LOCUS       BGC0002075              3894 bp    DNA     linear   CON 08-JUL-2022
DEFINITION  Pseudomonas fluorescens strain HKI0770 contig14, whole genome
            shotgun sequence.
ACCESSION   BGC0002075
VERSION     BGC0002075
KEYWORDS    .
SOURCE      Pseudomonas fluorescens
  ORGANISM  Pseudomonas fluorescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            LVEJ01000014.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 16:51:15
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3894
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            1..3894
                     /locus_tag="A8O26_RS14270"
     protocluster    1..3894
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:3894]"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..3894
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     cand_cluster    1..3894
                     /SMILES="N1C(CCC1)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..3894
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002075"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..3894
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX))"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             1..3894
                     /NRPS_PKS="Domain: Condensation_Starter (6-303). E-value:
                     2.8e-64. Score: 209.2. Matches aSDomain:
                     nrpspksdomains_A8O26_RS14270_Condensation_Starter.1"
                     /NRPS_PKS="Domain: AMP-binding (463-849). E-value: 5.4e-95.
                     Score: 310.2. Matches aSDomain:
                     nrpspksdomains_A8O26_RS14270_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (954-1023). E-value: 2.3e-20. Score:
                     64.5. Matches aSDomain: nrpspksdomains_A8O26_RS14270_PCP.1"
                     /NRPS_PKS="Domain: Thioesterase (1042-1265). E-value:
                     3.3e-30. Score: 98.0. Matches aSDomain:
                     nrpspksdomains_A8O26_RS14270_Thioesterase.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127:condensation domain-containing protein (Score:
                     244.7; E-value: 3.6e-74)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011533552.1"
                     /locus_tag="A8O26_RS14270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_064118616.1"
                     /sec_met_domain="Condensation (E-value: 2.9e-42, bitscore:
                     130.8, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.4e-102, bitscore:
                     329.4, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.6e-16, bitscore:
                     46.2, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRDVHPCPLSTAQQEVCLAISQSHSNLNYHLCDVIELRGELHLPW
                     LEAAIRAQFLQTDTLRATFDIDPTTGTYAQHIQPADALPAKAFESLDVSDQADPAQASN
                     ELLEHLLDQDMDLQHGPLIRYVLIRLAPQHYRMIELASHLVVDGFGHGILFGNITAHYN
                     ALSRGETVEALELAPLSSVFDAQEEYRHSDMHKKDRTYWRQYCLKMPEPTQLVPGDAPL
                     IKLNRLRKVFGGATLLQLRAAASEHQLRLSSILLALCATYLQRMTGQHELALGMPVAAR
                     QLKALRNVPSMVANILPLHLSFTPESTVLSVAANLQRQLRHHLLHQSYRSESMIRDLHA
                     ERGNKPLFNTLLNIVAYDQGPGFAGCDTTIQNVANGPADHLGIDIFDRHDDGRLEIGFN
                     ANADLYSAEALELHYQRLTALFERFANAPQTLAADYNLFLPDEQQRHYALPPHPEKLPV
                     FAEAFANAVHDYAERPALSQGAQTLSYRRLDDDATRLAAHLRERGVRAGDCVVVMFSRS
                     VEWAVAAVALFKLGACYVPVDPDLPEARIEHIFSDADPAVVIVAPGSQLKVEVAADKLL
                     RLSAETLAQLPAVTQPLSAFDAGLPGYLIYTSGSTGKPKGVEVTQRNLVPIARTAINAA
                     QLQPGARVLQFIAAGFDMSVLEIMMTLLAGAELVITDKVSSAPGKALATLVKREGINLL
                     VMTPSLLACHQTEDFPQDTVLMLGGEPCTPALLARFAHCRLLNVYGPTETSFATSINAC
                     YGAGDLSIGPATANTRLYVVDGQQRLLPPGAWGDLFIGGPGVARGYRNRPDLTAKGFVA
                     DLLDSASTMYRAGDRVFFDHAGRIHYLGRQDNQIKLRGLRIELDEIKNVLLGCAGVDDA
                     TVILRELGHGPAIVGYVASKDSSLDGQHLKQALGRHLPQHMVPSVIMCVSEFPLTPNGK
                     LAVDRLPAPALYDATELAPAQNAEEAAMCKLFAEALDCNEVFANQNFFDLGGHSLLGLQ
                     LVSRIKEQFGIALGIADFLAAPTPRQLAQRLQTNSGDSDPFDAVLTLRSEGSRPPLFCI
                     HPGGGIAWPYAGLLPFLPEDQPMYALQSPILRDPTRVIGSLDELAAEYLQRIVDLHPEG
                     PYQLAGWSVGGNLALRIGAMLQARGREVSFLCMFDSYPLQGGPASLKLDDAMIISRMTR
                     AIVGTPRAGLKGLKSAMEEVLGSRQIGDEFLTRLVDDSKLMLELLGRTQYEVFNGDLLF
                     IRATTDILRQDEQQPGLWAPYISGELIQHDVEAPHECLLQRQYLEQFGKAFVEALLKRQ
                     ASPASETQKLSEV"
     aSDomain        19..909
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A8O26_RS14270_Condensation_Start
                     er.1"
                     /domain_subtypes="Condensation_Starter"
                     /evalue="2.80E-64"
                     /label="A8O26_RS14270_Condensation_Starter.1"
                     /locus_tag="A8O26_RS14270"
                     /protein_end="303"
                     /protein_start="6"
                     /score="209.2"
                     /tool="antismash"
                     /translation="CPLSTAQQEVCLAISQSHSNLNYHLCDVIELRGELHLPWLEAAIR
                     AQFLQTDTLRATFDIDPTTGTYAQHIQPADALPAKAFESLDVSDQADPAQASNELLEHL
                     LDQDMDLQHGPLIRYVLIRLAPQHYRMIELASHLVVDGFGHGILFGNITAHYNALSRGE
                     TVEALELAPLSSVFDAQEEYRHSDMHKKDRTYWRQYCLKMPEPTQLVPGDAPLIKLNRL
                     RKVFGGATLLQLRAAASEHQLRLSSILLALCATYLQRMTGQHELALGMPVAARQLKALR
                     NVPSMVANILPLHLSF"
     aSModule        19..3795
                     /complete
                     /domains="nrpspksdomains_A8O26_RS14270_Condensation_Starter
                     .1"
                     /domains="nrpspksdomains_A8O26_RS14270_AMP-binding.1"
                     /domains="nrpspksdomains_A8O26_RS14270_PCP.1"
                     /domains="nrpspksdomains_A8O26_RS14270_Thioesterase.1"
                     /final_module
                     /locus_tags="A8O26_RS14270"
                     /monomer_pairings="Pro -> Pro"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       85..192
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A8O26_RS14270_0001"
                     /evalue="9.50E-05"
                     /label="C2_starter_024-063"
                     /locus_tag="A8O26_RS14270"
                     /protein_end="64"
                     /protein_start="28"
                     /score="15.1"
                     /tool="antismash"
                     /translation="YHLCDVIELRGELHLPWLEAAIRAQFLQTDTLRATF"
     CDS_motif       811..900
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A8O26_RS14270_0002"
                     /evalue="2.90E-12"
                     /label="C5_starter_265-294"
                     /locus_tag="A8O26_RS14270"
                     /protein_end="300"
                     /protein_start="270"
                     /score="38.9"
                     /tool="antismash"
                     /translation="ELALGMPVAARQLKALRNVPSMVANILPLH"
     CDS_motif       949..1062
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A8O26_RS14270_0003"
                     /evalue="3.30E-11"
                     /label="C67_starter_14fromHMM"
                     /locus_tag="A8O26_RS14270"
                     /protein_end="354"
                     /protein_start="316"
                     /score="35.7"
                     /tool="antismash"
                     /translation="QRQLRHHLLHQSYRSESMIRDLHAERGNKPLFNTLLNI"
     aSDomain        1390..2547
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A8O26_RS14270_AMP-binding.1"
                     /evalue="5.40E-95"
                     /label="A8O26_RS14270_AMP-binding.1"
                     /locus_tag="A8O26_RS14270"
                     /protein_end="849"
                     /protein_start="463"
                     /score="310.2"
                     /specificity="substrate consensus: Pro"
                     /tool="antismash"
                     /translation="ANAVHDYAERPALSQGAQTLSYRRLDDDATRLAAHLRERGVRAGD
                     CVVVMFSRSVEWAVAAVALFKLGACYVPVDPDLPEARIEHIFSDADPAVVIVAPGSQLK
                     VEVAADKLLRLSAETLAQLPAVTQPLSAFDAGLPGYLIYTSGSTGKPKGVEVTQRNLVP
                     IARTAINAAQLQPGARVLQFIAAGFDMSVLEIMMTLLAGAELVITDKVSSAPGKALATL
                     VKREGINLLVMTPSLLACHQTEDFPQDTVLMLGGEPCTPALLARFAHCRLLNVYGPTET
                     SFATSINACYGAGDLSIGPATANTRLYVVDGQQRLLPPGAWGDLFIGGPGVARGYRNRP
                     DLTAKGFVADLLDSASTMYRAGDRVFFDHAGRIHYLGRQDNQIKLR"
     CDS_motif       1807..1860
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A8O26_RS14270_0004"
                     /evalue="2.80E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="A8O26_RS14270"
                     /protein_end="620"
                     /protein_start="602"
                     /score="26.0"
                     /tool="antismash"
                     /translation="YLIYTSGSTGKPKGVEVT"
     CDS_motif       2341..2430
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A8O26_RS14270_0005"
                     /evalue="1.00E-14"
                     /label="NRPS-A_a6"
                     /locus_tag="A8O26_RS14270"
                     /protein_end="810"
                     /protein_start="780"
                     /score="46.6"
                     /tool="antismash"
                     /translation="PPGAWGDLFIGGPGVARGYRNRPDLTAKGF"
     CDS_motif       2512..2574
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A8O26_RS14270_0006"
                     /evalue="3.20E-09"
                     /label="NRPS-A_a8"
                     /locus_tag="A8O26_RS14270"
                     /protein_end="858"
                     /protein_start="837"
                     /score="29.0"
                     /tool="antismash"
                     /translation="YLGRQDNQIKLRGLRIELDEI"
     aSDomain        2863..3069
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A8O26_RS14270_PCP.1"
                     /evalue="2.30E-20"
                     /label="A8O26_RS14270_PCP.1"
                     /locus_tag="A8O26_RS14270"
                     /protein_end="1023"
                     /protein_start="954"
                     /score="64.5"
                     /tool="antismash"
                     /translation="EAAMCKLFAEALDCNEVFANQNFFDLGGHSLLGLQLVSRIKEQFG
                     IALGIADFLAAPTPRQLAQRLQTN"
     aSDomain        3127..3795
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A8O26_RS14270_Thioesterase.1"
                     /evalue="3.30E-30"
                     /label="A8O26_RS14270_Thioesterase.1"
                     /locus_tag="A8O26_RS14270"
                     /protein_end="1265"
                     /protein_start="1042"
                     /score="98.0"
                     /tool="antismash"
                     /translation="PPLFCIHPGGGIAWPYAGLLPFLPEDQPMYALQSPILRDPTRVIG
                     SLDELAAEYLQRIVDLHPEGPYQLAGWSVGGNLALRIGAMLQARGREVSFLCMFDSYPL
                     QGGPASLKLDDAMIISRMTRAIVGTPRAGLKGLKSAMEEVLGSRQIGDEFLTRLVDDSK
                     LMLELLGRTQYEVFNGDLLFIRATTDILRQDEQQPGLWAPYISGELIQHDVEAPHECLL
                     Q"
     CDS_motif       3130..3159
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A8O26_RS14270_0007"
                     /evalue="1.00E-03"
                     /label="NRPS-beforete1"
                     /locus_tag="A8O26_RS14270"
                     /protein_end="1053"
                     /protein_start="1043"
                     /score="12.0"
                     /tool="antismash"
                     /translation="PLFCIHPGGG"
     CDS_motif       3310..3384
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A8O26_RS14270_0008"
                     /evalue="8.40E-10"
                     /label="NRPS-te1"
                     /locus_tag="A8O26_RS14270"
                     /protein_end="1128"
                     /protein_start="1103"
                     /score="31.1"
                     /tool="antismash"
                     /translation="HPEGPYQLAGWSVGGNLALRIGAML"
ORIGIN
        1 atgcgtgacg tacacccgtg cccgctttcc acggcgcaac aggaagtttg cctggcgatc
       61 agccagagcc acagcaatct caactaccac ttgtgcgacg tcatcgaact gcgcggcgag
      121 ctgcatctgc cgtggctcga agccgcgatc cgcgcgcaat tcctgcagac cgacaccctg
      181 cgcgcgacct tcgacatcga cccgacaacc ggcacctacg cgcagcacat ccagcctgcc
      241 gatgccctgc ccgccaaggc cttcgaaagt ctcgacgtca gtgatcaagc cgatcctgca
      301 caggcctcca acgaactgct cgaacacttg ctggatcagg acatggatct gcaacacggg
      361 ccgttgatcc gttacgtgct gatccgcctc gcaccgcagc attaccgcat gatcgaactg
      421 gcctcgcacc tggtggtgga cggtttcggc catggcattc tgttcggcaa tatcaccgcc
      481 cattacaacg ccctcagccg tggcgaaacg gtcgaagcgc tggagctggc gccgctgtcg
      541 agcgtgttcg acgcgcaaga ggaatatcgc cacagcgaca tgcacaaaaa ggaccgcacc
      601 tactggcgtc agtactgcct gaagatgccc gagccgacgc aactggtgcc tggcgatgcg
      661 ccgctgatca aactcaatcg tctgcgcaag gtgttcggcg gtgcaaccct gctgcaactg
      721 cgcgcggcgg ccagcgaaca tcaactgcgc ctgtcgagca ttctgctggc gctgtgcgcg
      781 acctatctgc aacgcatgac cgggcaacac gaactggccc tgggcatgcc ggtcgcagcg
      841 cgtcagctca aggcgttgcg caatgtgccg tcaatggtcg ccaacatcct gccgctgcac
      901 ttgtcgttca cgcctgaaag caccgtgctg agcgtggcgg caaacctgca gcgacagttg
      961 cgtcatcacc tgctgcacca aagctatcgc agcgaaagca tgatccgcga cctgcatgcc
     1021 gagcgcggta acaagccgct gttcaacaca ctgctgaaca tcgttgccta cgatcagggc
     1081 ccgggatttg ccgggtgtga caccaccatc cagaacgtcg ccaatggccc ggccgatcac
     1141 ttgggcattg atattttcga ccgccacgac gatggtcggc tggagatcgg tttcaatgcc
     1201 aacgccgatc tctactccgc cgaggcgctg gaactgcatt atcagcgcct gactgcgctg
     1261 ttcgaacgct tcgccaacgc gccgcaaacc ctggctgccg actacaacct gttcctgccc
     1321 gatgagcagc agcgccatta cgcactgccg ccgcatccag aaaagctgcc ggtgttcgcc
     1381 gaagcctttg ccaacgccgt gcacgattac gccgagcgtc cggcactgtc gcaaggcgca
     1441 cagaccttga gctaccgccg cctcgacgat gacgccacgc gcctcgctgc acacttgcgt
     1501 gaacgcggcg tgcgcgccgg tgactgcgtg gtggtgatgt tctcgcgcag tgtcgaatgg
     1561 gcggtggccg cggtggccct gttcaaactc ggcgcctgct atgtgccggt ggatccggat
     1621 ctgcccgagg cgcgcatcga acacatcttc agcgatgccg acccggccgt ggtgatcgtt
     1681 gccccgggca gtcagctcaa ggttgaggtc gccgccgaca aactgctgcg actgagcgct
     1741 gaaaccctgg cgcaattacc ggcggtgact cagccgttgt cggcatttga tgccggcctg
     1801 ccgggctatc tgatctacac ctccggttcc accggcaagc ccaaaggcgt cgaagtcacc
     1861 cagcgcaatc tggtgccgat tgcccgcacc gcgatcaatg ccgcgcaatt gcagccaggc
     1921 gcgcgagtcc tgcaattcat cgccgccggg ttcgacatgt cggtgctgga aatcatgatg
     1981 accttgctcg ccggagccga actggtgatc accgacaaag tcagcagtgc tcccggcaaa
     2041 gccttggcta cattggtcaa acgcgaaggc atcaacctgt tggtgatgac cccgagtctg
     2101 ctcgcctgcc atcagaccga agacttcccg caggacaccg tgctcatgct cggcggtgaa
     2161 ccgtgcacac cggcattgct cgcacgcttc gcgcactgcc gcttgctcaa cgtttacggg
     2221 ccgacagaaa cctcgttcgc cacctcgatc aatgcctgtt acggcgcggg cgatctgtcg
     2281 atcgggccgg ccacggccaa cacccggctg tacgtggtcg acggccagca gcgcttgctg
     2341 ccacccggcg cgtgggggga tctgttcatc ggcggtccgg gcgtggcgcg tggttatcgc
     2401 aaccgtcccg acctgactgc caaaggcttt gttgccgatc tgctcgacag cgccagcacc
     2461 atgtaccgcg ccggtgaccg ggtgttcttc gaccatgccg ggcgcattca ttaccttggc
     2521 cgtcaggaca accagatcaa attgcgcggc ctgcgcatcg agctcgacga gatcaaaaac
     2581 gtcctgctcg gctgcgccgg ggtcgacgac gccacggtga tactgcgtga gcttggccac
     2641 ggtccggcca ttgtcggcta tgtcgcgagc aaggactcca gccttgatgg tcaacatctg
     2701 aaacaggcgc tgggccgtca cctgcctcag cacatggtgc cgagcgtgat catgtgcgtc
     2761 agcgaattcc cgctgacacc caacggcaaa ctcgcggtgg atcgcctgcc ggcgccggca
     2821 ctctacgacg ccaccgaact ggcgcccgcg caaaacgcgg aagaagcggc gatgtgcaag
     2881 ctgttcgccg aagcgctgga ctgcaacgaa gtgttcgcca accagaattt cttcgacctt
     2941 ggcggccatt cgttgctggg gttgcaactg gtcagcagga tcaaggagca attcggtatc
     3001 gccttgggca tcgccgattt cctcgccgcg ccaacgccgc gtcaattggc ccagcgcctg
     3061 caaaccaaca gtggcgacag cgatccgttc gacgccgtgc tgacgctgcg cagcgaaggc
     3121 tcgcggccgc cactgttctg catccatccg ggcggcggga ttgcctggcc ctacgccggg
     3181 ctgctgccgt ttctgccgga agaccagccg atgtacgcgt tgcaatcgcc gatcctgcgt
     3241 gatccgacgc gggtcatcgg atcgctggat gagctggctg ccgaatacct gcaacgcatc
     3301 gttgatctgc accccgaggg tccgtatcaa ctggccggtt ggtcggtggg tggcaacctc
     3361 gcgctgcgta tcggcgcgat gctgcaggcg cgcgggcgtg aggtgagttt cctgtgcatg
     3421 ttcgacagct acccgctgca aggcggtccg gcctcgttga aactcgacga cgcgatgatc
     3481 atcagccgca tgacccgcgc catcgttggc acaccacgtg ccgggctcaa ggggttgaaa
     3541 agtgccatgg aagaagtgct cggcagccgg cagatcggcg atgagttcct gactcgtctg
     3601 gtggacgatt ctaagttgat gctggagttg ctcggacgca cgcagtacga ggtgttcaac
     3661 ggtgacttgc tgttcatccg cgccaccacc gacatcctgc gtcaggacga gcagcagccg
     3721 ggattgtggg cgccgtatat cagcggtgag ttgatccagc acgacgttga agcaccgcat
     3781 gaatgcctgc tgcaacgaca atatctggag cagttcggga aggcgtttgt cgaggcgttg
     3841 ctcaagcgtc aggccagtcc agcctctgaa actcagaagc tgagcgaagt ctaa
//
