LOCUS       BGC0002133             11163 bp    DNA     linear   CON 01-MAY-2023
DEFINITION  Methylosinus sp. LW3 METLW3DRAFT_contig2.2_C, whole genome shotgun
            sequence.
ACCESSION   BGC0002133
VERSION     BGC0002133
KEYWORDS    .
SOURCE      Methylosinus sp. LW3
  ORGANISM  Methylosinus sp. LW3
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Methylocystaceae; Methylosinus.
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            AZUO01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: not full length.
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 17:00:20
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 11163
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..11163
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[6774:8164]"
                     /cutoff="5000"
                     /detection_rule="(DUF692 and MbnC)"
                     /neighbourhood="10000"
                     /product="methanobactin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      6775..8164
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(DUF692 and MbnC)"
                     /neighbourhood="10000"
                     /product="methanobactin"
                     /protocluster_number="1"
     cand_cluster    1..11163
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(DUF692 and MbnC)"
                     /kind="single"
                     /product="methanobactin"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..11163
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002133"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11163
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="methanobactin"
                     /region_number="1"
                     /rules="(DUF692 and MbnC)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            358..807
                     /locus_tag="METLW3_RS26010"
     CDS             358..807
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="METLW3_RS26010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_134188209.1"
                     /transl_table=11
                     /translation="MTNDKGRAYKSRMMNWGAQRKLSASAIIGALICFVALQSSFAYAR
                     NVEALRSHTSAAFESGNYEICRLASASDPKAPIQDHHTADQCCLLCASGGRDDQPTLLF
                     FLGVVSDFLAPLTSTDVADTCVDDIDRHLTTWTSSHSSRAPPRLS"
     gene            915..1415
                     /locus_tag="METLW3_RS0120530"
     CDS             915..1415
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0016987 - sigma factor activity [Evidence IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs)
                     SMCOG1126:extracytoplasmic-function sigma-70 factor (Score:
                     109.4; E-value: 2.9e-33)"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268284.1"
                     /locus_tag="METLW3_RS0120530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_024881679.1"
                     /transl_table=11
                     /translation="MFRTNGRSIGELFERNKRDLLDCLTRRVGYEDAFDLLQETFVRML
                     RRREVGPIADEDAYLRTTAINLARDFSRHSKTMAKHVAPGDIPAEIAETGLDPSQVCEA
                     KESARLLYAAMKGLPPKCREVFILRRFHDLSPDEIAQRLGISRNMVLKHLRLALERCHA
                     ALD"
     gene            1481..2470
                     /locus_tag="METLW3_RS0120535"
