Hydrolases: dehalogenase esterase
scaN: haloalkane dehalogenase

smcogs
  SMCOG1262: haloalkane dehalogenase

scaT: thioesterase

smcogs
  SMCOG1004: thioesterase

Lyases: dehydratase
scaDH2: NAD-dependent epimerase/dehydratase

smcogs
  SMCOG1010: NAD-dependent epimerase/dehydratase

scaR1: NAD-dependent epimerase/dehydratase

smcogs
  SMCOG1010: NAD-dependent epimerase/dehydratase

scaR2: NAD-dependent epimerase/dehydratase

smcogs
  SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase
scaG: AMP-dependent synthetase and ligase

smcogs
  SMCOG1002: AMP-dependent synthetase and ligase

scaL: AMP-dependent synthetase and ligase

smcogs
  SMCOG1002: AMP-dependent synthetase and ligase

scaP2: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

scaP3: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

scaP4: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

scaP5: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

scaP6: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

scaP7: Beta-ketoacyl synthase

smcogs
  SMCOG1093: Beta-ketoacyl synthase

scaS: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs
  SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters
Shows areas that are similar to the current region to a reference database.
Mouseover a score cell in the table to get a breakdown of how the score was calculated.The MIBiG database.
Click on an accession to open that entry in the MIBiG database.
Analysis type:

Reference | T1PKS | NRPS-like | PKS-like | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | --- | --- | ---
BGC0001856.4 |  |  |  | 1.53 | PKS | caniferolide A, caniferolide B, caniferolide C, caniferolide D | Streptomyces caniferus
BGC0001276.3 |  |  |  | 1.34 | PKS | 6-methylsalicyclic acid | Aspergillus terreus
BGC0001249.5 |  |  |  | 1.13 | NRPS | dimethylcoprogen | Alternaria alternata
BGC0001273.3 |  |  |  | 1.10 | PKS | asperlactone | Aspergillus ochraceus
BGC0001160.5 |  |  |  | 1.06 | PKS | 1-nonadecene, (14Z)-1,14-nonadecadiene | Synechococcus sp. PCC 7002
BGC0001164.3 |  |  |  | 1.06 | PKS | 1-heptadecene | Cyanothece sp. PCC 7822
BGC0002191.2 |  |  |  | 0.98 | PKS | prolipyrone B, gibepyrone D | Fusarium graminearum PH-1
BGC0001268.4 |  |  |  | 0.93 | NRPS, PKS | fusarin C | Fusarium fujikuroi
BGC0001254.3 |  |  |  | 0.91 | PKS | ACT-Toxin II | Alternaria alternata
BGC0002192.3 |  |  |  | 0.91 | PKS | FR901512 | fungal sp. No.14919

Reference | Aggregated | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0001856.4 |  | 1.00 | PKS | caniferolide A, caniferolide B, caniferolide C, caniferolide D | Streptomyces caniferus
BGC0001273.3 |  | 0.75 | PKS | asperlactone | Aspergillus ochraceus
BGC0001276.3 |  | 0.75 | PKS | 6-methylsalicyclic acid | Aspergillus terreus
BGC0001244.3 |  | 0.75 | PKS | (-)-Mellein | Parastagonospora nodorum
BGC0000041.3 |  | 0.72 | PKS | oronofacic acid | Pseudomonas syringae
BGC0001164.3 |  | 0.70 | PKS | 1-heptadecene | Cyanothece sp. PCC 7822
BGC0001249.5 |  | 0.70 | NRPS | dimethylcoprogen | Alternaria alternata
BGC0001160.5 |  | 0.70 | PKS | 1-nonadecene, (14Z)-1,14-nonadecadiene | Synechococcus sp. PCC 7002
BGC0002191.2 |  | 0.66 | PKS | prolipyrone B, gibepyrone D | Fusarium graminearum PH-1
BGC0001268.4 |  | 0.65 | NRPS, PKS | fusarin C | Fusarium fujikuroi

