BGC0002097: kosinostatin biosynthetic gene cluster from Micromonospora sp. 28ISP2-46
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Location: 5,660,056 - 5,708,619 nt. (total: 48,564 nt).
This entry is originally from NCBI GenBank CP059322.1.

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core biosynthetic genes
additional biosynthetic genes
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Gene details
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General
Compounds
Genes
Polyketide
NRP
Saccharide
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0002097
Short description kosinostatin biosynthetic gene cluster from Micromonospora sp. 28ISP2-46
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Remarks "We rediscovered kosinostatin from a Micromonospora strain and identified the BGC in its genome sequence based on homology to the previously published BGC (antiSMASH). Certain genes thought to be important to kosinostatin biosynthesis are missing from our cluster."
Biosynthetic class(es)
  • NRP
  • Polyketide (Anthracycline)
  • Saccharide (hybrid/tailoring)
Loci NCBI GenBank: CP059322.1
Compounds
  • kosinostatin
Species Micromonospora sp. 28ISP2-46 [taxonomy]
References
Chemical products information
kosinostatin [synonyms: quinocycline b]
(no structure information available)
Chemical database entries
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • H1D33_25545
  • QLQ36600.1
 5660056 - 5661471 (-) UbiD family decarboxylase
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  • H1D33_25550
  • QLQ40534.1
 5661468 - 5662160 (-) transglutaminase domain-containing protein
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  • H1D33_25555
  • QLQ36601.1
 5662199 - 5663059 (-) methyltransferase domain-containing protein
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  • H1D33_25560
  • QLQ36602.1
 5663255 - 5664757 (+) FAD-binding oxidoreductase
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  • H1D33_25565
  • QLQ40535.1
 5664799 - 5665869 (+) beta-lactamase family protein
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  • H1D33_25570
  • QLQ36603.1
 5666004 - 5666441 (+) Ohr family peroxiredoxin
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  • H1D33_25575
  • QLQ36604.1
 5666454 - 5667257 (-) TetR/AcrR family transcriptional regulator
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  • H1D33_25580
  • QLQ36605.1
 5667340 - 5668176 (-) NAD(P)H-binding protein
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  • H1D33_25585
  • QLQ36606.1
 5668310 - 5668765 (+) ester cyclase
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  • H1D33_25590
  • QLQ36607.1
 5668768 - 5669811 (+) zinc-binding dehydrogenase
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  • H1D33_25595
  • QLQ36608.1
 5669882 - 5670865 (+) Gfo/Idh/MocA family oxidoreductase
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  • H1D33_25600
  • QLQ36609.1
 5670930 - 5672231 (-) hypothetical protein
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  • H1D33_25605
  • QLQ36610.1
 5672228 - 5675017 (-) ATP-dependent helicase
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  • H1D33_25610
  • QLQ36611.1
 5675143 - 5676177 (-) alpha-ketoacid dehydrogenase subunit beta
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  • H1D33_25615
  • QLQ40536.1
 5676197 - 5677138 (-) thiamine pyrophosphate-dependent dehydrogenase E1 component subunit alpha
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  • H1D33_25620
  • QLQ36612.1
 5677240 - 5678721 (-) NDP-hexose 2,3-dehydratase family protein
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  • H1D33_25625
  • QLQ36613.1
 5678721 - 5680016 (-) activator-dependent family glycosyltransferase
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  • H1D33_25630
 5680076 - 5680304 (-) hypothetical protein
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  • H1D33_25635
  • QLQ36614.1
 5681234 - 5681809 (+) dTDP-4-dehydrorhamnose 3,5-epimerase family protein
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  • H1D33_25640
  • QLQ36615.1
 5681871 - 5682323 (-) nuclear transport factor 2 family protein
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  • H1D33_25645
  • QLQ36616.1
 5682529 - 5683536 (+) DMT family transporter
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  • H1D33_25650
  • QLQ36617.1
 5683577 - 5685028 (+) MFS transporter
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  • H1D33_25655
  • QLQ36618.1
 5685096 - 5686196 (-) alpha/beta fold hydrolase
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  • H1D33_25660
  • QLQ36619.1
 5686309 - 5687142 (-) ABC transporter permease
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  • H1D33_25665
  • QLQ36620.1
 5687139 - 5688215 (-) ATP-binding cassette domain-containing protein
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  • H1D33_25670
  • QLQ36621.1
 5688384 - 5689172 (-) TetR/AcrR family transcriptional regulator
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  • H1D33_25675
  • QLQ36622.1
 5689244 - 5690140 (-) NmrA/HSCARG family protein
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  • H1D33_25680
  • QLQ36623.1
 5690164 - 5691006 (-) NAD(P)H-binding protein
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  • H1D33_25685
  • QLQ36624.1
 5691074 - 5691508 (-) ester cyclase
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  • H1D33_25690
  • QLQ36625.1
 5691542 - 5691799 (-) acyl carrier protein
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  • H1D33_25695
  • QLQ36626.1
 5691833 - 5693041 (-) ketosynthase chain-length factor
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  • H1D33_25700
  • QLQ36627.1
 5693038 - 5694306 (-) beta-ketoacyl-[acyl-carrier-protein] synthase family protein
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  • H1D33_25705
  • QLQ36628.1
 5694303 - 5694656 (-) antibiotic biosynthesis monooxygenase
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  • H1D33_25710
  • QLQ36629.1
 5694851 - 5695636 (+) SDR family NAD(P)-dependent oxidoreductase
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  • H1D33_25715
  • QLQ36630.1
 5695665 - 5696624 (+) SRPBCC family protein
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  • H1D33_25720
  • QLQ40537.1
 5696696 - 5697427 (-) SDR family oxidoreductase
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  • H1D33_25725
  • QLQ36631.1
  • rfbB
 5697520 - 5698518 (-) dTDP-glucose 4,6-dehydratase
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  • H1D33_25730
  • QLQ40538.1
  • rfbA
 5698695 - 5699573 (+) glucose-1-phosphate thymidylyltransferase RfbA
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  • H1D33_25735
  • QLQ40539.1
 5700281 - 5701162 (+) AfsR/SARP family transcriptional regulator
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  • H1D33_25740
  • QLQ36632.1
 5701246 - 5701944 (-) indole-3-glycerol-phosphate synthase
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  • H1D33_25745
  • QLQ40540.1
 5701977 - 5702633 (-) N-(5'-phosphoribosyl)anthranilate isomerase
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  • H1D33_25750
  • QLQ36633.1
 5702643 - 5703782 (-) acyl-CoA dehydrogenase family protein
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  • H1D33_25755
  • QLQ36634.1
 5703839 - 5704213 (-) ectoine synthase
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  • H1D33_25760
  • QLQ36635.1
  • trpA
 5704210 - 5705031 (-) tryptophan synthase subunit alpha
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  • H1D33_25765
  • QLQ36636.1
 5705024 - 5706064 (-) hypothetical protein
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  • H1D33_25770
  • QLQ36637.1
 5706067 - 5706330 (-) acyl carrier protein
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  • H1D33_25775
  • QLQ36638.1
 5706332 - 5707813 (-) AMP-binding protein
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  • H1D33_25780
  • QLQ36639.1
 5707843 - 5708619 (-) cyclase family protein
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Polyketide-specific information
Subclass Anthracycline
Starter unit Malonyl-CoA
Ketide length 10
Cyclic? yes
Polyketide-synthases
Genes Properties Modules
H1D33_25780
+
H1D33_25715
+
H1D33_25700
+
H1D33_25685
+
H1D33_25690
+
H1D33_25695
+
H1D33_25640 		Synthase subclass: Type II
NRP-specific information
Subclass N/A
Cyclic? yes
Saccharide-specific information
Subclass hybrid/tailoring
Sugar-encoding genes
  • rfbA, rfbB, H1D33_25635, H1D33_25630, H1D33_25620, H1D33_25620, H1D33_25625, H1D33_25620, H1D33_25615, H1D33_25610, H1D33_25595
Glycosyltransferases
H1D33_25625
Specificity: NDP-4-keto-L-digitoxose
Evidence:
  • Sequence-based prediction
Annotation changelog
MIBiG version Submitter Notes
3.0
  • Hidden contributor (ID: 4GMNAHXMUXC5VUH4UYMKD6EG, no GDPR consent given).
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Submitted
  • Added version to NCBI accession
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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