LOCUS       BGC0001985             23369 bp    DNA     linear   BCT 28-SEP-2019
DEFINITION  Streptomyces candidus culture NRRL:3601 MFS transporter gene,
            complete cds; pyrazofurin biosynthetic gene cluster, complete
            sequence; and MFS transporter gene, complete cds.
ACCESSION   BGC0001985
VERSION     BGC0001985
KEYWORDS    .
SOURCE      Streptomyces candidus
  ORGANISM  Streptomyces candidus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 16:39:47
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 67513
            Orig. end    :: 90882
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..1431)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1063:argininosuccinate lyase/adenylosuccinate lyase
                     (Score: 125.2; E-value: 6e-38)"
                     /gene_kind="biosynthetic-additional"
                     /note="adenylosuccinate lyase"
                     /product="pyfT"
                     /protein_id="QER90992.1"
                     /transl_table=11
                     /translation="MTTKEIVPDVLAARYASSDLVAIWAPRNRVATERRLWIAVMTAQR
                     ELGVDIPEQVIADYAAVVDQVDLESIKQRERVTLHDVKARIEEFNALAGHEHAHKGMTS
                     RDLTENIEQLMVRDSLRLIRHRCVSVLARLAELATEHAATAMAGRTHNVAAQVLPLGKR
                     FATAANELLVAVERVDDLLNRYPLRGIKGPVGTLQDMLDLFDGDTGKVLALETRIAEHL
                     GFERRFTSVGQVYPRSLDYEVVSALVQVAGAPTSLAKTIRLMAGHELAGEGFAEGQTGS
                     SAMPHKMNPRNCERISGLMVVLRGYASMAGELAGDQWNEGDVSCSVVRRVALPNAFFAL
                     DGLLQTTLAVLDGFTAAPRVVEAELERYLPFLATTKMLVAAVRAGQGRETAHELIKEHA
                     VAAATALSEGGTTNTLLTNLGSDERFPLDQAALTELLADRSAFTGVAAEQITEICARVA
                     KLLTSAPEAATYTPEPLL"
     subregion       1..23369
                     /aStool="mibig"
                     /contig_edge="False"
                     /label="other"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..23369
                     /contig_edge="False"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     misc_feature    1..23330
                     /note="pyrazofurin biosynthetic gene cluster"
     CDS             1764..2426
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115:HAD-superfamily hydrolase, subfamily IA, variant
                     (Score: 156.1; E-value: 1.6e-47)"
                     /gene_kind="biosynthetic-additional"
                     /note="HAD family hydrolase"
                     /product="pyfS"
                     /protein_id="QER90993.1"
                     /transl_table=11
                     /translation="MVFDLDGTLVDTPAAIGATLVEVLAGEGFTVDHAAVAATIGKPLV
                     PSVAGLLELAADDQAVRTVVTRYQRLFDERVLGRGTELLYAGVADGLAALRGSGLSCAI
                     ATSKDLRVATALLGSSGIAAHFPVVITHDQVAQGKPHPEMGLCAAADLGVDAGACAYVG
                     DAVGDMEMAVAAGMTPIGVAYGVASAAELTEHGAVQVCADFAEVVKAVSALAGRRTN"
     CDS             2606..2962
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MannoseP_isomer"
                     /gene_kind="biosynthetic-additional"
                     /note="cupin-domain containing protein"
                     /product="pyfR"
                     /protein_id="QER90994.1"
                     /sec_met_domain="MannoseP_isomer (E-value: 1.4e-11,
                     bitscore: 34.9, seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFIDWEKMPRTRSPRAKDSRRIASGQNMSFVRIEIEPTAEFTGET
                     HWHMHEQWVVVLAGDVRMTVADEEVQLTTGDVLYIPIDAPHAPLAVGPAGATYLEIFAP
                     PRMDLLPESIVPAV"
     CDS             complement(3022..4026)
                     /codon_start=1
                     /note="beta-ribofuranosylaminobenzene 5'-phosphate
                     synthase"
                     /product="pyfQ"
                     /protein_id="QER90995.1"
                     /transl_table=11
                     /translation="MQGTAVRVSSPARLSFTLISLDGESRRRNGIASIGAERPNATVTA
                     TATPGRATTVVGAEEETHHALVDGLAKLQRIWDGPDVALDVQSSLPAHSGFGSKTTTLI
                     AAGYAYGLLSGQTPDIRWLSATLGRGRTSGASTGLAEHGGFLVDCGHLNPPDFAAEPTK
                     YLRPSHFATEVQPPTPVIKMDFPDWPILILQVNGRNIGGPEELEWFTTTMPIPPAESWR
                     TSHLVFMGLAPAVAERDYDTFCSVVNELTFTGYFKRAQIALQGQEMVDLLDEGRSQSSV
                     DAIAMSSHGPTCFAFCRDTEAAAAWAEGLRQRGVIRAHWFTTVRNSGLAATWL"
     CDS             complement(4102..5139)
                     /codon_start=1
                     /note="SAICAR synthetase"
                     /product="pyfP"
                     /protein_id="QER90996.1"
                     /transl_table=11
                     /translation="MLMLHAHGRKHVKRWSSKDLEVLVPPSDGRPGEGVFVFSDRYSVF
                     DFGIMPDEIPGKGAASCAMAVRSFELFEAAGIRTHFLEQVAEDAIRIQLLDVDTDRTKG
                     RTGPGRTIPLQVIYRLALPQESSVHRRAAAGTLDQSTVPPYRDAPWLSEPMVEFTTKYE
                     ETDRFISRSEAAEVGAVEPEILEVVADLTTTAARTLEQHCGSIGLALVDGKAEFGFDAD
                     RQPIMIDHAGTPDENRFYLDGIPVCKELLRFLHPGLREVVQGYVAEGRPRAQWPLPKPL
                     APEVVAAVAEIYAALTAAWTAAPPSAAGTQRLARAVENFIAVCGISSELGQRTGLAAVP
                     SISAR"
     CDS             complement(5287..6909)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222:dehydrogenase (Score: 32.5; E-value: 7.9e-10)"
                     /gene_kind="biosynthetic-additional"
                     /note="succinate dehydrogenase/fumarate reductase"
                     /product="pyfO"
                     /protein_id="QER90997.1"
                     /sec_met_domain="DAO (E-value: 4.5e-22, bitscore: 69.5,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLEKFDVVVVGSGGAGLLAAARAAAAGLSVVVLERSDVLGGTTA
                     VSGGMLWVPNSSVMKQAGLPDSAEDALRYLERVVEGTVPRERLEHYVRTAPEMVDWLLA
                     HTGARFFPIDRPDYHSTWPGAVDVGRCLDNEPFPTASRPGLLERVRRGPQFPPLTYDER
                     HRARFTGPDASLLAQRLADGVLTVGAALVAALVAACDDLGVRFVLRARATSLLRDDNDA
                     AAVTGVRCEDGRRFSARAVVLANGGFEWDPASGAFLGDIPVVPVSPPVNTGDGLRMAMS
                     AGAAVERMNQAWWVPALQGVPEEYDGAPLTRHLVGERCLPGSIMVDRHGRRFVNEAVNY
                     HDITRVLFNFDADLHQPAHLPVWLVFDEKFRTSYNVGPAAPRDPVPSWLTSAATPAALA
                     EAIGVDPAELTKTVDRFSAHARAGVDPDFHRGESSHDRYYGDPRNPGNPCLGELVTPPF
                     HAVRVVPGALGSKGGPVTSTSGEVLTATGTAVPGLYACGNISAGVFGPGYPGSGGTLGP
                     ALTAGYAVGGALVERCGGAFATD"
     CDS             7433..8365
                     /codon_start=1
                     /note="LLM class flavin-dependent oxidoreductase"
                     /product="pyfN"
                     /protein_id="QER90998.1"
                     /transl_table=11
                     /translation="MDLTGPSSELVATARTVEEAGADYLVLPDRTAARPDGASPPAALI
                     AAAFLAAHTSEIGIVVTAATGYHEPYSLARQIASLDHISAGRVGWFADSATDAASDANH
                     RREGHDPAAAPETRALEFVPLVRDLWDSWEDDAFVHEKDTGRFVDPAKIHVVDHVGTAL
                     AVRGPLNVIRPPQGHPVVFARAADTSVAHHADVLVSDVPQQGHILAVEPFVAADEVAAR
                     ELHAAAGSPAGGDVLVGTPEQVAAQLRDRHVLVRFTTRAQLVAFTTHVLPLLDKSAPTG
                     ATLRERLGLPAVANRFAPTQDKELIGNAR"
     CDS             8355..9689
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083:oxidoreductase (Score: 113.2; E-value: 2.5e-34)"
                     /gene_kind="biosynthetic-additional"
                     /note="LLM class flavin-dependent oxidoreductase"
                     /product="pyfM"
                     /protein_id="QER90999.1"
                     /transl_table=11
                     /translation="MPADRELHLGLMFWATGTHQAGWRHPDASADAAYDIELIQEVCRT
                     AERAKFDFAFLGDRLACDPALQHSNPAQISRLEPFATAAAIAAATTHIGVVVTANPTYS
                     DPFGVARNLASLDHISGGRAAWNLVTGADAAAALNYSRDQHWDTQKRYDWAEETLEIAR
                     ALWDSGDQPINHRSEYFSIDGPLGLPRPPQGHVVLLNAGTSDQARELGARQADMVFAGP
                     QPTLAKRKEYYADIKARAAKYGREDHVTVMPGLTPIVAPTTEEAVALHDQLNSLIVLDP
                     EVEVGEVVRAGGIGEGFKRNLVSASEAFGVNLRGNDLNAVVPAETLARVSEDGRRRLAE
                     ITRLTRRTADGPERITYADLVHATPQQLSHVVVGNPEEIADEIQLWLEERMADGFNIYP
                     AYVPGSVTAFTELVVPVLQRRGLFRKDYRGRTLRDHLGLAVPAVR"
     CDS             9845..10621
                     /codon_start=1
                     /note="Streptomycin biosynthetic protein"
                     /product="pyfL"
                     /protein_id="QER91000.1"
                     /transl_table=11
                     /translation="MPISLLGEADSPRQAGVNAEHVRVLTGVENLPPILVHRHTMRVID
                     GMHRVQAALARGEDTIEVVFFDGDEAAAFVLAVELNGGHGLPLTLADRRTAAARIVRAY
                     PEWSDRRIAAAAGISPKTAGAVRAQLSSEEIPQMRERLGLDGRVRRVAPRTVPAHTTEP
                     ERVQARPARAKQPQPERQLDFGAVIYALRRDPSLRFNENGRTLLRMLDMHHLPAAGWSG
                     IIGAVPAHCASVVAELARECAEAWLVLADELDEKSD"
     CDS             complement(10667..11722)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103:FAD dependent oxidoreductase (Score: 162;
                     E-value: 4e-49)"
                     /gene_kind="biosynthetic-additional"
                     /note="fructosyl-amino acid oxidase"
                     /product="pyfK"
                     /protein_id="QER91001.1"
                     /sec_met_domain="DAO (E-value: 7.7e-40, bitscore: 127.9,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVLVIGAGAIGACAALRLAEAGAQVTVMSRDEPLDTLSAHSFGW
                     ANAIDEKNSAYFELSIEALAAQERLAADVAGPRQWLFRQGNLHWGRDSASAAAQRAIAD
                     GYRELGYPVEELTPEEVIRDLEPGLALENVTGPVYFYPRDMHVLGDVLLPAVLDRARAA
                     GADVRIGEQVESLDAVRADAVLCCAGRGNATLLPGLPLLPPGAPERLTRGLLVRTTPVS
                     APPNRIIHAPGLSIRPHTGNRLVLHCHDLDHTLTESSDVAALAEMALARLPEVLPGAAD
                     AEVEKAFVSVRPMPRDGMSIVGAVPGRNGVYVIATHSGLTLAPLLGEIAAREVLGDPHP
                     LAEPFQLTRFA"
     CDS             11883..13124
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1063:argininosuccinate lyase/adenylosuccinate lyase
                     (Score: 80.1; E-value: 3e-24)"
                     /gene_kind="biosynthetic-additional"
                     /note="phosphoribosylglycinamide synthetase"
                     /product="pyfJ"
                     /protein_id="QER91002.1"
                     /sec_met_domain="ATP-grasp (E-value: 1.4e-07, bitscore:
                     21.7, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSRSVLFVNLRRIKREGFESLVAARRLGYRVVLLGRALPEFAQP
                     LVDEFHQVDTYDTDLALKTAREIAAANDLAGVVNFTEIDVQLVAAIAAELGLPGMPPEA
                     AVLARNKLAMKQALAGIDGVLPKFARVSDLDDLCAAVADIGFPCVVKPTGASGSKGIFE
                     LHGESDLEPAMAELQRIADPSFDAVFRQFGAEFIVEEYLTGDELSVEGFVADGTVHQLL
                     LTDKVTTVPFHLEVSHRLPSVLPAAAQDQILACSEKIVRALGFDNCAFHLEAKWDGERM
                     RFIEVAARPAGDYIASHLVKAATGIDFFANVIRVATGEPLQLVPDRDLQAGLRFVFAES
                     AGTFDGLDGVTELFEEPGYDHVFTEVPIGAQVALPPANFGSQRVAAVCARAHDRAGLDA
                     LLDTAGEAIKVRIG"
     CDS             13156..14358
                     /codon_start=1
                     /note="saccharopine dehydrogenase"
                     /product="pyfI"
                     /protein_id="QER91003.1"
                     /transl_table=11
                     /translation="MRLTLLGCGRMGRGAGYALARDQRTTSITVVDHDRERAEELARWL
                     SGYAACPVKTGDSDAIAGSDAVAAALPWSGTRSVIELAAAAGVPVASITRPPGDESSDV
                     DAMANAAGVPVLLPVGLEPGLTELAAADVARRLTALTGGVRTLEVFCGGVPATPRAPWG
                     YTAFFGGELANHLPIAQRSSIAVEHGEIVDHPRFSGVEERHVAGVGLLEAYHDGMVPWL
                     ADHPALRDADCTQKTLRWPGFADAVTGLANLGLLDESPVAVDGVAVAPKRLVENVLSPR
                     LRAQPDDEDVVVLDVSATGLPDADGSTLSIRSLLVDRADRETGLAAMTRTTGFTLAAAV
                     TLLADRTVGGAGWLRPHLALSERHFARMKTDLAALGVRWTADGEALHGHRAGTVASKGT
                     D"
     CDS             14358..15671
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1080:lysine/ornithine N-monooxygenase (Score: 386.4;
                     E-value: 2.4e-117)"
                     /gene_kind="biosynthetic-additional"
                     /note="L-lysine 6-monooxygenase"
                     /product="pyfH"
                     /protein_id="QER91004.1"
                     /transl_table=11
                     /translation="MSTGHDVLDVVGIGIGPANLSLAALLAAESSELNVAFLDRKPHFG
                     WHSGLMLPDAQMQVHYLKDLVTPVDPTNPFSFTAFLVATKRFYRLLVTGRSKVPRREFE
                     QYCRWAAERIPNLRFGVEVREVTWNGELFVVHTDQGVLHARNVVSGTGLVPRLPSFATG
                     HDPQEVFHASTLLDVPRTFAGRRVAVVGGGQSGAEVVHHLLTSPDRPASIVWGTSRSNL
                     LPLDDSPFVDELFVPNYSRYFHGLPAKRRMELVDEQKMASDGVSVSLLEAIYHRLYDLE
                     MLEGEERPCRMLLGHSLTTVDKTPTGLLTRWMPGAGAEVGHEVDVVICATGYRHGLPRP
                     LHGLPRHLREAMCGDDGEITVRPDFSLDWEGPQDRRMYVQNAARHSFGVADPNLSLLAW
                     RSATIANSVLGYERYDVGEVGAVLDWTSSADSDVALTF"
     CDS             15690..17696
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MannoseP_isomer"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MetRS-like"
                     /gene_kind="biosynthetic-additional"
                     /note="Methionine-tRNA ligase"
                     /product="pyfG"
                     /protein_id="QER91005.1"
                     /sec_met_domain="MannoseP_isomer (E-value: 7.6e-12,
                     bitscore: 35.8, seeds: 17, tool: rule-based-clusters)"
                     /sec_met_domain="MetRS-like (E-value: 1.7e-136, bitscore:
                     445.5, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVSEIPRELADLARADFVRSAAPAWAEAAGVPFNVRRVTLRPGE
                     TTAEHNHHDLEVWVMLDGVGEVGWDGHQRVLTAGDSVYLPPLAPHTLRNLSSDRPLSFF
                     SMWWENLSALAAVHAERREQAVERQGRPVLLLPSFPTPNGELHLGHLSGPFLNADACRR
                     ALLAAGERAHLLLGTVGHQSQVSAAAEAEGLSFHELAERNTDAIIEGLQAAGIDWDVFV
                     RPSEPAYPAMATSVFESLRDRGVLVRRTEPTNYCEPCGRFLLEAFVAGHCPHCGSNQTA
                     GIECELCALPYDDRDLVDPSCATCGAAATQRPLTRYFMPLEPLRDELSGYLRGAAMHGR
                     LRAYTERVLAKTLPDLPVSIPAEHGIPIHVEDASGPAEQRMYSAFELAARFLTALDGFA
                     DGWEAYARQENPRTVLFFGFDNAFLRAFAFPAVLGAFTDALPLPEALVCNDFYLLDGEK
                     FSTGRKHAVWARQAVTPANADQLRLYLAATSPDVRRRDFTTRGYAEFVTAELIGRWQRR
                     LDDVGGRVAEHFGGLTPEAGGWHAEAERFYGQIKEFASCATLDYLPGRFKPRAVVAAAC
                     AFIRQAEDFAEVSADATPGSGIARTCAALELMALRTLAMAVWPLAPEFGRRVAAALGED
                     TIALEPTPRWVRPDTEIKFATDHFSPDEVVAGR"
     CDS             17740..18492
                     /codon_start=1
                     /note="SAICAR synthetase"
                     /product="pyfF"
                     /protein_id="QER91006.1"
                     /transl_table=11
                     /translation="MRDTDTDTDTDTDADVAGPASVVDEREPDITGRSKKLWLVPGDRC
                     VVELVPSLRSFTRARDELVDETGPLRLDFYEKAAARLSDAGVECAFRARLGPVTYLADY
                     RPAPPFEVIVKNVATGSTTRKYPGLFPDGEPLPRPVVKFDYRVDPEDQPIGEDYLRVLD
                     LPVDLMREQALLVNDVLRDWLNPLRLLDFCVIFGFSSAGEISLISEVSQDCMRLRHPDG
                     SPLDKDLFRGGASAGELVEQWKRAFDGL"
     CDS             18482..19183
                     /NRPS_PKS="Domain: PKS_KR (6-160). E-value: 1.2e-09. Score:
                     30.2. Matches aSDomain: nrpspksdomains_QER91007.1_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     133.5; E-value: 1.5e-40)"
                     /gene_kind="biosynthetic-additional"
                     /note="short-chain dehydrogenase"
                     /product="pyfE"
                     /protein_id="QER91007.1"
                     /transl_table=11
                     /translation="MGSESPVALVTGSNRGSGRAIAARLHERGYRVFSLNRTVTGEDWL
                     GERECDLASRESLAAGVAAVLERAGRLDVVIANAVERVLDPIEKMSEQDWDRQLEINLS
                     SVFRLVKQVLPALRRSCGLFLVMGSHAGSRYFEGGSAYSATKAALKALVETLLLEERNN
                     GVRACLLAPGAISNLDGDDAPTKVSVESVGTCVASIVADWPADLVVGELEFRPALLPES
                     PVTGIDRLLHV"
     aSDomain        18500..18961
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_QER91007.1_PKS_KR.1"
                     /evalue="1.20E-09"
                     /label="QER91007.1_PKS_KR.1"
                     /locus_tag="QER91007.1"
                     /protein_end="160"
                     /protein_start="6"
                     /score="30.2"
                     /tool="antismash"
                     /translation="VALVTGSNRGSGRAIAARLHERGYRVFSLNRTVTGEDWLGERECD
                     LASRESLAAGVAAVLERAGRLDVVIANAVERVLDPIEKMSEQDWDRQLEINLSSVFRLV
                     KQVLPALRRSCGLFLVMGSHAGSRYFEGGSAYSATKAALKALVETLLLEE"
     CDS             19183..19965
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1179:hydrolase (Score: 126.8; E-value: 1.8e-38)"
                     /gene_kind="biosynthetic-additional"
                     /note="HAD family phosphatase"
                     /product="pyfD"
                     /protein_id="QER91008.1"
                     /transl_table=11
                     /translation="MFFVVTDLDGTLLTSDRLVTERAKSALTKVRSSGGVVVLITARPL
                     RDVTEIGQEVGANFLVCSGGAVLYDPVRGELVRATTLGSRRATSLISLLRQTFPGIRLG
                     VDHLARCDLDPGFHVGAPGVADRHATEPLRQVAEPAVKLIAQSDEMPVDRLARAISALV
                     GAACSVAVPCSYFVDVLPAGVHKAVGLGELHECHGRVLPSIAFGDMPSDLPMLRWADVS
                     VATANAHPAVLEACDHVTAHHDADGVAVYLESLISTGG"
     CDS             19982..20836
                     /codon_start=1
                     /note="ATP phosphoribosyltransferase"
                     /product="pyfC"
                     /protein_id="QER91009.1"
                     /transl_table=11
                     /translation="MLPTGSLHEPTLRLFDAAGLTVHRQASRALRAHVDFPGIERVVFG
                     KPREIPGLVADGVVDLGLTGTDWIEESGAKVEVVHEFQYSKTTSAGWRVVLAVPVDHPA
                     RTAADLPAGVRVASEYPAIARRYFEEELGQEARIVHSHGSTEAKVPDLADAVLEIVETG
                     DTLRHNDLRELVVVRRCAVQLVVATQADPVVRATAEKVALLLKGARAATEHALLTVVAP
                     ADCWDRVRQELPRYWWLLGGDPETVVAQATYELRGVAEAITRLTQAGAVQVVETPMRKL
                     VTA"
     CDS             20918..22141
                     /codon_start=1
                     /note="phthalate 4,5-dioxygenase"
                     /product="pyfB"
                     /protein_id="QER91010.1"
                     /transl_table=11
                     /translation="MATKSMNETLTRVGRGTPMGELLREYWMPVMRSERVVAGGEPIAV
                     ELLGDRYVIFRGDDGTVACFDEACPHRGASLALARNEDCALRCIYHGWKFTTDGRTVET
                     PSEPEDGGRFASKVKLNHHPVVDAGGVIWVWAGGTGREPSPFPRFAFTDLPSSHVFGIV
                     AILDCNWVQGLEADIDSAHVSLLHETEAAKGPLKDLLDDRAPRDEIEKTSWGIRYAAIR
                     RLSTGDSLVRVKPMVMPWYTIVPELPNGDRLWHAWVPINDHQTIFWYLWYNEDQPVDPN
                     YFADQFGLDLENMNKDNFREGYSRKNMWGQDRAAMRAGTSFSGISGLAAQDIAVQESMG
                     AIVDRSIENPGKSDTGLVRARHFLLDAIKDKAEGRVPAGLGDEVDYRKISSANVVVSDG
                     DDWRQLVV"
     CDS             complement(22167..23330)
                     /codon_start=1
                     /note="amidohydrolase"
                     /product="pyfA"
                     /protein_id="QER91011.1"
                     /transl_table=11
                     /translation="MATLVLTGAATVFSGDLAVPLLDADTVVCRDGMIHATGKASSLTH
                     EIDGADEVIDLRGGTIAPGLIDSHGHVTFGDYSPRQRAVDYLAGYVQGGITTTVSAGEV
                     HVPGRPRDRAGVKALAVAAQRCFSEYRPGGMRVRAGAVLIEPTLSEEDFRELAATGVRL
                     AKYGFGAFTDPVDGVEHVRWAREAGLTVMCHAGGASAAGGASLGGEALLALRPDVCGHA
                     NGGPTALPFAEVEALFAETDMALQVVQAGNLKAALRIVEHAASRDELHRVVVGSDTPSG
                     FGVMPLAVLKTVLELTALGGLEPEVAWSLATGTVADVWHLPEGRVRRGAPADLLGLAAP
                     AGSQADTVAEAMRVGDIPAITVVVTAGTVRAMPGRMTPRPAVAAELR"
ORIGIN
        1 tcacaacaac ggttccggag tgtaggtggc cgcctcgggg gcgctggtga gcagcttggc
       61 gacccgtgcg cagatctcgg tgatctgctc ggccgctacc ccggtgaacg ccgaccgatc
      121 ggccagcagc tcggtcaagg ccgcctggtc caacgggaac cgctcgtccg aaccgaggtt
      181 ggtcaggagc gtgttggtgg tgccgccctc gctcagggcg gtggccgccg ccaccgcgtg
      241 ttccttgatc agctcgtgcg ccgtctccct gccctgcccg gcccgcaccg ccgcgaccag
      301 catcttcgtg gtggccagga acggcaggta gcgctccagt tcggcttcga ctacccgtgg
      361 ggccgcggtg aagccgtcga gcaccgccaa ggtcgtctgg agaagtccgt ccagggcgaa
      421 gaacgcgttc ggcagcgcga ccctgcgcac caccgaacag gacacgtcgc cctcgttcca
      481 ttggtcaccg gcgagctcgc cggccatcga ggcgtaaccg cgcagtacca ccatcagacc
      541 gctgatccgc tcgcagttgc gcggattcat cttgtgcggc atggccgacg agccggtctg
      601 cccctccgcg aacccctcac cggccagctc gtgcccggcc atcagccgga tcgtcttcgc
      661 caggctggtc ggggctcctg ccacctgcac gagcgcggac acgacctcgt agtccagcga
      721 tcgggggtag acctggccga cgctcgtgaa acggcgttcg aagccgaggt gctcggcgat
      781 ccgcgtctcc agggcgagga ccttcccggt gtcgccgtcg aacaggtcca gcatgtcctg
      841 cagggtgccg accggtccct tgatgccgcg caacgggtat cggttcagca ggtcgtcgac
      901 gcgctcgacg gcgaccagca gctcgttggc cgcggtggcg aaacgcttgc ccagcgggag
      961 cacctgcgcg gccacgttgt gcgtgcggcc cgccatcgcg gtggcggcgt gctcggtggc
     1021 gagctcggcg agtcgggcca gcacgctgac gcaccggtgc ctgatcagcc gcaggctgtc
     1081 gcgcaccatc agctgctcga tgttctcggt gaggtcgcgc gaggtcatgc ccttgtgggc
     1141 gtgctcgtgc cccgcgaggg cgttgaactc ctcgatccgc gccttcacgt cgtgcagcgt
     1201 cacccgttcc cgctgtttga tcgactcgag atcgacctgg tccaccacgg cggcgtagtc
     1261 ggcgatcacc tgctcaggga tgtccacgcc cagctcgcgt tgggcggtca tcaccgcgat
     1321 ccacagccgg cgctcggtcg ccacccggtt gcgcggtgcc cagatggcca ccaggtcgga
     1381 tgaggcgtac ctggcagcaa ggacgtcggg cacgatctct ttggtggtca tgtcttcctc
     1441 ggtaggtggg accggtggtg gggctcggcg ccacccggag agtcctcagt ggaagtccgc
     1501 ggacggccgt gtgccggaga actcgtccgt ccccgcgtcc cgttgccccc tcggtgcacc
     1561 agcgagtcaa ccagcaggct cgcgccggag acaaccatcc gtccacagtg gaccgcacga
     1621 agcgggtgcg ggtagggttg tcgtcgtcgt gtcctgggtg cggtaacgtc gaaatctcgt
     1681 gtgtccgcgc gctgtgtggt tggattgtgg ccgaacgatt cacatcgacc tgaagtgagg
     1741 ctgcgggcgg catgcttgag gcagtggttt tcgatctcga cggcacactg gtcgacacgc
     1801 cggccgccat cggcgcgacg ctggtcgagg tgctcgccgg agaagggttc accgtcgacc
     1861 atgcggcggt ggccgcgacc atcggcaagc cgctcgtacc cagcgtggcc ggcctgcttg
     1921 agctcgctgc cgacgaccag gccgtgcgga ccgtggtcac caggtaccag cggctgttcg
     1981 acgagcgagt gctcggccgg ggcaccgagc tgctctacgc cggagtcgcc gacggccttg
     2041 ccgcactgcg cgggtccggg ctgtcctgtg ccatcgccac ctcgaaggac ctgcgagtgg
     2101 ccaccgctct gctgggcagt tccggtatcg cggcgcactt cccggtggtg atcacgcacg
     2161 accaagtcgc acaaggaaaa ccgcaccccg aaatgggtct gtgcgcagcc gccgacctcg
     2221 gcgtggacgc gggtgcgtgc gcgtacgtgg gagacgcggt gggggacatg gagatggccg
     2281 tcgcggccgg gatgaccccg atcggagtgg cctacggagt ggcgagcgcg gccgaactga
     2341 ccgagcacgg cgcggtacag gtctgcgcgg acttcgcgga ggtcgtcaaa gcggtgtcgg
     2401 ccctcgccgg gcggcgcacg aactgaccgc cgcgcccggg agacgacccc gtcgatggac
     2461 aaccgcgagt ggtgctggga ttctctgctc gactcgtcct gacagtccga gtcgatcgtc
     2521 ggctgtccgc cgtgtttttc gcgtgtccca aaggacaatc gcgtcgcgga gccgatcagc
     2581 gtccgaatcg atgggagttc aattcgtgtt catcgactgg gagaagatgc cgcgcacccg
     2641 gagcccacgt gcgaaggaca gccggcggat cgccagcggg cagaacatgt ccttcgtgcg
     2701 catcgagatc gagccgaccg cggagttcac cggcgagacc cactggcaca tgcacgagca
     2761 gtgggtggtc gtgctcgcgg gagatgtccg gatgaccgtc gccgacgagg aggtccagtt
     2821 gaccaccggt gacgtgctct acattcccat cgacgcaccg cacgccccgc tggcggtcgg
     2881 tcccgcgggc gcgacctacc tggagatctt cgcgccgccg aggatggacc tgctgccgga
     2941 gagcatcgtt ccggcggtct gacgacgaaa cctgtgtgcc cccggcacga tccgatcgtc
     3001 cgggggcaca caggttttct gctacagcca ggtcgcggcc aagccgctgt tgcggaccgt
     3061 ggtgaaccag tgggcccgga tcacgccccg ctgccgcagt ccctcggccc aggcggccgc
     3121 ggcctcggtg tcccggcaga acgcgaaaca ggtcggtccg tggctgctca tcgcgatggc
     3181 gtccacactg gactgactgc ggccctcgtc cagcagatcg accatctcct gaccctgcaa
     3241 ggcgatctgc gcgcgcttga agtagccggt gaaggtcagc tcgttgacca cggagcagaa
     3301 cgtgtcgtag tcgcgctcgg ccacggcggg cgcgagcccc atgaacacca ggtgcgaggt
     3361 gcgccacgac tcggccgggg ggatcggcat ggtggtcgtg aaccactcga gttcctcggg
     3421 accgccgatg ttccggccgt tcacctgcag gatgaggatc ggccagtccg ggaagtccat
     3481 cttgatcacc ggagtgggcg gctggacctc ggtcgcgaaa tggctggggc gcaggtactt
     3541 cgtcggctcc gcggcgaagt cgggcggatt gaggtggccg cagtcgacca ggaaccctcc
     3601 gtgttcggcc agcccggtgc tggcaccgga ggtgcggccg cggccgagag tggcggagag
     3661 ccagcggatg tccggggtct ggccgctcag cagcccgtag gcgtagccgg ccgcgatcag
     3721 ggtggtggtc ttggagccga acccgctgtg cgcgggcagc gaggactgga cgtccagggc
     3781 gacgtccggg ccgtcccaga tccgctggag cttggccagt ccgtcgacca gcgcgtggtg
     3841 cgtctcctcc tcggccccga cgacggtggt ggcgcggccg ggggtcgcgg tggcggtgac
     3901 cgtcgcgttc ggtcgctccg ccccgatgga ggcgatcccg ttgcggcggc gggattcgcc
     3961 gtcgaggctg atcagggtga agctgagccg ggcgggggag gacacccgca ccgcggttcc
     4021 ctgcaccgtg ctcgcgtcgt tggtagtcgt cgatgctgtc aaggccgtgc tcccgtcgtg
     4081 gctccggtcg ttggcgagtg gtcagcgggc actgatcgac ggcacggcgg ccaggccggt
     4141 gcgctgcccg agttccgagg agatcccgca gacggcgatg aagttctcca ccgcgcgtgc
     4201 gagccgctgc gtcccagctg ccgagggcgg ggcggcggtc cacgcggccg tcagcgcggc
     4261 gtagatctcg gcgacggcgg cgacgacctc gggggccagc ggcttcggca gcggccactg
     4321 cgctcgcggt cgtccctcgg cgacgtagcc ctgcacgacc tcgcgcaggc cggggtgcag
     4381 gaagcgcagc agttccttgc agaccgggat cccgtccagg tagaagcggt tctcgtccgg
     4441 ggtgcccgcg tggtcgatca tgatcggctg gcggtcggcg tcgaacccga actccgcctt
     4501 gccgtccacc agcgccagac cgatgctgcc gcagtgctgt tccagcgtgc gcgcggcggt
     4561 cgtggtcagg tcggccacca cctcgaggat ctcaggctcg accgcgccga cctcggctgc
     4621 ctccgaccgg ctgatgaacc ggtcggtctc ctcgtacttg gtggtgaact cgaccatcgg
     4681 ctcggacagc cacggcgcgt cgcggtaagg gggcacggtg ctctggtcga gggtgcctgc
     4741 ggcggcgcgg cggtgcacag aggactcctg aggcagcgcg aggcggtaga tcacctggag
     4801 cgggatggtc ctgcccggcc cggttcgtcc cttggtgcgg tcggtgtcca cgtcgagcaa
     4861 ctggatccgg atggcgtcct ccgccacctg ctccaggaag tgcgtgcgga tgccggcggc
     4921 ctcgaacagc tcgaacgacc gcaccgccat cgcgcaggaa gcggcgccct tgcctgggat
     4981 ctcgtcgggc atgatgccga agtcgaacac ggagtagcgg tcggagaaga cgaagacacc
     5041 ctcacccggc cggccgtcgc tcggcgggac cagtacttca agatccttgc ttgaccagcg
     5101 tttcacgtgt ttccttccgt gggcgtgcag catcaacacg acgccgatcc cggcgtcgcg
     5161 cgcgatggac gatctctttc tcccccaggg gaagccccgg cgcgggaacc tatacgcgag
     5221 ctggttcgct gccaagacaa gccattcgtg ccgggcacgc cgtgcgccgt caagtgagga
     5281 aattcctcag tcggtggcga acgccccacc gcagcgctcg accagcgccc cgcccaccgc
     5341 gtagccggcg gtcagcgcgg gacccagcgt gccgcccgat cccgggtagc cgggcccgaa
     5401 cacgcccgcg gagatgttgc cgcacgcgta cagacccgga accgccgtcc cggtcgccgt
     5461 gagcacctca ccggacgtgc tggtgaccgg gccgcccttg gagccgagcg cgcccggcac
     5521 gacccgcacg gcgtggaacg gcggggtgac gagttcaccg aggcacggat tgccggggtt
     5581 gcgcgggtcg ccgtagtagc ggtcgtgcga gctctcgccg cggtggaagt ccgggtcgac
     5641 gccggcacgg gcgtgggcgg agaagcggtc gacggtcttc gtgagctccg ccgggtcgac
     5701 gccgatcgcc tcggccagcg cggcgggtgt ggcggcactg gtgagccagg agggaaccgg
     5761 atcgcgcggt gcggcgggac cgacgttgta gctggtgcgg aacttctcgt cgaacacgag
     5821 ccacaccggc aggtgggccg gctggtgcag gtcggcgtcg aagttgaaca ggaccctggt
     5881 gatgtcgtgg tagttcaccg cctcgttcac gaaccggcgg ccgtggcggt cgaccatgat
     5941 cgagcccggc aggcagcgct caccgaccag gtggcgggtc agcggcgcgc cgtcgtactc
     6001 ctcgggcacg ccctgcagtg cgggcaccca ccaggcctgg ttcatgcgtt ccaccgcggc
     6061 gccggcggac atcgccatgc gcagaccgtc gccggtgttc accggcggac tgaccgggac
     6121 gaccgggatg tcgccgagga aagccccgga ggccggatcc cattcgaagc cgccgttggc
     6181 cagaacgacg gctctggcgc tgaaccggcg gccgtcctca caccgcacgc ccgtcacggc
     6241 ggcggcgtcg ttgtcgtcgc gcagcaagga cgtggcacgc gcccgcagga cgaacctgac
     6301 accgaggtcg tcgcacgccg cgaccagcgc ggccaccagt gccgcgccca cggtcagcac
     6361 gccgtcagca agtcgctggg ccagcaggga agcgtccggg ccggtgaaac gggcacggtg
     6421 acgttcgtcg taggtcagcg gagggaactg cgggcccctg cgcacacgct cgagcaggcc
     6481 gggacgcgac gccgtgggga acggctcgtt gtcgaggcaa cggccgacgt cgacggcgcc
     6541 gggccaggtg gagtggtagt ccgggcggtc gatcgggaag aaccgggcgc cggtgtgcgc
     6601 cagcagccag tcgaccatct ccggcgcggt gcggacgtag tgctccaggc gctcgcgcgg
     6661 gaccgtgcct tccacgaccc gctcgaggta ccgcagcgcg tcctctgcgg agtcgggcag
     6721 accggcctgc ttcatcacgg agctgttcgg cacccacaac atgccgccgg acaccgcggt
     6781 ggtgccaccg agcacgtccg agcgttcgag cacgaccacg gacaggcctg cggccgccgc
     6841 cctggcggcg gcgagcaggc cggcgcctcc cgagcccacg acgacgacat cgaacttttc
     6901 caggtccacc caattacctt ccccgaaacc ggacgacggg gacagtattt gtggttgttg
     6961 tggatgggca agtgagagaa cagttttacg tctcactcgg acggagcatc ggaattcacg
     7021 ctgggtgacg accggccggg tgtcggaggg ggcctgttgt tgccgagtcg gcagtaatat
     7081 cggggtgttg ttccggcatg tggacgtgat gtggcgtcga agtggtcgta cgcgtgtgta
     7141 ctgcggcgat tggctcgatc gccgcctcgg ccggccgtga cggtggcctg cgtctggctg
     7201 ggacgttaaa tccgcgtacg cgaacgtcgg cgaccggcaa cttcttcacg gaatgattga
     7261 tttggttgcg aacgcgccac cgtgccggtt tactggcggt cggccgctgg ggagcggtgc
     7321 gaggggttcc agcgagctgt gtctcgaaac cagcaactgt gtggggggtt ggcgcatgcg
     7381 ctccggcgct tattcgtcat cagcaacgat cgtcggctat cacttgtccg gaatggacct
     7441 gacgggtccg tcctccgagc tcgtggcgac cgcgcgcacg gtggaagagg ccggtgccga
     7501 ctacctggtg ctgcctgacc gcacggcagc gaggccggac ggcgcgagcc ctccggccgc
     7561 gctgatcgcc gccgccttcc tggccgcgca caccagcgag atcgggatcg tcgtcaccgc
     7621 ggcgaccggc taccacgagc cgtacagcct ggcgcgtcag atcgcctcgc tggaccacat
     7681 cagcgccggt cgcgtcggct ggttcgccga cagcgccacc gatgccgcct ccgacgccaa
     7741 ccaccggcgt gagggccacg acccggcagc cgccccggag accagggcgc tcgagttcgt
     7801 gccgctggtc cgcgacctgt gggacagctg ggaggacgac gcgttcgtgc acgagaagga
     7861 caccggcagg ttcgtcgacc cggcgaagat ccacgtcgtc gaccacgtcg gcacggcgct
     7921 cgccgtgcgc gggccgttga acgtcatccg cccgccgcag gggcaccccg tcgtcttcgc
     7981 gcgggccgcc gacacctcgg tcgcgcacca cgccgacgtg ctggtttccg atgtcccgca
     8041 acagggtcac atcctcgcgg tcgagccctt cgtcgcggcg gacgaagtcg ccgcgcgcga
     8101 actccacgca gcggcgggaa gccccgccgg cggcgacgtc ctcgtcggta cccccgagca
     8161 ggtggccgcc cagctgcgag accgccacgt cctggtgcgg ttcacgactc gggcacagct
     8221 ggtcgcgttc acgacacacg tactgccgtt gctggacaag agcgctccca ccggggcgac
     8281 gctgcgcgag cggctcgggc tgcccgcagt ggccaaccga ttcgcgccga cccaggacaa
     8341 ggagctcatc ggcaatgccc gctgaccgcg aactgcacct cggcctgatg ttctgggcca
     8401 ccggcacgca ccaggccggc tggcggcacc cggacgccag cgccgacgcg gcctacgaca
     8461 tcgaactcat ccaagaggtg tgccgcacgg ccgagcgcgc caagttcgac ttcgcgttcc
     8521 tcggcgaccg gctcgcctgc gaccccgcgt tgcagcacag caacccggcg cagatctccc
     8581 ggctggagcc gttcgccacg gccgccgcga tcgccgccgc caccacgcac atcggggtcg
     8641 tcgtcacggc gaacccgacg tactcggacc cgttcggcgt ggccaggaac ctcgcctcgc
     8701 tggaccacat cagcgggggc cgcgcggcgt ggaacctggt caccggcgcg gacgccgcgg
     8761 cggcgttgaa ctacagccgc gaccagcact gggacaccca gaagcggtac gactgggccg
     8821 aggagacgct ggagatcgcc cgcgcgctgt gggactccgg cgaccagccg atcaaccacc
     8881 gcagcgagta cttctcgatc gacgggccgc tcggcctgcc ccggccgccg cagggccacg
     8941 tcgtcctgct caacgcgggc acctcggacc aggcgcggga actgggcgcg cggcaggccg
     9001 acatggtgtt cgcgggcccg cagccgacgc tggccaagcg caaggagtac tacgccgaca
     9061 tcaaggcccg cgcggcgaag tacggccgcg aggaccacgt cacggtgatg cccggcctca
     9121 ccccgatcgt cgcgcccacg accgaggaag ccgtggcgct gcacgaccag ctgaactcgc
     9181 tgatcgtgct cgaccccgag gtcgaggtcg gtgaggtcgt gcgcgcgggt gggatcggtg
     9241 agggcttcaa gcgcaacctg gtctcggcct cggaggcgtt cggcgtgaac ctgcggggca
     9301 acgacctgaa cgccgtggtg cccgcggaaa cgctggcacg cgtttccgag gacgggcgca
     9361 ggcggctcgc cgagatcacc cggctcaccc ggcgcaccgc cgacggcccg gagcgcatca
     9421 cctacgcaga cctcgtgcac gccacaccgc agcagctgtc gcacgtggtc gtcggcaacc
     9481 cggaggagat cgcggacgag atccagctct ggttagagga gcggatggcg gacggcttca
     9541 acatctaccc ggcctatgtg cccggctcgg tcaccgcgtt caccgaactc gtggtgcccg
     9601 tgctgcagcg gcgggggttg ttccgcaagg actaccgggg tcgcaccctg cgcgaccatc
     9661 tcggcttggc ggtacctgcg gtgcgttgac accgtgagaa gtagacgccg cgtcgtccat
     9721 ccaccacacg gccggttccg gccgtggttc gaccatgcgt tcactggggg aagagttgtc
     9781 tgtgcagttg cttcgtgagg aaaggacttc ctggtccatc cgggagaaat cgggttgtgt
     9841 caccgtgccg atctcgttgc tgggggaggc ggactctcct cggcaggcag gtgtcaacgc
     9901 ggagcacgtg cgggtgctca cgggggtgga gaacctgccg ccgatcctgg tgcaccgcca
     9961 cacgatgcgc gtcatcgacg gcatgcaccg cgtgcaggcc gcgctcgccc gcggggagga
    10021 caccatcgag gtggtgttct tcgacggcga cgaggccgcg gcgttcgtgc tcgccgtcga
    10081 gttgaacggc ggacacggcc tgccgctcac gctcgccgac cgcaggacgg ccgctgcacg
    10141 catcgtgcgg gcctatcccg agtggtcgga ccggcgcatc gccgcggcgg ccggcatctc
    10201 gcccaaaacc gccggcgcgg tgcgggcgca actgtcaagt gaggaaattc ctcagatgcg
    10261 cgaacggctg ggactcgacg ggcgagtgag gcgggtggca ccgcgtaccg tgccggctca
    10321 taccaccgag ccggagcggg tgcaggccag gccggcgcgg gcaaaacaac cgcagcctga
    10381 gaggcagctc gacttcggcg cggtcatcta cgcgctgcgg cgcgatccct cgttgcggtt
    10441 caacgagaac gggcggacgt tgctccggat gctggacatg caccacctcc ccgcggcggg
    10501 gtggagcggc atcatcggcg cggtgcccgc gcactgcgcg tccgtggtgg cggagctggc
    10561 gcgcgagtgc gccgaggcgt ggttggtcct cgcggacgaa ctcgacgaga agtcggacta
    10621 gtggtacgtc ttccggcgcc cctgccgggc gccggaagac gcggtttcag gcgaacctgg
    10681 tgagctggaa cggttcggcc agcgggtgcg ggtcaccgag cacctcgcgc gccgcgatct
    10741 cgccgagcag cggcgccagg gtcaggccgc tgtgcgtggc gatcacgtag acgccattcc
    10801 tgcccggcac cgcgccgacg atggacatcc cgtctcgcgg catcggccgc acgctcacga
    10861 acgccttctc cacctcggcg tccgccgcac cgggcaggac ctcgggcagc cgggcgagcg
    10921 ccatctccgc cagcgccgcc acatcgctcg actcggtgag cgtgtggtcg aggtcgtggc
    10981 agtgcagcac cagccggttg ccggtgtgcg gccggatgct cagccctggc gcgtggatga
    11041 tccggttggg cggcgcggac accggcgtgg tgcgcaccag caggcctcgg gtcagccgct
    11101 ccggggcgcc gggtggcaac agcggcagcc cgggcagcag agtggcgttg ccccggcccg
    11161 cgcagcacag caccgcgtcc gcgcgcaccg cgtccagtga ctcgacctgt tccccgatcc
    11221 gcacgtccgc cccggccgcc ctggccctgt ccagcaccgc gggcagcagc acgtcgccga
    11281 gcacgtgcat gtcgcgcggg tagaagtaga cgggtccggt gacgttctcc agcgccagtc
    11341 ccggttccag gtcgcgaatg acctcctcgg gcgtcagttc ctccaccgga tagccgagtt
    11401 cgcggtagcc gtccgcgatg gcccgctgcg cggccgcgct cgcgctgtcg cggccccagt
    11461 gcagattccc ctgccggaaa agccattgcc gtggtcccgc gacgtcggcg gccagccgct
    11521 cctgcgccgc cagcgcctcg atgctcagct cgaagtacgc gctgttcttc tcgtcgatcg
    11581 cgttggccca gccgaaactg tgcgcggaca gggtgtccag tggttcgtca cgcgacatca
    11641 cggtcacctg tgcgcccgcc tcggcgagcc gcagtgcggc gcacgcgccg atcgcgcccg
    11701 caccgatcac caggaccttc atgctctctc ctccgctgaa cgtgtggcgc aacgatggtg
    11761 ggtccggtgc ccgccgcgca acgtgtcctt tatggacgga tttttgatgg cttccgggac
    11821 gcgtggttga ctcgcggtcg tcgtcagcaa gcagaagccg ggcaaaaggt gtggaggatc
    11881 gcttgagcag ccgcagcgtc ctgttcgtca acctgaggcg catcaagcgc gagggcttcg
    11941 agtcactggt ggccgcccgt cgtctcggct accgcgtggt gctgctgggc cgggcgctgc
    12001 cggagttcgc tcagccgctg gtggacgagt tccaccaggt cgacacctac gacacggacc
    12061 tcgctcttaa gacggcgcgg gagatcgccg ctgccaacga cctcgccggt gtcgtgaact
    12121 tcaccgagat cgacgtccag ctcgtcgcgg cgatcgccgc cgagctcggg ctgcccggca
    12181 tgccgccgga ggccgcggtg ctggcgcgca acaagctcgc gatgaagcag gcgctcgccg
    12241 gaatcgacgg cgtgctgccg aagttcgcgc gcgtcagtga tctcgacgac ctgtgcgccg
    12301 ccgtcgcgga catcggtttc ccctgtgttg tcaaacccac cggtgcctcc gggtccaagg
    12361 ggatcttcga gctgcacggt gaaagcgatc tcgaaccggc gatggccgag ttgcagcgca
    12421 tcgcggatcc ctcgttcgac gcggtgttcc gccagttcgg tgcggagttc atcgtcgagg
    12481 agtacctgac cggagacgaa ctgtcggtcg agggcttcgt cgcggacggc accgtgcacc
    12541 agctgctgct gaccgacaag gtcaccaccg tcccgttcca cctcgaggtt tcgcaccggc
    12601 tgccgagcgt gctgcccgcc gccgcccagg accagatcct ggcgtgcagc gagaagatcg
    12661 tgcgcgcgct cgggttcgac aactgcgcct tccacctgga ggccaagtgg gacggcgagc
    12721 gcatgcggtt catcgaggtg gccgcgcggc ccgccggtga ctacatcgcc tcgcacctgg
    12781 tgaaggccgc gaccggcatc gacttcttcg ccaacgtcat ccgggtcgcg acgggagagc
    12841 cgttgcagct cgtgcccgac cgggacctgc aggcggggct gcggttcgtg ttcgccgaga
    12901 gcgccggcac gttcgacggc ctcgacggtg tcacggaact gttcgaggaa ccgggctacg
    12961 accacgtgtt caccgaagtg ccgatcggcg cccaggtcgc gttgccgccc gcgaacttcg
    13021 gctcgcagcg cgttgccgcg gtctgtgccc gcgcgcatga ccgcgccggg ctcgacgcgc
    13081 tgctcgacac cgccggagag gcgatcaaag tccggatcgg ctagtcacga ccacagccgg
    13141 gggagagggg gaaccgtgcg gctgacgttg ctgggatgtg gccggatggg gcgcggtgcg
    13201 ggctacgcgc tcgcccgcga ccagcgcacc acgtcgatca cggtcgtcga ccacgatcgg
    13261 gagcgcgcgg aggagctcgc gcgctggctc tccggatacg cggcgtgccc tgtgaagacc
    13321 ggggactcgg acgcgatcgc gggcagtgac gcggtcgcgg cggcgctgcc gtggtcggga
    13381 acgcgctcgg tgatcgaact cgccgcggcg gcgggcgttc cggtcgcgag catcacccgc
    13441 ccacccggcg acgaatcgtc cgatgtggac gcaatggcga acgccgccgg tgtcccggtg
    13501 ctgctgccgg tcggcctcga gccgggactg accgagctgg ccgccgcgga cgtcgcgcgc
    13561 aggctgaccg ctctcaccgg aggtgtgcgc acgctggagg tgttctgcgg aggcgtcccg
    13621 gccactccac gcgcgccctg ggggtacacc gcgttcttcg gcggtgagct cgcgaaccac
