LOCUS       BGC0002074              2630 bp    DNA     linear   BCT 29-NOV-2019
DEFINITION  Streptomyces huasconensis strain HST28 huascopeptin biosynthetic
            gene cluster, complete sequence.
ACCESSION   BGC0002074
VERSION     BGC0002074
KEYWORDS    .
SOURCE      Streptomyces huasconensis
  ORGANISM  Streptomyces huasconensis
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-08 06:33:23
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2630
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            1..135
                     /gene="hptA"
     CDS             1..135
                     /codon_start=1
                     /gene="hptA"
                     /product="lasso peptide precursor"
                     /protein_id="QCY49166.1"
                     /transl_table=11
                     /translation="MYEQELKEEFTAQDYVPPMLAEVGEFSEDTLGYGNAWDSKNGLF"
     source          1..2630
                     /country="Chile"
                     /db_xref="taxon:1854574"
                     /isolation_source="arid soil sample"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces huasconensis"
                     /strain="HST28"
                     /type_material="type strain of Streptomyces huasconensis"
     misc_feature    1..2630
                     /note="huascopeptin biosynthetic gene cluster"
     subregion       1..2630
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="RiPP"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..2630
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            150..1991
                     /gene="hptC"
     CDS             150..1991
                     /codon_start=1
                     /gene="hptC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177:asparagine synthase (glutamine-hydrolyzing)
                     (Score: 114.5; E-value: 8.6e-35)"
                     /gene_kind="biosynthetic"
                     /note="lasso peptide cyclase"
                     /product="lasso peptide cyclase"
                     /protein_id="QCY49167.1"
                     /sec_met_domain="Asn_synthase (E-value: 9e-40, bitscore:
                     125.4, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIDTGYVVLPDHSAAENAIGSTAARFPLVASHDSGRPWLLGRRPA
                     GQVVVATKGPVRVAVIGACPVTAAQLGRFASYVRSLSDVGTLARKLPGSSILAVSVHGQ
                     TRVQGSIVGLHPVFHARVRGVDVAADRADLLAAMADASIDEESLAVRVACGRLLPAPLS
                     HRPLWGGVSALSPDHCLVWEKERAREMRWWAPPAPEQSLGEGAGKVREVMAAAMAHRAP
                     EEGRLSTDLSGGMDSTSLCFLAARKTPDILTFRTAEADVTNDDALFASHSISALPAAEH
                     MVAPQEDLPSIFTDPGSWASTEEPYPFIRTGAQTRYVARLLAEHGSRRHLAGHGGDELF
                     TSLAGYLHELLLRRPGTAFRHVRGYSALKRWPLAETAAALLRPGTVRSWWQDQATHLTA
                     PPPPLRRPVLGWGLWPLRASPWVTETANDLARTAFLRTAVETPRYAENLGQHQLLLILR
                     SSAFLHRLLREPFARESIHLDMPFFDDRLVEAVLAVRPEERSDPWRYKPLLSEAMRGVL
                     PDMVLGRSTKGEFSEDVRIGMKGNVPALLDVFADSRLAARGLIDADRLRSSLLAPQRNT
                     TILQDIEYLLGCESWLRAVEASTPTHSGEPCVHPSAP"
     gene            1969..2229
                     /gene="hptB1"
     CDS             1969..2229
                     /codon_start=1
                     /gene="hptB1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Lasso_Fused_RRE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /note="PqqD; RiPP recognition element; RRE"
                     /product="RiPP recognition element"
                     /protein_id="QCY49168.1"
                     /sec_met_domain="PF05402 (E-value: 2.1e-14, bitscore: 41.8,
                     seeds: 461, tool: rule-based-clusters)"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 5.7e-27,
                     bitscore: 81.9, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="Lasso_Fused_RRE (E-value: 1.5e-12,
                     bitscore: 35.7, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIRLRPDVSLAETPDGTVLLDQAKGRYWQLNATGARILRRLLDG
                     HSPEEIARAIAAGCGIGLDRALRDVDLVTEQLDRAGLAVSR"
     gene            2226..2630
                     /gene="hptB2"
     CDS             2226..2630
                     /codon_start=1
                     /gene="hptB2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /note="lasso peptide protease"
                     /product="lasso peptide protease"
                     /protein_id="QCY49169.1"
                     /sec_met_domain="PF13471 (E-value: 3.6e-30, bitscore: 92.6,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVPVALPPRRRGARLRRPAPLIAVAVARLLAKAPPRRLRRVMEI
                     ARHRARPATAAQALAAREAVVAVSLRCAGQNCLQRSIATALLCRMRGCWPTWCTGVRTE
                     PFAAHAWVEAEGQLIGEPYPPGYYVPVLSV"
ORIGIN
        1 atgtacgagc aggagctcaa ggaagagttc accgcgcagg actacgttcc cccgatgctg
       61 gccgaggtcg gcgaattcag cgaggacacg ctgggctacg gaaacgcgtg ggacagcaag
      121 aacggcctct tctaagatag gtctgaacag tgattgacac cgggtacgta gtacttccgg
      181 atcactcggc agccgaaaat gccatcgggt cgacggcagc ccgatttccg ctcgtggcgt
      241 cgcacgattc gggaaggccg tggctgttgg ggcggcggcc ggcaggtcag gttgtcgtgg
      301 cgacgaaggg cccggtgcgg gtggcggtga tcggggcttg cccggtcacc gccgcccagt
      361 tgggccggtt cgcctcgtac gtgcgaagcc tctccgatgt gggaaccctg gcgaggaaac
      421 tcccggggtc gagcatcctg gcggtatccg tacacggcca gacgcgtgtc caagggagca
      481 tcgtcggttt gcaccccgtc ttccacgctc gtgtgcgggg cgtggacgtg gccgcagaca
      541 gggccgatct cctggccgcc atggccgacg cgagcatcga cgaggagagc ctcgccgttc
      601 gcgtcgcatg tggccgactg ctgccggccc cgctgagcca cagaccgctg tggggcggcg
      661 tctcggccct ctctccggac cactgcctgg tctgggagaa ggagcgggcg cgcgagatgc
      721 gctggtgggc gcctccggct cccgaacagt ccctcggcga aggcgccggc aaggtgcggg
      781 aggtcatggc ggccgccatg gcgcacaggg caccggagga aggccgactc agcactgacc
      841 tgtcgggggg catggactcc acctctttgt gtttcctggc cgctcgcaag actcccgaca
      901 tactcacgtt ccggaccgct gaggccgacg tcaccaacga cgacgccctg ttcgcctctc
      961 acagcatcag cgcactgccg gccgcggaac acatggtcgc cccccaggag gatctgccgt
     1021 cgatcttcac cgatcccggc tcctgggcga gcaccgagga gccgtacccg ttcatccgca
     1081 cgggggccca gactcgctac gtcgcgcgac tcctggcgga acacggttca cggcgccacc
     1141 tcgcgggaca cggaggggac gaactgttca cctccctcgc tggttacctg cacgaactcc
     1201 tgctccgtcg gccgggcacg gcattcagac acgtgcgcgg atacagcgct ctcaagcggt
     1261 ggcccctggc ggagaccgcg gctgccctcc tgcgcccggg cacggtgcgg tcctggtggc
     1321 aggaccaggc cacacacctc accgccccac cgcccccgct gcgcaggccg gtgctcgggt
     1381 ggggtctgtg gccgcttcgg gcctctccct gggtcaccga gactgccaac gatctcgcac
     1441 ggacggcatt cctgcggacc gctgtcgaga ccccccggta cgccgagaac ctcgggcagc
     1501 accagctgtt gctcatcttg cgcagcagtg ccttcctgca ccgcctcctc agggaaccgt
     1561 tcgcgcggga gtcgatccac ctggacatgc cgttcttcga cgaccggctg gtcgaggcgg
     1621 tgctcgccgt gcggccggag gagcggtcgg atccgtggcg ctacaaaccc ctgctgtccg
     1681 aagcgatgag aggggtcctg cccgacatgg tcctcggccg gtccacaaag ggggagttca
     1741 gcgaggacgt ccgcatcggc atgaagggca atgtgcccgc cctgctcgac gtgttcgccg
     1801 actcccggct cgccgccagg ggcctcatcg acgccgaccg gctgaggtcg agcctcctgg
     1861 ccccccagcg gaacaccacc atcctgcaag acatcgagta cctcctcggc tgcgaatcgt
     1921 ggctgcgtgc ggtggaggcc tcgacaccga ctcactccgg ggagccttgt gtccatccgt
     1981 ctgcgccctg atgtgtccct cgccgaaacc cccgacggca cggtactcct ggaccaggcc
     2041 aaggggcgct actggcagct gaacgccacg ggtgcccgca tactcaggcg tctcttggac
     2101 ggtcacagtc cggaggagat cgcccgggcc atcgcggcgg gttgcggtat cggcctcgac
     2161 cgcgcgctgc gggacgtcga cctggtcacg gaacaactcg accgcgccgg tctggcggtg
     2221 tcccgatgag cgttcccgtc gcacttccgc cacgccgccg aggagcacgg ctacgccgtc
     2281 cggcaccact gatcgccgtc gcggtcgccc ggcttctggc caaagccccg ccacgacggc
     2341 tgcgccgcgt gatggagatc gcacgacacc gtgccaggcc ggcgaccgcc gcccaggccc
     2401 tcgcggcacg tgaggcagtg gtggcggtca gcctgcggtg cgccggccag aactgcctgc
     2461 agcgttccat agcgacggcc ttgctctgcc gcatgcgggg ctgctggccg acctggtgca
     2521 ccggtgtccg caccgaaccg ttcgccgctc acgcctgggt cgaggcggaa ggccagctga
     2581 tcggcgagcc ctacccgccg ggctattacg tgcctgtgct ctccgtgtga
//
