LOCUS       BGC0002132              8455 bp    DNA     linear   CON 02-AUG-2022
DEFINITION  Methylosinus sp. LW4 MetLW4DRAFT_scaffold2.2, whole genome shotgun
            sequence.
ACCESSION   BGC0002132
VERSION     BGC0002132
KEYWORDS    .
SOURCE      Methylosinus sp. LW4
  ORGANISM  Methylosinus sp. LW4
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Methylocystaceae; Methylosinus.
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            KB900627.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 404043
            Source DNA and Organism available from Ludmila  Chistoserdova
            (milachis@u.washington.edu)
            Source DNA available from Ludmila  Chistoserdova
            (milachis@u.washington.edu)
            Organism available from Ludmila  Chistoserdova
            (milachis@u.washington.edu)
            Contacts: Ludmila  Chistoserdova (milachis@u.washington.edu)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 17:00:08
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 8455
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..2250)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614772.1"
                     /locus_tag="METLW4_RS0121355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_018268272.1"
                     /transl_table=11
                     /translation="MADVNSIRPDAVVEAAPEPDETALATEERQKPLLRAFARIRPLFA
                     APPSIDIGRVAAIVVVPVFCWVFFTTSSGMIDIMRRESSDLVGVVGAIIGTSAILVMLA
                     ATSWSLGADLGALIARRQIFGERMLVKTSVTAIVYLFVFSISAFFSFTYYYTNIFRLSS
                     KKMVAELQPMALAADVLLPAAEAAAADYDAETARLNATAGMRAYQDSLEAALQAANDAA
                     ARLRDGLEKGREERRRAASELARKRASEIEEAQAAARQLAETRERLAALEHAAADLEPI
                     IRAKQDEIVALTSTARQEDQLAVDASKGLDGLGAACGANCESHRGKANAARKRIQTIQD
                     TLAAPTGRRDEAIRQRDMLAAQIVTLRQKAESAPSAHAAAAVAGEEPADIASAARRLAL
                     ARDLIRADPSWARIREAKPACEAIVEAMRAAGVKHGGVAAEFDCEPASVETRDRLTTRD
                     ETIAGRATFERKCSLDGEIRGEMSAIATRIRNAADADTASAAGGFEDAKKIVDDCIVMA
                     KAAGLTEAEVRSYLERSDIFLRTHSMQRNRFELAREAFVGLTPDATMAIGVAVAQDAFM
                     FVMKLLSEIFKARDSQSRAPAPALPALDVADSDGEAADLRLMKALLRLSRPRPGGRSAF
                     DAAEIADLPIDVRSNIVGLLNRLVREGVASLDRKGVYLLDNRTLAEVEAKLSLALKRAS
                     RGSVAAEVAALTAELGAGDGSARGYWRRRRSALDRYLTLRAASSVDAESEEAAAE"
     protocluster    1..8455
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[4034:5427]"
                     /cutoff="5000"
                     /detection_rule="(DUF692 and MbnC)"
                     /neighbourhood="10000"
                     /product="methanobactin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      4035..5427
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(DUF692 and MbnC)"
                     /neighbourhood="10000"
                     /product="methanobactin"
                     /protocluster_number="1"
     cand_cluster    1..8455
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(DUF692 and MbnC)"
                     /kind="single"