Similar known clusters from MIBiG 4.0
Shows clusters from the MIBiG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Detailed help and explanations are available here.
Click on reference genes to show details of similarities to genes within the current region.
Double click on a reference drawing to reverse the display of the genes.
Click on an accession to open that entry in the MIBiG database.
Location:
Identifier
Identity
Coverage
Module view
Shows module structures for each candidate cluster in NRPS and PKS regions.
Genes are shown in predicted order, and are only present when containing at least one complete module.
A domain glossary is available here, and an explanation of the visualisation is available here.
Legend
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A domain glossary is available here, and an explanation of the visualisation is available here.
Selected features only
Show module domains
NRPS/PKS products
NRPS/PKS substrates
Predicted core structure(s)
Shows estimated product structure and polymer for each candidate cluster in the region. To show the product, click on the expander or the candidate cluster feature drawn in the overview.
For candidate cluster 1, location 0 - 161352:
Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:
  (Me-mal - Me-ohmal - Me-ccmal) + (Me-ohmal - Me-ohmal - ccmal) + (ccmal - redmal) + (ohmal - redmal - ohmal) + (ohmal - ohmal - mal - Me-ohmal - redmal) + (ohmal - redmal - ohmal) + (Me-ohmal - ccmal) + (AHBA - pk) + (Asn)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 160286:
Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:
  (Me-mal - Me-ohmal - Me-ccmal) + (Me-ohmal - Me-ohmal - ccmal) + (ccmal - redmal) + (ohmal - redmal - ohmal) + (ohmal - ohmal - mal - Me-ohmal - redmal) + (ohmal - redmal - ohmal) + (Me-ohmal - ccmal) + (AHBA - pk) + (Asn)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 120320 - 161352:
Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:
  (Asn) + (AHBA - pk) + (Me-ohmal - ccmal)

Direct lookup in NORINE database: strict or relaxed
Link to NORINE database query form
NRPS/PKS substrate predictions
Shows the predicted substrates for each adenylation domain and acyltransferase within genes. Each gene prediction can be expanded to view detailed predictions of each domain. Each prediction can be expanded to view the predictions by tool (and, for some tools, further expanded for extra details).
scaP1: mmal - mmal - mmal
PKS_AT (559..856): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 221.6
  Ethylmalonyl-CoA: 219.6
  Methoxymalonyl-CoA: 173.1
  2-Methylbutyryl-CoA: 166.7
  trans-1,2-CPDA: 136.4
  CHC-CoA: 112.9
  Propionyl-CoA: 112.0
  Malonyl-CoA: 108.9
  Isobutyryl-CoA: 63.0
  Benzoyl-CoA: 55.4
  Acetyl-CoA: 55.2
  fatty_acid: 25.9
  inactive: 23.3
  3-Methylbutyryl-CoA: 19.6

PKS_AT (1570..1867): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 225.9
  Ethylmalonyl-CoA: 224.8
  Methoxymalonyl-CoA: 183.1
  2-Methylbutyryl-CoA: 153.8
  trans-1,2-CPDA: 148.1
  CHC-CoA: 110.9
  Propionyl-CoA: 106.3
  Malonyl-CoA: 101.2
  Isobutyryl-CoA: 71.8
  Benzoyl-CoA: 63.7
  Acetyl-CoA: 52.1
  3-Methylbutyryl-CoA: 29.8
  fatty_acid: 27.7
  inactive: 19.1

PKS_AT (3134..3431): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 224.5
  Ethylmalonyl-CoA: 221.8
  Methoxymalonyl-CoA: 182.2
  2-Methylbutyryl-CoA: 166.5
  trans-1,2-CPDA: 138.9
  Propionyl-CoA: 112.3
  CHC-CoA: 109.8
  Malonyl-CoA: 108.1
  Isobutyryl-CoA: 75.7
  Benzoyl-CoA: 60.6
  Acetyl-CoA: 57.4
  fatty_acid: 34.0
  3-Methylbutyryl-CoA: 30.9
  inactive: 28.5

scaP2: mmal - mmal - mal
PKS_AT (575..872): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 227.3
  Ethylmalonyl-CoA: 222.5
  Methoxymalonyl-CoA: 186.3
  2-Methylbutyryl-CoA: 159.9
  trans-1,2-CPDA: 144.1
  CHC-CoA: 117.7
  Propionyl-CoA: 109.6
  Malonyl-CoA: 109.3
  Isobutyryl-CoA: 83.7
  Benzoyl-CoA: 70.5
  Acetyl-CoA: 52.7
  3-Methylbutyryl-CoA: 42.3
  fatty_acid: 30.1
  inactive: 21.0

PKS_AT (2123..2420): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 227.9
  Ethylmalonyl-CoA: 223.5
  Methoxymalonyl-CoA: 186.6
  2-Methylbutyryl-CoA: 163.4
  trans-1,2-CPDA: 143.4
  CHC-CoA: 125.6
  Propionyl-CoA: 115.0
  Malonyl-CoA: 111.5
  Isobutyryl-CoA: 81.0
  Benzoyl-CoA: 65.8
  Acetyl-CoA: 53.4
  3-Methylbutyryl-CoA: 49.1
  fatty_acid: 33.0
  inactive: 26.9

