LOCUS       BGC0002091             17973 bp    DNA     linear   BCT 01-JUN-2009
DEFINITION  Yersinia ruckeri ATCC 29473 contig00403, whole genome shotgun
            sequence.
ACCESSION   BGC0002091
VERSION     BGC0002091
KEYWORDS    .
SOURCE      Yersinia ruckeri ATCC 29473
  ORGANISM  Yersinia ruckeri ATCC 29473
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Yersinia.
COMMENT     Genome coverage: 37.5X.
            ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-08 06:43:25
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..17973
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[6188:9560]"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      6189..9560
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     cand_cluster    1..17973
                     /SMILES="NC(CS)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..17973
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002091"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..17973
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            2..571
                     /locus_tag="yruck0001_11670"
     CDS             2..571
                     /codon_start=1
                     /locus_tag="yruck0001_11670"
                     /note="similar to hypothetical protein PRK11469; similar to
                     UPF0059 membrane protein plu2701 of Proteobacteria UniRef
                     RepID=Y2701_PHOLL"
                     /product="hypothetical protein"
                     /protein_id="EEP98509.1"
                     /transl_table=11
                     /translation="MNLSATLILAFAMSMDAFAASIGKGATLYKPRFREAIRTGLIFGV
                     IEAITPLIGWGIGLFASQYIMEWDHWIAFALLFILGCRMIIEGCKGPTEDEPQVRRHSF
                     WVLVMTAIATSLDAMAIGVGLAFLQVNILHTAMAIGMATMIMATLGMLIGRYIGPLLGK
                     RAEIIGGIILIGIGFNILYEHLYKLA"
     gene            complement(658..1203)
                     /locus_tag="yruck0001_11680"
     CDS             complement(658..1203)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Flavoprotein"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="yruck0001_11680"
                     /note="similar to bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate synthase PRK05579;
                     similar to Lantibiotic biosynthesis protein, flavoprotein
                     of Phaeobacter gallaeciensis UniRef RepID=A9EZF7_9RHOB"
                     /product="Lantibiotic biosynthesis protein, flavoprotein"
                     /protein_id="EEP98510.1"
                     /sec_met_domain="Flavoprotein (E-value: 3e-24, bitscore:
                     75.6, seeds: 143, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKKRILVGATGSVDITLLPGYLRAIKASIDCSLTLLMTPQATGF
                     IPAETMRLWADRVIAGERPTDWPTDKPSKIVADHDIMLVLPATANTLADAAQGAAPNRL
                     TTVILASDFPVLFFPVMGAVMWDKPAVQRNIRQLREDGYEVLEPEWHENYDTALGKKVS
                     HPSLPSASTLVSLLIERL"
     gene            complement(1265..1696)
                     /locus_tag="yruck0001_11690"
     CDS             complement(1265..1696)
                     /codon_start=1
                     /locus_tag="yruck0001_11690"
                     /note="similar to Globin of Actinomycetales UniRef
                     RepID=A8LDV0_FRASN"
                     /product="Globin"
                     /protein_id="EEP98511.1"
                     /transl_table=11
                     /translation="MGGREVLLKLMTVFYAKIEKDELLAPMFVHMHADHPEHVAIWLEE
                     VLGGEANYTAHRGGFKGMVAKHRGRAIQPEQRKRWVDLMMECADEVNLPADPEFRSAFA
                     GYIEFGSRRAQANSQPKAERSKRETVKLWGWGEAPPGTP"
     gene            complement(1777..2649)
                     /locus_tag="yruck0001_11700"
     CDS             complement(1777..2649)
                     /codon_start=1
                     /locus_tag="yruck0001_11700"
                     /note="similar to hypothetical protein PRK13688; similar to
                     Acetyltransferase of Streptomyces clavuligerus ATCC 27064
                     UniRef RepID=B5GVJ0_STRCL"
                     /product="Acetyltransferase"
                     /protein_id="EEP98512.1"
                     /transl_table=11
                     /translation="MMKNVVKSTDKAFSVHLATPAEWQSAVEMARNEHWDLGHGDDRIF
                     LNVDKNGFFIGRTEGRIVASISIVNFDPGYAHLGHYIVTPEYRGKGLGLELWTAAIEHA
                     GERCIGLDGMPMQEENYKKWGFRTHYHTYRIQGRASGIAIKADNISEVSSHHLSAIADF
                     DTSFSGCNRASLLSSWFTADNRQGFVVTEGASVKGVIALRPSDEGYRIGPFYAPDENHA
                     IALLQTALGALPKNALVTLDVPEQAGATLDVLKVAGFSPLFHTCRMYRGTPPVSYQKGN
                     NAVASLELG"
     gene            complement(2674..3804)
                     /locus_tag="yruck0001_11710"
     CDS             complement(2674..3804)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006:acyl-CoA dehydrogenase (Score: 327.5; E-value:
                     1.8e-99)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="yruck0001_11710"
                     /note="similar to Acyl-CoA dehydrogenase fadE20 of Bacteria
                     UniRef RepID=A2VL62_MYCTU"
                     /product="Acyl-CoA dehydrogenase fadE20"
                     /protein_id="EEP98513.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 1.1e-28, bitscore:
                     90.4, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 2.3e-25, bitscore:
                     78.9, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 1.4e-37, bitscore:
                     119.4, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDNKELSAFRASCRTFLEHEAKPYLSVWEKEGLVSRDFWRHAGEA
                     GILGIGVSGRWGGQNQADYQYASVLTEELIRAEMTAPVVIAHNDVIASYIDAHGTDEQR
                     DRWLPGLCKGELIAAIAVTEPNGGSDSADLETTAVRQDQHYVVNGQKSFITNGINADLI
                     LTAVRTSKAERGRGISLLVIERDTAGFTRGAPLKKLGWHASDTANLHFSNCLVPCNNLI
                     GKENIGNFYFMGGMPRERLSISTVAVATAELLLERTLEWVKKRHAFGQPVGSFQHNRFR
                     LAELDTNVRIARIYLNDAIDKFNRRELNVADAARIKLWTTELQIQVADQCMQLHGGAGY
                     LSDSFIGKTWVNSRVQTIYGGTSEVLKEFISKSMGL"
     gene            complement(3821..4552)
                     /locus_tag="yruck0001_11720"
     CDS             complement(3821..4552)
                     /NRPS_PKS="Domain: Thioesterase (1-221). E-value: 8.6e-28.
