LOCUS       BGC0002015             12018 bp    DNA     linear   CON 08-SEP-2022
DEFINITION  Streptomyces sp. ZS0098 contig44, whole genome shotgun sequence.
ACCESSION   BGC0002015
VERSION     BGC0002015
KEYWORDS    .
SOURCE      Streptomyces sp. ZS0098
  ORGANISM  Streptomyces sp. ZS0098
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            MKCP01000039.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 16:43:18
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 18548
            Orig. end    :: 30566
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..702)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016:LuxR family
                     DNA-binding response regulator (Score: 121.3; E-value:
                     5.6e-37)"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /locus_tag="BIU87_RS24165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BIU87_31850"
                     /product="response regulator transcription factor"
                     /protein_id="WP_147474396.1"
                     /transl_table=11
                     /translation="MTVNLFVVSDNAVSLAGLEAIVDQDEECALVGRATDDSDDLRCLS
                     DLAGTALHPDVVVLSEPSSSRTVSARVRHIASAFPDEASRPHTIVVAQNDDDEMIVSAL
                     RAGVNGYLSRLGSADELLRSLKVVARGGAAFCPSVAARMSRYFSAMQGLHDGAGFPGLT
                     SRELEILELLAGGMGNRQIARRLFLAEKTVRNYVSRIFAKLEVHDRATAVVMARDAGFG
                     EPAGELVRNGA"
     subregion       1..12018
                     /aStool="mibig"
                     /contig_edge="False"
                     /label="ribosomal"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12018
                     /contig_edge="False"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1..702)
                     /locus_tag="BIU87_RS24165"
                     /old_locus_tag="BIU87_31850"
     gene            1184..2323
                     /locus_tag="BIU87_RS24170"
                     /old_locus_tag="BIU87_31855"
     CDS             1184..2323
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048:sensor
                     histidine kinase (Score: 214.8; E-value: 3.6e-65)"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018958865.1"
                     /locus_tag="BIU87_RS24170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BIU87_31855"
                     /product="sensor histidine kinase"
                     /protein_id="WP_122215938.1"
                     /transl_table=11
                     /translation="MGEGSRRRAIVLDAAAACAFTLLPQVSLLRPAVDGGTGGGFLWLL
                     SLAAGLPLAVRRLWPVPVFVLVLAAACGALAAGLGPASFLAAAYAVHTVATTRRRDPGV
                     SAVAVAGLCAAVAALLTLSGGQSYQGGSTAVQAVFGLLVLGATWAAGSAVRERRRSTRR
                     AIEHAAEQAKTEERLRIARDIHDVVTHSVGLIAVKAGIANHVVATRPEEAKEALAVIED
                     VSRRALRDMRATLTVLRGEDRSEAGDLRPARGLADLPALLETAEAAGVRVELRTRYDQE
                     PPEGVALAAFRIVQESLTNVLKHAAPTGCRVDVTARQGVLTVDVTDDGPGPGRRTTVPG
                     GGMGLVGMKERAAAHGGTLAAGPRPGGGFRVTATLPFQA"
     gene            2434..3336
                     /locus_tag="BIU87_RS24175"
                     /old_locus_tag="BIU87_31860"
     CDS             2434..3336
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 166.9; E-value:
                     9.9e-51)"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006136293.1"
                     /locus_tag="BIU87_RS24175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BIU87_31860"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_183153327.1"
                     /transl_table=11
                     /translation="MLAERLTKRYGPATVVDDLSFTVRPGVVTGFLGPNGAGKSTTMRM
                     MLGLTRPDGGTATVGGRAYRDLTYPLRHVGALLETSAPHRGMTAVGHLSWLARSNRVPR
