LOCUS       BGC0002110             11040 bp    DNA     linear   BCT 24-JUN-2020
DEFINITION  Streptomyces rimosus subsp. rimosus strain WC-3908 birimositide gene
            cluster, complete sequence.
ACCESSION   BGC0002110
VERSION     BGC0002110
KEYWORDS    .
SOURCE      Streptomyces rimosus subsp. rimosus
  ORGANISM  Streptomyces rimosus subsp. rimosus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 16:56:54
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 11040
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            1..168
                     /gene="brtA1"
     CDS             1..168
                     /codon_start=1
                     /gene="brtA1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_ha"
                     /gene_kind="biosynthetic-additional"
                     /note="LanA, alpha peptide of birimositide"
                     /product="BrtA1"
                     /protein_id="QKV49893.1"
                     /sec_met_domain="mature_ha (E-value: 3.1e-13, bitscore:
                     38.4, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFFEGDVSLIEEIADQDFEGVAYGACTTNTFSLSNALGNNGGWCT
                     LTKECQPNCN"
     source          1..11040
                     /db_xref="taxon:132474"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces rimosus subsp. rimosus"
                     /strain="WC-3908"
                     /sub_species="rimosus"
     misc_feature    1..11040
                     /note="birimositide gene cluster"
     subregion       1..11040
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="ribosomal"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11040
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            219..377
                     /gene="brtA2"
     CDS             219..377
                     /codon_start=1
                     /gene="brtA2"
                     /note="LanA, beta peptide of birimositide"
                     /product="BrtA2"
                     /protein_id="QKV49894.1"
                     /transl_table=11
                     /translation="MEQQDFLGAYDELELIELSEEEWHGGTGPLCVAASVAASAAFCPT
                     TKCTSKC"
     gene            423..3461
                     /gene="brtM"
     CDS             423..3461
                     /codon_start=1
                     /gene="brtM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     656.5; E-value: 1.2e-198)"
                     /gene_kind="biosynthetic"
                     /note="LanM"
                     /product="BrtM"
                     /protein_id="QKV49892.1"
                     /sec_met_domain="DUF4135 (E-value: 3.9e-71, bitscore:
                     229.2, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 9e-51, bitscore:
                     161.7, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIPTPCRPGTLPHLCARSEVSLISPQSLYPEFDSAEIRETITPLS
                     AFDGAVLEILSKPALVKEAQKEELPEVAARWSAENEQYPFQRTVRALAVQVCRTGVVTR
                     HEALFSDCDEITRQLLSRVESRLKNACTRSLVAAINTASADGKLRGEHGEARYRHFLAN
