BGC0001966 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001966 (Phoma sp)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T1PKS	1	84,810	High	BII-rafflesfungin	NRPS:Type I+PKS:Iterative type I

BGC0001966 - Region 1 - NRPS,T1PKS

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Location: 1 - 84,810 nt. (total: 84,810 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 84,811
Label		BGC0001966

Tailoring functions

Oxidoreductases: oxidase

QCC62995.1: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

Transferases: glycosyltransferase

QCC62991.1: glycosyltransferase

smcogs: SMCOG1102: glycosyltransferase

Ligases: ligase synthase

QCC62990.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

QCC62999.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

QCC63000.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001966.3	0.97	NRPS, PKS	BII-rafflesfungin	Phoma sp.
BGC0002191.2	0.89	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001275.3	0.64	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002192.3	0.62	PKS	FR901512	fungal sp. No.14919
BGC0001254.3	0.59	PKS	ACT-Toxin II	Alternaria alternata
BGC0001160.5	0.57	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001268.4	0.57	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0002155.3	0.54	PKS	nectriapyrone C, nectriapyrone D, nectriapyrone	Pyricularia oryzae 70-15
BGC0002240.3	0.46	PKS	BAB, BAA	Metarhizium anisopliae
BGC0001606.3	0.45	PKS	gibepyrone-A	Fusarium fujikuroi IMI 58289

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001966.3	0.98	NRPS, PKS	BII-rafflesfungin	Phoma sp.
BGC0002191.2	0.75	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002135.2	0.72	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.72	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001166.5	0.71	NRPS	HC-toxin	Alternaria jesenskae
BGC0001135.5	0.71	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001825.4	0.71	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001833.4	0.71	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001261.3	0.71	NRPS	AM-toxin	Alternaria alternata
BGC0001240.4	0.70	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 22560 - 84810:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ala - Glu - Asn - Ser - Ser - D-Ser - D-Thr - Gly) + (Me-pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 56705 - 84810:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ala - Glu - Asn - Ser - Ser - D-Ser - D-Thr - Gly) + (Me-pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

QCC62990.1: X

AMP-binding (57..468): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: IYLYVGGGIK
Nearest Stachelhaus code(s):: Ile DYLCVGVVIK (26% 8Å match)
Stachelhaus code match:: 60% (weak)

QCC62999.1: Ala - Glu - Asn - Ser - Ser - Ser - Thr - Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (1024..1406): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVATITAIWK
Nearest Stachelhaus code(s):: Ala DVATITAIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2113..2502): Glu

nrpys: Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DVTHSGSVTK
Nearest Stachelhaus code(s):: Glu DVTHSGSVTK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3209..3607): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVSNVGSIFK
Nearest Stachelhaus code(s):: Asn DVSNVGSIFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4329..4695): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVQTVMAIHK
Nearest Stachelhaus code(s):: Ser DVQTVMAIHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5397..5795): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVQTVMAIHK
Nearest Stachelhaus code(s):: Ser DVQTVMAIHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (6501..6899): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVQTVMAIHK
Nearest Stachelhaus code(s):: Ser DVQTVMAIHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (8036..8436): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAQTIMAIHK
Nearest Stachelhaus code(s):: Thr DAQTIMAIHK (100% 8Å match); Thr or Ser DAQTIMAIHK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (9594..9998): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVSNVIGIVK
Nearest Stachelhaus code(s):: Gly DVSNVIGIVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

QCC63000.1: pk

PKS_AT (643..960): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 58.3%; inactive: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 63.5
Methoxymalonyl-CoA: 61.0
Malonyl-CoA: 60.5
Isobutyryl-CoA: 48.8
Ethylmalonyl-CoA: 30.0
Benzoyl-CoA: 26.0
fatty_acid: 24.8
Propionyl-CoA: 20.6
trans-1,2-CPDA: 15.8
inactive: 11.1
3-Methylbutyryl-CoA: 10.6
CHC-CoA: 9.5
2-Methylbutyryl-CoA: 7.2
Acetyl-CoA: 4.9

Link to PARAS substrate predictor