BGC0002055: mycalamide A biosynthetic gene cluster from sponge metagenome
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Location: 2,626 - 89,053 nt. (total: 86,428 nt).
This entry is originally from NCBI GenBank JAAHTG010000029.1, but has been modified (see Modifications tab for details).

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
Modifications
General information about the BGC
MIBiG accession BGC0002055
Short description mycalamide A biosynthetic gene cluster from sponge metagenome
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Trans-AT type I)
Loci
JAAHTG010000029.1
2626 - 89053
via
Compounds
  • mycalamide A
Species sponge metagenome [taxonomy]
References
Chemical products information
mycalamide A Evidence: NMR
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C24H41N1O10
Chemical database entries
PubCHEM
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • mycH
 2626 - 23382 (-) polyketide synthase
  • Scaffold biosynthesis
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  • mycG
 23395 - 24696 (-) mono-oxygenase
  • Scaffold biosynthesis
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  • mycF
 24757 - 52482 (-) polyketide synthase
  • Scaffold biosynthesis
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  • mycE
 52695 - 53504 (-) methyltransferase
  • Tailoring
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  • mycK2
 53595 - 54614 (-)
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    • mycT
    		 54861 - 55589 (-) di-oxygenase
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      • mycA
      		 55634 - 56680 (-) methyltransferase
      • Tailoring
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      • mycK1
      		 56832 - 57869 (+)
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        • mycJ
        		 58200 - 59327 (-) mono-oxygenase
        • Tailoring
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        • mycI
        		 59338 - 76785 (-) polyketide synthase
        • Scaffold biosynthesis
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        • mycB
        		 76835 - 78193 (-) enoyl reductase
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          • mycD
          		 78682 - 79809 (+) acyltransferase
          • Scaffold biosynthesis
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          • mycS
          		 80158 - 81027 (+)
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            • mycR
            		 81544 - 83205 (+) transporter
            • Transport
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            • mycQ
            		 83202 - 84242 (+)
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              • mycC
              		 84374 - 85378 (-) acyltransferase
              • Scaffold biosynthesis
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              • mycL
              		 85477 - 86259 (-) enoyl-CoA hydratase
              • Scaffold biosynthesis
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              • mycP
              		 86256 - 87521 (-) 3-hydroxy-3-methylglutaryl-CoA synthase
              • Scaffold biosynthesis
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              • mycM
              		 87580 - 88827 (-) ketosynthase
              • Scaffold biosynthesis
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              • mycN
              		 88814 - 89053 (-) acyl carrier protein
              • Scaffold biosynthesis
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              Biosynthesis information

              Biosynthetic modules

              Name
              16
              Type
              nrps-type1
              Genes
              mycH
              Substrates
              arginine (evidence: Sequence-based prediction)
              Integrated Monomers
              Domains
              condensation (LCL), adenylation
              Name
              6
              Type
              nrps-type1
              Genes
              mycF
              Substrates
              glycine (evidence: Structure-based inference [2])
              Integrated Monomers
              Domains
              condensation (LCL), adenylation
              Name
              1
              Type
              pks-trans-at
              Genes
              mycM
              Substrates
              Integrated Monomers
              Domains
              ketosynthase
              Name
              2
              Type
              pks-trans-at
              Genes
              mycI
              Substrates
              Integrated Monomers
              Domains
              ketosynthase, ketoreductase, methyltransferase, carrier (ACP), carrier (ACP)
              Name
              3
              Type
              pks-trans-at
              Genes
              mycI
              Substrates
              Integrated Monomers
              Domains
              ketosynthase, other (Enoyl-CoA dehydratase), carrier (ACP), carrier (ACP), carrier (ACP)
              Name
              4
              Type
              pks-trans-at
              Genes
              mycI
              Substrates
              Integrated Monomers
              Domains
              ketosynthase, ketoreductase, carrier (ACP)
              Name
              5
              Type
              pks-trans-at
              Genes
              mycF
              Substrates
              Integrated Monomers
              Domains
              ketosynthase, carrier (ACP)
              Name
              7
              Type
              pks-trans-at
              Genes
              mycF
              Substrates
              Integrated Monomers
              Domains
              ketosynthase, ketoreductase, carrier (ACP)
              Name
              8
              Type
              pks-trans-at
              Genes
              mycF
              Substrates
              Integrated Monomers
              Domains
              ketosynthase, ketoreductase, methyltransferase, carrier (ACP), carrier (ACP)
              Name
              9
              Type
              pks-trans-at
              Genes
              mycF
              Substrates
              Integrated Monomers
              Domains
              ketosynthase, dehydratase, ketoreductase, other (Pyran synthase), carrier (ACP)
              Name
              10
              Type
              pks-trans-at
              Genes
              mycH
              Substrates
              Integrated Monomers
              Domains
              ketosynthase, ketoreductase, carrier (ACP)
              Name
              11
              Type
              pks-trans-at
              Genes
              mycH
              Substrates
              Integrated Monomers
              Domains
              ketosynthase
              Name
              12
              Type
              pks-trans-at
              Genes
              mycH
              Substrates
              Integrated Monomers
              Domains
              ketosynthase, dehydratase, ketoreductase, carrier (ACP), carrier (ACP), carrier (ACP)
              Name
              13
              Type
              pks-trans-at
              Genes
              mycH
              Substrates
              Integrated Monomers
              Domains
              ketosynthase, dehydratase, carrier (ACP)
              Name
              14
              Type
              pks-trans-at
              Genes
              mycH
              Substrates
              Integrated Monomers
              Domains
              ketosynthase, dehydratase, ketoreductase, carrier (ACP)
              Name
              15
              Type
              pks-trans-at
              Genes
              mycH
              Substrates
              Integrated Monomers
              Domains
              ketosynthase, carrier (ACP)
              Annotation changelog

              Entry version: 2

              Date
              Changes
              Submitters
              Reviewers
              2022-10-07
              Update chemical activity to schema version 2.11
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              2022-10-07
              Changed chemical structures for compounds: mycalamide A.
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              2022-10-07
              Added NPAtlas links to compounds: mycalamide A.
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

              Entry version: 1

              Date
              Changes
              Submitters
              Reviewers
              2022-09-15
              Submitted
              • (ID: DSCNCSJNYVZZHSG74Z2O37QE)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              2022-09-15
              Removed ketoreductase stereochemistry annotation from modules without ketoreductases
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              2022-09-15
              Sorted modules by module number
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              2022-09-15
              Removed gene annotations with no information
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              2022-09-15
              Removed additional gene with no identifier or information
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              2022-09-15
              Fixed biosynthetic classes
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              2022-09-15
              Updated NRP substrate specificities
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              Detailed domain annotation
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              Modifications to original record
              • mycH was added
              • mycG was added
              • mycF was added
              • mycE was added
              • mycK2 was added
              • mycT was added
              • mycA was added
              • mycK1 was added
              • mycJ was added
              • mycI was added
              • mycB was added
              • mycD was added
              • mycS was added
              • mycR was added
              • mycQ was added
              • mycC was added
              • mycL was added
              • mycP was added
              • mycM was added
              • mycN was added