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Overview
1.1

### Identified secondary metabolite regions using strictness 'relaxed'

BGC0001877 (Streptomyces sp. ISID311)

Region | Type | From | To | Similarity Confidence | Most similar known cluster
--- | --- | --- | --- | --- | ---
Region 1 | NRPS-like,prodigiosin,benzoxazole,T1PKS,oligosaccharide,butyrolactone,transAT-PKS | 1 | 198,913 | High | cyphomycin | PKS

BGC0001877 - Region 1 - NRPS-like,T1PKS,benzoxazole,butyrolactone,oligosaccharide,prodigiosin,transAT-PKS
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
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Location: 1 - 198,913 nt. (total: 198,913 nt) Show pHMM detection rules used
Region on contig edge.
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
prodigiosin: (PPDK_N and AMP-binding and PP-binding and PKS_KS)
benzoxazole: (ksIII and AMP-binding and PP-binding and Amidohydro_1)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
oligosaccharide: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
butyrolactone: (repeatable(3, [DUF1205, Glyco_transf_28, Glycos_transf_1, Glycos_transf_2, MGT]) and minscore(MGT, 150))
transAT-PKS: AfsA

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
MIBiG
Tailoring
MIBiG comparison
KnownClusterBlast
NRPS/PKS modules
NRPS/PKS domains
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
External annotation
This annotation was made externally by 'MIBiG' and not by antiSMASH

Name |  | MIBiG
Version |  | 4.0alpha

Type |  | Subregion
Location |  | 1 - 198,914
Label |  | BGC0001877

Tailoring functions
Tailoring enzymes detected in this region
Oxidoreductases: dehydrogenase oxygenase
FS847_01830: cytochrome P450

smcogs
  SMCOG1034: cytochrome P450

FS847_01945: cytochrome P450

smcogs
  SMCOG1007: cytochrome P450

FS847_01980: short-chain dehydrogenase/reductase SDR

smcogs
  SMCOG1001: short-chain dehydrogenase/reductase SDR

FS847_02010: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs
  SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

FS847_02020: cytochrome P450

smcogs
  SMCOG1034: cytochrome P450

Transferases: PPTase acylase aminotransferase glycosyltransferase sulfotransferase transferase
FS847_01755: Glycosylation

MITE
  MITE0000033Glycosylation (identity: 60.80%)
smcogs
  SMCOG1102: glycosyltransferase

FS847_01765: Sulfotransferase domain

extras
  Sulfotransfer_3: Sulfotransferase domain

FS847_01880: glycosyltransferase, MGT family

smcogs
  SMCOG1062: glycosyltransferase, MGT family

FS847_01915: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs
  SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

FS847_01920: glycosyltransferase, MGT family

smcogs
  SMCOG1062: glycosyltransferase, MGT family

FS847_01935: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs
  SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

FS847_01940: glycosyltransferase, MGT family

smcogs
  SMCOG1062: glycosyltransferase, MGT family

FS847_01970: malonyl CoA-acyl carrier protein transacylase

smcogs
  SMCOG1021: malonyl CoA-acyl carrier protein transacylase

FS847_02035: malonyl CoA-acyl carrier protein transacylase

smcogs
  SMCOG1021: malonyl CoA-acyl carrier protein transacylase

FS847_02070: 4'-phosphopantetheinyl transferase

smcogs
  SMCOG1060: 4'-phosphopantetheinyl transferase

Hydrolases: hydrolase
FS847_01745: beta-lactamase

smcogs
  SMCOG1053: beta-lactamase

Lyases: dehydratase
FS847_01785: NAD-dependent epimerase/dehydratase

smcogs
  SMCOG1010: NAD-dependent epimerase/dehydratase

FS847_01845: NAD-dependent epimerase/dehydratase

smcogs
  SMCOG1010: NAD-dependent epimerase/dehydratase

FS847_01925: NAD-dependent epimerase/dehydratase

smcogs
  SMCOG1010: NAD-dependent epimerase/dehydratase

FS847_01930: NAD-dependent epimerase/dehydratase

smcogs
  SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase
FS847_01740: AMP-dependent synthetase and ligase

smcogs
  SMCOG1002: AMP-dependent synthetase and ligase

FS847_01905: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs
  SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

