LOCUS       BGC0002047             14165 bp    DNA     linear   BCT 16-FEB-2020
DEFINITION  Nocardia brasiliensis strain AUSMDU00024985 brasiliquinone gene
            locus, complete sequence.
ACCESSION   BGC0002047
VERSION     BGC0002047
KEYWORDS    .
SOURCE      Nocardia brasiliensis
  ORGANISM  Nocardia brasiliensis
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Nocardia.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 16:47:37
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14165
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..14165
                     /db_xref="taxon:37326"
                     /host="Homo sapiens"
                     /mol_type="genomic DNA"
                     /organism="Nocardia brasiliensis"
                     /strain="AUSMDU00024985"
     misc_feature    1..14165
                     /note="brasiliquinone gene locus"
     protocluster    1..14165
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[573:3041]"
                     /cutoff="20000"
                     /detection_rule="(t2ks and t2clf)"
                     /neighbourhood="35000"
                     /product="T2PKS"
                     /protocluster_number="1"
                     /t2pks_malonyl_elongations="8|9 (Score: 668.0; E-value:
                     9.4e-204)"
                     /t2pks_molecular_weights="acetyl-CoA_8 (Da): 365.355"
                     /t2pks_molecular_weights="acetyl-CoA_9 (Da): 407.391"
                     /t2pks_product_classes="angucycline"
                     /t2pks_starter_units="acetyl-CoA (Score: 0.0; E-value: 0)"
                     /tool="antismash"
     proto_core      574..3041
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(t2ks and t2clf)"
                     /neighbourhood="35000"
                     /product="T2PKS"
                     /protocluster_number="1"
                     /t2pks_malonyl_elongations="8|9 (Score: 668.0; E-value:
                     9.4e-204)"
                     /t2pks_molecular_weights="acetyl-CoA_8 (Da): 365.355"
                     /t2pks_molecular_weights="acetyl-CoA_9 (Da): 407.391"
                     /t2pks_product_classes="angucycline"
                     /t2pks_starter_units="acetyl-CoA (Score: 0.0; E-value: 0)"
     cand_cluster    1..14165
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(t2ks and t2clf)"
                     /kind="single"
                     /product="T2PKS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..14165
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002047"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..14165
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T2PKS"
                     /region_number="1"
                     /rules="(t2ks and t2clf)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            251..577
                     /gene="brqN"
     CDS             251..577
                     /codon_start=1
                     /gene="brqN"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1225:Polyketide synthesis cyclase (Score: 152.6;
                     E-value: 5.3e-47)"
                     /gene_functions="biosynthetic-additional (t2pks) CYC
                     C4-C17/C2-C19 (Score: 193.5; E-value: 8.2e-60)"
                     /gene_kind="biosynthetic-additional"
                     /note="BrqN"
                     /product="tetracenomycin F2 cyclase"
                     /protein_id="QHZ32172.1"
                     /transl_table=11
                     /translation="MHSTLIVARMDPGSHRQVADLFAEFDGTEMPHRMGTRRRQLFYYR
                     GLYFHLQDFDAADGGERIEEAKTDPRFVGISADLKPFIEAYDPQWRTPSDAMATRFYSW
                     AAES"
     gene            574..1845
                     /gene="brqA"
     CDS             574..1845
                     /EC_number="2.3.1.-"
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (5-397). E-value:
                     2.8e-55. Score: 179.8. Matches aSDomain:
                     nrpspksdomains_brqA_PKS_KS.1"
                     /codon_start=1
                     /gene="brqA"
                     /gene_functions="biosynthetic (rule-based-clusters) T2PKS:
                     t2ks"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 481.5; E-value:
                     3.8e-146)"
                     /gene_functions="biosynthetic-additional (t2pks) KS (Score:
                     760.4; E-value: 1e-231)"
                     /gene_kind="biosynthetic"
                     /note="BrqA"
                     /product="polyketide putative beta-ketoacyl synthase"
                     /protein_id="QHZ32173.1"
                     /sec_met_domain="t2ks (E-value: 1.9e-238, bitscore: 781.2,
                     seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNERRVVVTGMEVLAPGGVGRDAFWDLLSAGRTATRGITFFDPAP
                     FRSKIAAEVDFDPEANGLSPQQVRRMDRAAQFVAVAARDAVCDSGIDLNRYDPYRVGVT
                     IGTAVGAATSLDWEYRVVSDAGRLHLVDHEYAVPHLYNCLVPSSFAAEVAELVGARGAT
                     TVISTGCTSGLDSVGYAVDLIREGTADVMVAGATDAPISPITVASFDAIKATSPRNDEP
                     GTASRPFDGTRNGFVLGEGAAVFVLEELESAKRRGARIYAEIAGYATRSNAYHMTGLRP
                     DGVEMAEAIRVAMHEAEMNPDEIDYINAHGSGTKQNDRHETAAFKRSLGQRAYEVPVSS
                     IKSMVGHSLGAIGSIEIAASILAMTHNTVPPTANLHTPDPECDLDYVPLTAREHVTDAV
                     LTVGSGFGGFQSAMVLARAERSAA"
     aSDomain        589..1764
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_brqA_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="2.80E-55"
                     /label="brqA_PKS_KS.1"
                     /locus_tag="brqA"
                     /protein_end="397"
                     /protein_start="5"
                     /score="179.8"
                     /tool="antismash"
                     /translation="VVVTGMEVLAPGGVGRDAFWDLLSAGRTATRGITFFDPAPFRSKI
                     AAEVDFDPEANGLSPQQVRRMDRAAQFVAVAARDAVCDSGIDLNRYDPYRVGVTIGTAV
                     GAATSLDWEYRVVSDAGRLHLVDHEYAVPHLYNCLVPSSFAAEVAELVGARGATTVIST
                     GCTSGLDSVGYAVDLIREGTADVMVAGATDAPISPITVASFDAIKATSPRNDEPGTASR
                     PFDGTRNGFVLGEGAAVFVLEELESAKRRGARIYAEIAGYATRSNAYHMTGLRPDGVEM
                     AEAIRVAMHEAEMNPDEIDYINAHGSGTKQNDRHETAAFKRSLGQRAYEVPVSSIKSMV
                     GHSLGAIGSIEIAASILAMTHNTVPPTANLHTPDPECDLDYVPLTAREHVTD"
     gene            1854..3041
                     /gene="brqB"
     CDS             1854..3041
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (63-392). E-value:
                     7.6e-29. Score: 92.7. Matches aSDomain:
                     nrpspksdomains_brqB_PKS_KS.1"
                     /codon_start=1
                     /gene="brqB"
                     /gene_functions="biosynthetic (rule-based-clusters) T2PKS:
                     t2clf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1093:Beta-ketoacyl synthase (Score: 641.7; E-value:
                     6.5e-195)"
                     /gene_functions="biosynthetic-additional (t2pks) CLF 8|9
                     (Score: 668.0; E-value: 9.4e-204)"
                     /gene_kind="biosynthetic"
                     /note="BrqB"
                     /product="polyketide putative beta-ketoacyl synthase 2"
                     /protein_id="QHZ32174.1"
                     /sec_met_domain="t2clf (E-value: 3.2e-200, bitscore: 654.7,
                     seeds: 26, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVTGFGVAAPNGLGAEQYWAATVAGKSGIARVSRFDPSGYPSQLA
                     GEVPGFEAAEHLPGRLLPQTDRMTQLALAATDWAFADAGLTPDRLPEFDMGVITASSAG
                     GFEFGQRELRNLWQRGGQYVSAYQSFAWFYAVNSGQIAIRNGMKGPASVLVSDQAGGLD
                     AVAAARRAIRKGTSTIVTGAIDSSLCPWGWIGQQASGRLSTSDDPADAYLPFDARANGH
                     VPGEGGAILLVESDAHARARGARAYGEIAGYAATFDPAGPGLRKAIEAALADAGRTPAD
                     IDVVFADAAGLPELDRDEARAISEVFGAGAVPVTAPKTMTGRLYSGAGPLDLVAALLTM
                     RHGLIPPTVNIEADPEARMDLVLGQARPAEVRTALVLARGYGGFNSALVLSNENS"
     aSDomain        2043..3029
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_brqB_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="7.60E-29"
                     /label="brqB_PKS_KS.1"
                     /locus_tag="brqB"
                     /protein_end="392"
                     /protein_start="63"
                     /score="92.7"
                     /tool="antismash"
                     /translation="QTDRMTQLALAATDWAFADAGLTPDRLPEFDMGVITASSAGGFEF
                     GQRELRNLWQRGGQYVSAYQSFAWFYAVNSGQIAIRNGMKGPASVLVSDQAGGLDAVAA
                     ARRAIRKGTSTIVTGAIDSSLCPWGWIGQQASGRLSTSDDPADAYLPFDARANGHVPGE
                     GGAILLVESDAHARARGARAYGEIAGYAATFDPAGPGLRKAIEAALADAGRTPADIDVV
                     FADAAGLPELDRDEARAISEVFGAGAVPVTAPKTMTGRLYSGAGPLDLVAALLTMRHGL
                     IPPTVNIEADPEARMDLVLGQARPAEVRTALVLARGYGGFNSALVLSN"
     CDS_motif       2496..2534
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_brqB_0001"
                     /evalue="8.90E-04"
                     /label="PKSI-KS_m4"
                     /locus_tag="brqB"
                     /protein_end="227"
                     /protein_start="214"
                     /score="12.4"
                     /tool="antismash"
                     /translation="FDARANGHVPGEG"
     gene            3054..3311
                     /gene="brqC"
     CDS             3054..3311
                     /NRPS_PKS="Domain: ACP (20-81). E-value: 3.6e-13. Score:
                     41.5. Matches aSDomain: nrpspksdomains_brqC_ACP.1"
                     /codon_start=1
                     /gene="brqC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1129:acyl carrier protein (Score: 96.7; E-value:
                     1e-29)"
                     /gene_functions="biosynthetic-additional (t2pks) ACP
                     (Score: 104.8; E-value: 4.3e-33)"
                     /gene_kind="biosynthetic-additional"
                     /note="BrqC"
                     /product="acyl carrier protein"
                     /protein_id="QHZ32175.1"
                     /sec_met_domain="PP-binding (E-value: 1.1e-12, bitscore:
                     38.2, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADGFTLTDLGRIMLEAAGDDGVDFTEPVLDVLFDDLGYDSLALL
                     EASGRIEREFDISLDEDTLLAATTPRALIGAVNARLANAA"
     aSDomain        3114..3296
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_brqC_ACP.1"
                     /evalue="3.60E-13"
                     /label="brqC_ACP.1"
                     /locus_tag="brqC"
                     /protein_end="81"
                     /protein_start="20"
                     /score="41.5"
                     /tool="antismash"
                     /translation="DGVDFTEPVLDVLFDDLGYDSLALLEASGRIEREFDISLDEDTLL
                     AATTPRALIGAVNARL"
     gene            3324..4109
                     /gene="brqD"
     CDS             3324..4109
                     /EC_number="1.3.1.-"
                     /NRPS_PKS="Domain: PKS_KR (7-162). E-value: 9e-12. Score:
                     37.1. Matches aSDomain: nrpspksdomains_brqD_PKS_KR.1"
                     /codon_start=1
                     /gene="brqD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     254.4; E-value: 1.8e-77)"
                     /gene_functions="biosynthetic-additional (t2pks) KR C9
                     (Score: 466.5; E-value: 5.2e-143)"
                     /gene_kind="biosynthetic-additional"
                     /note="BrqD"
                     /product="putative ketoacyl reductase"
                     /protein_id="QHZ32176.1"
                     /sec_met_domain="adh_short (E-value: 2e-37, bitscore:
                     118.8, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKIEAPVALVTGATSGIGLAVARLLGGSGHRVFIGARTEENVIA
                     TVKQMRSEGLTVDGAAVDVRSDAEVQQFVQAAVDAFGSIDVLVNNAGRSGGGVTADLTA
                     ELWNDVIETNLTSVFRLTREVLNTGGMRTKNRGRIVNIASTAGKQGVVLGAPYSASKHG
                     VVGFTKALGNELAPTGITVNAVCPGYVETPMAARVRQGYADAYGKSEAEILEKFQAKIP
                     LGRYSTPEEVAGLVGYLTTDTAASITAQALNVCGGLGNF"
     aSDomain        3345..3809
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_brqD_PKS_KR.1"
                     /evalue="9.00E-12"
                     /label="brqD_PKS_KR.1"
                     /locus_tag="brqD"
                     /protein_end="162"
                     /protein_start="7"
                     /score="37.1"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VALVTGATSGIGLAVARLLGGSGHRVFIGARTEENVIATVKQMRS
                     EGLTVDGAAVDVRSDAEVQQFVQAAVDAFGSIDVLVNNAGRSGGGVTADLTAELWNDVI
                     ETNLTSVFRLTREVLNTGGMRTKNRGRIVNIASTAGKQGVVLGAPYSASKH"
     CDS_motif       3351..3419
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_brqD_0001"
                     /evalue="2.50E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="brqD"
                     /protein_end="32"
                     /protein_start="9"
                     /score="13.7"
                     /tool="antismash"
                     /translation="LVTGATSGIGLAVARLLGGSGHR"
     gene            4131..5078
                     /gene="brqE"
     CDS             4131..5078
                     /NRPS_PKS="Domain: Polyketide_cyc2 (6-150). E-value:
                     4.6e-14. Score: 44.9. Matches aSDomain:
                     nrpspksdomains_brqE_Polyketide_cyc2.1"
                     /NRPS_PKS="Domain: Polyketide_cyc2 (159-307). E-value:
                     1e-08. Score: 27.5. Matches aSDomain:
                     nrpspksdomains_brqE_Polyketide_cyc2.2"
                     /codon_start=1
                     /gene="brqE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1128:cyclase/dehydrase (Score: 428.2; E-value:
                     3e-130)"
                     /gene_functions="biosynthetic-additional (t2pks) CYC C7-C12
                     (Score: 461.0; E-value: 3.3e-141)"
                     /gene_kind="biosynthetic-additional"
                     /note="BrqE"
                     /product="putative polyketide cyclase"
                     /protein_id="QHZ32177.1"
                     /transl_table=11
                     /translation="MTTSSTMREVEHEIEIAAPADTVYALIADVGSWPRIFGPTIYVEH
                     LEQTEGSERIRVWATANGTAKNWTSRRTLDPTARKITFQQEVSAPPVAFMSGAWLIEAR
                     SANSALVRLQHTYRAVDDDPDGLAWIDEAVDRNSRSELEGLRVELEFAYAATEATFSFE
                     DSVQIAGSATRAYEFIDQAQLWTERLPHVAAARLEEPAPGLQTLEMDTKTRDGSVHTTK
                     SYRVTCPHHSIVYKQVTLPALMSLHTGRWTFAENADGVLATSQHTVTINTERIASILGA
                     DKTLEDAKDFVRNALSTNSMTTLRHAKSFAERRD"
     aSDomain        4149..4580
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_brqE_Polyketide_cyc2.1"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="4.60E-14"
                     /label="brqE_Polyketide_cyc2.1"
                     /locus_tag="brqE"
                     /protein_end="150"
                     /protein_start="6"
                     /score="44.9"
                     /tool="antismash"
                     /translation="MREVEHEIEIAAPADTVYALIADVGSWPRIFGPTIYVEHLEQTEG
                     SERIRVWATANGTAKNWTSRRTLDPTARKITFQQEVSAPPVAFMSGAWLIEARSANSAL
                     VRLQHTYRAVDDDPDGLAWIDEAVDRNSRSELEGLRVELE"
     aSModule        4149..5051
                     /domains="nrpspksdomains_brqE_Polyketide_cyc2.1"
                     /domains="nrpspksdomains_brqE_Polyketide_cyc2.2"
                     /incomplete
                     /locus_tags="brqE"
                     /tool="antismash"
                     /type="unknown"
     aSDomain        4608..5051
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_brqE_Polyketide_cyc2.2"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="1.00E-08"
                     /label="brqE_Polyketide_cyc2.2"
                     /locus_tag="brqE"
                     /protein_end="307"
                     /protein_start="159"
                     /score="27.5"
                     /tool="antismash"
                     /translation="FSFEDSVQIAGSATRAYEFIDQAQLWTERLPHVAAARLEEPAPGL
                     QTLEMDTKTRDGSVHTTKSYRVTCPHHSIVYKQVTLPALMSLHTGRWTFAENADGVLAT
                     SQHTVTINTERIASILGADKTLEDAKDFVRNALSTNSMTTLRHA"
