BGC0002078 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002078 (uncultured bacterium)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,butyrolactone	1	81,534	omnipeptin	NRPS:Type I	100%

BGC0002078 - Region 1 - NRPS,butyrolactone

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Location: 1 - 81,534 nt. (total: 81,534 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
butyrolactone: AfsA

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 81,535
Label		BGC0002078

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002078	0.93	NRP	omnipeptin	uncultured bacterium
BGC0002079	0.55	NRP	omnipeptin	uncultured bacterium
BGC0000849	0.48	Other (Butyrolactone)	SCB1, SCB2, SCB3	Streptomyces coelicolor A3(2)
BGC0001133	0.45	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0000850	0.41	Other	γ-butyrolactone	Streptomyces acidiscabies
BGC0001629	0.41	NRP	jessenipeptin	Pseudomonas sp. QS1027
BGC0002071	0.37	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002017	0.36	NRP	potensibactin	Nocardiopsis potens DSM 45234
BGC0000417	0.35	NRP	rhodochelin	Rhodococcus jostii RHA1
BGC0000848	0.35	Other	A-factor	Streptomyces griseus subsp. griseus NBRC 13350

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002078	1.00	NRP	omnipeptin	uncultured bacterium
BGC0002079	0.81	NRP	omnipeptin	uncultured bacterium
BGC0000849	0.77	Other (Butyrolactone)	SCB1, SCB2, SCB3	Streptomyces coelicolor A3(2)
BGC0001133	0.75	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0000850	0.72	Other	γ-butyrolactone	Streptomyces acidiscabies
BGC0001629	0.72	NRP	jessenipeptin	Pseudomonas sp. QS1027
BGC0000331	0.70	NRP	cyanopeptolin	Planktothrix agardhii NIVA-CYA 116
BGC0002071	0.70	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002017	0.69	NRP	potensibactin	Nocardiopsis potens DSM 45234
BGC0000417	0.68	NRP	rhodochelin	Rhodococcus jostii RHA1

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 65518:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Asp - bOH-Tyr - Ser - D-Glu) + (Thr - Ser - X - X - X - D-Val) + (Phe - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

QEO74979.1

CAL_domain (32..457): pk

minowa: NH2

Prediction, score:

NH2: 133.4
fatty_acid: 19.6
Acetyl-CoA: 12.5
AHBA: 3.9
shikimic_acid: 0.0

QEO74981.1

Search NORINE for peptide: strict or relaxed

AMP-binding (451..822): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGAVNK
Nearest Stachelhaus code(s):: Asp DLTKVGAVNK (100% 8Å match); Asp DLTKVGAVNK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1478..1846): bOH-Tyr

nrpys: bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DASTIAAVDK
Nearest Stachelhaus code(s):: bOH-Tyr DASTIAAVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2499..2896): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHISLVDK
Nearest Stachelhaus code(s):: Ser DVWHISLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3533..3888): Glu

nrpys: Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLKVGAVNK
Nearest Stachelhaus code(s):: Glu DVLKVGAVNK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

QEO74982.1

Search NORINE for peptide: strict or relaxed

AMP-binding (450..852): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNVGMVHK
Nearest Stachelhaus code(s):: Thr DFWNVGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1495..1874): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHISLVDK
Nearest Stachelhaus code(s):: Ser DVWHISLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2433..2805): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMPQFGLVWK
Nearest Stachelhaus code(s):: D-Ala DMPQLGMVWK (68% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (3455..3834): X

nrpys: bMe-Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGEVGK
Nearest Stachelhaus code(s):: bMe-Asn DLTKVGEVGK (100% 8Å match); Asn DLTKVGEVGK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4490..4882): X

nrpys: 6-clTrp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAAMCGGVAK
Nearest Stachelhaus code(s):: 6-clTrp DAAMCGGVAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5550..5915): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAYWWGGTFK
Nearest Stachelhaus code(s):: Val DAYWWGGTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

QEO74983.1

Search NORINE for peptide: strict or relaxed

AMP-binding (448..816): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAFVLAGVAK
Nearest Stachelhaus code(s):: Phe DAFVLAGVAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1465..1861): X

nrpys: 3-oh-4-mePro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DGQYVSQVMK
Nearest Stachelhaus code(s):: 3-oh-4-mePro DGQYVSQVMK (100% 8Å match)
Stachelhaus code match:: 100% (strong)