BGC0002055: mycalamide A biosynthetic gene cluster from sponge metagenome
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Location: 2,626 - 89,053 nt. (total: 86,428 nt).
This entry is originally from NCBI GenBank JAAHTG010000029.1, but has been modified (see Modifications tab for details).

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
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General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
Modifications
General information about the BGC
MIBiG accession BGC0002055
Short description mycalamide A biosynthetic gene cluster from sponge metagenome
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP
  • Polyketide
Loci NCBI GenBank: JAAHTG010000029.1
Compounds
  • mycalamide A
Species sponge metagenome [taxonomy]
References
Chemical products information
mycalamide A
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C24H41N1O10
Chemical database entries
PubCHEM
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • mycH
 2626 - 23382 (-) polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • mycG
 23395 - 24696 (-) mono-oxygenase
  • Scaffold biosynthesis
Sequence-based prediction
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  • mycF
 24757 - 52482 (-) polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • mycE
 52695 - 53504 (-) methyltransferase
  • Tailoring (Methylation)
Sequence-based prediction
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  • mycK2
 53595 - 54614 (-)
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  • mycT
 54861 - 55589 (-) di-oxygenase
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  • mycA
 55634 - 56680 (-) methyltransferase
  • Tailoring (Methylation)
Sequence-based prediction
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  • mycK1
 56832 - 57869 (+)
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  • mycJ
 58200 - 59327 (-) mono-oxygenase
  • Tailoring (Oxidation)
Sequence-based prediction
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  • mycI
 59338 - 76785 (-) polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • mycB
 76835 - 78193 (-) enoyl reductase
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  • mycD
 78682 - 79809 (+) acyltransferase
  • Scaffold biosynthesis
Sequence-based prediction
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  • mycS
 80158 - 81027 (+)
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  • mycR
 81544 - 83205 (+) transporter
  • Transport
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  • mycQ
 83202 - 84242 (+)
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  • mycC
 84374 - 85378 (-) acyltransferase
  • Scaffold biosynthesis
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  • mycL
 85477 - 86259 (-) enoyl-CoA hydratase
  • Scaffold biosynthesis
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  • mycP
 86256 - 87521 (-) 3-hydroxy-3-methylglutaryl-CoA synthase
  • Scaffold biosynthesis
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  • mycM
 87580 - 88827 (-) ketosynthase
  • Scaffold biosynthesis
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  • mycN
 88814 - 89053 (-) acyl carrier protein
  • Scaffold biosynthesis
Sequence-based prediction
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Polyketide-specific information
Subclass Unknown
Starter unit Acetyl-CoA
Cyclic? no
Polyketide-synthases
Genes Properties Modules
mycF
+
mycH
+
mycM
+
mycI 		Synthase subclass: Trans-AT type I
Module 1
Genes: mycM
Core domains: Ketosynthase
Scaffold-modifying domains: Beta-branching
Module 2
Genes: mycI
Core domains: Thiolation (ACP/PCP), Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: Unknown
Module 3
Genes: mycI
Core domains: Ketosynthase, Thiolation (ACP/PCP), Thiolation (ACP/PCP), Thiolation (ACP/PCP)
Scaffold-modifying domains: Crotonase / Enoyl-CoA dehydratase
Module 4
Genes: mycI
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 5
Genes: mycF
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module 7
Genes: mycF
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 8
Genes: mycF
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP), Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: D-OH
Module 9
Genes: mycF
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Pyran synthase
KR-domain stereochemistry: D-OH
Module 10
Genes: mycH
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 11
oxygen insertion

Genes: mycH
Core domains: Ketosynthase
Module 12
Genes: mycH
Core domains: Thiolation (ACP/PCP), Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP), Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 13
Genes: mycH
Core domains: Ketosynthase, Dehydratase, Thiolation (ACP/PCP)
Module 14
Genes: mycH
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 15
Genes: mycH
Core domains: Ketosynthase, Thiolation (ACP/PCP)
NRP-specific information
Subclass N/A
Cyclic? no
NRP-synthases
Gene Modules
mycH
Module 16 [inactive]
Specificity: arginine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
mycF
Module 6 [inactive]
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Annotation changelog
MIBiG version Submitter Notes
3.0
  • Hidden contributor (ID: DSCNCSJNYVZZHSG74Z2O37QE, no GDPR consent given).
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Submitted
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Sorted modules by module number
  • Removed gene annotations with no information
  • Removed additional gene with no identifier or information
  • Fixed biosynthetic classes
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
  • Changed chemical structures for compounds: mycalamide A.
  • Added NPAtlas links to compounds: mycalamide A.
Detailed domain annotation
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Modifications to original record
  • mycH was added
  • mycG was added
  • mycF was added
  • mycE was added
  • mycK2 was added
  • mycT was added
  • mycA was added
  • mycK1 was added
  • mycJ was added
  • mycI was added
  • mycB was added
  • mycD was added
  • mycS was added
  • mycR was added
  • mycQ was added
  • mycC was added
  • mycL was added
  • mycP was added
  • mycM was added
  • mycN was added