     gene            5082..6536
                     /gene="brqF"
     CDS             5082..6536
                     /EC_number="1.14.13.38"
                     /codon_start=1
                     /gene="brqF"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050:monooxygenase FAD-binding (Score: 494.4; E-value:
                     6.8e-150)"
                     /gene_functions="biosynthetic-additional (t2pks) OXY
                     (Score: 519.1; E-value: 2.8e-158)"
                     /gene_kind="biosynthetic-additional"
                     /note="BrqF"
                     /product="anhydrotetracycline monooxygenase"
                     /protein_id="QHZ32178.1"
                     /transl_table=11
                     /translation="MIEQTQVVVVGAGPVGLMLAGELRLGGVEVVVLEARATPSTESRA
                     STLHARTMEIFDARGLVDALGTVPNEPRGHFGGIALDLTMPSRFPGQWKVPQTRTVELL
                     HDWAVALGAEVRRGWSVSAVRDDGAFVEVHARTTAGTRVLRADFVVGCDGADSTVRACT
                     GMAFEGRAATRELLRADVAGLAVEDRRFQRYEHGFATAVTNPAGITRVMVHRFGTGGAE
                     RVGEPSFDAVVAAWQQVTGEAIGHGKPLWVNAFDDASKQVRAYRAGRVLFAGDAAHQQM
                     PSGGQALNLGLQDAVNLGWKLARAIDARAEHLLDTYHAERSAVGARILSNIRVQTELLL
                     GGPEVESLRTMLRDLIAAEPVRAHLAGMVSGLDVRYPAEPGEHPWLGLRLPEVRLREPA
                     GTSGAALRTGAGLVLGFGPAQVEALAKVVAPWSKRVVVAEVAAGAEIGTEAVLVRPDGY
                     VSWAGSADADLDGRLRHWFGPPGAEH"
     gene            6565..8076
                     /gene="brqG"
     CDS             6565..8076
                     /EC_number="1.14.13.38"
                     /codon_start=1
                     /gene="brqG"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050:monooxygenase FAD-binding (Score: 543.9; E-value:
                     6.5e-165)"
                     /gene_functions="biosynthetic-additional (t2pks) OXY
                     (Score: 571.6; E-value: 3.2e-174)"
                     /gene_kind="biosynthetic-additional"
                     /note="BrqG"
                     /product="anhydrotetracycline monooxygenase"
                     /protein_id="QHZ32179.1"
                     /transl_table=11
                     /translation="MDTDVIVVGAGPTGLLLAGELRLAGADVVVLEQLPEPTGQSRGLG
                     FTARAMETFDQRGLLAAVGGLEQSPMGHFGGLLFDYTVLEDCHFGARGVPQSQTEAALE
                     RWAGGLGADIRRGWTVTGLDDDGTGVAVTARLCGGEKVLRARYLVGCDGGHSIVRKLAG
                     FEFPGTAGTLGMYLADVVGCDIAPRPFGERLPGGMIMAMPLAPGVDRVVVCEHETPVVQ
                     RGTEIGFAELAAAWERITGQSIAHGRAAWASSFSDATRQAAEYRRGRVFLAGDAAHIHL
                     PAGGQGLSTGVQDAANLGWKLGAVVRGSAGPELLDTYHDERHPVGARLLDNTRVQGMLY
                     ITGAEADPLRELFTELIAYDDTKRYLAGAVSGLDIRYESNPSAHPLQGRRIPPRPVRTS
                     NGPTTTTELLHPARGVLLDLVGDSALQDAAAPWRDRVTAVGAELSERRGLEDVTAVLIR
                     PDGYVAWAAAEQRDRTALTAALRRWFGPADGVARAGAATVGEALR"
     misc_feature    6625..6627
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            8073..8984
                     /gene="brqH"
     CDS             8073..8984
                     /EC_number="4.2.99.20"
                     /codon_start=1
                     /gene="brqH"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /note="BrqH"
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="QHZ32180.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 1.7e-16, bitscore:
                     51.3, seeds: 455, tool: rule-based-clusters)"
                     /sec_met_domain="PF00561 (E-value: 3.7e-12, bitscore: 36.3,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTELARSSGVRPVLLDGGGITLSALLAQPYEAPRATIVALHGA
                     GMSAGYFDGQATAEVSLLSLGARLGYTVLAVDRPGYGHSSAQLREGQSQAAQARTVRHA
                     LAGFAAENDLGAGILLLGHSFGGMLALRLAAVAFGTARLLGVDISGCGHRYARQLFQLG
                     DARRRSLRGWGPPRLYPLATFRASAAIVAPTPVLELAEAPRWPMVFPRVAAKVAVPVRC
                     TFAEHEGLWRQDPNTVRDMAARFTGAPCVAVERQAGAGHNISLSWAARAYHLRVLGFLE
                     DCLHADDRQASDRPIPRVGTEV"
     gene            9016..9885
                     /gene="brqI"
     CDS             9016..9885
                     /EC_number="2.7.7.24"
                     /codon_start=1
                     /gene="brqI"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 270.5; E-value:
                     2.8e-82)"
                     /gene_kind="biosynthetic-additional"
                     /note="BrqI"
                     /product="glucose-1-phosphate thymidylyltransferase 1"
                     /protein_id="QHZ32181.1"
                     /sec_met_domain="NTP_transf_3 (E-value: 1.9e-13, bitscore:
                     40.9, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKGIILAGGSGTRLQPVTRAVSKQLLPVYDKPMIYYPLSVLMLAG
                     ITDIEIIATPDDLPRFQRLLGDGSALGLCLTYAEQDKPRGLADAFLVSERHIGNEPVAL
                     VLGDNIFHGPGFVEVLQQSSHEVDGCVLFGYPVLDPQRYGVAEVDTAGNLLDLVEKPAR
                     PQSNLAVTGLYFYHNDVVAIAKELQPSARGELEITDVNRTFLAQDRARLVRLGRGFVWL
                     DAGTHEALLEAGQYVEIMERRQGVRIACIEEIALRMGFIDQESCHRLGRKLAKSPYGRY
                     VMDIAAAV"
     gene            9942..11063
                     /gene="brqJ"
     CDS             9942..11063
                     /EC_number="2.6.1.106"
                     /codon_start=1
                     /gene="brqJ"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1056:DegT/DnrJ/EryC1/StrS aminotransferase (Score:
                     400.6; E-value: 1.2e-121)"
                     /gene_kind="biosynthetic-additional"
                     /note="BrqJ"
                     /product="dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose
                     transaminase"
                     /protein_id="QHZ32182.1"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 1.6e-121,
                     bitscore: 395.8, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTRVWDYLPEYEKERTDILDAVQTVFGSGQLILGESVRGFEREF
                     AAYHGLAHCTGVDNGTNALKLGLEALGVGPGDEVITVANTAAPTVVAIVGAGATPVFVD
                     VRADDFLMDTDQVAAAITPRTKAIVPVHLYGQCVDMAPLRRLAAEHGLQILEDCAQAHG
                     ARHGDRLAGTMSNAAAFSFYPTKVLGAYGDGGAVITADEVVQRNMRQLRYYGMDKVYYV
                     MRTPGHNSRLDEVQAEILRRKLGRLDEYIAGRNTVARRYEQGLGDLADSHGLLLPATNP
                     GNTHVYYVYVVRHPKRDAIIEAMREHDILLNISYPWPVHTMTGFEHLGWRAGSLPVTER
                     LAEEIFSLPMYPALGEAEQDRVIEVLRTVVAGL"
     gene            11060..12442
                     /gene="brqK"
     CDS             11060..12442
                     /codon_start=1
                     /gene="brqK"
                     /note="BrqK"
                     /product="NDP-hexose 2,3-dehydratase"
                     /protein_id="QHZ32183.1"
                     /transl_table=11
                     /translation="MSVRSVRTRSDEDLSERLALSAAATEAGAWLRTADFPGWLAQRAA
                     ANRFEVRRIPFLEMDAWSFAPDTGNMRHRTGKFFSVEGLSVQVDEGAVRAWQQPIILQP
                     EVGILGILAKEFDGVLHFLMQAKMEPGNRNLLQLSPTVQATRSNYTGVHRGSTVRYLSY
                     FADLGAGRVLADVLQSEHGAWFYRKSNRNMLVETTDEVAVHPDFCWLTLGQIGALLRAD
                     NVVNMDSRTVIATFPMHGTSAVALRSDAELQSWFTVERARHDVEISLMPLAEVAGWHRG
                     ESHLGRADDRFFRLIAVEVAAGSREVTSWTQPLFEPHGQGIAAFVFRRIDGLPHLLVQA
                     KVEAGFLDTVELAPTVQCVPANWDQHPPFLDVIRAASPEQIRYDVVHSEEGGRFLNAES
                     RYMFVEADLPLAVPRNYCWATPAQLNWLAGHGHYLNVQARTLLSCLNAGAVSLNQDFAH
                     YG"
     gene            12435..12866
                     /gene="brqL"
     CDS             12435..12866
                     /codon_start=1
                     /gene="brqL"
                     /note="BrqL"
                     /product="hypothetical protein"
                     /protein_id="QHZ32184.1"
                     /transl_table=11
                     /translation="MVDVSERLRAVLDRAELVELFDRYVHHLDFDRDNDAWLGDVFTED
                     AELVFPHGTFRGMAELREFQRMAYAKYPRSHHLGGNHRVLRDGDTATVTAHLTAVHIGA
                     AVGPTGQMSFGGFGTAAAVRTAAGWRMRRFTFTSAWTRP"
     gene            complement(12872..13603)
                     /gene="brqM"
     CDS             complement(12872..13603)
                     /codon_start=1
                     /gene="brqM"
                     /gene_functions="regulatory (smcogs) SMCOG1008:response
                     regulator (Score: 173; E-value: 1.1e-52)"
                     /gene_kind="regulatory"
                     /note="BrqM"
                     /product="sensory transduction protein regX3"
                     /protein_id="QHZ32185.1"
                     /transl_table=11
                     /translation="MELQNFDADRSASHHILLVESNEAERRSLTERLVRHGYSVTAVDT
                     AGSALECYHNADLVLLDVDLSDLDGIEVCRAIRDTSAVPIIIVTARDSVLDRVLGLRSG
                     ADDYITKPYGARELIARIQAVLRRFVPEECLAPTPVPATSIRHGPLSVDADARRVELAG
                     DLVKTTRKEFDLLYLLASNPGKVIERSTITEEIWDGTLSSRTVDTHINSLRKKLGASEW
                     IVTVRGIGFRFIGHRYDAQHA"
     misc_feature    complement(13301..13303)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 agttcgctga cactccctgt gctctaccgg atttcggcta tacggagtgg cgtcgaaagt
       61 ttgacaatgt cctgaccgat tcgaatcttg attattgtcg gcgccttcaa gatcatggaa
      121 gccggggtcg aaatcgctac ccgccgcgtg gagtaacgaa tcctcgccag tgcagtccgc
      181 agtccgctcg ccgcgatcgg cgattttctg ggattgcgag cgtcgaatct agtagaagga
      241 gacaactccg atgcacagca cattgattgt ggcgcggatg gatcccggat cacatcggca
      301 ggtcgcggac ctgttcgccg aattcgatgg cacggagatg ccgcaccgca tgggtacccg
      361 tcgcaggcag ctgttctatt accgcggact ctatttccac ctgcaggatt tcgacgccgc
      421 ggacggcggc gagcggatcg aggaggccaa gaccgatccg cgcttcgtcg gcatcagcgc
      481 cgacctgaag ccgttcatcg aggcctacga cccgcagtgg cgcaccccct ccgacgccat
      541 ggccacccgg ttctacagct gggcagccga atcgtgaacg agcgaagagt cgtcgtcacc
      601 ggcatggagg tgctcgcacc cggcggcgtc ggtcgcgacg cgttctggga tctgctgagc
      661 gcgggccgca cggcgacccg gggcatcacc ttcttcgatc cggcgccgtt ccggtccaag
      721 atcgccgcgg aggtcgactt cgatcccgag gccaacggcc tcagcccgca gcaggtccgg
      781 cggatggacc gggccgcgca gttcgtcgcc gtcgcggcac gtgatgcggt gtgcgacagt
      841 gggatcgacc tcaaccgcta tgacccgtac cgggtcggtg tcacgatcgg caccgcggtc
      901 ggcgcggcca ccagcctgga ctgggaatac cgcgtggtca gcgatgccgg ccggctgcat
      961 ctggtcgacc acgaatacgc cgtgccgcac ctgtacaact gcctggtgcc gagctcgttc
     1021 gccgcggagg tggccgagct ggtcggggcg cgcggagcta ccacggtgat ctcgaccggc
     1081 tgcacctccg gcctcgactc ggtcggctac gcggtggatc tgatccgcga gggcaccgcc
     1141 gatgtcatgg tggcgggcgc caccgacgcg ccgatctccc cgatcaccgt cgccagcttc
     1201 gacgcgatca aggcgacctc gccgcgcaac gacgagcctg gcaccgcttc gcgtccgttc
     1261 gacggcaccc gcaacggctt cgtgctcggc gagggcgccg cggtgttcgt gctcgaggag
     1321 ctcgagtcgg ccaagcggcg cggcgcgcgc atctatgccg agatcgcggg ctacgcgacc
     1381 cgcagcaacg cctaccacat gaccggactg cggcccgacg gcgtcgagat ggccgaggcc