                     RRVDEALDAVGLTEAARKRVGTFSLGMGQRLGLAAALLGDPPVLVLDEPVNGLDAEGIR
                     WLRELLRAKAAEGRTILVSSHLMAEMAQVADELIVISRGRLLAETSVSEFLGRHGRTFV
                     RVRTSEPLRAAQEFQAKGATSVRRAADGGLEVDGLPAGEVNRIAAAAGVPVEELSTHTG
                     SLEETFLKLVDDGGDPTHV"
     gene            3329..4081
                     /locus_tag="BIU87_RS24180"
                     /old_locus_tag="BIU87_31865"
     CDS             3329..4081
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /locus_tag="BIU87_RS24180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BIU87_31865"
                     /product="ABC transporter permease"
                     /protein_id="WP_102640375.1"
                     /transl_table=11
                     /translation="MSETLAAARAEFTKMRAVRATSVALLLFVAVSVFIAALGGWSAKG
                     AIESGNPGLRSDFTPEQAGLDGILYGQLALIVFGVLMMSGEYTSGMMRVSLLAVPRRGR
                     LYLAKTAVTAVAALAVALPVTVVSYLVTQLALGPHGSTLDADGVPRALAGAVVYLTLMS
                     LLAVGVAAAARSAVLPLAVLLPLVLVGSQILSVIGATKEVARWFPDRAGAQMLTVDSGD
                     ALTGLVVLLAWTAAALTAGWLRHRSWDG"
     gene            4198..4326
                     /locus_tag="BIU87_RS24185"
     CDS             4198..4326
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_103079659.1"
                     /locus_tag="BIU87_RS24185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aborycin family tricyclic lasso peptide"
                     /protein_id="WP_106963308.1"
                     /transl_table=11
                     /translation="MTAIYEPPALQEIGDFDELTKCLGIGSCNDFAGCGYAVVCFW"
     gene            4388..6199
                     /locus_tag="BIU87_RS24190"
                     /old_locus_tag="BIU87_31870"
     CDS             4388..6199
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177:asparagine synthase (glutamine-hydrolyzing)
                     (Score: 124.1; E-value: 1.1e-37)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_067372303.1"
                     /locus_tag="BIU87_RS24190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BIU87_31870"
                     /product="lasso peptide isopeptide bond-forming cyclase"
                     /protein_id="WP_122215940.1"
                     /sec_met_domain="Asn_synthase (E-value: 1.6e-40, bitscore:
                     129.7, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFTVLPDCPAGAALADRVAAPKRIDHASGRPWIVGDWPEGGTTV
                     TEAGTRRLAVFGHTRPDDAGTASALGRMRSLHDVDRVASRLPGVFHLAASLDGAVRLQG
                     SVAGVRQVFTARVDGVTVAASAVDPLLRLTGAGLDETLLAARLLAPGGAPWPLSPRPVR
                     RGVDALPTGHWLELGADGRARSVRWWELPEATLSLEEGAGAVRSALTDALATRVDPHRT
                     ISADLSGGLDSTTLCFLADAAGADLVTYHVVPLDEANEDTAWARKAAAHLPHARHHMLA
                     ADRAANLFDIGYTADTVNAAPEGPSTWASGLAHIRDLAGRATAEGAALHLSGFGGDELF
                     GRMPACAWSLARLRPADGLRLVNRYRLANRWPWRSTLRQLADRSTFAQNLAEVAGRITA
                     PPPPVNEPDFGWVFAPRMPAWATPDAVAAVRDLLTTAAERTPEPLDADRARHQALSSIV
                     FEGNTVRQVNTAVAGTGLVWEAPFLDDRVLEAAFTTRIDERLAAGRFKPLLTTAVRGLV
                     PDDFLARRDKGEFSAETFRGIERNRDRILDLCEDSLLARLGLVDPHAFRAAVLNPGPMS
                     HHLQPIQTTVACESWLRAHPQDTGENR"
     gene            6196..6459
                     /locus_tag="BIU87_RS24195"
                     /old_locus_tag="BIU87_31875"
     CDS             6196..6459
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lasso_Fused_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF033530.1"
                     /locus_tag="BIU87_RS24195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BIU87_31875"
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_037640857.1"
                     /sec_met_domain="Lasso_Fused_RRE (E-value: 2.2e-09,
                     bitscore: 27.3, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 1e-24,
                     bitscore: 76.4, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 1.4e-14, bitscore: 44.2,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLSLARDVTLTPVDSGAVLLDGRRGRYYQLNASGSAILHKLLDG
                     DTPAAAAASLSESAPVSEERVHQDVLALVRSLSEADLVEVTQ"
     gene            6456..6881
                     /locus_tag="BIU87_RS24200"
                     /old_locus_tag="BIU87_31880"
     CDS             6456..6881
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020123203.1"
                     /locus_tag="BIU87_RS24200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BIU87_31880"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_122215941.1"
                     /sec_met_domain="PF13471 (E-value: 1.2e-27, bitscore: 86.2,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPAVAEQATRLPLHRQIAPRCAAGAARLLVRLPPARLHRVLRV
                     LSKGSRPAGYGQVARARRSVVSVSTRCAGLGCLQRSVATVLLLRVRGRWADWCTGFRVQ
                     PFAAHAWVEAGGRPVDEPGEVGVFRTVLAVRRTGGGS"
     gene            6878..7843
                     /locus_tag="BIU87_RS24205"
                     /old_locus_tag="BIU87_31885"
     CDS             6878..7843
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 198.6; E-value:
                     2e-60)"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007385816.1"
                     /locus_tag="BIU87_RS24205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BIU87_31885"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_122215942.1"
                     /transl_table=11
                     /translation="MTAIRAEGLYAYYGTTPAVNGLHLNVPEGATFGFLGPNGAGKTTT
                     ISMLTTLLRPTAGRAEVAGFDVTTHAAEVRRRIGIVFQESTLDLELTATENLRFQADLC
                     GLGRAEARAAVAAMLDMMELTGRDRTPVRHFSTGLRRRLEIARGLLGSPRVLFLDEPTT
                     GLDTQTRAAVWHHLDRLREEQGITVFFTTHQLEEAEHCDRIAIFDRGKLVTEGSPAELK
                     SVIGADVVDLRTEDDRLAVDLLSDRFGLTAENTPGGLRLRVQDGASMVPRLCTGLGLGV
                     RSVTVTPPSLDDVYLHHTGTAIRDSGSDARSLDSLGEGLR"
     gene            7840..8709
                     /locus_tag="BIU87_RS24210"
                     /old_locus_tag="BIU87_31890"
     CDS             7840..8709
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1065:ABC-2 type
                     transporter (Score: 129.4; E-value: 2.1e-39)"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020123205.1"
                     /locus_tag="BIU87_RS24210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BIU87_31890"
                     /product="ABC transporter permease"
                     /protein_id="WP_122215943.1"
                     /transl_table=11
                     /translation="MTRTDIPPSVTVTKPAPPSSEGRTRHTVRPYALLWRREMTRLRHN
                     PLRLVMGLVTPLLFLVVLGTGLEAASSTLGKAQLNDYRAYLFPGALVMSVQAPAIAVGI
                     SLVWDRRLGMLRQMLVSPFPRSSIVLGLALGGATTGAVYGLALLAVGGIAGVRYTPMLL
                     VVLVELLLVSLLFTSFGLLAAVTIRQVDTFQIVVNLSLMPLMFFSGAMFPPNGLPGWLD
                     TVVKLNPLTYGVDAVRRTLPGPDVLTSEQTRLVLGDWNPPVYAELGVLAALTAAVLGLA
                     TYRFSRTQ"
     gene            8723..9196
                     /locus_tag="BIU87_RS24215"
                     /old_locus_tag="BIU87_31895"
     CDS             8723..9196
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /locus_tag="BIU87_RS24215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BIU87_31895"
                     /product="DoxX family membrane protein"
                     /protein_id="WP_122215944.1"
                     /transl_table=11
                     /translation="MTFVQTWAGTAARIVLAAVLGYAGWVKVQDLTGAGRTVALYQLVP
                     EESAQLVGAAVAFVELALALLLLAGLATRAVAAVTALLMVTYIAAIASVWARGMSIDCG
                     CFSSGGTLTGGAERGYVVDIARDLAFLGAAAFLITRPRTRYALDRLVLETKER"
     gene            9199..9924
                     /locus_tag="BIU87_RS24220"
                     /old_locus_tag="BIU87_31900"
     CDS             9199..9924
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /locus_tag="BIU87_RS24220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BIU87_31900"
                     /product="thioredoxin domain-containing protein"
                     /protein_id="WP_122215945.1"
                     /transl_table=11
                     /translation="MQPSTDAQVREMVHRRRRRRRTVLVSLAAVVTVLGAALVGAGLVR
                     ATDTEPGDAPEEVPAGVAADRAGLVTSEGPVRVDLYLDYLCPECRITEKALAPELREMQ
                     ERGEVRVVHHPVAFLDDRSAPAGYSTRAASAAACAADRNKFEPYTAALFDEQPPEQGPG
                     LDTDRLVALGRDVGITGAAFERCVRDGTYRPWVTYVSDVAASRGVALTPTVKVAGKRVE
                     LSGPDRAKAFVRAVEESRS"
     gene            9921..11267
                     /gene="mslH"
                     /locus_tag="BIU87_RS24225"
                     /old_locus_tag="BIU87_31905"
     CDS             9921..11267
                     /codon_start=1
                     /gene="mslH"
                     /inference="COORDINATES: protein motif:HMM:NF038314.1"
                     /locus_tag="BIU87_RS24225"
                     /note="Involved in the biosynthesis of MS-271 and related
                     lasso peptides; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /old_locus_tag="BIU87_31905"
                     /product="lasso peptide C-terminal Trp epimerase"
                     /protein_id="WP_122215946.1"
                     /transl_table=11
                     /translation="MSRLTVALTGDCMMTRGAPVTSDPAAGRLGEVLHTADFAFTNLEV
                     APATGRGHPVPDAASGGGLIADPAVLDDVTAMGFSVLSCANNHALDLGTEGVLGTVDLL
                     RAKGVPFAGIGADLTAARRPVYVDRPAGSLALIACTATFRDGQEAAAPSPDLPGRPGLS
                     PLRHGVTLRVTAAQLEALREIDGATGLRSRRAEARTLLGFDPAMPGPGRLALFGTRFET
                     ADTPGLTTACDPRDVHDLTRWVAEARRRADLVLVSVHSHEPGPTPETPAEFLRDFARAV
                     IDAGADAVVGHGPHMLRGVELHRGRPVFHSLGNIVSQIELADRIPAEDYARAGTGEALT
                     PGGYFERITAHGRRMFAPHRRYWQSLVPVLTFDDGVFAGARLHPVDLGFGQPVHRRGRP
                     RLAGPVDAKEILTDFARLAEPHGVTVTVGDDGTGELVAAETDGGHRPGR"
     gene            11341..12018
                     /locus_tag="BIU87_RS24230"
                     /old_locus_tag="BIU87_31910"
     CDS             11341..12018
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016:LuxR family
                     DNA-binding response regulator (Score: 195.5; E-value:
                     1.1e-59)"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020123207.1"
                     /locus_tag="BIU87_RS24230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BIU87_31910"
                     /product="response regulator transcription factor"
                     /protein_id="WP_122215947.1"
                     /transl_table=11
                     /translation="MAEAVRVLIADDQALLRGSFRVLVDSTPGMETVGEAGDGAEAVAL
                     ARELTPDVVLMDLRMPVMDGIAATREICSDASMAGVRVLALTMFDMDEYVYPALQAGAS
                     GFMLKDTSPDDLVAGIRVIATGEGVLAPSVTRRVIANFSHRDTAARPAPRLVGLTRREQ
                     EVLVLIAKGLSNAEIADRLVISLPTVKTHVSSLLSKLNARDRAQLVIVAYESGIAERGM
                     PPA"
ORIGIN
        1 tcacgccccg ttccggacca gttccccggc gggctcgccg aagcccgcgt cccgggccat
       61 caccaccgcc gtcgcccggt cgtgcacctc gagcttggcg aagatgcgcg agacgtagtt
      121 gcggacggtc ttctcggcca ggaagagccg gcgggcgatc tgccggttgc ccatgccgcc
      181 ggccaggagc tccagtatct ccagctcgcg gctggtgagc cccgggaacc cggcgccgtc
      241 gtgcaggccc tgcatggcgg agaagtagcg gctcatgcgg gccgcgacgc tggggcagaa
      301 ggcggcgccg ccccgggcga cgaccttcag ggagcggagc agttcgtcgg ccgaaccgag
      361 gcgggagagg tagccgttga cacccgcgcg gagggccgag acgatcatct cgtcgtcgtc
      421 gttctgcgcc accacgatcg tgtgcgggcg ggacgcctcg tcggggaagg cggaggcgat
      481 gtgccggacg cgtgcggaga cggttctgct ggaggacggt tcgctgagga cgaccacgtc
      541 ggggtggagg gcggtgccgg ccaggtccga cagacatcgg aggtcgtccg agtcgtccgt
      601 ggcgcggccg acaagagcgc actcctcatc ctgatcgaca atcgcttcca gcccggcgag
      661 ggacaccgcg ttgtcgctga ccacgaacag gttgacggtc atctcgtaca tcccccagaa
      721 atgcgtcggc gtggtgcgga cggcgccgga cggtccccgg gggcacacgc gacgaagggt
      781 cggtccgtag gcgaccgact ccgggcgtga cgtggtcagt tgtggttttc agcggccccc
      841 gtggccatga cctgtcggcg caggcatggt ggtgcatcga aagcgcgcaa ggcgctcgtg
      901 atgccgagta tgtggacgtg tccggagaga acgcaaggtc gccaacgccc tcgtattgtc
      961 cgaggggtga gtatcggccg gaccccgccg aaccaccgcg attccggcgt cggcggtggt
     1021 gggggcggct ccgggcgcgc gacccgcagg accgcgcgcc cgtgtgggcc gtggtgcggg
     1081 gcggacgaca ctgtccgaaa cggtcaccgc ccggcgcgac acgcggacgt ccggccctgc
     1141 gcccgttgga cgggccgtgg agcgagcgct tacgttcgac ggcatgggtg aggggtcccg
     1201 gaggcgtgcg atcgtgctgg acgcggcggc ggcctgcgcg ttcacgctcc tgccgcaggt
     1261 gtcgctgctg cggccggccg tcgacggcgg gacgggcggc ggcttcctgt ggctgctgtc
     1321 cctggccgcc ggactcccgc tcgccgtaag gcggttgtgg cccgtcccgg tcttcgtcct
     1381 cgtcctggcc gccgcctgcg gggcgctggc cgccggcctc ggacccgcct cgttcctggc
     1441 cgccgcctac gccgtccaca cggtggccac cacccggcgg cgcgaccccg gggtgtcggc
     1501 cgtcgccgtt gccgggctgt gcgcggcggt cgccgccctc ctcacgctgt cgggcgggca
     1561 gtcctaccag ggcggcagca ccgccgtgca ggccgtgttc ggcctcctcg tgctcggcgc
     1621 gacctgggcg gccggctcgg cggtcaggga acgcagacgc agtacccggc gcgccatcga
     1681 gcacgccgcc gagcaggcga agaccgagga gcgcctgcgt atcgcccgcg acatccacga
     1741 cgtcgtcacc cacagcgtgg gactcatcgc cgtcaaggcc ggcatcgcca accacgtcgt
     1801 ggccacccgc cccgaggagg cgaaggaggc cctggcggtc atcgaggacg tcagccgccg
     1861 ggcgctgcgc gacatgcgcg ccaccctgac cgtgctgcgc ggtgaggacc ggagcgaggc
     1921 cggggatctg cggcccgccc gcggccttgc ggacctgccc gcgctgctcg agaccgccga
     1981 ggcggcaggc gtccgcgtcg aactgcgcac ccgctacgac caggaaccgc ccgagggtgt
     2041 cgcgctggcg gcgttccgca tcgtccagga gtccctcacc aacgtgctca agcacgccgc
     2101 gccgaccggc tgccgggtgg acgtcacggc gcggcagggc gtgctgacgg tggacgtgac
     2161 cgacgacggc cccggccccg gacgccggac cacggtgccc ggcggcggga tggggctggt
     2221 cggcatgaag gagcgggccg ccgcgcacgg cggcaccctc gccgccggtc cccggcccgg
     2281 cggcggcttc cgggtgacgg ccaccctgcc gttccaggcc tgacggacac cggctcatcc
     2341 tcgggtatga ggggggcccg cgcggcaccg cgccgggttc ccgaccgcgg accgatgcgg
     2401 tcgcggggtg cgcgccgcga gactcgacac gtgatgcttg ccgagagact gaccaagcgg
     2461 tacgggcccg ccaccgtcgt cgacgacctg tccttcacgg tgcgaccagg tgtcgtcacc
     2521 ggcttcctcg ggcccaacgg cgccggaaag tccacgacga tgcggatgat gctcggcctg
     2581 acccgccccg acggcggcac ggccaccgtc ggcggacgcg cctaccgcga cctgacctac
     2641 ccgctccggc acgtcggcgc gctcctggag acgtcggccc cgcaccgtgg catgaccgcc
     2701 gtcggccatc tgtcgtggct ggcgcgcagc aaccgcgttc cccgccggcg ggtggacgag
     2761 gcgctcgacg cggtcggtct cacggaagcc gcccgtaaac gggtcggcac cttctccctg
     2821 ggcatgggac agcgcctcgg cctggcggct gccctcctcg gcgacccccc ggtcctggtg
     2881 ctcgacgagc cggtcaacgg gctcgacgcc gagggcatcc ggtggctgcg cgaactgctg
     2941 cgcgccaagg cagccgaggg ccgcaccatc ctcgtctcca gccatctgat ggcggagatg
     3001 gcgcaggtcg ccgacgagct gatcgtgatc agccggggcc ggctcctcgc cgagaccagc
     3061 gtgtcggagt tcctcggacg ccacggccgg acgttcgtac gggtccggac ctccgaaccg
     3121 ctgcgggcgg cgcaggagtt ccaggcgaag ggcgccacct cggtgcggcg ggccgcggac
