BGC0002124 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002124 (Actinomadura verrucosospora)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	143,469	High	SF2487	PKS:Type I

BGC0002124 - Region 1 - T1PKS

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Location: 1 - 143,469 nt. (total: 143,469 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 143,470
Label		BGC0002124

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

ACTIVE_1774: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

ACTIVE_1775: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

ACTIVE_1786: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

ACTIVE_1792: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

ACTIVE_1794: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

ACTIVE_1795: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Transferases: PPTase acylase

ACTIVE_1781: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

ACTIVE_1802: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1060: 4'-phosphopantetheinyl transferase

Hydrolases: esterase hydrolase phosphatase

ACTIVE_1791: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

ACTIVE_1798: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

ACTIVE_1799: thioesterase

smcogs: SMCOG1004: thioesterase

ACTIVE_1805: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Lyases: cyclase

ACTIVE_1773: Diels-Alderase cyclase

extras: DA_cyclase: Diels-Alderase cyclase

Ligases: synthase

ACTIVE_1772: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

ACTIVE_1780: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ACTIVE_1783: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ACTIVE_1784: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

ACTIVE_1789: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

ACTIVE_1796: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ACTIVE_1800: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002124.3	1.00	PKS	SF2487	Actinomadura verrucosospora NRRL:B18236
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002175.3	0.66	PKS	YWA1	Aspergillus oryzae RIB40
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001160.5	0.66	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002124.3	1.00	PKS	SF2487	Actinomadura verrucosospora NRRL:B18236
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002175.3	0.66	PKS	YWA1	Aspergillus oryzae RIB40
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001160.5	0.66	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 143469:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - ccmal - redmal) + (ccemal - Me-redmal) + (ccmal) + (Me-ccmal - Me-ohmal) + (redmal - Me-ccmal - ccmal) + (Me-redmal - Me-ccmal - Me-ccmal) + (ohmal)
Non-colinear ordering:: ACTIVE_1800 + ACTIVE_1772 + ACTIVE_1780 + ACTIVE_1781 + ACTIVE_1783 + ACTIVE_1784 + ACTIVE_1796

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ACTIVE_1772: emal - mmal

PKS_AT (577..874): emal

ATSignature: Ethylmalonyl-CoA, Methoxymalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%; Methylmalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 212.9
Methylmalonyl-CoA: 205.5
Methoxymalonyl-CoA: 177.9
2-Methylbutyryl-CoA: 145.6
trans-1,2-CPDA: 127.7
Propionyl-CoA: 117.3
Malonyl-CoA: 105.1
Isobutyryl-CoA: 105.1
CHC-CoA: 104.3
Benzoyl-CoA: 68.5
fatty_acid: 56.0
Acetyl-CoA: 54.9
3-Methylbutyryl-CoA: 46.1
inactive: 32.6

PKS_AT (2362..2657): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 187.4
Ethylmalonyl-CoA: 181.3
Methoxymalonyl-CoA: 161.9
2-Methylbutyryl-CoA: 127.1
trans-1,2-CPDA: 120.1
Malonyl-CoA: 109.3
Propionyl-CoA: 107.0
CHC-CoA: 101.2
Isobutyryl-CoA: 59.2
Benzoyl-CoA: 49.1
Acetyl-CoA: 37.1
inactive: 33.3
fatty_acid: 24.0
3-Methylbutyryl-CoA: 10.1

ACTIVE_1780: mal

PKS_AT (558..845): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 215.2
inactive: 111.4
Methoxymalonyl-CoA: 54.0
Methylmalonyl-CoA: 44.2
Propionyl-CoA: 43.0
2-Methylbutyryl-CoA: 26.5
Ethylmalonyl-CoA: 22.2
Acetyl-CoA: 19.6
Isobutyryl-CoA: 17.4
fatty_acid: 12.5
Benzoyl-CoA: 9.7
trans-1,2-CPDA: 8.6
CHC-CoA: 7.4
3-Methylbutyryl-CoA: 0.0

ACTIVE_1781: mmal - mmal

PKS_AT (565..837): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 195.2
Ethylmalonyl-CoA: 185.4
Methoxymalonyl-CoA: 162.5
2-Methylbutyryl-CoA: 139.2
Propionyl-CoA: 126.9
trans-1,2-CPDA: 124.1
Malonyl-CoA: 116.0
CHC-CoA: 107.6
Isobutyryl-CoA: 65.2
Benzoyl-CoA: 58.0
Acetyl-CoA: 47.5
inactive: 31.2
fatty_acid: 30.5
3-Methylbutyryl-CoA: 26.9

PKS_AT (2290..2584): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 209.4
Ethylmalonyl-CoA: 194.4
Methoxymalonyl-CoA: 169.3
2-Methylbutyryl-CoA: 156.0
trans-1,2-CPDA: 133.4
Propionyl-CoA: 114.6
Malonyl-CoA: 102.5
CHC-CoA: 94.4
Isobutyryl-CoA: 66.3
Benzoyl-CoA: 58.0
Acetyl-CoA: 46.8
3-Methylbutyryl-CoA: 32.3
inactive: 30.6
fatty_acid: 27.3

