BGC0002104 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002104 (Streptomyces eurocidicus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS-like,T1PKS	1	125,286	High	eurocidin D/eurocidin E	NRPS:Type I+PKS:Type I

BGC0002104 - Region 1 - NRPS-like,T1PKS

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Location: 1 - 125,286 nt. (total: 125,286 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 125,287
Label		BGC0002104

Tailoring functions

Oxidoreductases: oxygenase

AF335_RS14780: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

AF335_RS14805: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: aminotransferase glycosyltransferase

AF335_RS14785: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

AF335_RS14790: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

Hydrolases: esterase

AF335_RS14705: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

AF335_RS14735: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

AF335_RS14700: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

AF335_RS14740: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

AF335_RS14745: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

AF335_RS14750: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

AF335_RS14755: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

AF335_RS14770: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002104.3	1.19	NRPS, PKS	eurocidin D, eurocidin E	Streptomyces eurocidicus ATCC 27428
BGC0001132.4	0.65	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.64	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.64	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.62	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.61	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.61	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	0.60	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001873.3	0.59	NRPS	pyrrolizixenamide A	Xenorhabdus szentirmaii DSM 16338
BGC0001825.4	0.58	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002104.3	1.00	NRPS, PKS	eurocidin D, eurocidin E	Streptomyces eurocidicus ATCC 27428
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001258.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 125286:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Thr) + (ccmal - ohmal - Me-ohmal - ohmal - ohmal - ohmal) + (redmal) + (ccmal - ccmal) + (ohmal - ccmal - ccmal - ccmal - ccmal) + (mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 41824:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Thr) + (ccmal - ohmal - Me-ohmal - ohmal - ohmal - ohmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 7908 - 125286:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Thr) + (ccmal - ohmal - Me-ohmal - ohmal - ohmal - ohmal) + (redmal) + (ccmal - ccmal) + (ohmal - ccmal - ccmal - ccmal - ccmal) + (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

AF335_RS14700: Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (29..420): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNVGMVHK
Nearest Stachelhaus code(s):: Thr DFWNVGMVHK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

AF335_RS14740: mal - mal - mmal - mal - mal - mal

PKS_AT (567..867): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 218.4
inactive: 91.9
Methoxymalonyl-CoA: 56.7
Methylmalonyl-CoA: 47.2
Propionyl-CoA: 29.7
Acetyl-CoA: 24.5
Ethylmalonyl-CoA: 18.9
Isobutyryl-CoA: 18.8
Benzoyl-CoA: 18.7
2-Methylbutyryl-CoA: 11.9
CHC-CoA: 9.6
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2324..2623): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 202.0
inactive: 83.9
Methoxymalonyl-CoA: 75.8
Methylmalonyl-CoA: 63.0
Propionyl-CoA: 37.1
Isobutyryl-CoA: 30.2
Acetyl-CoA: 28.0
2-Methylbutyryl-CoA: 25.2
Ethylmalonyl-CoA: 25.0
CHC-CoA: 22.5
Benzoyl-CoA: 20.6
fatty_acid: 15.7
trans-1,2-CPDA: 12.8
3-Methylbutyryl-CoA: 0.0

PKS_AT (3860..4158): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 214.6
Ethylmalonyl-CoA: 206.0
Methoxymalonyl-CoA: 167.5
trans-1,2-CPDA: 138.9
2-Methylbutyryl-CoA: 138.9
Propionyl-CoA: 106.4
CHC-CoA: 105.6
Malonyl-CoA: 96.8
Isobutyryl-CoA: 76.6
Benzoyl-CoA: 63.3
Acetyl-CoA: 45.2
fatty_acid: 42.7
3-Methylbutyryl-CoA: 39.3
inactive: 25.0

PKS_AT (5396..5696): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 209.8
inactive: 99.8
Methoxymalonyl-CoA: 73.2
Methylmalonyl-CoA: 67.5
Isobutyryl-CoA: 34.3
Propionyl-CoA: 32.1
Ethylmalonyl-CoA: 31.4
Acetyl-CoA: 21.8
trans-1,2-CPDA: 18.0
fatty_acid: 18.0
2-Methylbutyryl-CoA: 15.5
Benzoyl-CoA: 15.0
CHC-CoA: 12.0
3-Methylbutyryl-CoA: 3.5

PKS_AT (6919..7206): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 237.7
inactive: 120.0
Methoxymalonyl-CoA: 69.7
Methylmalonyl-CoA: 65.9
Propionyl-CoA: 38.2
Isobutyryl-CoA: 30.7
Ethylmalonyl-CoA: 26.7
Acetyl-CoA: 22.2
2-Methylbutyryl-CoA: 21.3
Benzoyl-CoA: 18.5
fatty_acid: 14.1
CHC-CoA: 13.5
trans-1,2-CPDA: 12.4
3-Methylbutyryl-CoA: 0.0

