LOCUS       BGC0001920             11868 bp    DNA     linear   BCT 16-OCT-2018
DEFINITION  Streptomyces sp. M-271 DNA, MS-271 biosynthetic gene cluster.
ACCESSION   BGC0001920
VERSION     BGC0001920
KEYWORDS    .
SOURCE      Streptomyces sp
  ORGANISM  Streptomyces sp.
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-08 05:23:22
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 10367
            Orig. end    :: 22235
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            complement(1..693)
                     /gene="mslR1"
     CDS             complement(1..693)
                     /codon_start=1
                     /gene="mslR1"
                     /gene_functions="regulatory (smcogs) SMCOG1016:LuxR family
                     DNA-binding response regulator (Score: 112.1; E-value:
                     3.5e-34)"
                     /gene_kind="regulatory"
                     /product="LuxR family transcriptional regulator"
                     /protein_id="BBD82036.1"
                     /transl_table=11
                     /translation="MRINLFILSEDPVSLAGLEAIVEQDDSVNLIGTAVDESHGLRCLE
                     NNPLRPDVVIIAEPPSKRAICRNVAWIVSLYRQDKSRPQIVVVSQNDYDDVIVSALRLG
                     VNGYLARIASPEELFQALHIVARGGAAFSPTIAARFSRFFATIQRLPEPPEFSQLTSRE
                     TEILELLADGMNNRQIARRLFLAEKTVRNYVSRIFTKLEVHDRAAAVLRARDAGFGDQA
                     DAVLPQHA"
     misc_feature    1..11868
                     /note="MS-271 biosynthetic gene cluster"
     subregion       1..11868
                     /aStool="mibig"
                     /contig_edge="False"
                     /label="RiPP"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11868
                     /contig_edge="False"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            844..1977
                     /gene="mslG"
     CDS             844..1977
                     /codon_start=1
                     /gene="mslG"
                     /gene_functions="regulatory (smcogs) SMCOG1048:sensor
                     histidine kinase (Score: 211; E-value: 5.3e-64)"
                     /gene_kind="regulatory"
                     /product="sensor histidine kinase"
                     /protein_id="BBD82037.1"
                     /transl_table=11
                     /translation="MGDVSRRRLILLDGAAALGFALLPQLTLVRPDSGGAPGGLAVSLL
                     SLATALPLAVRRLWPVPVFAIVLTASCVALAAGLGPAVFLSAAYALYLVATTRRRQLGR
                     SAVIVGGASTAGAALLTVTGGQHYTGGTLAVQLLFGLLVLGATWAAGTAVRERHESLRR
                     TIVQAAEQAKVEERMRIARDIHDVVTHSVGLIAIKAGVANHVIATHPEEAGEALTVIED
                     ISRRALRDMRATLKVLRREDDAQQQDLQPVPGLSDLPSLVRTAEAAGVSVDLRSDCVEE
                     PPDGVALTAFRIVQEALTNVVKHAAPTRCLVSVTAQDGVLTIGVTDDGPGPGHRPTVPG
                     GAMGLVGMRERAVAHGGTLTAGPRPGAGFRILATLPY"
     gene            2124..3047
                     /gene="mslD1"
     CDS             2124..3047
                     /codon_start=1
                     /gene="mslD1"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 169.5; E-value:
                     1.6e-51)"
                     /gene_kind="transport"
                     /product="ABC transporter"
                     /protein_id="BBD82038.1"
                     /transl_table=11
                     /translation="MIDVQNLTKRYGPATVVDGLTFTVRPGAVTGFLGPNGAGKSTTLR
                     MMLGLTRPDTGTARIDGHAYGDLAHPLRRIGALLETSAPHRGLTARDHLLWLAQSNRIA
                     RGRVAEVLEAVGLADAARRRTGTFSLGMGQRLGLAAALLGDPPVLVLDEPVNGLDTEGI