FS847_01955: Beta-ketoacyl synthase

smcogs
  SMCOG1093: Beta-ketoacyl synthase

FS847_01960: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

FS847_01965: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

FS847_01985: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

FS847_01990: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

FS847_01995: Beta-ketoacyl synthase

smcogs
  SMCOG1093: Beta-ketoacyl synthase

FS847_02030: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters
Shows areas that are similar to the current region to a reference database.
Mouseover a score cell in the table to get a breakdown of how the score was calculated.The MIBiG database.
Click on an accession to open that entry in the MIBiG database.
Analysis type:

Reference | NRPS-like | prodigiosin | benzoxazole | T1PKS | NRPS-like | oligosaccharide | butyrolactone | transAT-PKS | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | ---
BGC0001877.4 |  |  |  |  |  |  |  |  | 2.89 | PKS | cyphomycin | Streptomyces sp. ISID311
BGC0001276.3 |  |  |  |  |  |  |  |  | 2.24 | PKS | 6-methylsalicyclic acid | Aspergillus terreus
BGC0001273.3 |  |  |  |  |  |  |  |  | 2.21 | PKS | asperlactone | Aspergillus ochraceus
BGC0001160.5 |  |  |  |  |  |  |  |  | 2.10 | PKS | 1-nonadecene, (14Z)-1,14-nonadecadiene | Synechococcus sp. PCC 7002
BGC0001164.3 |  |  |  |  |  |  |  |  | 2.09 | PKS | 1-heptadecene | Cyanothece sp. PCC 7822
BGC0002191.2 |  |  |  |  |  |  |  |  | 2.00 | PKS | prolipyrone B, gibepyrone D | Fusarium graminearum PH-1
BGC0002192.3 |  |  |  |  |  |  |  |  | 1.82 | PKS | FR901512 | fungal sp. No.14919
BGC0000037.4 |  |  |  |  |  |  |  |  | 1.78 | PKS | cichorine | Aspergillus nidulans FGSC A4
BGC0001458.4 |  |  |  |  |  |  |  |  | 1.49 | other (aminocoumarin) | salinipostin G, Salinipostin G, Salinipostin A, Salinipostin B, Salinipostin C, Salinipostin D, Salinipostin E, Salinipostin F, Salinipostin H, Salinipostin I, Salinipostin J, Salinipostin K | Salinispora tropica CNB-440
BGC0000850.5 |  |  |  |  |  |  |  |  | 1.42 | other (other) | γ-butyrolactone | Streptomyces acidiscabies

Reference | Aggregated | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0001877.4 |  | 0.99 | PKS | cyphomycin | Streptomyces sp. ISID311
BGC0001273.3 |  | 0.76 | PKS | asperlactone | Aspergillus ochraceus
BGC0001276.3 |  | 0.75 | PKS | 6-methylsalicyclic acid | Aspergillus terreus
BGC0001244.3 |  | 0.75 | PKS | (-)-Mellein | Parastagonospora nodorum
BGC0001164.3 |  | 0.71 | PKS | 1-heptadecene | Cyanothece sp. PCC 7822
BGC0001249.5 |  | 0.71 | NRPS | dimethylcoprogen | Alternaria alternata
BGC0001160.5 |  | 0.70 | PKS | 1-nonadecene, (14Z)-1,14-nonadecadiene | Synechococcus sp. PCC 7002
BGC0002191.2 |  | 0.68 | PKS | prolipyrone B, gibepyrone D | Fusarium graminearum PH-1
BGC0000041.3 |  | 0.67 | PKS | oronofacic acid | Pseudomonas syringae
BGC0001257.3 |  | 0.67 | PKS | 1,3,6,8-tetrahydroxynaphthalene | Nodulisporium sp. ATCC74245

Similar known clusters from MIBiG 4.0
Shows clusters from the MIBiG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Detailed help and explanations are available here.
Click on reference genes to show details of similarities to genes within the current region.
Double click on a reference drawing to reverse the display of the genes.
Click on an accession to open that entry in the MIBiG database.
Location:
Identifier
Identity
Coverage
Module view
Shows module structures for each candidate cluster in NRPS and PKS regions.
Genes are shown in predicted order, and are only present when containing at least one complete module.
A domain glossary is available here, and an explanation of the visualisation is available here.
Legend
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A domain glossary is available here, and an explanation of the visualisation is available here.
Selected features only
Show module domains
NRPS/PKS products
NRPS/PKS substrates
Predicted core structure(s)
Shows estimated product structure and polymer for each candidate cluster in the region. To show the product, click on the expander or the candidate cluster feature drawn in the overview.
For candidate cluster 1, location 0 - 198913:
Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:
  (Me-mal - pk - Me-ohmal) + (Me-ohmal - pk - ccmal) + (ccmal - redmal) + (ohmal - redmal - ohmal) + (Me-ohmal - redmal) + (ohmal - redmal - ohmal) + (Me-ohmal - ccmal) + (AHBA - pk) + (Gln) + (Asn)

Direct lookup in NORINE database: strict or relaxed
Link to NORINE database query form
NRPS/PKS substrate predictions
Shows the predicted substrates for each adenylation domain and acyltransferase within genes. Each gene prediction can be expanded to view detailed predictions of each domain. Each prediction can be expanded to view the predictions by tool (and, for some tools, further expanded for extra details).
FS847_01740: Asn
Search NORINE for peptide: strict or relaxed
AMP-binding (415..819): Asn
nrpys: Asn

SVM prediction details:
  Predicted physicochemical class:
    hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
  Large clusters prediction:
    Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
  Small clusters prediction:
    asp,asn (Asp, Asn)
  Single AA prediction:
    asn (Asn)

Stachelhaus prediction details:
  Stachelhaus sequence:
    DVTNIIEIGK
  Nearest Stachelhaus code(s):
    Asn DGTKIGEIGK (56% 8Å match)
  Stachelhaus code match:
    70% (weak)

FS847_01805: Gln
Search NORINE for peptide: strict or relaxed
AMP-binding (19..417): Gln
nrpys: Gln

SVM prediction details:
  Predicted physicochemical class:
    hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
  Large clusters prediction:
    Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
  Small clusters prediction:
    N/A
  Single AA prediction:
    N/A

Stachelhaus prediction details:
  Stachelhaus sequence:
    DAWQFGLINK
  Nearest Stachelhaus code(s):
    Gln DAWQFGLINK (94% 8Å match)
  Stachelhaus code match:
    100% (strong)

FS847_01910: AHBA - pk
CAL_domain (33..441): AHBA
minowa: AHBA

Prediction, score:
  AHBA: 325.3
  shikimic_acid: 259.1
  Acetyl-CoA: 16.7
  NH2: 0.1
  fatty_acid: 0.0

PKS_AT (1178..1474): pk
ATSignature: Methylmalonyl-CoA, Methoxymalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 91.7%
  Methoxymalonyl-CoA: 91.7%
  Ethylmalonyl-CoA: 83.3%

minowa: Ethylmalonyl-CoA

Prediction, score:
  Ethylmalonyl-CoA: 203.0
  Methylmalonyl-CoA: 194.2
  Methoxymalonyl-CoA: 159.1
  2-Methylbutyryl-CoA: 140.0
  trans-1,2-CPDA: 125.0
  Propionyl-CoA: 111.2
  CHC-CoA: 105.9
  Malonyl-CoA: 79.9
  Benzoyl-CoA: 50.3
  Isobutyryl-CoA: 45.9
  Acetyl-CoA: 40.9
  fatty_acid: 32.7
  3-Methylbutyryl-CoA: 31.6
  inactive: 17.5

FS847_01955: mmal - mal
PKS_AT (569..865): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Malonyl-CoA: 91.7%
  Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 221.8
  Ethylmalonyl-CoA: 215.3
  Methoxymalonyl-CoA: 181.7
  2-Methylbutyryl-CoA: 161.3
  trans-1,2-CPDA: 142.1
  Propionyl-CoA: 121.9
  Malonyl-CoA: 111.0
  CHC-CoA: 100.1
  Isobutyryl-CoA: 87.9
  Benzoyl-CoA: 73.5
  Acetyl-CoA: 50.5
  3-Methylbutyryl-CoA: 45.7
  fatty_acid: 35.7
  inactive: 35.4

PKS_AT (2115..2408): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 83.3%
  2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 234.7
  inactive: 128.0
  Methoxymalonyl-CoA: 60.9
  Methylmalonyl-CoA: 54.4
  Propionyl-CoA: 36.3
  Isobutyryl-CoA: 33.1
  Ethylmalonyl-CoA: 27.6
  Acetyl-CoA: 26.3
  Benzoyl-CoA: 25.6
  2-Methylbutyryl-CoA: 16.0
  CHC-CoA: 15.5
  fatty_acid: 12.9
  trans-1,2-CPDA: 8.8
  3-Methylbutyryl-CoA: 0.0

FS847_01960: mal - mal - mal
PKS_AT (572..875): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 83.3%
  2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 231.2
  inactive: 120.3
  Methoxymalonyl-CoA: 55.8
  Methylmalonyl-CoA: 45.4
  Propionyl-CoA: 28.9
  Isobutyryl-CoA: 27.0
  Acetyl-CoA: 24.8
  Benzoyl-CoA: 15.8
  CHC-CoA: 13.5
  Ethylmalonyl-CoA: 13.2
  2-Methylbutyryl-CoA: 10.3
  fatty_acid: 8.7
  trans-1,2-CPDA: 0.0
  3-Methylbutyryl-CoA: 0.0

PKS_AT (2047..2347): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 83.3%
  2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 241.2
  inactive: 129.7
  Methoxymalonyl-CoA: 63.8
  Methylmalonyl-CoA: 57.5
  Propionyl-CoA: 32.2
  Isobutyryl-CoA: 30.4
  Acetyl-CoA: 26.5
  Benzoyl-CoA: 23.0
  Ethylmalonyl-CoA: 18.7
  CHC-CoA: 15.3
  fatty_acid: 12.7
  2-Methylbutyryl-CoA: 12.2
  trans-1,2-CPDA: 9.2
  3-Methylbutyryl-CoA: 0.0

PKS_AT (4174..4475): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 91.7%
  inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 204.6
  inactive: 96.3
  Methoxymalonyl-CoA: 63.5
  Methylmalonyl-CoA: 41.1
  Isobutyryl-CoA: 37.4
  Propionyl-CoA: 30.3
  Benzoyl-CoA: 27.9
  Ethylmalonyl-CoA: 22.5
  Acetyl-CoA: 21.8
  fatty_acid: 11.3
  2-Methylbutyryl-CoA: 10.5
  CHC-CoA: 2.6
  trans-1,2-CPDA: 0.0
  3-Methylbutyryl-CoA: 0.0

FS847_01965: mmal - mal
PKS_AT (840..1137): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 95.8%
  Methoxymalonyl-CoA: 87.5%
  Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 215.7
  Ethylmalonyl-CoA: 209.3
  Methoxymalonyl-CoA: 171.9
  2-Methylbutyryl-CoA: 168.7
  trans-1,2-CPDA: 149.4
  Propionyl-CoA: 115.9
  CHC-CoA: 98.6
  Malonyl-CoA: 90.5
  Isobutyryl-CoA: 80.5
  Benzoyl-CoA: 58.6
  Acetyl-CoA: 51.8
  3-Methylbutyryl-CoA: 33.2
  fatty_acid: 27.1
  inactive: 17.5

PKS_AT (2421..2724): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 83.3%
  2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 236.0
  inactive: 130.8
  Methoxymalonyl-CoA: 66.5
  Methylmalonyl-CoA: 57.9
  Isobutyryl-CoA: 37.6
  Propionyl-CoA: 36.0
  Benzoyl-CoA: 24.6
  Acetyl-CoA: 22.2
  Ethylmalonyl-CoA: 20.7
  CHC-CoA: 15.9
  2-Methylbutyryl-CoA: 14.6
  fatty_acid: 13.2
  trans-1,2-CPDA: 9.1
  3-Methylbutyryl-CoA: 0.0

FS847_01970: mal
PKS_AT (0..285): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 87.5%
  inactive: 79.2%
  2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 224.5
  inactive: 123.3
  Methoxymalonyl-CoA: 63.0
  Methylmalonyl-CoA: 54.0
  Propionyl-CoA: 38.5
  Isobutyryl-CoA: 30.8
  Ethylmalonyl-CoA: 27.3
  Acetyl-CoA: 26.9
  Benzoyl-CoA: 23.8
  CHC-CoA: 16.8
  fatty_acid: 14.5
  2-Methylbutyryl-CoA: 13.8
  trans-1,2-CPDA: 9.0
  3-Methylbutyryl-CoA: 0.0

FS847_01985: mal
PKS_AT (572..867): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 83.3%
  2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 227.7
  inactive: 121.8
  Methoxymalonyl-CoA: 58.1
  Methylmalonyl-CoA: 50.1
  Propionyl-CoA: 34.7
  Isobutyryl-CoA: 29.8
  Acetyl-CoA: 24.1
  Ethylmalonyl-CoA: 22.2
  Benzoyl-CoA: 22.2
  CHC-CoA: 12.9
  2-Methylbutyryl-CoA: 12.1
  fatty_acid: 9.4
  trans-1,2-CPDA: 0.0
  3-Methylbutyryl-CoA: 0.0

FS847_01990: mal - mal - mal
PKS_AT (577..871): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 79.2%
  2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 214.2
  inactive: 116.2
  Methoxymalonyl-CoA: 70.0
  Methylmalonyl-CoA: 56.9
  Propionyl-CoA: 33.2
  Acetyl-CoA: 22.6
  Isobutyryl-CoA: 17.0
  Benzoyl-CoA: 13.5
  fatty_acid: 12.0
  Ethylmalonyl-CoA: 11.4
  2-Methylbutyryl-CoA: 9.7
  CHC-CoA: 8.5
  trans-1,2-CPDA: 7.4
  3-Methylbutyryl-CoA: 0.0

PKS_AT (2124..2421): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 79.2%
  2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 238.0
  inactive: 125.8
  Methoxymalonyl-CoA: 65.4
  Methylmalonyl-CoA: 58.5
  Propionyl-CoA: 39.5
  Isobutyryl-CoA: 32.3
  Ethylmalonyl-CoA: 28.9
  Acetyl-CoA: 26.3
  Benzoyl-CoA: 26.2
  CHC-CoA: 17.5
  2-Methylbutyryl-CoA: 12.7
  fatty_acid: 12.6
  trans-1,2-CPDA: 9.2
  3-Methylbutyryl-CoA: 0.0

PKS_AT (4191..4485): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 100.0%
  inactive: 79.2%
  2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 238.8
  inactive: 120.0
  Methoxymalonyl-CoA: 62.7
  Methylmalonyl-CoA: 55.0
  Propionyl-CoA: 34.2
  Isobutyryl-CoA: 22.6
  Acetyl-CoA: 22.0
  Benzoyl-CoA: 17.7
  Ethylmalonyl-CoA: 17.0
  CHC-CoA: 13.4
  2-Methylbutyryl-CoA: 11.8
  fatty_acid: 11.0
  trans-1,2-CPDA: 0.0
  3-Methylbutyryl-CoA: 0.0

FS847_01995: mal - mal
PKS_AT (580..876): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 79.2%
  2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 209.5
  inactive: 111.3
  Methoxymalonyl-CoA: 66.8
  Methylmalonyl-CoA: 58.2
  Propionyl-CoA: 35.0
  Isobutyryl-CoA: 31.7
  Benzoyl-CoA: 31.5
  2-Methylbutyryl-CoA: 29.6
  Ethylmalonyl-CoA: 27.8
  Acetyl-CoA: 23.7
  fatty_acid: 15.9
  CHC-CoA: 15.9
  trans-1,2-CPDA: 15.2
  3-Methylbutyryl-CoA: 0.0

PKS_AT (2358..2651): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 91.7%
  inactive: 79.2%
  2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 208.5
  inactive: 118.4
  Methoxymalonyl-CoA: 62.1
  Methylmalonyl-CoA: 39.8
  Propionyl-CoA: 28.1
  Acetyl-CoA: 19.6
  Isobutyryl-CoA: 18.1
  Ethylmalonyl-CoA: 7.4
  2-Methylbutyryl-CoA: 6.9
  trans-1,2-CPDA: 0.0
  fatty_acid: 0.0
  CHC-CoA: 0.0
  Benzoyl-CoA: 0.0
  3-Methylbutyryl-CoA: 0.0

FS847_02030: mmal - pk - mal
PKS_AT (574..871): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 229.6
  Ethylmalonyl-CoA: 228.3
  Methoxymalonyl-CoA: 185.8
  trans-1,2-CPDA: 159.4
  2-Methylbutyryl-CoA: 158.4
  CHC-CoA: 121.0
  Propionyl-CoA: 116.8
  Malonyl-CoA: 110.9
  Isobutyryl-CoA: 74.2
  Benzoyl-CoA: 69.9
  Acetyl-CoA: 56.9
  3-Methylbutyryl-CoA: 36.2
  fatty_acid: 29.6
  inactive: 26.1

PKS_AT (2119..2416): pk
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:
  Ethylmalonyl-CoA: 233.4
  Methylmalonyl-CoA: 232.4
  Methoxymalonyl-CoA: 188.4
  2-Methylbutyryl-CoA: 162.6
  trans-1,2-CPDA: 151.8
  CHC-CoA: 122.9
  Propionyl-CoA: 116.7
  Malonyl-CoA: 110.0
  Isobutyryl-CoA: 82.5
  Benzoyl-CoA: 70.7
  Acetyl-CoA: 52.9
  3-Methylbutyryl-CoA: 44.3
  fatty_acid: 34.6
  inactive: 27.9

PKS_AT (3663..3957): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 83.3%
  2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 230.7
  inactive: 115.9
  Methoxymalonyl-CoA: 66.9
  Methylmalonyl-CoA: 55.4
  Propionyl-CoA: 34.8
  2-Methylbutyryl-CoA: 29.9
  Isobutyryl-CoA: 26.6
  Ethylmalonyl-CoA: 24.1
  Acetyl-CoA: 20.6
  fatty_acid: 18.5
  Benzoyl-CoA: 18.5
  CHC-CoA: 10.8
  trans-1,2-CPDA: 0.0
  3-Methylbutyryl-CoA: 0.0

FS847_02035: mmal - pk - mmal
PKS_AT (551..848): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 222.1
  Ethylmalonyl-CoA: 218.9
  Methoxymalonyl-CoA: 173.2
  2-Methylbutyryl-CoA: 162.5
  trans-1,2-CPDA: 138.0
  Propionyl-CoA: 119.3
  CHC-CoA: 113.9
  Malonyl-CoA: 110.1
  Isobutyryl-CoA: 65.1
  Benzoyl-CoA: 55.5
  Acetyl-CoA: 55.4
  fatty_acid: 25.7
  inactive: 24.8
  3-Methylbutyryl-CoA: 17.1

PKS_AT (1584..1881): pk
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:
  Ethylmalonyl-CoA: 229.4
  Methylmalonyl-CoA: 229.2
  Methoxymalonyl-CoA: 186.7
  2-Methylbutyryl-CoA: 158.6
  trans-1,2-CPDA: 144.6
  CHC-CoA: 118.6
  Propionyl-CoA: 113.1
  Malonyl-CoA: 111.6
  Isobutyryl-CoA: 78.2
  Benzoyl-CoA: 70.7
  Acetyl-CoA: 53.5
  fatty_acid: 29.7
  3-Methylbutyryl-CoA: 29.5
  inactive: 28.2

PKS_AT (3165..3461): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 231.5
  Ethylmalonyl-CoA: 229.4
  Methoxymalonyl-CoA: 185.0
  2-Methylbutyryl-CoA: 166.7
  trans-1,2-CPDA: 137.6
  CHC-CoA: 120.8
  Malonyl-CoA: 114.1
  Propionyl-CoA: 112.6
  Isobutyryl-CoA: 81.4
  Benzoyl-CoA: 67.5
  Acetyl-CoA: 57.0
  fatty_acid: 37.0
  3-Methylbutyryl-CoA: 30.3
  inactive: 24.3

Link to PARAS substrate predictor