LOCUS       BGC0001891             10865 bp    DNA     linear   BCT 25-AUG-2017
DEFINITION  Ruminiclostridium cellulolyticum H10 chromosome, complete genome.
ACCESSION   BGC0001891
VERSION     BGC0001891
KEYWORDS    .
SOURCE      Ruminiclostridium cellulolyticum H10
  ORGANISM  Ruminiclostridium cellulolyticum H10
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
            Ruminiclostridium.
COMMENT     URL -- http://www.jgi.doe.gov
            JGI Project ID: 4002584
            Source DNA and bacteria available from Jizhong Zhou
            (jzhou@rccc.ou.edu)
            Contacts: Jizhong Zhou (jzhou@rccc.ou.edu)
            David Bruce (microbe@cuba.jgi-psf.org)
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LANL
            Finished microbial genomes have been curated to close all gaps with
            greater than 98% coverage of at least two independent clones. Each
            base pair has a minimum q (quality) value of 30 and the total error
            rate is less than one per 50000.
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. it is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 16:30:50
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..10865
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[2150:10865]"
                     /cutoff="20000"
                     /detection_rule="(ATP-grasp and AMP-binding and PP-binding
                     and (DUF98 or Orn_Arg_deC_N or Orn_DAP_Arg_deC))"
                     /neighbourhood="20000"
                     /product="thioamide-NRP"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2151..10865
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(ATP-grasp and AMP-binding and PP-binding
                     and (DUF98 or Orn_Arg_deC_N or Orn_DAP_Arg_deC))"
                     /neighbourhood="20000"
                     /product="thioamide-NRP"
                     /protocluster_number="1"
     cand_cluster    1..10865
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(ATP-grasp and AMP-binding and PP-binding
                     and (DUF98 or Orn_Arg_deC_N or Orn_DAP_Arg_deC))"
                     /kind="single"
                     /product="thioamide-NRP"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..10865
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001891"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..10865
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="thioamide-NRP"
                     /region_number="1"
                     /rules="(ATP-grasp and AMP-binding and PP-binding and
                     (DUF98 or Orn_Arg_deC_N or Orn_DAP_Arg_deC))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(2..1096)
                     /locus_tag="Ccel_3250"
     CDS             complement(2..1096)
                     /codon_start=1
                     /db_xref="InterPro:IPR001395"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039:aldo/keto reductase family oxidoreductase (Score:
                     134.8; E-value: 5.9e-41)"
                     /gene_kind="biosynthetic-additional"
                     /inference="protein motif:PFAM:PF00248"
                     /locus_tag="Ccel_3250"
                     /note="PFAM: aldo/keto reductase; KEGG: amt:Amet_0907
                     aldo/keto reductase"
                     /product="aldo/keto reductase"
                     /protein_id="ACL77539.1"
                     /transl_table=11
                     /translation="MLYKRYGNSDVELSAIGMGCMRFDDHVILENKFEKYVELILYAHE
                     NGINYFDTAPFYCHDKSEIITGMALSQLPRSSYYVASKTNLATLQNECTVDGFRRRLET
                     SLKRLKVDYLDFYYLWCMLDLNSFYENCEKLFGFFQQAKSEGLIRRIVMSSHMQGDDLV
                     RALDTKLFEGVALGYNALNYRYRKSGIEAAYNSGMGISVMNPLGGGVIVDNPRVFKFLT
                     EGTNLNVAQGGLRFVAAQKEVSVTMTGFTTKKHVNDAVKAIENLEIKPMAEICKEYENK
                     GIILNDLCTGCGYCNHCPQNIEIPKFMDAYNDKLLGRSIVKRLRRHWHLSAKAAVGCIK
                     CGKCEKMCTQHLPIIERIQEIASL"
     gene            complement(1190..2125)
                     /locus_tag="Ccel_3251"
     CDS             complement(1190..2125)
                     /codon_start=1
                     /inference="similar to AA sequence:KEGG:FTH_0345"
                     /locus_tag="Ccel_3251"
                     /note="KEGG: fth:FTH_0345 hypothetical protein"
                     /product="conserved hypothetical protein"
                     /protein_id="ACL77540.1"
                     /transl_table=11
                     /translation="MNIDKAVLDHYLSTSTYTYAGVYKDYFKSLPDDLKELSELVGQQY
                     IQRFVLKCGNSGENENLRYGDMDRFPWYRMCCEDDILLTAVAMTAEIFRLNEKGFFPGR
                     QVEERIVISCRHITVILGAILKAKGIPCRSRAGIRAYHGKPVDHWINQYWSEKENRWVN
                     FDGEGIMYRECEGLFTTYDIPDDQFYWIAQLWLDVRSGKLDGNNYILLDGSKSLKALAK
                     IMMYDFHALMNDEVNYHFIPYFIDNNRLEIDEGIAKEIDGLAELMVNPDENFKELVNVW
                     ETNRKFRVLNAPLIRTMDNSKVLFESTSNG"
     gene            complement(2151..3575)
                     /locus_tag="Ccel_3252"
     CDS             complement(2151..3575)
                     /NRPS_PKS="Domain: AMP-binding (12-387). E-value: 4.4e-71.
                     Score: 231.4. Matches aSDomain:
                     nrpspksdomains_Ccel_3252_AMP-binding.1"
                     /codon_start=1
                     /db_xref="InterPro:IPR000873"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thioamide-NRP: AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     261.2; E-value: 2.5e-79)"
                     /gene_kind="biosynthetic"
                     /inference="protein motif:PFAM:PF00501"
                     /locus_tag="Ccel_3252"
                     /note="PFAM: AMP-dependent synthetase and ligase; KEGG:
                     hmo:HM1_0197 long-chain-fatty-acid-CoA ligase"
                     /product="AMP-dependent synthetase and ligase"
                     /protein_id="ACL77541.1"
                     /sec_met_domain="AMP-binding (E-value: 2e-75, bitscore:
                     243.3, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDCFYSVLDSQSTQVFYIDGENKYSYAQLWNLISRARVDFKEKG
                     IKINQRVPILCNDPLHFIVGLLSIISLGACAVLIETSKKPKEIIEILEQIDSKIILTDQ
                     YMGSDLSSYDVTITEFSLKNLTEEKDSRISCEMNSEGCIIYTSGSTSKPKGVIRSNRIL
                     FEHSKMLQRTYNLSSNDTFLCLVQPQHAFGLENVLGAIYSGATLVVQKNFSHTEVINFI
                     ENGKCSVVVGVPFQYELLTKVNKKVPVNKLRYFLSAGAPLKKDVNAAIYSLFGIPVTQI
                     YGSSELGATAINIDISKSFEYDAVGKPLPEVQIKIIDSENRVLENNQIGEIVLRSPYCT
                     IGYVGVSEVSKTDAYIEDGWFYSGDLGYINDKGVLFITGRKKNVINIAGKKVSPEEVER
                     VIKGIEKILDVKVEGEKNSLSGETITAKVVVENGADISEQLVLNRCKELLSDYKIPRHI
                     VFTDKLEYTSTGKLKR"
     CDS_motif       complement(2286..2318)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Ccel_3252_0003"
                     /evalue="9.90E+01"
                     /label="NRPS-A_a3"
                     /locus_tag="Ccel_3252"
                     /protein_end="430"
                     /protein_start="419"
                     /score="-3.1"
                     /tool="antismash"
                     /translation="SGETITAKVVV"
     CDS_motif       complement(2382..2444)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Ccel_3252_0002"
                     /evalue="1.30E-03"
                     /label="NRPS-A_a8"
                     /locus_tag="Ccel_3252"
                     /protein_end="398"
                     /protein_start="377"
                     /score="11.6"
                     /tool="antismash"
                     /translation="TGRKKNVINIAGKKVSPEEVE"
     aSDomain        complement(2415..3539)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Ccel_3252_AMP-binding.1"
                     /evalue="4.40E-71"
                     /label="Ccel_3252_AMP-binding.1"
                     /locus_tag="Ccel_3252"
                     /protein_end="387"
                     /protein_start="12"
                     /score="231.4"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="STQVFYIDGENKYSYAQLWNLISRARVDFKEKGIKINQRVPILCN
                     DPLHFIVGLLSIISLGACAVLIETSKKPKEIIEILEQIDSKIILTDQYMGSDLSSYDVT
                     ITEFSLKNLTEEKDSRISCEMNSEGCIIYTSGSTSKPKGVIRSNRILFEHSKMLQRTYN
                     LSSNDTFLCLVQPQHAFGLENVLGAIYSGATLVVQKNFSHTEVINFIENGKCSVVVGVP
                     FQYELLTKVNKKVPVNKLRYFLSAGAPLKKDVNAAIYSLFGIPVTQIYGSSELGATAIN
                     IDISKSFEYDAVGKPLPEVQIKIIDSENRVLENNQIGEIVLRSPYCTIGYVGVSEVSKT
                     DAYIEDGWFYSGDLGYINDKGVLFITGRKKNVINI"
     CDS_motif       complement(3105..3152)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Ccel_3252_0001"
                     /evalue="4.60E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="Ccel_3252"
                     /protein_end="157"
                     /protein_start="141"
                     /score="22.3"
                     /tool="antismash"
                     /translation="CIIYTSGSTSKPKGVI"
     gene            complement(3607..3885)
                     /locus_tag="Ccel_3253"
     CDS             complement(3607..3885)
                     /NRPS_PKS="Domain: PP-binding (6-78). E-value: 4.5e-17.
                     Score: 54.3. Matches aSDomain:
                     nrpspksdomains_Ccel_3253_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thioamide-NRP: PP-binding"
                     /gene_kind="biosynthetic"
                     /inference="ab initio prediction:Prodigal:1.4"
                     /locus_tag="Ccel_3253"
                     /note="KEGG: acyl carrier protein, mitochondrial precursor,
                     putative; K03955 NADH dehydrogenase (ubiquinone) 1
                     alpha/beta subcomplex 1"
                     /product="hypothetical protein"
                     /protein_id="ACL77542.1"
                     /sec_met_domain="PP-binding (E-value: 8.9e-18, bitscore:
                     54.3, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNYSDYDKFLNILREMLYENLKDMEITLDSDLKEDLFLDSISFYT
                     LIVSLEQAFNFTFSKVNIDADEFKTVRSMIAYMDLLRTSNQYNELEV"
     aSDomain        complement(3652..3867)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Ccel_3253_PP-binding.1"
                     /evalue="4.50E-17"
                     /label="Ccel_3253_PP-binding.1"
                     /locus_tag="Ccel_3253"
                     /protein_end="78"
                     /protein_start="6"
                     /score="54.3"
                     /tool="antismash"
                     /translation="DKFLNILREMLYENLKDMEITLDSDLKEDLFLDSISFYTLIVSLE
                     QAFNFTFSKVNIDADEFKTVRSMIAYM"
     gene            complement(3964..4893)
                     /locus_tag="Ccel_3254"
     CDS             complement(3964..4893)
                     /codon_start=1
                     /inference="ab initio prediction:Prodigal:1.4"
                     /locus_tag="Ccel_3254"
                     /product="hypothetical protein"
                     /protein_id="ACL77543.1"
                     /transl_table=11
                     /translation="MLLDTFQGYNCYSSALGEYAKQKNIDQVENIILSQWSFFFDEEQF
                     YKNQWYTGAADGPVDVVLNEDLRNFANIEVLEHISSESQAIDEGRKVLEKHGLQIVLMD
                     FYYMNSFNWKSLSRFNVTREHDPHFAVLTQINENSVHIIDPYYHHEENMSMEDFIKSRN
                     SMTKQGKISFNSYEIFSNGTKKSNIKELLYYRFNRYLQEKMFGKITQFGQVVKKQLDNK
                     DRKWAFTGYNCLNSVVYQHQNLINLQKKFSLEMPPNLQELLDNWALIRKKLFEYYSRGS
                     YNTEEISNLICKVASSEEQFAQEVLKVL"
     gene            complement(4942..6189)
                     /locus_tag="Ccel_3255"
     CDS             complement(4942..6189)
                     /codon_start=1
                     /db_xref="InterPro:IPR000183"
                     /db_xref="InterPro:IPR002986"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thioamide-NRP: Orn_DAP_Arg_deC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thioamide-NRP: Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264:diaminopimelate decarboxylase (Score: 334.5;
                     E-value: 1e-101)"
                     /gene_kind="biosynthetic"
                     /inference="protein motif:PFAM:PF00278"
                     /locus_tag="Ccel_3255"
                     /note="PFAM: Orn/DAP/Arg decarboxylase 2; KEGG:
                     gme:Gmet_2019 Orn/DAP/Arg decarboxylase 2"
                     /product="Orn/DAP/Arg decarboxylase 2"
                     /protein_id="ACL77544.1"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 6.1e-46, bitscore:
                     146.3, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 5.6e-20,
                     bitscore: 61.1, seeds: 500, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDDRQIINIAHKYKTPLYLFDGDRLEETYFNMKKVLPDAFEFFYS
                     VKANPSYGICRILQKCSSGIEVASAGELHLALEAGFDNKKILFTGPGKTYSELEFAVDS
                     KILAINAESYKEIMLIDDIAKAKNKIVDVGLRIHPNFKIESKNPAISMMGTGTQFGVDI
                     DEIPEILKYIKSSKNLNLVCFHVYAGSQIFQPDTTLAYFEQTINIFKELIEKNDLHIKV
                     LDFGGGFGVSYDGKNKPYDFEYFGAEINKLYHKYEDFFQGKKLVFESGRLLLAESGVFL
                     TEVQYRKVLHDKVFLITDGGMNHNSLSTFREKKIRGNFLMKILDNRNEEETVTVAGPLC
                     TPEDILGRNVTLNKADRGDILCIKNTGAYGSSFSPLGFLGHPAPCEVLIYKNNEYYLKK
                     HGVFKNILNGQEIIEL"
     gene            complement(6217..6468)
                     /locus_tag="Ccel_3256"
     CDS             complement(6217..6468)
                     /NRPS_PKS="Domain: PP-binding (2-71). E-value: 1.6e-12.
                     Score: 39.7. Matches aSDomain:
                     nrpspksdomains_Ccel_3256_PP-binding.1"
                     /codon_start=1
                     /db_xref="InterPro:IPR006163"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thioamide-NRP: PP-binding"
                     /gene_kind="biosynthetic"
                     /inference="protein motif:PFAM:PF00550"
                     /locus_tag="Ccel_3256"
                     /note="PFAM: phosphopantetheine-binding; KEGG:
                     sat:SYN_01151 acyl carrier protein"
                     /product="phosphopantetheine-binding"
                     /protein_id="ACL77545.1"
                     /sec_met_domain="PP-binding (E-value: 3.1e-13, bitscore:
                     39.7, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEKIKEIICSHTYIKREDLDSIGDNDKLTELGLDSINVVYIIGE
                     IEEEFNFSFYDEDMLLVNFETIDKILKTVGKYVSVKSV"
     aSDomain        complement(6256..6462)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Ccel_3256_PP-binding.1"
                     /evalue="1.60E-12"
                     /label="Ccel_3256_PP-binding.1"
                     /locus_tag="Ccel_3256"
                     /protein_end="71"
                     /protein_start="2"
                     /score="39.7"
                     /tool="antismash"
                     /translation="EKIKEIICSHTYIKREDLDSIGDNDKLTELGLDSINVVYIIGEIE
                     EEFNFSFYDEDMLLVNFETIDKIL"
     gene            complement(6498..7811)
                     /locus_tag="Ccel_3257"
     CDS             complement(6498..7811)
                     /codon_start=1
                     /db_xref="InterPro:IPR011761"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thioamide-NRP: ATP-grasp"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_kind="biosynthetic"
                     /inference="ab initio prediction:Prodigal:1.4"
                     /locus_tag="Ccel_3257"
                     /note="KEGG: saq:Sare_0485 hypothetical protein"
                     /product="hypothetical protein"
                     /protein_id="ACL77546.1"
                     /sec_met_domain="ATP-grasp (E-value: 5.6e-13, bitscore:
                     38.4, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 3.3e-10, bitscore:
                     29.8, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIIKDSGNQKILIYNSGIEHEWEDKKTGVRKVNNLKEQKILNRQ
                     AELLFFISNAEDTVYVVKESDPEFLKDMEVFGISKPKTVVIPDLDLPISKIIVENEDIL
                     QDLKTRFAGQKVLYVPYILSSHDEKISEYCNFAIYGSSSDLITKLNNKANARRIVEEIG
                     LSCIEGGICSSKEELEEQYNKLKGKGYMRFVLKEPYNSAGKGVFFIKDEKQFYSFLKMM
                     RFNNENKNFEVIIEGWIDDKRDINYQIEISKEGEVKLIAITEQIISVTAYKGSLYPPKL
                     TKQQEDYYNDCAQKLGTKLYQMGFSGIIGIDSIIDKDGTIFPAIEFNARFNQSTFYIPF
                     LNYFRNHSKRILIRSYDVKTNNSLNYVRLKEILRENKLLYCNESKKGIIILNSSCLSID
                     KDSDGQYDNRIYLANVYDKNDKDDSRYEEMDNLIKVIN"
     gene            complement(7808..8875)
                     /locus_tag="Ccel_3258"
     CDS             complement(7808..8875)
                     /codon_start=1
                     /inference="similar to AA sequence:KEGG:Dde_1603"
                     /locus_tag="Ccel_3258"
                     /note="KEGG: dde:Dde_1603 hypothetical protein"
                     /product="conserved hypothetical protein"
                     /protein_id="ACL77547.1"
                     /transl_table=11
                     /translation="MIECEVKCKKCLKQIPVDSEGKSMAKNGLCLECYNDELKAQSIDW
                     MEKEQELQEKIEEVRRAKGTFDGLVMLSGGKDSIYVAYILSKVYKLNIVGLTIDNGFEY
                     QSTFDNSYDIAKKLGISHFIYRLPFEDMREYYNFLLTEDTLKLQDCSQLCFFCGRLLKT
                     ISIKVAKMLNVTAVFSGHTLEQIRALGDEEGKDKSFDIRRRYIQNYSVQNYKKALDLLK
                     EKSKESISYLFDDNLETLNFDRFIYPLQYFEYKPIEIVELLSKELGWVADTHFTKKYIS
                     SGCKLGKLMEYIATKNGSTTYVEREFSDQIRRGSLSKEDVIKIIESRVDDEGEIQEIID
                     LLSTSKEKLFWVGEK"
     gene            complement(8939..10249)
                     /locus_tag="Ccel_3259"
     CDS             complement(8939..10249)
                     /NRPS_PKS="Domain: Aminotran_3 (19-367). E-value: 4.6e-87.
                     Score: 284.0. Matches aSDomain:
                     nrpspksdomains_Ccel_3259_Aminotran_3.1"
                     /codon_start=1
                     /db_xref="InterPro:IPR005814"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013:aminotransferase class-III (Score: 324.5;
                     E-value: 1.7e-98)"
                     /gene_kind="biosynthetic-additional"
                     /inference="protein motif:PFAM:PF00202"
                     /locus_tag="Ccel_3259"
                     /note="PFAM: aminotransferase class-III; KEGG: bca:BCE_4189
                     adenosylmethionine--8-amino-7-oxononanoate transaminase"
                     /product="aminotransferase class-III"
                     /protein_id="ACL77548.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.3e-88, bitscore:
                     285.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGSTYWRPFNRADGENVINIVKGEGIFLYDDSGRRFQDAYSGLWN
                     MNYGYSDNDIKKAIKTQIEELPYINPITLGNPKASELADKLCSITHDEITKVLFTCSGS
                     EAIEAAIKISRKYNNLIGKKQYHIAVISESYHGSYYGSMSASFYDENEKQGYGPMVDGF
                     IKLVLPFSRRCKTEEMTIDEKNKVMQKLEETLQNNKDKLCAIIVEPILASGGVIPLFEE
                     YLSRINSFCRENNVLFVCDEVATGFGRTGTMFRFQKFDLKPDIITMSKGINNGYLPLGA
                     VCISEKIESAFLKENQILFHLSTQNANPICLAAALATIDKMERDNILEVVNAKSTYFKK
                     ILNDDLSNLSMVFEIRIHGLMAAIDLADRETNNPISHEQLIKVISTIYSLGCFAGISYT
                     KDITSSILIFPQYIATESDLDNIVKIVKTAISNSSGI"
     aSDomain        complement(9149..10192)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Ccel_3259_Aminotran_3.1"
                     /evalue="4.60E-87"
                     /label="Ccel_3259_Aminotran_3.1"
                     /locus_tag="Ccel_3259"
                     /protein_end="367"
                     /protein_start="19"
                     /score="284.0"
                     /tool="antismash"
                     /translation="IVKGEGIFLYDDSGRRFQDAYSGLWNMNYGYSDNDIKKAIKTQIE
                     ELPYINPITLGNPKASELADKLCSITHDEITKVLFTCSGSEAIEAAIKISRKYNNLIGK
                     KQYHIAVISESYHGSYYGSMSASFYDENEKQGYGPMVDGFIKLVLPFSRRCKTEEMTID
                     EKNKVMQKLEETLQNNKDKLCAIIVEPILASGGVIPLFEEYLSRINSFCRENNVLFVCD
                     EVATGFGRTGTMFRFQKFDLKPDIITMSKGINNGYLPLGAVCISEKIESAFLKENQILF
                     HLSTQNANPICLAAALATIDKMERDNILEVVNAKSTYFKKILNDDLSNLSMVFEIRIHG
                     LMAAIDLA"
     gene            complement(10296..10865)
                     /locus_tag="Ccel_3260"
     CDS             complement(10296..10865)
                     /codon_start=1
                     /db_xref="InterPro:IPR002800"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thioamide-NRP: DUF98"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1259:hypothetical protein (Score: 62.3; E-value:
                     7.8e-19)"
                     /gene_kind="biosynthetic"
                     /inference="protein motif:PFAM:PF01947"
                     /locus_tag="Ccel_3260"
                     /note="PFAM: protein of unknown function DUF98; KEGG:
                     btl:BALH_2115 hypothetical protein"
                     /product="protein of unknown function DUF98"
                     /protein_id="ACL77549.1"
                     /sec_met_domain="DUF98 (E-value: 2.3e-13, bitscore: 39.9,
                     seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMQMNNSTNRMLLTEITQTERKLADILLVSKSSTTRLLEIMTGRE
                     VEINVTSQKVTEEKDLSENYYDYISPLKKSEKYLKRTVSLHSHGNIFSDNIVIASFDNI
                     SDEIKNALLRSKTPLGKIIKDNETKREILWSGYLNKNELNAIFGEQRFSLLEYPFKKYL
                     IYVNDNCCFCLLEVFNIKEIAKFFWN"
ORIGIN
        1 tttacaaaga agcaatttct tgtattctct ctattattgg caagtgttgt gtacacattt
       61 tttcacactt gccgcatttt atacatccta ctgctgcttt ggctgataga tgccaatgcc
      121 ttcgtaaacg ttttacgata gaacgaccaa gaagtttatc attgtatgca tccataaatt
      181 tgggaatctc aatattttgt ggacaatgat tacagtatcc gcatcctgta caaagatcgt
      241 tcaagattat acccttgttt tcatattctt tacatatctc tgccattggt tttatttcaa
      301 gattttcaat agcttttact gcatcattaa catgcttttt tgtagtaaat cccgtcattg
      361 ttaccgatac ttctttctga gcagcaacaa aacgcagtcc tccttgtgca acatttaagt
      421 tcgtaccttc cgtcagaaat ttaaaaaccc tcggattatc aacgataact ccgcctccca
      481 acggattcat aaccgaaatg cccataccgc tattataggc tgcttcaatg ccgctttttc
      541 tgtaacggta gtttaacgca ttgtagccga gtgctactcc ttcgaagagt ttagtatcca
