BGC0002059: spliceostatin L biosynthetic gene cluster from Xanthomonas cannabis pv. cannabis
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Location: 1 - 72,874 nt. (total: 72,874 nt).
This entry is originally from NCBI GenBank BK010647.1.

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core biosynthetic genes
additional biosynthetic genes
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0002059
Short description spliceostatin L biosynthetic gene cluster from Xanthomonas cannabis pv. cannabis
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Trans-AT type I)
Loci
BK010647.1
via
Compounds
  • spliceostatin L
  • spliceostatin M
Species Xanthomonas cannabis pv. cannabis [taxonomy]
References
Chemical products information
spliceostatin L Evidence: NMR, Mass spectrometry
Copy SMILES
C29H45N1O8
Chemical database entries
NPAtlas
spliceostatin M Evidence: NMR, Mass spectrometry
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C28H43N1O8
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • DAD54482.1
  • sxcA
 1 - 171 (-) transposase
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    • DAD54483.1
    • sxcB
    		 257 - 940 (-) GCN5-related N-acetyltransferase
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      • DAD54484.1
      • sxcC
      		 1475 - 1741 (+) transposase
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        • DAD54485.1
        • sxcD
        		 2668 - 17694 (+) trans-acyltransferase polyketide synthase
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          • DAD54486.1
          • sxcE
          		 17672 - 42346 (+) trans-acyltransferase polyketide synthase
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            • DAD54487.1
            • sxcF
            		 42404 - 56938 (+) trans-acyltransferase polyketide synthase
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              • DAD54488.1
              • sxcG
              		 56959 - 62853 (+) trans-acyltransferase polyketide synthase
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                • DAD54489.1
                • sxcH
                		 62923 - 63840 (+) acyltransferase
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                  • DAD54490.1
                  • sxcI
                  		 63907 - 64371 (+) hypothetical protein
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                    • DAD54491.1
                    • sxcJ
                    		 65711 - 66970 (+) hydroxymethylglutaryl-coenzyme A synthase
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                      • DAD54492.1
                      • sxcK
                      		 66958 - 67743 (+) enyl-CoA hydratase
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                        • DAD54493.1
                        • sxcL
                        		 67832 - 68071 (+) acyl carrier protein
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                          • DAD54494.1
                          • sxcM
                          		 68064 - 69344 (+) polyketide beta-ketoacyl:ACP synthase
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                            • DAD54495.1
                            • sxcN
                            		 69341 - 70474 (+) [acyl-carrier-protein] S-malonyltransferase
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                              • DAD54496.1
                              • sxcO
                              		 70578 - 71492 (+) [acyl-carrier-protein] S-malonyltransferase
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                                • DAD54497.1
                                • sxcP
                                		 71509 - 72309 (+) hypothetical protein
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                                  Biosynthesis information

                                  Biosynthetic modules

                                  Name
                                  4
                                  Type
                                  nrps-type1
                                  Genes
                                  sxcE
                                  Substrates
                                  threonine (evidence: Structure-based inference, Homology)
                                  Integrated Monomers
                                  Domains
                                  adenylation
                                  Name
                                  0
                                  Type
                                  pks-trans-at-starter
                                  Genes
                                  sxcD
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  dehydratase, ketoreductase, other (FkbH), carrier (ACP)
                                  Name
                                  1
                                  Type
                                  pks-trans-at
                                  Genes
                                  sxcD
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, ketoreductase, carrier (ACP)
                                  Name
                                  2
                                  Type
                                  pks-trans-at
                                  Genes
                                  sxcD
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, thioesterase, carrier (ACP), carrier (ACP)
                                  Name
                                  3
                                  Type
                                  pks-trans-at
                                  Genes
                                  sxcD
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, carrier (ACP)
                                  Name
                                  5
                                  Type
                                  pks-trans-at
                                  Genes
                                  sxcE
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, dehydratase, ketoreductase, enoylreductase, methyltransferase, carrier (ACP), carrier (ACP), carrier (ACP)
                                  Name
                                  6
                                  Type
                                  pks-trans-at
                                  Genes
                                  sxcE
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, dehydratase, ketoreductase, other (Pyran synthase), carrier (ACP), carrier (ACP)
                                  Name
                                  7
                                  Type
                                  pks-trans-at
                                  Genes
                                  sxcE
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, dehydratase, ketoreductase, methyltransferase, carrier (ACP)
                                  Name
                                  8
                                  Type
                                  pks-trans-at
                                  Genes
                                  sxcE sxcF
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, dehydratase, ketoreductase, carrier (ACP)
                                  Name
                                  9
                                  Type
                                  pks-trans-at
                                  Genes
                                  sxcF
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, ketoreductase, carrier (ACP)
                                  Name
                                  10
                                  Type
                                  pks-trans-at
                                  Genes
                                  sxcF
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, other (Enoyl-CoA dehydratase), carrier (ACP), carrier (ACP)
                                  Name
                                  11
                                  Type
                                  pks-trans-at
                                  Genes
                                  sxcF sxcG
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, oxidase, carrier (ACP), carrier (ACP)
                                  Name
                                  12
                                  Type
                                  pks-trans-at
                                  Genes
                                  sxcG
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  ketosynthase, dehydratase, carrier (ACP)
                                  Name
                                  13
                                  Type
                                  pks-trans-at-starter
                                  Genes
                                  sxcG
                                  Substrates
                                  Integrated Monomers
                                  Domains
                                  thioesterase
                                  Annotation changelog

                                  Entry version: 2

                                  Date
                                  Changes
                                  Submitters
                                  Reviewers
                                  2022-10-07
                                  Update chemical activity to schema version 2.11
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  2022-10-07
                                  Changed chemical structures for compounds: spliceostatin L, spliceostatin M.
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  2022-10-07
                                  Added NPAtlas links to compounds: spliceostatin L, spliceostatin M.
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                  Entry version: 1

                                  Date
                                  Changes
                                  Submitters
                                  Reviewers
                                  2022-09-15
                                  Submitted
                                  • (ID: RLTUJMQB5UUY7AZHW5PDUHV4)
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  2022-09-15
                                  Corrected NRP module activity
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  2022-09-15
                                  Removed ketoreductase stereochemistry annotation from modules without ketoreductases
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  2022-09-15
                                  Merged cross-CDS PKS modules
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  2022-09-15
                                  Sorted modules by module number
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  2022-09-15
                                  Added version to NCBI accession
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  2022-09-15
                                  Corrected NRP gene names
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  2022-09-15
                                  Updated NRP substrate specificities
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                  Detailed domain annotation
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