    13681 ctgccgatcg cgcaacgctc ctcgatcgcc gtcgagcacg gcgagatcgt cgaccacccg
    13741 cgcttctccg gggtcgagga acggcacgtg gccggcgtcg gcctgctgga ggcctaccac
    13801 gacggcatgg tgccctggct ggccgaccac ccggcgctgc gcgacgccga ctgcacgcag
    13861 aaaaccctgc gctggcccgg attcgccgac gccgtcacgg gtctggcgaa cctcgggctg
    13921 ctggacgagt caccggtcgc ggtcgacggt gtcgccgtgg ccccgaaacg ccttgtcgaa
    13981 aacgtgctgt cgccgcggtt gcgcgcgcag cccgacgacg aggacgtcgt ggtgctggac
    14041 gtgtccgcga ccggcctgcc ggacgcggac ggcagcaccc tcagcatcag gtcgctgctg
    14101 gtggatcgcg ccgaccggga gaccgggctg gccgcgatga cccgcacgac cggattcacc
    14161 ctggccgcgg cggtcacgct gctggccgac cgcacggtcg ggggcgcggg ctggctgcgc
    14221 ccgcacctgg cgttgtcgga gcggcacttc gcccgcatga agaccgatct ggccgctctc
    14281 ggcgtgcggt ggaccgccga cggagaggcc ctgcacggtc accgggcggg gacggtcgca
    14341 tcgaaaggca ctgactgatg agcaccggac acgacgtcct ggacgtggtg gggatcggta
    14401 tcggaccggc gaacctgagt ctcgcggcac tgctggccgc cgagagcagc gagctgaacg
    14461 tggcgttcct cgaccgcaag ccgcacttcg gctggcattc cggcctgatg ctgcccgacg
    14521 cgcagatgca ggtgcactac ctcaaggacc tggtgacgcc ggtcgacccg acgaacccgt
    14581 tctcgttcac ggcgtttctc gtggcgacca agcgcttcta ccggctgctc gtcaccggcc
    14641 gcagcaaggt gccgcgccgc gagttcgagc agtactgccg gtgggcggcc gagcggatcc
    14701 cgaacctgcg cttcggcgtc gaggtgcgcg aggtgacgtg gaacggcgag ctgttcgtcg
    14761 tgcacaccga ccagggcgtc ctgcacgcgc gcaacgtggt cagcggtacc ggtctggtgc
    14821 cgcggctgcc gtcgttcgcc accgggcacg atccacagga ggtgttccac gcttcgacgc
    14881 tgctcgacgt gccgagaacg ttcgcgggcc gccgggtcgc ggtggtcggc ggcgggcaga
    14941 gcggtgccga ggtcgtgcac cacctgctga cctcaccgga ccggccggcg tcgatcgtgt
    15001 ggggcacctc gcgttcgaac ctgctgccgc tggacgacag cccgttcgtc gacgagctgt
    15061 tcgtgccgaa ctacagccgc tacttccacg gtctgccggc caagcgccgg atggaactgg
    15121 tggacgagca gaagatggcc agcgacggcg tctcggtgag cctgctggag gcgatatacc
    15181 accggctcta cgacctggag atgctggagg gcgaggaacg tccgtgccgg atgctgctcg
    15241 gccattcgct gaccactgtg gacaagacgc cgaccggcct gctcacgcgg tggatgccgg
    15301 gcgccggcgc tgaagtcggt cacgaggtcg acgtcgtcat ctgcgccacc ggttaccggc
    15361 acgggttgcc gaggccgctg cacggcctgc cgcggcacct gcgcgaggcg atgtgcggcg
    15421 acgacggcga gatcaccgtg cggccggact tcagccttga ctgggaaggc ccgcaggacc
    15481 ggcggatgta cgtgcagaac gccgccaggc acagcttcgg cgtcgccgac ccgaacctga
    15541 gcctgctcgc ctggcgcagc gccaccatcg cgaacagcgt gctcgggtac gagcgttacg
    15601 acgtcggcga ggtgggagcg gtgctcgact ggacctcctc agcagattcc gacgtcgcgc
    15661 tgacgttctg accgaacagg aaccgtctga tgatcgtcag tgagatcccc cgcgagctcg
    15721 ccgacctggc gcgggcggac ttcgtgcgca gtgccgcccc ggcgtgggcc gaggcggcgg
    15781 gagtgccgtt caacgtgcgc cgggtgacgt tgcggccggg cgagacgact gccgagcaca
    15841 accaccacga cctggaggtc tgggtgatgc tcgacggtgt cggcgaggtc ggctgggacg
    15901 gccatcagcg cgtgctgacc gcgggcgaca gcgtctacct gccacctctc gcgccgcaca
    15961 ccctgcgcaa cctctccagc gaccggccct tgtcgttctt ctcgatgtgg tgggagaacc
    16021 tgtccgcgct cgctgcggtg cacgcggaac gccgggaaca ggctgtggaa cggcagggcc
    16081 gccccgtgct gctgctgccg tcgttcccca cgccgaacgg tgaactgcac ctcgggcacc
    16141 tgtccggacc gttcctcaac gctgacgcgt gccggcgtgc gttgctggcg gcgggcgaac
    16201 gcgcgcacct gctgctcggc acggtcggcc atcagagcca ggtgtccgcg gccgcggagg
    16261 cggaaggcct gtcgttccac gagctcgccg aacgcaacac cgacgcgatc atcgaaggtc
    16321 tccaggcggc gggcatcgac tgggacgtgt tcgtgcggcc gtcggagccc gcctacccgg
    16381 cgatggcgac ctcggtcttc gagtcgttgc gcgacagggg agtcctcgtc cgccgcactg
    16441 aaccgacgaa ctactgcgaa ccgtgcgggc ggttcctgct cgaagcgttc gtcgccggcc
    16501 actgcccgca ctgcgggtcg aaccagacgg ccggcatcga atgcgagttg tgcgcgttgc
    16561 cctacgacga ccgcgatctc gtcgatccgt cgtgcgccac gtgtggtgcc gccgcgacgc
    16621 aacggccact gacccggtac ttcatgccgt tggaaccact gcgggacgag ctcagcggct
    16681 acctgcgcgg tgcggcgatg cacggcaggc tgcgcgccta caccgagcgc gtgctcgcga
    16741 agacgttgcc cgacctgccg gtgagcatcc cggccgagca cggcatcccg atccacgtcg
    16801 aggacgcgtc ggggcccgcc gagcagcgga tgtactcggc gttcgagctg gccgcacggt
    16861 tcctcaccgc gctcgacgga ttcgcggacg gctgggaggc gtacgcgcgg caggagaacc
    16921 cccgcaccgt gctgttcttc ggcttcgaca acgcgttcct gcgagcgttc gcgtttccgg
    16981 cggtgctggg cgcgttcacc gatgcgctgc cgctgccaga agcgctggtg tgcaacgact
    17041 tctacctcct cgacggggag aagttctcca ccggccgcaa gcacgcggtg tgggcgcggc
    17101 aggccgtcac cccggccaac gccgaccagt tgcggctcta cctcgcggcc acctcgccgg
    17161 acgtgcgccg gcgcgacttc accacgcggg gctacgcgga gttcgtgacg gccgagttga
    17221 tcggcaggtg gcagcgccgg ctcgacgacg tcggcgggcg ggtggccgaa cacttcggcg
    17281 gcctcacccc ggaagcggga ggctggcacg ccgaggcgga acgcttctac ggtcagatca
    17341 aggagttcgc gtcgtgcgcg acgctggact acctgcccgg ccgcttcaag ccgcgtgcgg
    17401 tggtggcggc ggcctgcgcg ttcatccggc aggccgagga cttcgccgag gtgagcgcgg
    17461 acgccacccc cggctccggg atcgccagga cgtgcgcggc gctggagctg atggcgttgc
    17521 gcacgctggc catggccgtc tggccgctgg ctcccgagtt cggccggcgg gtcgccgccg
    17581 cgctcggaga ggacaccatc gcgctggagc cgacgccgcg ctgggtgcga ccggatacgg
    17641 agatcaagtt cgccaccgat cacttcagcc ccgatgaggt cgtcgcggga cgctgagcgt
    17701 gatccggacc gccgaacagc acctgggagg caggggagca tgcgcgacac cgacaccgac
    17761 accgacaccg acaccgatgc cgacgtcgcc gggccggcga gtgtggtgga cgagcgggag
    17821 ccggacatca ccggccgcag caagaaactg tggctggtgc ccggcgaccg gtgcgtcgtg
    17881 gaactggttc ccagcctgcg cagcttcacc cgcgcccggg acgagctcgt cgacgagacc
    17941 ggtccgttgc ggctggactt ctacgagaaa gccgcggcac gactgtccga tgcgggtgtc
    18001 gagtgcgcgt tccgcgcccg tctcgggccg gtcacctacc tcgccgacta ccggccggcg
    18061 ccaccgttcg aggtgatcgt caagaacgtg gcgacgggtt cgacgacacg caaatacccc
    18121 ggcctcttcc ccgacggtga gccgttgccc cgcccggtgg tcaagttcga ctaccgcgtc
    18181 gaccccgagg accagccgat cggtgaggac tacctgcggg tgctcgacct gccggtcgac
    18241 ctgatgcgcg agcaggcgct gctggtcaac gacgtgttgc gggactggct gaacccgctg
    18301 cggctgctgg acttctgcgt gatcttcggt ttctcctccg ccggtgagat cagcctgatc
    18361 tcggaggtgt cgcaggactg catgcggttg cggcacccgg acggctcgcc gctggacaag
    18421 gacctgttcc gcggcggtgc gtccgccggc gagctcgtcg agcagtggaa gagggcgttc
    18481 gatgggctct gagtcccctg tcgcactggt caccggttcg aaccggggca gcggacgtgc
    18541 catcgccgcg cggttgcacg agcgcggcta tcgggtgttc tcgttgaacc gcaccgtgac
    18601 cggcgaggac tggctgggtg agcgggagtg cgacctcgcg agtcgtgagt cgctggcggc
    18661 cggggtggcg gcggtgctgg agcgggccgg ccggctggac gtggtgatcg ccaacgcggt
    18721 cgagcgcgtg ctggacccga tcgagaagat gtccgaacag gactgggacc ggcagctgga
    18781 gatcaacctg tcgtcggtgt tccggttggt gaagcaggtg ctgccggcgt tgcgccgctc
    18841 ctgcggactg ttcctcgtga tgggcagcca cgcggggtcg cgttacttcg agggcgggag
    18901 cgcgtacagc gccaccaagg ccgcgctgaa ggcgttggtg gagacgttgt tgctggagga
    18961 gcggaacaac ggcgtgcggg cgtgtctgct ggctcccggc gcgatctcga acctggacgg
    19021 tgacgacgcg cccaccaagg tgagcgtcga gtccgtcggg acgtgcgtgg cgagcatcgt
    19081 cgcggactgg ccggccgatc tcgtggtggg ggagctggag ttccgcccgg cgctgctgcc
    19141 cgagtccccg gtgaccggca tcgaccgtct gctgcacgtc tgatgttctt cgtcgtcacc
    19201 gacctcgacg gcacgttgct caccagcgac cggctggtca ccgagcgggc gaagagcgcg
    19261 ctgacgaagg tgcgctcctc cggtggtgtc gtcgtgctga tcaccgcgag gccgttgcgg
    19321 gatgtgaccg agatcggcca ggaggtcggt gcgaacttcc tggtgtgctc gggcggcgcg
    19381 gtgctgtacg acccggtgcg cggcgagctg gtgcgcgcga ccacgctggg ctcccgccgg
    19441 gcgacgagcc tgatctcgtt gctgcgccag acgtttcccg gaatccggct cggtgtcgac
    19501 cacctggccc gctgcgacct cgaccccggc ttccacgtgg gcgcgccggg cgtcgcggac
    19561 cggcacgcga cggagccgtt gcggcaggtc gccgaaccag cggtgaagct gatcgcgcag
    19621 tccgacgaga tgccggtcga ccggctcgcg cgggcgatca gcgcgctggt cggggcggcg
    19681 tgctcggtgg cggtgccgtg cagctacttc gttgacgtgc tgcccgccgg ggtgcacaag
    19741 gccgtcggac tcggcgagct gcacgagtgc cacggccgcg tgctgccgtc gatcgccttt
    19801 ggtgacatgc cgtcggatct gccgatgttg aggtgggcgg acgtctcggt cgccacggcg
    19861 aacgcgcacc ccgcggtgct ggaggcgtgc gaccacgtca cggcgcacca cgacgccgac
    19921 ggtgtcgctg tctatctgga aagcctgatc agcactgggg gatgaggaaa tggtcactct
    19981 cgtgctgccc acggggtcgt tgcacgagcc gactctgcgc ctgttcgacg cggcgggcct
    20041 gaccgtccac cggcaggcgt cgcgggcgct gcgggcacac gtcgacttcc ccggcatcga
    20101 gcgggtggtg ttcggcaaac ctcgcgagat tccgggtctg gtcgcggacg gcgtcgtcga
    20161 cctcgggctg accggcacgg actggatcga ggagagcggc gccaaggtcg aggtggtgca
    20221 cgagttccag tactcgaaga ccaccagcgc cggttggcgc gtcgtgctcg ccgtaccggt
    20281 cgaccatccc gcgcggaccg cggcggatct gcccgcgggc gtacgcgtcg cgtcggagta
    20341 ccccgccatc gcacggcgct acttcgagga ggaactgggc caggaggcgc gcatcgtgca
    20401 ctcgcacggt tcgaccgagg cgaaggtgcc ggatctcgcg gacgctgtgc tggagatcgt
    20461 ggagaccggg gacacgttgc ggcacaacga cctacgcgag ctggtggtcg tgcggcgatg
    20521 cgcggtgcag ctggtggtcg ccacgcaggc ggaccccgtc gtgcgcgcta cggcggagaa
    20581 ggtcgcgttg ctgctcaagg gcgcgcgggc agccacggag cacgcgctgc tgaccgtggt
    20641 ggcgcccgct gactgctggg accgggtgcg gcaggaactg ccgcggtact ggtggctgct
    20701 cggcggagac ccggaaaccg tggtggcaca agcgacctac gagcttcggg gcgtcgcgga
    20761 agcgatcacg cggctcacac aggccggtgc ggtgcaggtc gtggagacgc cgatgcgaaa
    20821 gctggtgacg gcgtgacagg tctccggagt tcctgccgtt caacggtcct cgatgtcatg
    20881 tttgcgcaga atccactgtg gtgagagagg ttttccaatg gccaccaaat ccatgaacga
    20941 gactctgacc cgagtcggca ggggcacgcc catgggcgag ctgctgcgcg agtactggat
    21001 gccggtgatg cggtccgagc gcgtcgtcgc gggtggtgaa cccatcgctg tcgagctgct
    21061 cggcgaccgg tacgtgatct tccgcggcga cgacggcacc gtcgcctgct tcgacgaggc
    21121 gtgcccgcac cgcggcgctt cgctcgcgtt ggcgcgcaac gaggactgtg cgctgcgctg
    21181 catctaccac ggctggaagt tcacgaccga cggccggacc gtcgagacgc cctccgaacc
    21241 ggaggacgga gggcggttcg cgtccaaggt gaagctcaac caccaccccg tcgtcgacgc
    21301 gggcggcgtg atctgggtgt gggccggcgg caccggtcgt gagccgagcc cgttcccgcg
    21361 cttcgccttc accgacctgc cctcctcgca cgtgttcggc atcgtggcga tcctcgactg
    21421 caactgggtg cagggactgg aagcggacat cgactccgcg cacgtgtcgc tgttgcacga
    21481 gaccgaggcg gcgaagggtc cgctgaagga tctgctcgac gaccgcgcgc cacgcgacga
    21541 gatcgagaag acctcctggg gcatccgcta cgcggcgatc cgccgactgt ccactgggga
    21601 cagcctggtg cgggtgaaac cgatggtcat gccgtggtac accatcgtgc ccgagcttcc
    21661 caacggtgac cgcctgtggc acgcgtgggt gccgatcaac gaccaccaga ccatcttctg
    21721 gtacctctgg tacaacgagg accagccggt cgacccgaac tacttcgccg accagttcgg
    21781 cctggacctc gagaacatga acaaggacaa cttccgcgag ggttactcgc ggaagaacat
    21841 gtggggccag gaccgcgccg cgatgcgcgc gggcacgtcg ttctccggca tcagcggcct
    21901 cgccgcccag gacatcgccg tgcaggagag catgggcgcg atcgtggacc gttcgatcga
    21961 gaaccccggc aagagcgaca ccggtctcgt ccgggcacgc cacttcctgc tggacgcgat
    22021 caaggacaag gcggagggaa gggtgccggc cgggctcggc gacgaggtcg actaccgcaa
    22081 gatctcgtcg gcgaacgtcg tggtgagcga cggggacgac tggcgtcagc tggtggtgta
    22141 gcacggtgcc ggtgcgggtg acggtgtcag cgcagttcgg ccgcgactgc cgggcgtggc
    22201 gtcatcctgc ccggcatcgc gcgcaccgtc ccggcggtca ccacgacggt gatcgccggg
    22261 atgtcgccga cgcgcatggc ctccgccacc gtgtccgcct gggaaccagc gggcgccgcg
    22321 agacccagca gatccgcggg cgcgccccgg cgcacccgtc cttccggtag gtgccacacg
    22381 tcggcaaccg tgcccgtcgc gagcgaccac gccacctcgg gttcgagccc gccgagcgcg
    22441 gtcagctcca gcaccgtctt cagcaccgcg agcggcatca caccgaaccc ggacggcgtg
    22501 tccgagccga ccaccacgcg gtgcagctcg tcgcgggacg ccgcatgctc cacgatgcgc
    22561 agcgccgcct tgaggttgcc cgcctgcacc acctgcaacg ccatgtccgt ctcggcgaac
    22621 aacgcctcga cctccgcgaa cggcaacgca gtcggcccgc cgttcgcgtg cccgcacacg
    22681 tcgggccgca acgcgagcaa cgcctcgcct cccagcgagg ccccacccgc ggcactcgcc
    22741 ccgcccgcgt ggcacatcac ggtcagcccg gcctcccgcg cccagcgcac gtgctcgacg
    22801 ccgtcgaccg gatcggtgaa cgcgccgaac ccgtacttcg cgagccgcac cccggtcgcc
    22861 gcgagctccc ggaagtcctc ttcggacagc gtaggctcga tcagcaccgc gcccgcgcgc
    22921 acccgcatgc cgccgggccg gtactcgctg aagcaccgct gcgcggcgac ggcaagcgct
    22981 ttcaccccgg ccctgtcgcg tggccgtccc ggcacgtgca cctcgcccgc cgacaccgtc
    23041 gtcgtgatgc cgccctggac gtacccggcg aggtaatcga ccgcgcgctg ccgcgggctg
    23101 tagtccccga acgtcacgtg cccgtgtgag tcgatcagtc ctggcgcgat cgtgccgccg
    23161 cgcaggtcga tcacctcgtc ggcgccgtcg atctcgtgcg tcaggctgct cgccttcccc
    23221 gtcgcgtgga tcatgccgtc ccggcagacc acggtgtcgg cgtcgagcag cggtaccgcg
    23281 agatctccgg agaaaacggt tgccgcgccg gtcagcacga gcgttgccat gcgtcctcca
    23341 catcggactc gagtgcgaca aagccgcgt
//
