BGC0002050: necroxime A biosynthetic gene cluster from Burkholderia sp. B8(2020)
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Location: 20,001 - 86,009 nt. (total: 66,009 nt).
This entry is originally from NCBI GenBank MN734804.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
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General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0002050
Short description necroxime A biosynthetic gene cluster from Burkholderia sp. B8(2020)
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP
  • Polyketide (Other)
Loci NCBI GenBank: MN734804.1
Compounds
  • necroxime A
  • necroxime B
  • necroxime C
  • necroxime D
Species Burkholderia sp. B8(2020) [taxonomy]
References
Chemical products information
necroxime A
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C33H44N4O11
Chemical database entries
NPAtlas
necroxime B
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Chemical database entries
NPAtlas
necroxime C
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Chemical database entries
NPAtlas
necroxime D
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Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • QIE07359.1
  • necA
 20001 - 24038 (-) dimodular nonribosomal peptide synthase NecA
  • Tailoring (Unknown)
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  • QIE07360.1
 24767 - 25099 (+) hypothetical protein
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  • QIE07361.1
  • necB
 25498 - 27393 (+) polyketide synthase NecB
  • Scaffold biosynthesis
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  • QIE07362.1
  • necC
 27383 - 32962 (+) polyketide synthase NecC
  • Scaffold biosynthesis
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  • QIE07363.1
  • necD
 32959 - 36894 (+) polyketide synthase NecD
  • Scaffold biosynthesis
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  • QIE07364.1
  • necE
 36973 - 45459 (+) polyketide synthase NecE
  • Scaffold biosynthesis
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  • QIE07365.1
  • necF
 45462 - 64364 (+) polyketide synthase NecF
  • Scaffold biosynthesis
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  • QIE07366.1
  • necG
 64348 - 71727 (+) polyketide synthase NecG
  • Scaffold biosynthesis
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  • QIE07367.1
  • necH
 71724 - 83471 (+) polyketide synthase NecH
  • Scaffold biosynthesis
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  • QIE07368.1
  • necI
 83617 - 85017 (+) cytochrome P450
  • Tailoring (Dioxygenation)
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  • QIE07369.1
  • necJ
 85239 - 86009 (+) 4'-phosphopantetheinyl transferase superfamily protein
  • Tailoring (Monooxygenation)
Sequence-based prediction
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Polyketide-specific information
Subclass Other
Starter unit Malonyl-CoA
Cyclic? yes
Release type
  • Macrolactonization
Polyketide-synthases
Genes Properties Modules
necG
+
necB
+
necF
+
necE
+
necD
+
necH
+
necC 		Synthase subclass: Trans-AT type I
Trans-acyltansferases: necB
Module 3
Genes: necC
Core domains: Ketosynthase, OXY
Module 4
Genes: necD
Core domains: Thiolation (ACP/PCP), Ketosynthase, Dehydratase, MT
Module 5
Genes: necE
Core domains: Thiolation (ACP/PCP), Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 7
Genes: necF
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP), Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 8
Genes: necF
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 9
Genes: necF
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 10
Genes: necF
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 11
Genes: necF, necG
Core domains: Ketosynthase, Dehydratase, Thiolation (ACP/PCP), Dehydratase
Module 12
Genes: necG
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 13
Genes: necG, necH
Core domains: Ketosynthase, Dehydratase, Thiolation (ACP/PCP), Dehydratase
Module 14
Genes: necH
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module 15
Genes: necH
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 16
Genes: necH
Core domains: Ketosynthase, Dehydratase, Thiolation (ACP/PCP), Thioesterase
NRP-specific information
Subclass N/A
Cyclic? yes
Release type
  • Macrolactonization
NRP-synthases
Gene Modules
necE
Module 6
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
necC
Module 2
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
necA
Module 1
Specificity: lysine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Starter
Annotation changelog
MIBiG version Submitter Notes
3.0
  • Hidden contributor (ID: Z7AIIJAM3U3HR6IYDPQOINM4, no GDPR consent given).
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Submitted
  • Corrected NRP module activity
  • Removed erroneous gene name
  • Removed gene annotations with no information
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Merged cross-CDS PKS modules
  • Sorted modules by module number
  • Added version to NCBI accession
  • Added extra compounds from annotathon data
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
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