                     RWLRDLLRSMAAEGRTVLISSHLMTEMALVADHLVVISRGRLLADTGMSDFIERHGRSY
                     VRVRTAEPDRLGRELEGRGATVSRVPGGGLDVVGMAAADVSRIAAAGGFPLDELATHAG
                     SLEETFLDVVGEGQQVHGPEGRNTHV"
     gene            3040..3792
                     /gene="mslD2"
     CDS             3040..3792
                     /codon_start=1
                     /gene="mslD2"
                     /product="ABC transporter"
                     /protein_id="BBD82039.1"
                     /transl_table=11
                     /translation="MSETLAAVRAETTKLRGIRGTRISLLLFAAVSVLIAALDGWSAKN
                     ALESDNPSLRSDFTPEQAGLDGILYGQLALIVFGVLVVTSEYTSGMIRVSLLAVPRRGR
                     LYAAKTVVTALAAVAVSVPVTVLGYLVTQAALGSHGSSLGASGVPRALAGAVVYLTLMC
                     LFAAGIAAIARNAVVPLAVLLPMVLAGTHILSLIGATKEMARYFPDQAGNELLTVRSPH
                     TAFGFTVLLAWTVAALGWGWVRQRRWDS"
     gene            3908..4036
                     /gene="mslA"
     CDS             3908..4036
                     /codon_start=1
                     /gene="mslA"
                     /product="MS-271 precursor peptide"
                     /protein_id="BBD82040.1"
                     /transl_table=11
                     /translation="MSAIYEPPMLQEVGDFEELTKCLGVGSCNDFAGCGYAIVCFW"
     gene            4110..5951
                     /gene="mslC"
     CDS             4110..5951
                     /codon_start=1
                     /gene="mslC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177:asparagine synthase (glutamine-hydrolyzing)
                     (Score: 125.7; E-value: 3.5e-38)"
                     /gene_kind="biosynthetic"
                     /note="lasso peptide isopeptide bond-forming cyclase"
                     /product="asparagine synthetase"
                     /protein_id="BBD82041.1"
                     /sec_met_domain="Asn_synthase (E-value: 5.6e-40, bitscore:
                     127.9, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFVVLPDCPAGAAAVGRLRATRRVDHASGRPWIVGDWPEAEAVV
                     VEAGPRRMVVLGHSRLDETAAAAALGRLRSLHDVDSIASRLPGAVHLAVSLDGRTRVQG
                     SAVGVRQIFTAVVDGVTVAASGVEPLLRLTGAGLDETVLAARLLAPGGPPWPLAQRPVR
                     RGVEALTTGHWLELDTDGRARQTRWWELPEPSLTLAQGAAAVRSALDDAITSRVAAGGT
                     LSADLSGGLDSTSLCFLAHAAGADLVTYHVMPIDSANADTMWAHRAAECLPAARHHTLS
                     ADRAENLFDVGYTADLVGAAPEGPSTWASGLAHIQDLAKRATAEGATLHLTGFGGDELF
                     GRMPACAWSLARATPVGGLRLVNRYRLANRWPWRATVRSLLDRSTFTQNLGRVAARIDA
                     PPPPVDEPDFGWVFAPRMPAWATPDAVAAVRALLTDAATEGPGPLDADRARHQALASLV
                     FEGTTVRQVNTALGDTGITWDAPFLDDRVVEAALATRIDQRLLGGRFKPLLTSAARGLV
                     PADILGRRDKGEFSAEAFRGLARNRARILELCEDSQLARLGLIDPAAFRSAVLNPGPMS
                     HHLQPIDTTVACESWLRTHPETYPPPPARNTPTGEHR"
     gene            5948..6211
                     /gene="mslB1"
     CDS             5948..6211
                     /codon_start=1
                     /gene="mslB1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Lasso_Fused_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /note="lasso peptide biosynthesis B1 protein"
                     /product="PqqD family protein"
                     /protein_id="BBD82042.1"
                     /sec_met_domain="PF05402 (E-value: 6.4e-14, bitscore: 42.0,
                     seeds: 461, tool: rule-based-clusters)"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 3.5e-26,