                     CSAEQLGESFPLLLSAVGHLLRNTAAAFDELCTRLSADRQRIAETFGIVGSDALTSIGQ
                     ADGDAHAHGRSVSILTFASGARLVYKPRDISCEAAYEAIVAHVNSCAGTSLLAARTLDR
                     AGYGYVEYISAEDVSGVSPQFMRRCGELAAVFHLLDARDMHFENIVATRRGPVPVDLET
                     LMHPARVHVGPQPEAPDNAYDVIARSLYGIGILPLVMAGKGKTSGHVDLGFLGGDNHGP
                     SPFKGIAFENPFSDKIRVVLRAEETGQRSTVVSAADEENIHHLAEQMAAGFTRTVRALT
                     HQREAWTQLMTRLARRMRVRYVHNPTALYIQNLRLIASASAMAAPATALGLLKRIAIAS
                     KTSHRSIVVSELKQMAERDVPYFTAKATDTVLYDADGNDTGARLARTPLARVLEKAASI
                     DEAAITQQLSLLYSAFCSRFPDNHLTGAEVWPARPAVARSQGLLDVARSLADALVATSL
                     PDKFAHLPRTWIGPLASAQAERAWPCGVLGYDLYTGRTGPALALAAAGRVLGDDAYTDV
                     ARQIFTTSAEILVGRRYEQRSVDQSGPGAYTGLAGLLFALRTAGDLLQEPDWIRAAQDA
                     VPLVVEQVDGESAVDVISGVAGIAAAIDAIGGTHADNVLPSLTSMLCTMAERAHGSWTE
                     QSGFAHGVAGVLHALNVLRPRAGDRSLRQRVDRATGVLLDRLAAFYDPTEANWFSDITA
                     PGRFSTGWCHGAGGISLALLACVQHDGDERARRWLGQAITNTVEHGFGRNLTWCHGDLG
                     NHAVLSEAATKLGDDALAGQVRSIEDTGLRAEVLQDKLTDHRSRYAHTNSVMVGSSGVL
                     LHLLNRIDPGLRTSPLSLSCGAR"
     gene            3461..5617
                     /gene="brtT"
     CDS             3461..5617
                     /codon_start=1
                     /gene="brtT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 274.1; E-value:
                     4.1e-83)"
                     /gene_kind="transport"
                     /note="LanT with protease domain"
                     /product="BrtT"
                     /protein_id="QKV49895.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 5.3e-29, bitscore:
                     89.9, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKRVPTVTQVTQTECGLCCCISVMRYWGRGEDFFTVRQDLEAGR
                     DGLGAKQLADFLRSRGMETKAFRAKNIDALAHFTVPVILYWEEYHFLVLEKFDGRTATV
                     MDPAVGRRRLSRADLEAGFSEIVIVPEPGTGFTYQSLPATKDWRTVPLFAERAAARIAL
                     VAMLSLSSYLAALGIPMLTKWAVDRQAHWRDLGDLSLVIVVVVAAAAGYLLLWLLRVTA
                     LSALISILGRALMTHTFRKLLSLPYKFFTTRQPGELLFRLNTVNVVRDLLSSRIAQGIL
                     DIGTLVCISVYLFVSEWRIGLVAATMFALNAVYLWATRNRVKEVTDAEISQLGRSQSAQ
                     LDAIVSIPTIKMGGYAQQFADEWAKVYQSSLDAMRQRMRLQQGWIAGIATMTQMFGPAV
                     LLLTSLYFVSQGVVSLGAAIAIQSVSMTYFSMTTSVFQMFTEFTEASRYMARLSDITAY
                     TSEGAGGPERHLEEASVTLRDVRFQYTRHAAPVVDGVSMHIPAGSRVALVGASGSGKST
                     LGRLICGLHEPTGGAIEFAGRPMADYDRDFLRRQIGYIPQEVHLHNRTILENLTLGQDI
                     PPEKVREYCSQVGILDFVEALPMGLKTLVSELGANFSGGQRQRLAIVRTLLQQPRIIVM
                     DEATASLDTLNERRVTKIIEDTGATQVIIAHRLATIRNADHIYVLDDGRITEHGTHRQL
                     LDKQATYAALYAEADAPTLIGEKV"
     gene            5614..8367
                     /gene="brtM"
     CDS             5614..8367
                     /codon_start=1
                     /gene="brtM_rename1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     474.1; E-value: 1.5e-143)"
                     /gene_kind="biosynthetic"