      601 atgctcgtac caagtcgtca ccctgcatat gcgaagacat aactattctc cttataagtc
      661 cttctgactt agcttgttgg aaaaaaccaa acagcttctc acagttttca taaaatgagt
      721 ttaaatccaa catacaccaa agatagtaaa aatctaaata atctactttc aaacgtttta
      781 acgatgtttc aagacgtcta cgaaatccgt ctaccgtaca ttcgttttgc aatgtggcta
      841 aatttgtttt tgaagctaca tagtagctgc tacgagggag ttgagaaagt gccatgcccg
      901 ttataatttc acttttgtcg tgacagtaga atggtgccgt atcgaaatag ttaattccgt
      961 tctcatgagc atataaaatt aattccacgt atttttcaaa cttgttttct aagataacat
     1021 gatcatcaaa acgcatacag cccattccaa tagcagacaa ttctacatcg gagtttccat
     1081 agcgcttata aagcatttac aatcctccaa catttcaata aacatatatt aataacattg
     1141 ttcaatatac atttagtatt tttaaataac ttccctagac atatcctatt tatccgtttg
     1201 atgtagattc aaatagcact ttactgttat ccatagttct aataagcgga gcattcaaaa
     1261 cacggaattt tctgttagtt tcccaaacgt taaccaattc tttaaagttt tcatccgggt
     1321 taaccatcaa ttcagccaat ccatcaattt ctttggctat accttcgtct atttctaatc
     1381 tattattatc aataaagtat ggtatgaaat ggtaatttac ttcatcattc atcaatgcat
     1441 ggaaatcgta catcattatt tttgccaatg cctttagcga tttagatcca tccagaagta
     1501 tatagttatt accgtccagt ttaccgcttc taacatctaa ccaaagttgt gcaatccaat
     1561 aaaactgatc atcgggaata tcataagttg taaacaaccc ctcgcactca cgatacatta
     1621 ttccttcccc atcaaaatta acccatctgt tttctttttc actccagtat tggttaatcc
     1681 aatgatctac aggcttacca tgatatgccc ttattcctgc cctgctacgg caaggaatac
     1741 cttttgcttt taaaattgcc cctaatataa cagttatgtg tctacaggat attacaatcc
     1801 tctcctcaac ttgtctgccc gggaaaaaac ctttttcgtt taatcgaaat atttcagctg
     1861 tcatagcaac agcggttaac aatatatcgt cttcacaaca cattctgtac cagggaaacc
     1921 tatccatatc tccgtatcgc aaattttcat tttccccact gtttccgcat ttcagtacaa
     1981 acctttgtat atactgttga ccaaccagtt cactcaactc tttcaaatca tccggcaagc
     2041 ttttaaagta gtctttgtag acccctgcat atgtatatgt acttgttgac aggtaatgat
     2101 ccaaaacggc tttatcaata ttcatcaatt aaccctccat tttaaattaa ctatcttttt
     2161 aattttccag tgcttgtata ttcaagtttg tctgtaaaga caatatgtct gggaattttg
     2221 taatcggata aaagttcttt gcagcggttg agcaccaact gctcggatat atcagcacca
     2281 ttttctacaa cgacctttgc tgttatagtt tcaccgctta acgaattttt ttctccttct
     2341 acctttacat ccaaaatttt ttcaatccct tttattactc tttctacttc ttccggagat
     2401 actttctttc cggctatatt aattacattt ttctttctgc cagttataaa aagaacacct
     2461 ttatcattga tatagcctaa atctcccgag taaaaccacc cgtcttcaat ataagcatct
     2521 gtttttgaaa cctcactaac gccgacataa ccaatagtgc aataaggact tcttaaaaca
     2581 atctctccta tctgattatt ttcaagtact ctgttttcgc tatcaattat tttaatttgg
     2641 acctctggca aaggctttcc aactgcatca tattcaaaag atttggatat atcaatattt
     2701 atagcagttg cacctagctc tgaagaacca tagatttgcg ttaccggaat accaaagaga
     2761 ctatatattg cagcatttac atcttttttt aatggtgcac ctgcggaaag gaagtagcgt
     2821 agtttattta ccggtacctt tttatttact ttggttaaaa gctcatactg aaatggaaca
     2881 cctaccacta ctgaacattt tccattttca ataaaattaa taacttccgt atgagaaaag
     2941 tttttttgta ctaccagagt tgcaccacta taaattgctc ccaaaacatt ttctagacca
     3001 aatgcgtgct gtggttggac taaacacaga aaagtatcat tactacttaa attatatgtt
     3061 cgttgaagca tttttgaatg ctcaaacaat attcggttac tccgtataac ccctttgggt
     3121 ttggatgtac ttcctgaagt atatataata cacccctcgg aattcatttc gcagcttatc
     3181 ctactatcct tttcttctgt caagttcttt aaactaaatt ctgtaattgt aacatcatag
     3241 ctgcttaaat cactgcccat atactggtct gtaagaataa ttttactatc tatctgctct
     3301 aatatttcaa tgatttcctt tggcttttta ctggtttcaa ttaacactgc acatgcaccc
     3361 aatgatatta ttgatagcaa gccaacaata aagtgaagag ggtcattaca taaaattggt
     3421 actctttgat ttatcttaat ccctttctct ttaaaatcaa ctcgagctct ggatattaaa
     3481 ttccatagct gggcatatga gtatttattt tctccgtcaa tataaaaaac ctgggtactt
     3541 tggctatcca atactgaata gaaacagtcg ctcatattaa atatgttctc ctttctaaat
     3601 gtatggttat acttccagct cattatattg atttgaagtt cttaataagt ccatatatgc
     3661 aatcatggac cgtaccgttt taaactcgtc agcatcaata ttgactttac tgaatgtaaa
     3721 attgaaggcc tgttccaaac ttacaattaa ggtatagaag gagattgaat ctaaaaataa
     3781 atcctctttt aaatcgctat ccagtgtaat ctccatatcc ttcagatttt cgtaaagcat
     3841 ttctcttaat atgttaagaa atttatcata gtcagaataa ttcaatttat aacctcctct
     3901 tgcactttga taaaacctgt ccagtattag gcataacgac acttatttga ctatatgtta
     3961 ttcttataat accttcaaaa cctcttgtgc aaactgctcc tcagaagaag ctactttaca
