BGC0002024 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002024 (Nocardia argentinensis)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,PKS-like	1	69,359	nargenicin A1/nargenicin A1	PKS:Type I	100%

BGC0002024 - Region 1 - PKS-like,T1PKS

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Location: 1 - 69,359 nt. (total: 69,359 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
PKS-like: (t2pks2 or ksIII)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 69,360
Label		BGC0002024

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002024	1.43	Polyketide	nargenicin	Nocardia argentinensis
BGC0001875	0.87	Polyketide	nargenicin A1	Nocardia arthritidis AUSMDU00012717 Unclassified.
BGC0002631	0.65	RiPP	myxarylin	Pyxidicoccus fallax
BGC0002347	0.54	Alkaloid	cyanogramide, cyanogramide B, cyanogramide C, cyanogramide D	Actinoalloteichus cyanogriseus
BGC0000131	0.54	Polyketide	pyrrolomycin	Streptomyces sp. UC 11065
BGC0001925	0.50	Alkaloid	altemicidin, SB-203207, SB-203208	Streptomyces sp.
BGC0001464	0.48	Other	tetrabromopyrrole	Pseudoalteromonas sp. PS5
BGC0001463	0.48	Other	2,3,4,5-tetrabromopyrrole	Pseudoalteromonas piscicida
BGC0002020	0.48	Saccharide, NRP	photolose	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000996	0.47	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002024	1.00	Polyketide	nargenicin	Nocardia argentinensis
BGC0001875	0.84	Polyketide	nargenicin A1	Nocardia arthritidis AUSMDU00012717 Unclassified.
BGC0000016	0.73	Other	amphotericin B	Streptomyces nodosus
BGC0000041	0.69	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0002631	0.66	RiPP	myxarylin	Pyxidicoccus fallax
BGC0001273	0.65	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001244	0.65	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0001163	0.64	Polyketide	1-heptadecene	Moorea producens 3L
BGC0000996	0.64	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78
BGC0002615	0.63	Terpene	4-oxomacrophorin A, macrophorin A, 5'-epimacrophorin B	Penicillium terrestre

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 69359:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-ohmal - Me-ccmal - ccmal) + (Me-ohmal - ohmal - ccmal) + (ccmal - ccmal)
Non-colinear ordering:: ngnA + ngnB + ngnC

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 68881:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-ohmal - Me-ccmal - ccmal) + (Me-ohmal - ohmal - ccmal) + (ccmal - ccmal)
Non-colinear ordering:: ngnA + ngnB + ngnC

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 44354 - 69359:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-ohmal - Me-ccmal - ccmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ngnC

PKS_AT (557..830): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 192.3
inactive: 89.8
Methoxymalonyl-CoA: 78.2
Methylmalonyl-CoA: 77.4
Isobutyryl-CoA: 50.9
Ethylmalonyl-CoA: 43.9
Propionyl-CoA: 41.5
Acetyl-CoA: 28.4
CHC-CoA: 19.7
trans-1,2-CPDA: 19.3
2-Methylbutyryl-CoA: 18.4
Benzoyl-CoA: 12.9
fatty_acid: 11.2
3-Methylbutyryl-CoA: 5.8

PKS_AT (2207..2476): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 184.3
inactive: 97.6
Methoxymalonyl-CoA: 73.2
Methylmalonyl-CoA: 73.0
Propionyl-CoA: 45.0
Acetyl-CoA: 38.8
Isobutyryl-CoA: 38.2
Ethylmalonyl-CoA: 37.8
2-Methylbutyryl-CoA: 27.5
fatty_acid: 18.1
CHC-CoA: 17.5
trans-1,2-CPDA: 16.0
Benzoyl-CoA: 14.4
3-Methylbutyryl-CoA: 0.0

ngnB

PKS_AT (564..853): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 208.1
Ethylmalonyl-CoA: 195.6
Methoxymalonyl-CoA: 165.5
2-Methylbutyryl-CoA: 147.0
trans-1,2-CPDA: 119.6
CHC-CoA: 115.7
Propionyl-CoA: 109.7
Malonyl-CoA: 97.6
Isobutyryl-CoA: 93.4
Benzoyl-CoA: 80.7
Acetyl-CoA: 40.8
3-Methylbutyryl-CoA: 35.3
fatty_acid: 27.7
inactive: 25.4

PKS_AT (2063..2337): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 183.4
inactive: 97.4
Methoxymalonyl-CoA: 64.1
Methylmalonyl-CoA: 60.0
Isobutyryl-CoA: 52.4
Propionyl-CoA: 37.0
Acetyl-CoA: 26.3
Ethylmalonyl-CoA: 25.7
2-Methylbutyryl-CoA: 17.8
3-Methylbutyryl-CoA: 12.4
fatty_acid: 11.9
trans-1,2-CPDA: 11.1
CHC-CoA: 10.6
Benzoyl-CoA: 9.1

PKS_AT (3563..3832): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 195.5
inactive: 95.7
Methylmalonyl-CoA: 79.6
Methoxymalonyl-CoA: 73.1
Ethylmalonyl-CoA: 55.4
Propionyl-CoA: 49.5
Isobutyryl-CoA: 42.2
2-Methylbutyryl-CoA: 38.1
Acetyl-CoA: 34.8
CHC-CoA: 24.8
trans-1,2-CPDA: 19.7
fatty_acid: 19.4
Benzoyl-CoA: 18.0
3-Methylbutyryl-CoA: 0.1

ngnA

PKS_AT (535..831): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 195.6
Ethylmalonyl-CoA: 186.0
Methoxymalonyl-CoA: 157.0
2-Methylbutyryl-CoA: 132.9
trans-1,2-CPDA: 111.2
Propionyl-CoA: 109.7
Malonyl-CoA: 101.5
CHC-CoA: 87.9
Isobutyryl-CoA: 82.6
Benzoyl-CoA: 70.6
Acetyl-CoA: 40.4
fatty_acid: 27.3
inactive: 26.9
3-Methylbutyryl-CoA: 17.1

PKS_AT (1525..1819): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 209.6
Ethylmalonyl-CoA: 192.2
Methoxymalonyl-CoA: 166.4
2-Methylbutyryl-CoA: 133.9
trans-1,2-CPDA: 118.4
Propionyl-CoA: 113.5
Malonyl-CoA: 103.8
CHC-CoA: 100.8
Isobutyryl-CoA: 79.7
Benzoyl-CoA: 73.1
Acetyl-CoA: 47.0
fatty_acid: 33.1
inactive: 20.9
3-Methylbutyryl-CoA: 14.7

PKS_AT (3035..3325): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 209.8
Ethylmalonyl-CoA: 188.8
Methoxymalonyl-CoA: 167.1
trans-1,2-CPDA: 120.3
2-Methylbutyryl-CoA: 108.5
Malonyl-CoA: 102.2
Propionyl-CoA: 94.3
CHC-CoA: 93.0
Isobutyryl-CoA: 88.9
Benzoyl-CoA: 78.3
Acetyl-CoA: 52.4
fatty_acid: 32.3
3-Methylbutyryl-CoA: 29.4
inactive: 25.0

PKS_AT (4747..5025): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Isobutyryl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 188.9
inactive: 96.7
Methylmalonyl-CoA: 70.4
Methoxymalonyl-CoA: 68.5
Isobutyryl-CoA: 52.7
Ethylmalonyl-CoA: 49.1
Propionyl-CoA: 37.3
Acetyl-CoA: 26.3
2-Methylbutyryl-CoA: 15.3
fatty_acid: 13.4
Benzoyl-CoA: 12.6
CHC-CoA: 10.3
trans-1,2-CPDA: 8.8
3-Methylbutyryl-CoA: -2.4

ngnN4

Search NORINE for peptide: strict or relaxed

AMP-binding (8..392): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLLYLALVCK
Nearest Stachelhaus code(s):: Pro DLLYLALVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)