                     Score: 90.1. Matches aSDomain:
                     nrpspksdomains_yruck0001_11720_Thioesterase.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1004:thioesterase (Score: 145; E-value: 3.4e-44)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="yruck0001_11720"
                     /note="similar to Putative uncharacterized protein of
                     Streptomyces clavuligerus ATCC 27064 UniRef
                     RepID=B5GVJ3_STRCL"
                     /product="hypothetical protein"
                     /protein_id="EEP98514.1"
                     /transl_table=11
                     /translation="MVYAFPHAGAGTGVYRHWLSRFNDESKLVFSPVGIPGRDRLSREK
                     VIDDLAVLASRISADIYADFTQRKKAGITEFATFGHSFGGVLSVIVSHLLADKYAMNPV
                     FSVVSGSIAPTAQAEDDRYLWSDEKILEKMRADNGTPESILREPAIARRLVTALRTDYI
                     LRQQFLAYRDMRVEQPLLLISADKDEHVTAQMQLAWLNHTRSRVSITEIQGGHFAVYDN
                     FNTVRTLLSQSSVEHNVSIPA"
     aSDomain        complement(3890..4549)
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_yruck0001_11720_Thioesterase.1"
                     /evalue="8.60E-28"
                     /label="yruck0001_11720_Thioesterase.1"
                     /locus_tag="yruck0001_11720"
                     /protein_end="221"
                     /protein_start="1"
                     /score="90.1"
                     /tool="antismash"
                     /translation="VYAFPHAGAGTGVYRHWLSRFNDESKLVFSPVGIPGRDRLSREKV
                     IDDLAVLASRISADIYADFTQRKKAGITEFATFGHSFGGVLSVIVSHLLADKYAMNPVF
                     SVVSGSIAPTAQAEDDRYLWSDEKILEKMRADNGTPESILREPAIARRLVTALRTDYIL
                     RQQFLAYRDMRVEQPLLLISADKDEHVTAQMQLAWLNHTRSRVSITEIQGGHFAVYD"
     gene            complement(4573..6180)
                     /locus_tag="yruck0001_11730"
     CDS             complement(4573..6180)
                     /codon_start=1
                     /locus_tag="yruck0001_11730"
                     /note="similar to choline dehydrogenase PRK02106; similar
                     to Glucose-methanol-choline oxidoreductase of Streptomyces
                     clavuligerus ATCC 27064 UniRef RepID=B5GVJ4_STRCL"
                     /product="Glucose-methanol-choline oxidoreductase"
                     /protein_id="EEP98515.1"
                     /transl_table=11
                     /translation="MGLSHYDYIVVGGGTAGCVMASRLSENSAKSVLLIEAGLDSNADD
                     HSSPLNDASRLVLENYNWEYEANVRGAERLSRFLQPDAESHAPRDRRKPFGYRLGKVLG
                     GSSAINGAVALRAFPRDFACWVDMGCPTWSWQDVLPWYRKLENDRDIADETLHGHAGPL
                     LLRRPTAEETLPLETGFASACVSHGIPWVEDFNTGEDAAVGAVPANVLQGAERFDFCRA
                     YLTPARMRSNLQIMTEMTVERVLFANGAATGVEVHRNGKYQTLSASRVVLCAGAIGSAA
                     LLQRSGVGDSELLHKLDIPVVADIPAVGNNLSDHASVVMWALPRASNSKVKTPWRQTAA
                     RMSSGYDEQLDVQLGLMNNVASDTVPGFHNRTEYPFLVGASVMLMRPKAKGRVFIRSRD
                     SDKLPAIDLPLSQNEEDVQRLVGGVRQMWQLINHPEVSVCLEGVQFWRESMMGNDEVMR
                     NAIINLVSPGWHASGTIRMGTSADPESAADKKGNIHGLRNITVADASLFPCIPSMPTNL
                     TTAMVAERIASFLMEENV"
     gene            complement(6189..9560)
                     /locus_tag="yruck0001_11740"
     CDS             complement(6189..9560)
                     /NRPS_PKS="Domain: Heterocyclization (62-359). E-value:
                     3.6e-72. Score: 235.1. Matches aSDomain:
                     nrpspksdomains_yruck0001_11740_Heterocyclization.1"
                     /NRPS_PKS="Domain: AMP-binding (532-926). E-value: 1.5e-95.
                     Score: 312.0. Matches aSDomain:
                     nrpspksdomains_yruck0001_11740_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (1040-1112). E-value: 8.3e-14.
                     Score: 43.4. Matches aSDomain:
                     nrpspksdomains_yruck0001_11740_PCP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127:condensation domain-containing protein (Score:
                     100.9; E-value: 1.6e-30)"
                     /gene_kind="biosynthetic"
                     /locus_tag="yruck0001_11740"
                     /note="similar to Peptide synthetase of Streptomyces
                     clavuligerus ATCC 27064 UniRef RepID=B5GVJ5_STRCL"
                     /product="Peptide synthetase"
                     /protein_id="EEP98516.1"
                     /sec_met_domain="Condensation (E-value: 7.4e-33, bitscore:
                     104.0, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.2e-102, bitscore:
                     333.7, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.5e-10, bitscore:
                     31.7, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKMMREATDKLGLTLSLDNDVPIVLQYDSNSDNVALNLTALMCGI
                     LPAGEINYSDNRREENDSEPFSLTDIQYAYLIGRNAAIPLGGVPASHYTEIEVYNPDFD
                     RLNDALGRVIACHPVMRSALTDSGRQRFLPAMGDDVIPLIDCSGQDPQSVHDQVGKTRI
                     EMIEAMRTQGNVRPFDIRATQIGNRNIRLHLCFDLMFFDLHSIKIIMRDWWEFYENPEM
                     QKTESRLNFSDYISLEKELQKSQQGERDKAYWINKIKNIPGAPELPLHQSPELIRHPVI
                     KTLKKEIPFETINKLRHAAAQNGITLEVLFLGVYTEVLRQWSRRQDFTLTLTQNGRRAY
                     SEDITHITGNFLQSCLLCVNSPSSDSFAERLVSLQTELLLNRWHSSFNGIQVLREMTRL
                     NSDGRAFSLPVVFSNTLNAELEDIVSDDLWRNAARIVHTATRTPGVWLENQLMRINGEL
                     VMNWNYVDGLFPDGMAEAMFEAADKLLCACADNPSVWSQRHSMVTLPKADEKARLDANA
                     TQYHLPPTLLHESLLDAARRFPDKTAVLQGTREVSYRELVTAAHDIATQLHAQVDINPG
                     DIIAVSLHQSPEMLAAILGVLIAGAAYVSVDPQLPEQRQHRLIERCAAKAVLTFDGNVA
                     VQGMVIVVTTIVSGTLRPLPPTPRKQSPDDLAYVIFTSGSTGEPKGVMISHTNAVNTVA
                     DINRRFSVNSQDRVYSIAPAGFDLSVYDYFGVLGAGGSILFAAENEPADPGLWAEQIVK
                     QGVTLWNTVPAPVKALFERAGEQLRDSSLRLVLMSGDWIPVDLPDQIRHVSEKIDVISL
                     GGATEGSIWSIVYPVQTVDTTWKSIPYGKPLANQRFHVLNEWLEPSPKWVTGEIFIAGD
                     GVAQGYLGDDEKTQARFFQHPRTGERLYRTGDLGRYINEGLIEILGREDNQVKINGYRI
                     ELGEIEAALLEHPSASHVVINAVTHPKSGQKQLAAYIVATHSAGYDKNRLENELRETAM
                     ATLPSYMVPMWFVLLDEMPLTPNGKIDRNALPAVWADSVAEDSRNVPADETETRLYSIW
                     AAQLQHSDFGVTDGFFDIGGDSLHAVSLLSAVRQSFRVSPSSEQDMIESLFMNASVRDF
                     SKILAEVTLDPEEALS"
     aSDomain        complement(6225..6440)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_yruck0001_11740_PCP.1"
                     /evalue="8.30E-14"
                     /label="yruck0001_11740_PCP.1"
                     /locus_tag="yruck0001_11740"
                     /protein_end="1112"
                     /protein_start="1040"
                     /score="43.4"
                     /tool="antismash"
                     /translation="ETRLYSIWAAQLQHSDFGVTDGFFDIGGDSLHAVSLLSAVRQSFR
                     VSPSSEQDMIESLFMNASVRDFSKILA"
     aSModule        6225..9374
                     /complete
                     /domains="nrpspksdomains_yruck0001_11740_Heterocyclization.