ACTIVE_1783: mal - mmal - mal

PKS_AT (571..865): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 160.9
inactive: 74.8
Methoxymalonyl-CoA: 55.2
Methylmalonyl-CoA: 50.7
Propionyl-CoA: 29.0
Isobutyryl-CoA: 28.3
Ethylmalonyl-CoA: 23.1
2-Methylbutyryl-CoA: 16.7
Acetyl-CoA: 13.0
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2641..2934): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 186.8
Ethylmalonyl-CoA: 178.2
Methoxymalonyl-CoA: 158.0
2-Methylbutyryl-CoA: 132.7
trans-1,2-CPDA: 114.8
Malonyl-CoA: 106.3
Propionyl-CoA: 103.1
CHC-CoA: 97.7
Isobutyryl-CoA: 67.9
Benzoyl-CoA: 58.2
Acetyl-CoA: 38.7
inactive: 30.9
fatty_acid: 26.8
3-Methylbutyryl-CoA: 18.7

PKS_AT (4382..4664): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 160.9
inactive: 74.8
Methoxymalonyl-CoA: 55.2
Methylmalonyl-CoA: 50.7
Propionyl-CoA: 29.0
Isobutyryl-CoA: 28.3
Ethylmalonyl-CoA: 23.1
2-Methylbutyryl-CoA: 16.7
Acetyl-CoA: 13.0
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

ACTIVE_1784: mmal - mmal - mmal

PKS_AT (534..827): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 185.4
Ethylmalonyl-CoA: 167.1
Methoxymalonyl-CoA: 150.8
2-Methylbutyryl-CoA: 126.8
Malonyl-CoA: 119.9
trans-1,2-CPDA: 108.7
Propionyl-CoA: 99.3
CHC-CoA: 92.1
Benzoyl-CoA: 64.8
Isobutyryl-CoA: 64.6
Acetyl-CoA: 48.9
inactive: 39.4
fatty_acid: 23.6
3-Methylbutyryl-CoA: 10.7

PKS_AT (2598..2891): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 177.6
Ethylmalonyl-CoA: 166.0
Methoxymalonyl-CoA: 151.1
2-Methylbutyryl-CoA: 126.9
trans-1,2-CPDA: 105.9
Propionyl-CoA: 100.2
Malonyl-CoA: 92.6
CHC-CoA: 91.7
Isobutyryl-CoA: 65.3
Benzoyl-CoA: 53.6
inactive: 27.7
Acetyl-CoA: 27.2
fatty_acid: 20.3
3-Methylbutyryl-CoA: 9.2

PKS_AT (4306..4607): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Malonyl-CoA: 83.3%; Methoxymalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 198.0
Ethylmalonyl-CoA: 196.1
Methoxymalonyl-CoA: 163.5
2-Methylbutyryl-CoA: 138.2
trans-1,2-CPDA: 109.7
Propionyl-CoA: 106.7
CHC-CoA: 100.7
Malonyl-CoA: 97.5
Isobutyryl-CoA: 77.5
Acetyl-CoA: 66.2
Benzoyl-CoA: 50.4
fatty_acid: 27.7
3-Methylbutyryl-CoA: 27.5
inactive: 20.4

ACTIVE_1796: mal

PKS_AT (581..868): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 146.5
inactive: 88.0
Methoxymalonyl-CoA: 48.2
Methylmalonyl-CoA: 30.5
Isobutyryl-CoA: 28.0
Propionyl-CoA: 25.1
Acetyl-CoA: 10.8
2-Methylbutyryl-CoA: 9.4
CHC-CoA: 7.9
Ethylmalonyl-CoA: 1.1
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

ACTIVE_1800: mal - mal - mal

PKS_AT (521..819): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 70.8%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 194.6
inactive: 96.4
Methoxymalonyl-CoA: 73.8
Methylmalonyl-CoA: 53.4
Isobutyryl-CoA: 31.7
Propionyl-CoA: 21.1
Benzoyl-CoA: 20.9
Ethylmalonyl-CoA: 18.4
trans-1,2-CPDA: 14.7
Acetyl-CoA: 13.9
fatty_acid: 9.1
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (1532..1834): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 200.4
inactive: 93.1
Methoxymalonyl-CoA: 47.6
Methylmalonyl-CoA: 28.9
Isobutyryl-CoA: 19.9
Propionyl-CoA: 16.6
Benzoyl-CoA: 11.9
Acetyl-CoA: 11.9
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (3252..3546): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 75.0%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 215.6
inactive: 101.0
Methoxymalonyl-CoA: 55.2
Methylmalonyl-CoA: 44.6
Propionyl-CoA: 26.2
Isobutyryl-CoA: 23.7
Benzoyl-CoA: 21.9
Acetyl-CoA: 15.5
fatty_acid: 11.7
Ethylmalonyl-CoA: 10.0
CHC-CoA: 8.5
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor