BGC0001902 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001902 (Myxococcus virescens)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS	1	37,175	High	bengamide	NRPS:Type I+PKS

BGC0001902 - Region 1 - NRPS,T1PKS

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Location: 1 - 37,175 nt. (total: 37,175 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 37,176
Label		BGC0001902

Tailoring functions

Oxidoreductases: dehydrogenase

benF: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

benH: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

Hydrolases: peptidase phosphatase

benE: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

benI: methionine aminopeptidase

smcogs: SMCOG1172: methionine aminopeptidase

Ligases: ligase synthase

benA: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

benB: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

benC: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

benD: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001902.3	1.84	NRPS, PKS	bengamide	Myxococcus virescens
BGC0001132.4	0.69	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.68	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001758.4	0.67	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000107.3	0.66	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001902.3	0.99	NRPS, PKS	bengamide	Myxococcus virescens
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.67	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000107.3	0.66	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 37175:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (ohmal) + (ohmal) + (Lys)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 9373 - 37175:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (ohmal) + (ohmal) + (Lys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

benA: mmal

PKS_AT (541..825): mmal

ATSignature: Ethylmalonyl-CoA, Malonyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 58.3%; Malonyl-CoA: 58.3%; Methylmalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 89.9
Isobutyryl-CoA: 85.7
Malonyl-CoA: 84.1
Methoxymalonyl-CoA: 80.2
Propionyl-CoA: 61.6
Ethylmalonyl-CoA: 56.6
3-Methylbutyryl-CoA: 49.5
inactive: 48.3
2-Methylbutyryl-CoA: 43.0
Benzoyl-CoA: 41.1
trans-1,2-CPDA: 40.6
Acetyl-CoA: 34.3
CHC-CoA: 23.9
fatty_acid: 7.1

benB: mal

PKS_AT (564..868): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 62.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 123.9
inactive: 70.3
Methoxymalonyl-CoA: 62.7
Methylmalonyl-CoA: 48.4
Ethylmalonyl-CoA: 43.5
Propionyl-CoA: 30.6
Isobutyryl-CoA: 18.3
trans-1,2-CPDA: 15.5
Benzoyl-CoA: 14.4
2-Methylbutyryl-CoA: 13.0
Acetyl-CoA: 12.4
CHC-CoA: 12.2
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

benC: mal

PKS_AT (537..744): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 20.8
inactive: 17.9
Methoxymalonyl-CoA: 4.9
Propionyl-CoA: 4.0
Methylmalonyl-CoA: 3.3
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

benD: Lys

Search NORINE for peptide: strict or relaxed

AMP-binding (503..906): Lys

nrpys: Lys

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DNESGGTVAK
Nearest Stachelhaus code(s):: Lys DNESGGTVAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor