LOCUS       BGC0002103              8695 bp    DNA     linear   CON 12-FEB-2021
DEFINITION  Bacillus subtilis subsp. subtilis str. 168 complete genome.
ACCESSION   BGC0002103
VERSION     BGC0002103
KEYWORDS    .
SOURCE      Bacillus subtilis subsp. subtilis str. 168
  ORGANISM  Bacillus subtilis subsp. subtilis str. 168
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to AL009126.
            On Aug 12, 2009 this sequence version replaced NC_000964.2.
            On or before Mar 17, 2009 this sequence version replaced
            gi:32468687, gi:32468690, gi:32468695, gi:32468704, gi:32468715,
            gi:32468723, gi:32468738, gi:32468743, gi:32468745, gi:32468758,
            gi:32468767, gi:32468778, gi:32468787, gi:32468796, gi:32468802,
            gi:32468809, gi:32468813, gi:32468825, gi:32468830, gi:32468833,
            gi:32468840, gi:38680335.
            Annotation results relative to BLAST similarities, COG
            assignations, enzymatic function prediction (PRIAM software), TMHMM
            and SignalP predictions, and synteny conservation (Syntonizer
            software) are available in the MicroScope annotation platform
            http://www.genoscope.cns.fr/agc/microscope.
            The large cluster comprising the i, h and g rDNA regions could not
            be properly resolved, we therefore incorporated the original
            sequence of rRNAihg in the final assembly.
            COMPLETENESS: full length.
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 16:55:48
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 8695
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..3870)
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:BSU35040"
                     /db_xref="EnsemblGenomes-Tr:CAB15509"
                     /db_xref="GOA:O34986"
                     /db_xref="InterPro:IPR004843"
                     /db_xref="InterPro:IPR011401"
                     /db_xref="InterPro:IPR013783"
                     /db_xref="InterPro:IPR029052"
                     /db_xref="InterPro:IPR036415"
                     /db_xref="SubtiList:BG14123"
                     /db_xref="UniProtKB/Swiss-Prot:O34986"
                     /db_xref="GeneID:936623"
                     /gene="yvnB"
                     /inference="ab initio prediction:AMIGene:2.0"
                     /locus_tag="BSU_35040"
                     /note="Evidence 3: Putative function from multiple
                     computational evidences; PubMedId: 27542896; Product type
                     e: enzyme"
                     /old_locus_tag="BSU35040"
                     /product="putative exported hydrolase"
                     /protein_id="NP_391384.1"
                     /transl_table=11
                     /translation="MRKYTVIASILLSFLSVLSGGHHESKAFPVVQQELFQTPHYIPLL
                     ENPPQIDVKAEMRSNLMIEAQVRDNSYQAFSAFLFYKQSNELGYKMVPMDPTPGAVRKF
                     LAQIPKRLIWNSELEYYIVMSNGKQRVESETKKLKLEGYQADLAHIPELLITELAVDTK
                     NIGRADGYEFIEIFNTTDRTIDFKDYHIRYRYPKEGPDSDLIWRPDERILIPSGETFVV
                     WLKPAGHPELTSADFNRYYQTQLKEGKNLAVIDETEGMANTRPRAVVISTNTGKDISVA
                     HYRKHALRRLSSVLYKYPLNGTAELLNISIGEKNPSPGTVLQAQVPDQKRKIKLDKEKP
                     VIEDLTDRKPVRPAESIELRADIRDRSLVKTVAFYYRTDENKPFKRILAEKDRNDNLFH
