LOCUS       BGC0001889              5090 bp    DNA     linear   BCT 28-JAN-2014
DEFINITION  Thermobifida fusca YX, complete genome.
ACCESSION   BGC0001889
VERSION     BGC0001889
KEYWORDS    .
SOURCE      Thermobifida fusca YX
  ORGANISM  Thermobifida fusca YX
            Bacteria; Actinobacteria; Streptosporangiales; Nocardiopsaceae;
            Thermobifida.
COMMENT     URL -- http://www.jgi.doe.gov
            Source DNA and bacteria available from David Wilson
            (dbw3@cornell.edu)
            Whole genome sequencing and draft assembly at JGI-PGF
            Finishing done by JGI-PGF
            Quality assurance done by JGI-Stanford
            Annotation done by JGI-ORNL and JGI-PGF
            Contacts: Paul Richardson (microbes@cuba.jgi-psf.org)
            Finished microbial genomes have been curated to close all gaps with
            greater than 98% coverage of at least two independent clones. Each
            base pair has a minimum q (quality) value of 30 and the total error
            rate is less than one per 50000.
            ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-08 05:16:11
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 1333038
            Orig. end    :: 1338128
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     subregion       1..5090
                     /aStool="mibig"
                     /contig_edge="False"
                     /label="RiPP"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..5090
                     /contig_edge="False"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(2..1738)
                     /locus_tag="Tfu_1142"
     CDS             complement(2..1738)
                     /codon_start=1
                     /db_xref="InterPro:IPR001687"
                     /db_xref="InterPro:IPR001969"
                     /db_xref="InterPro:IPR003439"
                     /db_xref="InterPro:IPR003593"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 350.4; E-value:
                     3.2e-106)"
                     /gene_kind="transport"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /locus_tag="Tfu_1142"
                     /product="ABC-type multidrug transport system ATPase and
                     permease components"
                     /protein_id="AAZ55180.1"
                     /transl_table=11
                     /translation="MPLSPPRSLRLLVAHLWPHRRAVAFGALLGLLGGIGTLAEPLVAM
                     AVVDALGEGSPLGWLLALLTVLVVGGAALAGLSSYVLHRTAESMVAAARRRLVSHILLL
                     RVPELDRLKPGDLLSRVTSDTTYIRSAAGQALVDSGSALLVAIGSIVLMAWIDLPLLLV
                     CLAVIGVIGVGSAVMMPPIRRANERSQRAVGEVGALVERALGAFRTLKASSAERREISA
                     AKAAVRTAWREGVRSAAWTAATNVAVVVTSQAAFLVVLGAGGARVAMGAIDVSELIAFL
                     LYLMRLTGFVAQLAQAVSSLQSGLAAMRRIAEVEQLPVEHIGVPPRRTPAATSAASVSF
                     TGVSFRYREDGPWTLRNVTLDVPAGGLTALVGPSGAGKTTLFSLVERFYDPHEGVVAID
                     GVDVRDIPLVRLRSMIGYVEQDAPILAGTLRDNLCFAAPHADEEEIRRVVELTRLTSLV
                     ERLPDGLDTQVGHRGTTLSGGERQRVAIARALLRRPRLLLLDEATSQLDATNETALRDV
                     VVAIAKTTTVIIIAHRLSTVVDADRIAVVEGGRIRAVGRHTDLLLIDDLYRELIEAQLL
                     AS"
     sig_peptide     complement(1598..1738)
                     /locus_tag="Tfu_1142"
