LOCUS       BGC0002052              4080 bp    DNA     linear   BCT 06-OCT-2020
DEFINITION  Streptomyces sp. strain ID38640 ectoine biosynthetic gene cluster,
            complete sequence.
ACCESSION   BGC0002052
VERSION     BGC0002052
KEYWORDS    .
SOURCE      Streptomyces sp
  ORGANISM  Streptomyces sp.
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-08 06:19:41
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4080
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /db_xref="taxon:1931"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces sp."
                     /strain="ID38640"
     subregion       1..4080
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="Other"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..4080
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(189..1088)
                     /gene="ectD"
                     /locus_tag="NAI38-5748"
     CDS             complement(189..1088)
                     /codon_start=1
                     /gene="ectD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="NAI38-5748"
                     /product="ectoine dioxygenase"
                     /protein_id="QOE83927.1"
                     /sec_met_domain="PhyH (E-value: 7.5e-41, bitscore: 128.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGTAEVITPRKDPVVWSQPGTAGPFEPSELS
                     DFERDGFFAIGELLTAEEVAVYRAELDRLVLDPTMRADPRSIVEPKSQDVRTVFEVHKI
                     SEVFAKLVSDPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     NMRTVSVSIALTENYDTNGGLMIMPGSHEYFVGCEGATPKDNYKRSLQMQDAGIPSDEV
                     LTKMADKHSIRLFTGKAGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSVENAAVEPFA
                     APVRRPEYIGARDFTPVR"
     misc_feature    189..3620
                     /note="ectoine biosynthetic gene cluster"
     gene            complement(1184..1600)
                     /gene="ectC"
                     /locus_tag="NAI38-5749"
     CDS             complement(1184..1600)
                     /codon_start=1
                     /gene="ectC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /locus_tag="NAI38-5749"
                     /note="L-ectoine synthase"
                     /product="EctC"
                     /protein_id="QOE83928.1"
                     /sec_met_domain="ectoine_synt (E-value: 1e-56, bitscore:
                     178.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKAKSGTWESKRIVLAKERVGFSLHETTLY
                     AGTETSMWYANHIEAVLCVEGEAELTNDETGEKYTITPGTMYLLDGHEKHTMRIKEDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEPESEPETV"
     gene            complement(1647..2915)
                     /gene="ectB"
                     /locus_tag="NAI38-5750"
     CDS             complement(1647..2915)
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 5.3e-76.
                     Score: 247.6. Matches aSDomain:
                     nrpspksdomains_NAI38-5750_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013:aminotransferase class-III (Score: 380.1;
                     E-value: 2.2e-115)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="NAI38-5750"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="QOE83929.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.2e-82, bitscore:
                     264.9, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRSWPTVFDRAQGSRMTDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGVTHGLDMSTTAKRAFLESFQNIILRPRD
                     LPYKVMFPGPTGANSVEAALKLARKVKGRESIVSFTNAFHGMSLGALAVTGNSMKRAGA
                     GIPLVHGTPMPFDNYLDGTYPDFLWFERLLEDQGSGLNTPAAVIVETVQGEGGINVARP
                     EWLRALADLCERWDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPLA
                     LTLFKPELDIWEPGEHNGTFRGNNPAFVTAAATLDTYWADGQMEKQTLARGEIVEAHLK
                     AITEEHPDAIAEYRGRGLVWGMELNDKPTADKIAKRAFELGLLIETSGPESEVVKLLPA
                     LTTTPEELDEGLRILARAVRDCV"
     aSDomain        complement(1821..2831)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NAI38-5750_Aminotran_3.1"
                     /evalue="5.30E-76"
                     /label="NAI38-5750_Aminotran_3.1"
                     /locus_tag="NAI38-5750"
                     /protein_end="365"
                     /protein_start="28"
                     /score="247.6"
                     /tool="antismash"
                     /translation="FDRAQGSRMTDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGVTHGLDMSTTAKRAFLESFQNIILRPRDLPYKVMFPGPTGANSVEAALKLARKVKG
                     RESIVSFTNAFHGMSLGALAVTGNSMKRAGAGIPLVHGTPMPFDNYLDGTYPDFLWFER
                     LLEDQGSGLNTPAAVIVETVQGEGGINVARPEWLRALADLCERWDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPLALTLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAATLDTYWADGQMEKQTLARGEIVEAHLKAITEEHPDAIAEYRGRGLVWGMELN"
     gene            complement(3051..3620)
                     /gene="ectA"
                     /locus_tag="NAI38-5751"
     CDS             complement(3051..3620)
                     /codon_start=1
                     /gene="ectA"
                     /locus_tag="NAI38-5751"
                     /product="L-2,4-diaminobutyric acid acetyltransferase"
                     /protein_id="QOE83930.1"
                     /transl_table=11
                     /translation="MTAAQADHAGARSDIREFPEGFKLDTPRVEDGAAIWRIARDSKAL
                     DLNSSYSYLLWCRDFAATSVVARDTEGEPAAFITGYLRPERPETLVVWQVAVDDAHRGQ
                     GLAAALLDGLTARARDELGVRFVETTVTPDNTASNRLFASFAERHSVPLKSEVLFDAGL
                     FPEQGHEPEVLHLIGPFEPPARSGHS"
ORIGIN
        1 tacaactcga cggacgacgt ggaccggttg ctggccctgc tggggtaggg ccggtccgac
       61 gggccggcaa cctgcgccgc cggccggcgc ccggcggccc ctccaacacc ccggaccggc
      121 cgtacaacac ggcgcggccg ccgcacagac cagctgtgcg gcggccgcgc caccggtgcg
      181 agtgtcccct aacgcaccgg agtgaagtcg cgggccccga tgtactccgg gcgccggacc
      241 ggcgctgcga acggctccac cgcagcgttc tccacgctgt tgaagacgat gaagacattg
      301 ctgcgcgggt agggcgtgat gttgtcgccc gagccgtgca tggcgttgca gtcgaaccac
      361 gtcgccgagc cggccttacc ggtgaagaga cggatgctgt gcttgtccgc catcttcgtg
      421 agcacctcgt ccgacgggat gcccgcgtcc tgcatctgca gcgaccgctt gtagttgtcc
      481 ttcggcgtcg caccctcaca gcccacgaag tactcgtgcg agccgggcat gatcatcaag
      541 ccgccgttgg tgtcgtaatt ctccgtcagc gcgatcgaga ccgacacggt gcgcatgttc
      601 ggcagaccgt cctcggcgtg ccaggtctcg aagtccgaat gccagtagaa gccggaggca
      661 ccgaagcccg gcttcacatt gatccgcgac tggtggacat agacgtccga gccgaggatc
      721 tgacgggcac ggccgaccac gcgcgggtcg gagaccagct tggcgaacac ctcgctgatc
      781 ttgtgcacct cgaacacggt ccgtacgtcc tgtgacttcg gctcgacgat ggagcgcggg
      841 tcggcgcgca tcgtcgggtc gaggacgaga cggtccagtt cggcgcggta caccgcgact
      901 tcttccgccg tgagcagttc cccgatggcg aaaaagccgt cgcgttcgaa gtcgctcagc
      961 tcggacggct cgaaggggcc ggccgttccg ggctgcgacc acaccaccgg gtccttccgc
     1021 ggggtgatga cctcagcggt cccacgggtc gggtacaggt cggcggtgcg ctcgggtgcg
     1081 gtggtcatgg tgttgccttc ctctcctctc gtacggaatc tggcttacgt gcgcgtgcgt
     1141 gtgccttcct cagcgctgcc ggacggggca ggcggatggc gcctcagacc gtctcgggct
     1201 cagactccgg ttcggtgaga agcgggtaga ccccgttctc gtcgtggtcc tcgcggccgg