     3181 ggcggcctgg aggtggacgg tctgccggcg ggcgaggtga accgcatcgc cgcggcggcc
     3241 ggtgtccccg tcgaggaact gagcacccac accggctcgc tggaggagac cttcctcaag
     3301 ctcgtcgacg acggaggaga tcccacgcat gtctgagacc ctcgcggccg cgcgggcgga
     3361 gttcaccaag atgcgggcgg tgcgcgccac gtccgtcgca ctgctgctgt tcgtcgccgt
     3421 cagcgtgttc atcgccgcac tgggcggctg gtccgccaag ggcgcgatcg agtccggcaa
     3481 tcccgggctg cgctccgact tcacgcccga gcaggccggc ctggacggca tcctctacgg
     3541 ccagctcgcc ctgatcgtgt tcggggtgct gatgatgtcc ggcgagtaca cctcgggcat
     3601 gatgcgggtc tcgctgctcg cggtgccccg gcggggccgg ctctacctgg ccaagacggc
     3661 cgtcaccgcc gtcgcggccc tggccgtcgc ccttccggtc acggtcgtgt cgtacctggt
     3721 cacccaactc gccctgggcc cccacgggtc gacgctcgac gcggacggcg tcccgcgcgc
     3781 cctggccggc gcggtcgtct acctgaccct catgagcctg ctcgccgtag gggtggcggc
     3841 cgccgcccgc agtgccgtcc tcccgctggc cgtgctgctg ccgctggtgc tggtcggctc
     3901 gcagatcctg tccgtcatcg gggcgaccaa ggaggtggcg cgctggttcc ccgaccgggc
     3961 cggcgcccag atgctcaccg tcgactccgg cgacgccctc accggcctcg tcgtgctgct
     4021 cgcgtggacc gcggccgccc tgacggccgg ctggctccgg caccgttcgt gggacgggtg
     4081 acgggacgcg cctgagtcat gtgccccatg cacgtgggac atggcccctc gtaggttccc
     4141 ggacaggtca cggggccgaa aaggtcggcc cgggccgttc gacccacggg aggaaccatg
     4201 accgcgatct acgagccgcc cgccctgcag gagatcggcg acttcgacga gctcaccaag
     4261 tgcctcggca tcgggagctg caacgacttc gccggctgcg gttacgccgt cgtctgcttc
     4321 tggtgatcgc accggtgccg gtgtgcccct cgtggcacac cggcaccgcc cgggggagtg
     4381 aggcgacatg gaattcacag tgcttccgga ctgtcccgcc ggcgccgcgc tggcggaccg
     4441 ggtggcggca ccgaagcgga tcgaccacgc gtcgggacgg ccctggatcg tgggggactg
     4501 gcccgagggc gggacgacgg tgaccgaggc cggcacccgc cgcctggcgg tgttcggaca
     4561 cacccggccc gacgacgcgg gaacggcctc cgcgctcggc cggatgcgct cgctgcacga
     4621 cgtcgaccgg gtcgcgtccc ggctgcccgg ggtcttccac ctcgcggcct ccctggacgg
     4681 cgcggtccgc ctccagggct ccgtggccgg cgtacggcag gtcttcaccg cccgggtcga
     4741 cggggtgacg gtcgccgcga gcgccgtgga cccgttgctg cgcctcaccg gcgcggggct
     4801 cgacgagacc ctgctggccg cacggctgct ggcgcccggc ggggcgccct ggccgctctc
     4861 gccgcgcccg gtccgccgcg gcgtggacgc gctgcccacc ggccactggc tggagctcgg
     4921 cgccgacggc cgggcccgca gcgtccggtg gtgggaactg cccgaggcga ccctctcgct
     4981 ggaggagggt gccggggccg tccgttccgc gctcaccgac gccctcgcga cccgtgtgga
     5041 cccgcaccgc acgatcagcg cggacctctc cggcggactc gactcgacca ccctgtgctt
     5101 cctcgccgac gcggccggcg ccgacctggt cacctaccac gtcgtgccgc tcgacgaggc
     5161 caacgaggac accgcatggg cccgcaaggc cgccgcgcat ctgccgcacg cccgccacca
     5221 catgctcgcc gcggaccgcg ccgccaacct gttcgacatc ggctacaccg ccgacaccgt
     5281 caacgcggcc cccgagggcc cctcgacgtg ggcctccggc ctcgcgcaca tccgggacct
     5341 cgcggggcgg gccaccgccg agggggcggc cctgcacctt tccgggttcg gcggggacga
     5401 actcttcggc cggatgcccg cctgcgcctg gtccctcgcc cgcctccgcc cggccgacgg
     5461 cctgcggctg gtgaaccgct accggctggc caaccgctgg ccctggcgca gcaccctgcg
     5521 gcagctcgcg gaccgttcga cgttcgcgca gaacctggcc gaggtcgccg ggcggatcac
     5581 cgccccgccc ccgccggtca acgagcccga cttcggctgg gtgttcgcgc cgcggatgcc
     5641 cgcctgggcc accccggacg cggtggccgc cgtgcgggac ctgctgacca cggcagccga
     5701 acggaccccg gaaccgctgg acgccgaccg ggcccggcac caggcgctgt cctccatcgt
     5761 gttcgagggg aacaccgtcc ggcaggtcaa caccgccgtc gccggcaccg gcctggtctg
     5821 ggaggcgccg ttcctcgacg accgggttct cgaggcggcc ttcaccaccc ggatcgacga
     5881 gcggctggcc gccggacggt tcaagccgct gctcaccacc gccgtgcggg gactcgtgcc
     5941 cgacgacttc ctcgcccgtc gcgacaaggg tgagttcagc gccgagacgt tccggggcat
     6001 cgaacgcaac cgggaccgga tcctggacct ctgcgaggac tcgctcctgg cccggctcgg
     6061 cctcgtcgac ccgcacgcct tccgggccgc ggtgctcaac cccgggccga tgtcccatca
