BGC0002050: necroxime A biosynthetic gene cluster from Burkholderia sp. B8(2020)
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Location: 1 - 103,471 nt. (total: 103,471 nt).
This entry is originally from NCBI GenBank MN734804.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
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General
Compounds
Genes
Biosynthesis
History
KnownClusterBlast
NRPS/PKS domains
General information about the BGC
MIBiG accession BGC0002050
Short description necroxime A biosynthetic gene cluster from Burkholderia sp. B8(2020)
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Trans-AT type I)
Loci
MN734804.1
20001 - 86009
via Knock-out studies
Compounds
  • necroxime A
  • necroxime B
  • necroxime C
  • necroxime D
Species Burkholderia sp. B8(2020) [taxonomy]
References
Chemical products information
necroxime A Evidence: NMR [1]
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C33H44N4O11
Chemical database entries
NPAtlas
necroxime B Evidence: NMR [1]
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Chemical database entries
NPAtlas
necroxime C Evidence: NMR [1]
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Chemical database entries
NPAtlas
necroxime D Evidence: NMR [1]
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Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • QIE07358.1
 19778 - 20026 (-) hypothetical protein
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    • QIE07359.1
    • necA
    		 20001 - 24038 (-) dimodular nonribosomal peptide synthase NecA
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      • QIE07360.1
      		 24767 - 25099 (+) hypothetical protein
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        • QIE07361.1
        • necB
        		 25498 - 27393 (+) polyketide synthase NecB
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          • QIE07362.1
          • necC
          		 27383 - 32962 (+) polyketide synthase NecC
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            • QIE07363.1
            • necD
            		 32959 - 36894 (+) polyketide synthase NecD
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              • QIE07364.1
              • necE
              		 36973 - 45459 (+) polyketide synthase NecE
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                • QIE07365.1
                • necF
                		 45462 - 64364 (+) polyketide synthase NecF
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                  • QIE07366.1
                  • necG
                  		 64348 - 71727 (+) polyketide synthase NecG
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                    • QIE07367.1
                    • necH
                    		 71724 - 83471 (+) polyketide synthase NecH
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                      • QIE07368.1
                      • necI
                      		 83617 - 85017 (+) epi-isozizaene 5-monooxygenase/(E)-beta-farnesene synthase
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                        • QIE07369.1
                        • necJ
                        		 85239 - 86009 (+) 4'-phosphopantetheinyl transferase superfamily protein
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                          Biosynthesis information

                          Biosynthetic modules

                          Name
                          6
                          Type
                          nrps-type1
                          Genes
                          necE
                          Substrates
                          glycine (evidence: Structure-based inference [2])
                          Integrated Monomers
                          Domains
                          condensation (LCL), adenylation
                          Name
                          2
                          Type
                          nrps-type1
                          Genes
                          necC
                          Substrates
                          glycine (evidence: Structure-based inference [2])
                          Integrated Monomers
                          Domains
                          adenylation
                          Name
                          1
                          Type
                          nrps-type1
                          Genes
                          necA
                          Substrates
                          lysine (evidence: Structure-based inference [2])
                          Integrated Monomers
                          Domains
                          condensation (Starter), adenylation
                          Name
                          3
                          Type
                          pks-trans-at
                          Genes
                          necC
                          Substrates
                          Integrated Monomers
                          Domains
                          ketosynthase, oxidase
                          Name
                          4
                          Type
                          pks-trans-at
                          Genes
                          necD
                          Substrates
                          Integrated Monomers
                          Domains
                          ketosynthase, dehydratase, methyltransferase, carrier (ACP)
                          Name
                          5
                          Type
                          pks-trans-at
                          Genes
                          necE
                          Substrates
                          Integrated Monomers
                          Domains
                          ketosynthase, dehydratase, ketoreductase, carrier (ACP), carrier (ACP)
                          Name
                          7
                          Type
                          pks-trans-at
                          Genes
                          necF
                          Substrates
                          Integrated Monomers
                          Domains
                          ketosynthase, dehydratase, ketoreductase, carrier (ACP), carrier (ACP)
                          Name
                          8
                          Type
                          pks-trans-at
                          Genes
                          necF
                          Substrates
                          Integrated Monomers
                          Domains
                          ketosynthase, ketoreductase, carrier (ACP)
                          Name
                          9
                          Type
                          pks-trans-at
                          Genes
                          necF
                          Substrates
                          Integrated Monomers
                          Domains
                          ketosynthase, dehydratase, ketoreductase, carrier (ACP)
                          Name
                          10
                          Type
                          pks-trans-at
                          Genes
                          necF
                          Substrates
                          Integrated Monomers
                          Domains
                          ketosynthase, ketoreductase, carrier (ACP)
                          Name
                          11
                          Type
                          pks-trans-at
                          Genes
                          necF necG
                          Substrates
                          Integrated Monomers
                          Domains
                          ketosynthase, dehydratase, dehydratase, carrier (ACP)
                          Name
                          12
                          Type
                          pks-trans-at
                          Genes
                          necG
                          Substrates
                          Integrated Monomers
                          Domains
                          ketosynthase, ketoreductase, carrier (ACP)
                          Name
                          13
                          Type
                          pks-trans-at
                          Genes
                          necG necH
                          Substrates
                          Integrated Monomers
                          Domains
                          ketosynthase, dehydratase, dehydratase, carrier (ACP)
                          Name
                          14
                          Type
                          pks-trans-at
                          Genes
                          necH
                          Substrates
                          Integrated Monomers
                          Domains
                          ketosynthase, carrier (ACP)
                          Name
                          15
                          Type
                          pks-trans-at
                          Genes
                          necH
                          Substrates
                          Integrated Monomers
                          Domains
                          ketosynthase, ketoreductase, carrier (ACP)
                          Name
                          16
                          Type
                          pks-trans-at
                          Genes
                          necH
                          Substrates
                          Integrated Monomers
                          Domains
                          ketosynthase, dehydratase, thioesterase, carrier (ACP)
                          Annotation changelog

                          Entry version: 3

                          Date
                          Changes
                          Submitters
                          Reviewers
                          2024-05-23
                          MIBiG v4 annotathon
                          • (ID: YO3TP6VWB5CBNPZKNYNQSKUW)
                          • (ID: I3D2K5SU7JJDS2LEEQM3W3SO)
                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                          Entry version: 2

                          Date
                          Changes
                          Submitters
                          Reviewers
                          2022-10-07
                          Update chemical activity to schema version 2.11
                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                          Entry version: 1

                          Date
                          Changes
                          Submitters
                          Reviewers
                          2022-09-15
                          Submitted
                          • (ID: Z7AIIJAM3U3HR6IYDPQOINM4)
                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                          2022-09-15
                          Corrected NRP module activity
                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                          2022-09-15
                          Removed erroneous gene name
                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                          2022-09-15
                          Removed gene annotations with no information
                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                          2022-09-15
                          Removed ketoreductase stereochemistry annotation from modules without ketoreductases
                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                          2022-09-15
                          Merged cross-CDS PKS modules
                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                          2022-09-15
                          Sorted modules by module number
                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                          2022-09-15
                          Added version to NCBI accession
                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                          2022-09-15
                          Added extra compounds from annotathon data
                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                          2022-09-15
                          Updated NRP substrate specificities
                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
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