BGC0002075: pyreudione A biosynthetic gene cluster from Pseudomonas fluorescens
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Location: 27,345 - 31,238 nt. (total: 3,894 nt).
This entry is originally from NCBI GenBank NZ_LVEJ01000014.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
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Gene details
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General
Compounds
Genes
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0002075
Short description pyreudione A biosynthetic gene cluster from Pseudomonas fluorescens
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Remarks "The single NRPS is multispecific with regards to amino acid activation (L-proline, L-pipecolic acid) and C starter domain substrate specificity (3-oxo fatty acids of different length and saturation), thereby allowing to form different tetramic acids via Dieckmann-type cyclization. Depending on the amino acid substrate, acyltetramic acids with a pyrrolizidine (A domain substrate: L-proline) or an indolicidine (A domain substrate: L-pipecolic acid) core structure are formed."
Biosynthetic class(es)
  • NRP (Other lipopeptide)
  • Alkaloid (Pyrrolizidine)
Loci NCBI GenBank: NZ_LVEJ01000014.1
Compounds
  • pyreudione A
  • pyreudione B
  • pyreudione C
  • pyreudione D
  • pyreudione E
Species Pseudomonas fluorescens [taxonomy]
References
Chemical products information
pyreudione A
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C15H23N1O3
pyreudione B
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C17H27N1O3
Chemical database entries
NPAtlas
pyreudione C
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Chemical database entries
PubCHEM
NPAtlas
pyreudione D
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C19H29N1O3
Chemical database entries
NPAtlas
pyreudione E
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C16H25N1O3
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • A8O26_RS14270
  • WP_064118616.1
 27345 - 31238 (+) non-ribosomal peptide synthetase
  • Scaffold biosynthesis
Activity assay
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NRP-specific information
Subclass Other lipopeptide
Cyclic? no
Lipid moiety 3-oxo fatty acids, variably length and saturation
NRP-synthases
Gene Modules
A8O26_RS14270
Module 1
A feeding study proved A domain specificity

Specificity: proline / pipecolic acid / 4-hydroxyproline / 4-oxoproline / azetidine-2-carboxylic acid
Evidence for specificity:
  • ATP-PPi exchange assay
  • Mass spectrometry
  • Structure-based inference
Condensation domain type: Starter
Annotation changelog
MIBiG version Submitter Notes
3.0
  • Hidden contributor (ID: 6CADHILFODRMCII55WHSHZ6V, no GDPR consent given).
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Submitted
  • Corrected NRP module activity
  • Removed gene names of 'No gene ID'
  • Added version to NCBI accession
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
  • Changed chemical structures for compounds: pyreudione E, pyreudione B, pyreudione D.
Detailed domain annotation
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