BGC0001975 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001975 (Streptomyces atratus)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	arylpolyene,NRPS,aminocoumarin	1	69,292	atratumycin	NRPS:Type I	100%

BGC0001975 - Region 1 - NRPS,aminocoumarin,arylpolyene

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Location: 1 - 69,292 nt. (total: 69,292 nt) Show pHMM detection rules used

Region on contig edge.

arylpolyene: (APE_KS1 or APE_KS2)
NRPS: cds(Condensation and (AMP-binding or A-OX))
aminocoumarin: (novK or novJ or novI or novH or SpcDK_cou)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 69,293
Label		BGC0001975

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001975	1.82	NRP	atratumycin	Streptomyces atratus
BGC0001185	0.94	NRP	bacillibactin	Bacillus velezensis FZB42
BGC0000401	0.90	NRP	paenibactin	Paenibacillus elgii B69
BGC0002108	0.89	NRP	cinnapeptin	Streptomyces viridosporus ATCC 14672
BGC0002695	0.85	NRP	bacillibactin, bacillibactin E, bacillibactin F	Bacillus sp. WMMC1349
BGC0001758	0.81	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002069	0.75	Other (Aminocoumarin)	styrolide A, styrolide B	Pseudomonas fluorescens
BGC0001918	0.73	NRP, Polyketide	JBIR-06	Streptomyces sp.
BGC0000265	0.71	Polyketide	rhodomycin	Streptomyces purpurascens
BGC0000309	0.71	NRP	bacillibactin	Bacillus subtilis subsp. subtilis str. 168

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001975	0.99	NRP	atratumycin	Streptomyces atratus
BGC0001133	0.76	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002108	0.72	NRP	cinnapeptin	Streptomyces viridosporus ATCC 14672
BGC0002528	0.71	NRP	aminochelin, azotochelin, protochelin	Azotobacter vinelandii CA
BGC0001629	0.71	NRP	jessenipeptin	Pseudomonas sp. QS1027
BGC0001185	0.71	NRP	bacillibactin	Bacillus velezensis FZB42
BGC0000401	0.69	NRP	paenibactin	Paenibacillus elgii B69
BGC0002017	0.69	NRP	potensibactin	Nocardiopsis potens DSM 45234
BGC0002695	0.69	NRP	bacillibactin, bacillibactin E, bacillibactin F	Bacillus sp. WMMC1349
BGC0000359	0.69	NRP	fuscachelin A, fuscachelin B, fuscachelin C	Thermobifida fusca YX

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 69292:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Thr - Ser - Trp - D-Asn) + (X - Pro - D-Val) + (Gly - D-Leu - Tyr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 30948:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly - D-Leu - Tyr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 24 - 69292:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Thr - Ser - Trp - D-Asn) + (X - Pro - D-Val) + (Gly - D-Leu - Tyr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

QBG38764.1

PKS_AT (16..277): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; inactive: 54.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 105.7
Methylmalonyl-CoA: 57.8
Methoxymalonyl-CoA: 55.5
inactive: 39.6
Propionyl-CoA: 27.8
trans-1,2-CPDA: 19.3
fatty_acid: 16.6
2-Methylbutyryl-CoA: 16.5
CHC-CoA: 15.8
Ethylmalonyl-CoA: 14.2
Isobutyryl-CoA: 11.4
Benzoyl-CoA: 9.8
Acetyl-CoA: 8.2
3-Methylbutyryl-CoA: 0.0

QBG38782.1

Search NORINE for peptide: strict or relaxed

AMP-binding (460..861): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1533..1930): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DALLIGAVAK
Nearest Stachelhaus code(s):: Leu DALLIGAVAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3074..3472): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DASTVAAVCK
Nearest Stachelhaus code(s):: Tyr DASTVAAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

QBG38783.1

Search NORINE for peptide: strict or relaxed

AMP-binding (463..861): X

nrpys: Ph-Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWTVAAVCK
Nearest Stachelhaus code(s):: Ph-Ser DAWTVAAVCK (100% 8Å match); Phe DAWTVAAVCK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1522..1934): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQYAAHVVK
Nearest Stachelhaus code(s):: Pro DVQYAAHVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2599..3005): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAYWWGGTFK
Nearest Stachelhaus code(s):: Val DAYWWGGTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

QBG38784.1

Search NORINE for peptide: strict or relaxed

AMP-binding (502..920): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1582..1990): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHISLVDK
Nearest Stachelhaus code(s):: Ser DVWHISLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2659..3056): Trp

nrpys: Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVAMVGAVCK
Nearest Stachelhaus code(s):: Trp DVAMVGAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3761..4171): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGEVGK
Nearest Stachelhaus code(s):: Asn DLTKVGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)