BGC0001856: caniferolide A biosynthetic gene cluster from Streptomyces caniferus
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Location: 1 - 186,923 nt. (total: 186,923 nt).
This entry is originally from NCBI GenBank MK303577.1.

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Gene details
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General
Compounds
Genes
Polyketide
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001856
Short description caniferolide A biosynthetic gene cluster from Streptomyces caniferus
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • Polyketide (Macrolide)
Loci NCBI GenBank: MK303577.1
Compounds
  • caniferolide A
  • caniferolide B
  • caniferolide C
  • caniferolide D
Species Streptomyces caniferus [taxonomy]
References
Chemical products information
caniferolide A
(no structure information available)
C77H122O31S1
caniferolide B
(no structure information available)
C77H122O28
caniferolide C
(no structure information available)
C77H122O27
caniferolide D
(no structure information available)
C77H122O30S1
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • QBF51744.1
  • scaH1
 1 - 717 (-) hypothetical protein
  • Unknown
Sequence-based prediction
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  • QBF51745.1
  • scaH2
 1051 - 1758 (+) hypothetical protein
  • Unknown
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  • QBF51746.1
  • scaH3
 1891 - 2115 (-) hypothetical protein
  • Unknown
Sequence-based prediction
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  • QBF51747.1
  • scaPT
 2441 - 3160 (+) 4'-phosphopantetheinyl transferase
  • Unknown
Sequence-based prediction
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  • QBF51748.1
  • scaTR1
 3265 - 6141 (-) LuxR family transcriptional regulator
  • Regulation
Sequence-based prediction
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  • QBF51749.1
  • scaTR2
 7173 - 8018 (-) PAS-LuxR transcriptional regulator
  • Regulation
Sequence-based prediction
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  • QBF51750.1
  • scaTR3
 8365 - 11175 (-) LuxR family transcriptional regulator
  • Regulation
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  • QBF51751.1
  • scaTR4
 12219 - 12995 (+) GntR family transcriptional regulator
  • Regulation
Sequence-based prediction
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  • QBF51752.1
  • scaTR5
 13079 - 13711 (+) TetR family transcriptional regulator
  • Regulation
Sequence-based prediction
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  • QBF51753.1
  • scaDH1
 13842 - 15254 (-) NDP-hexose 2,3-dehydratase
  • Precursor biosynthesis
Sequence-based prediction
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  • QBF51754.1
  • scaP1
 15620 - 28261 (+) type I polyketide synthase
  • Scaffold biosynthesis
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  • QBF51755.1
  • scaP2
 28308 - 43157 (+) type I polyketide synthase
  • Scaffold biosynthesis
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  • QBF51756.1
  • scaP3
 43483 - 55263 (+) type I polyketide synthase
  • Scaffold biosynthesis
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  • QBF51757.1
  • scaP4
 55319 - 71188 (+) type I polyketide synthase
  • Scaffold biosynthesis
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  • QBF51758.1
  • scaP5
 71238 - 96572 (+) type I polyketide synthase
  • Scaffold biosynthesis
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  • QBF51759.1
  • scaP6
 96615 - 112340 (+) type I polyketide synthase
  • Scaffold biosynthesis
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  • QBF51760.1
  • scaP7
 112387 - 123315 (+) type I polyketide synthase
  • Scaffold biosynthesis
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  • QBF51761.1
  • scaF1
 123499 - 123702 (+) ferredoxin
  • Other enzymatic
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  • QBF51762.1
  • scaCP1
 123732 - 124946 (-) cytochrome P450
  • Other enzymatic
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  • QBF51763.1
  • scaGT1
 125364 - 126620 (+) glycosyltransferase
  • Tailoring (Glycosylation)
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  • QBF51764.1
  • scaGS
 126678 - 127754 (+) glucose-1-phosphate thymidyltransferase
  • Precursor biosynthesis
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  • QBF51765.1
  • scaDH2
 127856 - 128857 (+) NDP-glucose-4,6-dehydratase
  • Precursor biosynthesis
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  • QBF51766.1
  • scaR1
 128893 - 129864 (+) NAD(P)H-dependent ketoreductase
  • Precursor biosynthesis
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  • QBF51767.1
  • scaGT2
 129967 - 131193 (+) glycosyltransferase
  • Tailoring (Glycosylation)
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  • QBF51768.1
  • scaDH3
 131283 - 132584 (-) PMP-dependent NDP-4-keto-hexose 3-dehydratase