     1441 atccgggtcg ccatgcacga ggccgagatg aacccggacg agatcgacta catcaacgcg
     1501 cacggctcgg gcaccaagca gaacgaccgg cacgagaccg cggccttcaa gcgcagcctc
     1561 gggcagcggg cctacgaggt gccggtgagc tcgatcaaat cgatggtcgg gcattcgctc
     1621 ggcgcgatcg gctcgatcga gatcgccgcc tcgattctgg cgatgacgca caacaccgtg
     1681 ccgccgacgg cgaacctgca caccccggac ccggagtgcg atctggacta tgtgccgctg
     1741 acagcgcgcg agcacgtgac cgacgcggtg ctgaccgtgg gtagcgggtt cggcggtttc
     1801 cagagcgcga tggtgctggc ccgcgccgaa cggagcgcgg catgacgcgg gcggtggtca
     1861 ccggattcgg cgtcgcggca ccgaacggcc ttggtgcgga gcagtattgg gccgcgaccg
     1921 tggcgggtaa gtcgggcatc gccagggtgt cccggttcga cccgagcgga tatccgtccc
     1981 agctcgccgg tgaggtcccc ggcttcgagg ccgccgagca tctgccgggc aggctgctgc
     2041 cgcagacgga tcggatgacc cagctggcct tggccgcgac ggactgggcg ttcgccgatg
     2101 ccggactgac gccggaccgg ttgccggaat tcgacatggg cgtgatcacc gccagctcgg
     2161 ccggtgggtt cgagttcggt cagcgcgagc tgcgcaacct ctggcagcgc ggcggccagt
     2221 acgtcagcgc gtaccagtcc ttcgcctggt tctacgccgt gaacagcggc cagatcgcga
     2281 ttcgcaacgg catgaaggga ccggccagcg tgctggtcag cgatcaggcg ggcggactgg
     2341 acgccgtcgc cgcggcccgg cgcgcgattc gcaagggcac cagcaccatc gtgacgggcg
     2401 cgatcgactc gtcgctgtgc ccgtggggct ggatcgggca gcaggcgagc gggcgattga
     2461 gcaccagcga cgacccggcc gacgcctacc tgcccttcga tgcccgcgcg aacgggcacg
     2521 tgcccggcga gggcggcgcg atcctgctcg tcgagtccga tgcgcacgcg cgggcccgcg
     2581 gtgcccgcgc ctacggcgag atcgccggct acgcggcgac tttcgatccg gcggggccgg
     2641 gcttgcgcaa ggcgatcgag gcggcgctgg ccgatgccgg gcggacaccg gcggacatcg
     2701 acgtggtgtt cgccgacgcg gccggcttgc cggaactcga tcgcgatgag gcgcgggcga
     2761 tttcggaagt attcggggcc ggcgccgtgc cggtcaccgc gccgaagacg atgaccggcc
     2821 ggttgtactc cggtgccggg ccgctcgatc tcgtcgccgc gctgctgacc atgcgccacg
     2881 gcctgatccc accgacggtc aacatcgagg ccgatcccga ggcgcggatg gacctggtgc
     2941 tcggccaggc ccgcccggcc gaggtgcgca ccgcgctggt cctggcgcgg ggctacggcg
     3001 gattcaactc cgcgctcgtg ctgagcaacg aaaactcatg aaggagaaca acaatggctg
     3061 acggattcac actcaccgac ctgggccgga tcatgctcga ggccgcgggc gacgacgggg
     3121 tcgacttcac cgagcccgtg ctcgacgtgc tcttcgacga cctcggttac gactcgctcg
     3181 cgctgctcga ggccagcggc cggatcgagc gcgagttcga catctcgctg gacgaggaca
     3241 ccctgctcgc cgccaccacg ccgcgggcac tgatcggtgc cgtcaacgcg cggctcgcga
     3301 acgcggcctg aggaggagca cagatgtcga agatcgaagc gcccgtcgcc ctggtgaccg
     3361 gggcgaccag cggtatcgga ctggccgtcg cccgcctgct cggcggctcg ggacaccggg
     3421 tgttcatcgg cgcgcgcacc gaggagaacg tgatcgccac ggtgaaacag atgcgctcgg
     3481 aaggacttac ggtggacggc gccgcggtgg atgtgcgctc cgacgccgag gtgcagcagt
     3541 tcgtccaggc cgctgtcgac gcgttcgggt cgatcgatgt gctggtgaac aacgcgggcc
     3601 gcagcggcgg cggcgtcacc gccgatctca ccgccgaact ctggaacgac gtcatcgaga
     3661 ccaatctgac cagcgtattc cggctgaccc gcgaggtgct caacaccggc ggcatgcgca
     3721 cgaagaaccg cggccggatc gtcaacatcg cctccaccgc gggcaagcag ggcgtggtgc
     3781 tcggcgcgcc gtactcggcg tcgaagcacg gcgtcgtcgg cttcaccaag gcgctcggta
     3841 atgagctggc ccccaccggc atcacggtca acgccgtgtg ccccggttac gtggagacgc
     3901 ctatggcggc gcgggtccgg cagggatacg ccgacgccta cggcaagagc gaggccgaga
     3961 tcctggagaa gttccaggcc aagatcccac tcgggcgcta ctccaccccg gaggaggtgg
     4021 ccggattggt cggctacctg accaccgaca cggccgcatc catcacggcg caagcgttga
     4081 acgtctgcgg cggacttggc aatttctgag ctcgaggagt aggtaattcg atgaccacca
     4141 gcagcaccat gcgcgaggtc gagcacgaga tcgagatcgc cgcacccgcc gacacggtgt
     4201 acgcgctgat cgccgatgtc ggcagctggc cgcgcatctt cggtccgacg atctacgtcg
     4261 agcacctgga gcagaccgag ggcagcgagc ggatccgggt ctgggccacc gccaacggca
     4321 ccgcgaagaa ctggacctca cgccgcacgc tcgatccgac ggcccggaaa atcaccttcc
     4381 agcaagaggt ttcggcgccg ccggtggcct tcatgagcgg cgcgtggctg atcgaggcgc
     4441 gttcggcgaa ctccgccctg gtccggctgc aacacaccta ccgcgccgtg gacgatgatc
     4501 cggacggtct cgcctggatc gacgaggcgg tcgaccgcaa ttcgcgcagt gaactggagg
     4561 ggctgcgggt cgagctcgaa ttcgcttatg ccgcgacgga ggccaccttc tccttcgagg
     4621 actcggtgca gatcgccggg tcggccacac gggcatacga attcatcgat caggcacagc
     4681 tgtggaccga gcggctgccg cacgtcgccg cggcgcggct ggaagaaccc gcacccggct
     4741 tgcagacact cgagatggac accaagacgc gcgacggctc ggtgcacacc acgaagtcct
     4801 atcgggtcac ctgcccgcac cactcgatcg tctacaagca ggtgaccctg ccggcgctga
     4861 tgtcgttgca cacggggcgg tggaccttcg ccgagaacgc cgacggcgtg ctggcgacct
     4921 cgcagcacac cgtcacgatc aacaccgagc ggatcgcctc gatcctcggc gcggacaaga
     4981 cgctagagga tgccaaggat ttcgtgcgga acgcgttgag caccaacagc atgaccactt
     5041 tgcggcacgc gaagtccttc gccgagcgac gcgactgaca gatgatcgaa cagacgcagg
     5101 tagtcgtcgt cggcgcgggc ccggtcgggc tcatgctggc gggtgagttg cgcctcggcg
     5161 gcgtcgaggt ggtggtgctg gaggcgcgtg cgacgccgtc gacggagtcg cgggcctcga
     5221 ccctgcacgc gcggacgatg gagatcttcg acgcccgcgg cctggtcgac gcgctcggca
     5281 ccgtgccgaa cgaaccgcgc ggccacttcg ggggcatcgc gctcgatctc accatgccga
     5341 gccggtttcc cggacagtgg aaggtaccgc agaccagaac cgttgagttg ttgcatgatt
     5401 gggccgtcgc gctgggggcc gaggtccggc gcggctggtc ggtgtcggcg gtgcgcgacg
     5461 acggtgcctt cgtcgaggtt cacgcgcgga ccacggccgg tacccgagtg ctgcgtgcgg
     5521 acttcgtcgt cggctgcgac ggcgcggaca gcacggtgcg cgcgtgtacc ggcatggcgt
     5581 tcgagggcag ggcggcgacc cgcgaactgc tgcgcgcgga cgtggcggga ctggccgtcg
     5641 aggaccggcg attccagcgc tacgagcacg gattcgccac ggcggtcacg aatcccgccg