                     /product="methanobactin"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..8455
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002132"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..8455
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="methanobactin"
                     /region_number="1"
                     /rules="(DUF692 and MbnC)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1..2250)
                     /locus_tag="METLW4_RS0121355"
     gene            complement(2734..4038)
                     /locus_tag="METLW4_RS0121360"
     CDS             complement(2734..4038)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="METLW4_RS0121360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_018268273.1"
                     /transl_table=11
                     /translation="MIESDKGFWRGYWPLLLVIVAGQLAQQADVIMVGHLGGGAPGAYA
                     MTTRLVVVDVILLSALGAVASTAIGEARRSGASPRVIGAILWLSLFAGLCCAAFGSASY
                     PRFARLLVDDDAIAGLICDGVLWYSFAAPFRFVSSTSAFMLHALDQGSGVVKWKLWEFA
                     AKAIGNFLAMEIFGLGFSSCFQIGAVICVGSSIWGVWALSRHGVDWRTLPGASWARRFG
                     KAALWESERGVAMHLAMLACLAMFAAPGLGNYHVGRLNCFAAGQTLMLIVFTPLVTLMR
                     FLAFRLAGLTDERLIDSIRTISLKGAPFAVGVAILLMAGADALGRLYGQHGPWWTTLVE
                     ILAASIPLRYMTSILRAVLQARGSFGAVAKTDSSVFWFVALPLVALGLYIDSPFVAYLS
                     LIVPEGLSALRFWKQAPLVSRAARPRSSAETSTAI"
     gene            complement(4035..4625)
                     /gene="mbnC"
                     /locus_tag="METLW4_RS0121365"
     CDS             complement(4035..4625)
                     /codon_start=1
                     /gene="mbnC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     methanobactin: MbnC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921439.1"
                     /locus_tag="METLW4_RS0121365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="methanobactin biosynthesis cassette protein MbnC"
                     /protein_id="WP_018268274.1"
                     /sec_met_domain="MbnC (E-value: 1.5e-67, bitscore: 216.0,
                     seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLLSPAPARIDADLYQDMMNPSRLDAFARESRSHVRLDISYRAY
                     WHVLFDAVPQLLELSGPDGRAIFLPFMQWAQEKNLTFDWAYHIWAAEWLLQSEFRERVG
                     ADLALKLMAAAASRWSGRDRDLDYCGIVIASRLIGPTKAVAGWKLSSIETRFIEVEQVE
                     FECELPAPEDNFGCFRTPGYELDHFPGWRPIPL"
     gene            complement(4597..5427)
                     /gene="mbnB"
                     /locus_tag="METLW4_RS0121370"
     CDS             complement(4597..5427)
                     /codon_start=1
                     /gene="mbnB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     methanobactin: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921438.1"
                     /locus_tag="METLW4_RS0121370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="methanobactin biosynthesis cassette protein MbnB"
                     /protein_id="WP_018268275.1"
                     /sec_met_domain="DUF692 (E-value: 6.2e-21, bitscore: 63.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQIGFNFTLNGTLDMVRRMLRERQIDYCELLIDNFLNVPVKELAD
                     AFECPVGFHIMLSKFLENDRETLEKLAKQIRILIDALQPAYVSDHLLRFAPNGRNLFYL
                     GEIDYAQYAPVRNRVEQWQDMLGVQLHLENYPSIMDGGWEQPEFFRRLCRETGAGILFD
                     ASNAVVARHNCGAPLDAWNDVISSTRHFHVAGYGPSFLDPRVIVDSHDREMAPDTLEFL
                     RSMRAAFDKPGSTITYERDFEIEYDSIAIDLERLREIFPHAEDTTNESLVACAG"
     gene            complement(5512..5607)
                     /gene="mbnA"
                     /locus_tag="METLW4_RS28040"
     CDS             complement(5512..5607)
                     /codon_start=1
                     /gene="mbnA"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="METLW4_RS28040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="methanobactin"
                     /protein_id="WP_157235562.1"
                     /transl_table=11
                     /translation="MTIKVVKKEILPVIGRVQAMCACNPPWCGTC"
     gene            complement(5721..6770)
                     /locus_tag="METLW4_RS0121375"
     CDS             complement(5721..6770)
                     /EC_number="2.6.1.9"
                     /NRPS_PKS="Domain: Aminotran_1_2 (12-335). E-value:
                     1.4e-38. Score: 124.8. Matches aSDomain:
                     nrpspksdomains_METLW4_RS0121375_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019:aminotransferase (Score: 130.5; E-value:
                     1.3e-39)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614732.1"
                     /locus_tag="METLW4_RS0121375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="histidinol-phosphate transaminase"
                     /protein_id="WP_018268276.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 2e-39, bitscore:
                     124.8, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYGAIPRGDGLCLDANEMRFPLPDEFLARVAREISTIDFRRYPDA
                     MEIDALEAAIAANIGVRAKNILLGVGSTEMLDLVCRAFGGPFEAVVVPTPTFPLYGHFA
                     ALNDKGFVTVPLDDAFALSTEVVDKMTAVDGAKIFYVGNPNNPTGRALDRAIFERLAVS
                     TSDLLCLDEAYSAYADVSYATDAIDRHNVIVAQSFSKIGLAGCRLGYLVASEPLIDELE
                     RASLPYRVNAMTACVARIFLEETALFQPLIDQVKRERERLREELARMPGFSPFRSEGNF
                     LFCATPRTGASVAEELRERFRIFVKAFPPTSRIGKHLRITVGTPSENDALLEALASLSP
                     VESRESLST"
     aSDomain        complement(5766..6734)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_METLW4_RS0121375_Aminotran_1_2.1
                     "
                     /evalue="1.40E-38"
                     /label="METLW4_RS0121375_Aminotran_1_2.1"
                     /locus_tag="METLW4_RS0121375"
                     /protein_end="335"
                     /protein_start="12"
                     /score="124.8"
                     /tool="antismash"
                     /translation="LDANEMRFPLPDEFLARVAREISTIDFRRYPDAMEIDALEAAIAA
                     NIGVRAKNILLGVGSTEMLDLVCRAFGGPFEAVVVPTPTFPLYGHFAALNDKGFVTVPL
                     DDAFALSTEVVDKMTAVDGAKIFYVGNPNNPTGRALDRAIFERLAVSTSDLLCLDEAYS
                     AYADVSYATDAIDRHNVIVAQSFSKIGLAGCRLGYLVASEPLIDELERASLPYRVNAMT
                     ACVARIFLEETALFQPLIDQVKRERERLREELARMPGFSPFRSEGNFLFCATPRTGASV
                     AEELRERFRIFVKAFPPTSRIGKHLRITVGTPSENDALLEAL"
     gene            7451..7708
                     /locus_tag="METLW4_RS28620"
     CDS             7451..7708
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="METLW4_RS28620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_245258515.1"
                     /transl_table=11
                     /translation="MRIRVGALVIFADCFDDLAGTFRTAFGVGFVSCMSEAVAERRYVA
                     RSVETRRDGGAAVVKRLRDRAGASLAAADINGESRGPVRV"
     gene            complement(7958..8455)
                     /locus_tag="METLW4_RS26715"
     CDS             complement(7958..8455)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032:RNA
                     polymerase, sigma-24 subunit, ECF subfamily (Score: 95.1;
                     E-value: 6.2e-29)"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /locus_tag="METLW4_RS26715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="RNA polymerase sigma factor"
                     /protein_id="WP_157235566.1"
                     /transl_table=11
                     /translation="MTAKEKSVQQLYRDYAPRLRQFASRRLGWEESEDIVQEAYLRLVK
                     EGAEKLTCPRAYLYRITANLVTDMHRRRKLRSAFTDAATPFDESVHSDTPNVAEPHDYS
                     STFEQLSPLRKQIILLKVVDGATNIEIADRCGVSTRTVERHLTKARGDLRDSMAFQSSN
                     PA"
ORIGIN
        1 ctattcggcg gccgcttcct cgctctcggc atcgacggac gaggcggcgc gcagcgtcag
       61 atagcggtcg agcgcgctgc gccggcggcg ccagtagcca cgcgccgatc cgtccccggc
      121 gccgagctcg gcggtgagcg cggcgacctc cgccgcgacg gagccgcgcg acgcgcgctt
      181 cagcgcgagc gagagcttcg cctccacctc ggccagcgtg cgattatcga gcaggtagac
      241 gcccttgcgg tcgaggctcg ccactccctc ccgcaccagc cggttcagca ggccgacgat
      301 attgctgcgc acgtcgatcg gcagatcggc gatctcggcc gcgtcgaagg cgctgcggcc
      361 gcccgggcgc ggccgcgaga gccgcagcag cgccttcatc aggcgtagat ccgcggcctc
      421 gccgtcgctg tccgcgacat cgagcgcggg cagagccggc gccggcgcgc gcgactgcga
      481 atcgcgcgcc ttgaagattt ccgacagcag cttcatcacg aacatgaagg cgtcctgcgc
      541 cacggccacg ccgatcgcca tggtcgcgtc cggcgtgagg ccgacgaagg cctcgcgcgc
      601 cagctcgaag cgattgcgct gcatgctgtg cgtgcgcagg aagatgtcgc tgcgctcgag
      661 atagcttcgt acctccgctt ccgtgagacc ggccgccttg gccatcacga tgcaatcgtc
      721 gacgatcttc ttggcgtcct cgaagccgcc ggcggcggag gccgtgtcgg cgtcggccgc
      781 gttgcggatg cgcgtggcga tggcgctcat ctcgccgcga atctcgccgt cgagcgagca
      841 tttgcgctcg aatgtggcgc ggccggcgat cgtctcgtcc cgcgtcgtga gccggtcgcg
      901 cgtctcgacg ctcgcgggct cgcaatcgaa ttcggcggcg acgccgccgt gcttgacgcc
      961 cgcggcccgc atggcctcga cgatcgcctc gcaggccggc ttggcctcgc gaatccgcgc
     1021 ccagctcgga tcggcgcgaa tgagatcgcg cgcgagggcg aggcggcgcg cggcgctcgc
     1081 gatatcggcg ggctcctccc ccgccacagc ggcggcggcg tgcgcggagg gcgcgctctc
     1141 cgccttctgc cgcagcgtga cgatctgcgc cgcgagcatg tcgcgctgcc ggatcgcctc
     1201 gtcgcgacga ccggtcggcg ccgcgagcgt gtcctgtatc gtctgaatgc gcttgcgcgc
     1261 cgcattggcc ttgccgcgat gcgactcgca atttgcgccg caggcggcgc cgagcccgtc
     1321 gaggcccttc gacgcgtcga cggcgagctg gtcctcctgg cgcgccgtcg aggtgagcgc
     1381 gacgatctcg tcctgcttgg cgcggatgat cggctcgagg tcggcggcgg catgctcgag
     1441 cgcagcgagc ctttcgcgcg tctccgccaa ttgccgcgcc gccgcctgcg cctcctcgat
     1501 ctcggaggcg cgcttgcggg cgagctcgga ggccgcgcgc cggcgctcct cgcgaccttt
     1561 ctccagcccg tcgcgcaggc gcgccgcagc gtcattggcc gcctgcagcg cagcctcgag
     1621 cgaatcctga taggcgcgca tgcccgccgt cgcattgagg cgcgccgtct cagcgtcgta
     1681 atcggccgcc gcggcctcgg cggccggcag cagcacgtcg gcggcgagcg ccatgggctg
     1741 cagctcggcc accatcttct tcgacgagag ccggaatatg ttcgtgtagt agtaggtgaa
     1801 ggagaagaag gccgagatgg agaagacgaa gagatagacg atcgccgtga cgctcgtctt
     1861 gacgagcatt cgctcgccga agatctgccg ccgcgcgatc agcgcgccga gatcggcgcc
     1921 gagcgaccag ctcgtcgcgg ccagcatgac gagtatggcc gatgtgccga tgatcgcgcc
     1981 gacgacgccg acgaggtcgc tcgactcgcg gcgcatgatg tcgatcatgc cgctcgatgt
     2041 ggtgaagaac acccagcaga atactggaac gacgacgatg gcggcgacgc ggcctatgtc
     2101 gatcgacggc ggagcggcga aaagcgggcg aatgcgcgcg aacgcacgga gcagcggctt
     2161 ctgccgctct tccgtcgcga gcgcggtttc atctggctca ggcgccgcct cgacgacggc
     2221 gtccggtcga atcgaattga cgtcggccat gcccggctcc ctcgcgcgct ccgcatctgc
     2281 atcgaagcca aacttagcat agagccggga atgcttgaac gcgccggcgc gccctcgtcg
     2341 ttcgacgctg tttccgcgcc catttcgggc gcgcgggatg gtcctgggcg cggcgtcgcc
     2401 gctcgtcgat gtccgagcag catggccgac agcggatcgc ccatgagcag ccggagcgca
     2461 agaccgccga tcacatactc ggatagcgat gcgcgggggc gacgcgcgac aactccaggg
     2521 acgcgaggag cggatgaaac cagccgctgc cgagcgagcg atggcgaggc gagccggcag
     2581 ccgagcgtgg gccgcgccga gcgcatcgtg agatgcgcgc cggccagatc gacggcgggt
     2641 agaggtgtgt gaatttcgcg gaacgcgcct ctggaaggac aagcgtgcgg ccatttcgcc
     2701 gtattgcgag cccatgcgac ccgatacgcg gcgtcagatc gccgtcgatg tttcggcgct
     2761 ggaccgcggc cgcgcggccc gcgagacgag aggcgcctgc ttccaaaagc gcaatgcgct
     2821 gagaccttcc gggacgatca gcgacaaata ggcgacgaaa ggcgaatcga tataaagtcc
     2881 gagcgcgacg agcggcaagg cgacgaacca aaacactgag ctatccgttt tcgccaccgc
     2941 cccgaacgac cctcgagcct gcaagacggc tctgaggatc gacgtcatgt agcgcagcgg
     3001 tatcgatgca gccagaattt cgaccaaggt cgtccaccaa ggaccatgct gaccatataa
     3061 tcgcccaagc gcgtcggctc cggccatgag caagatcgca acgcccacgg cgaagggagc
     3121 gcccttcagc gagatcgttc tgatcgaatc tatgaggcgt tcgtcagtga gccctgcaag
     3181 gcgaaatgcg agaaagcgca tcaaagtgac gagcggcgtg aaaacgatca acatcaatgt
     3241 ctgacccgcg gcgaagcaat tcagcctccc gacgtgataa ttgccgagtc cgggcgccgc
     3301 gaacatggcg aggcatgcga gcatagcgag atgcatggcg acgccccgct cggactccca
     3361 gagcgccgct ttgccgaagc gacgcgccca tgaggcgccc ggcaaggtac gccaatcgac
     3421 gccatgccgc gatagagccc aaactcccca gatcgacgag ccgacgcaga tgacggcccc
     3481 tatctggaag cagctggaaa accccagtcc gaaaatctcc atggccaaaa aattgccgat
     3541 ggctttggcg gcgaactccc acagcttcca tttcacgacg ccagagcctt ggtcgagcgc
     3601 atggagcatg aaggccgaag tgctgctgac gaagcggaac ggcgcggcga acgaatacca
     3661 gagtactccg tcgcagatca gtccagcgat cgcatcgtca tcgacgagca gcctcgcaaa
     3721 tcggggatag ctcgccgagc cgaacgccgc gcagcacagg cctgcaaaca gcgagagcca
     3781 taggatcgcg ccgatgacgc gcggacttgc gccgcttcgc cgcgcctcgc cgatcgcggt
     3841 cgaagcgacg gcgcccagcg cgctcagcaa gatcacatcg acgacgacca gacgtgtcgt
     3901 catcgcataa gcgcccggcg cgccgccgcc caaatggccg accattatca catcggcttg
     3961 ctgtgcgagc tgccccgcca cgatcacgag aagcaggggc caatagcctc gccaaaagcc
     4021 cttgtcgctc tcgatcatag aggtatgggc cgccatcccg ggaaatgatc gagctcgtag
     4081 cccggcgtcc gaaaacatcc aaaattgtct tcgggcgccg gaagctcgca ttcgaactcg
     4141 acctgttcga cttcgatgaa gcgcgtctct atgctgctga gcttccagcc ggcgacagcc
     4201 ttcgtcggtc cgatcagacg gctcgcgatg acgatcccgc aataatcgag atcacggtcg
     4261 cgtcccgacc accgcgacgc ggccgcggcc atgagcttca gagcgaggtc tgctcccacg
     4321 cgttcacgaa actccgattg cagcagccat tccgcggccc aaatatgata ggcccaatcg
     4381 aatgtgaggt tcttctcctg cgcccattgc atgaacggca ggaaaatcgc gcgcccgtca
     4441 gggcccgaaa gctccaacaa ttgcggaacg gcgtcgaaca gcacatgcca ataggcccga
     4501 tagctgatgt cgagacgaac atgacttcgt gactctcgcg cgaacgcgtc gagccgggaa
     4561 gggttcatca tgtcttgata gagatcggcg tcgattctag ccggcgcagg cgacaagaga
     4621 ctcattggtc gtgtcctcgg cgtgggggaa tatctcgcgc aaacgttcga gatcgatcgc
     4681 gatcgagtca tattcgatct cgaaatcgcg ctcatatgtg atcgtcgagc ccggcttgtc
     4741 gaatgccgcg cgcatggagc gaaggaactc caacgtgtcc ggcgccattt ccctatcatg
     4801 cgaatcgacg atgactcgcg ggtccagaaa cgaaggccca tatcccgcga catgaaaatg
     4861 tcgcgtcgac gagatgacat cgttccaagc gtccagcggc gcgccgcaat tgtgtcgggc
     4921 gaccaccgca ttggaggcgt cgaagaggat ccccgctcca gtttctcggc agagccttcg
     4981 aaaaaactcg ggctgctccc atccgccgtc catgatggat ggatagtttt cgagatggag
     5041 ctggacgccg agcatatcct gccattgctc cacgcgattc cgaacggggg cgtattgggc
     5101 gtaatcgatt tcgccgagat agaagagatt ccgtccgttc ggcgcaaagc gcagcagatg
     5161 gtcggacacg tatgccggct gaagcgcatc gatgagaata cggatctgct tggcgagctt
     5221 ctcgagcgtc tctctgtcat tctcgagaaa cttcgacagc atgatatgga acccgaccgg
     5281 acactcgaac gcgtccgcca gctctttgac cggaacattc aagaaattgt cgatgagcag
     5341 ctcgcaatag tcgatttgcc gctctctgag catgcgccga accatgtcca gcgtgccgtt
     5401 caaggtgaag ttgaatccga tttgcatctg gaaagctttc gacgatgtcc gcatggaagc
     5461 tggaaagccg tccccggccg tatgaaccgg ggacggcgct gccgaataaa atcagcaggt
     5521 gccgcaccac ggcgggttgc acgcgcacat ggcctgaacg cggccgatca cggggaggat
     5581 ttccttcttg acgactttga tggtcattgc tttctccatt cggcgtttga tctgtcgcca
     5641 cgccgtcgca accggcgacg tgacgactcg ctcttccgca acgccccagc ggaagacgat
     5701 tccgtgtcgc gtttcgctct ttatgtcgaa agagattcgc ggctctcgac gggcgacagt
     5761 gatgcgagcg cttcgaggag cgcatcgttt tccgacggcg tcccgacagt gatgcgcaaa
     5821 tgctttccga tccgagaggt cggaggaaac gctttgacga agatgcgaaa tctctctctc
     5881 aattcctccg cgacgctcgc gccagtccgc ggcgtcgcgc aaaagaggaa attgccttcc
     5941 gagcgaaaag gagaaaagcc cggcatcctc gccagttcct cgcgcaagcg ctcacgctcg
     6001 cgcttcacct ggtcgatgag cggttggaac agagcggttt cttcgagaaa gattcgcgcg
     6061 acgcatgcgg tcatcgcatt cacgcggtaa gggagcgagg ctcgttccaa ttcgtcaatg
     6121 agcggctccg atgcgacgag atatcccaat cggcaccccg cgagtccgat cttcgaaaat
     6181 gattgcgcga cgattacatt gtggcgatcg atcgcatccg tcgcatagga gacgtcggca
     6241 taggcggaat atgcttcgtc caggcacagc aggtcgctcg tggacaccgc gagccgctcg
     6301 aatatggcgc gatccagggc gcggccggtc ggattattgg gattgccgac ataaaatatt
     6361 tttgcgccgt ccaccgccgt cattttgtcg acgacctccg tcgacaaggc aaatgcgtcg
     6421 tcgagcggaa cagtgacgaa gcctttgtcg ttgagcgcgg cgaaatgccc gtagagcggg
     6481 aatgtcggcg tcggaacgac caccgcttcg aacgggcccc cgaaggcacg acaaacaaga
     6541 tcgagcattt ccgtcgagcc gacgccgaga agaatatttt tggcccgcac gccgatattc
     6601 gcagcgatcg cggcctcgag cgcatcgatc tccatcgcat ctggatagcg tcgaaagtcg
     6661 atcgtcgaaa tctctcgcgc gacacgcgcc aggaattcgt ccgggagagg aaaacgcatc
     6721 tcattggcgt cgagacaaag gccgtcgccc cgcggaatcg cgccgtacat cggcgtcgct
     6781 cccaagcgtt tcttggcttg cggccggggc atcgcagagg gcgctgtttg cgcgagaaca
     6841 tcgatcagga ccgacaaggc gcgtctcctg ttctaccgat ggtgaaagct atcgtggcca
     6901 tgcctgggct cgctctgtgt gcttcatcga gacatgggac gatggcgcct cgcgcatctg
     6961 cgagtcattc acgaaacatg tcggatcatt cactcgtcat gagagacgca ccgatgcgat
     7021 cgaaaccgac atgggcgaaa aaaaaatttc tcgtgatacc gaaaaattcg ctcgatcgat
     7081 ctatgtcgcg gcgatcgact agaaagagac atacgtcgac gccggaactt atgcgcgaac
     7141 ctcgaacgaa cgcccgctcg agccgcagat gtggaagcga acggcctcac agcagtgacg
     7201 ccgttcgtca tgtttcttat cgggcggtcg tgtcggggag gctcagctcc agcgctttcg
     7261 acaatcgtgc gcgatgcgcc tcgatcttat cgctttcgga gcgaggaatt tgcggaaatg
     7321 cgagcgcctg cgcgcaagca tcggtcgcgg cggcgatcga tgtcggcgaa ttcggctttt
     7381 tgaagcacct gcgccagctc tcgatatatc tcgccatgcc cggatcgaag ggtttcgccg
     7441 atccggcgcg atgcgcatcc gcgtcggcgc cctcgtcatt ttcgcagact gtttcgatga
     7501 tcttgcaggg acctttcgca cggcattcgg cgtcggcttt gtctcgtgca tgagcgaggc
     7561 tgtcgccgag cgccgctacg tagcgagatc cgtcgagact cgccgcgacg gcggcgcagc
     7621 cgtcgtgaaa cgactgcgcg accgtgcagg cgcttctctc gccgccgcag acataaacgg
     7681 cgaatcgcgc ggccccgtgc gggtctgaaa agccgcgcgc ggagcccgtc agccattccc
     7741 cattgtcgcg tcgcccgagc gcgagagccg actccgcccg agagcccgaa gaggctcccg
     7801 atatgagcaa aatccacgtc gataggacgt atgcggcgcc gcgcccgagg cgcattctct
     7861 tcggcctgtg ggcgtgagcg tgaaatagtg agattggttc agtcatgaac gagctcgatt
     7921 ttcgatgagt cggcgaatgc gcgcgcctgc cgaaaagtca tgcaggattc gacgattgaa
     7981 acgccatgct atcgcgtaaa tcgccacggg ctttggtcaa atgcctttcc accgttcgtg
     8041 tcgatacgcc gcaacggtcg gcgatttcga tgttggtggc gccgtcgacc actttcaaga
     8101 ggatgatctg cttgcgcaat ggcgacagct gttcgaatgt cgaggaatag tcgtgaggct
     8161 cggcgacatt cggcgtgtcg gaatgaacgg attcgtcgaa gggcgtcgcg gcgtcggtaa
     8221 aggcggagcg gagctttcgc cgcctgtgca tgtcggtgac gagattggcg gtgatgcgat
     8281 atagataggc gcgcggacag gttagctttt cggctccttc cttcaccagt cgcagatagg
     8341 cctcctgaac gatgtcttca gattcttccc aaccgagtcg acggctggcg aattgacgta
     8401 atctgggcgc atagtcccgg tagagctgtt ggaccgactt ctccttcgct gtcat
//