                     YIVYSPELIGKDQLEYYVAAGDGINEARTPVKMIDIKQTSKAHGLRMNIENRDTLSGTQ
                     FLKATTEGRADSIKLWIDGKKQVTEPAMEKEVYFAFDTRKTNLYFKNAVTMEGKVLKVF
                     DDTTNKYRTYSVPLPETLLRKGKQLQRITIRSGSKVSPFDTAENRDDFLVKNARLVLSD
                     GTVIRDQRVSPEKELFIGDNQRSNKSWQFQFNLPDGLFTSQLLEWDTSKLSEGAHHIQA
                     SDGKENVSLVVRVDNSGPHIEPNITEGQTYKGNLILQADMYDKWSRIEEAEASLDGESI
                     TLPYHTSSSDLLPGKHSLKVTATDLAGNKTVIERIFKTEREHPDQPEVIDSEADTHKAK
                     LSVRVKDPTNDAMDVGFYRGFQYTARNHVKIFKHASLTEPPKSFVPERETPFTNKELER
                     VSAADGKTVSTENKELFPYHRFEVTVDPSIDENDLAESVWKGSSLPGRKVTMYAWNYRT
                     NEWQPVDSFVAKDDKPFTLKASVIAADFVRESKMNVIVQDEIPPAKDMYTFVWMSDTQY
                     YAESYPHIFDKQTEWIKDNQKQLNIKYVFHTGDIVDDSADIRQWKNADRSMSVLDKSGI
                     PYGVLAGNHDVGHKDGSYRAFGKYFGSDRFDKKFHYGGSYKNNRGHYDLISSNGNDYIM
                     LYMGWGITDEDIAWMNQVLKKHPDRMAILAFHEYLLVSGNRSPIGEKIFKEIVKPNPNV
                     VMVLSGHYHSAMRKTDELDDDGDGKPDRLVHQMLADYQGGPEGGQGYLRLLQFDQANDM
                     VHVSTYSPYVKDKNYYDTDTYGNKDEFSLSLDLKPRIKKVETDYFECNVYTNEELGKRE
                     QVKSGDTAEFRWDHLEPQSVYYWYIIVEDSFNGKTKSPIWKFKTKKETYRPAPDQFDFR
                     HVSNP"
     protocluster    1..8695
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[5781:6528]"
                     /cutoff="5000"
                     /detection_rule="(CDPS or cycdipepsynth)"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5782..6528
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(CDPS or cycdipepsynth)"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     cand_cluster    1..8695
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(CDPS or cycdipepsynth)"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..8695
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002103"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..8695
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="CDPS"
                     /region_number="1"
                     /rules="(CDPS or cycdipepsynth)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1..3870)
                     /db_xref="GeneID:936623"
                     /gene="yvnB"
                     /locus_tag="BSU_35040"
                     /old_locus_tag="BSU35040"
     gene            4035..4508
                     /db_xref="GeneID:936639"
                     /gene="yvnA"
                     /locus_tag="BSU_35050"
                     /old_locus_tag="BSU35050"
     CDS             4035..4508
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:BSU35050"
                     /db_xref="EnsemblGenomes-Tr:CAB15510"
                     /db_xref="GOA:O34692"
                     /db_xref="InterPro:IPR000835"
                     /db_xref="InterPro:IPR036388"
                     /db_xref="InterPro:IPR036390"
                     /db_xref="SubtiList:BG14122"
                     /db_xref="UniProtKB/Swiss-Prot:O34692"
                     /db_xref="GeneID:936639"
                     /function="16.3: Control"
                     /gene="yvnA"
                     /inference="ab initio prediction:AMIGene:2.0"
                     /locus_tag="BSU_35050"
                     /note="Evidence 3: Putative function from multiple
                     computational evidences; Product type r: regulator"
                     /old_locus_tag="BSU35050"
                     /product="putative transcriptional regulator"
                     /protein_id="NP_391385.1"
                     /transl_table=11
                     /translation="MDNSIHDELFQAIQQFALKRDKRVWQKVQIPSIGISSQHHDHLKK
                     DWTLTQLHIVSCIHTSQNVNNSFLASRLHISKAAVSKAVHALLKHNIITVTKKPGNKKE
                     IFYTLTDSGRKLAALHEQLHEKAKEQYKQLFNEFSIDDLKTVTAFFNLWIKYM"
     gene            complement(4549..5766)
                     /db_xref="GeneID:936624"
                     /gene="cypX"
                     /locus_tag="BSU_35060"
                     /old_locus_tag="BSU35060"
     CDS             complement(4549..5766)
                     /EC_number="1.14.-.-"
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:BSU35060"
                     /db_xref="EnsemblGenomes-Tr:CAB15511"
                     /db_xref="GOA:O34926"
                     /db_xref="InterPro:IPR001128"
                     /db_xref="InterPro:IPR002397"
                     /db_xref="InterPro:IPR017972"
                     /db_xref="InterPro:IPR030904"
                     /db_xref="InterPro:IPR036396"
                     /db_xref="PDB:3NC3"
                     /db_xref="PDB:3NC5"
                     /db_xref="PDB:3NC6"
                     /db_xref="PDB:3NC7"
                     /db_xref="SubtiList:BG12580"
                     /db_xref="UniProtKB/Swiss-Prot:O34926"
                     /db_xref="GeneID:936624"
                     /experiment="publication(s) with functional evidences,
                     PMID:4204912, 20690619, 27542896"
                     /function="16.5: Explore"
                     /function="16.14: Store"
                     /function="16.1: Circulate"
                     /gene="cypX"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007:cytochrome P450 (Score: 284; E-value: 3e-86)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="BSU_35060"
                     /note="Evidence 1a: Function from experimental evidences in
                     the studied strain; PubMedId: 4204912, 20690619, 27542896;
                     Product type e: enzyme"
                     /old_locus_tag="BSU35060"
                     /product="cyclo-L-leucyl-L-leucyl dipeptide oxidase,
                     pulcheriminic synthase"
                     /protein_id="NP_391386.1"
                     /sec_met_domain="p450 (E-value: 7.7e-39, bitscore: 122.3,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQSIKLFSVLSDQFQNNPYAYFSQLREEDPVHYEESIDSYFISR
                     YHDVRYILQHPDIFTTKSLVERAEPVMRGPVLAQMHGKEHSAKRRIVVRSFIGDALDHL
                     SPLIKQNAENLLAPYLERGKSDLVNDFGKTFAVCVTMDMLGLDKRDHEKISEWHSGVAD
                     FITSISQSPEARAHSLWCSEQLSQYLMPVIKERRVNPGSDLISILCTSEYEGMALSDKD
                     ILALILNVLLAATEPADKTLALMIYHLLNNPEQMNDVLADRSLVPRAIAETLRYKPPVQ
                     LIPRQLSQDTVVGGMEIKKDTIVFCMIGAANRDPEAFEQPDVFNIHREDLGIKSAFSGA
                     ARHLAFGSGIHNCVGAAFAKNEIEIVANIVLDKMRNIRLEEDFCYAESGLYTRGPVSLL
                     VAFDGA"
     gene            complement(5782..6528)
                     /db_xref="GeneID:936633"
                     /gene="pchC"
                     /locus_tag="BSU_35070"
                     /old_locus_tag="BSU35070"
     CDS             complement(5782..6528)
                     /EC_number="6.-.-.-"
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:BSU35070"
                     /db_xref="EnsemblGenomes-Tr:CAB15512"
                     /db_xref="GOA:O34351"
                     /db_xref="InterPro:IPR030903"
                     /db_xref="SubtiList:BG14121"
                     /db_xref="UniProtKB/Swiss-Prot:O34351"
                     /db_xref="GeneID:936633"
                     /experiment="publication(s) with functional evidences,
                     PMID:4204912, 19430487, 27542896"
                     /function="16.14: Store"
                     /function="16.1: Circulate"
                     /function="16.5: Explore"
                     /gene="pchC"
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     cycdipepsynth"
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /locus_tag="BSU_35070"
                     /note="Evidence 1a: Function from experimental evidences in
                     the studied strain; PubMedId: 4204912, 19430487, 27542896;
                     Product type e: enzyme"
                     /old_locus_tag="BSU35070"
                     /product="cyclodipeptide synthase (tRNA-dependent),
                     pulcherimminic acid precursor"
                     /protein_id="NP_391387.1"
                     /sec_met_domain="cycdipepsynth (E-value: 1.9e-99, bitscore:
                     320.4, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="CDPS (E-value: 1.7e-85, bitscore: 274.8,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGMVTERRSVHFIAEALTENCREIFERRRHVLVGISPFNSRFSE
                     DYIYRLIGWAKAQFKSVSVLLAGHEAANLLEALGTPRGKAERKVRKEVSRNRRFAERAL
                     VAHGGDPKAIHTFSDFIDNKAYQLLRQEVEHAFFEQPHFRHACLDMSREAIIGRARGVS
                     LMMEEVSEDMLNLAVEYVIAELPFFIGAPDILEVEETLLAYHRPWKLGEKISNHEFSIC
                     MRPNQGYLIVQEMAQMLSEKRITSEG"
     gene            6954..7463
                     /db_xref="GeneID:936625"
                     /gene="pchR"
                     /locus_tag="BSU_35080"
                     /old_locus_tag="BSU35080"
     CDS             6954..7463
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:BSU35080"
                     /db_xref="EnsemblGenomes-Tr:CAB15513"
                     /db_xref="GOA:P40762"
                     /db_xref="InterPro:IPR000835"
                     /db_xref="InterPro:IPR023187"
                     /db_xref="InterPro:IPR036388"
                     /db_xref="InterPro:IPR036390"
                     /db_xref="SubtiList:BG11018"
                     /db_xref="UniProtKB/Swiss-Prot:P40762"
                     /db_xref="GeneID:936625"
                     /experiment="publication(s) with functional evidences,
                     PMID:27542896"
                     /function="16.3: Control"
                     /gene="pchR"
                     /locus_tag="BSU_35080"
                     /note="Evidence 1a: Function from experimental evidences in
                     the studied strain; PubMedId: 27542896; Product type r:
                     regulator"
                     /old_locus_tag="BSU35080"
                     /product="transcriptional regulator for pulcherriminic acid
                     synthesis"
                     /protein_id="NP_391388.1"
                     /transl_table=11
                     /translation="MSDLTKQMIYDIYVRLLHLNEQKANTSLQQFFKEAAEEDVAEIPK
                     NMTSIHVIDCIGQHEPINNAGIARKMNLSKANVTKISTKLIKEEFINSYQLTDNKKEVY
                     FKLTRKGRRIFDLHEKLHKKKELAFYQFLDSFSQEEQKAVLKFLEQLTSTLEAEQTDGT
                     PDKPVK"
     gene            7484..8695
                     /db_xref="GeneID:936643"
                     /gene="pchE"
                     /locus_tag="BSU_35090"
                     /old_locus_tag="BSU35090"
     CDS             7484..8695
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:BSU35090"
                     /db_xref="EnsemblGenomes-Tr:CAB15514"
                     /db_xref="GOA:O34307"
                     /db_xref="InterPro:IPR011701"
                     /db_xref="InterPro:IPR020846"
                     /db_xref="InterPro:IPR036259"
                     /db_xref="SubtiList:BG14120"
                     /db_xref="UniProtKB/Swiss-Prot:O34307"
                     /db_xref="GeneID:936643"
                     /experiment="publication(s) with functional evidences,
                     PMID:15849754, 16850406, 27542896"
                     /function="16.1: Circulate"
                     /gene="pchE"
                     /gene_functions="transport (smcogs) SMCOG1106:major
                     facilitator transporter (Score: 148.8; E-value: 3e-45)"
                     /gene_kind="transport"
                     /locus_tag="BSU_35090"
                     /note="Evidence 1a: Function from experimental evidences in
                     the studied strain; PubMedId: 15849754, 16850406, 27542896;
                     Product type t: transporter"
                     /old_locus_tag="BSU35090"
                     /product="pulcherriminic acid efflux transporter"
                     /protein_id="NP_391389.1"
                     /transl_table=11
                     /translation="MAHTKSKAVLILYTVCFSAFFASLSQNIYSPILPIIKESFHVSTA
                     MVNLSVSVFMIVTAIMQIILGAIIDFKGARIVLITGILATAAASIGCAVTTDFTLFLIF
                     RMIQAAGSAALPLIAATTIGQLFTGNERGSAMGTYQMLLSVAPAIAPVLGGFIGGAAGY
                     EGIFWILAAISIVLLVTNSITFPKDSPTESMQQAKGNVFAHYKSIFTNRTGNVILTLSF
                     VLFFIYFAVIVYLPILLTEHYHIDVGIAGLLYLPLALSTIAGTFLFKRIQAKIGLHTLF
                     IGSNVIAACSIILFAVTHSVSLVLMALTLALFGISMGVIPPLYSTMITNEFEHNRGSAI
                     GMFNFIRYTGMAAGPMVSAYLLTMMPSAMSFSLLGLGFAALSFCLLPPMFSPQKRTKQK
                     KHHM"
ORIGIN
        1 ttaaggattg gagacatggc gaaaatcaaa ctggtctggt gccggacgat atgtttcttt
       61 tttcgtcttg aacttccaga tcggtgattt cgttttgccg ttaaaactgt cttccactat
      121 aatgtaccaa taataaacag actgcggttc aagatgatcc cagcggaatt cagctgtgtc
      181 cccgctcttc acttgctccc gttttccgag ctcttcattg gtatatacgt tacattcaaa
      241 ataatcggtt tccacctttt tgattcttgg cttcaaatca agcgaaaggg aaaattcatc
      301 cttgttgcca taggtatccg tatcatagta gttcttatcc tttacatacg gagagtacgt
      361 cgatacgtgc accatgtcat tggcttggtc aaattgaagc aaacgcaaat acccttggcc
      421 gccttctggc ccgccctgat aatcggccag catttgatgc acaagacgat ccggtttccc
      481 atcaccatca tcatccagct catctgtttt tctcatcgca ctatgataat gcccgctcaa
      541 caccatcacg acattcgggt tcggctttac gatttctttg aatattttct ctccgattgg
      601 gcttctgttt ccgctgacca gcaaatactc atgaaaagct aaaatggcca tccggtcagg
      661 atgcttcttc agcacctgat tcatccacgc gatgtcttca tccgtaatgc cccatcccat
      721 gtacagcatg atataatcat tgccgttgct agaaatcagg tcataatgtc cgcggttatt
      781 tttataggaa ccgccataat gaaatttttt atcaaaacga tcgctgccaa aatatttgcc
      841 aaacgccctg taggagccgt ccttatgtcc gacatcgtga ttgccggcca gcacgccgta
      901 aggaattccg ctcttatcca gaacggacat tgaccgatca gcgtttttcc actgtctgat
      961 atcggcggaa tcatctacaa tatcacccgt gtggaacaca tatttgatat tgagctgctt
     1021 ttgattatct tttatccatt cggtctgttt atcaaaaata tgcggatagc tttcggcgta
     1081 atactgtgta tcagacatcc agacaaatgt gtacatatcc ttcgcaggag gaatttcatc
     1141 ctgtacaatc acattcattt tcgactcccg tacaaaatcg gcggcaatca cagacgcttt
     1201 cagcgtaaac ggtttgtcat ccttcgcgac gaagctgtca acaggctgcc attcgtttgt
     1261 ccgataattc caagcataca tcgtcacctt tctccccggc aaagaactgc ccttccagac
     1321 tgattctgcc agatcattct catcaattga tggatcaacc gtcacttcaa agcgatgata
     1381 agggaaaagc tctttgtttt ctgtcgatac tgtttttcca tcagcagctg atacccgctc
     1441 caattcttta ttcgtaaatg gggtctccct ttccggcaca aagcttttcg gcggttcagt
     1501 cagtgacgcg tgcttaaata ttttcacatg attgcgcgct gtatattgaa atcccctata
     1561 aaaaccaaca tccatggcat cattcgtcgg atctttcacc cgtacagaaa gcttggcctt
     1621 atgcgtgtcg gcttcgctgt ctataacctc cggctggtcc ggatgctctc gttcggtttt
     1681 aaatatcctc tcaatcactg ttttattgcc cgcgagatcc gttgctgtca cttttagaga
     1741 atgttttccc ggcaaaaggt cagaagatga cgtatgatat gggagtgtaa tgctttctcc
     1801 atctaacgac gcctccgctt cctcaattct gctccattta tcatacatat ccgcctgcag
     1861 gataaggttt cctttatacg tttgcccctc ggtgatattc ggctcaatgt gaggtcctga
     1921 attatctacc cgtacaacca ggctgacatt ttccttcccg tcgcttgctt gaatatggtg
     1981 agcgccttca gaaagctttg atgtatccca ctccagcagc tgtgatgtaa atagtccgtc
     2041 aggcagattg aactgaaatt gccagctttt attcgagcgc tgattatcgc caataaagag
     2101 ttccttctca ggagacaccc tctgatctct gatcaccgtt ccatccgata acaccaatcg
     2161 ggcgtttttg actaagaagt catcacggtt ttcagccgta tcaaacggag acaccttcga
     2221 acctgaacgt atcgtaatac gctgcagctg cttccctttc cgaagcagtg tctctggcaa
     2281 tggaacagaa taggtgcggt atttatttgt tgtatcatcg aacaccttca gcactttgcc
     2341 ttccatcgtt accgcgtttt taaaataaag gttcgttttc ctcgtatcga atgcaaaata
     2401 tacttctttt tccatcgcgg gctctgtcac ttgttttttc ccgtcaatcc atagcttaat
     2461 gctgtcagct cgcccttctg tcgtggcttt gagaaattga gttcccgaca gcgtgtctct
     2521 gttttcaata ttcatccgca gaccgtgcgc cttgcttgtt tgtttaatat caatcatttt
     2581 gaccggcgtt ctcgcttcat tgatcccgtc cccggccgcc acataatact ctagctgatc
     2641 cttcccgatc aattcaggcg aatacacaat ataatggaag agattatcgt tgcggtcttt
     2701 ttcagcaaga attcgcttaa acggtttgtt ctcatccgtc cggtagtaaa aggccactgt
     2761 tttcactagg cttcggtccc gtatgtcagc ccgcagctca atgctttcag cagggcggac
     2821 aggcttgcgg tcagtcaaat cctcaatgac cggcttttct ttatcgagct tgattttccg
     2881 tttctggtcg ggcacctgtg cctgtaatac ggttccggga gacggattct tttccccgat
     2941 tgaaatgttc agaagttctg ctgttccgtt cagtggatac ttataaagca ctgatgacag
     3001 ccggcgcagc gcatgtttcc gataatgcgc aaccgaaatg tcctttcctg tattcgttga
     3061 tatcacaacg gcccgcggcc tcgtattggc cataccctct gtctcgtcta tcaccgcaag
     3121 attttttcct tcttttagtt gtgtctgata ataacgattg aaatccgctg acgtcaattc
     3181 tggatgcccg gcaggcttca gccagacgac aaacgtttcg ccggatggta tcaagatcct
     3241 ttcatcaggt ctccaaatca gatcggagtc aggcccttct tttgggtagc ggtaacggat
     3301 atggtagtct ttaaaatcaa tcgttctgtc agtcgtatta aaaatctcaa taaattcata
     3361 accgtctgcg cgtcctatat tcttcgtatc aaccgccagc tctgtgatca gaagctccgg