                     /note="LanM"
                     /product="BrtM"
                     /protein_id="QKV49896.1"
                     /sec_met_domain="DUF4135 (E-value: 1.1e-73, bitscore:
                     237.6, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 2.5e-26, bitscore:
                     81.3, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINLEPSVSVPAFLPFYLDIAPKDDFAQSLVPVLDEVVSPGHRDA
                     VVHRLWNLLVCTVEGHSYRTLVGEFHAHREAMGLPADAGSRAAMESFTAALANPAQCRA
                     LLSRYPVLQQRLTTVTGNLRDACREMFEAYRDDIRVLSETFGIHPAGEAIIALDPSNSD
                     PHNGNRRVCFITTSGGTRLVYKPRSLAGDDCLRDLFRAAEDYLTHPLGTCVPESVTVDD
                     HGWQRFTEAEPMHDAGQASRYFYRFGAMTCLLSAVGASDLHDENVLAHGEYPCVIDTET
                     LLRGDSGVASDSLPHTLINHVKYSVTTTMLLPVEDPHSAIDVIMSGAGLVEEQQSKLRT
                     PVIAHQHSDAVQVEWKALTYRHTSNVPAVGDEKLSITDHFPHVMAGYRDALAFLRTGGL
                     EKTLADHPDISVRSVLRATEVYSRYLDASTHPKYLSSRAETRRLHGYLSNSPRDLPAQQ
                     IAYLQSHEEQALDAGDIPYFFTQASSTTLSTCAGSLPGFFKLSPLDNALRGVRAAAGRH
                     ERYHQFLVEECLGGVSSTPQGLSAHSVFAGDTLTKAAPGTWGFGITEVLHDLAITVEGS
                     RGTQAGWIGSIGPDRKAPTITPGNQIAFHDMGGIARLLRNAAKLNRRYVAWEQAADAGL
                     AELAITYRHVLDDMPESVFSGLASVLLTRPHVIDNGRMSELISRMEQRGHTREKDVANG
                     PAGVLMTVLSRIENGHEHEMRHVDALLRMSFGDARPALSGGDMELAHGPLGLYWAKART
                     GRILGDTALTDEAFDWLSARLDAHRPRAVGWCKGAAGLMVAAAEICQAAGRLEWLADGR
                     LTALVDTATALDDGPIELSVCHGTSGVIQALLSTAEFTDDPSLVDRARTYQNEVLKRAR
                     RSGFYTGVAGRTSLLGYMLGWSGIADTDLMLVSCAVNRRAPVALIG"
     gene            8379..9326
                     /gene="brtJ"
     CDS             8379..9326
                     /codon_start=1
                     /gene="brtJ"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083:oxidoreductase (Score: 123.3; E-value: 2.1e-37)"
                     /gene_kind="biosynthetic-additional"
                     /note="LanJc"
                     /product="BrtJ"
                     /protein_id="QKV49897.1"
                     /transl_table=11
                     /translation="MRYSLLLPIAVSRPEQAVPFANLVRWTRAQRFWQGQTYTIDNHHL
                     VSWLAGIGIRVPTGFGVNLTPLRSPYHAATEARSTALATGCSVLAAYGPGATSYQRALL
                     GRPYKSPLTATREYVTTVRALVRGESVKAEGAYCHTAARLVPAQSPPVKIGVGVLRPKM
                     AAVAGQIADAAVTWLCPPAYLRDVLIPHMHKSSAEAARDPVPVTAVVPCALAKDGRDMR
                     DMAFATVGQHLQAVHYQATLRHAGVEVTGRRTDADQLLGKDGFLYGTPSDIRDGLGRYA
                     DAGVDEVVLNVMGVAAIKGARAAVKELEEIFHVL"
     gene            9398..10315
                     /gene="abc"
     CDS             9398..10315
                     /codon_start=1
                     /gene="abc"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 157.2; E-value:
                     9.1e-48)"
                     /gene_kind="transport"
                     /note="ATP-binding cassette transporter"
                     /product="ABC transporter"
                     /protein_id="QKV49898.1"