                     /note="Signal predicted by SignalP 2.0 HMM (Signal peptide
                     probabilty 0.999) with cleavage site probability 0.907 at
                     residue 47"
     gene            complement(1746..2162)
                     /locus_tag="Tfu_1143"
     CDS             complement(1746..2162)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /locus_tag="Tfu_1143"
                     /product="conserved hypothetical protein"
                     /protein_id="AAZ55181.1"
                     /sec_met_domain="PF13471 (E-value: 4.9e-29, bitscore: 89.2,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSENVVLQRSNVRLSWRTKWAARCAVGAARLLARKPPERIRATLL
                     RLRGEVRPATYEEAKAARDAVLAVSLRCAGLRACLQRSLAIALLCRMRGTWATWCVGVP
                     RRPPFIGHAWVEAEGRLVEEGVGYDYFSRLITVD"
     gene            complement(2159..2431)
                     /locus_tag="Tfu_1144"
     CDS             complement(2159..2431)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Lasso_Fused_RRE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /locus_tag="Tfu_1144"
                     /product="conserved hypothetical protein"
                     /protein_id="AAZ55182.1"
                     /sec_met_domain="PF05402 (E-value: 2.4e-19, bitscore: 58.0,
                     seeds: 461, tool: rule-based-clusters)"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 6.1e-27,
                     bitscore: 82.1, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="Lasso_Fused_RRE (E-value: 4e-10, bitscore:
                     28.2, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METTGAEFRLRPEISVAQTDYGMVLLDGRSGEYWQLNDTAALIVQ
                     RLLDGHSPADVAQFLTSEYEVERTDAERDIAALVTSLKENGMALP"
     gene            complement(2412..4244)
                     /locus_tag="Tfu_1145"
     CDS             complement(2412..4244)
                     /EC_number="6.3.5.4"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177:asparagine synthase (glutamine-hydrolyzing)
                     (Score: 93.1; E-value: 2.7e-28)"
                     /gene_kind="biosynthetic"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /locus_tag="Tfu_1145"
                     /product="asparagine synthase (glutamine-hydrolysing)"
                     /protein_id="AAZ55183.1"
                     /sec_met_domain="Asn_synthase (E-value: 7.5e-22, bitscore:
                     66.8, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVGCISPYFAVFPDKDVLGQATDRLPAAQTLASHPSGRPWLVGAL
                     PADQLLLVEAGERRLAVIGHCSAEPERLRAELAQIDDVAQFDRIARTLDGSFHLVVVVG
                     DQMRIQGSVSGLRRVFHAHVGTARIAADRSDVLAAVLGVSPDPDVLALRMFNGLPYPLS
                     ELPPWPGVEHVPAWHYLSLGLHDGRHRVVQWWHPPEAELAVTAAAPLLRTALAGAVDTR
                     TRGGGVVSADLSGGLDSTPLCALAARGPAKVVALTFSSGLDTDDDLRWAKIAHQSFPSV
                     EHVVLSPEDIPGFYAGLDGEFPLLDEPSVAMLSTPRILSRLHTARAHGSRLHMDGLGGD
                     QLLTGSLSLYHDLLWQRPWTALPLIRGHRLLAGLSLSETFASLADRRDLRAWLADIRHS
                     IATGEPPRRSLFGWDVLPKCGPWLTAEARERVLARFDAVLESLEPLAPTRGRHADLAAI
                     RAAGRDLRLLHQLGSSDLPRMESPFLDDRVVEACLQVRHEGRMNPFEFKSLMKTAMASL
                     LPAEFLTRQSKTDGTPLAAEGFTEQRDRIIQIWRESRLAELGLIHPDVLVERVKQPYSF