     5701 gcatcacccg ggtgatggtg catcgcttcg gtaccggtgg cgccgagcgg gtgggcgagc
     5761 cgtccttcga tgcggtcgtc gcggcgtggc agcaggtcac cggtgaggcg atcgggcatg
     5821 gaaagccgct gtgggtgaac gccttcgacg atgcgagcaa acaggtgcgg gcctaccgcg
     5881 ccgggcgcgt gctgttcgcg ggcgatgccg cgcatcagca gatgcccagc ggcggtcaag
     5941 cgctgaacct aggcctgcag gatgccgtga acctgggttg gaagctggcc cgcgccatcg
     6001 acgcgcgggc cgagcacctg ctcgatacct atcacgccga acgcagtgcg gtcggcgcgc
     6061 gaatcttgtc gaacatccgg gtgcaaaccg aattgttgct cggcgggccg gaagtcgagt
     6121 cgctgcggac catgctgcgg gacctgatcg cggcggagcc ggtgcgtgcg cacctagccg
     6181 ggatggtcag cggattggac gtgcgctatc ccgcggaacc gggggagcac ccctggctcg
     6241 gcctccggct gcccgaagtg cggctgcgcg agcccgccgg gacgagcggc gcggcactgc
     6301 gcaccggggc cggcctcgtg ctcggcttcg gccccgcaca ggtcgaggcg ctggcgaagg
     6361 tggtggcgcc ctggtccaag cgggtcgtcg tggccgaggt cgccgccggt gccgagatcg
     6421 gcaccgaggc ggtgctggta cggccggacg gctatgtgag ctgggcgggt tccgcggacg
     6481 cggacctcga cggccggctg cgccactggt tcgggccgcc cggtgccgag cactgaccgc
     6541 gccgtgcaga caaggagagc gaacgtggac accgatgtga tcgtggtcgg cgcgggtccg
     6601 accggcctgc tgctagccgg cgaattacgg ctggccggtg ccgatgtcgt ggtgctggaa
     6661 caactgccgg aaccgacagg ccagtcccgc ggcctcggct tcaccgcccg cgcgatggag
     6721 accttcgacc agcgcggcct gctcgcggcc gtcggcgggc tggagcagag cccgatgggc
     6781 cacttcggcg gcctgctgtt cgactacacg gtgctggagg actgtcattt cggggcgcgc
     6841 ggggtgccgc aatcgcagac cgaggcggcg ctggagcggt gggcgggcgg gctcggcgcg
     6901 gacatccggc ggggctggac agtgaccggt ctggatgacg acgggaccgg cgtcgcggta
     6961 accgcgcgac tctgcggcgg tgaaaaggtc ttgcgcgcaa ggtatctcgt cggctgcgac
     7021 ggcgggcaca gcatcgtgcg caaactcgcc ggcttcgagt ttcccggcac ggcaggcact
     7081 ctcggcatgt atctggccga cgtggtcggt tgcgacatcg ccccgcgccc gttcggcgag
     7141 cgcctgcccg gcgggatgat catggccatg ccgctggcgc ccggcgtgga tcgggtcgtg
     7201 gtctgcgagc acgagacacc ggtggtgcag cgcggcaccg agatcggctt cgccgagctc
     7261 gccgcggcgt gggaacgcat cacggggcag tcgatcgccc acggccgcgc ggcgtgggcc
     7321 agttcgttct ccgacgccac ccggcaggcg gcggaatacc gcaggggccg cgtgttcttg
     7381 gcgggcgacg cggcacatat ccacctcccg gcgggcggtc aggggttgag caccggtgtt
     7441 caggacgcgg ccaatctcgg gtggaaactc ggcgccgtgg tgcgcggatc ggccgggccg
     7501 gagctgctcg acacctacca cgacgaacgt catccggtcg gcgcccggct gctggacaac
     7561 acccgggtgc aagggatgct ctatatcacc ggggccgagg cggatccgct gcgcgagctg
     7621 ttcacggagc tgatcgccta cgacgacacc aagcgctacc tcgcgggcgc ggtgagcggg
     7681 ctcgacatcc gatacgagtc gaatccttcc gcacatccgt tgcagggcag gcggattccg
     7741 ccacggccgg tgcgcacgtc gaatggcccg accacgacca ccgaactgct gcatccggcg
     7801 cgcggtgtgc tgctggacct tgtcggcgac agcgcgttgc aggacgccgc ggcaccgtgg
     7861 cgggaccggg tcaccgccgt cggcgccgag ctgtccgagc ggcgcggtct cgaggacgtg
     7921 accgccgtgc tgatccgccc ggacggctac gtggcgtggg cggcggccga acagcgggat
     7981 cgcacggcgt tgacggcggc cctgcgccgc tggttcggtc cggcggacgg ggtggcccgt
     8041 gccggggccg ccacggtcgg cgaggcgctg cggtgacgac cacggaacta gcccgttcca
     8101 gcggcgtgcg gccggtgctg ctggatggcg gcgggatcac gttgtccgcg ctactcgctc
     8161 agccctacga agcgccgcgc gcgacgatcg tggcgctgca cggggccggg atgagtgcgg
     8221 gctacttcga cgggcaggcg acggccgagg tatcgctgct cagtctcggt gcgcgcctcg
     8281 gctatacggt gctggcggtg gaccggccgg gctacggcca ttcgagtgcg caactgcgcg
     8341 aaggacaatc gcaggcggcg caggcgcgca ccgtgcggca tgcgctcgcc ggattcgccg
     8401 ccgagaacga cctcggtgcc gggatactgc tgctgggaca ttctttcggc ggcatgctcg
     8461 cgctgcggct ggccgccgtg gcattcggta cggcccgcct gctcggcgtg gacatctccg
     8521 ggtgcgggca tcgatacgcg cggcagctgt ttcagctcgg cgacgcgcgc aggcggtcgc
     8581 tgcgtgggtg gggaccgccg cggttgtacc cgcttgccac ctttcgcgcc agcgcggcca
     8641 tcgtcgcgcc gacgccggtc ctcgagctgg ccgaggcgcc gcgttggccc atggtgttcc
     8701 cgcgcgtcgc ggccaaggtg gcggtgccgg tccggtgcac cttcgccgaa cacgagggac
     8761 tgtggcgcca ggacccgaac acggtgcggg acatggccgc gcgcttcacc ggtgcgccgt
     8821 gcgtggcggt ggagcggcag gcgggcgccg ggcacaacat cagcctgagt tgggccgcac
     8881 gcgcctatca cctgcgggtg ctcggattcc tcgaggactg tctgcacgcc gacgaccggc
     8941 aggcatccga tcgtccgata ccgcgcgtcg ggaccgaagt atgaggccgc acaagcaatc
     9001 ccggaggagt tggcggtgaa ggggataatt ctcgcaggcg gcagcggcac ccgcttgcaa
     9061 ccggtcaccc gggcggtgtc gaagcagctg ctgccggtct acgacaagcc gatgatctac
     9121 tacccgctct cggtgctgat gctggccggt atcaccgata tcgagatcat cgcaacgccg
     9181 gacgatctgc cgcggttcca gcggttgctc ggtgacggat cggcgctcgg gttgtgcctg
     9241 acctacgccg agcaggacaa gccgcgcggg ctggccgacg cgttcctggt ctccgagcgg
     9301 cacatcggca acgagccggt ggccctggtg ctgggagaca acatctttca cgggccgggc
     9361 ttcgtcgagg tcctgcagca gagcagccat gaggtcgacg gatgcgtcct gttcggctat
     9421 ccggtgctcg acccgcaacg ctacggggtc gctgaggtgg ataccgcggg caacctgctc
     9481 gatctggtgg agaagcccgc caggccgcag tcgaacctcg ccgtcaccgg gctgtacttc
     9541 tatcacaacg acgtcgtcgc gatcgccaag gagctacagc cctccgcgcg tggcgaattg
     9601 gagatcaccg atgtgaaccg gaccttcctg gcacaggacc gggcgcggct ggtgcggctc
     9661 ggccgcggct tcgtctggct cgacgcgggc acccacgagg ccctgctgga ggcggggcag
     9721 tacgtggaga tcatggagcg ccgccaaggc gtacggatcg cctgcatcga ggagatcgcc
     9781 ttgcgcatgg gcttcatcga ccaggagtcg tgccaccggc tcggccgcaa actcgcgaaa
     9841 tcaccctacg gccgatacgt catggacatc gcggcagccg tgtgagctgc cccggaccga
