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Overview
1.1

### Identified secondary metabolite regions using strictness 'relaxed'

BGC0002009 (Amycolatopsis vancoresmycina)

Region | Type | From | To | Similarity Confidence | Most similar known cluster
--- | --- | --- | --- | --- | ---
Region 1 | T1PKS,NRPS-like | 1 | 113,783 | High | kanglemycin A/kanglemycin V1/kanglemycin V2 | PKS

BGC0002009 - Region 1 - NRPS-like,T1PKS
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
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Location: 1 - 113,783 nt. (total: 113,783 nt) Show pHMM detection rules used
Region on contig edge.
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
MIBiG
Tailoring
MIBiG comparison
KnownClusterBlast
NRPS/PKS modules
NRPS/PKS domains
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
External annotation
This annotation was made externally by 'MIBiG' and not by antiSMASH

Name |  | MIBiG
Version |  | 4.0alpha

Type |  | Subregion
Location |  | 1 - 113,784
Label |  | BGC0002009

Tailoring functions
Tailoring enzymes detected in this region
Oxidoreductases: oxidoreductase oxygenase
OO60_RS14035: cytochrome P450

smcogs
  SMCOG1007: cytochrome P450

OO60_RS14050: cytochrome P450

smcogs
  SMCOG1007: cytochrome P450

OO60_RS14065: monooxygenase FAD-binding

smcogs
  SMCOG1050: monooxygenase FAD-binding

OO60_RS14105: cytochrome P450

smcogs
  SMCOG1007: cytochrome P450

OO60_RS14110: cytochrome P450

smcogs
  SMCOG1007: cytochrome P450

OO60_RS14135: aldo/keto reductase family oxidoreductase

smcogs
  SMCOG1039: aldo/keto reductase family oxidoreductase

OO60_RS14150: cytochrome P450

smcogs
  SMCOG1007: cytochrome P450

OO60_RS14165: oxidoreductase

smcogs
  SMCOG1079: oxidoreductase

OO60_RS14225: cytochrome P450

smcogs
  SMCOG1007: cytochrome P450

OO60_RS14240: oxidoreductase

smcogs
  SMCOG1079: oxidoreductase

Transferases: acylase aminotransferase glycosyltransferase transferase
OO60_RS14100: acetyl-CoA carboxylase, carboxyl transferase,

smcogs
  SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

OO60_RS14120: glycosyltransferase, MGT family

smcogs
  SMCOG1062: glycosyltransferase, MGT family

OO60_RS14170: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs
  SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

OO60_RS14210: malonyl CoA-acyl carrier protein transacylase

smcogs
  SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase hydrolase
OO60_RS14055: thioesterase

smcogs
  SMCOG1004: thioesterase

OO60_RS14160: HAD-superfamily hydrolase, subfamily IA, variant

smcogs
  SMCOG1115: HAD-superfamily hydrolase, subfamily IA, variant

Lyases: dehydratase
OO60_RS14070: L-carnitine dehydratase/bile acid-inducible

smcogs
  SMCOG1189: L-carnitine dehydratase/bile acid-inducible

OO60_RS14140: NAD-dependent epimerase/dehydratase

smcogs
  SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthase
OO60_RS14040: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs
  SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

OO60_RS14185: 3-dehydroquinate synthase

smcogs
  SMCOG1183: 3-dehydroquinate synthase

OO60_RS14200: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

OO60_RS14205: Beta-ketoacyl synthase

smcogs
  SMCOG1093: Beta-ketoacyl synthase

OO60_RS14215: Beta-ketoacyl synthase

smcogs
  SMCOG1093: Beta-ketoacyl synthase

OO60_RS14220: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters
Shows areas that are similar to the current region to a reference database.
Mouseover a score cell in the table to get a breakdown of how the score was calculated.The MIBiG database.
Click on an accession to open that entry in the MIBiG database.
Analysis type:

Reference | T1PKS | NRPS-like | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | --- | ---
BGC0002009.4 |  |  | 1.19 | PKS | kanglemycin A, kanglemycin V1, kanglemycin V2 | Amycolatopsis vancoresmycina
BGC0001273.3 |  |  | 0.90 | PKS | asperlactone | Aspergillus ochraceus
BGC0001244.3 |  |  | 0.90 | PKS | (-)-Mellein | Parastagonospora nodorum
BGC0001164.3 |  |  | 0.72 | PKS | 1-heptadecene | Cyanothece sp. PCC 7822
BGC0001160.5 |  |  | 0.71 | PKS | 1-nonadecene, (14Z)-1,14-nonadecadiene | Synechococcus sp. PCC 7002
BGC0000041.3 |  |  | 0.56 | PKS | oronofacic acid | Pseudomonas syringae
BGC0000067.5 |  |  | 0.53 | PKS | geldanamycin | Streptomyces hygroscopicus subsp. duamyceticus
BGC0001287.5 |  |  | 0.49 | PKS | chaxamycin A, chaxamycin B, chaxamycin C, chaxamycin D | Streptomyces leeuwenhoekii
BGC0000136.5 |  |  | 0.48 | PKS | rifamycin | Amycolatopsis mediterranei S699
BGC0001012.3 |  |  | 0.46 | NRPS, PKS | meridamycin | Streptomyces violaceusniger