     4021 tattaaattc gatatttctt cggtattgta actacctctg ctataatact caaatagctt
     4081 tttacgtata agtgcccaat tatccagtaa ttcttgcaga ttcggcggca tctctaaact
     4141 gaactttttt tgcaggttta ttaaattttg gtgttgataa actacagaat tcaaacaatt
     4201 gtaccctgtg aatgcccatt tacgatcttt gttatccaat tgttttttca caacctgacc
     4261 gaattgtgta atcttaccga acattttttc ttgtaaatac ctattaaaac gatagtataa
     4321 caattcttta atattacttt tttttgtacc gttactgaat atctcgtacg aattaaagct
     4381 aatcttacct tgtttcgtca tagagtttct tgatttaata aaatcctcca ttgacatatt
     4441 ttcttcatga tgatagtacg gatcaattat atgaacgctg ttttcgttta tttgtgtaag
     4501 aacagcaaaa tgcggatcgt gttctctggt tacattaaac cgacttaatg atttccaatt
     4561 aaacgaattc atatagtaaa aatccattaa aacaatctga aggccatgct tctctaaaac
     4621 tttcctgcct tcatcaatag cctgactctc tgaggatata tgttctaata cctcaatgtt
     4681 ggcaaaattt cttaaatcct catttaaaac cacatcaacc gggccatcag cggcacctgt
     4741 ataccattga tttttgtaaa attgctcttc atcgaaaaaa aatgaccatt gagacaaaat
     4801 tatattttcg acttggtcaa tatttttttg ctttgcatac tctcccaatg ctgacgaata
     4861 acaattatat ccttgaaatg tatccagtaa cattcttatc acctgacctc agacttttat
     4921 cacaattgta atctgctttc tttataattc aattatctct tgaccattta gaatattttt
     4981 aaatacgcca tgctttttta aataatactc attgtttttg taaataagta cttcacaggg
     5041 tgcaggatgc cccaaaaagc ctaacggact aaatgagctt ccatatgccc ctgtgttttt
     5101 aatacagaga atatcccctc tatccgcctt atttaaggtt acatttctac ctagtatgtc
     5161 ttcaggggta caaagtgggc cagccacagt taccgtctct tcttcatttc tgttatccaa
     5221 tattttcatt aagaaattgc ctcgaatttt cttttctcgg aaggttgaaa gtgaattatg
     5281 gttcataccg ccgtcagtta tcaagaatac cttgtcatga agtacttttc tgtattgtac
     5341 ctcggttaaa aataccccgc tttcggcaag taataacctt cctgactcga aaacaagttt
     5401 cttgccctga aaaaagtctt catatttgtg atacaattta tttatttctg ctccaaaata
     5461 ctcaaaatcg taaggtttat tcttcccatc gtaagaaacc ccaaatccac ctccgaaatc
     5521 cagtactttg atatgtaaat catttttttc aattaattct ttaaatatgt ttattgtctg
     5581 ttcaaaatag gcaagtgtcg tatccggctg aaatatctgg gaccctgcat atacatgaaa
     5641 gcatacaaga ttgagatttt ttgaggactt aatatatttg agtatttcgg gaatctcatc
     5701 aatatctaca ccaaattgag ttcctgtacc catcatggag attgcagggt ttttagattc
     5761 aattttaaag tttggatgta tccgtaaacc tacatcaaca atcttgtttt tcgctttagc
     5821 aatatcatca attaacataa tctccttgta ggattctgca ttgatggcca gtattttact
     5881 atctactgca aattctagtt cactgtacgt tttacctggc ccggtaaaaa gaattttttt
     5941 gttatcgaat ccggcctcca gagccaaatg aagttcaccc gctgaagcta cttctatccc
     6001 actagaacac ttctgtaaga ttctacatat gccatacgaa ggattagctt ttaccgaata
     6061 gaaaaattca aaggcatccg gcaacacttt tttcatgttg aaataagtct cttccagcct
     6121 atctccatca aaaaggtaca gtggagtttt atatttatgt gcaatattaa ttatttgcct
     6181 atcatccaca tttatcaacc cttcctgtaa aattctctaa acagatttta ccgatacata
     6241 tttcccaact gtttttagta ttttgtctat tgtttcaaaa ttaactaata acatatcctc
     6301 gtcataaaac gagaaattaa attcttcttc aatctctcca ataatataaa ctacatttat
     6361 ggaatctaat cccaattctg taagtttgtc attgtctcct attgaatcca gatcttccct
     6421 tttaatgtaa gtatgagaac atataatctc cttgattttt tctttcatag taaatacctc
     6481 ctataaaata tttgtattta attgattact ttaatcaaat tatccatttc ttcataacga
     6541 ctatcatctt tgtcgttttt atcataaaca tttgccagat atattctatt atcatattgt
     6601 ccgtctgaat ctttatctat ggacagacaa ctagaattta aaattataat tcctttttta
     6661 ctttcattgc aataaagcaa tttgttctca cgtaaaatct cttttaacct aacataattc
     6721 agactgttat tggttttaac atcatagctt ctgataagaa ttctcttact atgatttcta
     6781 aaatagttaa gaaaaggaat atagaatgtt gattggttga accgtgcatt gaactctata
     6841 gcgggaaata ttgtcccatc cttatcaata attgagtcta tgccaataat tccactaaac
     6901 cccatctggt ataatttagt tcctaatttt tgggcacagt cattgtagta atcctcttgc
     6961 tgctttgtta atttcggtgg gtataaagaa cccttgtatg ctgttaccga aatgatttgc
     7021 tctgtaattg caattaactt gacctctccc tcttttgata tttcgatttg ataattgata
     7081 tcccgtttat catcaatcca gccttcgatt attacttcga aattcttatt ctcattatta
     7141 aatctcatca ttttgaggaa actatagaat tgtttttcat cctttataaa gaaaacacct
     7201 tttccggcag aattataggg ttcctttagt acaaatctca tgtacccttt gccctttagt
     7261 ttattatact gctcctccaa ttcctctttg gaagaacata ttccaccctc aatacaggat
     7321 aaacctattt cttctactat tcttcgagca ttggctttat tattcaattt tgtaatcagg
     7381 tctgacgaag acccataaat agcaaagtta caatactcgc ttattttttc atcatggcta
     7441 ctcaatatat atggaacata taataccttt tgtcctgcaa atctcgtttt gagatcttgc
     7501 aagatatctt cgttctcaac aataatttta gaaattggta aatccaaatc aggaatcacg
     7561 acggttttgg gcttacttat accaaaaact tccatatctt ttaaaaactc aggatctgat
     7621 tctttcacaa cgtaaacggt atcttcggca ttagagataa aaaaaagtaa ttctgcctgc
     7681 ctattcagga ttttttgttc tttaagatta ttaacttttc ttacgccagt cttcttgtct
     7741 tcccattcat gctcgattcc tgaattataa attaatattt tttggttacc tgaatcttta
     7801 attatactca tttttcacct acccaaaata gcttctcttt ggatgtagat agtaaatcga
     7861 ttatttcctg tatctctcct tcatcgtcta ctctactttc aataattttt ataacatcct
     7921 cttttgataa tgagccgcgt cggatttgat cactgaattc tctttctaca tatgtagttg
     7981 aaccgttttt agtggcgata tattccataa gtttgccgag tttacatcct gacgagatat
     8041 atttcttggt aaaatgggta tccgctaccc atcctaattc cttactaagt aactcgacaa
     8101 tttcgatagg cttgtactca aagtattgca agggataaat gaacctgtca aaatttaaag
     8161 tctcaagatt atcatcaaat agataagaga tagactcctt gctcttttcc tttaaaagat
     8221 ctaatgcctt tttataattt tgaactgaat aattctgtat gtaacgccta cgaatatcga
     8281 aacttttatc tttaccttct tcatctccca aagcccttat ctgttccagt gtatggccgg
     8341 aaaatacagc cgtaacattc agcatctttg ctacttttat agaaattgtt ttcaggagtc
     8401 tcccgcagaa gaaacataat tggctgcaat cctgcaactt taaagtatct tcagtcagta
     8461 agaaattata gtactccctc atatcttcaa aaggcaggcg ataaatgaaa tgtgatattc
     8521 ccagtttctt agctatatcg tatgaattgt caaatgtact ttgatattcg aatccattat
     8581 cgattgttaa tccgacaata tttagcttgt agacttttga tagtatgtaa gcaacatata
     8641 tactgtcttt tcccccgctt aacattacca atccgtcaaa agttcctttt gctcttcgga
     8701 cctcttcgat tttttcttgg agttcctgct ccttttccat ccagtcaatg gattgagctt
     8761 ttaattcatc attgtaacat tccaagcaca gaccattctt agccattgat tttccttctg
     8821 aatcaaccgg aatctgttta agacactttt tacactttac ctcacattca atcataatta
     8881 taaaactcct tccttcatat gatttattat agggtttttg tctgatatga aaaaactttt
     8941 agataccact actattgctt atagctgttt taactatttt gactatgttg tccaagtcgc
     9001 tttccgttgc tatgtattgt ggaaaaatca gaattgatga tgtaatgtct tttgtatagg
     9061 agattcctgc aaaacagcct aaagaataaa tggttgatat aactttaata agctgctcat
     9121 gacttatagg attgtttgtt tctctatccg ctaaatcaat ggctgccatt aacccgtgta
     9181 tgcgtatttc aaaaaccata ctcagattag aaaggtcatc attcaatatt tttttgaaat
     9241 aagtactttt tgcgtttaca acttctaaaa tattatctct ttccattttg tctattgtcg
     9301 ccaatgcagc agccaaacaa ataggattag cattttgtgt agataaatgg aataatatct
     9361 gattttcctt taaaaaagca ctttctattt tttcagaaat acatactgca cccagcggca
     9421 gatagccatt atttatacct tttgacatag taataatgtc cggttttaaa tcaaattttt
     9481 gaaatctgaa cattgtgcct gttcttccga aacccgtagc aacttcatca caaacaaaga
     9541 gaacattatt ttctctgcaa aaactgttta tcctggacag atactcttca aataatggaa
     9601 tgactccacc agaagctaat atcggttcta caataatagc acatagttta tctttgttat
     9661 tttgtaaagt ttcctctagt ttttgcataa ccttattttt ttcgtctatt gtcatttctt
     9721 ctgttttaca tcttcttgaa aatggtagaa ccaatttaat aaagccatca accattggtc
     9781 cataaccctg tttttcattt tcatcataga aagacgctga cattgatcca taataacttc
     9841 catgatagga ttctgaaatt actgctatat gatattgctt tttgcctatc aggttgttat
     9901 atttacgcga aatcttgatg gcagcttcta tggcttctga accggaacaa gtaaaaagta
     9961 cttttgttat ttcatcgtgt gttatactac atagtttatc tgcaagttca gatgcttttg
    10021 ggttccccag agtgatagga ttaatatacg gtagttcctc aatctgtgtc ttaatagctt
    10081 tcttaatatc gttatcacta taaccataat tcatattcca cagaccgcta tacgcatctt
    10141 gaaaccttct accggagtca tcgtatagaa atatcccctc gccttttact atattaatta
    10201 cgttctcacc atcagccctg ttgaatggtc tccagtatgt gctccccatt atttttccgt
    10261 cccctttctt aaaacccaga atcttgcaat tgatactagt tccaaaaaaa ctttgctatt
    10321 tccttaatat taaagacctc cagcaaacaa aagcaacaat tatcgttaac ataaatcaaa
    10381 tactttttaa acggatattc caataatgaa aatctctgtt caccaaaaat ggcattaagt
    10441 tcatttttgt ttaaataacc actccagagt atttctcttt ttgtttcgtt atcttttatt
    10501 attttgccta aaggagtttt actacggagt agagcgtttt taatctcatc agaaatgttg
    10561 tcgaatgaag ctattacaat attgtcagag aagatattgc catgactatg aagtgatact
    10621 gtacgtttta gatatttttc agacttttta agaggtgaaa tgtaatcata ataattttct
    10681 gaaagatcct tttcctcagt taccttctga cttgtgacat taatctctac ttctctccct
    10741 gtcattatct ccagcaacct agtggtagaa ctcttggaca ctagtaggat atccgccaac
    10801 tttctttcag tctgagttat ttcagtcagc agcatccggt ttgtggaatt attcatctgc
    10861 atcat
//