PKS_AT (3665..3959): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 83.3%
  2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 229.2
  inactive: 122.5
  Methoxymalonyl-CoA: 67.0
  Methylmalonyl-CoA: 56.2
  Propionyl-CoA: 36.8
  2-Methylbutyryl-CoA: 25.7
  Ethylmalonyl-CoA: 23.2
  Isobutyryl-CoA: 21.5
  Acetyl-CoA: 20.2
  Benzoyl-CoA: 18.1
  fatty_acid: 14.5
  CHC-CoA: 8.0
  trans-1,2-CPDA: 0.0
  3-Methylbutyryl-CoA: 0.0

scaP3: mal - mal
PKS_AT (576..872): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 79.2%
  2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 210.1
  inactive: 108.3
  Methoxymalonyl-CoA: 59.8
  Methylmalonyl-CoA: 56.2
  Propionyl-CoA: 39.8
  2-Methylbutyryl-CoA: 35.9
  Isobutyryl-CoA: 29.3
  Benzoyl-CoA: 25.5
  Ethylmalonyl-CoA: 21.6
  Acetyl-CoA: 19.2
  CHC-CoA: 15.9
  fatty_acid: 15.7
  trans-1,2-CPDA: 14.6
  3-Methylbutyryl-CoA: 0.0

PKS_AT (2333..2625): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 91.7%
  inactive: 83.3%
  Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 214.7
  inactive: 111.7
  Methoxymalonyl-CoA: 57.9
  Methylmalonyl-CoA: 37.5
  Propionyl-CoA: 32.4
  Acetyl-CoA: 19.5
  Isobutyryl-CoA: 17.6
  2-Methylbutyryl-CoA: 11.0
  Ethylmalonyl-CoA: 10.1
  trans-1,2-CPDA: 0.0
  fatty_acid: 0.0
  CHC-CoA: 0.0
  Benzoyl-CoA: 0.0
  3-Methylbutyryl-CoA: 0.0

scaP4: mal - mal - mal
PKS_AT (595..888): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 215.2
  inactive: 118.5
  Methoxymalonyl-CoA: 71.3
  Methylmalonyl-CoA: 61.4
  Propionyl-CoA: 32.1
  Isobutyryl-CoA: 30.8
  Acetyl-CoA: 24.0
  Benzoyl-CoA: 20.6
  fatty_acid: 19.1
  Ethylmalonyl-CoA: 15.5
  CHC-CoA: 15.4
  2-Methylbutyryl-CoA: 10.6
  trans-1,2-CPDA: 7.3
  3-Methylbutyryl-CoA: 0.0

PKS_AT (2147..2444): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 100.0%
  inactive: 87.5%
  2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 221.8
  inactive: 131.0
  Methoxymalonyl-CoA: 62.9
  Methylmalonyl-CoA: 54.3
  Isobutyryl-CoA: 34.7
  Benzoyl-CoA: 30.0
  Propionyl-CoA: 27.7
  Ethylmalonyl-CoA: 22.5
  Acetyl-CoA: 22.0
  fatty_acid: 14.8
  trans-1,2-CPDA: 9.9
  2-Methylbutyryl-CoA: 7.7
  CHC-CoA: 6.2
  3-Methylbutyryl-CoA: 0.0

PKS_AT (4229..4522): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 83.3%
  Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 231.9
  inactive: 114.5
  Methoxymalonyl-CoA: 63.9
  Methylmalonyl-CoA: 58.1
  Propionyl-CoA: 36.3
  Isobutyryl-CoA: 24.8
  Ethylmalonyl-CoA: 24.0
  Benzoyl-CoA: 23.1
  Acetyl-CoA: 22.2
  2-Methylbutyryl-CoA: 20.3
  fatty_acid: 15.9
  CHC-CoA: 14.7
  trans-1,2-CPDA: 12.6
  3-Methylbutyryl-CoA: 0.0

scaP5: mal - mal - mal - mmal - mal
PKS_AT (572..866): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 100.0%
  inactive: 87.5%
  Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 228.5
  inactive: 137.5
  Methoxymalonyl-CoA: 65.7
  Methylmalonyl-CoA: 57.2
  Propionyl-CoA: 32.4
  Isobutyryl-CoA: 32.4
  Acetyl-CoA: 26.3
  Benzoyl-CoA: 25.9
  Ethylmalonyl-CoA: 19.0
  CHC-CoA: 15.5
  fatty_acid: 13.5
  2-Methylbutyryl-CoA: 11.9
  trans-1,2-CPDA: 9.6
  3-Methylbutyryl-CoA: 0.0

