BGC0002134: leupeptin A biosynthetic gene cluster from Xenorhabdus bovienii SS-2004
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Location: 578,227 - 604,000 nt. (total: 25,774 nt).
This entry is originally from NCBI GenBank FN667741.1.

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
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General information about the BGC

MIBiG accession | BGC0002134
Short description | leupeptin A biosynthetic gene cluster from Xenorhabdus bovienii SS-2004
Status | Quality: questionable The quality level of this entry. Status: active The status of this entry. Completeness: complete Whether the entry covers everything needed for the pathway producing the compound(s)

Remarks "This pathway is non-NRP, using separate AMP-dependent ligases to form amide bonds, with a reductase to form aldehyde."
Biosynthetic class(es)

- other (other)

Loci
FN667741.1
588227 - 594000
via Heterologous expression
Compounds

- leupeptin A
- leupeptin B
- leupeptin D
- leupeptin C

Species Xenorhabdus bovienii SS-2004 [taxonomy]
References

- [1] Making and Breaking Leupeptin Protease Inhibitors in Pathogenic Gammaproteobacteria. Li, J et al., Angewandte Chemie International Edition (2020) DOI:10.1002/anie.202005506
- [2] Making and Breaking Leupeptin Protease Inhibitors in Pathogenic Gammaproteobacteria. Li JH et al., Angew Chem Int Ed Engl (2020) PMID:32609431

Chemical products information
leupeptin A Evidence: Mass spectrometry, MS/MS
Copy SMILES
C20H38N6O4
Chemical database entries
NPAtlas
leupeptin B Evidence: NMR, Mass spectrometry, Chemical derivatisation
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C23H35N3O5
leupeptin D Evidence: Mass spectrometry, Chemical derivatisation
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C19H35N3O4S1
leupeptin C Evidence: NMR, Mass spectrometry, Chemical derivatisation, Total synthesis
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C23H35N3O4
List of genes involved in compound(s) production

Identifiers | Position | Product | Functions | Evidence | Extra
--- | --- | --- | --- | --- | ---
XBJ1_0572 CBJ79716.1 | 588227 - 589132 (-) | acetylation | Mutation phenotype: <mibig.converters.shared.mibig.gene.function.MutationPhenotype object at 0x7f970b2a1310> |  | copy AA seq copy Nt seq
XBJ1_0573 CBJ79717.1 | 589147 - 590418 (-) | activate carboxylate with ATP and ligate second amino acid | Mutation phenotype: <mibig.converters.shared.mibig.gene.function.MutationPhenotype object at 0x7f970b2a1150> |  | copy AA seq copy Nt seq
XBJ1_0574 CBJ79718.1 | 590463 - 592868 (-) | ligate third amino acid, reduce CoA thioester to aldehyde | Mutation phenotype: <mibig.converters.shared.mibig.gene.function.MutationPhenotype object at 0x7f970b2a0b10> |  | copy AA seq copy Nt seq
XBJ1_0575 CBJ79719.1 | 592870 - 594000 (-) | activate carboxylate with CoA | Mutation phenotype: <mibig.converters.shared.mibig.gene.function.MutationPhenotype object at 0x7f970b29ffd0> |  | copy AA seq copy Nt seq

Biosynthesis information

No biosynthesis information available for this record.

Annotation changelog

#### Entry version: 3

Date
Changes
Submitters
Reviewers
2023-12-04
Fix compound evidence

- (ID: 3UOU7PODQJXIM6BEPUHHRRA5)

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#### Entry version: 2

Date
Changes
Submitters
Reviewers
2022-10-07
Update chemical activity to schema version 2.11

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

2022-10-07
Changed chemical structures for compounds: leupeptin A.

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

2022-10-07
Added NPAtlas links to compounds: leupeptin A.

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

#### Entry version: 1

Date
Changes
Submitters
Reviewers
2022-09-15
Submitted

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

2022-09-15
Added AMP-binding domain substrate specificities

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