BGC0002055: mycalamide A biosynthetic gene cluster from sponge metagenome
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Location: 2,626 - 89,053 nt. (total: 86,428 nt).
This entry is originally from NCBI GenBank JAAHTG010000029.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
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Gene details
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General
Compounds
Genes
Polyketide
NRP
History
KnownClusterBlast
NRPS/PKS domains
General information about the BGC
MIBiG accession BGC0002055
Short description mycalamide A biosynthetic gene cluster from sponge metagenome
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • Polyketide
Loci NCBI GenBank: JAAHTG010000029.1
Compounds
  • mycalamide A
Species sponge metagenome [taxonomy]
References
Chemical products information
mycalamide A
Copy SMILES
C24H41N1O10
Chemical database entries
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • mycH
2626 - 23382 (-) polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • mycG
23395 - 24696 (-) mono-oxygenase
  • Scaffold biosynthesis
Sequence-based prediction
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  • mycF
24757 - 52482 (-) polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • mycE
52695 - 53504 (-) methyltransferase
  • Tailoring (Methylation)
Sequence-based prediction
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  • mycK2
53595 - 54614 (-) Unknown
  • Unknown
Sequence-based prediction
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  • No_gene_ID
54692 - 54874 (-)
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  • mycT
54861 - 55589 (-) di-oxygenase
  • Unknown
Sequence-based prediction
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  • mycA
55634 - 56680 (-) methyltransferase
  • Tailoring (Methylation)
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  • mycK1
56832 - 57869 (+) Unknown
  • Unknown
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  • mycJ
58200 - 59327 (-) mono-oxygenase
  • Tailoring (Oxidation)
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  • mycI
59338 - 76785 (-) polyketide synthase
  • Scaffold biosynthesis
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  • mycB
76835 - 78193 (-) enoyl reductase
  • Unknown
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  • mycD
78682 - 79809 (+) acyltransferase
  • Scaffold biosynthesis
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  • mycS
80158 - 81027 (+) Unknown
  • Unknown
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  • mycR
81544 - 83205 (+) transporter
  • Transport
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  • mycQ
83202 - 84242 (+) Unknown
  • Unknown
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  • mycC
84374 - 85378 (-) acyltransferase
  • Scaffold biosynthesis
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  • mycL
85477 - 86259 (-) enoyl-CoA hydratase
  • Scaffold biosynthesis
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  • mycP
86256 - 87521 (-) 3-hydroxy-3-methylglutaryl-CoA synthase
  • Scaffold biosynthesis
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  • mycM
87580 - 88827 (-) ketosynthase
  • Scaffold biosynthesis
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  • mycN
88814 - 89053 (-) acyl carrier protein
  • Scaffold biosynthesis
Sequence-based prediction
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  • No gene ID
  • No gene ID
(external) Unknown
  • Unknown
Sequence-based prediction
  • No gene ID
  • No gene ID
(external) Unknown
  • Unknown
Sequence-based prediction
  • No gene ID
  • No gene ID
(external) Unknown
  • Unknown
Sequence-based prediction
  • No gene ID
  • No gene ID
(external) Unknown
  • Unknown
Sequence-based prediction
Polyketide-specific information
Subclass Unknown
Starter unit Acetyl-CoA
Cyclic? no
Polyketide-synthases
Genes Properties Modules
mycF
+
mycH
+
mycM
+
mycI
Synthase subclass: Trans-AT type I
Module 1
Genes: mycM
Core domains: Ketosynthase
Scaffold-modifying domains: Beta-branching
Module 10
Genes: mycH
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
Module 11
oxygen insertion

Genes: mycH
Core domains: Ketosynthase
Module 12
Genes: mycH
Core domains: Thiolation (ACP/PCP), Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP), Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 13
Genes: mycH
Core domains: Ketosynthase, Dehydratase, Thiolation (ACP/PCP)
Module 14
Genes: mycH
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 15
Genes: mycH
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module 2
Genes: mycI
Core domains: Thiolation (ACP/PCP), Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
Module 3
Genes: mycI
Core domains: Ketosynthase, Thiolation (ACP/PCP), Thiolation (ACP/PCP), Thiolation (ACP/PCP)
Scaffold-modifying domains: Crotonase / Enoyl-CoA dehydratase
Module 4
Genes: mycI
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
Module 5
Genes: mycF
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module 7
Genes: mycF
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
Module 8
Genes: mycF
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP), Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: D-OH
Module 9
Genes: mycF
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Pyran synthase
KR-domain stereochemistry: D-OH
NRP-specific information
Subclass n/a
Cyclic? no
NRP-synthases
Gene Modules
mycH
Module 16
Specificity: Arginine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
mycF
Module 6
Specificity: Glycine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
Annotation changelog
MIBiG version Submitter Notes
2.1
  • Hidden contributor (ID: DSCNCSJNYVZZHSG74Z2O37QE, no GDPR consent given).
  • Submitted
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