     6121 cctccagccg atccagacca cggtggcgtg cgagagctgg ctgcgggcgc acccccagga
     6181 cacgggagag aaccgatgaa actgagtctt gcccgcgacg tcaccctcac gcccgtcgat
     6241 tccggggcgg tcctgctcga cggccgccgc ggccgctact accagctgaa cgcctccggc
     6301 tccgcgatcc tgcacaagct gctcgacggc gacactcccg ccgcggccgc cgcgagcctg
     6361 tcggagtccg cccccgtcag tgaggagcgg gtgcaccagg acgtgctggc cctggtccgc
     6421 tcgctgagcg aggccgacct cgtggaggtg acacagtgac cacgcccgcc gtcgccgaac
     6481 aggccacgcg cctcccgctg caccggcaga tcgcccccag atgcgcggcc ggcgccgccc
     6541 gcctgctggt caggctgccc ccggcccggc tgcaccgggt gctgcgcgtc ctgagcaagg
     6601 gttcccggcc cgccgggtac ggccaggtgg cgcgggcccg gcggtccgtc gtctcggtca
     6661 gcacccgctg cgcgggcctc ggctgcctcc agcggtccgt cgccaccgtg ctgctgctcc
     6721 gcgtccgggg caggtgggcc gactggtgca ccggcttccg ggtgcagccc ttcgccgcgc
     6781 acgcctgggt cgaggccggc ggccgcccgg tcgacgagcc cggagaggtc ggcgtgttcc
     6841 gcaccgtgct ggccgtgcgc cgcaccggag gcggctcatg acggcgatcc gggccgaggg
     6901 cctctacgcg tactacggca ccacaccggc cgtgaacggc ctccacctga acgtccccga
     6961 gggggccacc ttcgggttcc tggggccgaa cggcgccggc aagaccacca ccatcagcat
     7021 gctcaccacg ctgctcaggc ccacggcggg ccgcgcggag gtggccgggt tcgacgtcac
     7081 cacgcacgcc gccgaggtgc ggcgcaggat cggcatcgtc ttccaggagt cgacgctcga
     7141 cctggagctc acggccaccg agaacctgcg cttccaggcc gacctgtgcg gtctgggccg
     7201 ggccgaggcg cgcgccgcgg tggccgcgat gctcgacatg atggagctga cgggacgcga
     7261 caggaccccc gtgcggcact tctccaccgg cctgcgccgg cgcctggaga tcgcccgcgg
     7321 cctgctcggc tccccgcgcg tgctcttcct cgacgagccg accaccggcc tcgacacgca
     7381 gacccgtgcg gccgtctggc accacctgga ccggctccgc gaggagcagg gcatcacggt
     7441 cttcttcacc acgcaccagc tggaggaggc cgagcactgc gaccgcatcg cgatcttcga
     7501 ccgcggcaag ctggtcacgg agggctcacc cgcggagctg aagtccgtca tcggggccga
     7561 cgtcgtcgac ctgcgcaccg aggacgaccg gctggccgtg gacctgctgt ccgaccgctt
     7621 cgggctgacc gccgagaaca cccccggcgg gctgcggctg agggtgcagg acggcgcctc
     7681 catggtgcct cgcctgtgca ccgggctggg cctgggcgtg cggtcggtga ccgtcacccc
     7741 gccctcgctc gacgacgtct acctgcacca caccgggacc gcgatcaggg acagcgggtc
     7801 cgacgcccgc tcgctcgaca gcctcgggga gggactgcga tgacccggac cgacatcccg
     7861 ccgagcgtca ccgtgacgaa accggccccg ccctcgtccg aggggcggac ccggcacacc
     7921 gtacggccct acgcgctgct gtggcggcgt gagatgaccc ggctgcggca caatccgctg
     7981 cgcctcgtca tgggcctggt caccccgctg ctgttcctcg tcgtgctggg cacggggctg
     8041 gaagcggcgt cgtccacgct cggcaaggcc cagctgaacg actaccgggc ctacctgttc
     8101 cctggcgcgc tcgtgatgtc cgtgcaggcc ccggccatcg ccgtgggcat ctcgctcgtc
     8161 tgggaccgcc ggctcggcat gctgcgccag atgctcgtct ccccgttccc gcggtcgagc
     8221 atcgtcctcg gcctcgcgct cggcggagcc accacgggcg ccgtctacgg cctcgccctg
     8281 ctcgcggtcg gaggcatcgc gggtgtccgg tacacgccga tgctgctggt cgtcctcgtc
     8341 gaactgctgc tggtctccct gctcttcacc tcgttcggcc ttctcgccgc ggtcaccatc
     8401 cggcaggtcg acaccttcca gatcgtggtg aacctcagcc tcatgccgct gatgttcttc
     8461 tccggcgcga tgttcccgcc caacgggctg ccgggctggc tcgacaccgt cgtcaagctg
     8521 aacccgctga cctacggcgt cgacgccgtg cgccggaccc tgcccggccc cgacgtcctc
     8581 acctcggagc agacccggct ggtgctcggc gactggaacc cgcccgtgta cgcggaactc
     8641 ggcgtgctgg ccgccctcac cgccgccgta ctgggcctcg ccacctaccg gttctcccgg
     8701 acacagtgaa agggggccgg ccgtgacgtt cgtgcagaca tgggcgggca ccgcggcccg
     8761 gatcgtcctc gcggcggtcc tgggctacgc gggctgggtg aaggtccagg acctcacggg
     8821 agccggccgt accgtcgccc tctaccagct cgtccccgag gaaagcgcac aactcgtcgg
     8881 cgccgccgtg gcgttcgtcg agctggcgct ggccctgctg cttctcgccg ggctcgccac
     8941 ccgcgccgtg gcggccgtca ccgccctgct gatggtcacg tacatcgcgg ccatcgcctc
     9001 ggtgtgggcg cgcgggatgt ccatcgactg cggctgcttc agcagcggcg gcaccctcac
