LOCUS       BGC0001986              3657 bp    DNA     linear   BCT 01-SEP-2019
DEFINITION  Streptomyces chrestomyceticus strain NA4264 pcm gene cluster,
            complete sequence.
ACCESSION   BGC0001986
VERSION     BGC0001986
KEYWORDS    .
SOURCE      Streptomyces chrestomyceticus
  ORGANISM  Streptomyces chrestomyceticus
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-08 05:49:53
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            1..345
                     /gene="pcmC"
     CDS             1..345
                     /codon_start=1
                     /gene="pcmC"
                     /note="PcmC"
                     /product="cyclodipeptide oxidase"
                     /protein_id="QEI22733.1"
                     /transl_table=11
                     /translation="MAPGHPRPTTAPDLTDDIVLLAAFLLSSAHGLLDEPPAYGPARCA
                     DGARRALELLDAYGEPDPALLNVRTRLEDAMCGSMADVDLPTLLRTACEQMLDVVTARR
                     AGAPHGPAEG"
     source          1..3657
                     /db_xref="taxon:68185"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces chrestomyceticus"
                     /strain="NA4264"
     misc_feature    1..3657
                     /note="pcm gene cluster"
     protocluster    1..3657
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[2181:2952]"
                     /cutoff="5000"
                     /detection_rule="(CDPS or cycdipepsynth)"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2182..2952
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(CDPS or cycdipepsynth)"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     cand_cluster    1..3657
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(CDPS or cycdipepsynth)"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..3657
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001986"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..3657
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="CDPS"
                     /region_number="1"
                     /rules="(CDPS or cycdipepsynth)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            346..945
                     /gene="pcmB"
     CDS             346..945
                     /codon_start=1
                     /gene="pcmB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00881"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1159:dihydropteridine reductase (Score: 37.5; E-value:
                     2.8e-11)"
                     /gene_kind="biosynthetic-additional"
                     /note="PcmB"
                     /product="cyclodipeptide oxidase"
                     /protein_id="QEI22734.1"
                     /sec_met_domain="PF00881 (E-value: 7.9e-16, bitscore: 47.0,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTCPAGTTPEAAGSAAARTAFLELLRTRAVVRRYTREPVDDTLLD
                     ALLDVMPAAPTAANKQAWGFVAVQDPYTVRCLRAFAPGMIGLPPLVVTACFDRDRAARE
                     DSGPTDIGLLCVAMAVQNLLLAAHATGLGGCPVSSFSRTAVRRLLALPPRLEPVLLVPV
                     GHPADPVRPSARRHRDEVIRHGTWTPPADDSTGPDR"
     gene            946..2181
                     /gene="pcmD"
     CDS             946..2181
                     /codon_start=1
                     /gene="pcmD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007:cytochrome P450 (Score: 301.3; E-value: 1.7e-91)"
                     /gene_kind="biosynthetic-additional"
                     /note="PcmD"
                     /product="P450"
                     /protein_id="QEI22735.1"
                     /sec_met_domain="p450 (E-value: 2.3e-15, bitscore: 44.6,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGQPPGERTAHPAGTDVAFPLSRRGDLVPDECAWLRDERPVARV
                     RTLAGDPAWLVSTYELATRVLDDETFSLSATAAPGARQQYAPTFPIEIRKNLSRIKDEG
                     MRDAVMQALSPRAIKEATGQFRRRAEALIDEVLAEGPPVDLKDRFTDPYTAHVMCTVLG
                     LPDDDWRRLMSGLDISIMTAPRPFEGAMANWDKGMAYMAGLLRAPGATEAPGLLGALAR
                     VRADSGGEEGADTDEHLTMTLHSLFEAGAVSTSAFLTLAIMLLLRDTGQRRWLGDHPEA
                     IGPAVEELLRYNLSIGDALPRIATRDTELGGTPVAAGDLVLVLVEGANHDPAVFRDPGR
                     LDLTRTPNPHLAFGSGRHYCPATTLARAHAATALTALLARLPGLRLAVPDEEIGWRSGW
                     IKRTPERLPVLW"
     gene            2182..2952
                     /gene="pcmA"
     CDS             2182..2952
                     /codon_start=1
                     /gene="pcmA"
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     cycdipepsynth"
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /note="PcmA"
                     /product="cyclodipeptide synthase"
                     /protein_id="QEI22736.1"
                     /sec_met_domain="cycdipepsynth (E-value: 8.2e-73, bitscore:
                     233.0, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="CDPS (E-value: 2.3e-70, bitscore: 225.1,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGAPQAAVNFRTRPLTDRCDRIRRRGDHALIGVSTNNSYFSAQRL
                     TALVHWAQGQFRAVDIICADLHIDTVLTAEGAAPESVGKRARRRVTDVRRRIRKAVQAA
                     SPDGPQPGTHLLSDFQHNDAYQRLRADIDRALREDPEFADACHDMVRLHMLGQPQKAGG
                     AHSAAGSGDGGPADGAEAGRLRAGLQYLCGELPFFVDTPSILGVPSSVSCYHLFTPVLG
                     PLCMRENGLRAADNQAFLAVTPDDAPTDGERDVT"
     gene            2953..3657
                     /gene="pcmE"
     CDS             2953..3657
                     /codon_start=1
                     /gene="pcmE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1248:methyltransferase (Score: 79.8; E-value:
                     3.6e-24)"
                     /gene_kind="biosynthetic-additional"
                     /note="PcmE"
                     /product="methyltransferase"
                     /protein_id="QEI22737.1"
                     /transl_table=11
                     /translation="MKRIGKGELRMTAEEMTPYGREAARVADQVAARYAEVAPAHQHSQ
                     IAVVERLLTRLPRGARALDAGCGTGRPTCAQLCAGGLDVTGIDVSEAMLAAARANVPQA
                     RFLQLDLLGEQATGLGTFDAVVSFFCLMNLPEPVFVSALARFASLAGRDGTVVVGVPER
                     PGDTPVEGLYGTYQPPRCTRQDLRHRAERAGLAVEEIEVRPGTGLMGAEEEHLFLYARP
                     VPPGASPSSHSG"
ORIGIN
        1 atggcacctg gacacccccg gccgacgaca gcaccggacc tgaccgatga catcgtcctg
       61 ctggccgcct tccttctcag cagcgcccac ggcctgctgg acgaaccccc ggcgtacgga
      121 ccggcccgct gcgccgacgg cgcccggcgc gccctcgaac tgctggacgc gtacggggag
      181 ccggacccgg cactgctgaa cgtccgcacg cggctggagg acgcgatgtg cggctccatg
      241 gccgacgtgg acctgccgac cctgctccgt acggcgtgcg agcagatgct ggacgtcgtc
      301 acggcccgcc gggcgggcgc gccgcacggg cctgccgagg gctgagtgac ctgtcccgcc
      361 gggaccacgc ccgaggcggc cggctccgcg gcggcacgga cggcgttcct ggaactgctg
      421 cggacgcggg cggtggtgcg ccgctacacc cgcgaaccgg tcgacgacac gctgctcgac
      481 gcactgctgg acgtcatgcc ggccgcgccc acggccgcca acaaacaggc atggggtttc
      541 gtggccgtcc aggaccccta caccgtgcgc tgtctgcggg ccttcgcgcc gggcatgatc
      601 ggcctgccgc cgctggtggt gacggcgtgc ttcgaccgcg accgcgccgc ccgcgaggac
      661 agcgggccca cggacatcgg cctgctctgc gtggccatgg ccgtacagaa cctgctcctg
      721 gccgcgcacg cgaccggtct gggcggctgt cccgtcagca gtttctcccg gaccgccgta
      781 cgccgcctgc tggcgctgcc gccccgcctc gaacccgtcc tcctcgtacc ggtcggccac
      841 ccggcggacc cggtgcgccc ctccgcccga cgccaccgtg acgaagtgat ccgccatggc
      901 acctggacac ccccggccga cgacagcacc ggacctgacc gatgagtgag cggacagcct
      961 cccggcgaac ggaccgccca cccggccggc acggacgtgg ccttcccgct ctcccggcgc
     1021 ggtgacctcg tccccgacga gtgcgcctgg ctgcgcgacg agcgcccggt cgcccgggtg
     1081 cgcacactgg ccggcgaccc ggcgtggctg gtgtccacct acgagctggc cacccgcgtc
     1141 ctggacgacg agaccttcag cctctcggcc accgctgccc ccggcgcgcg ccagcagtac
     1201 gcgcccacct tccccatcga gatccgcaag aacctgtcgc gcatcaagga cgaggggatg
     1261 cgggacgccg tgatgcaggc cctgtcgccc cgggcgatca aggaggcgac cggccagttc
     1321 cgccgccggg ccgaggcgct gatcgacgag gtgctggccg aggggccgcc cgtcgatctc
     1381 aaggaccggt tcaccgaccc ctacaccgcg cacgtgatgt gcaccgtcct cgggctgccc
     1441 gacgacgact ggcggcggct gatgagcggc ctggacatca gcatcatgac cgcgccccgt
     1501 cccttcgaag gcgctatggc caactgggac aagggcatgg cctacatggc cgggctgctg
     1561 cgggcgcccg gcgcgaccga agcgcccggc ctcctcggcg cgctggcacg ggtccgcgcc
     1621 gacagcggcg gcgaggaggg cgcggacacc gacgagcacc tcacgatgac gctgcactcc
     1681 ctgttcgaag cgggagccgt cagcacctcc gccttcctga cgctggcgat catgctgctg
     1741 ctccgcgaca ccggacaacg gcggtggctg ggggaccacc ccgaggccat cggcccggcg
     1801 gtggaggaac tgctgcgcta caacctgtcc atcggtgacg cactgccgcg catcgccacc
     1861 cgggacaccg aactgggcgg tacccccgtc gccgcgggcg atctcgtcct cgtcctggtc
     1921 gaaggcgcca accacgaccc ggccgtcttc cgcgacccgg gccggctgga cctgacccgc
     1981 acgcccaacc cccacctggc gttcggcagc ggacgccact actgcccggc caccacactg
     2041 gcccgggccc acgcggccac cgcgctcacc gccctgctcg cgcgccttcc cggcctgcgc
     2101 ctcgccgtgc ccgacgagga gatcggctgg cgctccggct ggatcaagcg cacccccgaa
     2161 cgcctgccgg tgctctggtg aatgggtgcg ccgcaggctg cggtaaattt tcgcacacgt
     2221 ccactcactg accggtgtga ccgtattcgc cggcgtggcg atcacgcgtt gatcggagtg
     2281 agcacgaaca acagctattt cagtgcgcaa cggctcaccg cgttggtgca ctgggcgcag
     2341 gggcagttcc gggccgtcga catcatctgt gccgacctcc acatagacac cgtgctgacc
     2401 gccgaggggg cggctccgga gagtgtcgga aagcgcgctc gcagacgcgt caccgatgtg
     2461 cgccgcagaa tccgcaaagc cgtccaggcg gcctcgcccg acggtccgca gcccggtacc
     2521 catctgttgt cggacttcca gcacaatgac gcctaccagc gtctgcgggc ggacatcgac
     2581 agggccctgc gcgaggaccc ggaattcgcg gacgcctgcc acgacatggt ccgtctccat
     2641 atgctgggac agccgcagaa agcgggcgga gcgcattccg ccgccggctc cggggacggc
     2701 ggcccggcag acggggcgga agccggccgg ctacgggccg ggctgcagta cctgtgcggc
     2761 gaactgccat tcttcgtgga caccccgagc attctcggtg ttccctcgtc ggtttcctgc
     2821 taccacctgt tcacgcccgt cctcgggccg ctgtgcatgc gggagaacgg cctgcgggcc
     2881 gcggacaacc aggcgttcct ggcggtgacg cccgacgacg cccccacgga cggagaaagg
     2941 gacgtgacgt gaatgaagcg catcgggaaa ggcgaactcc gcatgacagc agaggaaatg
     3001 actccgtacg gcagagaggc cgcgcgagtg gccgatcaag tcgccgcgcg ctatgccgaa
     3061 gtggcacccg cccaccagca ttcccagatc gccgtcgtcg agcggctgct gacgcgcctg
     3121 ccacgcgggg cccgggcact cgacgcgggc tgcggcaccg gacggcccac ctgtgcgcag
     3181 ttgtgtgcgg gcgggctgga cgtcaccggg atcgacgtgt cggaagcgat gctggcggcg
     3241 gcgcgcgcga acgtacccca ggcccggttc cttcaactgg acctgctcgg tgaacaggcc
     3301 accgggctgg gcacgttcga cgccgtggtc agcttcttct gcctgatgaa ccttcccgag
     3361 ccggtgttcg tctcggcgct ggcccgcttc gcctcgctcg ccgggcgcga cggaaccgtc
     3421 gtcgtcggcg ttcccgaacg ccctggtgac acacccgtgg agggccttta cgggacgtat
     3481 cagccacctc ggtgcacccg ccaggacttg cggcaccgcg ccgagcgggc cgggctggcg
     3541 gtcgaggaga tcgaggtccg gccgggcacc ggcctcatgg gcgcggagga ggaacacctc
     3601 ttcctgtacg cccggccggt cccgccgggg gcctcgccct catctcactc cgggtag
//