     CDS             1481..2470
                     /GO_function="GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]; GO:0004888 - transmembrane signaling
                     receptor activity [Evidence IEA]"
                     /GO_process="GO:0006811 - monoatomic ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268283.1"
                     /locus_tag="METLW3_RS0120535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FecR family protein"
                     /protein_id="WP_245267459.1"
                     /transl_table=11
                     /translation="MAAITPYKRQIGAEMEEDDAPRDRANRRRAAIEWWTRLDSRAPTP
                     QEREAFVIWLTHDPANREAFEKVCRIWGDLEDLRPLIDAFEVLPPRRTRRVRVAAVLAG
                     LAITLLSYVFFDDAWIMLRAQTRTGVAETRTIRLVDGSRIELGPRSAISLDFDEGKRNV
                     TLLKGEAWFDVAPDAGRPFSVLVARGSVTALGTSFDISTTGERTEITVAQHRVRVMTGG
                     PAIIVDEGEQSAFGPGVAAVDPYRVQVDHVAAWRRGKLIFDDKPLAEVVSVLGHYLSGY
                     ILILDPSIRERRVSGVFDAADPIAAISAIEKALGLRALDLGYLVVLTG"
     gene            2629..5136
                     /locus_tag="METLW3_RS0120540"
     CDS             2629..5136
                     /GO_function="GO:0015267 - channel activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs)
                     SMCOG1051:TonB-dependent siderophore receptor (Score:
                     541.8; E-value: 4.4e-164)"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003615874.1"
                     /locus_tag="METLW3_RS0120540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TonB-dependent receptor"
                     /protein_id="WP_024881681.1"
                     /transl_table=11
                     /translation="MGATAILTAMGALAFGSNADRAEAHAQMPQKEIAEIVRSYDIPAG
                     PISTALNSVADASGVRIVYDSRLTRSYRTRGVSGSHSLAAALSEVLSGTGLSYELSPNG
                     KSVLIVLAQAGDTRNDASAGGATPLPTIDIGSEPNRPTSRPGSGAGSQGPGDRHTGYNA
                     VRAPSTLKTDTPLLKTPITVEVVTRQTMDDQQAISVNDALLSNVSGVTQGISGIELFKV
                     RGFYNTVGNVYKNGLMEYRLRNLDTTNLQAIEVLKGPAAMLFGRGEPGGIINLVVKRPL
                     ETPYYSLQQQVKSFGGTRTTLDATGPLNDEKSVLYRFNGEFYSTDSYRNFVTDRNLFIA
                     PTITIRPVEQFKMNIDFEYQNKTYVDDYPTFPAIGGAPANIPVSRYLGEPSIMKSTPNH
                     FERKRIAYDATYEFLPGWSLTNRLSYYHIDTNNENLSILGINQATGVMNRVVNFLPGYG
                     EDSFGANVDLKGRFTTGPIEHSVLFGYDYFGTYLPVNITYFFSPISTINIYAPTYWLPE
                     NPYRGTASYGAAGQKWTGVYGQDMLSFFDDSVHVLLGGRYDWTETGSNKIGTSDYAARA
                     SYVNVYSNAFSPRVGVVYQPLPWLSLFGDFTQSFGANNGTTQNLAPLAPQKGEQYEAGI
                     KAELLDKRLSLTMAYFDIVKSNVPYTDPTNTRNTLLIGKARSQGFEFDLTGRIDDNWSV
                     IANYTHDDVRTVEGSPSYNPLTLITTQLAVTGKKLAGSPRNYGNLWIKYDADGAFRGLG
                     LGAGVSVSESYLGDNANSFVVPGYAIVNGMISYTTQIAGLTVTGQLNVKNIGDARYFPA
                     SYDRYTIQTGAPRTFLGSLRVEF"
     gene            5200..6177
                     /locus_tag="METLW3_RS0120545"
     CDS             5200..6177
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921436.1"
                     /locus_tag="METLW3_RS0120545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="metallo-mystery pair system four-Cys motif
                     protein"
                     /protein_id="WP_024881682.1"
                     /transl_table=11
                     /translation="MMREQKSKLAALLMAALTVGTCHGAAARDQAVTLRFALTADGKDV
                     GCAAPLSNLGSGRLETKLHDARFYIHDVKLIDAKGVRTPVALAQNDWQYAGVALLDFKD
                     ARGGNTPCTDTNPAKNTTVGGTAPAGAYAGLEFSIGVPVQGMADDKIVSLNHSSTETAA
                     PPLDIAAMAWNWQAGRKFLLVEVIPPAPYITKSDGSKTRIWMTHLGSSGCKGNPATGEI
                     VSCARENRFTVTFDRFDPKTQRVEFDLTRLFENTDLLVDKGGAIGCMTALDDPECPAIF
                     SALGLNLAESAPGAGDAGKQTKPGVSPVFKVGAAKPAKIVGGKQ"
     gene            6597..6695
                     /gene="mbnA"
                     /locus_tag="METLW3_RS28905"
     CDS             6597..6695
                     /codon_start=1
                     /gene="mbnA"
                     /inference="COORDINATES: protein motif:HMM:TIGR04071.2"
                     /locus_tag="METLW3_RS28905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="methanobactin"
                     /protein_id="WP_161912153.1"
                     /transl_table=11
                     /translation="MAIKIAKKEVLPVVGRLGAMCSSCPMCGPLCP"
     gene            6775..7608
                     /gene="mbnB"
                     /locus_tag="METLW3_RS0120550"
     CDS             6775..7608
                     /codon_start=1
                     /gene="mbnB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     methanobactin: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268275.1"
                     /locus_tag="METLW3_RS0120550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="methanobactin biosynthesis cassette protein MbnB"
                     /protein_id="WP_024881683.1"
                     /sec_met_domain="DUF692 (E-value: 1.1e-21, bitscore: 66.5,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLIGFNFTLNATLDMVQRMIKARQIDYCELLIDNFLHVPPKELET
                     TLDCPIAFHIMFSKFLESDDEVLGKLAKRIRILIDALNPIYVSDHLLRFTHEGRCFYNL
                     GEIDYETQFDAVRRRVEKWQDLLGRRLYLENFPSIMEGGWDAPAFYRRLSKETGAGVLF
                     DASNAVCAKHNCGAPLDLWNDIVETTQHFHVAGYAQSFSAPHVIVDAHDREMAPDTIEF
                     LSSMRSSFDKPGATITYERDINIDYDSIAVDLQRLREIFPHTQELEHADLVACAS"
     gene            7580..8164
                     /gene="mbnC"
                     /locus_tag="METLW3_RS0120555"
     CDS             7580..8164
                     /codon_start=1
                     /gene="mbnC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     methanobactin: MbnC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614727.1"
                     /locus_tag="METLW3_RS0120555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="methanobactin biosynthesis cassette protein MbnC"
                     /protein_id="WP_024881684.1"
                     /sec_met_domain="MbnC (E-value: 7.4e-78, bitscore: 249.9,
                     seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPILSPAPARIDADLYADLIDPARLATYPRESRSYIRIDISLRAY
                     WHTLFDTCPLLLDLSGPDGASIFHPFMAWARQEKLSFDWTYFLWVYQWLAQSEFRDRLS
                     EELLLSMMTASAARWLGYDRNVDDLGVVIGTPLIDGVVVGWKMDSVYCGLNQVERLEFE
                     EPLPAPDGLFGYFFTPSLRLGHFPGWRPIPR"
     gene            8161..9441
                     /locus_tag="METLW3_RS0120560"
     CDS             8161..9441
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1086:MATE efflux
                     family protein (Score: 57.7; E-value: 1.5e-17)"
                     /gene_kind="transport"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="METLW3_RS0120560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_024881685.1"
                     /transl_table=11
                     /translation="MIESGFWRGYWPLLVSIGSTQLVLQVDIVTLGWLGGDAQSAYVLL
                     TRLALLDLVLMTAMGTVASTIVAQRRRTADGATAIGGALFLSAIGGLFCGLLGFMFYPR
                     LAALVAGEGEVAAQIETAIYLYTVATPLRFSVTAAILILHALDEGVSIVRWKLIEGALK
                     IGGNLLFIGVLGLGLRGCFASGLVVAIVSSIWCWRILSSRHAAPFHIPSCSLARSFFRS
                     TAWESQRTVAVHFAVTVGVMLFAAPWLGHYDISRLSAYAAGQTLMLLVFAPLLALTRFL
                     SFRLARVGDDVIAPLVRALWLRGAPVSFFAALVLFTSRDLLGDLYGQQGPWWSSLIVAL
                     AVSLPLRHAANVTRAALLSRGAFAEVAKADSVALWSCAVPLLALGLAADSPPLTYTSLI
                     VPEAICALWLWRRLTLPRSSVRANAAL"
     gene            9508..11163
                     /locus_tag="METLW3_RS0120565"
     CDS             9508..11163
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050:monooxygenase FAD-binding (Score: 362.2; E-value:
                     8.4e-110)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921441.1"
                     /locus_tag="METLW3_RS0120565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_024881686.1"
                     /transl_table=11
                     /translation="MPSEHAPVLIVGGGYAGLTAALMLSIRGVPCRLIERRAALSDHPR
                     AHGFNLRSLELLRQVPGLENDLRLVTRAAPGDTTIMVAETVTSSPIKIMATPGGFDPRP
                     LSPAMLCSAGQDRVEPVLLRHARAHGADIRFSTELIRVSQDVDNVRACLRDTESGEETM
                     VHADYLVAADGSSSAIRRALGVDMIGLDGVSHAVSILFQADLSIAMGKRGFLLCYLRHR
                     DFTGAFVSCDDPDRGQLNVEYDAARETVSDFDVERCKKLVRAALGQSDLDVQILNVLPW
                     RMSALLAERMILGRVFLAGDAAHIMPPVGGLAGQTAIQDAADLAWKLAMVIKGEARPAL
                     LDTYDAERRPVAQLSIARATENYVERLRQDRSDLSDAFGRVNYLDAAMSYRYRSPAISL
                     DEPDDGLPADDTLHPSGKPGSRLAHVPLVRNGAEISTHDLVGQGFVLLAGPSGSAWIEA
                     ARALSHGEDAPLRAFLIGVDVDDPTGAFLSRTGLGPSGALLVRPDAFIASRSVDGSADS
                     EAILRRSLDRARCLDRREVPPSSPAYASMAKVVP"
ORIGIN
        1 atgcgcctat ggtcgaagtc gaggcggtca ggcggaggag aagacgggcg ctcggcgcgg
       61 gtgtccgaag atgttcagaa agaccgaggg aaacaaggcg gcgatcgcga tctggccgaa
      121 tggaatcgcc cgcgtgtcga tcgtgggcct cttggctcgg tctctcttca tcgtcaggta
      181 aggcggcggc cgctttgtcc gatgccgtcg accgcggcat gtcgctgcga gcgtcagtca
      241 ttgcgtcggc gacgaccgtc gccatgcccc cggcggcatt ttcctgtccc tgccgcattc
      301 ctcgcgagtc cgctcggctc gacgatcgcg ctggaacttt ctcggtcctt cctaaacttg
      361 accaatgaca aggggcgggc ttacaaatca cgcatgatga actggggcgc acagcgaaaa
      421 ctatcggcga gcgccattat cggcgctttg atctgtttcg tcgcattgca gagcagtttt
      481 gcctatgcgc gcaatgtgga agcgctccgt tctcacacct ccgccgcttt cgagtctgga
      541 aattatgaga tttgcagact cgcctccgcg tcggatccaa aagcgccgat ccaggaccac
      601 cataccgccg atcaatgctg ccttctctgc gcatcgggtg gtcgtgacga tcagcctact
      661 ctgcttttct tcctcggcgt tgtctccgat tttctcgccc ctctgacctc gaccgacgtc
      721 gccgatacgt gcgtcgacga catcgaccgt caccttacga cctggacgag ttctcactct
      781 tcccgcgctc cgccgcgtct gtcctgaacg cttctcccgc tctggagcgg gaaaatcgtt
      841 cggggcgggg cccgctcggg cggcctctcg cttcgccgcc atcgcgagtt cgaggctcca
      901 gcggaagcca catcatgttc agaaccaatg gccgttcgat cggcgagctc ttcgagcgca
      961 acaagcgcga tcttctcgac tgtctcacgc gtcgcgtcgg ttacgaggac gctttcgatc
     1021 ttctacaaga gacctttgtg cggatgctgc ggcgtcggga agtcggaccg atcgccgacg
     1081 aggacgccta tctacgaacg acggcgatca atctcgcgcg cgacttctcc cgtcattcca
     1141 agaccatggc caagcacgtc gcgccaggcg acatcccggc cgagatcgcc gagaccggac
     1201 tcgatccgtc acaagtttgc gaggcgaagg aaagcgcccg cttgctgtac gccgcgatga
     1261 aaggcctgcc ccctaagtgt cgggaagtct tcatcttacg tcgcttccat gatctttcgc
     1321 cggacgaaat agctcaacgg ctgggcattt cccgaaacat ggtcctcaag catctgcggc
     1381 tggcgctgga gaggtgccat gcggcgctcg actaaatttt cacatcgaga cgcccacctt
     1441 atatcactgt cggcatccgt cctgctaaag tagtggatca atggcggcga taacgcccta
     1501 taagaggcag attggcgcgg agatggagga ggatgacgct ccccgtgatc gggcgaaccg
     1561 tcggagagcg gccatcgaat ggtggacgcg cctggacagt cgcgctccga ctccgcagga
     1621 gcgcgaggcg ttcgtcattt ggctgacgca tgatccggcc aaccgcgagg cattcgagaa
     1681 ggtttgccgt atctggggcg atctcgaaga cctgcggccg ctcatagacg ccttcgaagt
     1741 gcttccgccg cgccgaacgc ggcgcgttcg cgtcgccgcc gtcctcgcgg ggctcgccat
     1801 aacgctgttg tcctatgtct ttttcgacga cgcctggatc atgttgaggg cgcagacccg
     1861 caccggcgtc gcggagacgc ggaccattcg gctcgtcgac ggatcgcgga tcgagctcgg
     1921 ccctcgctcg gccatctcgc tcgatttcga cgaaggcaag cgcaatgtga ccctcctgaa
     1981 gggcgaggct tggttcgacg tcgcgccgga cgccggcagg cccttttccg ttctggtcgc
     2041 ccgcggctcg gtgacggctc tcggcacgag cttcgacatc tcgacgacgg gggagcgaac
     2101 ggagatcact gtcgcgcagc atcgggtgcg cgtcatgacg ggtggcccgg cgatcatcgt
     2161 cgatgaaggc gagcagagcg cttttgggcc gggcgtcgcc gcggtcgatc cataccgggt
     2221 gcaagtcgac catgtcgccg catggcgtcg cggaaagctg atattcgacg acaagccgct
     2281 cgccgaggtc gtctctgtgc tcggacatta tctgagcgga tatattctca ttctcgatcc
     2341 atcgatccga gaaaggcgcg tgagcggcgt gttcgacgcg gcagatccga tcgccgccat
     2401 ctccgcgatc gagaaggctc tcggccttcg cgctctcgat ctcggctatc tcgtcgtgct
     2461 cacgggataa gagaggattt tttttgctcg tcgacctccc cgcgggaggg gtcggttcgt
     2521 ccttgtctga gtgggaggcg tggtgtctgc cgccaggaga cgggacagga ccaaaacgag
     2581 atgtcggatc ggattcctca cggcagcaag tcagcgaagg gtgggatgat gggggcgacg
     2641 gccatcctga ccgcgatggg cgctctcgca ttcggatcga acgcggatcg cgccgaagcg
     2701 catgcgcaga tgccgcagaa ggaaatcgcc gagatcgtac gaagctacga cattccggcc
     2761 ggccccatct cgactgcgct caacagcgtc gctgacgcga gcggcgtgcg catcgtctat
     2821 gactcacgct tgacccgctc gtatcggacg agaggcgtct cgggatcgca tagtctcgcg
     2881 gcggcgctgt cggaagtcct gtcgggaacg gggctgagct acgagctgtc tcccaacggc
     2941 aagtcggtgc tgatcgtcct ggctcaagcc ggcgacactc gcaacgatgc gagcgctggc
     3001 ggcgccacgc ctctgccgac gatcgatatc ggctccgagc ccaatcgtcc gacaagccgc
     3061 cccggctcgg gcgcaggcag ccaagggccg ggagatcggc acaccggcta caacgccgtc
     3121 cgcgcgccct cgacgctgaa gacggacacg ccgctactga agactccgat cacggtcgag
     3181 gtggtgacgc gccagacgat ggacgatcag caggcgatct ccgtcaacga cgccttgctc
     3241 tccaatgtca gcggcgtgac gcaagggatc agcggcatcg agctgttcaa ggttcgcggt
     3301 ttctacaaca ccgtcggcaa cgtctacaag aacgggctga tggaatatag gctgcgcaat
     3361 ctcgacacga cgaatctgca agcgatcgaa gtgctgaaag gtccggccgc catgctgttc