PKS_AT (2099..2392): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 79.2%
  2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 227.2
  inactive: 116.0
  Methoxymalonyl-CoA: 63.0
  Methylmalonyl-CoA: 53.3
  Propionyl-CoA: 32.5
  Isobutyryl-CoA: 26.9
  Ethylmalonyl-CoA: 23.5
  Benzoyl-CoA: 21.0
  Acetyl-CoA: 18.4
  CHC-CoA: 18.1
  fatty_acid: 14.5
  2-Methylbutyryl-CoA: 11.4
  trans-1,2-CPDA: 8.8
  3-Methylbutyryl-CoA: 0.0

PKS_AT (3556..3852): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 100.0%
  inactive: 87.5%
  Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 231.8
  inactive: 128.1
  Methoxymalonyl-CoA: 61.7
  Methylmalonyl-CoA: 57.1
  Propionyl-CoA: 32.6
  Isobutyryl-CoA: 29.4
  Acetyl-CoA: 22.3
  Benzoyl-CoA: 21.0
  Ethylmalonyl-CoA: 18.2
  CHC-CoA: 15.8
  fatty_acid: 13.1
  2-Methylbutyryl-CoA: 11.2
  trans-1,2-CPDA: 10.7
  3-Methylbutyryl-CoA: 0.0

PKS_AT (5259..5556): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 95.8%
  Methoxymalonyl-CoA: 87.5%
  Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 214.1
  Ethylmalonyl-CoA: 207.2
  Methoxymalonyl-CoA: 168.5
  2-Methylbutyryl-CoA: 161.5
  trans-1,2-CPDA: 135.8
  Propionyl-CoA: 111.8
  CHC-CoA: 105.1
  Malonyl-CoA: 83.9
  Isobutyryl-CoA: 79.4
  Benzoyl-CoA: 54.5
  Acetyl-CoA: 43.9
  3-Methylbutyryl-CoA: 41.2
  fatty_acid: 23.4
  inactive: 22.5

PKS_AT (6820..7121): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 83.3%
  2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 236.7
  inactive: 124.5
  Methoxymalonyl-CoA: 67.0
  Methylmalonyl-CoA: 62.0
  Propionyl-CoA: 39.2
  Isobutyryl-CoA: 34.1
  Ethylmalonyl-CoA: 29.2
  2-Methylbutyryl-CoA: 26.6
  Benzoyl-CoA: 25.2
  Acetyl-CoA: 21.6
  CHC-CoA: 16.0
  fatty_acid: 15.3
  trans-1,2-CPDA: 12.7
  3-Methylbutyryl-CoA: 0.0

scaP6: mal - mal - mal
PKS_AT (573..874): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 91.7%
  inactive: 83.3%
  2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 221.0
  inactive: 117.9
  Methoxymalonyl-CoA: 63.2
  Methylmalonyl-CoA: 50.6
  Propionyl-CoA: 30.5
  Isobutyryl-CoA: 29.5
  Benzoyl-CoA: 21.6
  Acetyl-CoA: 21.2
  Ethylmalonyl-CoA: 17.6
  fatty_acid: 11.9
  2-Methylbutyryl-CoA: 11.0
  trans-1,2-CPDA: 9.5
  CHC-CoA: 5.6
  3-Methylbutyryl-CoA: 0.0

PKS_AT (2045..2341): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 100.0%
  inactive: 87.5%
  Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 231.8
  inactive: 128.1
  Methoxymalonyl-CoA: 61.7
  Methylmalonyl-CoA: 57.1
  Propionyl-CoA: 32.6
  Isobutyryl-CoA: 29.4
  Acetyl-CoA: 22.3
  Benzoyl-CoA: 21.0
  Ethylmalonyl-CoA: 18.2
  CHC-CoA: 15.8
  fatty_acid: 13.1
  2-Methylbutyryl-CoA: 11.2
  trans-1,2-CPDA: 10.7
  3-Methylbutyryl-CoA: 0.0

PKS_AT (4163..4463): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 91.7%
  inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 208.5
  inactive: 102.0
  Methoxymalonyl-CoA: 66.3
  Methylmalonyl-CoA: 40.6
  Propionyl-CoA: 33.9
  Isobutyryl-CoA: 26.9
  Ethylmalonyl-CoA: 24.2
  Benzoyl-CoA: 17.8
  Acetyl-CoA: 17.7
  2-Methylbutyryl-CoA: 16.4
  fatty_acid: 12.5
  CHC-CoA: 2.7
  trans-1,2-CPDA: 0.0
  3-Methylbutyryl-CoA: 0.0

scaP7: mmal - mal
PKS_AT (570..867): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Malonyl-CoA: 91.7%
  Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 219.8
  Ethylmalonyl-CoA: 213.9
  Methoxymalonyl-CoA: 178.6
  2-Methylbutyryl-CoA: 163.9
  trans-1,2-CPDA: 136.8
  Propionyl-CoA: 122.2
  Malonyl-CoA: 107.4
  CHC-CoA: 104.4
  Isobutyryl-CoA: 87.1
  Benzoyl-CoA: 72.1
  Acetyl-CoA: 54.3
  3-Methylbutyryl-CoA: 44.1
  fatty_acid: 33.2
  inactive: 30.6

PKS_AT (2116..2409): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 100.0%
  inactive: 87.5%
  Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 231.2
  inactive: 127.3
  Methoxymalonyl-CoA: 66.3
  Methylmalonyl-CoA: 57.2
  Propionyl-CoA: 35.9
  Isobutyryl-CoA: 27.3
  Acetyl-CoA: 22.8
  Ethylmalonyl-CoA: 19.1
  Benzoyl-CoA: 18.6
  CHC-CoA: 15.9
  2-Methylbutyryl-CoA: 13.6
  fatty_acid: 13.4
  trans-1,2-CPDA: 10.8
  3-Methylbutyryl-CoA: 0.0

scaP8: AHBA - pk
CAL_domain (33..441): AHBA
minowa: AHBA

Prediction, score:
  AHBA: 324.7
  shikimic_acid: 245.8
  Acetyl-CoA: 12.0
  fatty_acid: 0.0
  NH2: 0.0

PKS_AT (1184..1480): pk
ATSignature: Methylmalonyl-CoA, Methoxymalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 95.8%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:
  Ethylmalonyl-CoA: 205.9
  Methylmalonyl-CoA: 198.4
  Methoxymalonyl-CoA: 167.2
  2-Methylbutyryl-CoA: 139.5
  trans-1,2-CPDA: 125.1
  CHC-CoA: 109.0
  Propionyl-CoA: 106.9
  Malonyl-CoA: 90.5
  Benzoyl-CoA: 52.0
  Isobutyryl-CoA: 48.4
  3-Methylbutyryl-CoA: 42.4
  Acetyl-CoA: 37.2
  fatty_acid: 30.8
  inactive: 16.5

scaG: Asn
Search NORINE for peptide: strict or relaxed
AMP-binding (415..815): Asn
nrpys: Asn

SVM prediction details:
  Predicted physicochemical class:
    hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
  Large clusters prediction:
    Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
  Small clusters prediction:
    asp,asn (Asp, Asn)
  Single AA prediction:
    asn (Asn)

Stachelhaus prediction details:
  Stachelhaus sequence:
    DVTNIIEIGK
  Nearest Stachelhaus code(s):
    Asn DGTKIGEIGK (56% 8Å match)
  Stachelhaus code match:
    70% (weak)

scaH5: pk
PKS_AT (31..280): pk
ATSignature: (unknown)
No matches above 50%
minowa: Isobutyryl-CoA

Prediction, score:
  Isobutyryl-CoA: 14.6
  trans-1,2-CPDA: 0.0
  inactive: 0.0
  fatty_acid: 0.0
  Propionyl-CoA: 0.0
  Methylmalonyl-CoA: 0.0
  Methoxymalonyl-CoA: 0.0
  Malonyl-CoA: 0.0
  Ethylmalonyl-CoA: 0.0
  CHC-CoA: 0.0
  Benzoyl-CoA: 0.0
  Acetyl-CoA: 0.0
  3-Methylbutyryl-CoA: 0.0
  2-Methylbutyryl-CoA: 0.0

scaJ: X
AMP-binding (97..309): X
nrpys: (unknown)

SVM prediction details:
  NOTE: uncertain match
  Predicted physicochemical class:
    hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
  Large clusters prediction:
    Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
  Small clusters prediction:
    N/A
  Single AA prediction:
    N/A

Stachelhaus prediction details:
  Stachelhaus sequence:
    ---EGGAFGK
  Nearest Stachelhaus code(s):
  Stachelhaus code match:
    0% (weak)

scaL: X
AMP-binding (240..427): X
nrpys: (unknown)

SVM prediction details:
  NOTE: uncertain match
  Predicted physicochemical class:
    hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
  Large clusters prediction:
    Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
  Small clusters prediction:
    N/A
  Single AA prediction:
    N/A

Stachelhaus prediction details:
  Stachelhaus sequence:
    ---FVIELPK
  Nearest Stachelhaus code(s):
  Stachelhaus code match:
    0% (weak)

Link to PARAS substrate predictor