BGC0002059: spliceostatin L biosynthetic gene cluster from Xanthomonas cannabis pv. cannabis
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Location: 1 - 72,874 nt. (total: 72,874 nt).
This entry is originally from NCBI GenBank BK010647.1.

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core biosynthetic genes
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General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0002059
Short description spliceostatin L biosynthetic gene cluster from Xanthomonas cannabis pv. cannabis
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP
  • Polyketide (Other)
Loci NCBI GenBank: BK010647.1
Compounds
  • spliceostatin L
  • spliceostatin M
Species Xanthomonas cannabis pv. cannabis [taxonomy]
References
Chemical products information
spliceostatin L
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C29H45N1O8
Chemical database entries
NPAtlas
spliceostatin M
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C28H43N1O8
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • DAD54482.1
  • sxcA
 1 - 171 (-) transposase
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  • DAD54483.1
  • sxcB
 257 - 940 (-) GCN5-related N-acetyltransferase
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  • DAD54484.1
  • sxcC
 1475 - 1741 (+) transposase
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  • DAD54485.1
  • sxcD
 2668 - 17694 (+) trans-acyltransferase polyketide synthase
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  • DAD54486.1
  • sxcE
 17672 - 42346 (+) trans-acyltransferase polyketide synthase
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  • DAD54487.1
  • sxcF
 42404 - 56938 (+) trans-acyltransferase polyketide synthase
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  • DAD54488.1
  • sxcG
 56959 - 62853 (+) trans-acyltransferase polyketide synthase
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  • DAD54489.1
  • sxcH
 62923 - 63840 (+) acyltransferase
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  • DAD54490.1
  • sxcI
 63907 - 64371 (+) hypothetical protein
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  • DAD54491.1
  • sxcJ
 65711 - 66970 (+) hydroxymethylglutaryl-coenzyme A synthase
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  • DAD54492.1
  • sxcK
 66958 - 67743 (+) enyl-CoA hydratase
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  • DAD54493.1
  • sxcL
 67832 - 68071 (+) acyl carrier protein
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  • DAD54494.1
  • sxcM
 68064 - 69344 (+) polyketide beta-ketoacyl:ACP synthase
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  • DAD54495.1
  • sxcN
 69341 - 70474 (+) [acyl-carrier-protein] S-malonyltransferase
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  • DAD54496.1
  • sxcO
 70578 - 71492 (+) [acyl-carrier-protein] S-malonyltransferase
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  • DAD54497.1
  • sxcP
 71509 - 72309 (+) hypothetical protein
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Polyketide-specific information
Subclass Other
Cyclic? no
Polyketide-synthases
Genes Properties Modules
sxcO
+
sxcN
+
sxcD
+
sxcF
+
sxcH
+
sxcG
+
sxcE 		Synthase subclass: Trans-AT type I
Trans-acyltansferases: sxcH, sxcN, sxcO
Module 0
Genes: sxcD
Core domains: Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: FkbH
KR-domain stereochemistry: Unknown
Module 1
Genes: sxcD
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 2
Genes: sxcD
Core domains: Ketosynthase, Thiolation (ACP/PCP), Thiolation (ACP/PCP), Thioesterase
Module 3
Genes: sxcD
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module 5
Genes: sxcE
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP), Thiolation (ACP/PCP), Thiolation (ACP/PCP), Enoylreductase
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: Unknown
Module 6
Genes: sxcE
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP), Thiolation (ACP/PCP)
Scaffold-modifying domains: Pyran synthase
KR-domain stereochemistry: Unknown
Module 7
Genes: sxcE
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: Unknown
Module 8
Genes: sxcE, sxcF
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 9
Genes: sxcF
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 10
Genes: sxcF
Core domains: Ketosynthase, Thiolation (ACP/PCP), Thiolation (ACP/PCP)
Scaffold-modifying domains: Crotonase / Enoyl-CoA dehydratase
Module 11
Genes: sxcF, sxcG
Core domains: Ketosynthase, Thiolation (ACP/PCP), Thiolation (ACP/PCP)
Scaffold-modifying domains: Oxidation
Module 12
Genes: sxcG
Core domains: Ketosynthase, Dehydratase, Thiolation (ACP/PCP)
Module 13
Genes: sxcG
Core domains: Thioesterase
NRP-specific information
Subclass N/A
Cyclic? no
NRP-synthases
Gene Modules
sxcD
Module 4
catalyzes condensation of amino acid and polyketide
sxcE
Module 4
catalyzes condensation of amino acid and polyketide

Specificity: threonine
Evidence for specificity:
  • Structure-based inference
  • Homology
Annotation changelog
MIBiG version Submitter Notes
3.0
  • Hidden contributor (ID: RLTUJMQB5UUY7AZHW5PDUHV4, no GDPR consent given).
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Submitted
  • Corrected NRP module activity
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Merged cross-CDS PKS modules
  • Sorted modules by module number
  • Added version to NCBI accession
  • Corrected NRP gene names
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
  • Changed chemical structures for compounds: spliceostatin L, spliceostatin M.
  • Added NPAtlas links to compounds: spliceostatin L, spliceostatin M.
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