BGC0002069: styrolide A biosynthetic gene cluster from Pseudomonas fluorescens
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Location: 26,882 - 37,505 nt. (total: 10,624 nt).
This entry is originally from NCBI GenBank VCNJ01000015.1.

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General
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General information about the BGC
MIBiG accession BGC0002069
Short description styrolide A biosynthetic gene cluster from Pseudomonas fluorescens
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Remarks "The styrolide BGC spans locus 1-9. However, stoR (locus 10, the single gene on another contig, not located in the proximity of the BGC) was identified through protein homology search based on acaR. The acaterin cluster aca will also be submitted to MIBiG. Due to high reactivity of styrolide B, styrolide A forms also upon heterologous expression of stoA-stoI, without stoR (and also in the respective stoR gene deletion mutant). Since formation of the styrolide A-related acaterin requires acaR, we wish to include the homologous enzyme stoR (84% seq. identity) for the styrolide BGC entry, since it is plausible that this gene product contributes to the formation of the final product of the pathway, styrolide A. \nGene deletion mutants verified essential genes stoB, C, D, E and G. Compound production in the heterologous host using the essential set of genes was not possible. Instead, stoA-stoI was required for compound production in the heterologous host. We hypothesize that the native producer holds alternative pathways/enzymes to compensate for gene deletions of stoA, stoF, stoH, and stoI, thus allowing compound production in these gene deletion mutants."
Biosynthetic class(es)
  • Other (Aminocoumarin)
Loci NCBI GenBank: VCNJ01000015.1
Compounds
  • styrolide A
  • styrolide B
Species Pseudomonas fluorescens [taxonomy]
References
Chemical products information
styrolide A
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C13H12O3
Chemical database entries
NPAtlas
styrolide B
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C13H10O3
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • FGA82_11240
  • TMU80018.1
 26882 - 30532 (+) indolepyruvate ferredoxin oxidoreductase family protein
  • Precursor biosynthesis
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  • FGA82_11245
  • TMU80019.1
 30596 - 31660 (+) A-factor biosynthesis protein
  • Scaffold biosynthesis
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  • FGA82_11250
  • TMU80020.1
 31657 - 32838 (+) NADH:flavin oxidoreductase
  • Tailoring (Dehydration)
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  • FGA82_11255
  • TMU80021.1
 32835 - 33608 (+) SDR family oxidoreductase
  • Scaffold biosynthesis
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  • FGA82_11260
  • TMU80022.1
 33608 - 34426 (+) SDR family NAD(P)-dependent oxidoreductase
  • Tailoring (Dehydration)
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  • FGA82_11265
  • TMU80023.1
 34468 - 34782 (+) hypothetical protein
  • Unknown
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  • FGA82_11270
  • TMU80024.1
 34801 - 36156 (+) long-chain fatty acid--CoA ligase
  • Precursor biosynthesis
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  • FGA82_11275
  • TMU80025.1
 36193 - 36435 (+) acyl carrier protein
  • Precursor biosynthesis
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  • FGA82_11280
  • TMU80026.1
 36432 - 37505 (+) ketoacyl-ACP synthase III
  • Precursor biosynthesis
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Annotation changelog
MIBiG version Submitter Notes
3.0
  • Hidden contributor (ID: 6CADHILFODRMCII55WHSHZ6V, no GDPR consent given).
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Submitted
  • Added version to NCBI accession
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
  • Changed chemical structures for compounds: styrolide B, styrolide A.
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