BGC0002032 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002032 (Streptomyces sp. S816)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	108,545	High	pentamycin	PKS

BGC0002032 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 108,545 nt. (total: 108,545 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 108,546
Label		BGC0002032

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

DV517_01340: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

DV517_01350: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

DV517_01360: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

DV517_01420: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: acylase

DV517_01400: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase

DV517_01290: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: synthase

DV517_01370: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

DV517_01380: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

DV517_01390: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

DV517_01410: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002032.4	1.00	PKS	pentamycin	Streptomyces sp. S816
BGC0000059.5	0.85	PKS	filipin	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0002789.2	0.79	PKS	pentamycin, filipin	Streptomyces lavendulae strain YAKB-15
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000067.5	0.66	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002032.4	1.00	PKS	pentamycin	Streptomyces sp. S816
BGC0000059.5	0.85	PKS	filipin	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0002789.2	0.79	PKS	pentamycin, filipin	Streptomyces lavendulae strain YAKB-15
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000067.5	0.66	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15

Similar known clusters from MIBiG 4.0

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 108545:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - ohmal - ccmal - ccmal - ccmal) + (ccmal - pk) + (ccmal) + (ohmal - ohmal - ohmal - ohmal) + (ohmal - pk)
Non-colinear ordering:: DV517_01410 + DV517_01400 + DV517_01390 + DV517_01380 + DV517_01370

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

DV517_01370: mal - pk

PKS_AT (563..838): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 211.8
inactive: 110.3
Methoxymalonyl-CoA: 65.9
Methylmalonyl-CoA: 58.7
Propionyl-CoA: 29.2
Ethylmalonyl-CoA: 27.7
CHC-CoA: 22.3
Benzoyl-CoA: 20.6
Isobutyryl-CoA: 17.4
Acetyl-CoA: 17.0
2-Methylbutyryl-CoA: 14.6
trans-1,2-CPDA: 11.7
fatty_acid: 10.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (2068..2365): pk

ATSignature: Methoxymalonyl-CoA

Top 3 matches:: Methoxymalonyl-CoA: 79.2%; Ethylmalonyl-CoA: 75.0%; Methylmalonyl-CoA: 75.0%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 195.9
Methylmalonyl-CoA: 186.6
Methoxymalonyl-CoA: 175.4
2-Methylbutyryl-CoA: 137.1
trans-1,2-CPDA: 130.1
Propionyl-CoA: 108.1
CHC-CoA: 97.4
Isobutyryl-CoA: 92.5
Malonyl-CoA: 89.9
Benzoyl-CoA: 74.6
fatty_acid: 50.3
Acetyl-CoA: 48.3
3-Methylbutyryl-CoA: 33.8
inactive: 31.9

DV517_01380: mal - mal - mal - mal

PKS_AT (569..837): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 220.1
inactive: 115.9
Methoxymalonyl-CoA: 63.7
Methylmalonyl-CoA: 59.8
Propionyl-CoA: 34.5
Ethylmalonyl-CoA: 26.9
Isobutyryl-CoA: 21.9
Benzoyl-CoA: 21.3
2-Methylbutyryl-CoA: 19.4
Acetyl-CoA: 19.0
CHC-CoA: 16.9
trans-1,2-CPDA: 13.1
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2074..2350): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 217.0
inactive: 116.4
Methoxymalonyl-CoA: 59.9
Methylmalonyl-CoA: 59.7
Propionyl-CoA: 41.1
Ethylmalonyl-CoA: 27.6
Benzoyl-CoA: 22.8
2-Methylbutyryl-CoA: 22.0
fatty_acid: 20.7
Isobutyryl-CoA: 20.3
Acetyl-CoA: 19.4
CHC-CoA: 14.1
trans-1,2-CPDA: 11.4
3-Methylbutyryl-CoA: 0.0

PKS_AT (3576..3862): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 205.2
inactive: 105.0
Methoxymalonyl-CoA: 70.1
Methylmalonyl-CoA: 56.6
Propionyl-CoA: 30.6
Ethylmalonyl-CoA: 27.8
Isobutyryl-CoA: 24.8
2-Methylbutyryl-CoA: 16.8
Acetyl-CoA: 15.2
Benzoyl-CoA: 14.1
trans-1,2-CPDA: 9.0
CHC-CoA: 8.1
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (5110..5385): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 87.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 217.0
inactive: 113.4
Methoxymalonyl-CoA: 56.4
Methylmalonyl-CoA: 55.4
Propionyl-CoA: 33.2
Ethylmalonyl-CoA: 21.7
Isobutyryl-CoA: 20.2
Benzoyl-CoA: 18.6
fatty_acid: 18.4
2-Methylbutyryl-CoA: 18.1
CHC-CoA: 15.6
Acetyl-CoA: 14.9
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

DV517_01390: mal

PKS_AT (571..850): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 208.1
inactive: 105.2
Methoxymalonyl-CoA: 62.2
Methylmalonyl-CoA: 58.7
Propionyl-CoA: 44.5
Ethylmalonyl-CoA: 32.3
2-Methylbutyryl-CoA: 27.9
CHC-CoA: 24.2
Benzoyl-CoA: 23.1
Acetyl-CoA: 22.9
trans-1,2-CPDA: 18.4
Isobutyryl-CoA: 10.6
fatty_acid: 8.8
3-Methylbutyryl-CoA: 0.0

DV517_01400: mal - pk

PKS_AT (586..863): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 87.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 209.7
inactive: 120.2
Methoxymalonyl-CoA: 63.1
Methylmalonyl-CoA: 58.3
Propionyl-CoA: 33.5
Ethylmalonyl-CoA: 29.4
Acetyl-CoA: 23.6
Isobutyryl-CoA: 20.7
Benzoyl-CoA: 20.5
CHC-CoA: 19.1
2-Methylbutyryl-CoA: 18.5
trans-1,2-CPDA: 14.6
fatty_acid: 11.1
3-Methylbutyryl-CoA: 0.0

PKS_AT (2331..2626): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 87.5%; Malonyl-CoA: 83.3%; Methoxymalonyl-CoA: 83.3%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 196.8
Methylmalonyl-CoA: 192.1
Methoxymalonyl-CoA: 162.7
2-Methylbutyryl-CoA: 142.0
trans-1,2-CPDA: 125.9
Propionyl-CoA: 107.2
Malonyl-CoA: 88.9
CHC-CoA: 86.7
Isobutyryl-CoA: 79.7
Benzoyl-CoA: 64.1
Acetyl-CoA: 55.6
3-Methylbutyryl-CoA: 34.5
inactive: 23.9
fatty_acid: 20.7

DV517_01410: mal - mal - mal - mal - mal

PKS_AT (587..884): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 221.9
inactive: 123.9
Methoxymalonyl-CoA: 67.4
Methylmalonyl-CoA: 52.3
Isobutyryl-CoA: 36.0
Propionyl-CoA: 34.8
Acetyl-CoA: 18.8
trans-1,2-CPDA: 16.7
Benzoyl-CoA: 16.6
2-Methylbutyryl-CoA: 15.8
Ethylmalonyl-CoA: 12.5
CHC-CoA: 9.8
fatty_acid: 9.2
3-Methylbutyryl-CoA: 0.0

PKS_AT (1610..1885): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 204.2
inactive: 105.0
Methoxymalonyl-CoA: 54.5
Methylmalonyl-CoA: 48.9
Propionyl-CoA: 28.5
Ethylmalonyl-CoA: 25.9
Acetyl-CoA: 23.8
CHC-CoA: 23.7
Benzoyl-CoA: 22.7
2-Methylbutyryl-CoA: 20.1
Isobutyryl-CoA: 18.9
trans-1,2-CPDA: 14.3
fatty_acid: 8.9
3-Methylbutyryl-CoA: 0.0

PKS_AT (3124..3399): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 216.3
inactive: 118.0
Methoxymalonyl-CoA: 63.1
Methylmalonyl-CoA: 59.0
Propionyl-CoA: 39.4
Acetyl-CoA: 28.5
Benzoyl-CoA: 27.1
Isobutyryl-CoA: 25.2
Ethylmalonyl-CoA: 23.7
2-Methylbutyryl-CoA: 20.7
trans-1,2-CPDA: 17.1
CHC-CoA: 15.6
fatty_acid: 12.1
3-Methylbutyryl-CoA: 0.0

PKS_AT (4893..5169): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 212.5
inactive: 109.7
Methoxymalonyl-CoA: 64.5
Methylmalonyl-CoA: 57.8
Propionyl-CoA: 39.9
Ethylmalonyl-CoA: 30.6
Benzoyl-CoA: 27.4
Acetyl-CoA: 25.2
CHC-CoA: 23.9
Isobutyryl-CoA: 20.9
trans-1,2-CPDA: 19.3
2-Methylbutyryl-CoA: 19.0
fatty_acid: 13.1
3-Methylbutyryl-CoA: 0.0

PKS_AT (6582..6852): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 87.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.3
inactive: 108.0
Methoxymalonyl-CoA: 59.6
Methylmalonyl-CoA: 51.0
Propionyl-CoA: 35.2
Acetyl-CoA: 27.2
Ethylmalonyl-CoA: 22.8
Benzoyl-CoA: 22.3
CHC-CoA: 21.6
trans-1,2-CPDA: 17.4
Isobutyryl-CoA: 14.8
fatty_acid: 13.5
2-Methylbutyryl-CoA: 13.3
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor