LOCUS       BGC0001992              4749 bp    DNA     circular CON 24-DEC-2022
DEFINITION  Erwinia amylovora ATCC 49946, complete sequence.
ACCESSION   BGC0001992
VERSION     BGC0001992
KEYWORDS    RefSeq; complete genome.
SOURCE      Erwinia amylovora ATCC 49946
  ORGANISM  Erwinia amylovora ATCC 49946
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Erwiniaceae; Erwinia.
REFERENCE   1
  AUTHORS   Sebaihia,M., Bocsanczy,A.M., Biehl,B.S., Quail,M.A., Perna,N.T.,
            Glasner,J.D., DeClerck,G.A., Cartinhour,S., Schneider,D.J.,
            Bentley,S.D., Parkhill,J. and Beer,S.V.
  TITLE     Complete genome sequence of the plant pathogen Erwinia amylovora
            strain ATCC 49946
  JOURNAL   J Bacteriol 192 (7), 2020-2021 (2010)
   PUBMED   20118253
REFERENCE   2  (bases 1 to 3805874)
  AUTHORS   Aslett,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2010) Aslett M.A., Wellcome Trust Sanger
            Institute, Pathogen Sequencing Unit, Wellcome Trust Genome Campus,
            Hinxton, Cambridge, Cambridgeshire. CB10 1SA, UNITED KINGDOM
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/24/2022 02:15:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,570
            CDSs (total)                      :: 3,448
            Genes (coding)                    :: 3,367
            CDSs (with protein)               :: 3,367
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 22
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 45 of 81
            Pseudo Genes (incomplete)         :: 45 of 81
            Pseudo Genes (internal stop)      :: 30 of 81
            Pseudo Genes (multiple problems)  :: 30 of 81
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: False
            Run date                          :: 2025-08-15 20:41:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1133806
            Orig. end                         :: 1138555
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FN666575.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..4749
                     /contig_edge="False"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     subregion       1..4749
                     /aStool="mibig"
                     /contig_edge="False"
                     /label="other"
                     /subregion_number="1"
                     /tool="antismash"
     gene            complement(1..876)
                     /locus_tag="EAM_RS05030"
                     /old_locus_tag="EAM_1028"
     CDS             complement(1..876)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /function="Transcriptional regulator"
                     /gene_functions="regulatory (smcogs) SMCOG1031: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004169025.1"
                     /locus_tag="EAM_RS05030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAM_1028"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_004156381.1"
                     /transl_table=11
                     /translation="MSNLPSLRALHYFHQAALRQSFSRAAQSLNVTHSAVSHQIKQLEE
                     WLGKPLFLRTKGRVQLTADGERLQACCVQSFRSIEATCDKIRVCDLHSLTISCAPSFLA
                     QWLIPRINRFTSRHSEIALTFQTHVDLEKVRSEHTDVLILSQEQPVQKDIAATLITVDY
                     IGPVCSPAFSSRFSYETDFSTLPLLHADTKLNAWSEWAKKTGARGDFWAGNHFDNLTLA
                     IQAARNGMGIIMTPQILVKKEIFDGSLIAPLGFAEVDRATWMMIKASRKADKGIELFRS
                     WLLEEASMME"
     gene            1082..1786
                     /locus_tag="EAM_RS05035"
                     /old_locus_tag="EAM_1029"
     CDS             1082..1786
                     /codon_start=1
                     /function="Predicted
                     tRNA(5-methylaminomethyl-2-thiouridylate)
                     methyltransferase, contains the PP-loop ATPase domain"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004156383.1"
                     /locus_tag="EAM_RS05035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAM_1029"
                     /product="hypothetical protein"
                     /protein_id="WP_004156383.1"
                     /transl_table=11
                     /translation="MTNQYSVKPQLVMFTGGRDSTLAACYLMLQGIPVHLWSGNSGCSL
                     HRGILSHRVEEMKNRFGDLVVGHTVADISGAFRSIAIEALEQDILKYRKNLVLLGEKLA
                     IHAHLVDFCRRNDINTINDGITHYQMEFPEQRLVAKTFLMEMMAQYDINYQSPVYEFAQ
                     SADDVKYRLLQLGISTKSLEGISIFADSFSTPSDDVVLAYLREKAPLAHNIVNFLAGET
                     LNPPVLNNSAAA"
     gene            1839..2237
                     /locus_tag="EAM_RS05040"
                     /old_locus_tag="EAM_1030"
     CDS             1839..2237
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012442180.1"
                     /locus_tag="EAM_RS05040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAM_1030"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_004156386.1"
                     /transl_table=11
                     /translation="MKTIVKCAAIIIHQRSLLLTRKRGTAIFISPGGKPLAGEDHLSCL
                     KRELDEELGVQIKSFRPFGLFHGRAEFEAQAIENHVYFVEIIGQPRAGNEIEEITWVNY
                     RKPPCEIAVGSIFRNNVMPLLFQQELIN"
     gene            2237..3112
                     /locus_tag="EAM_RS05045"
                     /old_locus_tag="EAM_1031"
     CDS             2237..3112
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004169026.1"
                     /locus_tag="EAM_RS05045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAM_1031"
                     /product="DUF6024 family protein"
                     /protein_id="WP_004156388.1"
                     /transl_table=11
                     /translation="MDASSLSPQLQQVHGDRFYKRVNMLKDELREELTRVYRLHDYDLF
                     FVQSVRVGLVILSHLFYKQETSLCLAKHAHYKPISELFNSSSAHASVPGSVPIITHVSP
                     YTGEVNSLQDCKGKGVVDASHSFATNRHAELIAESSVFVAPLHKHASLTVGLAIVALRS
                     EHFSTLMRSELRLFEESTSSDKPLEEALDNVRSHSWQPFNVAQVRPVEIKDAAGMDFCS
                     ISAPDLPFCCFPVPTLSATRQQQVKKAGATYFPHTNTVRLSCWVRGDGSQRVDMTKQVE
                     QDLIALWEGK"
     gene            3109..4011
                     /locus_tag="EAM_RS05050"
                     /old_locus_tag="EAM_1032"
     CDS             3109..4011
                     /codon_start=1
                     /function="Permeases of the drug/metabolite transporter
                     (DMT) superfamily"
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004169027.1"
                     /locus_tag="EAM_RS05050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAM_1032"
                     /product="DMT family transporter"
                     /protein_id="WP_004156390.1"
                     /transl_table=11
                     /translation="MKGSMAVNGNLIGMAGGVILSTDAVFIRLMGINNSWLIVTLRGIC
                     MWTICLLIWVFWRQSRSTLGQPWLNRDNLLSTLFFCIASACFVNALNRGNVATVLVIIS
                     GTPFISALMSRLLFKVAIDRSVMLAALAGIVGVSIVMSGNSMGHNGVANLYALATAVSM
                     ALAFIFTSRVSGGTAGLPSLGALLASVLIVLGSGSDVVISLKMLSAAQLGWVIAEGTLI
                     MPLAMGLITLSTRFVSPANAGLFLLLETALAPLWMYLFLGEVPTVQAVIGGAIIIITVM
                     TQSLYARQKESASRCADMA"
     gene            4105..4749
                     /gene="adk"
                     /locus_tag="EAM_RS05055"
                     /old_locus_tag="EAM_1033"
     CDS             4105..4749
                     /EC_number="2.7.4.3"
                     /GO_function="GO:0004017 - adenylate kinase activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /function="Adenylate kinase and related kinases"
                     /gene="adk"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415007.1"
                     /locus_tag="EAM_RS05055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAM_1033"
                     /product="adenylate kinase"
                     /protein_id="WP_004156392.1"
                     /transl_table=11
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKAGSEL
                     GQKAKEIMDAGKLVTDELVIALVKERIAQEDCRKGFLLDGFPRTIPQADAMKEAGIKVD
                     NVLEFDVPDALIVERIVGRRVHVSSGRVYHVTFNPPKVAGKDDVTGEELSTRKDDQEET
                     VRKRLVEYHQMTAPLIAYYSKEAAAGNTAYHKIDGTRHVTEVSAELAKILG"
ORIGIN
        1 ctactccatc atcgaagctt cttctaatag ccagctgcga aataattcaa tccccttatc
       61 cgccttgcgt gacgctttta tcatcatcca ggtagcgcga tccacctcgg caaaacccag
      121 gggtgcaatc agtgaaccgt cgaagatctc ttttttcacc aggatctgcg gcgtcatgat
      181 aatccccatg ccgttacgcg ccgcctgaat cgccagagtc agattgtcga aatgatttcc
      241 agcccagaaa tcaccgcgcg ctccggtttt tttcgcccat tcggaccagg cgttgagttt
      301 ggtatcggca tgcagcagcg gtaaggtaga gaaatcggtc tcgtagctga agcggctact
      361 gaacgctggg gaacataccg ggccaatata gtcgacggta atcagcgtgg cggcaatgtc
      421 cttctgtact ggctgctcct gactgaggat cagtacgtcg gtatgctcac tccgcacctt
      481 ctccagatcg acgtgggtct ggaaagtcag ggcgatttcg ctatgacgtg aggtgaagcg
      541 gttgatacgc ggaataagcc attgcgccag aaaactcggg gcgcacgata tcgtcaggct
      601 gtgaagatcg cacacgcgaa ttttgtcaca ggtcgcttct atactgcgaa agctttgcac
      661 acagcacgct tgaagccgct caccgtctgc cgttaattgc acccggccct tagtccgcag
      721 aaataaaggt tttcccagcc actcttctaa ctgtttgatt tgatgactga cggcactgtg
      781 cgttacgttc agcgattgtg ccgcgcggct aaaactctgc cgcagcgcag cctgatggaa
      841 atagtgtaat gcgcgcagtg agggaaggtt agacattatc gtaccttgtt agaaaaattc
      901 acattaagtg tctgtttata tcatttttaa gtccagccaa aattcactag tctggcacag
      961 gttccgggga atgtactggt agcgaacagc ctgaatcaac tgatggaatc tcacttaaca
     1021 cagacgaatt ttccccttgt cagacctcgt taaaactcaa cagctatgcg aaggaaagga
     1081 aatgacaaat cagtattctg ttaagccgca actcgttatg tttaccggtg gtcgggatag
     1141 caccctggcc gcttgttatc ttatgttaca gggcattcct gtccacctgt ggtcgggtaa
     1201 tagcggatgc tcactgcatc gcggtatttt gtcgcaccgt gttgaagaaa tgaagaatcg
     1261 ctttggcgat ctggtcgtgg gtcataccgt tgccgacatt agcggagctt ttcgctctat
     1321 tgccatcgaa gcgctggaac aggatattct caaatatcgc aaaaatttgg tgctgctcgg
     1381 cgaaaaactg gccattcatg cgcatctggt cgatttctgc cgccgcaacg atatcaatac
     1441 catcaacgat ggcattacac attatcaaat ggagttcccg gagcagcgac tggtggcaaa
     1501 aaccttcctg atggagatga tggcacaata tgacatcaac tatcagtcgc cggtctatga
     1561 gtttgcgcag tcggcagatg acgtgaaata tcgtctgttg cagctgggta tctcgacgaa
     1621 atcccttgag gggatcagca tctttgccga cagtttctct acgccttcag atgatgtcgt
     1681 gctggcttat ctgagagaaa aagcgcctct ggcacataat attgtgaatt tccttgctgg
     1741 cgaaacgctt aatcctcccg ttttgaacaa cagcgccgca gcctgaggct gtacgaaaat
     1801 cgtgacgtaa tctgcggatt acgtctttcg gagttgagat gaaaacaatt gttaaatgcg
     1861 ctgccattat tattcatcag cgctcgctgt tacttacccg taaacggggc accgcgattt
     1921 ttatttctcc cggtggcaaa ccgttggcag gggaagatca tctgagctgc ctcaagcgag
     1981 aattagatga agagctgggt gtgcaaatca aaagtttccg accttttggg ctgtttcatg
     2041 gtcgcgctga attcgaagca caggccatcg agaaccacgt ttattttgtc gaaattattg
     2101 gccagccgcg cgccggcaat gaaattgaag agattacatg ggtaaattat cgcaaaccgc
     2161 cctgtgaaat tgcggtcggg tcgatttttc gcaataacgt tatgccactg ttattccagc
     2221 aagagttgat caactgatgg atgcctcctc cctttccccg cagttgcagc aggtgcacgg
     2281 cgatcggttt tataagcgag tcaatatgct aaaagacgaa cttcgtgaag agcttacacg
     2341 ggtataccga ctacatgatt acgatctgtt tttcgtccag tcggtgcgcg ttgggttagt
     2401 gatcctttca cacctgttct acaagcagga aacctcttta tgcctggcga agcatgcgca
     2461 ttacaaaccc atcagtgaac tgtttaactc atcttcagcg catgccagcg tgccgggcag
     2521 cgtaccgatt attacccacg ttagccctta cacgggggaa gttaacagtc tgcaagattg
     2581 caaagggaaa ggggttgttg atgcgtctca cagtttcgcc actaatcgcc acgccgagct
     2641 gatcgcagag agttcggtgt ttgtcgcccc cttgcataaa cacgcttcat tgaccgttgg
     2701 gctggcgatc gttgctttac gctctgaaca cttcagcacg ctgatgcgta gcgaattacg
     2761 cctgtttgaa gagtccacct cttcagacaa gccgctggaa gaagcgctcg acaatgtgcg
     2821 cagtcacagc tggcagccct ttaatgttgc ccaggttcgg ccggttgaaa tcaaggatgc
     2881 agcggggatg gacttttgct ctatcagcgc accggacttg ccattctgct gcttccctgt
     2941 acccacgcta agcgccacac ggcaacagca ggttaagaaa gccggtgcca cttatttccc
     3001 ccataccaac acggtgcgat tgtcgtgttg ggtgcgtggc gatggcagtc agcgggtgga
     3061 tatgaccaaa caggtagagc aagatttgat tgcattatgg gagggaaaat gaaaggttcc
     3121 atggcagtaa atggcaatct gattggcatg gcgggcggcg ttatcctgag caccgatgcg
     3181 gtattcattc gtctgatggg aatcaataac tcatggctga ttgtcaccct gcgcgggatc
     3241 tgcatgtgga ccatttgcct gctgatctgg gtcttctggc gtcaaagccg ttcaacgctg
     3301 gggcagccgt ggctgaacag agataatttg ctttcgacgc tgtttttctg catcgcttcc
     3361 gcctgtttcg ttaatgcact gaaccgtggc aacgtggcaa ctgtactggt tatcatctcc
     3421 ggtacgccgt ttatttcggc gctgatgagc cgtctgttgt ttaaagtggc gatcgatcgc
     3481 agcgtgatgc tggctgcact ggcaggcatt gttggcgtga gtatcgttat gtccgggaac
     3541 agcatgggtc acaacggcgt ggccaatctt tatgcgctgg ctacggcagt atcgatggcg
     3601 ctggcgttta tttttacttc gcgcgtaagc ggcggaacgg ccgggctgcc atcgctggga
     3661 gcgctgctgg cttcggtatt gatcgtcctt gggtcaggtt ccgatgtggt catcagtctg
     3721 aaaatgctgt cggcagcaca gctgggatgg gtcattgctg aaggcaccct gatcatgccg
     3781 ctggcgatgg ggttaattac cttatcaaca cgttttgtct cgcctgctaa tgccggactg
     3841 ttcttactgc tggaaaccgc gttagcgcca ctatggatgt atttgttctt gggtgaagtg
     3901 cccacagttc aggcagttat tggcggtgcg atcattatca tcacggtgat gacacagtcg
     3961 ctatacgccc gtcaaaagga gtctgcatcg cgctgcgccg atatggcata accgtgccct
     4021 tccttgtgaa aaacggggca tgatgatatg ttcgtgccct cagataatgt gttcatttaa
     4081 gcaattcgca aggggattta cgcaatgcgt attattctgc tcggggctcc gggtgcagga
     4141 aaggggactc aggctcagtt cattatggag aaatacggta ttccgcaaat ctctacgggt
     4201 gatatgctgc gcgccgcggt taaagccggt tcggagctgg gtcagaaagc taaagagatt
     4261 atggacgccg gtaagttggt taccgacgaa ttggttatcg cactggtcaa agagcgtatc
     4321 gctcaggaag actgccgtaa gggtttcctg ctggacggtt tcccgcgcac cattccccag
     4381 gctgatgcga tgaaggaagc gggtatcaag gtcgacaacg tgctggaatt tgatgtgccg
     4441 gacgcactga tcgttgagcg tatcgtcggc cgtcgggtgc atgtgtcgtc gggtcgggta
     4501 taccatgtta ccttcaaccc gccgaaagtg gcaggcaaag atgatgtgac cggcgaagag
     4561 ctcagcacgc gcaaggacga ccaggaagaa accgtgcgta aacgcctggt ggaatatcat
     4621 cagatgacgg ctccgctgat tgcttattac agcaaagaag ctgcggcagg taatacggcc
     4681 taccacaaaa tcgatggtac gcgtcatgtg acagaagtca gcgccgaact ggcaaaaatt
     4741 ctcgggtaa
//