  • Precursor biosynthesis
Sequence-based prediction
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  • QBF51769.1
  • scaP8
 133114 - 140286 (+) type I polyketide synthase
  • Scaffold biosynthesis
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  • QBF51770.1
  • scaS
 140321 - 141352 (+) 3-oxoacyl-ACP synthase
  • Scaffold biosynthesis
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  • QBF51771.1
  • scaA
 141448 - 141885 (+) cold-shock domain-containing protein
  • Unknown
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  • QBF51772.1
  • scaT1
 142323 - 143174 (-) ABC transporter permease component
  • Transport
Sequence-based prediction
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  • QBF51773.1
  • scaT2
 143201 - 143983 (-) ABC transporter permease component
  • Transport
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  • QBF51774.1
  • scaR2
 144167 - 145147 (-) NAD(P)H-dependent ketoreductase
  • Precursor biosynthesis
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  • QBF51775.1
  • scaE
 145140 - 145760 (-) dTDP-4-keto-2,6-dideoxy-3,5-epimerase
  • Precursor biosynthesis
Sequence-based prediction
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  • QBF51776.1
  • scaCM
 146066 - 147298 (-) NDP-hexose-3-C-methyltransferase
  • Precursor biosynthesis
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  • QBF51777.1
  • scaH4
 147727 - 148458 (+) hypothetical protein
  • Unknown
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  • QBF51778.1
  • scaST
 148720 - 150009 (+) sulfotransferase
  • Tailoring (Sulfation)
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  • QBF51779.1
  • scaSK
 150011 - 150616 (+) adenylyl-sulfate kinase
  • Other enzymatic
Sequence-based prediction
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  • QBF51780.1
  • scaO1
 150674 - 150844 (+) truncated flavin-dependent oxidoreductase
  • Other enzymatic
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  • QBF51781.1
  • scaO2
 150895 - 151689 (+) flavin-dependent oxidoreductase
  • Other enzymatic
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  • QBF51782.1
  • scaB
 152120 - 152332 (-) truncated IS481 family transposase
  • Unknown
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  • QBF51783.1
  • scaC
 152379 - 153557 (-) efflux permease, MFS family
  • Transport
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  • QBF51784.1
  • scaD
 153825 - 154376 (-) acetyltransferase
  • Tailoring (Acetylation)
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  • QBF51785.1
  • scaF
 154446 - 155492 (+) LD-carboxypeptidase
  • Tailoring (Decarboxylation)
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  • QBF51786.1
  • scaG
 155569 - 158616 (-) Nrps
  • Scaffold biosynthesis
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  • QBF51787.1
  • scaI
 158814 - 168908 (-) IPT/TIG domain-containing protein
  • Unknown
Sequence-based prediction
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  • QBF51788.1
  • scaH5
 169321 - 170304 (-) hypothetical protein
  • Unknown
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  • QBF51789.1
  • scaH6
 170301 - 170702 (-) hypothetical protein
  • Unknown
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  • QBF51790.1
  • scaT
 171023 - 171793 (+) thioesterase
  • Scaffold biosynthesis
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  • QBF51791.1
  • scaM1
 171841 - 172746 (+) menaquinone biosynthesis
  • Precursor biosynthesis
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  • QBF51792.1
  • scaM2
 172748 - 174016 (+) menaquinone biosynthesis
  • Precursor biosynthesis
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  • QBF51793.1
  • scaF2
 174253 - 174558 (-) ferredoxin
  • Other enzymatic
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  • QBF51794.1
  • scaM3
 174619 - 175788 (-) menaquinone biosynthesis
  • Precursor biosynthesis
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  • QBF51795.1
  • scaJ
 175848 - 176930 (-) putative arylcarboxylate reductase
  • Other enzymatic
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  • QBF51796.1
  • scaM4
 177175 - 178038 (+) menaquinone biosynthesis
  • Precursor biosynthesis
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  • QBF51797.1
  • scaTR6
 178099 - 178674 (-) TetR family transcriptional regulator
  • Transport
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  • QBF51798.1
  • scaH7
 178769 - 179215 (+) hypothetical protein
  • Unknown
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  • QBF51799.1
  • scaR3
 179338 - 180417 (-) NDP-hexose-3-ketoreductase
  • Precursor biosynthesis
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  • QBF51800.1
  • scaCP2
 180530 - 181747 (-) cytochrome P450
  • Other enzymatic
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  • QBF51801.1
  • scaK
 182281 - 183870 (-) carboxyl-transferase
  • Tailoring (Carboxylation)
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  • QBF51802.1
  • scaL
 183926 - 185251 (-) ATP-dependent acyl-CoA synthetase
  • Other enzymatic
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  • QBF51803.1
  • scaN
 185641 - 186549 (+) putative carboxylesterase
  • Unknown
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  • QBF51804.1
  • scaH8
 186504 - 186689 (-) hypothetical protein
  • Unknown
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  • QBF51805.1
  • scaH9
 186707 - 186922 (-) hypothetical protein
  • Unknown
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Polyketide-specific information
Subclass Macrolide
Starter unit Methylmalonyl-CoA
Cyclic? yes
Release type
  • Macrolactonization
Polyketide-synthases
Genes Properties Modules
scaP6
+
scaP3
+
scaP4
+
scaP2
+
scaP8
+
scaP5
+
scaP7
+
scaP1 		Synthase subclass: Modular type I
Module 1
Specificity: Methylmalonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: scaP1
Core domains: Ketosynthase, Acyltransferase, Thiolation (ACP/PCP)
Module 2
Specificity: Methylmalonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: scaP1
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 3
Specificity: Methylmalonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: scaP1
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 4
Specificity: Methylmalonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: scaP2
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 5
Specificity: Methylmalonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: scaP2
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 6
Specificity: Malonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: scaP2
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 7
Specificity: Malonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: scaP3
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 8
Specificity: Malonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: scaP3
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Enoylreductase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 9
Specificity: Malonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: scaP4
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Enoylreductase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 10
Specificity: Malonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: scaP4
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 11
Specificity: Malonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: scaP4
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 12
Specificity: Malonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: scaP5
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 13
Specificity: Malonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: scaP5
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 14
Specificity: Malonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: scaP5
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Thiolation (ACP/PCP)
Module 15
Specificity: Methylmalonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: scaP5
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 16
Specificity: Malonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: scaP5
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Enoylreductase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 17
Specificity: Malonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: scaP6
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 18
Specificity: Malonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: scaP6
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Enoylreductase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 19
Specificity: Malonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: scaP6
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 20
Specificity: Methylmalonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: scaP7
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 21
Specificity: Malonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: scaP7
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP), Thioesterase
KR-domain stereochemistry: D-OH
Module 22
Specificity: Unknown/3-methyl-1/ 4-dihydroxy-6-naphthoic acid
Evidence for specificity: Sequence-based prediction
Genes: scaP8
Core domains: CoA-ligase, Thiolation (ACP/PCP)
Module 23
Specificity: Methylmalonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: scaP8
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Annotation changelog
MIBiG version Submitter Notes
2.0
  • Hidden contributor (ID: AKJHE6WY6NZKFMVL6KWSMX5H, no GDPR consent given).
  • Submitted
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Corrected PKS module numbers
  • Updated bioactivity data
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
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