     3421 ggcagaggcg agcccggcgg catcatcaat ctggtcgtca agcgaccgct cgagacgcct
     3481 tattattcat tgcagcaaca ggtgaaatcc ttcggcggga cacgaacgac tctcgacgct
     3541 actgggccgc tgaacgacga gaagagcgtt ctctaccggt tcaacgggga gttctacagc
     3601 accgattcct accgcaattt cgtgaccgat cgaaatcttt tcatcgcgcc gacgatcacc
     3661 attcgtccgg tcgagcagtt caagatgaac atcgatttcg aatatcagaa caagacctat
     3721 gtcgacgatt atccgacctt ccctgcgatc ggcggcgctc cggccaatat tccggtgagc
     3781 cgatatttgg gcgagccgtc cattatgaaa tcgacgccga accacttcga gcgcaagcgc
     3841 atcgcctatg atgcgactta cgaattcctt cccggctgga gcctgacgaa ccggctcagc
     3901 tattaccaca tcgacacgaa caacgagaat ctctcgatac tgggcatcaa tcaggcgacg
     3961 ggagtgatga accgtgtcgt gaatttcctg cccggctatg gcgaggacag cttcggcgcc
     4021 aatgttgatt tgaagggccg tttcacgacc gggccgatcg agcattccgt gctgttcgga
     4081 tatgattatt tcgggaccta cctgcccgtg aacatcacct atttcttcag cccgatcagc
     4141 acgatcaata tttatgcgcc cacttattgg ctgccggaga atccctatcg agggaccgcc
     4201 agctacggcg ccgcgggtca gaaatggacc ggcgtctacg gccaggacat gctctccttc
     4261 ttcgacgatt cggtacatgt gctgctcggc ggccgctacg actggacgga aacgggctcg
     4321 aacaagatcg gcacgtccga ctatgccgcg cgcgcctctt atgtgaatgt ctactccaac
     4381 gccttcagcc cgcgcgtcgg agtcgtctac cagccgcttc cctggctttc gctgttcggc
     4441 gatttcacgc agtccttcgg cgcgaacaac ggcacgacgc aaaatctcgc gccactggct
     4501 ccgcagaaag gcgagcaata tgaagcaggc atcaaggcgg agctcctcga caagcgcctc
     4561 tcgctgacga tggcctattt cgacatcgtc aagtccaacg tcccctatac cgatccgacg
     4621 aacacgcgaa acacgctgct gatcggcaag gcccgcagcc agggcttcga attcgatctt
     4681 acgggccgca tcgacgacaa ttggagcgtg atcgccaatt atacgcatga cgatgtgcgc
     4741 accgtggagg gatcgccctc ctataatccg ctcaccctca tcacgaccca gctcgccgtc
     4801 accggaaaga agctggcggg aagtccgcgc aattacggaa atctctggat caaatatgac
     4861 gcggacggcg ctttccgcgg ccttggcctc ggagccggcg tgtcggtctc ggagagctat
     4921 ctcggcgaca atgcgaattc cttcgtcgtg ccgggctatg cgatcgtcaa tggcatgatt
     4981 tcctacacga cgcagatcgc cggcctcacc gtcacgggtc agctgaatgt gaagaatatc
     5041 ggcgatgcga gatattttcc ggcctcttac gaccgctaca ccatccagac cggcgcgccg
     5101 cgcactttcc tcggctctct gcgcgtggag ttctaagtcc gtttgcgaat tcggcggccg
     5161 actgaaacga gggtgcgatc cagctggtag gagcgacgaa tgatgcggga acaaaagtcg
     5221 aagctcgcgg cgctgctgat ggcagctctc acagtcggaa cctgtcacgg cgccgcagcg
     5281 cgggaccagg cggtgacgct ccgcttcgcg ctgacggcgg atggtaagga tgtcggttgc
     5341 gctgcgcctc tgtccaatct cggtagcggg cgtctcgaga cgaagctgca cgacgcccgc
     5401 ttctatattc acgacgtgaa gctgatcgac gcgaagggcg tgcgcacgcc cgtcgctctc
     5461 gcgcagaacg actggcaata tgccggcgtc gctctgctcg acttcaagga cgcgcgcggc
     5521 ggcaacacgc cttgcaccga caccaatccc gcaaagaaca cgacggtcgg cggaaccgcg
     5581 ccagctggcg cctatgcagg actcgagttc tcgatcggcg tgccggtcca gggcatggcg
     5641 gacgacaaga tcgtctcgct caatcattcg agcacggaaa ccgcggcccc gccgctcgac
     5701 atcgccgcca tggcgtggaa ctggcaggcc ggccgcaaat ttcttctcgt ggaagtcatc
     5761 ccgcccgcgc cgtacatcac caagtccgac ggctcgaaga cgcggatatg gatgacgcat
     5821 ctcggctcgt cgggctgcaa gggcaatccg gcgacgggcg agatcgtctc ctgcgcgcgc
     5881 gagaatcgct tcaccgtcac cttcgaccga ttcgatccca agacccaacg cgtcgagttc
     5941 gatctgacgc gcctgttcga gaacaccgat cttcttgtcg acaaaggtgg cgcgatcggc
     6001 tgcatgaccg cgctggacga tccagaatgc ccggcgatct tctcggcgct cggtctcaat
     6061 ctcgcggaaa gcgctccagg cgctggcgac gccggcaagc agacgaagcc cggcgtctcg
     6121 ccggtcttca aggtcggcgc cgccaagccg gcgaagatcg tgggcggcaa gcaatgaaac
     6181 gcgcaccgct tcgctcgcgt tcgtagccga tatcgccgca gcggcgaccg cgccgcaaca
     6241 ttgtggcgcg gtcgccctgc atgcatgacg ggacgaccgc gacgctcgaa gagttcatcg
     6301 ataatcacgc gcagcgctgc cgctggatcg atctctcagc gcgcgtctcc ggcccattgg
     6361 aggcctgcgc cacaaaatgt atttgtgcaa tctatactac gtacgcaatg tatatatttt
     6421 ttacactcag ctattttcgg ccaacggcga gaaaaatatg cggttttgta ttgcaattgg
     6481 agaattttca gccattatat atttgtcgca tggctgaacg ccgcagatgc gatgctttgt
     6541 ctattctgta taatacagtc agactggctc aactcggcgt ttttcaggag gaagcaatgg
     6601 ctatcaagat cgccaagaag gaagttctcc cggtcgtcgg tcgtctgggc gcgatgtgca
     6661 gctcgtgccc gatgtgcggc ccgctctgcc cctgatcggc aggccgcccc cgtcgcattc
     6721 gccgggggcg gcgtctcgag acctacggtc attttcttgg aggcctgttt cgatatgctg
     6781 atcggcttca atttcacgct caacgcgacc ctcgacatgg tccagcggat gatcaaggcg
     6841 cgccagatcg actattgtga gctattgatc gacaattttc tccatgtccc cccaaaagag
     6901 ctggagacga ctctcgattg cccgatcgca tttcatatca tgttctcgaa atttctcgag
     6961 agtgacgacg aggtcctcgg caagctggcg aagcggatac ggattctcat cgacgcattg
     7021 aacccgatct acgtgtcgga ccatttgcta cgttttacac atgaaggcag atgcttctac
     7081 aacctgggcg aaatagatta cgagacgcaa ttcgacgccg ttcgacggcg cgtcgagaag
     7141 tggcaggatt tgctcggacg tcgcctctat ctcgaaaatt ttccatcgat catggagggc
     7201 ggatgggatg cgccggcctt ttatcggcgc ctatccaagg agaccggcgc gggagttctc
     7261 ttcgacgcat cgaacgccgt ctgcgcgaag cacaattgcg gcgcgccgct cgacctctgg
     7321 aacgatatcg tcgagacgac gcagcatttt catgtcgcgg gctatgcgca gtcattcagc
     7381 gcgcctcatg tcatcgtcga cgcgcatgat cgagagatgg cgccggatac gatcgaattt
     7441 ctgtcctcga tgcgctcgtc attcgacaag cccggcgcga cgatcaccta cgagcgcgac
     7501 atcaacatcg actatgattc gattgcagtc gatctgcagc gtctgcgcga gatattcccc
     7561 cacactcagg agctcgagca tgccgatctt gtcgcctgcg ccagctagaa tcgacgccga
     7621 tctctatgcc gatcttatcg accctgcgcg gctcgcgacc tatccacgcg agtcacgttc
     7681 ctatatccgc atcgacataa gccttcgcgc ctattggcac acgctgttcg acacctgccc
     7741 attgctgctc gatctgtccg gcccggacgg agcgtcgatc ttccatcctt tcatggcctg
     7801 ggccagacag gagaagctgt ccttcgattg gacctatttt ctgtgggtct atcaatggct
     7861 ggcgcagtcg gagtttcggg accggctgag cgaggaacta ttgttgtcga tgatgacggc
     7921 ctccgccgct cgctggctcg gctatgatcg caatgtcgat gacctcggcg tcgtgatcgg
     7981 cacgcctctg atcgatggag tggtggtcgg ctggaagatg gacagcgtct attgcggcct
     8041 gaaccaggtg gagcgcctcg agttcgagga gccgcttccg gcgccggacg gcctgttcgg
     8101 ctatttcttc acgccaagtc tcagactcgg ccattttccc ggctggcggc cgataccgcg
     8161 atgatcgaaa gcggcttctg gcgcggatat tggccattgc tcgtcagtat cggctccacg
     8221 cagctcgtgc tgcaggtgga tatcgtcacg ctggggtggc tgggaggcga cgcgcagagc
     8281 gcctatgttc ttctcacgcg tctggcgctg ctcgatctcg tgctgatgac agccatggga
     8341 acggtcgcct cgaccatcgt cgcgcaaagg cgacgcacag ccgacggggc gacagcgatc
     8401 ggcggcgcgc tgttcctctc ggcgatcggc ggcctgttct gcgggcttct cggcttcatg
     8461 ttctatccca gattggctgc gttggtggca ggcgagggcg aagtcgcggc gcagatcgag
     8521 acggctatct acctgtacac ggtcgccacg cccctgcgct tctccgtcac cgccgccatt
     8581 ctcattctcc atgcgctgga cgaaggcgtt tccatcgttc gctggaaatt gatcgaaggc
     8641 gcattgaaga tcggtggcaa tctcctcttc atcggcgttc tgggcttggg gcttcggggc
     8701 tgcttcgcga gcgggctcgt cgttgcgatc gtctcgtcca tatggtgttg gcgcatcctc
     8761 tcttcgcgtc acgcagcgcc gttccacatt ccgagctgtt ccttggctag gagtttcttt
     8821 cgttcgacgg cgtgggagtc gcagcgaact gtcgcggtgc atttcgcggt aacggtcggc
     8881 gtcatgcttt tcgcggcccc ctggctcgga cattacgaca tttctcggct gagcgcctat
     8941 gccgccggac agacgctgat gctccttgtc ttcgcgccat tgctggcgct cacgcgcttt
     9001 ctctctttcc gactggcgcg cgtcggggac gacgtcatcg cgccgctcgt ccgcgcgctt
     9061 tggcttcgcg gcgcgcccgt ctctttcttc gctgcgctcg tgctgttcac aagccgcgat
     9121 ctgctcggcg atctgtacgg tcagcagggg ccttggtggt cgtccctgat cgtggcgctc
     9181 gccgtttcac taccgcttcg acacgcggcc aatgtgacga gggccgcgct gctgtcgcgc
     9241 ggagctttcg ccgaggtcgc gaaagcggac agcgtcgcgc tctggtcctg cgccgtgcct
     9301 cttttggcgt tgggactcgc ggccgattcg ccgccgttga cctatacgtc gctgatcgtc
     9361 cctgaggcta tctgcgcgct gtggctctgg cggcgtctca cactgcccag gagcagcgtg
     9421 cgcgcgaacg cggccctctg aaaagccatg atccgcgagg ggcgccgaga gaagacgcga
     9481 cgcgagagag caggaggttg caggaccatg ccatccgaac atgcgccagt tttgatcgtg
     9541 ggcggcggat atgcggggct gacggccgcg ctgatgcttt ccatccgcgg cgttccgtgc
     9601 cggctcatcg agcgccgcgc cgcgctctcc gatcatccca gagcgcacgg gttcaatctt
     9661 cgcagcctcg agctgctacg gcaggttcct ggcctcgaaa acgacctgcg cctcgtcacg
     9721 cgagctgcgc ccggcgacac gacgatcatg gtcgctgaga cggtcacgag ctcgcccata
     9781 aagataatgg cgacgccggg cggattcgat ccgcgcccgc tctcgccggc aatgctttgc
     9841 agcgccggcc aggatcgcgt cgagccggtg ctgcttcgcc atgcgcgagc gcatggcgcc
     9901 gacattcgct tttcgaccga gctcatccgt gtatcgcaag acgtggacaa tgtccgcgcc
     9961 tgtctgcgcg acacggagag tggagaggag acgatggtcc atgccgacta tctcgtcgcc
    10021 gccgatggaa gcagcagcgc tatccgcaga gcgctcggcg tcgacatgat cggactcgac
    10081 ggcgtgtctc acgcagtgtc cattctcttc caagccgatc tgtcgatcgc gatggggaag
    10141 cgcggcttcc ttctctgcta cttacgtcat cgcgacttca cgggagcctt cgtcagctgc
    10201 gacgatcccg accgtgggca actcaatgtc gaatatgacg cggctcgcga aaccgtgtcg
    10261 gacttcgatg tcgagcgatg taagaaactc gtgcgcgcgg cgttgggtca atccgatctc
    10321 gacgtccaga ttctgaacgt ccttccctgg cggatgtcgg cgttgctcgc cgaacgaatg
    10381 atcctcggcc gtgtgtttct cgccggcgac gcggcccata taatgccgcc ggtcggaggc
    10441 ctcgccggtc aaaccgcgat ccaggatgcg gccgatctcg cctggaagct cgccatggtg
    10501 atcaaaggcg aggcccgccc ggctttgctg gacacctatg acgccgagcg ccgtcccgtc
    10561 gcgcagctct cgatcgcgcg tgcgaccgag aattatgtcg aacggctacg tcaggaccgt
    10621 tccgatctct ctgacgcctt cggtcgagtc aactatctcg acgccgcgat gagctatcgc
    10681 tatcgatcgc cggctatttc cttggacgag ccggacgatg gactgccggc ggacgatacg
    10741 ctgcatccga gcggaaagcc cgggtcccga ctcgcgcatg ttccgctcgt cagaaacggc
    10801 gccgagattt cgacgcacga tctcgtggga caaggatttg tgctgctggc cggtccctcc
    10861 ggatccgcat ggatcgaggc cgcgcgagcg ctctctcacg gcgaagacgc gccgttgaga
    10921 gcctttctga tcggagtgga tgtcgatgat ccgaccggtg catttctctc gcgaaccggg
    10981 ctcggaccga gcggagctct gctcgtgcgc cccgacgcct tcatcgcatc gcgatcagtc
    11041 gatgggagcg cagattccga agcaattctg aggcgttccc tcgatcgcgc gcgctgtctc
    11101 gatcggcgcg aagtcccacc ttcgtcgcct gcttacgcgt cgatggccaa agtcgtgccg
    11161 tga
//