                     RGPDWGMELTLTVELWLRSRERVLQGANGGDNRS"
ORIGIN
        1 ttcacgacgc gagaagctgg gcttcgatca gctcccggta gaggtcgtcg atgagaagca
       61 ggtcggtgtg tctgcccacg gcccgaatcc ggccgccttc cacaaccgcg atccggtccg
      121 cgtccaccac cgtcgacaac cggtgcgcga tgatgatgac cgtcgtcgtc ttcgcgatag
      181 cgacgaccac gtcccgcagc gcggtctcgt tggtcgcgtc caactgcgac gtcgcctcgt
      241 ccaacagcag cagccggggc cgccgcagca gggcccgggc gatcgccacc cgctggcgct
      301 caccccccga caaggtggtg ccccggtggc cgacctgcgt gtccaacccg tccggcagcc
      361 gctccacgag agaggtgagc cgggtgagtt ccacgacccg ccggatctct tcttcgtcgg
      421 cgtgtggggc cgcaaagcag aggttgtcgc gcagcgtgcc cgccaggatg ggggcgtcct
      481 gctccacgta gccgatcatg gaccgcagcc ggaccagcgg aatgtcgcgc acgtcgacgc
      541 cgtcgatggc gaccacgccc tcatgcggat cgtagaaccg ctccaccagg gaaaacagcg
      601 tagtcttccc tgccccggag gggcccacca gtgcggtcag cccgcccgcg ggcacgtcca
      661 gcgtcacatt ccgcaaggtc cacggcccgt cctcgcggta ccggaacgac accccggtga
      721 acgagaccga agccgcggag gtcgcggccg gggtgcgccg cggcggtacg ccgatgtgct
      781 ccactgggag ctgctccact tccgcgatgc ggcgcatcgc agccagcccg ctctgcaggc
      841 tgctgaccgc ctgcgccagt tgggcgacga aaccggtgag ccgcatcagg tagagcagga
      901 acgcgatcag ctcggagacg tcgatcgccc ccatcgctac ccgggcgccg cctgccccca
      961 gcaccacgag gaacgcggcc tgcgacgtga cgacaacagc gacgttggtc gccgcggtcc
     1021 acgccgcgga acgcacccct tcccgccacg cggtccgcac cgcggccttg gccgcgctga
     1081 tctcccgccg ctccgctgag ctggctttga gcgtgcggaa cgcgcccagt gcgcgctcca
     1141 ccagcgcgcc cacctcgccc acagcccgct gcgaacgttc gttggccctg cggatcggcg
     1201 gcatcatcac ggccgacccc accccgatca ccccgatcac ggccaggcac accagcagca
     1261 gcggcaggtc gatccaggcc atgagcacga tcgagccgat cgcgaccagc agcgcgctcc
     1321 cggagtcgac cagggcctgg ccggccgcgg aacggatgta ggtggtgtcg gacgtcaccc
     1381 gggagagcag gtcgccgggc ttgagccggt cgagctcggg cacgcggagc agcagaatgt
     1441 gggagaccag gcgtctccgg gccgcggcga ccatgctctc ggcggtgcgg tgcagcacat
     1501 aggagctcag cccggcaagc gcggccccgc cgacgaccag cacggtgagc agggccagca
     1561 gccagcccag cggggaccct tctcctagtg cgtccaccac ggccatggcc accagcggct
     1621 cagccagggt gccgataccg ccgagcagtc cgaggagcgc accgaaggcc acggcccggc
     1681 ggtgcggcca caggtgggcg acgagcaggc gcagggagcg gggaggagac agaggcacgg
     1741 cagggtcagt ccacggtgat cagacggctg aagtagtcgt agccgacgcc ttcttccacg
     1801 aggcgtcctt cggcctccac ccaggcgtgt ccgatgaacg gcgggcggcg gggaaccccc
     1861 acacaccagg tggcccaggt gccgcgcatc cggcacagca gtgcgatagc cagcgaacgc
     1921 tgcaggcagg cccgcagtcc ggcgcagcgc aggctgaccg cgagcaccgc gtcccgggcg
     1981 gctttggcct cttcgtaggt ggcgggccgc acctcgcccc ggagacgcag cagggtagcg
     2041 cggatccgct ccgggggttt gcgggccagg agccgggccg ctcccaccgc gcagcgcgcc
     2101 gcccacttcg tccgccacga caggcgcacg ttgctgcgct gcagcactac gttctcgctc
     2161 atggcagcgc catcccgttc tctttcaggc tggtgacgag cgcggcgatg tcgcgttccg
     2221 cgtcggtccg ctccacctca tattcgctgg tgaggaactg ggccacgtcg gcgggggagt
     2281 gcccgtccag cagccgctgc acgatcagcg ccgcggtgtc gttgagctgc cagtactcac
     2341 cgctgcggcc gtccagcagc accatgccgt agtcggtttg cgcgactgag atctcgggac
     2401 gtagccggaa ttcagctcct gttgtctcca ccgtttgctc cttggaggac acgttcgcgg
     2461 gaccgcagcc acagctcgac ggtgagggtg agctccatgc cccagtccgg gccccggaac
     2521 gagtagggct gtttgacccg ttccacgagc acgtcggggt ggatgagccc cagttccgcc
     2581 agtcgggact cccgccatat ctggatgatg cgatcgcgtt gttcggtgaa cccctccgca
     2641 gcgagcggag tgccgtcggt cttcgactgg cgggtcaaga actccgcggg gaggagggaa
     2701 gccatggccg ttttcatgag ggacttgaat tcgaacgggt tcatccggcc ttcgtggcgc
     2761 acctgaaggc aggcctccac cacgcggtcg tcgaggaacg gcgattccat gcggggcagg
     2821 tcgctgctgc cgagctggtg cagcagtcgc aggtcgcggc cggccgcgcg gatcgcggcc
     2881 aggtcagcgt gcctgcctcg ggtcggggcg agaggctcca gcgactccag gaccgcgtcg
     2941 aagcgggcca gcacgcgctc gcgcgcctcg gcggtcagcc acggcccgca cttgggcagg
     3001 acgtcccatc cgaacaggga gcggcgcggc ggttctcccg tggcgatgga gtgccggata
     3061 tcggcaagcc atgcgcggag gtcacggcgg tcagcgagcg aagcgaaggt ctctgacagc
     3121 gacagtcccg ccaggaggcg gtggccgcgg atgagcggca aggcagtcca cggccgctgc
     3181 cagagcagat cgtggtagag gctgagcgat ccggtgagga gctggtcgcc gcccaggccg
     3241 tccatgtgca ggcgggagcc gtgggcgcgg gccgtgtgca gccggctcag aatgcgcggc
     3301 gtgctcaaca tggccacgga cggttcgtcc aacaggggga actccccgtc gaggccagcg
     3361 tagaagccgg ggatgtcctc gggggagagc accacgtgct ccaccgaggg aaaggactgg
     3421 tgggcgatct tcgcccagcg gagatcatcg tccgtgtcaa ggccgctgct gaaggtcagg
     3481 gcgacgactt tggccgggcc gcgagcggcc agggcgcaga ggggagtgga gtcgagcccg
     3541 ccggacaggt cggcgctaac cactccgcca ccgcgggtgc gggtgtccac ggctccggcc
     3601 agggcagtgc gcagcagggg agctgcggcg gtgacggcga gttccgcttc cgggggatgc
     3661 caccactgga cgacccggtg gcggccgtca tgcaagccga ggctgaggta gtgccaggcg
     3721 gggacgtgct ccaccccggg ccatgggggc agctcgctga gcgggtaggg caggccgttg
     3781 aacatccgca gcgcgagcac gtccgggtcg ggggagacgc cgagtaccgc ggcgagcacg
     3841 tcggagcggt ccgcggcgat tcgggctgta ccgacgtggg cgtggaagac tcggcggagc
     3901 ccggacacgc ttccttggat ccgcatctgg tcgccgacga ccacgaccag gtgaaagctg
     3961 ccgtccaggg tgcgcgcgat gcggtcgaac tgggccacgt cgtcgatctg ggcgagttcc
     4021 gcacgcagcc gctcaggctc agccgaacag tgtccgatca cggcgaggcg gcgctccccg
     4081 gcctcgacca gcaggagctg gtcggcgggg agcgcgccta caagccaagg gcgtcccgaa
     4141 gggtgggacg ccagcgtctg ggctgccgga aggcggtcgg ttgcctgccc gagaacgtcc
     4201 ttatcaggga aaacagcgaa gtaaggactg atgcaaccga ccactggtcc tccggcgaga
     4261 atgacagata tttttgtgtg gaattcctac tatctatcag atgaagcgcg ggaagacgaa
     4321 gatcagctcg aggccccatt ccgcggtgta ccagccggtg gcctccttga attcgccgac
     4381 cttagcgagc tgcggagcgg tgtacttctt cttttccatc tctacctctc ctttggaata
     4441 gccgtatcgg agtgagcgct ctgtgtaact gccggcacgt ggcacaaagg ttaggggatc
     4501 tagtgacata tggcaacaga tgaggtcaga aaggattggt aagacgttta aaggagaaaa
     4561 agaccgcgtg gtctgtcgga taacgtcccg gtgaagacga tcgtttctgc tgctcaaaac
     4621 gggttttatt ggggtgctgt ccggtctgcg gttgcgtgtg gggcgcaggg gagaggtctg
     4681 atgagcgagg tgtttcgccc cgggatgcgt gcgggttctg ccctggagtg tgggatccgc
     4741 cgatccggcg ctgaccactg gtttcgtgca gttgagaaca aaaaagaacg ggcgctagag
     4801 agaggtgggg tgaaaaaaag attgccgccc ggctgccaga agaagacctt gtgcaggaat
     4861 agatccgaaa gtatttgaat attcaaagac gaggggaaga atttttcggg atttctctgg
     4921 tctagcgaaa aggttttcgc aggaagaaca ggggatgcgg gccgctgtgg ccgggggcac
     4981 accggttttt ctgttgaaga ggcgggccgg cggtggtatg tcaccctgtc tcccccgcag
     5041 gggatacgct caaggcggta cgacccggag aagacaggtg ggagccggtc
//
