BGC0002061: nostopeptolide A1 biosynthetic gene cluster from Nostoc punctiforme PCC 73102
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Location: 2,667,205 - 2,708,401 nt. (total: 41,197 nt).
This entry is originally from NCBI GenBank NC_010628.1, but has been modified (see Modifications tab for details).

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
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Gene details
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General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
Modifications
General information about the BGC
MIBiG accession BGC0002061
Short description nostopeptolide A1 biosynthetic gene cluster from Nostoc punctiforme PCC 73102
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP (Cyclic depsipeptide)
  • Polyketide (Other)
Loci NCBI GenBank: NC_010628.1
Compounds
  • nostopeptolide A1
  • nostopeptolide 1052
Species Nostoc punctiforme PCC 73102 [taxonomy]
References
Chemical products information
nostopeptolide A1
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C53H80N10O14
Chemical database entries
PubCHEM
NPAtlas
nostopeptolide 1052
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List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • NPUN_RS10970
  • WP_012408783.1
 2667205 - 2680374 (+) non-ribosomal peptide synthetase
  • Scaffold biosynthesis
Sequence-based prediction
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  • NPUN_RS10975
  • WP_012408784.1
 2680371 - 2684147 (+) acyltransferase domain-containing protein
  • Scaffold biosynthesis
Sequence-based prediction
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  • NPUN_RS40615
  • WP_012408785.1
 2684199 - 2695850 (+) non-ribosomal peptide synthetase
  • Scaffold biosynthesis
Sequence-based prediction
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  • NPUN_RS40620
  • WP_012408786.1
 2695847 - 2703454 (+) non-ribosomal peptide synthetase
  • Scaffold biosynthesis
Sequence-based prediction
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  • NPUN_RS10990
  • WP_012408787.1
 2703494 - 2704291 (+) hypothetical protein
  • Unknown
Sequence-based prediction
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  • NPUN_RS10995
  • WP_012408788.1
 2704340 - 2705446 (+) zinc-binding dehydrogenase
  • Precursor biosynthesis
Sequence-based prediction
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  • NPUN_RS11000
  • WP_012408789.1
  • proC
 2705486 - 2706307 (+) pyrroline-5-carboxylate reductase
  • Precursor biosynthesis
Sequence-based prediction
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  • NPUN_RS11005
  • WP_012408790.1
 2706410 - 2708401 (+) ABC transporter ATP-binding protein/permease
  • Transport
Sequence-based prediction
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Polyketide-specific information
Subclass Other
Cyclic? yes
Polyketide-synthases
Genes Properties Modules
NPUN_RS10975 Synthase subclass: Modular type I
Module 5
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: NPUN_RS10975
Core domains: Ketosynthase, Acyltransferase, Thiolation (ACP/PCP)
NRP-specific information
Subclass Cyclic depsipeptide
Cyclic? yes
Release type
  • Macrolactonization
NRP-synthases
Gene Modules
NPUN_RS10970
Module 1
Specificity: isoleucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 2
Specificity: serine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 3
Specificity: 4S-methylproline
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 4
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
NPUN_RS40615
Module 6
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 7
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 8
Specificity: asparagine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: DCL
NPUN_RS40620
Module 10
Specificity: tyrosine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 9
Specificity: proline
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Annotation changelog
MIBiG version Submitter Notes
3.0
  • Hidden contributor (ID: KTTDKEJDYAQ7MKSNPMBDOYYX, no GDPR consent given).
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Submitted
  • Corrected NRP module activity
  • Removed gene names of 'No gene ID'
  • Corrected duplicate gene name
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Added version to NCBI accession
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
  • Changed chemical structures for compounds: nostopeptolide A1.
  • Added NPAtlas links to compounds: nostopeptolide A1.
Detailed domain annotation
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Modifications to original record
  • NPUN_RS00010 crossed the origin and was split into two features