                     bitscore: 81.1, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="Lasso_Fused_RRE (E-value: 5.6e-11,
                     bitscore: 32.4, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLTLARDVTLTVVDSGAVLLDGRRGRYWQLNHSGAGVLRQLLDG
                     TAPDAAAAGLCAAAPVSDDQARQDVQALIDALSAAKLVEVAS"
     gene            6208..6678
                     /gene="mslB2"
     CDS             6208..6678
                     /codon_start=1
                     /gene="mslB2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /note="lasso peptide biosynthesis B2 protein"
                     /product="cysteine protease"
                     /protein_id="BBD82043.1"
                     /sec_met_domain="PF13471 (E-value: 6.2e-27, bitscore: 83.9,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPAVAEQAPRLPWYRQLAPRCAAGAARLLVRLPPARLHRVLGV
                     VSKGSRPAGYAEVARARRSVVSVSTRCAGLGCLQRSVATVLLCRAHGRWADWCTGFRTE
                     PFGAHAWVEAEGRPVDEPGELSVFRTVLAVRRPDGRRSTSDRPLRPSRGSRS"
     gene            6675..7640
                     /gene="mslD3"
     CDS             6675..7640
                     /codon_start=1
                     /gene="mslD3"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 207.9; E-value:
                     2.8e-63)"
                     /gene_kind="transport"
                     /product="ABC transporter"
                     /protein_id="BBD82044.1"
                     /transl_table=11
                     /translation="MTAIRAEGLYAYYGTAPAVNGLDLTVPPGSVYGFLGPNGAGKTTT
                     INMLTTLLRPTAGRAEVAGFDVAARPAEVRRRIGIVFQESTLDLDLTAAENLRFQADLC
                     GLSRRASRDAIASMLDLMDLSERRRVPVRQFSTGLRRRLEIARGLLAEPSVLFLDEPTT
                     GLDAQTRAAVWEHLERLRRERGITVFVTTHQLDEAEHCDRIAIIDRGKVVTEGTPADLK
                     SVIGADLVVLRTDDDQRAAAVLGDRFGLPAEPTPDGLLLRVERAAALVPRLCTELGVTV
                     REVAIAPPTLDDVFLHHTGLAIRESPTGPRTLGNLGEGLR"
     gene            7637..8506
                     /gene="mslD4"
     CDS             7637..8506
                     /codon_start=1
                     /gene="mslD4"
                     /gene_functions="transport (smcogs) SMCOG1065:ABC-2 type
                     transporter (Score: 124.7; E-value: 5.8e-38)"
                     /gene_kind="transport"
                     /product="ABC transporter"
                     /protein_id="BBD82045.1"
                     /transl_table=11
                     /translation="MSRTDTAPAALGDVSAAPSPTDRSRNAARPVLLLWRREMTRLRHN
                     PVRLAMGLVTPLLFLVVLGTGLDAASSSLGKAQLNDYRAYLFPGTLVMSVQAPAIAVGI
                     SLVWDRRLGVLRQMLVAPFPRASIVFGLAFGGATTGAVYGLMVLSVGGIASIRYTPMLL
                     VVLLELLLVSLMFTALGLLAAVTIRQVDTFQVVVNLSLMPLMFFSGAMFPPNGLPGWLD
                     TVVKLNPLTYGVDAVRRTLPGPSVLTSEQTRLMLGDWHPPVAAELGVLAALTAVALGLA
                     GYRFSRTS"
     gene            8503..9048
                     /gene="mslE"
     CDS             8503..9048
                     /codon_start=1
                     /gene="mslE"
                     /product="DoxX family protein"
                     /protein_id="BBD82046.1"
                     /transl_table=11
                     /translation="MSRGGQETVDASTTDVTTTAAVRATTGHWAGAARLVARLLLAAVL
                     AYAGLVKIGDLTEAGRTVALYRIVPADSAQLVGGVLPFVEVALALLLAAGLATRAAAAG
                     AAVLLVAYAAAIASVWARGMSIDCGCFGGGGTLSGGAARGYALDLARDLLLLGAAALLI
                     RNPRTRYALDGWVLDPKE"
     gene            9045..9785
                     /gene="mslF"
     CDS             9045..9785
                     /codon_start=1
                     /gene="mslF"
                     /product="protein disulfide isomerase"
                     /protein_id="BBD82047.1"
                     /transl_table=11
                     /translation="MRGMTSTQTTDATVREMVHRRHRRRRTVVVSLVAALVVVAAALVG
                     AGLVRANNTAPGKAPSRVPAGLAADKSGVAASTGAVRVDVYLDYLCPECRRTERALTTA
                     LDSLRAHGGVSVVYHPVAFLDSRSAPAGYSTQAASAAACAADAGRFEQYSTVLFSKQPA
                     EQGPGLSEAQLIAAGRDAGITAASFARCVEDAPYLPWVRYVSDLAASRKVALTPTVMVA
                     GRRVDVTGSDPGGALTRAVTAARR"
     gene            9782..11104
                     /gene="mslH"
     CDS             9782..11104
                     /codon_start=1
                     /gene="mslH"
                     /note="CapA family protein"
                     /product="hypothetical protein"
                     /protein_id="BBD82048.1"
                     /transl_table=11
                     /translation="MTRLTVALSGDCMATRGAVISSDPAAGRLHELLHGADFAVTNLEV
                     VPSDGRGHPVHNAAGGGCLIADSGVLDEITAAGFTVLGCANNHAMDLGTEGVLGTVDLL
                     RSRRIPFAGIGADLTTARRPAYVDRPGGSLALLACTATFLPGQEAADPSPELLGRPGLS
                     PLRHSATLRVTAPQMDVLRTIDAETGLRARRAEARTLLGVDPALLGPDRLSLFGTRFLT
                     ADEPGFTTECDPRDLDEISRWVGEARLRADLVVVSVHSHEPGPTPETPGEFLRVFAHRV
                     IDEGADVVVGHGPHFLRGVELYRNRPIFYSLGNIVSQIELTDRVSAEDYAKVTTDRPPT
                     PGRYYDRLSGHGTRLFAPHRRYWQSLVPVLTFDDGTLVTAHLHPVDLGFALPVHRRGRP
                     RLADQAEAKEILTEVARLSESYGTTVRAGAGGAAELLLDVA"
     gene            11194..11868
                     /gene="mslR2"
     CDS             11194..11868
                     /codon_start=1
                     /gene="mslR2"
                     /gene_functions="regulatory (smcogs) SMCOG1016:LuxR family
                     DNA-binding response regulator (Score: 190.2; E-value:
                     4.6e-58)"
                     /gene_kind="regulatory"
                     /product="LuxR family transcriptional regulator"
                     /protein_id="BBD82049.1"
                     /transl_table=11
                     /translation="MAESVRVLIADDQALLRGSFRVLVDSTPGMEVVGEAGDGAMAVRL
                     ARELAPDVVLMDLRMPVLDGIAATREICSDETLTGVRVLALTMFDMDEYVYPALQAGAS
                     GFMLKDASPSDLVDGIRVIATGEGVLAPTVTRRVIANFSRRGETPRPAPRLVGLTKREH
                     EVLVQIANGLSNAEISERLVISMPTVKTHVGSLLAKLHARDRAQLVIIAYESGLAERGM
                     PT"
ORIGIN
        1 tcaggcgtgt tggggcagta cggcgtcggc ctggtcgccg aaacctgcgt cgcgtgcgcg
       61 cagtacggcg gcggcccggt cgtgcacctc cagtttggtg aagatgcggg agacgtagtt
      121 gcgtacggtc ttctccgcga gaaagaggcg ccgggcgatc tggcggttgt tcatgccgtc
      181 ggcgagcagt tccagtatct ccgtctctct gctggtcagt tgggagaact cgggcggctc
      241 ggggagtctt tgtatggtgg cgaagaacct gctgaaacga gccgcgatcg tcgggctgaa
      301 cgcggcgccc ccgcgggcca cgatgtgcag cgcctgaaac agttcctccg gggacgcgat
      361 ccgcgcgaga tacccgttca cgccgaggcg gagggcggag acgatcacgt cgtcgtagtc
      421 gttctgcgac accacgacga tctgcggccg tgacttatcc tggcggtaca gggagacgat
      481 ccacgcaacg ttcctgcaga tggcgcgttt tgacggtggc tcagcgatga tcaccacgtc
      541 cggccggagc gggttgttct ccagacatcg aaggccgtgg gattcatcga ccgccgtgcc
      601 gatgaggttg acgctgtcgt cctgttcgac gatcgcttca agtccggcca gggacaccgg
      661 gtcctcactg aggatgaaga gattgatccg catagctttg cacccccgtg gtaagcgttg
      721 tacgcctgca aagacggtgc cagcctaagc agactaatcg gacggactac accgacttga
      781 gcggtcatgg tccggttccg gccgcgttgg acgtccggct gatcagacac ataggttcgg
      841 gacatgggag atgtctcgcg ccgacgcctg atcctcctcg acggggcggc ggccctcggg
      901 ttcgccctgc ttccgcaact gacgctggta cggcccgaca gcggcggcgc gcccggtggg
      961 ctcgccgtat cgctgctctc gctcgccacc gcgctgccgc tcgccgtacg gcggctgtgg
     1021 cccgtcccgg tgttcgcgat cgtcctcacc gcgtcctgcg tggcgctggc cgccggtctc
     1081 ggccctgccg tcttcctctc cgcggcctac gccctgtacc tggtcgcgac gacacggcga
     1141 cggcagctgg ggcggtcggc cgtgatcgtc ggcggggcga gcacggcggg cgcggccctg
     1201 ctgacggtga cgggcgggca gcactacacg ggcggcaccc tggcggtgca actgctgttc
     1261 gggctattgg tgctgggggc gacgtgggcc gcggggacgg ccgtacggga gcgacacgag
     1321 agcctgcggc ggaccatcgt gcaggcggcc gagcaggcca aggtcgaaga acgcatgagg
     1381 atcgcccggg acatccacga cgtcgtcacc cacagcgtgg gcctgatcgc gatcaaggcc
     1441 ggggtcgcca accacgtcat cgccacccac cccgaggagg ccggagaggc cctcaccgtc
     1501 atcgaggaca tcagccgcag ggcgctgcgc gacatgcggg ccaccctcaa ggtgctgcgc
     1561 cgagaagacg acgcccagca gcaggacctc cagccggtcc ccggactgtc ggacctgcct
     1621 tccctggtcc gtacggccga ggcggcgggc gtcagcgtcg acctgcgctc cgattgcgtg
     1681 gaggaaccac ccgacggggt cgcgctgacc gcgttccgga tcgtccagga ggcgctgacc
     1741 aacgtcgtca aacacgctgc cccgacccgc tgcctggtca gcgtcaccgc gcaggacggt
     1801 gtgctgacga tcggcgtcac cgacgacggc cccggacccg ggcaccggcc gaccgttccc
     1861 ggcggcgcca tggggctcgt cgggatgagg gaacgggccg tcgcgcacgg cgggaccctc
     1921 acggcgggcc cccgccccgg cgcgggcttc cggatcctcg cgacactgcc gtactagtgc
     1981 cacgccaacc gccatacgtc ccgccccccg catatggctg aaacggtctc ataccccggt
     2041 atggggccgg tccgcgaaac cggcccgcac cgtcccgacc ccggtccgat gtgtggcgcg
     2101 gccgccgcgg ccagactcgt cgcgtgattg acgtccagaa cctgaccaag cggtacgggc
     2161 cggccaccgt cgtggacggc ctgaccttca cggtgcggcc cggagccgtc accggcttcc
     2221 tcggccccaa cggcgccggg aagtcgacca ccctgcggat gatgctgggc ctgacccgcc
     2281 ccgacaccgg aaccgcgcgg atcgacgggc acgcctacgg cgacctggcc caccccctcc
     2341 gccgcatcgg cgccctgctg gagacctcgg ccccgcaccg cggcctgacc gcgcgcgacc
     2401 atctgctctg gctcgcccag agcaaccgga tcgcccgcgg ccgggtcgcc gaggtcctgg
     2461 aggcggtcgg gctcgcggac gcggcccggc gccggaccgg caccttctcc ctcggcatgg
     2521 gccagcggct gggcctggcc gccgcgctcc tcggcgaccc gcccgtgctg gtgctcgacg
     2581 aaccggtcaa cggcctggac accgagggca tccgctggct gcgcgacctg ctgcgctcga
     2641 tggccgccga gggccgtacc gtcctcatct ccagccatct gatgaccgag atggccctgg
     2701 tcgccgatca cctggtcgtc atcagccggg gccggctcct cgcggacacc gggatgtcgg
     2761 acttcatcga gcgccacgga cggtcgtacg tccgggtccg caccgccgaa cccgaccggc
     2821 tcggcaggga gttggaaggt cgcggcgcca cggtgagccg cgtccccggc ggcggcctgg
     2881 acgtcgtggg catggcggcc gcggacgtca gccggatcgc cgcggcgggc ggcttcccgc
     2941 tcgacgaact cgccacccac gccgggtcgt tggaggagac cttcctcgac gtcgtcggag
     3001 agggccaaca ggtccacggt ccggaaggca ggaacacgca tgtctgagac cctcgcggcc
     3061 gtccgcgccg agaccaccaa gctgcgcggc attcgcggca ccaggatctc gctgctgctg
     3121 ttcgccgcgg tcagcgtcct catcgccgcc ctcgacggct ggtccgcgaa gaacgcgctg
     3181 gagtccgaca acccgagcct gcgttccgac ttcaccccgg aacaggccgg tctcgacggc
     3241 atcctctacg gccagttggc gctgatcgtg ttcggcgtcc tcgtcgtcac cagcgagtac
     3301 acgtccggca tgatccgtgt ctccctgctc gccgtccccc ggcgcggacg gctctacgcg
     3361 gcgaagacgg tcgtcaccgc cctggcggcg gtggccgtct ccgtccccgt caccgtcctc
     3421 ggctacctgg tcacgcaggc ggctctcggg tcccacggtt cctcgctcgg cgcgagcggt
     3481 gtgccgcgcg ccctggccgg ggcggtcgtc tatctgaccc tgatgtgcct gttcgcggcg
     3541 ggtatcgcgg cgatcgcccg caacgccgtc gtaccgctgg ccgttctgct gccgatggtg
     3601 ctcgccggga cgcacatcct gtccctcatc ggggcgacca aggagatggc ccggtacttc
     3661 cccgaccagg cgggaaacga actgctcacc gtccggtccc cgcacacggc cttcggcttc
     3721 acggtgctgc tcgcctggac cgtcgccgcc ctcggatggg gctgggtgcg ccagcgccgg
     3781 tgggacagct gacaggaccg gcatgggaca actgccccat gccggtggga cagtccgccc
     3841 cgtagcttcc gtgacagatc acaggccgaa caaggtcggc ctgaccgatc cgaaacggga
     3901 ggacaccatg tccgcgatct acgagccccc catgctccag gaagtcggcg acttcgagga
     3961 gctcacgaag tgcctcggcg tcggaagctg caacgacttc gccggctgcg gctacgcgat
     4021 cgtctgcttc tggtgatcac gtccggtgcc ggtgcgttca cgcgcaccgg caccacccgg
     4081 ggtgacgagg agacggggaa cgaggcgaca tggaattcgt ggttcttccg gactgcccgg
     4141 ccggtgccgc ggccgtcggc cggctgcggg cgacgcggcg cgtcgaccac gcgtcggggc
     4201 ggccctggat cgtcggcgac tggcccgagg ccgaggccgt cgtcgtcgag gcgggcccac
     4261 ggcggatggt cgtactggga cacagccggc tcgacgagac cgccgcggcg gccgcgctcg
     4321 gccggctgcg ctcgctgcac gacgtggact cgatcgcgtc ccggctgccg ggagccgtcc
     4381 acctggcggt gtcactggac ggcaggacca gggtgcaggg ttcggccgtc ggcgtacgac
     4441 agatcttcac cgccgtcgtc gacggggtga ccgtcgccgc gagcggggtg gaacccctgc
     4501 tgcggctgac cggcgccggc ctcgacgaga ccgtgctcgc cgcccgcctg ctggcgccgg
     4561 gcggaccacc ctggccgctc gcccagcgcc ccgtccgccg gggtgtcgag gcgctcacca
     4621 ccggccactg gctggaactg gacacggacg gccgggcccg gcagacccgc tggtgggaac
     4681 tcccggagcc gtccctcacg ctcgcgcagg gcgcagccgc cgtccgttcg gcgctggacg
     4741 acgcgatcac cagccgggtc gccgcgggcg gcaccctcag cgccgacctg tccggcggtc
     4801 tggactccac ctcactgtgc ttcctcgcgc acgcggccgg cgccgacctg gtcacgtacc
     4861 acgtgatgcc gatcgacagc gccaacgcgg acacgatgtg ggcccaccgg gccgcggagt
     4921 gcctgcctgc ggcccggcac cacacgctgt ccgccgaccg cgccgagaac ctgttcgacg
     4981 tcggctacac cgccgacctc gtgggcgcgg ccccggaggg tccctcgacc tgggcctccg
     5041 gactcgccca catccaggac ctggccaagc gggccacggc ggagggcgcc acactgcacc
     5101 tgaccggctt cggcggtgac gagctgttcg gccggatgcc cgcctgcgcc tggtccctgg
     5161 cccgggccac accggtcggc gggctgcggc tggtcaaccg ctaccggctg gccaatcgct
     5221 ggccgtggcg ggcgaccgta cgctcgctcc ttgaccgctc gacgttcacg cagaacctcg
     5281 gtcgggtcgc cgcccgcatc gacgccccgc ccccgcccgt cgacgagccc gacttcggct
     5341 gggtgttcgc gccccgcatg ccggcctggg cgacccccga cgccgtggcc gcggtccgcg
     5401 cccttctcac cgacgccgcc accgagggac ccgggccgct ggacgccgac cgggcccggc
     5461 accaggcgct cgcctcgctc gtcttcgagg ggaccaccgt ccgccaggtc aacaccgccc
     5521 tcggggacac cggcatcacc tgggacgcgc ccttcctcga cgaccgggtg gtggaggcgg
     5581 ccctggccac ccggatcgac cagcgcctgc tcggcgggcg gttcaagccg ctgctcacct
     5641 cggccgcacg gggtctcgtc cccgcggaca tcctgggccg ccgtgacaag ggcgagttca
     5701 gcgcggaggc gttccggggc ctggcccgca accgggcccg gatcctggag ctgtgcgagg
     5761 actcccagct cgcccggctc ggcctcatcg acccggcggc cttccggtcc gcggtgctga
     5821 acccggggcc gatgtcccac catctccagc cgatcgacac cacggtggcg tgcgagagct
     5881 ggctgcggac gcatccggag acgtaccccc cgccacccgc ccggaacacg cctacgggag
     5941 aacaccgatg aagctgaccc tcgcccgcga cgtcaccctc accgtcgtcg actccggggc
     6001 cgtgctgctc gacgggcgcc gcggccgcta ctggcaactg aaccactccg gcgcgggcgt
     6061 cctgcgccaa ctgctcgacg gaacggcgcc cgacgcggcc gccgccggcc tctgcgccgc
     6121 ggccccggtc agcgacgacc aggcacggca ggacgtccag gccctcatcg acgcgctcag
     6181 cgcggccaag ctcgtggagg tggcctcgtg accacccccg ccgtggccga acaggccccg
     6241 cggctgccct ggtaccggca gctcgccccc cggtgcgccg cgggggcggc ccgtctgctg
     6301 gtccggttgc cgccggcccg actgcaccgc gtgctgggcg tggtcagcaa ggggtcccgc
     6361 cccgccggat acgccgaggt ggcgcgggcc cgccggtccg tcgtctcggt cagcacccgc
     6421 tgcgcgggac tcggttgcct ccagcgttcc gtggccaccg tcctgctgtg ccgggcacac
     6481 ggcaggtggg ccgactggtg cacgggattc agaaccgaac cgttcggcgc gcacgcctgg
     6541 gtggaggccg aggggcggcc ggtggacgag cccggcgaac tcagcgtgtt ccgcacggtc
     6601 ctggcggtcc gccgcccgga cggacgccgg agcacctccg accgtcccct ccgcccctcc
     6661 cgagggagcc gctcatgaca gcgatccggg ccgagggcct ctacgcgtac tacggcaccg
     6721 caccggccgt gaacgggctc gacctgaccg tgcccccggg cagcgtctac ggcttcctcg
     6781 gaccgaacgg cgcgggcaag accaccacca tcaacatgct gaccaccctg ctgcggccca
     6841 ccgcgggccg tgcggaggtg gccggcttcg acgtcgccgc ccggcccgcc gaggtccgcc
     6901 gccgtatcgg catcgtgttc caggagtcga ccctcgacct ggacctcacc gccgccgaga
     6961 acctccgctt ccaggccgac ctgtgcggcc tgtcccgccg cgcgtcccgc gacgcgatcg
     7021 cctcgatgct cgacctgatg gacctctccg agcgccgcag ggtgcccgta cggcagttct
     7081 ccaccggact gcgccgccgc ctcgagatcg cccgtggcct gctcgccgag cccagcgtgc
     7141 tgttcctcga cgagccgacg accggactgg acgcccagac ccgcgccgcc gtctgggagc
     7201 acctggaacg gctgcgccgg gaaaggggca tcacggtctt cgtcaccacc catcaactgg
     7261 acgaggccga gcactgcgac cggatcgcca tcatcgaccg gggcaaggtg gtcacggagg
     7321 gcacaccagc ggacctcaaa tccgtcatcg gggccgacct cgtcgtcctg cgcaccgacg
     7381 acgaccagcg cgccgccgcc gtcctcggcg accggttcgg cctcccggcg gagcccactc
     7441 cggacggtct gctgctccgg gtcgagcgcg cggcggccct ggtgccccgc ctgtgcaccg
     7501 aactcggcgt gaccgtacgc gaggtcgcca tcgccccgcc cacgctcgac gacgtcttcc
     7561 tgcaccacac cggtctcgcc atccgggaga gcccgaccgg cccgcgcacg ctcggcaacc
     7621 tcggggaagg actgcgatga gccggaccga caccgcaccc gccgcactcg gcgacgtcag
     7681 cgccgcaccc tcaccgaccg accggtcccg caacgcggcg cgccccgtcc tgctgctctg
     7741 gcggcgggag atgacccggc tgcggcacaa ccccgtgcgc ctggccatgg gactcgtgac
     7801 acccctgctg ttcctcgtcg tcctcggcac cggcctcgac gcggcgtcgt ccagcctcgg
     7861 caaggcccaa ctgaacgact accgggccta cctgttcccc ggcacgctgg tcatgtccgt
     7921 gcaggcgccg gcgatcgcgg tgggcatctc gctggtgtgg gaccgcaggc tgggggtgct
     7981 gcgccagatg ctcgtggcgc cgttcccgcg cgcgtccatc gtgttcggac tggccttcgg
     8041 cggcgccacc accggcgcgg tctacggcct catggtgctg tccgtcggcg ggatcgcgag
     8101 catccgctac acgccgatgc tgctggtcgt cctcctcgaa ctcctgctgg tctccctcat
     8161 gttcaccgcg ctcgggctgc tcgccgccgt caccatccgg caggtcgaca ccttccaggt
     8221 cgtggtgaac ctgagcctga tgccgctgat gttcttctcc ggcgcgatgt tcccgcccaa
     8281 cggcctgccc ggctggctcg acaccgtcgt caagctcaac ccgctgacgt acggcgtcga
     8341 cgcggtccgc cggaccctgc caggaccgag cgtgctcacc tcggagcaga cccggctgat
     8401 gctcggcgac tggcacccgc ccgtggccgc cgaactgggt gtcctggccg ccctcaccgc
     8461 ggtcgcgctg ggcctcgccg gctaccggtt ctcccgtacg tcatgagccg gggaggacag
     8521 gagacggtgg acgcgagcac cacggatgtg accacgacgg ccgccgtccg ggccacgacc
     8581 gggcactggg cgggcgcggc gcggctcgtg gcccggctgc tcctggcggc ggtcctggcc
     8641 tacgccggtc tggtgaagat cggggacctc acggaggccg ggcggacggt cgcgctctac
     8701 cgcatcgtgc ccgccgactc ggcccaactc gtgggcggcg tcctgccgtt cgtcgaggtg
     8761 gcgctcgcgc tgctgctcgc ggccgggctg gccaccaggg cggcagcggc gggcgcggcc
     8821 gtgctgctgg tcgcctatgc ggcggccatc gcctcggtgt gggcacgggg catgtccatc
     8881 gactgcggtt gtttcggcgg cggaggcacg ctcagtggtg gcgccgcacg cggctacgcg
     8941 ctcgacctcg cgcgcgatct gctgctgctc ggcgcggccg ccctcctgat ccggaatccg
     9001 cgcacccgat acgcgctgga cggctgggtc ctggacccga aggagtgagg ggcatgacga
     9061 gcacacagac aacggacgcc acggtgcggg agatggtgca ccgacggcac cggcgacggc
     9121 gcacagtggt ggtgtctctg gtggccgccc tggtggtggt cgccgccgcg ctggtgggcg
     9181 cgggcctggt cagggcgaac aacacggcgc ccggcaaggc accgagccgc gtaccggccg
     9241 ggctcgccgc cgacaagtcg ggcgtggccg cctccaccgg cgccgtacgc gtcgacgtgt
     9301 acctcgacta cctctgcccc gaatgccgtc gtaccgaacg ggcactgacc accgccctgg
     9361 acagtctcag ggcgcacggc ggggtgagcg tcgtctacca cccggtcgcc ttcctcgaca
     9421 gccgcagcgc acccgcgggc tactcgaccc aggcggcctc cgcggcggcc tgcgcggcgg
     9481 acgcggggag gttcgagcag tactccacgg tcctgttctc gaaacagccc gccgaacagg
     9541 gcccagggct cagcgaggcc cagctgatcg cggcgggccg ggacgccggc atcaccgcgg
     9601 cgtccttcgc ccgctgcgtc gaggacgccc cctacctgcc ctgggtacgg tacgtctccg
     9661 atctcgccgc ctcccgcaag gtggcgctga ccccgaccgt catggtggcg ggccgccgtg
     9721 tcgacgtcac cggctccgat ccgggcggcg cgctgacccg ggcggtcacg gcggcccggc
     9781 ggtgacccgg ctgaccgtgg ctctgtccgg cgactgcatg gcgacacggg gagcggtgat
     9841 ctcctccgac ccggccgccg gacggctcca cgaactcctc cacggcgccg acttcgccgt
     9901 caccaacctg gaggtggtgc ccagcgacgg acgcggacac ccggtgcaca acgcggccgg
     9961 tggcggctgt ctgatcgcgg actccggtgt cctggacgag atcacggcgg ccgggttcac
    10021 cgtgctgggc tgcgccaaca accacgccat ggacctgggc acggagggcg tgctcggcac
    10081 cgtggacctg ctgcggtcga ggcggatccc gttcgccggg atcggcgccg acctcaccac
    10141 ggcgcgccgg cccgcctacg tcgaccggcc gggcggcagc ctggcgctgc tcgcctgcac
    10201 cgcgacgttc ctgcccggcc aggaggcggc cgatccgtcg ccggaactgc tcggacggcc
    10261 gggcctcagc ccgctgcgcc actccgcgac cctgcgggtg acggccccgc agatggacgt
    10321 gctgcggacg atcgacgccg agacgggcct gcgcgcccgc cgggccgagg cccgcaccct
    10381 gctcggggtc gaccccgccc tcctcggacc cgaccggctg agtctcttcg gcacccgctt
    10441 cctgacggcg gacgaaccgg gcttcacgac cgagtgcgat ccgcgcgacc tcgacgagat
    10501 ctcccgctgg gtcggcgagg cccgcctccg cgcggacctc gtggtggtga gcgtgcactc
    10561 ccacgagccc ggcccgacgc ccgagacacc gggcgagttc ctacgtgtct tcgcgcaccg
    10621 ggtgatcgac gagggcgcgg acgtcgtggt cgggcacggc ccgcatttcc ttcgcggtgt
    10681 cgagctgtac cggaacaggc cgatcttcta cagcctcggc aacatcgtca gccagatcga
    10741 actgaccgac cgcgtctccg cggaggacta cgccaaggtc accaccgacc ggccgcccac
    10801 cccgggccgc tactacgacc ggctgagcgg ccacggcacc cggttgttcg ccccgcaccg
    10861 acgctactgg cagtcgctcg tgccggtcct caccttcgac gacggcaccc tcgtgacggc
    10921 ccacctccac ccggtcgacc tcggcttcgc cctgccggtg caccgcaggg gccgcccacg
    10981 cctggccgac caggccgagg ccaaggagat cctcaccgag gtcgcccggc tgtcggagtc
    11041 gtacggtacg acggtccggg cgggggccgg cggcgcggcg gagctgcttc tggacgtggc
    11101 gtgaagaggc ttgtgtggca ggcgagttga cgagagagcc ttgctaaaat gtcatatgtc
    11161 acctacgagc ggacgaggaa ccggggaaca cgcatggccg agtcggtccg ggtactgatc
    11221 gcggacgacc aggcactgct gcgcgggagc ttccgggtcc tcgtcgactc cacacccggc
    11281 atggaggtcg tcggcgaggc cggcgacggc gccatggcgg tccggctcgc ccgggaactc
    11341 gccccggacg tcgtcctgat ggacctccgg atgccggttc tcgacggcat cgcggccacc
    11401 cgggagatct gctccgacga gacgctgacc ggtgtccggg tgctggccct gaccatgttc
    11461 gacatggacg agtacgtcta cccggccctc caggcgggtg ccagcggctt catgctgaag
    11521 gacgcctcgc cgtccgacct ggtcgacggc atccgggtca tcgccacggg cgagggcgta
    11581 ctggccccca cggtcacccg gcgcgtcatc gccaacttct cccggcgcgg cgagacgccc
    11641 cgccccgctc cacgactcgt cggtctcacc aagcgggagc acgaagtcct cgtccagatc
    11701 gccaacggtc tgtccaacgc ggagatctcc gaacgcctgg tcatcagcat gccgaccgtg
    11761 aagacccacg tcggcagcct cctggccaag ctgcacgccc gggaccgcgc acagctggtc
    11821 atcatcgcgt acgagagcgg cctggccgag cggggaatgc cgacgtga
//