                     /transl_table=11
                     /translation="MIQIEKLSKRYGDKVAVNDLTFTVAPGKVTGFLGPNGAGKSTTMR
                     MVLGLDRPSAGRALVDGRPYAELSQPLRTVGALLDAKGTHGARSAYQHLRALAASNGIP
                     DSRVGEVLGKVGLSSVSHQRARSFSLGMGQRLGIAAALLGNPKIVVLDEPVNGLDPEGV
                     VWVRELMKQLAADGCTVLVSSHLMNEMAVTADHLVIIAKGRLLVDSAMKDFIAQHSCHG
                     VLTRVANPQGLQDALAAEGIRNTRLPDGRIEAMEVSVEEVGRIAHSQQIAVYEMAPQTA
                     SLEEAFMKLTSQQTEYRAVLEAKQ"
     gene            10312..11040
                     /gene="abc"
     CDS             10312..11040
                     /codon_start=1
                     /gene="abc_rename1"
                     /note="ATP-binding cassette transporter"
                     /product="ABC transporter"
                     /protein_id="QKV49899.1"
                     /transl_table=11
                     /translation="MNNAMRSEWIKLISLRSYLWSLPSYFVVCLAVGSLMGYTSRRADN
                     LDPTALGFSGLQSGMLLMVVLGIMAVTNEYSSGTIQTSLLAVPRRITFYTAKLGTLMLV
                     TVVLSAVAAPGSFFLVQGILGDSGISVDATVLARMVGAVAYTTLLVGFSMGIAMVLRSS
                     TMTLSLLMPVFFMLSTILTNIPVTRKVAQFLPDMAGSLVLRQDLAPYSSLNAVTGLAVL
                     AAWAGVAATAGYISLARRDA"
ORIGIN
        1 atgtttttcg aaggtgacgt ttccctcatc gaggaaatcg ccgatcagga cttcgaaggt
       61 gtggcgtacg gcgcctgcac gacgaacacc ttctcgctca gcaacgcact gggaaacaat
      121 ggcggttggt gcacgctgac caaggaatgc cagcccaact gcaactgatg cagcggtgag
      181 caggttccca tccaccgaac cgaagggagg gtataggtat ggagcagcaa gacttcctgg
      241 gtgcctacga cgagcttgag ctgattgagc tcagcgagga ggaatggcat ggcggcaccg
      301 ggcccctgtg tgtagcggcg tcagtcgcgg cgtccgcggc tttctgtccg accaccaagt
      361 gcaccagcaa gtgctgacgc gctgagacgc cccacagaag gccatggggt ggggatcggt
      421 gcgtgatccc caccccatgt cgtccgggta ccctaccgca cctgtgtgca cggagtgaag
      481 tttccttgat ctcgccacag agcctttatc cagaattcga cagtgcagaa atacgggaaa
      541 cgataacacc gttgtcagca ttcgacggcg ctgtgctgga aatcctctcg aaacccgctc
      601 tcgtgaagga agcacagaaa gaggaactgc ccgaggttgc cgcccgatgg tcggccgaga
      661 atgaacagta ccccttccag cgtactgtcc gtgccctggc cgtccaggtg tgcaggaccg
      721 gtgtcgtgac cagacacgaa gccctcttca gtgactgcga cgagatcacc cgccagctgc
      781 tcagccgagt cgagagtcgc ctgaagaacg cctgcacgcg ctccctcgtc gctgcgatca
      841 acacggcttc ggcggacggg aagctgcgcg gcgagcacgg ggaagctcgc taccggcact
      901 ttctcgcgaa ctgcagcgct gagcaactgg gcgagtcctt cccccttctg ctctcggcag
      961 tcggacacct gctccgcaac acggctgccg ccttcgatga gctgtgcaca cggctgagcg
     1021 cagaccgtca gcgcatcgcc gagacgttcg gcatcgtcgg cagtgatgcc ctcacctcca
     1081 tcgggcaagc cgacggcgac gcccatgcgc acggacgcag cgtctccatc ctgacgttcg
     1141 cctcgggcgc ccggctggtg tacaagccgc gcgacatctc ctgcgaagcg gcctacgagg
     1201 ccatcgtcgc ccacgtcaac tcctgtgcgg gaaccagcct gttggctgcc agaaccctcg
     1261 accgtgcggg atacggctat gtggaataca tctccgccga ggacgtctcg ggcgtctccc
     1321 cgcagttcat gcgacgatgc ggtgagcttg ccgcggtgtt ccacctgctg gacgcccgtg
     1381 acatgcactt cgagaacatc gtcgccaccc gacgcgggcc ggtgcccgtc gacctcgaaa
     1441 cactcatgca tcccgcccgt gttcacgtcg gcccgcagcc ggaagcacct gacaatgcgt
     1501 acgacgtgat cgcccgttcc ctgtacggga tcggtatcct gccgctggtc atggccggaa
     1561 agggaaaaac ctcggggcac gtggacctgg gattcctggg aggagacaac cacggccctt
     1621 ctcctttcaa gggaatcgcc ttcgaaaatc ccttctcgga caagatcaga gttgtgctgc
     1681 gagccgagga gaccgggcag cgcagcaccg tggtgagcgc ggccgacgag gagaacatcc
     1741 atcaccttgc cgagcagatg gccgccggct tcaccaggac ggtgcgcgca ctgacacatc
     1801 agcgggaggc atggacccag ttgatgaccc ggctggcacg ccgcatgcgc gtcagatacg
     1861 tacataatcc gacggctctg tacatacaga acctgcgcct gatcgcttcc gccagtgcca
     1921 tggccgctcc cgccaccgct ctcggcctac tgaagcgaat tgcgatcgcg tcgaaaacct
     1981 cgcaccggag catcgtcgtc tccgagctca agcagatggc cgaacgcgat gtcccctact
     2041 ttaccgccaa ggcaacggac acggtgctgt atgacgccga cggcaatgac accggcgccc
     2101 ggttggcccg gacaccactg gcgcgggttc tggagaaggc agcttcgatc gacgaggccg
     2161 ccatcaccca gcaactctcc ttgctctact ctgccttctg ctcccggttc cccgacaacc
     2221 acctcactgg tgccgaagtc tggcccgccc gacccgcggt tgcccgctcg caggggctgc
     2281 tggatgtcgc acggagtctc gctgacgcgc tggtggccac cagcctgccg gacaagttcg
     2341 cgcacctgcc ccggacatgg atcggcccgc tggcctccgc ccaggcagaa cgtgcctggc
     2401 cgtgtggcgt tctcggctac gacctgtaca ccggtcgcac cggcccggcg ctggccctgg
     2461 ccgctgccgg gcgggtgctc ggcgacgacg catacaccga cgtagcccgg cagatcttca
     2521 ccactagtgc cgagatcctc gtcggtcggc gctacgagca gcgcagcgtc gaccagagcg
     2581 gtcccggcgc gtacaccgga ctggccggcc tgctgttcgc cttgcgcaca gcaggtgatc
     2641 tgttgcaaga accagactgg atccgggcgg cgcaggacgc cgttccgctg gtcgtcgagc
     2701 aggtcgacgg tgagagtgct gtggacgtca tcagcggtgt cgccggcatc gcggccgcga
     2761 tcgatgccat cggcggcacc catgccgaca acgtactacc cagcctgacc tcgatgctgt
     2821 gcacgatggc cgaacgcgcc cacggctcgt ggaccgagca atccggtttc gcccacggtg
     2881 tcgcgggtgt gctccacgcg ttgaacgtct tgcgtcctcg cgccggtgac cggagtctgc
     2941 gacagcgcgt cgacagggcc acgggcgtac tgctcgaccg gctggcggcg ttctacgacc
     3001 cgacggaagc caactggttc tccgacatca ctgcgcccgg ccggttctcc accggttggt
     3061 gtcacggagc cggcggtatc tccctggccc tgctggcgtg cgtgcagcat gacggggacg
     3121 agcgtgcccg ccggtggctc ggccaggcga ttaccaacac cgtcgagcac gggttcgggc
     3181 gcaacctgac gtggtgccat ggggacctcg gcaaccacgc cgttctgtcc gaagccgcca
     3241 cgaagctggg cgatgacgca ctggccgggc aggtgaggag tatcgaggac accggactac
     3301 gggccgaagt actccaggac aagctcactg accaccgcag ccggtacgcg cacaccaaca
     3361 gcgtgatggt cggttcctcg ggggttttgc tgcacctgct caacaggatc gaccccggcc
     3421 tgcggacctc tccgctgtca ctgtcttgtg gagcccgatg atggccaagc gcgtaccgac
     3481 agtcactcag gtgacacaga ccgagtgtgg tttgtgctgc tgtatctccg tcatgcgcta
     3541 ctggggccgc ggtgaggact tcttcaccgt ccgccaggac ttggaagccg gccgcgacgg
     3601 cctgggagcc aagcagctcg ccgacttcct gcgctcgcgc ggcatggaga ccaaggcgtt
     3661 ccgggccaag aacatcgatg cgctggcaca tttcaccgtt ccggtcatcc tgtactggga
     3721 ggagtaccac ttcctcgtcc tggagaagtt cgacggccgc accgcgacgg tcatggaccc
     3781 ggccgtaggg cgccggcgcc tttcacgcgc cgatctggaa gccgggttca gcgagatcgt
     3841 catcgtcccc gagcccggta ccgggttcac gtaccagagc ctgcccgcca ccaaggactg
     3901 gcgcaccgtc ccgctgttcg ccgaacgggc cgccgcgcgc atagctctgg tcgccatgct
     3961 gtccctcagc agctatctgg ccgccctggg cattcccatg ctgacgaagt gggcggtcga
     4021 ccggcaggcc cactggcggg acctggggga cctctcactg gtgatcgtgg tagttgtggc
     4081 agcggcggcc ggttatttgc tgctgtggct gctgcgggta acagctcttt ccgccctgat
     4141 ctcaatcttg gggcgtgcac tgatgactca cacgttccgc aagctgctct ccctgccgta
     4201 caagttcttc accacccgac aaccaggtga gctgctgttc cgcctcaaca ccgtcaacgt
     4261 ggtgcgcgac ctgctgtcgt cgcgcatcgc ccaaggcatc ctggacatcg gtacgctggt
     4321 gtgcatcagc gtctacctgt ttgtctccga gtggcgcatc ggtctggtcg ccgcgaccat
     4381 gttcgcactg aatgccgtgt atctatgggc cacccgcaac cgggtcaagg aggtcaccga
     4441 cgcggagatc agccaactgg gcagaagcca gtcggcgcag ctcgacgcca tcgtgtcgat
     4501 accgaccatc aagatgggcg gctacgcgca gcagttcgcc gacgagtggg ccaaggtcta
     4561 ccagtcatcg ctggatgcca tgaggcagcg catgagactc cagcaaggct ggatcgccgg
     4621 catcgccacc atgacgcaga tgttcggtcc agcggtgctg ctgctcacca gcttgtactt
     4681 cgtctcccaa ggagtggtct ccctgggcgc ggccatcgcg atccagtcgg tgtccatgac
     4741 ctacttctcc atgacgacgt cggtcttcca aatgttcacc gagttcaccg aggcctcccg
     4801 ctacatggcc cgactcagtg acatcaccgc atatacgagt gaaggagccg gtggtccgga
     4861 gcggcacctg gaggaggctt cggtcacgct gagggacgta cggttccagt acacacgcca
     4921 cgcggctcct gtggtcgacg gggtgtcgat gcacatcccg gccggatccc gggtggcgct
     4981 ggtgggggca tcgggatcag gcaagtccac acttgggcgg ctgatctgcg gcctgcacga
     5041 gccgaccggt ggagccatag agttcgcggg ccggccgatg gcggactacg acagggattt
     5101 tctccgacgc cagatcggct acatcccgca agaggtacac ctgcacaacc gcacgatttt
     5161 ggagaacctc acgctcggcc aggacatccc gcccgagaaa gtccgcgagt actgctctca
     5221 ggtcggcatt ctcgactttg tcgaggctct ccccatgggg ctgaagacac tggtgtcgga
     5281 gctcggggcc aatttctccg gcgggcagcg gcagcgcctc gcgatcgtca gaacactgct
     5341 gcagcagccg agaatcatcg tcatggacga ggccaccgcc tctctggaca ctctcaatga
     5401 acgccgcgtc accaagatca tcgaagatac gggcgccacc caagtgatca ttgcgcaccg
     5461 gctggccacc atacggaacg ccgaccacat ctacgtgctc gacgacggac gcatcaccga
     5521 acacggtacg caccggcaac tgctggacaa gcaggcgacc tacgcggctc tctacgcgga
     5581 agcggacgcc ccgacactca taggcgagaa agtatgatca acctcgaacc gtcggtttcc
     5641 gtacccgcct tcttgccgtt ctacctggac atcgccccca aggacgactt cgcccaatcc
     5701 cttgttccgg tgctggacga ggtggtgtct cccggccacc gggacgctgt cgtgcaccgg
     5761 ttgtggaacc tcctggtgtg cacggtcgag gggcacagct atcgcacact ggtcggcgag
     5821 ttccacgccc accgtgaagc aatggggcta ccggccgacg cgggcagcag ggctgccatg
     5881 gagtcgttca ccgctgcgct ggcgaacccg gcacaatgcc gagcgctgct gtctcgctat
     5941 ccagtgctcc agcagcggct gacgacggtc accggcaact tgcgcgacgc ctgccgggaa
     6001 atgttcgagg cgtaccggga cgacatccgg gtattgtccg agaccttcgg gattcacccg
     6061 gccggagagg ccatcatcgc gctcgacccg tccaactcgg acccgcacaa cggtaaccgg
     6121 cgcgtctgct tcatcaccac gagcggcggg acccgcttgg tgtacaagcc tcgctccctc
     6181 gccggggacg attgtctgcg agacctcttt cgcgcggccg aggattacct gacccatccg
     6241 ctcggcacct gcgtacccga gtcggtcacg gtcgacgatc acgggtggca gcgcttcacc
     6301 gaagccgaac cgatgcacga tgccgggcag gcatcacgct acttctaccg gttcggcgct
     6361 atgacctgct tgctgagcgc cgtcggggca tccgatttgc acgacgagaa cgtgctcgcg
     6421 cacggcgagt acccgtgtgt catcgacacc gaaaccttgc tcagagggga cagcggcgtg
     6481 gcgagcgact cgctgccgca caccctgatc aaccatgtga agtactccgt gaccaccacc
     6541 atgctgttgc cggtagagga cccacattcc gccatagacg tcatcatgtc gggcgcgggg
     6601 ctcgtcgagg agcagcagtc gaagctgcgc accccggtca tcgcccacca gcacagtgac
     6661 gccgtacagg tggagtggaa agcccttacc tatcgccaca ccagcaacgt cccggcagtc
     6721 ggcgatgaga agttgtcgat taccgatcat ttcccacacg tgatggccgg ttaccgcgat
     6781 gcccttgcgt tcttgcgcac gggcggactg gagaagacgc tggcagacca tcccgacatc
     6841 tccgttcggt ccgtcctgcg ggccacagag gtctacagtc gctacctgga tgcctccacg
     6901 caccccaagt acctctcttc ccgggccgag accaggcgcc tgcacggata tctgagcaac
     6961 agcccccgag acctccctgc ccagcagatc gcctacctgc agagccatga agaacaggca
     7021 ctcgacgcag gagacattcc ctacttcttc actcaggcgt cctcgacaac actctcgacg
     7081 tgcgccggca gcctgccagg tttcttcaag ctctcccccc tggacaacgc gctgcgcgga
     7141 gtgagagctg cggccggtcg gcatgagcgg taccaccagt ttctcgttga ggagtgcctc
     7201 ggtggtgtca gcagtacccc gcagggtctg tcggcgcaca gtgtgttcgc cggtgacacg
     7261 ctgaccaaag cggcaccggg cacctggggc ttcggcatca ctgaggtgct ccacgacctg
     7321 gccatcaccg tcgagggctc ccggggaaca caggccggct ggatcggcag catcggcccg
     7381 gaccgaaaag cccccacgat caccccaggt aaccagatcg cctttcatga catgggcggc
     7441 atcgctcgac tgttgcgaaa cgcagccaag ctgaaccggc gctacgtggc ttgggagcag
     7501 gctgcggacg caggcctcgc cgaactggcg atcacgtatc ggcacgtgtt ggacgacatg
     7561 cccgaatcgg tgttctccgg gctcgcctcg gtactgctca cccggccgca tgtcatcgac
     7621 aacggccgga tgagcgaatt gatctcccgc atggaacagc ggggccacac cagggagaaa
     7681 gacgtcgcca acggcccagc aggcgtgctc atgaccgtgc tctcccgcat cgaaaacggc
     7741 cacgagcacg agatgcggca cgttgacgct ctactccgaa tgtccttcgg tgacgcccgc
     7801 cccgctctgt cgggcggcga tatggaactg gcccacgggc ccctggggct gtactgggcc
     7861 aaggcccgta ccggccgcat cctcggcgac acagcgctga ccgacgaggc gttcgactgg
     7921 ttgtctgccc gtctggacgc acaccggccc cgagccgtcg gttggtgcaa gggagcagcc
     7981 ggcctcatgg tggctgccgc cgagatctgc caggccgccg gtcgccttga gtggctcgcc
     8041 gacgggcggc tgaccgcgct ggtcgacaca gcgaccgcgc tggacgacgg accgatagaa
     8101 ctctcggtgt gccatggcac cagcggcgtg atccaggcac tcctttccac agcggaattc
     8161 accgatgacc cctctctcgt cgaccgggcc cgtacctacc agaacgaggt gctcaagcgt
     8221 gcgcgccgaa gcggcttcta caccggtgtg gcgggccgca cctcgttgct cggctacatg
     8281 ctcgggtggt ccggcatcgc cgacaccgat ctgatgctcg tctcctgcgc agtgaaccgc
     8341 cgggcgcctg tcgcgctgat cggttaggag gaacaaccat gcgctactcg cttctcctgc
     8401 ccatcgccgt atcccggccg gagcaagccg tccccttcgc gaacctggtg cggtggaccc
     8461 gtgcccagcg cttctggcag ggccagacct acaccatcga caaccatcac ctggtcagct
     8521 ggctggccgg aatcggcata cgcgtcccca ctggattcgg tgtgaacctg actcctctac
     8581 ggtccccgta tcacgcggcg accgaggccc gctcgaccgc attggccacc ggctgctcgg