                     1"
                     /domains="nrpspksdomains_yruck0001_11740_AMP-binding.1"
                     /domains="nrpspksdomains_yruck0001_11740_PCP.1"
                     /locus_tags="yruck0001_11740"
                     /monomer_pairings="cys -> cys"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(6753..6815)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_yruck0001_11740_0005"
                     /evalue="2.80E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="yruck0001_11740"
                     /protein_end="936"
                     /protein_start="915"
                     /score="35.4"
                     /tool="antismash"
                     /translation="LGREDNQVKINGYRIELGEIE"
     aSDomain        complement(6783..7964)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_yruck0001_11740_AMP-binding.1"
                     /evalue="1.50E-95"
                     /label="yruck0001_11740_AMP-binding.1"
                     /locus_tag="yruck0001_11740"
                     /protein_end="926"
                     /protein_start="532"
                     /score="312.0"
                     /specificity="consensus: cys"
                     /tool="antismash"
                     /translation="DAARRFPDKTAVLQGTREVSYRELVTAAHDIATQLHAQVDINPGD
                     IIAVSLHQSPEMLAAILGVLIAGAAYVSVDPQLPEQRQHRLIERCAAKAVLTFDGNVAV
                     QGMVIVVTTIVSGTLRPLPPTPRKQSPDDLAYVIFTSGSTGEPKGVMISHTNAVNTVAD
                     INRRFSVNSQDRVYSIAPAGFDLSVYDYFGVLGAGGSILFAAENEPADPGLWAEQIVKQ
                     GVTLWNTVPAPVKALFERAGEQLRDSSLRLVLMSGDWIPVDLPDQIRHVSEKIDVISLG
                     GATEGSIWSIVYPVQTVDTTWKSIPYGKPLANQRFHVLNEWLEPSPKWVTGEIFIAGDG
                     VAQGYLGDDEKTQARFFQHPRTGERLYRTGDLGRYINEGLIEILGREDNQVKIN"
     CDS_motif       complement(6897..6986)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_yruck0001_11740_0004"
                     /evalue="2.10E-13"
                     /label="NRPS-A_a6"
                     /locus_tag="yruck0001_11740"
                     /protein_end="888"
                     /protein_start="858"
                     /score="42.4"
                     /tool="antismash"
                     /translation="PKWVTGEIFIAGDGVAQGYLGDDEKTQARF"
     CDS_motif       complement(7503..7562)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_yruck0001_11740_0003"
                     /evalue="2.00E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="yruck0001_11740"
                     /protein_end="686"
                     /protein_start="666"
                     /score="35.5"
                     /tool="antismash"
                     /translation="AYVIFTSGSTGEPKGVMISH"
     aSDomain        complement(8484..9374)
                     /aSDomain="Heterocyclization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_yruck0001_11740_Heterocyclizatio
                     n.1"
                     /evalue="3.60E-72"
                     /label="yruck0001_11740_Heterocyclization.1"
                     /locus_tag="yruck0001_11740"
                     /protein_end="359"
                     /protein_start="62"
                     /score="235.1"
                     /tool="antismash"
                     /translation="SEPFSLTDIQYAYLIGRNAAIPLGGVPASHYTEIEVYNPDFDRLN
                     DALGRVIACHPVMRSALTDSGRQRFLPAMGDDVIPLIDCSGQDPQSVHDQVGKTRIEMI
                     EAMRTQGNVRPFDIRATQIGNRNIRLHLCFDLMFFDLHSIKIIMRDWWEFYENPEMQKT
                     ESRLNFSDYISLEKELQKSQQGERDKAYWINKIKNIPGAPELPLHQSPELIRHPVIKTL
                     KKEIPFETINKLRHAAAQNGITLEVLFLGVYTEVLRQWSRRQDFTLTLTQNGRRAYSED
                     ITHITGNFLQSCLLCV"
     CDS_motif       complement(8592..8651)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_yruck0001_11740_0002"
                     /evalue="4.10E-05"
                     /label="Cy4"
                     /locus_tag="yruck0001_11740"
                     /protein_end="323"
                     /protein_start="303"
                     /score="16.5"
                     /tool="antismash"
                     /translation="GITLEVLFLGVYTEVLRQWS"
     CDS_motif       complement(8946..8990)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_yruck0001_11740_0001"
                     /evalue="4.60E-03"
                     /label="Cy3"
                     /locus_tag="yruck0001_11740"
                     /protein_end="205"
                     /protein_start="190"
                     /score="10.4"
                     /tool="antismash"
                     /translation="RLHLCFDLMFFDLHS"
     gene            10393..11754
                     /locus_tag="yruck0001_11750"
     CDS             10393..11754
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005:Drug
                     resistance transporter, EmrB/QacA (Score: 291.6; E-value:
                     1.3e-88)"
                     /gene_kind="transport"
                     /locus_tag="yruck0001_11750"
                     /note="similar to putative sialic acid transporter
                     PRK12307; similar to Transporter, major facilitator family
                     of Octadecabacter antarcticus 307 UniRef
                     RepID=B5J414_9RHOB"
                     /product="Transporter, major facilitator family"
                     /protein_id="EEP98517.1"
                     /transl_table=11
                     /translation="MNNVRLLFSTLAVIQATLIFTIVMIGVPLPAIGTEFGLVQAELVL
                     VSAGYGLPFSGLLLLGGRLTDRYSGRKMFTLGVILFGTASLYGMFVPDYWTLVVVRFIQ
                     GTGAAMTAPALMALLRQLYPDSADFGRAMATWGGVSVLGGAAGFLSSGIVTHWLSWRWM
                     FIIPVVVAFVGLVIVRKVMVATEEKTSAQPGLDLGGALLASSGISLFSFGLIFSGEYPW
                     SAPIVWGSVMVGLLLFVAFLSVEKRVSFPLLPPRFIHNPARAIGLAGILLAAAGMGLIT
                     FLLSLYLQQFQGWPPLATSMAFIPYTLGLLVMNRAAGTLIARYHALTVTIVGLLISAVG
                     LFLLAMINPETDYMTGLLPGLFILPAGASLVFSGSAVLATANIPLHQAGLAGGVMNTAM
                     ELGPTMGLAALMAVAATQVDVIDGYGLAFGTAGCIYVVAAMAAILCYRNIKSPC"
     gene            complement(11811..12359)
                     /locus_tag="yruck0001_11760"
     CDS             complement(11811..12359)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1163:rhodanese domain-containing protein (Score: 89.8;
                     E-value: 1.6e-27)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="yruck0001_11760"
                     /note="similar to thiosulfate:cyanide sulfurtransferase
                     PRK10287; similar to Transcriptional regulator, ArsR
                     family/rhodanese-like domain protein of Gammaproteobacteria
                     UniRef RepID=Q4K6P9_PSEF5"
                     /product="Transcriptional regulator, ArsR
                     family/rhodanese-like domain protein"
                     /protein_id="EEP98518.1"
                     /transl_table=11
                     /translation="MERLAELANISIANASQHLQHLKRVGFVQARRDGKNVLYSLGRGP
                     VQNLLTAIRQYVEYRHNEMSNFVSETLNQRERFESVSREELLNRLNEGDVTLLDVRPYE
                     EFSHGHLPGAINIPVEQLEGRLSELPLAQDIVAYCRGPYCVLSVNAMKVLREHGYNVRC
                     LEDGFPEWKAAGLEVQNHR"
     gene            12618..13124
                     /locus_tag="yruck0001_11770"
     CDS             12618..13124
                     /codon_start=1
                     /locus_tag="yruck0001_11770"
                     /note="similar to Putative uncharacterized protein of
                     Proteobacteria UniRef RepID=Q394U6_BURS3"
                     /product="hypothetical protein"
                     /protein_id="EEP98519.1"
                     /transl_table=11
                     /translation="MKKEAEMDITLQAVAIGIGATLIMDIWAILQKRLLNIPSLDYRLV
                     GRWLGHMSRGQFVHHTILQANAVRGEVLVGWLAHYAIGIIFSFLLITATGPGWLVEPTF
                     LPALIVGIISIVAPFFIMQPGFGFGIAASRLPQPNVARRRSLIAHTSFGVGLYISAQVL
                     RFMLN"
     gene            13375..13626
                     /locus_tag="yruck0001_11780"
     CDS             13375..13626
                     /codon_start=1
                     /locus_tag="yruck0001_11780"
                     /note="similar to Putative uncharacterized protein of
                     Yersinia UniRef RepID=A4TKF1_YERPP"
                     /product="hypothetical protein"
                     /protein_id="EEP98520.1"
                     /transl_table=11
                     /translation="MHNAEDGDYQSLNEKMTEAGFTRLIGREKVYKLPDGEYNYGSGSL
                     NKQEVADLALQVAEKIRFVPSILVTESGGRTWRNLDSK"
     gene            complement(13929..14783)
                     /locus_tag="yruck0001_11790"
     CDS             complement(13929..14783)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089:methyltransferase (Score: 55.5; E-value:
                     9.8e-17)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="yruck0001_11790"
                     /note="similar to 23S rRNA methyltransferase A PRK11088;
                     similar to rRNA (Guanine-N1-)-methyltransferase of
                     Gammaproteobacteria UniRef RepID=A1JM68_YERE8"
                     /product="rRNA (Guanine-N1-)-methyltransferase"
                     /protein_id="EEP98521.1"
                     /transl_table=11
                     /translation="MSYQCPLCHQALQLNQQQWRCSNNHQFDCAKEGYVNLLPVQHKGS
                     KQPGDSPEMMQARRAFLDAGYYQPLQQRVAALLDERLSSEAKILLDIGCGEGYYTAAVA
                     ERLNQQRTMQVYGLDVAKVAVRYGAKRYPSVNFCVASSHRLPFADASLDAVLRIYAPCK
                     AEELARGVKSGGIVVTVSPGPRHLYQLKEIIYPQVQLHAENDEQLSGFESISSESLAYP
                     MILPGAQAMNLLQMTPFAWRASIEMGQALAAMDNFACETDFIIRVYQRQAEAVELSEKP
                     VTW"
     gene            complement(14881..16590)
                     /locus_tag="yruck0001_11800"
     CDS             complement(14881..16590)
                     /codon_start=1
                     /locus_tag="yruck0001_11800"
                     /note="similar to Peptidoglycan synthetase ftsI of
                     Enterobacteriaceae UniRef RepID=FTSI_BUCAI"
                     /product="Peptidoglycan synthetase ftsI"
                     /protein_id="EEP98522.1"
                     /transl_table=11
                     /translation="MISKVNVETNNFIRWRFSLLCGCILLSLLGLLGRVAWLQVVEAES
                     LAKEENLRSVRVMTTPNMRGMITDHNGQPLAVSVPVDAIWADPQVVLDKGGVGKGPRWQ
                     ALAQTLHQSLTEIASRINQSPTGRFVYLARQVEPTSADYIQRLKLPGIALRAESRRFYP
                     AGATVANLVGFTNIDDQGIEGIEKSFNQLLSGTAGHRVVRKDRYGHVVEDISATDSHPG
                     QNVQLSIDERLQTEASHALTNAVLFNKAESGSAVLVDVNTGEVLAMANYPTYNPNNRAD
                     TPTENFRNRAISDIFEPGSTVKPMVIMTALQRKLVKPDSVLDTHPYTLSGHQIRDVGFY
                     PTLSLTGVLQKSSDVGVSRLALAMPASALLQTYGLFGLGKPTELGLTGESSGLMPQRQR
                     WSDLDRATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVDPPVVGHRVMLAERVK
                     EVEYMMESVALPGGGGVKAAVRGYRVAVKTGTAKKIGPNGQYIDKYVAYTAGVAPASQP
                     RFALVVVINNPQGGKYYGGAVAAPVFSEIMGEILRTMNVEPDALPTQALRHS"
     gene            16802..17542
                     /locus_tag="yruck0001_11810"
     CDS             16802..17542
                     /codon_start=1
                     /locus_tag="yruck0001_11810"
                     /note="similar to Putative uncharacterized protein of
                     Enterobacteriaceae UniRef RepID=Q74V46_YERPE"
                     /product="hypothetical protein"
                     /protein_id="EEP98523.1"
                     /transl_table=11
                     /translation="MTDVSSIFTPIPGILHGFGSKTALLPVVLQPFRTSLPEKKQVHGT
                     RIVDVTEPNQICGEADGLYTTRPGVLLTVLTADCLPVIFSRRDGKAIAVAHAGWRGLLD
                     GILQKMAERIAREDSLNDWVVAIGPAVGSCCYEVTEQLVAQFIEKMSVPATVISPRYRH
                     LNLTAIAEYQLQSLGFYQVDRVGSCTLCTLAETVSVQSVSGEQIEAIPFKYTSFRRNSN
                     QRARDPSHPGISGRNQYSGLIIQP"
     gene            17651..17842
                     /locus_tag="yruck0001_11820"
     CDS             17651..17842
                     /codon_start=1
                     /locus_tag="yruck0001_11820"
                     /note="similar to cold shock-like protein CspC PRK10943;
                     similar to Cold shock-like protein cspE of Bacteria UniRef
                     RepID=CSPE_BUCAI"
                     /product="Cold shock-like protein cspE"
                     /protein_id="EEP98524.1"
                     /transl_table=11
                     /translation="MDFELYVLAFAQGFEAVTLDCREVYKHIFAAVSWSDETETFRLVE
                     PLNLTFNLSHLEYFLWIV"
     gene            complement(17857..17973)
                     /locus_tag="yruck0001_11830"
     CDS             complement(17857..17973)
                     /codon_start=1
                     /locus_tag="yruck0001_11830"
                     /note="similar to Uncharacterized protein yobF of
                     Enterobacteriaceae UniRef RepID=YOBF_ECO57"
                     /product="hypothetical protein"
                     /protein_id="EEP98525.1"
                     /transl_table=11
                     /translation="MCGIFSKEVLSKDVSVEYRFSADPYLSASSSNDSSLSM"
ORIGIN
        1 catgaatcta tccgcaacgc ttattctggc ttttgccatg tcaatggatg catttgctgc
       61 ctctatcggc aaaggtgcca ccctgtataa accccgtttt cgtgaagcta ttcgtaccgg
      121 attaattttc ggcgtgattg aagctattac gccgttaatt ggctggggta tcggcctatt
      181 tgccagtcaa tacattatgg aatgggatca ttggatcgcc tttgctctgc tgtttattct
      241 cggctgtcgc atgatcattg aaggctgcaa agggcccact gaagatgaac cgcaggtcag
      301 acgccacagt ttttgggtgc tggtcatgac cgctatcgcc accagtctgg atgcaatggc
      361 tatcggtgtc gggctggcgt tcttacaggt taatattcta cataccgcca tggcgattgg
      421 catggcaacc atgattatgg ctacgttggg tatgctgata ggtcgctata tcggcccgct
      481 gttggggaaa cgagcagaaa ttattggtgg tatcattctg ataggtatcg gttttaatat
      541 tttatacgaa catttatata aactcgccta aaccgcagac tattcctcgg cagccagcac
      601 gtgaatcgat gatatgacac cgaagggaga tatcattacc ctccggcgta gggggcatca
      661 aaggcgttcg atcagaagac tcaccaaggt gctggctgac ggaagcgaag gatgactcac
      721 cttctttccg agcgccgtat cataattctc gtgccactcc ggttctaata cctcgtagcc
      781 atcttccctt agttggcgga tattacgctg tactgcgggt ttatcccaca tgacagcccc
      841 catcacaggg aaaaacaaca cggggaaatc ggatgccaga atcaccgttg tcagacggtt
      901 aggcgcagcc ccttgcgcgg catcagccag ggtgttcgcc gtggcaggca gaaccaacat
      961 aatgtcatga tcagccacga tctttgacgg tttatctgtt ggccaatccg taggtcgctc
     1021 acccgcaatc acccgatcag cccacaacct catggtttca gccggaataa atcctgtggc
     1081 ctgcggtgtc atgagcaaag tcaacgaaca gtcaatgctg gccttgatcg cgcggaggta
     1141 accgggcaga agtgtaatat ccaccgatcc ggttgcgccc accagaatgc gtttcttttc
     1201 cattatcttg acctttattc gtgtggcagt aaggggtgaa cgcaaggttc acccactgat
     1261 ggacttaagg cgtacccgga ggtgcctcac cccagcccca aagtttgacg gtttctcgct
     1321 tcgaacgctc ggccttcggt tgggagttag cctgagcacg gcgggaacca aactcaatat
     1381 aaccggcaaa ggctgaacga aactccggat cagcagggag attcacctcg tcagcacatt
     1441 ccatcatcaa gtccacccag cgcttacgct gctcaggttg gatagcccta ccacggtgct
     1501 tggccaccat gcctttaaaa ccacctcggt gcgcggtata gtttgcctcc ccgcccagaa
     1561 cttcttccag ccagatagcg acatgttctg gatgatcggc atgcatgtgc acaaacatgg
     1621 gtgccagcag ttcatctttc tcgatttttg cataaaagac ggtcattaac ttcagcagga
     1681 cttctctacc gcccatccat tcaaacaatg taggggtttg ctcggacatg atttttctcc
     1741 ataacatacg gatttttgta ccgatagggc tgaatctcaa cccaattcga gtgaagctac
     1801 agcattgttg cctttttgat agctgacagg gggggtgccg cgatacatcc tgcaagtatg
     1861 aaaaagcggg ctaaatccag cgactttcag aacatcaaga gtggcacccg cctgctctgg
     1921 gacatcaagc gtcaccagag cattcttcgg taaagctccc agcgctgttt gcagcaatgc
     1981 aatggcatga ttttcatccg gggcatagaa aggcccgatg cgatatccct catccgaggg
     2041 acgcaaggca atgacacctt ttactgatgc gccttccgta accacgaatc cctgacggtt
     2101 atcagcagta aaccaagagg acaacaggct agcccggtta caaccggaaa acgaggtatc
     2161 aaaatcagca atagcagaaa gatgatggct gctcacctcg ctgatattgt cagccttgat
     2221 ggcaatacca gaggcccgcc cctgaatccg ataggtgtga taatgagtac ggaatcccca
     2281 ttttttgtag ttttcctcct gcatcggcat accgtccaga ccgatacaac gctccccggc
     2341 atgttcgatt gccgcagtcc acagctcaag acccaggcct tttccccggt attctggtgt
     2401 aacaatatag tgtcccaagt gggcatagcc tggatcaaaa ttcacaatag aaattgaggc
     2461 aacaatccgc ccctcggtac ggccaatgaa aaaaccattt ttatccacat tcaggaaaat
     2521 acggtcatca ccatgaccca aatcccagtg ctcgttacgg gccatttcaa ccgcgctttg
     2581 ccattcagcg ggcgtggcaa ggtgtacact gaatgcctta tcggtgcttt tgactacatt
     2641 tttcatcact catctccaga aataatggtg caatcaaagc cccatagact tactgataaa
     2701 ttctttgagc acttcactgg ttccgccgta aattgtctgg acgcgactat ttacccaagt
     2761 ctttccgatg aaggagtcac tgagataacc cgcgccgccg tgcaactgca tacactgatc
     2821 ggcaacctgg atctgtagct cggtggtcca aagtttgatc cttgccgcat cggcaacgtt
     2881 aagctcgcga cggttgaact tatcaatggc atcgttcaga tagatccgtg caatcctcac
     2941 attggtatcc aactctgcca gccgaaacct gttgtgttga aatgacccca cgggttgacc
     3001 aaaagcgtga cgttttttaa cccattcgag cgtgcgttcg aggagcagtt cggcggtagc
     3061 aacggccacc gtcgagatgc tcagtctctc ccttggcata ccgcccataa aataaaagtt
     3121 acctatattt tcctttccta tcaggttatt acagggaact agacagtttg aaaaatgaag
     3181 attggcagta tcactggcat gccaacccag ttttttcagt ggcgctcccc gcgtaaatcc
     3241 tgctgtatca cgttcgatga ccagcaaact gatgccccgc ccgcgctctg ctttcgatgt
     3301 tctgaccgcc gtcaggatca gatcggcatt gatgccgtta gtgataaagg acttttgacc
     3361 gttgacgaca taatgctgat cctgcctgac agccgtggtt tccaaatcag ccgagtcaga
     3421 acccccgtta ggttcagtga ccgcgatggc agcgatcaac tctcccttac acagccctgg
     3481 cagccagcga tcgcgctgtt cgtcggtgcc atgtgcatca atgtaactgg caatcacatc
     3541 attgtgtgca atcacgactg gcgccgtcat ttcagcacgg atcaactctt cagtgagcac
     3601 tgaggcatat tgataatcgg cttggttctg cccgccccat cgcccggata caccgatgcc
     3661 cagaatccct gcctcacccg catgtcgcca aaaatcacga ctgacgaggc cctccttttc
     3721 ccatacggaa agatagggtt tagcctcatg ttccagaaac gtccggcagg atgcacgaaa
     3781 cgctgataat tccttgttat ccatctaaac ctcagaactc ttatgcagga attgacacat
     3841 tgtgctcaac tgaagattgc gagagcagcg tgcggacggt gttaaaattg tcatagacag
     3901 caaaatggcc accctggatc tcggtgatac tgacgcgaga tcgtgtgtgg ttaagccagg
     3961 ctaactgcat ttgcgcggtg acatgctcat ccttatccgc agaaatgagc aatagtggct
     4021 gctccacacg catatcccgg tatgccaaga attgctgacg caagatgtag tcagttcgta
     4081 gtgctgtcac tagccggcga gcgatggctg gttcgcgcag gatactttca ggtgtgccgt
     4141 tatctgcgcg cattttctcg aggatttttt cgtcagacca caagtagcga tcatcttccg
     4201 cctgagccgt cggtgcaatg gaaccggaca ccactgaaaa caccgggttc atagcgtatt
     4261 tatctgccag cagatggcta acgatgacac tcaaaacacc accaaagcta tggccgaaag
     4321 tggcaaactc tgtgatgccg gcttttttgc gctgggtaaa atccgcgtag atatcggcgc
     4381 tgatacggct cgccaacacc gccaggtcat cgatcacttt ttcacgactg agtctgtccc
     4441 gacccggaat cccaacggga ctgaatacca gtttactttc atcgttaaaa cggctcagcc
     4501 aatgtcggta aacaccggta cccgcaccag catgagggaa agcgtaaacc actcgcccgg
     4561 atgataaatt catcatacat tctcctccat taagaatgag gcgattcgct ctgccaccat
     4621 cgccgtagtc agattcgttg gcatagaagg gatgcaaggg aagagtgagg catcggccac
     4681 cgtaatatta cggagcccat gaatattccc ttttttatcg gctgcacttt ctggatcggc
     4741 ggaagttccc attcggatgg tgccggaggc atgccatcca ggactgacga ggtttatgat
     4801 ggcattgcgc atcacttcat catttcccat cattgattcg cgccagaact ggacgccctc
     4861 caggcagaca ctgacctctg gatgattgat cagttgccac atttggcgca cgccaccgac
     4921 aagccgctga acatcctcct cattttgcga cagaggcaga tcaatcgcgg gcagtttatc
     4981 gctgtcccta gagcggataa acactcgccc tttcgcttta ggccgcatca gcatgaccga
     5041 tgcaccgacc agaaaggggt attctgtacg gttatggaag ccaggcacgg tgtcgctggc
     5101 cacgttgttc atcaatccca gttgaacatc aagttgctca tcatagccac tgctcattcg
     5161 agccgccgtt tgccgccaag gggttttcac cttactgttg ctggctcggg gaagcgccca
     5221 catcaccact gaggcgtgat cagataggtt gtttcccact gcagggatat ccgccacgac
     5281 cggaatatcc agtttatgca gcagttcgct atcacccaca ccagaacgct ggagaagagc
     5341 ggcggaaccg atagcaccgg cacagagtac gacccggctc gcagagagtg tctggtattt
     5401 tccatttctg tgtacttcaa caccagtggc tgcgccatta gcaaaaagaa cgcgttctac
     5461 agtcatttcc gtcatgatct gaagattact gcgcattcgg gctggagtga gataagcccg
     5521 acaaaaatca aaccgttctg cgccctgaag cacattggcg ggcaccgcac ctactgccgc
     5581 gtcctcaccg gtgttaaagt cctcaaccca aggaatgccg tgactgacgc aggcgctggc
     5641 aaaacccgtt tccaacggta gagtttcttc cgcagtaggc cgccgcaaca gcagtgggcc
     5701 agcatgcccg tgcagtgtct cgtccgcgat atcacggtca ttttccagct ttcggtacca
     5761 aggcagcaca tcctgccatg accaagtcgg gcatcccata tcgacccagc acgcaaaatc
     5821 tcgtgggaaa gcacgcaacg cgacagcacc gttgattgcc gatgatcccc ccagcacttt
     5881 ccccagccga taaccaaaag gtttccttct gtcacgcgga gcatgagatt cggcatccgg
     5941 ttgtaaaaag cgcgacagcc gttcggcacc gcgtacattg gcttcatatt cccagttata
     6001 gttttccagt accaaccgcg aggcatcgtt cagcggactg ctatggtcgt cagcattgga
     6061 atcaaggcct gcttcaatca acaaaaccga cttcgcgcta ttttcagaaa gccgggatgc
     6121 cataacacat cccgcagtgc cgcccccgac cacaatatag tcatagtggg acaatcccat
     6181 atcctgactc atgacaaggc ttcctcagga tcaagggtca cttccgcaag aatttttgag
     6241 aagtcgcgca ccgaagcatt cataaacaga ctttcgatca tatcctgctc gctggacgga
     6301 ctgacgcgga aagactgacg aaccgcgctc aacaggctta ccgcatgaag cgaatcgccg
     6361 ccaatatcga aaaagccgtc agttacgcca aaatcgctgt gctgaagctg tgccgcccaa
     6421 attgaataga ggcgggtttc cgtctcatcg gcaggtacat tccgactgtc ttctgccaca
     6481 ctatcagccc ataccgcagg caacgcgttg cgatcaatct tgccatttgg ggtcagaggc
     6541 atttcatcca gcagaacaaa ccacatcggc accatatagg atggcagcgt cgccattgcc
     6601 gtttcccgaa gttcgttttc aagccggttc ttgtcatagc cagcagaatg agtggcaaca
     6661 atgtaagccg ccaactgttt ctgccctgat ttaggatgcg tcaccgcgtt gatcactaca
     6721 tgactggccg atggatgttc cagaagtgcc gcctcaatct cgcccagttc aatgcggtag
     6781 ccattaatct tgacctgatt gtcctcacga ccaaggattt caataagacc ctcgttgata
     6841 tagcgaccaa gatcacccgt tcggtaaagt ctctctcccg tccgaggatg ctggaaaaag
     6901 cgcgcctggg ttttctcatc gtcacccaga tacccttgag caacgccatc accagcaata
     6961 aaaatctcac ctgtaaccca tttagggcta ggttcaagcc actcattgag tacatggaat
     7021 cgttggttag cgagcggctt accataggga atacttttcc aggtggtatc caccgtttgt
     7081 accggataga caatcgacca gatcgaccct tcggttgcac ctcccagact gatgacgtca
     7141 attttctctg aaacatggcg aatttgatcg ggaaggtcga caggtatcca gtctccactc
     7201 atcagaacaa gacgcagtga actgtctctt agttgttcac ccgcccgctc aaacagcgct
     7261 tttaccggtg cagggacagt gttccagagc gtgacgccct gtttaacgat ctgttcagcc
     7321 caaagaccgg gatcggctgg ctcgttctca gcagcaaaga gaatgctgcc accggcacca
     7381 agtacgccga agtaatcata aacagagaga tcaaatccag caggtgcaat ggaatagact
     7441 cgatcctggg aattaacact aaaccgtcga ttgatgtcag caactgtatt gactgcattt
     7501 gtatggctga tcatcacccc tttgggttcc ccagtagagc ctgatgtaaa aatcacatac
     7561 gccaggtcat ccggtgactg tttacgcggc gttgggggca aaggacggag agtgcccgaa
     7621 acgatagtgg tcacaacaat gaccatacct tgtaccgcga cgttaccatc aaaggtcagt
     7681 acggctttcg cggcacagcg ttcgataagg cgatgttgac gttgctctgg caactgcgga
     7741 tccacagaaa catatgccgc accagcaatc agcaccccca ggatagccgc cagcatttct
     7801 gggctttggt gaagcgagac tgcgatgata tctccgggat tgatatccac ctgcgcatga
     7861 agctgtgtgg caatatcatg agcggcggtc actaactcac ggtaactcac ttcacgcgtc
     7921 ccttgtagca cggccgtttt gtctgggaag cggcgggcag catccaggag ggactcatgc
     7981 aggagcgtcg gtggcaagtg atactgtgtc gcattagcgt ccagacgtgc cttttcatca
     8041 gcctttggca acgtcaccat gctgtgacgc tgggaccaga ctgacgggtt atctgcacaa
     8101 gcacaaagca acttgtctgc cgcctcaaac atcgcctcgg ccatgccatc agggaaaagg
     8161 ccgtcaacat aattccagtt catgaccagt tcaccattaa tgcgcatcaa ctggttttca
     8221 agccaaacac ccggcgtgcg cgtggcggta tgaacgatac gagcagcatt tcgccaaaga
     8281 tcatcagaaa caatgtcttc caactcggca ttcagcgtat tactgaatac gactggcagt
     8341 gagaatgctc ggccatcgct attgagtcgg gtcatttcac gtaagacttg aatgccatta
     8401 aaagatgagt gccaacgatt aagcagcagt tccgtctgta gactgaccag ccgttcggcg
     8461 aacgagtcag aggatgggct gttcacacaa agcagacatg actgtaaaaa attacccgta
     8521 atatgagtaa tgtcttctga ataggcacgt cggccatttt gggtcagggt gagcgtgaaa
     8581 tcctgacggc gggaccattg acgcagcact tccgtataga cgccgaggaa cagcacttcc
     8641 agcgtgatgc cattttgagc cgcggcatgc cggagtttgt taatcgtctc gaacggtatt
     8701 tcttttttca gggttttaat gacgggatgt ctgattaact ccggtgattg gtgaagagga
     8761 agttcaggtg cacccggaat atttttaatt ttattgatcc agtatgcttt gtcccgctct
     8821 ccctgttggc ttttctggag ttctttttca agagagatat agtcgctaaa attaaggcga
     8881 ctctcagttt tttgcatttc gggattttca taaaactccc accagtcacg cataataatt
     8941 ttaatactgt gtaaatcgaa aaacattaaa tcaaaacaca ggtgaagtct gatatttcta
     9001 tttcctatct gggttgcacg aatatcaaac ggacgtacat tgccttgggt tcgcatggct
     9061 tcaatcattt cgatacgggt ctttccgacc tgatcatgca ccgactgagg atcctgtcca
     9121 ctacagtcaa ttaacgggat aacatcatca cccattgcgg gtaaaaaacg ctggcgcccg
     9181 ctatctgtca gggctgaacg cattaccggg tggcaagcaa taacgcggcc taatgcatca
     9241 ttcaatcggt caaaatccgg gttataaacc tcaatttccg tataatgaga agccggtaca
     9301 ccgcccaatg ggatcgctgc attacggcca atcagataag cgtactggat atcggtaagg
     9361 gagaaaggtt cgctatcatt ttcttctctt ctgttatcag aataatttat ttcgccagcc
     9421 ggaagaatcc cacacattaa tgctgtcagg ttaagtgcaa cattatcgct atttgaatca
     9481 tactgaagga caattggcac atcattatct agagaaaggg tcagacctaa tttatcggta
     9541 gcttcccgca tcattttcat aattcactcc actcatctgt gtttacctat aaataaccaa
     9601 agaagacagt gtttagttac gtttaggccc gcttgaatta atgttacgtc cctgattgat
     9661 aaaatttttc taatagatat aaagttagcg atagataaag tatcgccaag ttgcccagcc
     9721 agtaattatc agaaagcctg aacagatatg tcgttttatt attcaaacac tcaataagaa
     9781 cattgagtgt ttgaatcgta ccgcgcactt tggattgcgt caagatttaa taacattaac
     9841 tggtaatgta aataaccatt tattaacaaa aaaatcacgc ttaggatttt tattgcaact
     9901 cactgtattt tatataaata ttttttaatc ttgtttagtc accccgccaa aagtagccaa
     9961 aaagttcttt aagataaata ttaatataaa taaaatttat cattcatcta attcccacat
    10021 gacccatttc gttgacaact gatcaatcat taattcatta ttcaacctat cgcttgaatg
    10081 atcatgctgg ttattccacg tattactttg gttattatat taattttcac ccgaatcagt
    10141 tacctgtctg tattagccac actaatcatt caatgtgtca attgaatgat tgtattaatt
    10201 gaattaatca ttcaatatat aagcggaatg actatttaac ggatgctgac ggattcccac
    10261 gattccaagc tctggactga cagtcgctaa actctggttc aatgaggtat tcatatgaat
    10321 gatggattta ctgtagtaac agacaatgct gccgcatctc acaatagaac gtcaggatcg
    10381 caggatgctc cggtgaacaa tgtcaggtta ctgttttcaa ccctcgccgt tatccaagcg
    10441 acgctgattt ttactattgt catgatcggt gttccgctac cggctattgg gacggaattc
    10501 gggctggttc aagcggaact ggttctggtc agcgctggct acgggctgcc attcagtgga
    10561 ttgctgctat tgggtggccg attgacagac cgttacagtg gaagaaaaat gttcacctta
    10621 ggggtgatac tttttggtac ggcctcgctg tatgggatgt ttgtacccga ctattggaca
    10681 ctcgtggtag tccggttcat tcagggaaca ggggcggcaa tgacagcacc agcactgatg
    10741 gcactgttac gccagttgta tcctgattct gctgatttcg gacgcgccat ggcaacctgg
    10801 ggcggtgtct ccgtgcttgg gggagccgca ggttttctct catccggtat tgttacccac
    10861 tggctatctt ggcgctggat gttcatcatt cccgtggtgg tggcattcgt gggcttggta
    10921 attgtacgta aagtcatggt agcgaccgaa gagaaaacat ctgcccagcc tgggctagat
    10981 cttggcggcg cattactcgc cagcagtggt atttccctgt tcagctttgg cctgattttc
    11041 agcggtgaat atccgtggtc agcccccatc gtctggggct ccgtaatggt cggactgctg
    11101 ctgttcgttg cgttccttag cgttgaaaaa agggtgtcat ttcccttact tcctccgcga
    11161 tttatccaca atcccgcccg agctatcggc ctcgcgggga ttttgctggc tgcggcagga
    11221 atggggctaa ttacatttct actgtcccta tatctgcaac aattccaagg ttggccaccg
    11281 ctagccacct caatggcatt tattccctat acccttggtc tactggtgat gaatcgggca
    11341 gcgggaacac ttattgcacg ttatcacgcc ctcaccgtta ccattgtcgg actcttaatc
    11401 agcgcagtgg gactgtttct tctggcaatg atcaacccag aaacggatta catgaccgga
    11461 ttactacccg ggcttttcat tctccccgca ggggcttcat tggtcttttc aggcagcgct
    11521 gtgctcgcca cagccaatat tcccctacac caggcagggc tggccggtgg tgtgatgaat
    11581 acggcgatgg aactgggtcc aacaatgggt ctggcggcat tgatggcagt ggcggctacg
    11641 caggtcgatg tcattgacgg atatggcttg gcttttggta cggcgggctg tatttatgtt
    11701 gtggccgcga tggcagccat tctctgttac cggaacatca aatcaccctg ttaatactta
    11761 ggccatggcc tgcctcaggc catggcgatt tattacagca ctggggacgt tcagcgatga
    11821 ttttgtactt caagtcccgc cgctttccac tcgggaaacc catcttcaag gcagcggaca
    11881 ttgtaaccat gttcccgcag caccttcatc gcgttaaccg aaaggacaca gtatgggcca
    11941 cgacaataag caacgatatc ctgagctaga ggtaactccg agaggcgccc ctcaagctgc
    12001 tccacaggaa tatttattgc accaggcaaa tggccgtgac tgaattcctc ataaggacgc
    12061 acatccagca aggtgacatc accttcgttg aggcgattca gcaattcctc acgcgaaacg
    12121 gactcaaaac gctcacgctg atttaaggtt tctgacacaa agttactcat ttcattatgc
    12181 cgatattcca catattggcg aatggccgtc aacaaattct gcactggccc acggccaagg
    12241 ctgtagagca cattttttcc atcacgacgt gcctgaacga atccaacacg tttgagatgc
    12301 tgcaaatgct gggaggcgtt ggcaatggat atgttggcca actcagccag cctttccacc
    12361 gagcgttcac cttgggaaat atgctccaga agaataaggc ggtgtgcatt tcccagggag
    12421 cgtgtgacct ctgcaagttc atgaaacact gggtggttaa tgttctcgtt gttggttgac
    12481 ataggttaat cattacccca ttattcaatt tattaattga aggttatctt atcaaaaccg
    12541 tacccgacca gccagcggga accagactaa attgtcaccc tctcggtgag tataggaagc
    12601 ctgtaatctt atgattgatg aaaaaggaag ctgaaatgga tattacgtta caagcagtcg
    12661 ctattggaat cggggcaacc ttaattatgg atatctgggc gatcctgcaa aaacgcttgc
    12721 tcaatattcc ttctcttgat taccggctgg tcggacgctg gttaggtcat atgtcgcggg
    12781 gacaattcgt gcatcacacc atactgcagg caaacgcagt caggggagaa gtgctggtag
    12841 gctggctggc acattatgct attggcatca ttttttcatt tttactgatt accgccaccg
    12901 gccccggatg gcttgtagaa ccaacctttc tccccgcgct gattgttggc atcatcagca
    12961 tagtggctcc cttttttatt atgcaaccgg gttttggctt cggcatcgcg gcctctcgtc
    13021 tcccgcaacc taatgtggcg cgccgacgta gcctaattgc tcacacttca ttcggtgtcg
    13081 gtctttatat tagcgcccag gttctacgct tcatgctaaa ctaatgatcc agaacaacga
    13141 gtgagattaa taacacccgg tgtcacagcg gtgaaaaccg acagagcgtc agagttcaag
    13201 ccatatatca gtctgatatt gtcggttatt tagccaacca tacctaaccg tattcattga
