BGC0001950 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001950 (Cylindrospermum moravicum CCALA 993)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	57,756	puwainaphycin F/minutissamide A	NRPS:Type I	100%

BGC0001950 - Region 1 - NRPS,T1PKS

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Location: 1 - 57,756 nt. (total: 57,756 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 57,757
Label		BGC0001950

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001950	1.88	NRP	puwainaphycin F, minutissamide A	Cylindrospermum moravicum CCALA 993
BGC0001951	1.52	NRP	puwainaphycin F, minutissamide A	Cylindrospermum alatosporum CCALA 994
BGC0001125	1.12	NRP, Polyketide	puwainaphycin A, puwainaphycin B, puwainaphycin C, puwainaphycin D	Cylindrospermum alatosporum CCALA 988
BGC0002071	0.77	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001844	0.72	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0002135	0.71	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001131	0.70	NRP	ambactin	Xenorhabdus miraniensis
BGC0001758	0.70	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833	0.69	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0002437	0.68	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001950	1.00	NRP	puwainaphycin F, minutissamide A	Cylindrospermum moravicum CCALA 993
BGC0001951	0.93	NRP	puwainaphycin F, minutissamide A	Cylindrospermum alatosporum CCALA 994
BGC0001125	0.82	NRP, Polyketide	puwainaphycin A, puwainaphycin B, puwainaphycin C, puwainaphycin D	Cylindrospermum alatosporum CCALA 988
BGC0001844	0.69	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0001758	0.69	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002135	0.68	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001131	0.68	NRP	ambactin	Xenorhabdus miraniensis
BGC0002071	0.68	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002518	0.67	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001833	0.67	NRP	icosalide A, icosalide B	Burkholderia gladioli

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 57756:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Thr) + (Thr - Asn - Thr - Asn - D-Ala) + (mal - Val) + (Me-redmal) + (NMe-Asn - Pro)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

puwA

Search NORINE for peptide: strict or relaxed

AMP-binding (513..915): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DATKVGEVGK
Nearest Stachelhaus code(s):: Asn DATKVGEVGK (100% 8Å match); OH-Asn or Asn DATKVGEVGK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1996..2401): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFMAQVVK
Nearest Stachelhaus code(s):: Pro DVQFMAQVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

puwB

PKS_AT (596..894): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 95.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.0
inactive: 160.9
Methoxymalonyl-CoA: 68.2
Methylmalonyl-CoA: 59.6
2-Methylbutyryl-CoA: 34.6
Propionyl-CoA: 31.7
Benzoyl-CoA: 30.8
Isobutyryl-CoA: 30.6
Ethylmalonyl-CoA: 30.3
Acetyl-CoA: 28.1
CHC-CoA: 14.1
trans-1,2-CPDA: 12.9
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

puwc

CAL_domain (53..497): pk

minowa: NH2

Prediction, score:

NH2: 198.4
AHBA: 10.2
fatty_acid: 9.2
Acetyl-CoA: 2.7
shikimic_acid: 0.0

puwE

Search NORINE for peptide: strict or relaxed

PKS_AT (610..906): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 198.1
inactive: 173.7
Methylmalonyl-CoA: 79.9
Methoxymalonyl-CoA: 79.0
Propionyl-CoA: 63.0
2-Methylbutyryl-CoA: 56.3
Isobutyryl-CoA: 44.1
Acetyl-CoA: 38.6
Ethylmalonyl-CoA: 37.8
Benzoyl-CoA: 21.9
fatty_acid: 21.6
CHC-CoA: 20.0
trans-1,2-CPDA: 17.0
3-Methylbutyryl-CoA: 14.2

AMP-binding (2523..2921): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWLGGTFK
Nearest Stachelhaus code(s):: Val DAFWLGGTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

puwF-G

Search NORINE for peptide: strict or relaxed

AMP-binding (540..948): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNVGMVHK
Nearest Stachelhaus code(s):: Thr DFWNVGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1610..2011): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DATKVGEVGK
Nearest Stachelhaus code(s):: Asn DATKVGEVGK (100% 8Å match); OH-Asn or Asn DATKVGEVGK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2668..3076): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNVGMVHK
Nearest Stachelhaus code(s):: Thr DFWNVGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3739..4140): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DATKVGEVGK
Nearest Stachelhaus code(s):: Asn DATKVGEVGK (100% 8Å match); OH-Asn or Asn DATKVGEVGK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4801..5194): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (100% 8Å match); Gly DLFNNALTYK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

puwH

Search NORINE for peptide: strict or relaxed

AMP-binding (457..865): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)