     3421 aatatgggca aggtcagcct ggtatccttc aagcttcagt ttcttcgtct ccgactcaac
     3481 ccgctgcttc ccattcgaca tcacaatgta atactcaagc tcgctgttcc aaatcaaacg
     3541 tttcggaatt tgtgccaaaa acttgcggac cgctccgggg gtcgggtcca tcggcaccat
     3601 tttgtagccc agctcattac tttgtttata aaaaagaaag gcggaaaatg cttgatacga
     3661 attatcgcga acttgagcct caatcattag attgcttctc atttcagctt tcacatcaat
     3721 ttgaggagga ttttcaagca agggtatgta atgaggcgtt tgaaatagtt cctgctgtac
     3781 aacaggaaaa gctttcgatt catgatggcc gccagacaaa acggaaagaa aagacagtaa
     3841 tatagaagca ataaccgtgt atttcctcat caaagcacct cccttttcat gtattttgcg
     3901 tctgaacagg gaagcttatg agttcatttt tgtgcataaa gaacaaaaaa acgtctgtct
     3961 cttgaaaatt ccactattac ccagtaaact aaggatagtt aaccagttaa caattaaatc
     4021 agagaggcaa attgatggac aacagcattc atgatgaatt atttcaagct atacaacaat
     4081 tcgcattaaa aagggataaa cgcgtctggc aaaaggtgca gataccatcc attggcatat
     4141 cctcacaaca tcacgatcac ctcaaaaaag actggacttt gacccagctt cacattgttt
     4201 cctgcataca taccagccaa aatgtgaaca acagcttctt agcatcccgt ttacacatct
     4261 caaaagccgc tgtttcaaaa gcggttcatg ctttgttgaa acacaatatc attacagtga
     4321 caaaaaagcc tggcaacaaa aaagaaattt tttatacgct aactgatagc ggcagaaaat
     4381 tggcggcatt acatgaacag cttcatgaga aagcaaagga gcaatataaa cagcttttca
     4441 atgaattttc aattgatgat ttaaaaacag tgactgcgtt tttcaacttg tggatcaaat
     4501 atatgtaaaa agagtctcac ccgcatggga gagacctttg gaattctatt atgccccgtc
     4561 aaacgcaacg agaagtgaaa caggtccgcg tgtatacaga ccggactcag cataacaaaa
     4621 atcttcctct aatctgatat tccgcatctt atccagcaca atattagcta caatttcgat
     4681 ttcgtttttg gcaaaagctg ctcctacaca gttatgaatg ccggatccga aagcgagatg
     4741 ccgggcggcg ccgctaaaag cgctcttgat accaagatct tcccgatgaa tattaaacac
     4801 gtcaggctgt tcaaatgctt cagggtcccg gttagccgca ccgatcatac aaaaaacaat
     4861 cgtatctttt ttgatttcca taccgccgac cactgtatct tgggacagct gccgcggaat
     4921 cagctgaacc ggcggtttat aacgcaatgt ctccgcaatg gctctcggaa ctaacgaacg
     4981 gtcagccaaa acatcattca tctgctcagg attgttgagc aaatggtaga tcatcagtgc
     5041 cagcgtctta tcagccggtt ccgttgcggc taacagcaca ttaagaatca gtgcgagtat
     5101 atccttgtcc gacagcgcca tgccttcata ttcagaagta cataggatcg aaattaaatc
     5161 tgatcccgga ttgacgcgac gttctttaat gaccggcatc aagtattggg aaagctgttc
     5221 gctgcaccat aacgaatgtg cccgcgcttc aggagattga gagatactcg tgataaaatc
     5281 ggcaactccg ctgtgccact cagagatttt ttcatggtct cttttatcca gcccgagcat
     5341 gtccatcgtg acgcacaccg caaacgtctt tccaaaatca ttgacgagat cacttttccc
     5401 tctttcaaga taaggcgcta acaagttttc tgcattttgt ttaatcaatg gagacagatg
     5461 atccagtgcg tcaccgataa agcttctcac tacaattctt cttttggcag agtgttcttt
     5521 tccatgcatt tgggccagca cagggcctcg catgactggt tcggcacgct caacaagtga
     5581 tttcgtcgtg aagatatccg gatgctgaag gatatagcgg acatcatgat agcggctgat
     5641 aaaataactg tctatcgact cttcataatg aaccggatct tcctcccgca gttgtgaaaa
     5701 ataagcatat ggattgtttt gaaattgatc agaaagcaca ctaaacaatt taatcgattg
     5761 gctcatgttt tactccccct atcatccttc agatgtgatc cgtttctcag aaagcatctg
     5821 cgccatttcc tgtacaatga gatacccttg attcggccgc atacaaatag aaaattcatg
     5881 gttactgatc ttctcaccca gcttccacgg acgatgataa gcaaggagtg tctcttccac
     5941 ctctaaaata tccggagctc cgataaaaaa cggcagctca gctatgacat attccacagc
     6001 caaattcagc atatcctcac tgacttcttc catcatcaaa ctgacgcccc gcgcacgccc
     6061 gattatcgct tcacgagaca tgtccaaaca agcatgtcga aaatgaggct gctcaaaaaa
     6121 tgcatgttca acttcttgtc tcaacagctg gtaggctttg ttatctataa aatcagaaaa
     6181 tgtatgaatc gccttcggat ccccgccatg agccacaagg gctctttctg caaatctcct
     6241 gtttcgtgat acctctttcc ttacttttcg ttcagccttt cctctcggag ttccaagcgc
     6301 ttctagaaga ttagccgcct catgccctgc aagtaaaact gaaacgcttt taaattgagc
     6361 tttcgcccat ccaattaatc tgtaaatata atcctctgaa aacctgctgt taaatgggct
     6421 gatccccacc aaaacatgcc tgcgccgttc aaatatttct ctgcagtttt ctgttaatgc
     6481 ctcagcaata aaatgcacag accttctttc cgttaccatt ccggtcatct cattcacccc
     6541 taaaacttat cccgctttaa gtcatcaccc tcaaatacaa gatggaatag ctagaacaac
     6601 tgaacaagac gaatggcagg catcacttaa agtttccaaa tgttttttaa gattttaata
     6661 atcattttca atacccatat catgataaat gtttactagt aaacattagt cattttatcc
     6721 cttttccttc caaacgtcaa tatgatctgt gcccaaatat ttgaaaataa atgtaaacat
     6781 cacattttcc acacactttt tacacgcata ttcagcatga catatcaaca aatagtctaa
     6841 acaccaacaa ttgacaacga aaacaagaaa agataaaata aaattgttca ccagtaaacg
     6901 atttgtttta ttgtttcatc gtaaactatt attcctaaga aagaaggtag cctatgtctg
     6961 atttgacaaa acagatgata tacgacatat acgtgagact gctgcacctt aatgaacaaa
     7021 aagcgaacac ttcacttcag caatttttta aggaggccgc agaagaggat gtagctgaaa
     7081 ttcccaaaaa tatgacaagc attcacgtca ttgactgcat cggccagcat gaacccatta
     7141 ataatgccgg aattgccaga aaaatgaact tatcgaaagc gaatgtaacg aaaatcagca
     7201 caaaactgat caaggaagaa ttcattaaca gctatcagct gacagataac aaaaaagaag
     7261 tttattttaa attaacccgt aaaggcagac ggattttcga cttacatgag aaactgcata
     7321 aaaaaaagga gctggctttt taccaattcc tcgattcatt ttcacaagaa gaacaaaagg
     7381 ctgtattgaa gtttctagag cagttgacgt caacacttga agcagaacaa accgatggga
     7441 ctccagacaa acctgtaaag taaacaaaaa ggcggtgtac actttggctc atacaaaatc
     7501 aaaggcagta ttgatcttat acactgtttg cttcagtgca ttttttgcat ctttaagcca
     7561 gaacatttat tcacctattc ttccgatcat taaagaatca ttccatgttt ccacagctat
     7621 ggtgaacctg tcagtctcag tttttatgat tgtgacagca ataatgcaaa ttatattagg
     7681 agcgatcatt gattttaaag gcgctcggat cgtcttgatt accggtattc tggcaacggc
     7741 agcagccagc atcggctgtg cggtgactac tgactttacc ttgtttctga tattcagaat
     7801 gatacaggca gccggttccg cagcactgcc tcttattgct gccacaacga tcggacagct
     7861 gtttacagga aatgaacgcg ggagtgcaat gggaacgtat caaatgctcc tgtctgtcgc
     7921 accggctatt gctccagttc taggaggatt cataggcgga gcagccggat acgaagggat
     7981 tttttggata cttgcggcca tctctatcgt tttgctggtg acaaacagca tcacctttcc
     8041 taaagattct ccaactgaat ctatgcagca agccaaaggc aatgtgttcg ctcattataa
     8101 atcaatattt acaaatcgaa cagggaacgt cattttgact ttaagttttg ttctcttttt
     8161 catttatttt gcagtaattg tctacctccc aatattgctg acagagcatt accatataga
     8221 tgtgggtata gcaggactgt tatatttgcc gctggcgctg agcacgattg caggtacgtt
     8281 tctgtttaaa agaatacagg caaaaatcgg gctgcacacc ttgtttatcg gaagcaatgt
     8341 gattgccgcc tgcagcatca ttttatttgc tgttacacat tccgtttctc tcgttctcat
     8401 ggctctgacg ctggcactgt ttggcatctc gatgggggtt attcctccct tgtactctac
     8461 aatgattact aatgaatttg agcacaacag agggagtgca atcggaatgt ttaactttat
     8521 ccgatataca ggcatggcag caggtccgat ggtatctgcc tacttgctca caatgatgcc
     8581 gtctgccatg tcctttagcc tcctaggcct tggatttgcc gcattgagct tttgccttct
     8641 tccgccaatg ttttcgccgc agaagcgcac gaaacaaaaa aagcaccaca tgtaa
//