    13261 attttcagca attcgatctc ggtcataata aaaaacatag cgtcatgata tacagaagat
    13321 tattccgtaa aaatccaaag gatatctatg ccttacttta ctgtgagtgt tagattgcat
    13381 aatgctgagg acggcgatta tcaatctctt aatgaaaaaa tgaccgaagc tggatttacc
    13441 cgattaattg gtagagagaa agtttataaa ttgcccgatg gtgaatataa ctatggtagt
    13501 ggctcattga ataaacagga agtcgccgat ttagcattac aagttgccga aaaaatccgc
    13561 ttcgtccctt ccatcctggt gactgagtcc ggtggacgca catggagaaa tttggattca
    13621 aagtaacagt attattaaaa accctttaaa tacataatca taaaagcaca ttcacttgct
    13681 gacaatcaag ccagatagcg ccaacttatg tttgcgctct ggctagcaga ttaattatat
    13741 cttctgtact ctttacgaaa aaaatagcat taaacaatga ttactgacaa tttaacttaa
    13801 atgccaatat tccgcttatt attatgaaga gcaaaaatca aattattatg tttaatctga
    13861 ttctcgccgt tatatcagtg aaatatcatg ttgccgctat ccgctatccg ctattcccca
    13921 gttcaccatc accacgtcac gggtttctct gatagctcga cggcttcggc ctgacgctga
    13981 taaacgcgaa taataaaatc cgtctcacag gcgaaattgt ccatcgcggc caatgcctgc
    14041 cccatttcta ttgatgctcg ccaagcaaac ggcgtcatct gtaacaaatt catggcttgc
    14101 gcgcccggta aaatcatcgg gtaagccaag gactcactgc taatcgattc aaaaccggaa
    14161 agttgttcat cattctccgc gtgcaactgc acctgcggat aaatgatctc ttttaattga
    14221 tacaaatggc gtggtcctgg agacaccgtc accacaatgc ccccgctttt caccccacga
    14281 gccagttcct ctgccttaca gggtgcataa atccgtaata cagcatccag tgaagcatcg
    14341 gcaaacggca atcgatgact ggatgccacg caaaaattga ccgacggata acgtttcgca
    14401 ccatagcgta ccgccacttt cgccacgtct aaaccgtaaa cctgcatcgt tcgctgctga
    14461 tttaaacgtt ccgctactgc agcggtgtaa tagccttcgc cacagccaat atccagtaat
    14521 attttggctt ctgacgacaa acgctcatcc agtaaggcgg ccactcgctg ttgcaatggc
    14581 tgatagtaac ccgcatccag aaacgcacgt cgtgcctgca tcatctctgg actgtctccc
    14641 ggctgttttg agcctttatg ctgcaccggt aacaaattga cataaccctc tttggcgcaa
    14701 tcaaactggt gattattgct gcaacgccac tgttgttgat taagctgtag cgcctgatga
    14761 caaagaggac attgataaga catggttata tttccaaatg gcagggaaaa actaaggcgg
    14821 cgctagtcta aagaaccccc cacgccgttg caagccggag cgactcattg tgcttcaatc
    14881 ttagctgtgt cgcagcgcct gggtaggtaa agcgtcgggt tcaacgttca tagtacgtaa
    14941 gatttccccc ataatctcgc tgaataccgg agccgctacc gccccgccat aatatttccc
    15001 tccctgcgga ttattaatca ccaccaccaa cgcaaaacgt ggctggctgg ctggcgcaac
    15061 gccagcggta taagccacat atttatcaat gtattgtccg ttaggaccaa ttttcttcgc
    15121 agtcccggtt ttgaccgcca cgcgatagcc gcgaacggcc gccttcacac cgccgccgcc
    15181 gggtaacgcc acgctttcca tcatatattc aacttctttt acccgctcag cgagcatcac
    15241 tcgatgaccg actaccggtg gatctacttt ggtaatcgat agtggtcgat aaataccgta
    15301 actgcctata gtcgcgtaaa cccgagccaa ttgcagtggt gtaaccatca aaccgtagcc
    15361 gaatgagaaa gtggcacgat ccagatcact ccaacgctgg cgctgcggca tcaatccact
    15421 gctttctccg gttagcccca attccgtcgg ttttcccagc ccgaataatc cataggtttg
    15481 cagcaatgct gaggctggca tcgccagtgc caagcgggat acaccaacat cgctggattt
    15541 ttgcagcaca ccggtcaacg acagcgtcgg ataaaaacca acatcacgga tctggtggcc
    15601 actcaaagta tagggatggg tatccagcac gctgtccggc ttgactaatt tacgttgtag
    15661 cgccgtcatt atcaccatcg gcttaactgt tgaaccgggt tcgaaaatat cgctaatcgc
    15721 ccgattacgg aaattctccg tcggcgtatc cgcacgatta ttcggattat aggttgggta
    15781 attggccatc gccaaaacct cccccgtatt aacatccacc agcaccgccg atcctgattc
    15841 agccttgtta aataacaccg cgttagttaa agcatgggaa gcctcagtct gcaaacgctc
    15901 atcaatgctc agttgaacat tttggccggg atggctgtcg gtggccgaaa tatcctcaac
    15961 cacatgacca tagcgatctt tacgtaccac ccgatggcct gcggtgccgc tcagcaactg
    16021 attaaaactc ttttcgatgc cttcaatacc ctgatcgtcg atattggtaa aaccgaccag
    16081 atttgccacc gtcgcacccg caggataaaa gcggcgggac tctgcccgta aggcaatacc
    16141 cggcaatttt aagcgttgga tgtaatcggc agaagtcggc tccacttgtc tggccagata
    16201 aacgaaacgc cccgtggggc tctgattaat ccggctggca atttcggtca gcgattggtg
    16261 cagagtttga gctaacgctt gccaacgtgg gccctttccc acccccccct tatccagcac
    16321 cacttgggga tcggcccaga ttgcatctac cggtacactg acagccaacg gctggccatt
    16381 atgatcggtt atcatgccgc gcatattcgg cgtggtcatc actcgaacgg aacgcaggtt
    16441 ctcttccttt gccaaggact ccgcctcaac aacctgtaac caggccaccc gaccaagtaa
    16501 gcctaatagt gataaaagaa tacagccgca caacaaacta aaacgccagc gaataaaatt
    16561 gtttgtttca acattcactt tagaaatcat ccattttcct gtggggttcg ccactttggc
    16621 tgctcattaa acccgatcca gggtacctac agggaagaaa cagtgataca agaccactta
    16681 ttttgcaaca aagcgcaagc aaccacgtta ttccgatgaa taaacccaca gccagtagcc
    16741 agaaagggaa aaatcgtcct acactgagcc attctccagc cacaaaggcg atattattta
    16801 catgactgat gtatccagca tattcactcc tattcccgga atcctccatg gttttggtag
    16861 taaaaccgcg ctattgccgg tggttctgca accttttcgc actagtttgc cggagaaaaa
    16921 acaggtacat gggacgcgta ttgtcgatgt cacggagcca aatcagattt gcggtgaagc
    16981 tgatggcttg tataccactc ggccgggggt gctattaacc gtgctgacag cggattgtct
    17041 gcccgttatt ttcagccgtc gagatggaaa agcaattgcc gtcgctcatg caggctggcg
    17101 cggtttactg gatggaatac tgcaaaaaat ggccgaacgt attgcgcgtg aggattcatt
    17161 gaacgactgg gtggtcgcca ttggcccggc ggtgggttcc tgctgttatg aagtcacgga
    17221 acaactggtt gcacagttta ttgaaaaaat gtcggtgcct gccaccgtga tctcaccgcg
    17281 ctaccgtcat ctcaacctca cagcgattgc cgaatatcag ttgcagtctc tcggatttta
    17341 tcaggttgat cgcgttggca gctgtaccct atgcactttg gcggagactg tctcagtcca
    17401 gtcagtatcg ggtgaacaaa tcgaagcgat accgttcaaa tacaccagtt ttcgtcgtaa
    17461 cagtaaccag cgagcacgtg atcctagcca ccctgggatc agcggcagga atcaatattc
    17521 aggcttgata attcaacctt gaacctgact cgacacagac gaaaaaaccc gcacgtaggc
    17581 gggttttttt tcagctagag ctgaattaga ttgcagtaac gtttactgca gacggacctt
    17641 tctgaccgtc ttggatttcg aactctacgt tctggccttc gcccagggtt ttgaagccgt
    17701 taccctggat tgcagagaag tgtacaaaca catctttgct gccgtcagct ggagtgatga
    17761 aaccgaaacc tttagactcg ttgaaccact taacttgacc tttaatctta gccatcttga
    17821 gtatttcctt tggattgttt aactcgcccg gaggcgttac atagacaaac tagagtcgtt
    17881 actgcttgag gcactaagat aaggatcggc agagaagcgg tattcaacgc taacgtcttt
    17941 actcagaact tctttactga aaatgccaca cat
//