     9901 cgacgttcgt tccgatcatt cgaatcacca agggagcatt catgaccacc cgtgtatggg
     9961 actacctgcc cgagtacgag aaggaacgga ccgacatcct cgacgcggtg cagaccgtgt
    10021 tcgggtcggg gcagcttatt ctcggcgaga gcgtgcgcgg cttcgagcgc gaattcgcgg
    10081 cctaccacgg gctcgcgcac tgcaccggcg tcgacaacgg caccaacgca ctgaaactcg
    10141 gcctcgaggc gctcggcgtc ggtcccggtg acgaggtgat caccgtcgcg aacaccgcgg
    10201 cgcccaccgt ggtggcgatc gtcggcgcgg gcgcgacacc ggttttcgtc gatgtgcgcg
    10261 ccgatgactt cctcatggac accgatcagg tggcagcggc gatcacaccg cgtaccaaag
    10321 cgattgtgcc cgtgcatctt tacggtcagt gcgtcgacat ggcgccgctg cggcggctgg
    10381 ccgccgaaca cggtctgcag atcctggagg actgtgctca ggcacacggc gcacgccacg
    10441 gggaccgcct ggcgggcacc atgtcgaacg ccgcggcatt ctccttctat ccgaccaagg
    10501 tgctcggcgc ctacggggac ggcggcgcgg tgatcaccgc cgacgaggtc gtgcagcgaa
    10561 atatgcggca gctgcgctac tacggcatgg acaaggtcta ctacgtgatg cgcacgccgg
    10621 gccacaattc ccggctcgac gaggtgcagg cggagatcct gcgccgcaag ctcggtcggc
    10681 tcgacgaata catcgcgggc cgcaataccg tggcgcggcg ctacgagcaa ggcctcggcg
    10741 acctggccga ttcgcacggg ttgctgctgc ccgcgacgaa tcccggcaat acccatgtct
    10801 attacgtgta cgtggtgcgg cacccgaagc gcgacgcgat catcgaggcg atgcgcgagc
    10861 acgacattct gctcaacatc agctacccgt ggccggtgca cacgatgacc gggttcgagc
    10921 atctcggttg gcgtgcaggg tcgttgccgg tcaccgagcg gctggcggag gagatcttct
    10981 cgctgccgat gtatccggcg ttgggcgagg ccgagcagga ccgtgtcatc gaggtgttgc
    11041 gcaccgtcgt ggcggggctg tgagcgtgcg ctccgttcgg acccgctccg acgaggatct
    11101 gtcggagcgg ctcgcgctct cggccgcggc caccgaagcc ggtgcgtggc tgcgcaccgc
    11161 ggacttcccg gggtggctcg cgcagcgggc ggcggctaac cgattcgagg tgcggcgcat
    11221 cccgtttctc gagatggatg cctggtcctt cgcgccggac accgggaaca tgcgccaccg
    11281 caccggaaag ttcttcagcg tcgaggggct ctcggtgcag gtggacgagg gggcggtgcg
    11341 cgcgtggcag cagccgatca tcctgcaacc cgaggtcggg atactcggca tcctcgccaa
    11401 ggaattcgac ggtgtgctgc actttctcat gcaggccaag atggagccgg gcaaccgcaa
    11461 tctgctccag ctctcgccga cggtgcaggc cacccgcagc aactacaccg gcgtgcatcg
    11521 ggggagcacc gtccgatacc tgtcctactt cgccgatctc ggcgcgggcc gggtgctcgc
    11581 cgacgtactg cagtccgaac acggtgcctg gttctatcgc aagtcgaacc ggaacatgct
    11641 ggtggagacc accgatgagg tcgcggtgca tccggacttc tgctggctga ccctcggcca
    11701 gatcggtgcg ctgctgcggg cggacaacgt ggtgaacatg gactcgcgca ccgtcatcgc
    11761 gacctttccg atgcacggaa ccagcgctgt cgcactgcgt tcggatgccg agctgcaatc
    11821 gtggttcacc gtcgagcgcg ccaggcacga cgtggagatc tcgctgatgc cgctggccga
    11881 ggtcgcggga tggcaccgcg gtgagtccca cctcggtcgt gccgacgaca ggttcttccg
    11941 gctcatcgcg gtcgaggtgg cggcgggcag cagggaggtc acctcgtgga cccagccgct
    12001 gttcgagccg cacggccagg gcatcgccgc cttcgtcttc cgtcggatcg acggtctgcc
    12061 acatcttttg gtgcaggcga aagtggaggc cgggttcctc gacaccgtcg agctggcgcc
    12121 gaccgtgcag tgcgtgccag cgaactggga ccagcacccg ccgttcctgg acgtgatccg
    12181 tgccgccagt ccggagcaga tccgttatga cgtagtgcat tcggaggagg gcgggcggtt
    12241 tctcaacgcg gagagccgct acatgttcgt cgaggccgat ctgccgctgg cggtgccgcg
    12301 caactactgc tgggccaccc ccgcgcaatt gaactggctg gccggccacg gtcactatct
    12361 caacgtgcag gccaggacgt tgctgtcgtg cctcaatgcc ggggccgttt cgctgaatca
    12421 ggatttcgca cactatggtt gacgtttcag agcgcctgcg ggcagtcctc gaccgcgccg
    12481 agctggtcga gctcttcgat cgctacgtgc accatctcga cttcgaccgg gacaacgacg
    12541 cctggctcgg cgacgtgttc acagaagacg ccgagctcgt gttcccgcac ggcacctttc
    12601 gcggcatggc cgagctgcgc gagttccagc gcatggccta cgcgaagtac ccgcgctcgc
    12661 accatctcgg cggcaatcat cgggtgctgc gcgacggcga caccgcgacc gtgacagcac
    12721 atctcaccgc ggtgcacatc ggcgcggcgg tgggcccgac cgggcagatg tccttcggcg
    12781 ggttcggcac ggccgccgcg gtgcgtaccg cggcgggctg gcggatgcgc cgcttcacct
    12841 tcacctcggc gtggacgcgc ccataacggt gctacgcgtg ctgtgcgtcg tatcggtgac
    12901 cgatgaagcg gaagccgata ccgcgcacgg tgacgatcca ctcgctcgca ccgagcttct
    12961 tgcggaggct gttgatgtgg gtgtccaccg tccggctcga caacgtgccg tcccagatct
    13021 cctcggtgat cgtgctgcgc tcgatcacct tgcccggatt ggaggcgagc aggtagagca
    13081 ggtcgaattc ctttctcgtc gtcttgacga gatctccggc caattcgacc cttctggcgt
    13141 cggcgtcgac cgagagtgga ccgtgccgga tcgaggtcgc gggcaccggg gtgggcgcca
    13201 gacattcctc gggcacgaag cggcgcaata cggcttggat ccgcgcgatc aattcgcggg
    13261 cgccgtatgg cttcgtgatg tagtcgtcgg cgccggaacg taatccgagc acccgatcca
    13321 gcaccgagtc ccgcgcggtc acaatgatga tcgggaccgc gctggtgtcg cggatggcgc
    13381 ggcagacctc gatgccgtcc aggtcggaca gatcgacgtc gagcaacacc agatcggcat
    13441 tgtggtaaca ctccagcgca ctgcccgcgg tgtcgaccgc ggtcaccgag tagccgtgtc
    13501 ggacgaggcg ctcggtcagc gaccgacgtt ctgcctcatt gctttccacc aataggatgt
    13561 ggtggctcgc cgaccggtcg gcgtcgaaat tttgtagctc catattcccc cgatagatca
    13621 cagctcgtac tgcggattgc ggtgggtgag cgcattactc cgacgggtgc gccgtcgggg
    13681 ctgggctgcc cggtctttct ggaattactt cggcgcgcgg acttttcctt gccgggtggc
    13741 acacgtggtg ccccgtgacc ggaaagtgat acgcgacagc cgggctgctc gacgctgggc
    13801 agctgggctg aggtagactg aagacggtcg aattatgttg tcgcagtgct gatttggatg
    13861 tgccctccat tccactcgat gctgcccgga gatcctatca gatgggatga tcccaggcct
    13921 ctttattgtc atgtgacaca acatatgtcg attgcatcgc ccgattcccg cgggccgcgg
    13981 atagtagatg acaagctaat gatccaatct tcgaacaata cgaactaaac ttgtcgattc
    14041 gcgtatcaca tagtggtgcc cacactttcg tggtcacgat ctgatgaagc cgctgtggta
    14101 ctgtcgggtt tgccgtcgga gggcgacctc cggtccgacg gacgcccagc tcagggcgtg
    14161 tttta
//