PKS_AT (8432..8724): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 221.5
inactive: 108.0
Methoxymalonyl-CoA: 60.2
Methylmalonyl-CoA: 56.9
Propionyl-CoA: 34.7
Isobutyryl-CoA: 30.8
Ethylmalonyl-CoA: 22.9
Benzoyl-CoA: 20.1
2-Methylbutyryl-CoA: 18.1
Acetyl-CoA: 16.1
trans-1,2-CPDA: 8.6
CHC-CoA: 8.4
fatty_acid: 8.3
3-Methylbutyryl-CoA: 0.0

AF335_RS14745: mal

PKS_AT (551..840): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 235.8
inactive: 101.4
Methoxymalonyl-CoA: 70.6
Methylmalonyl-CoA: 64.3
Propionyl-CoA: 39.2
Acetyl-CoA: 25.5
Isobutyryl-CoA: 24.6
Ethylmalonyl-CoA: 22.7
Benzoyl-CoA: 22.3
2-Methylbutyryl-CoA: 21.6
CHC-CoA: 12.3
fatty_acid: 11.8
trans-1,2-CPDA: 10.3
3-Methylbutyryl-CoA: 0.0

AF335_RS14750: mal - mal

PKS_AT (547..833): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 241.8
inactive: 102.8
Methoxymalonyl-CoA: 69.3
Methylmalonyl-CoA: 59.5
Propionyl-CoA: 35.3
Acetyl-CoA: 24.2
Isobutyryl-CoA: 22.1
2-Methylbutyryl-CoA: 18.3
Benzoyl-CoA: 15.4
Ethylmalonyl-CoA: 15.3
CHC-CoA: 5.2
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2255..2542): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 240.2
inactive: 110.4
Methoxymalonyl-CoA: 68.9
Methylmalonyl-CoA: 59.0
Propionyl-CoA: 36.3
Isobutyryl-CoA: 20.3
Benzoyl-CoA: 17.9
Acetyl-CoA: 17.0
2-Methylbutyryl-CoA: 16.6
Ethylmalonyl-CoA: 15.1
fatty_acid: 9.2
CHC-CoA: 8.5
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

AF335_RS14755: mal - mal - mal - mal - mal

PKS_AT (565..864): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 206.7
inactive: 94.2
Methoxymalonyl-CoA: 58.8
Methylmalonyl-CoA: 55.9
Isobutyryl-CoA: 36.8
Propionyl-CoA: 33.4
Acetyl-CoA: 22.3
Benzoyl-CoA: 18.2
2-Methylbutyryl-CoA: 15.0
CHC-CoA: 6.6
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -3.3

PKS_AT (2098..2388): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 236.6
inactive: 117.3
Methoxymalonyl-CoA: 57.5
Methylmalonyl-CoA: 47.0
Acetyl-CoA: 20.9
Benzoyl-CoA: 18.7
Ethylmalonyl-CoA: 13.6
Propionyl-CoA: 12.6
Isobutyryl-CoA: 11.7
CHC-CoA: 6.8
2-Methylbutyryl-CoA: 1.7
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (3818..4108): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 234.2
inactive: 113.0
Methoxymalonyl-CoA: 57.4
Methylmalonyl-CoA: 53.2
Propionyl-CoA: 23.8
Acetyl-CoA: 21.7
Benzoyl-CoA: 20.2
Isobutyryl-CoA: 12.3
Ethylmalonyl-CoA: 11.5
CHC-CoA: 7.0
fatty_acid: 6.8
2-Methylbutyryl-CoA: 4.3
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (5519..5809): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 222.0
inactive: 112.9
Methoxymalonyl-CoA: 62.2
Methylmalonyl-CoA: 49.1
Propionyl-CoA: 21.4
Acetyl-CoA: 20.7
Benzoyl-CoA: 15.2
Ethylmalonyl-CoA: 14.3
Isobutyryl-CoA: 11.5
CHC-CoA: 10.1
fatty_acid: 7.7
2-Methylbutyryl-CoA: 4.3
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (7231..7522): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 91.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 235.2
inactive: 106.7
Methoxymalonyl-CoA: 52.1
Methylmalonyl-CoA: 46.4
Propionyl-CoA: 27.0
Acetyl-CoA: 22.8
Ethylmalonyl-CoA: 17.9
Benzoyl-CoA: 17.9
fatty_acid: 9.0
Isobutyryl-CoA: 8.6
CHC-CoA: 5.5
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -2.1

AF335_RS14770: mal

PKS_AT (579..880): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Methylmalonyl-CoA: 62.5%; Isobutyryl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 147.0
inactive: 77.2
Methoxymalonyl-CoA: 74.5
Methylmalonyl-CoA: 72.3
Ethylmalonyl-CoA: 44.0
Propionyl-CoA: 41.5
Acetyl-CoA: 24.4
2-Methylbutyryl-CoA: 24.3
fatty_acid: 17.0
CHC-CoA: 13.3
Isobutyryl-CoA: 13.0
trans-1,2-CPDA: 10.6
Benzoyl-CoA: 8.3
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor