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Overview
1.1

### Identified secondary metabolite regions using strictness 'relaxed'

BGC0002141 (Streptomyces bottropensis)

Region | Type | From | To | Similarity Confidence | Most similar known cluster
--- | --- | --- | --- | --- | ---
Region 1 | T2PKS,PKS-like,betalactone,oligosaccharide | 1 | 79,353 | High | trioxacarcin A | PKS

BGC0002141 - Region 1 - PKS-like,T2PKS,betalactone,oligosaccharide
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
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Location: 1 - 79,353 nt. (total: 79,353 nt) Show pHMM detection rules used
Region on contig edge.
T2PKS: (t2ks and t2clf)
PKS-like: (t2pks2 or ksIII)
betalactone: (HMGL-like and AMP-binding)
oligosaccharide: (repeatable(3, [DUF1205, Glyco_transf_28, Glycos_transf_1, Glycos_transf_2, MGT]) and minscore(MGT, 150))

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
MIBiG
Tailoring
MIBiG comparison
KnownClusterBlast
NRPS/PKS domains
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
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Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
External annotation
This annotation was made externally by 'MIBiG' and not by antiSMASH

Name |  | MIBiG
Version |  | 4.0alpha

Type |  | Subregion
Location |  | 1 - 79,354
Label |  | BGC0002141

Tailoring functions
Tailoring enzymes detected in this region
Oxidoreductases: dehydrogenase oxidoreductase oxygenase
AKT74271.1: short-chain dehydrogenase/reductase SDR

smcogs
  SMCOG1001: short-chain dehydrogenase/reductase SDR

AKT74273.1: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs
  SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

AKT74275.1: cytochrome P450

smcogs
  SMCOG1007: cytochrome P450

AKT74277.1: pyridine nucleotide-disulfide oxidoreductase

smcogs
  SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

AKT74280.1: cytochrome P450

smcogs
  SMCOG1007: cytochrome P450

AKT74286.1: oxidoreductase

smcogs
  SMCOG1079: oxidoreductase

AKT74289.1: cytochrome P450

smcogs
  SMCOG1007: cytochrome P450

AKT74291.1: acyl-CoA dehydrogenase type 2

smcogs
  SMCOG1191: acyl-CoA dehydrogenase type 2

AKT74293.1: short-chain dehydrogenase/reductase SDR

smcogs
  SMCOG1001: short-chain dehydrogenase/reductase SDR

AKT74294.1: flavin reductase domain protein FMN-binding

smcogs
  SMCOG1160: flavin reductase domain protein FMN-binding

AKT74300.1: short-chain dehydrogenase/reductase SDR

smcogs
  SMCOG1001: short-chain dehydrogenase/reductase SDR

AKT74306.1: cytochrome P450

smcogs
  SMCOG1007: cytochrome P450

Transferases: acylase acyltransferase glycosyltransferase methyltransferase transferase
AKT74256.1: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs
  SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

AKT74259.1: malonyl CoA-acyl carrier protein transacylase

smcogs
  SMCOG1021: malonyl CoA-acyl carrier protein transacylase

AKT74296.1: glycosyltransferase, MGT family

smcogs
  SMCOG1062: glycosyltransferase, MGT family

AKT74299.1: glycosyltransferase, MGT family

smcogs
  SMCOG1062: glycosyltransferase, MGT family

AKT74301.1: O-methyltransferase

smcogs
  SMCOG1042: O-methyltransferase

AKT74303.1: acyltransferase 3

smcogs
  SMCOG1088: acyltransferase 3

AKT74304.1: glycosyltransferase, MGT family

smcogs
  SMCOG1062: glycosyltransferase, MGT family

AKT74305.1: O-methyltransferase

smcogs
  SMCOG1042: O-methyltransferase

AKT74307.1: O-methyltransferase

smcogs
  SMCOG1042: O-methyltransferase

Lyases: cyclase dehydratase
AKT74255.1: NAD-dependent epimerase/dehydratase

smcogs
  SMCOG1010: NAD-dependent epimerase/dehydratase

AKT74267.1: cyclase/dehydrase

smcogs
  SMCOG1128: cyclase/dehydrase

AKT74270.1: L-carnitine dehydratase/bile acid-inducible

smcogs
  SMCOG1189: L-carnitine dehydratase/bile acid-inducible

AKT74282.1: NAD-dependent epimerase/dehydratase

smcogs
  SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase
AKT74258.1: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs
  SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

AKT74261.1: Beta-ketoacyl synthase

smcogs
  SMCOG1093: Beta-ketoacyl synthase

AKT74262.1: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

AKT74266.1: AMP-dependent synthetase and ligase

smcogs
  SMCOG1002: AMP-dependent synthetase and ligase

AKT74268.1: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs
  SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

AKT74269.1: 2-isopropylmalate synthase

smcogs
  SMCOG1271: 2-isopropylmalate synthase

Similar gene clusters
Shows areas that are similar to the current region to a reference database.
Mouseover a score cell in the table to get a breakdown of how the score was calculated.The MIBiG database.
Click on an accession to open that entry in the MIBiG database.
Analysis type:

Reference | T2PKS | PKS-like | betalactone | oligosaccharide | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | --- | --- | --- | ---
BGC0002141.3 |  |  |  |  | 2.23 | PKS | trioxacarcin A | Streptomyces bottropensis DO-45
BGC0000231.5 |  |  |  |  | 1.28 | PKS | griseusin A, griseusin B | Streptomyces griseus
BGC0000234.5 |  |  |  |  | 1.05 | PKS | jadomycin | Streptomyces venezuelae ATCC 10712
BGC0001723.5 |  |  |  |  | 1.03 | PKS | oviedomycin | Streptomyces antibioticus
BGC0001160.5 |  |  |  |  | 0.98 | PKS | 1-nonadecene, (14Z)-1,14-nonadecadiene | Synechococcus sp. PCC 7002
BGC0001164.3 |  |  |  |  | 0.97 | PKS | 1-heptadecene | Cyanothece sp. PCC 7822
BGC0001979.4 |  |  |  |  | 0.88 | PKS | LL-D49194α1 (LLD), LL-D49194β2 | Streptomyces vinaceusdrappus
BGC0001257.3 |  |  |  |  | 0.87 | PKS | 1,3,6,8-tetrahydroxynaphthalene | Nodulisporium sp. ATCC74245
BGC0000272.3 |  |  |  |  | 0.84 | PKS | spore pigment | Streptomyces collinus
BGC0002631.2 |  |  |  |  | 0.83 | ribosomal | Myxarylin | Pyxidicoccus fallax

Reference | Aggregated | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0002141.3 |  | 1.00 | PKS | trioxacarcin A | Streptomyces bottropensis DO-45
BGC0002276.2 |  | 0.67 | NRPS | choline | Aspergillus nidulans FGSC A4
BGC0001160.5 |  | 0.67 | PKS | 1-nonadecene, (14Z)-1,14-nonadecadiene | Synechococcus sp. PCC 7002
BGC0001164.3 |  | 0.66 | PKS | 1-heptadecene | Cyanothece sp. PCC 7822
BGC0000231.5 |  | 0.65 | PKS | griseusin A, griseusin B | Streptomyces griseus
BGC0001257.3 |  | 0.62 | PKS | 1,3,6,8-tetrahydroxynaphthalene | Nodulisporium sp. ATCC74245
BGC0000272.3 |  | 0.62 | PKS | spore pigment | Streptomyces collinus
BGC0000234.5 |  | 0.59 | PKS | jadomycin | Streptomyces venezuelae ATCC 10712
BGC0001197.5 |  | 0.59 | PKS | frankiamicin | Frankia sp. EAN1pec
BGC0000275.4 |  | 0.58 | PKS | tetracenomycin C | Streptomyces glaucescens

Similar known clusters from MIBiG 4.0
Shows clusters from the MIBiG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Detailed help and explanations are available here.
Click on reference genes to show details of similarities to genes within the current region.
Double click on a reference drawing to reverse the display of the genes.
Click on an accession to open that entry in the MIBiG database.
Location:
Identifier
Identity
Coverage
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A domain glossary is available here, and an explanation of the visualisation is available here.
Selected features only
Show module domains
NRPS/PKS substrates
Type II PKS
NRPS/PKS substrate predictions
Shows the predicted substrates for each adenylation domain and acyltransferase within genes. Each gene prediction can be expanded to view detailed predictions of each domain. Each prediction can be expanded to view the predictions by tool (and, for some tools, further expanded for extra details).
AKT74259.1: mal
PKS_AT (215..503): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 49.0
  Methoxymalonyl-CoA: 35.7
  Methylmalonyl-CoA: 27.5
  Propionyl-CoA: 20.7
  inactive: 19.4
  fatty_acid: 12.3
  trans-1,2-CPDA: 0.0
  Isobutyryl-CoA: 0.0
  Ethylmalonyl-CoA: 0.0
  CHC-CoA: 0.0
  Benzoyl-CoA: 0.0
  Acetyl-CoA: 0.0
  3-Methylbutyryl-CoA: 0.0
  2-Methylbutyryl-CoA: 0.0

AKT74266.1: AHBA
CAL_domain (39..472): AHBA
minowa: AHBA

Prediction, score:
  AHBA: 22.5
  shikimic_acid: 17.0
  NH2: 14.4
  Acetyl-CoA: 0.7
  fatty_acid: 0.0

Link to PARAS substrate predictor
Type II PKS gene cluster prediction details
Predictions of starter units, elongations, product classes, and potential molecular weights for type II PKS clusters.
More detailed information is available here.

Cluster section:
  0 to 57534
Starter unit(s):
  2-methylbutyryl-CoA (Score: 992.0; E-value: 0)
Malonyl elongations:
  8|9 (Score: 684.9; E-value: 7.1e-209)
Product class(es):
  angucycline
  anthracycline
  aureolic acid
  tetracycline
Product molecular weight(s):
  2-methylbutyryl-CoA_8: 407 Da
  2-methylbutyryl-CoA_9: 449 Da

AKT74259.1
  AT (Score: 408.3; E-value: 5.7e-125)
  AT ADB23379.1_TLN-05220/TLN-05223_2-methylbutyryl-CoA (Score: 301.0; E-value: 1.1e-97)
AKT74260.1
  ACP (Score: 117.3; E-value: 5.3e-37)
AKT74261.1
  CLF 8|9 (Score: 684.9; E-value: 7.1e-209)
AKT74262.1
  KS (Score: 759.0; E-value: 2.7e-231)
AKT74267.1
  CYC C7-C12 (Score: 427.4; E-value: 5.7e-131)
AKT74268.1
  KSIII (Score: 515.9; E-value: 1.1e-157)
  KSIII AKT74268.1_Trioxacarcin_2-methylbutyryl-CoA (Score: 691.0; E-value: 0.0)
AKT74271.1
  KR (Score: 194.4; E-value: 6.6e-60)