     9061 cggcggtgcg gaacgcggct acgtcgtcga catcgcgcgc gacctcgcct tcctcggcgc
     9121 cgccgcgttc ctgatcaccc gcccgcggac ccgttacgcc ctggaccgct tggtcctgga
     9181 aacgaaggag cgatagccat gcagccgtcg acggacgccc aggtccggga aatggtccac
     9241 cggcgccggc gacgccgccg cactgtgctg gtgtcgctcg cggcggtggt cacggtgctc
     9301 ggcgccgcgc tcgtcggcgc cggcctggtc cgggccaccg acaccgagcc cggcgatgcg
     9361 cccgaggagg tgcccgccgg cgtggccgcc gaccgggccg gactcgtcac gtccgagggt
     9421 ccggtccgtg tcgacctcta cctcgactac ctctgccccg agtgccgcat caccgagaag
     9481 gccctcgcgc ccgaactgag ggagatgcag gaacgcggag aggtacgcgt cgtccaccac
     9541 ccggtcgcct tcctcgacga ccgcagcgcc cccgccggct actccacccg ggccgcgtcc
     9601 gccgccgcct gcgccgccga ccggaacaag ttcgagccct acacggcggc gctcttcgac
     9661 gagcagccgc cggagcaggg ccccggcctc gacaccgacc gcctggtcgc cctgggccgg
     9721 gacgtcggca tcaccggcgc ggccttcgag cggtgcgtcc gcgacggcac gtaccggccc
     9781 tgggtgacgt acgtgtccga cgtcgccgcc tcccgcgggg tggccctcac cccgaccgtg
     9841 aaggtggccg gcaagcgcgt ggagctctcc ggacccgacc gggcgaaggc gttcgtgcga
     9901 gcggtggagg agagccgttc atgagccgcc tcaccgtggc gctgaccggg gactgcatga
     9961 tgacgcgggg cgcgcccgtc acctccgacc ccgccgccgg ccgactgggc gaggtgctgc
    10021 acacggccga cttcgccttc accaacctgg aggtcgcgcc cgccaccgga cgaggccacc
    10081 ccgtacccga cgcggccagc ggaggcggcc tgatcgcgga ccccgccgtc ctcgacgacg
    10141 tgacggcgat gggcttctcc gtcctgagct gcgccaacaa ccatgccctc gacctcggca
    10201 cggagggagt cctcggcacg gtggacctgc tgcgcgccaa gggggtgccg ttcgcgggga
    10261 tcggcgccga cctcaccgcg gcccggcgcc cggtctacgt ggaccggccg gccggcagcc
    10321 tggcgctgat cgcgtgcacc gccaccttcc gggacgggca ggaggcggcc gccccgtccc
    10381 ccgacctgcc gggcaggccg ggactcagcc cgctgcggca cggcgtgacg ctgcgggtga
    10441 cggccgcgca gctggaggcg ttgcgggaga tcgacggcgc caccgggctg cggtcccggc
    10501 gggccgaggc gcgcaccctg ctcgggttcg accccgccat gcccggaccg ggccggctcg
    10561 cgctgttcgg cacccggttc gagaccgcgg acaccccggg cctcaccacc gcctgcgacc
    10621 cgcgggacgt ccacgacctc acccggtggg tggccgaggc gcgccgccgc gcggacctgg
    10681 tcctggtcag cgtccactcc cacgaacccg gcccgacgcc cgagacgccc gccgagttcc
    10741 tgcgggactt cgcccgtgcg gtgatcgacg ccggcgccga cgccgtggtc gggcacggac
    10801 cgcacatgct gcgcggtgtg gagctccacc ggggccggcc cgtcttccac agcctgggga
    10861 acatcgtcag ccagatcgaa ctggccgacc gcatccccgc cgaggactac gcgcgggccg
    10921 gtacgggcga ggccctgacc cccggcgggt acttcgagcg gatcaccgcc cacggccggc
    10981 ggatgttcgc cccgcaccgc cggtactggc agtcgctcgt gccggtgctc accttcgacg
    11041 acggggtctt cgccggcgcc cgcctgcacc cggtcgacct gggcttcggg cagcccgtgc
    11101 accggcgggg acggccccgg ctggccggtc cggtcgacgc caaggagatc ctcaccgact
    11161 tcgcgcggct cgcggagccc cacggcgtca cggtcaccgt cggggacgac gggacggggg
    11221 aactggtggc ggcggaaacc gacggcggcc accggcccgg ccgatagggt gccgggatac
    11281 aagctgacga ggcgtcggac cgccgggcac ggcggcgacc cgaggtacga gggatgcgca
    11341 atggccgagg cggtccgggt cctgatcgcg gacgaccagg cgctgctgcg cgggagcttc
    11401 cgggtcctcg tcgactcgac tcccggcatg gagacggtgg gggaggcggg ggacggcgcc
    11461 gaggccgtcg cgctcgcccg cgaactgacg ccggacgtgg tcctgatgga cctgcggatg
    11521 cccgtcatgg acggaatcgc cgccacccgc gagatctgct cggacgcgtc catggcgggc
    11581 gtgcgggtgc tggccctcac gatgttcgac atggacgagt acgtctaccc ggccctccag
    11641 gcgggcgcga gcggcttcat gctgaaggac acctcgccgg acgacctcgt cgcgggcatc
    11701 cgcgtcatcg ccaccggcga gggtgtgctg gccccctcgg tcacgcgtcg cgtcatcgcg
    11761 aacttctccc accgggacac ggccgcccgg ccggcccccc ggctcgtcgg gctcacccgg
    11821 cgcgaacagg aggtgctcgt gctgatcgcc aagggcctgt cgaacgcgga gatcgccgac
    11881 cggctggtga tcagcctgcc caccgtgaag acccacgtga gcagcctgct ctcgaagctg
    11941 aacgcccgcg accgcgccca gctggtcatc gtcgcctacg agagcggcat cgcggagcgc
    12001 gggatgccgc cggcctga
//