     8641 tcctggctgc gtacgggccg ggcgccacga gctaccagcg cgccctcctg gggcgccctt
     8701 acaaaagtcc tctgaccgcc acacgggaat atgtcacgac ggtgcgtgca ctggtgagag
     8761 gcgagtcggt aaaagcggaa ggtgcctact gccatacggc tgctcggctc gttcccgctc
     8821 agtctccacc ggtgaagatc ggcgtcggcg tactgcgtcc caaaatggcc gcggtagccg
     8881 gacagatagc agacgccgca gtcacctggc tgtgtccgcc cgcctacctg cgcgacgttc
     8941 tcattccgca tatgcacaag agcagtgccg aggcggcacg tgacccggta ccggtcacgg
     9001 ccgtggtccc gtgcgcactc gccaaagatg gccgtgacat gcgggacatg gcattcgcga
     9061 cggtcggtca gcaccttcaa gccgtccact accaggccac cctcagacac gcgggagtcg
     9121 aagtgaccgg ccgacgcaca gacgcagacc aactgctcgg caaggatgga ttcctgtacg
     9181 gcacaccctc ggacatacgt gacgggctcg gtaggtatgc cgacgctgga gtcgatgaag
     9241 tagtcctcaa cgttatgggt gttgctgcca tcaaaggtgc gcgtgcggcg gtcaaggaac
     9301 tcgaagaaat tttccacgtg ctgtgatctt ttcgcccgtc agcaggggcg gactccgaag
     9361 caagttatcc accgaccaac cactgcaatg gatactgatg attcaaatcg agaagctgag
     9421 caagcgctac ggggacaagg tcgccgtgaa tgacctgacc ttcaccgtcg cgcccggcaa
     9481 agtaaccggc ttcctcggcc ccaacggggc gggcaagtcc accaccatgc gcatggtctt
     9541 ggggctcgac cgcccgagcg ctggacgcgc cctcgtggac ggtaggccgt acgcggagct
     9601 ctcccagccg ctgaggacgg tcggagcgct gctcgacgcc aagggcaccc acggtgcccg
     9661 tagcgcctac cagcacctac gcgcgctcgc cgcgagcaac ggcatccccg actctcgcgt
     9721 aggcgaggtt ctgggaaaag taggcctgtc gagcgtgtcc caccagcggg ccagaagctt
     9781 ttccctcggc atgggccagc ggctgggcat cgcagccgcc ctgctcggca acccgaagat
     9841 cgtcgtactc gacgagccgg tcaacggcct cgaccccgaa ggcgtcgtat gggtgcgtga
     9901 gctgatgaag caactcgcag ccgacggatg caccgtgctg gtgtcatccc acctgatgaa
     9961 cgagatggcc gtcaccgctg accatctggt gatcatcgcc aaggggcgac tgctggtcga
    10021 ttccgccatg aaggatttca tcgcccagca ttcctgccac ggcgtgctga cccgtgtcgc
    10081 gaacccccag ggcctgcaag atgccctggc cgccgaaggc atcaggaata cgcgcctgcc
    10141 cgacggccgg atcgaagcca tggaagtaag tgtggaagag gtcggccgga ttgcccactc
    10201 ccagcagatc gccgtctatg agatggcacc gcaaacggcg tccttggaag aggcattcat
    10261 gaagctgacc tcacagcaga ccgaataccg ggccgtgctg gaggcgaagc aatgaacaac
    10321 gccatgcggt cggaatggat caagctgatc agcctgaggt cttacctctg gtcactgccg
    10381 tcctacttcg tcgtctgcct cgcagtcggc agcctgatgg gatataccag ccgtagagcc
    10441 gacaacctcg atccgaccgc gctgggcttc tccggcctgc aaagcggaat gctgctgatg
    10501 gtggttctcg gcatcatggc ggtcaccaac gagtactctt ccggcactat tcagacctcg
    10561 ctgctcgcgg taccacgacg catcacgttt tacacggcga aactggggac cctcatgctt
    10621 gtgacggtcg tcctgtcagc cgtcgccgca ccggggagtt tcttcctcgt gcagggcatt
    10681 ctcggtgatt ccggcatcag cgtcgacgcc acggtacttg ctcgtatggt cggcgcggtc
    10741 gcgtacacca cgctcctggt gggtttctcg atgggcattg ccatggtgct gcgatccagc
    10801 accatgacgc tgagcctgct catgccggtg ttcttcatgc tgtcgacgat cctgaccaac
    10861 attccggtga ctcgaaaggt cgcacagttc ttgccggaca tggctggatc cctggtcctg
    10921 cggcaggacc tggctcccta cagcagcctc aacgccgtga ccgggctggc agtactagcc
    10981 gcatgggcgg gggttgcagc gacagccggt tacatatcgc ttgcccgccg cgacgcttga
//