Reference | Aggregated | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0002009.4 |  | 1.00 | PKS | kanglemycin A, kanglemycin V1, kanglemycin V2 | Amycolatopsis vancoresmycina
BGC0001276.3 |  | 0.76 | PKS | 6-methylsalicyclic acid | Aspergillus terreus
BGC0001273.3 |  | 0.75 | PKS | asperlactone | Aspergillus ochraceus
BGC0001244.3 |  | 0.75 | PKS | (-)-Mellein | Parastagonospora nodorum
BGC0001275.3 |  | 0.75 | PKS | 6-methylsalicyclic acid | Glarea lozoyensis
BGC0001164.3 |  | 0.69 | PKS | 1-heptadecene | Cyanothece sp. PCC 7822
BGC0001160.5 |  | 0.69 | PKS | 1-nonadecene, (14Z)-1,14-nonadecadiene | Synechococcus sp. PCC 7002
BGC0002154.2 |  | 0.66 | PKS | 1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene | Pyricularia oryzae 70-15
BGC0001284.4 |  | 0.66 | PKS | alternariol | Parastagonospora nodorum SN15
BGC0001257.3 |  | 0.66 | PKS | 1,3,6,8-tetrahydroxynaphthalene | Nodulisporium sp. ATCC74245

Similar known clusters from MIBiG 4.0
Shows clusters from the MIBiG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Detailed help and explanations are available here.
Click on reference genes to show details of similarities to genes within the current region.
Double click on a reference drawing to reverse the display of the genes.
Click on an accession to open that entry in the MIBiG database.
Location:
Identifier
Identity
Coverage
Module view
Shows module structures for each candidate cluster in NRPS and PKS regions.
Genes are shown in predicted order, and are only present when containing at least one complete module.
A domain glossary is available here, and an explanation of the visualisation is available here.
Legend
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A domain glossary is available here, and an explanation of the visualisation is available here.
Selected features only
Show module domains
NRPS/PKS products
NRPS/PKS substrates
Predicted core structure(s)
Shows estimated product structure and polymer for each candidate cluster in the region. To show the product, click on the expander or the candidate cluster feature drawn in the overview.
For candidate cluster 1, location 22994 - 113783:
Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:
  (AHBA - Me-ccmal - mal - Me-ohmal) + (Me-ccmal - Me-ccmal - Me-ccmal) + (Me-ccmal) + (pk) + (ccmal - Me-ccmal)
Non-colinear ordering:
  OO60_RS14220 + OO60_RS14215 + OO60_RS14210 + OO60_RS14205 + OO60_RS14200

Direct lookup in NORINE database: strict or relaxed
Link to NORINE database query form
NRPS/PKS substrate predictions
Shows the predicted substrates for each adenylation domain and acyltransferase within genes. Each gene prediction can be expanded to view detailed predictions of each domain. Each prediction can be expanded to view the predictions by tool (and, for some tools, further expanded for extra details).
OO60_RS14200: mal - mmal
PKS_AT (574..861): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 79.2%
  2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 188.2
  inactive: 109.6
  Methoxymalonyl-CoA: 59.6
  Methylmalonyl-CoA: 46.4
  Isobutyryl-CoA: 35.3
  Propionyl-CoA: 29.7
  fatty_acid: 26.2
  Acetyl-CoA: 23.9
  Ethylmalonyl-CoA: 23.2
  Benzoyl-CoA: 18.0
  2-Methylbutyryl-CoA: 15.3
  trans-1,2-CPDA: 13.7
  CHC-CoA: 11.5
  3-Methylbutyryl-CoA: 0.0

PKS_AT (2280..2570): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 91.7%
  Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 210.8
  Ethylmalonyl-CoA: 195.8
  Methoxymalonyl-CoA: 166.9
  trans-1,2-CPDA: 113.4
  2-Methylbutyryl-CoA: 102.6
  Propionyl-CoA: 88.0
  Malonyl-CoA: 87.5
  CHC-CoA: 85.3
  Isobutyryl-CoA: 75.5
  Benzoyl-CoA: 69.6
  Acetyl-CoA: 44.1
  fatty_acid: 26.8
  inactive: 19.4
  3-Methylbutyryl-CoA: 17.5

OO60_RS14205: pk
PKS_AT (530..819): pk
ATSignature: Ethylmalonyl-CoA

Top 3 matches:
  Ethylmalonyl-CoA: 91.7%
  Methoxymalonyl-CoA: 87.5%
  Methylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 190.2
  Ethylmalonyl-CoA: 185.9
  Methoxymalonyl-CoA: 162.0
  trans-1,2-CPDA: 109.3
  2-Methylbutyryl-CoA: 107.4
  CHC-CoA: 99.4
  Isobutyryl-CoA: 97.7
  Propionyl-CoA: 93.2
  Malonyl-CoA: 82.2
  Benzoyl-CoA: 71.6
  Acetyl-CoA: 50.5
  3-Methylbutyryl-CoA: 40.0
  fatty_acid: 31.3
  inactive: 28.3

OO60_RS14210: mmal
PKS_AT (568..858): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 91.7%
  Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 211.4
  Ethylmalonyl-CoA: 207.2
  Methoxymalonyl-CoA: 169.4
  2-Methylbutyryl-CoA: 112.5
  trans-1,2-CPDA: 111.8
  Propionyl-CoA: 100.7
  Malonyl-CoA: 97.7
  CHC-CoA: 91.3
  Isobutyryl-CoA: 82.6
  Benzoyl-CoA: 61.1
  Acetyl-CoA: 49.3
  fatty_acid: 39.7
  inactive: 30.1
  3-Methylbutyryl-CoA: 24.3

OO60_RS14215: mmal - mmal - mmal
PKS_AT (568..862): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 91.7%
  Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 216.5
  Ethylmalonyl-CoA: 202.4
  Methoxymalonyl-CoA: 175.7
  2-Methylbutyryl-CoA: 123.2
  trans-1,2-CPDA: 119.0
  Malonyl-CoA: 100.8
  Propionyl-CoA: 100.7
  CHC-CoA: 96.1
  Isobutyryl-CoA: 94.5
  Benzoyl-CoA: 62.2
  Acetyl-CoA: 35.4
  fatty_acid: 20.6
  3-Methylbutyryl-CoA: 20.4
  inactive: 16.7

PKS_AT (2240..2528): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 91.7%
  Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 209.0
  Ethylmalonyl-CoA: 207.5
  Methoxymalonyl-CoA: 169.9
  2-Methylbutyryl-CoA: 132.8
  trans-1,2-CPDA: 112.2
  Propionyl-CoA: 105.2
  Malonyl-CoA: 93.3
  CHC-CoA: 86.3
  Isobutyryl-CoA: 74.2
  Benzoyl-CoA: 53.6
  Acetyl-CoA: 44.4
  fatty_acid: 34.0
  inactive: 24.6
  3-Methylbutyryl-CoA: 21.2

PKS_AT (3853..4146): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 87.5%
  Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 211.0
  Ethylmalonyl-CoA: 204.5
  Methoxymalonyl-CoA: 162.4
  2-Methylbutyryl-CoA: 118.1
  trans-1,2-CPDA: 106.9
  Propionyl-CoA: 94.9
  Isobutyryl-CoA: 82.4
  Malonyl-CoA: 81.4
  CHC-CoA: 78.1
  Benzoyl-CoA: 56.6
  Acetyl-CoA: 49.3
  fatty_acid: 28.2
  inactive: 23.2
  3-Methylbutyryl-CoA: 15.0

OO60_RS14220: AHBA - mmal - mal - mmal
CAL_domain (33..436): AHBA
minowa: AHBA

Prediction, score:
  AHBA: 483.7
  shikimic_acid: 236.9
  Acetyl-CoA: 19.9
  fatty_acid: 0.0
  NH2: 0.0

PKS_AT (1158..1445): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 91.7%
  Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 212.3
  Ethylmalonyl-CoA: 205.2
  Methoxymalonyl-CoA: 171.4
  2-Methylbutyryl-CoA: 121.7
  trans-1,2-CPDA: 121.4
  Propionyl-CoA: 105.6
  Malonyl-CoA: 100.4
  CHC-CoA: 97.0
  Isobutyryl-CoA: 91.9
  Benzoyl-CoA: 61.5
  fatty_acid: 48.7
  Acetyl-CoA: 48.4
  3-Methylbutyryl-CoA: 34.9
  inactive: 21.8

PKS_AT (2725..3013): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 100.0%
  inactive: 79.2%
  Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 210.2
  inactive: 111.6
  Methoxymalonyl-CoA: 69.7
  Methylmalonyl-CoA: 58.7
  Propionyl-CoA: 40.6
  Isobutyryl-CoA: 37.3
  Benzoyl-CoA: 20.1
  Ethylmalonyl-CoA: 19.5
  2-Methylbutyryl-CoA: 18.0
  Acetyl-CoA: 17.3
  CHC-CoA: 16.5
  trans-1,2-CPDA: 10.4
  fatty_acid: 0.0
  3-Methylbutyryl-CoA: 0.0

PKS_AT (3714..4006): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 91.7%
  Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 208.7
  Ethylmalonyl-CoA: 197.0
  Methoxymalonyl-CoA: 168.8
  2-Methylbutyryl-CoA: 138.9
  trans-1,2-CPDA: 107.4
  Malonyl-CoA: 106.9
  Propionyl-CoA: 102.7
  CHC-CoA: 88.7
  Isobutyryl-CoA: 88.1
  Benzoyl-CoA: 53.5
  Acetyl-CoA: 32.2
  inactive: 26.3
  fatty_acid: 23.8
  3-Methylbutyryl-CoA: 14.3

Link to PARAS substrate predictor