     1261 tgacgggcgg gttgaagaca cacacgcagc ggaagtcctc cttgatccgc atggtgtgct
     1321 tctcgtgccc gtcgagcagg tacatcgtgc ccggcgtgat ggtgtacttc tcgccggtct
     1381 cgtcgttcgt gagctcggcc tcaccctcta cgcagagtac ggcctcgatg tggttcgcgt
     1441 accacatcga cgtctcggtc ccggcgtaca gggtggtctc gtgcagcgag aaaccgacgc
     1501 gctccttggc gagcacgatg cgcttgctct cccaggtgcc ggacttggcc ttgacgtggc
     1561 ggtcggtgcc ttcgatgtcc ttgaaggatc ggacgatcac ggtgactcgg tgcctttctg
     1621 tggaacggta aggggctctg gagtggtcag acgcagtcgc ggaccgcgcg ggcgaggatc
     1681 cggagtccct cgtccagctc ctcaggggtg gtcgtcagcg cgggcagcag cttgacgacc
     1741 tcgctctccg ggccggaggt ctcgatcagc agacccagct cgaaggcgcg cttggcaatc
     1801 ttgtcggcgg tgggcttgtc gttgagctcc atgccccaca ccagaccgcg gccgcggtac
     1861 tcggcgatgg cgtccgggtg ctcctcggtg atggccttga ggtgtgcttc gacgatctca
     1921 ccgcgggcga gagtctgctt ctccatctgg ccgtcggccc agtaggtgtc cagcgtcgcg
     1981 gcggcggtga cgaacgccgg gttgttgccg cggaaggtgc cgttgtgctc gcccggctcc
     2041 cagatgtcca gctcaggctt gaacagggtg agcgcgagcg gcaggccgta gccgctgatg
     2101 gacttcgaga cggtgacgat gtccggcacg atgcccgcct cctcgaagga gaagaaggca
     2161 cccgtacggc cgcaacccat ctggatgtcg tcgacgatga gcagcatgtc ccagcgctca
     2221 cacaggtcgg cgagggcacg cagccactcg ggacgggcga cgttgatgcc gccctcgccc
     2281 tggaccgtct cgacgatcac ggcggcgggg gtgttcagac cggagccctg gtcctccagc
     2341 agccgctcga accacaggaa gtccgggtag gtgccgtcga ggtagttgtc gaagggcatc
     2401 ggggtgccgt gcaccagcgg gataccggcg ccggcgcgct tcatggagtt accggtgacg
     2461 gccagcgcgc ccagggacat gccgtggaag gcgttggtga aggacacgat cgactcgcgg
     2521 cccttgacct tacgggccag cttcagcgcg gcctcgacgg agttggcgcc cgtcgggccc
     2581 gggaacatga ccttgtaggg caggtcacgg ggccgcagga tgatgttctg gaacgactcc
     2641 aggaacgccc gcttggccgt ggtggacatg tccaggccgt gggtgacgcc gtcccgctcg
     2701 atgtagtcga tcagggcgcg tttgaggacc gggttgttgt ggccgtagtt gagcgacccg
     2761 gcgccggcga agaagtcgag gtaggtgtgg ccgtcctcgt cggtcatgcg gctgccctgc
     2821 gcacggtcga agacggtggg ccagctacgg cagtagctgc ggacctcgga ctccacggtc
     2881 tcgaagacgc tcagatcggg ctgggtgatg gtcacagcat gctcctggga gatgagtggc
     2941 gaaaggatga gaagtctgta gggggctggg cggtaccggc ggtgtcacgg gggtgtggtg
     3001 ccgggcccgc atgaccgctg cggcgtcgcc gccgcgcggt cctgggtgcg tcaggagtgc
     3061 cccgagcggg ccggtggctc gaacgggccg atgaggtgca gcacctccgg ctcgtggccc
     3121 tgctccggga acagccccgc gtcgaagagc acctcgctct tgaggggcac tgagtgccgc
     3181 tcggcgaagg acgcgaacaa ccggttggat gcggtgttgt cgggggtcac ggtggtttcg
     3241 acgaaacgta cgcccagctc gtccctggcc cgggcggtca gcccgtccag gagtgcggcc
     3301 gccaggccct gcccacggtg cgcgtcgtcg acggcgacct gccagacgac gagggtctcc
     3361 gggcgctcgg ggcggaggta tccggtgatg aaggcggccg gctcgccctc cgtgtcgcgg
     3421 gcgacgacgg aggtggcggc gaagtcgcga caccacagga ggtagctgta cgaggagttg
     3481 agatccagcg ccttcgagtc gcgggcgatg cgccagatcg cggctccgtc ctccacgcgt
     3541 ggggtgtcga gcttgaagcc ctccggaaat tctctgatat cgcttcgggc acctgcatgg
     3601 tctgcttgtg cggcggtcat gggaattaaa tttacccagg gaaaagggaa attgcatcgc
     3661 gggcaggggt tacggaagtg agcgagatgt gttatcgcgc gggggcgccc gtgtgcgcga
     3721 tgccaccgcg agatgtcccc atttgaccag gaggttcggg gtgaaacgga cacgttgtgt
     3781 agtcggtcac agctccgtaa cgcccacgag atccgtccga attatggaac ttgccgctag
     3841 cgaaatcttg gcgtttgagg tgcggaaaag cgggcagaag aatacgggaa gctgtacttc
     3901 tgaggaattg ggaattcgcc gccggaaggc cgggcggaag gcccatggaa acctgcgcgg
     3961 caagaccgtg acaagacccg caagaagatg cgccaggcga tgtccgggaa ggctgcggga
     4021 aacgtacgga gagttgcgga gaacgcacgg agaaccgtgc agcacgggta gcggaagctg
//
